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Learning Outcomes
Evaluate the strengths and weaknesses of the various methods used to construct
physical maps of genomes
Describe how restriction mapping is carried out
Describe how fluorescent in situ hybridization (FISH) is used to construct a
physical map, including the modifications used to increase the sensitivity of this
technique
Explain the basis of sequence tagged site (STS) mapping, and list the various DNA
sequences that can be used as STSs
Describe how radiation hybrids and clone libraries are used in STS mapping
Study Questions
11. Explain how a restriction map is obtained. What special procedures can be used to
increase the size of DNA molecule for which a restriction map can be obtained?
12. What is FISH and how is it used to construct a physical map?
13. Describe the various types of DNA sequence that can be used in STS mapping.
14. Draw a diagram showing how a double-stranded cDNA is synthesized.
15. Define the term 'mapping reagent' and explain how a panel of radiation hybrids is used
as a mapping reagent.
16. Explain how a clone library is used as a mapping reagent.
17. Draw a diagram to show how a sample of a single human chromosome can be obtained by
flow cytometry.