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Universidad de Alcal
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Curso Acadmico 20162017
Apellidos:
Nombre:
Curso / Facultad:
1. Starting an Experiment
To start a new experiment:
a) Select the type of tissue to be placed within the organ bath from the Tissue Type list.
b) Click the New Experiment button.
c) Click the Record button to start the chart recorder running.
d) Nerve stimulation / Select and apply drugs from the list of available Agonists and
Antagonists.
2. Adding Agonists
To add an agonist drug to the organ bath:
a) Select the type of agonist to be applied from the list of available agonists.
b) Select the concentration of the drug solution to be applied from the Stock Soln. list.
c) Enter the volume (between 0 and 1 mL) of the stock solution to be applied into the
Volume box.
d) Click the Add to Organ Bath button to inject the selected volume of the select stock
solution agonist into the organ.
e) When the tissue response on the chart recording reaches a steady state (or after 30
seconds if no response has occurred) click the Flush Reservoir to Bath button to wash
out the agonist from the organ bath.
Note. Agonists are drugs which, when applied to tissue, cause a response (contraction in
the case of the tissues typically studied in organ baths) by binding to specific receptors
on the surface of the cells within the tissue.
3. Adding Antagonists
To add an antagonist drug to the organ bath or Krebs solution reservoir:
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a) Select the type of antagonist to be applied from the list of available antagonists.
b) Select the concentration of the drug solution to be applied from the Stock Soln. list.
c) Enter the volume (between 0 and 1 mL) of the stock solution to be applied into the
Volume box.
d) Select Organ Bath from the Add To list if the antagonist is to be applied directly to
the organ bath. Select Reservoir if the drug is to be added to the Krebs solution
reservoir.
e) Click the Add to button to inject the volume of the selected stock solution agonist
into the organ bath or reservoir.
Note. Antagonists are drugs which block the actions of agonists on tissue, reducing or
preventing the tissue response to the agonist. Application of an antagonist will thus have
no apparent effect on the tissue in the organ bath unless an agonist is present (or the
tissue is being stimulated).
Antagonists are typically studied by observing the effects an antagonist has on different
concentrations of a chosen agonist. To avoid the necessity of repeatedly applying the
antagonist to the organ bath before each agonist, it is usually added to the Krebs
solution reservoir of Krebs solution used to flush the organ bath between agonist
applications.
FBC
The organ volume is 10 mL. Thus, adding 0.1 mL of a 10-4 M stock solution gives a
final bath concentration of 10-6 M.
The bath dilution formula can be rearranged to work out the volume you need to add to
achieve a required FBC:
Volume added
You should aim to add volumes between of 0.1 0.5 mL to the tissue bath (less than
0.1 mL is difficult to measure accurately and more than 0.5 mL starts to increase the
solution volume in the bath significantly).
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RC
The reservoir volume is 1000 mL. Thus, adding 0.1 mL of a 10-3 M stock solution gives
a final bath concentration of 10-7 M.
The dilution formula can be rearranged to work out the volume you need to add to
achieve a required reservoir concentration:
Volume added
You should aim to add volumes greater than 0.1 mL to the reservoir (less than 0.1 mL
is difficult to measure accurately).
7. Menu Options
Printing a chart recording
To print a copy of the displayed chart recording on a printer, select Print from the File
menu.
Copying the chart recording to other programs
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To copy the data points of the displayed chart recording to the Windows clipboard for
pasting into a spreadsheet or graph plotting program, select Copy Data from the Edit
menu.
To copy the a picture of the displayed chart recording to the Windows clipboard for
pasting into a Word document or a PowerPoint presentation, select Copy Image from
the Edit menu.
Saving a chart recording to file
To save the current chart recording for the current experiment to a data file, select Save
Experiment from the File menu and enter the name of a new data file.
Loading an existing chart recording from file
To load a previously saved chart recording from a data file, select Load Experiment
from the File menu and enter the name of the data file.
PASOS
1. Vaya al men de inicio y abra el programa OBSim > OBSim.
2. Una vez abierto el programa de simulacin, examine los siguientes
apartados:
a. Tipo de tejido (Tissue type). Presione ON > New experiment.
b. Ver que en los mens que aparecen dos mens de agonistas y
antagonistas, en los cuales aparecen diferentes frmacos a
distintas concentraciones (nota: no puede aadir ms de 1 mL de
disolucin de frmaco, el volumen para el bao de rganos viene
determinado en 10 mL y el volumen de reserva son 1000 mL).
Despus
presione
Experimental
Setup
para
ver
la
8. Rellene la siguiente tabla y construya finalmente la curva dosisrespuesta representando la fuerza de la contraccin, expresada como
% del efecto mximo, frente al log[histamina] en el bao.
concentracin
(M)
1 10-9
5 10-9
1 10-8
2 10-8
5 10-8
1 10-7
2 10-7
5 10-7
1 10-6
Respuesta
(g)
% Emx
(H sola)
10
Respuesta
(g)
% Emx
(H + antag.)
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Apellidos / Nombre:
Grado:
Curso:
Grupo Prcticas:
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Papel semilogartmico
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