Disclosure to Promote the Right To Information

Whereas the Parliament of India has set out to provide a practical regime of right to
information for citizens to secure access to information under the control of public authorities,
in order to promote transparency and accountability in the working of every public authority,
and whereas the attached publication of the Bureau of Indian Standards is of particular interest
to the public, particularly disadvantaged communities and those engaged in the pursuit of
education and knowledge, the attached public safety standard is made available to promote the
timely dissemination of this information in an accurate manner to the public.
1 +, 1 +

01 ' 5

The Right to Information, The Right to Live

Step Out From the Old to the New

Mazdoor Kisan Shakti Sangathan

Jawaharlal Nehru

IS 5279 (1969): Dicofol Emulsifiable Concentrates [FAD 1:

Pesticides and Pesticides Residue Analysis]

! $ ' +-
Satyanarayan Gangaram Pitroda

Invent a New India Using Knowledge

! > 0 B

BharthariNtiatakam

Knowledge is such a treasure which cannot be stolen

IS : 5279., 1969
(Aeaffirmed 2002)

Indian Standard
SPECIFICATION FOR
DICOFOL EMULSIFIABLE CONCENTRATES
Second Reprint OCTOBER 2007
( Includrng Amendment No.; J 2 &

UDC 632 951 [DICOFOLJ

C Copyright 1969
BUREAU OF INDIAN STANDARDS
MANAK BH.,AVAN, 9 BAHADUR SHAH ZAFAR MARG
,

Gr4

NEW DELHI 110002

December 1969

18.5279.1_

Indian Standard
SPECIFICATION FOR
DICOFOL EMULSIFIABLE CONCENTRATES
Pest Control Sectional Committee, AFCDC 6

RtJwI3""i,.,

Clulimum

Dil"f'Ctorate of Plant Protection, Quarantine & Stor.

(Ministry
Food, Agriculture, Community
Development It Co-operatioa )

DR SARUAR SINOR

or

M-'n,
L. Auv AWl
DR K.. K"LYAH (AI,."",)
Da K. BAGOS!

Tata Fiaan Jndu.tries Limited, Bombay

SHIU ){.

DaH. L. BAlli

;
Ii
Ie

DR B. B. BHATIA

Da N. K. BAIV ( .4lItruu )
MA.J-GBM

M. S. BorAKAI

a. Urban Development

Central Forensic Science Laboratory, Central 8111'

or InveatiKalionl, New Delhi
I. C. I. (Jndia) PrIvate Ltd. Calcuua
Dinetorate General of

.
SaRI A. W.

CoCKY
SRR) H. C. SOMA aHAR (
DlR.croa or AOlllCVL'l't1RJC

Maniltry or Health, Family Plaoninlt Woru. HoUliIll

Armed 'orca Medical

Services, Ministry of ner..nee

Shaw Wallace Ie Company Limited, Calcutta

Allnnal, )

Department of Alriculture. C,ovemment 01 tltar

Pradeah
i
DR P. L CHATVBVZDf ( AllmttJl, )
I
DIRECTOR or STORAGE at 11(8.0- DirHtorate
StOFale cl Inspection (Minilt~ of
TION
.'ood, ......icultW'e. Community Developme
Co-operation )

or

DDun D.aBcroa ( SToRAO.

TxcUKICAL) (

naua. CONTBOu..1IR

AI,.,.." )

Phannaco~i. Committee

(MiDiittY 01
Health, Family PJanninlt Works. HOUlint
Urban Developmeat)
SRR. G. D. GO_HAL.
Bombay Chemicals Private Ltd, Bombay
SHRI V. V. K"'''A a ( AI,.".." )
DR R. GOlCCALntI
National Organic Ch.mical Industries Limited.
Bombay
DR S. B. D. AOA.WALA (AI"""')
DR K. C. GULA'rJ
Indian Al"icultura1 Research Institute, New Dellai
SURI T. GaoRG. Jo..
Hjndustan lnaecticidee Llnlited, New Deihl
D. S. N. NAG ( AI",." )
D. !.Au",. RAJ
Cyanamid ladia Limited, Bombay
SIlRI M. K. ZVTlHl ( AI"".." )
Indian

(c.IiMtM .. ,." 2)
It1REAU

or

INDIAN STANDARDS

MANAK IlHAVAN. 'BAHADUa SHAH ZAFAR MARO

NEW DEUtI J 101m

IS ,5279 ..1969
( Conti"",d from POI' I )

Mmcb"s
SURI

S. K.

/bJlrultJIUaI
Central

M4-JUIIDAR

SKRI M. MITTllt1 ( A11mt4U )

Stun L. P. MA'l'HVn
Saar K. P. StNOJl (Allman/I)

Food Technolosical

( CSIR ), Myeore

Releareh

I natitute

Ministry of Defe-nee ( DGI )

PMtiddtt. As~ociarion of rndia. New Delhi

M1(llTA
8laarat Pulverising ~{ills Pvt Ltd, Bombay
Sli RI S. (~JI t\TT~ltJ I (Alter"ak)
811ftr D. N. NAkliATE
Bayer ( India) Ltd, Bombay
DIt D. V. KA8UKI.IEAR ( Iilt,rIlDII)
SUBI R. NARASIMHAN
Directorate General of Technical Development

On P. R.
SHIU

MEHTA

T. S.

( Miniatry of Industrial Development, at Company

SHRI

S. L.

Affairs)
Anlerican Spring & Pressing "'orks Private Ltd,

PATEL

Bombay
SJlHl 1... l\1. PATEL ( tfltnnat#)
PLANT PnOTEC'1'JON OFFICER
DB S, PaADHAN
DR RATTAN tAL ( All"",," )
SHIll P. ItAKDAR
DIl A. SAlttJP
SllRI A. K. SJ~NOtJPTA

Department

of Agrkulture,

Government of MYlOre

Indian Agricultural Research Institute, New Delhi

(A,ntral Wart'houaing Corporation, New Delhi
Peat Control Corporation, Calcutta
National Malaria Eradication Programme, Delhi
Pest Control ( India) Pvt Ltd, Bombay

Dn VINon R. SUA1.
IJIl H. D, SIU\,ARTAVA
Indian Veterinary Research Institule, Izatnalar
lJn R. P. (;HAvnHURl (AlurnQu)
Dn I... R. Suo
l\finistry of Defence (R " D)
SHJU S. K. AfJ'r.IA (AI/maalt)
Dn B. L. W A'l'TAI.
National Institute of Conununicable Diseases. Delhi
Oil Hvm IlHAG\VAN,
Director General, lSI ( Ex-oJlitio MmaHr)
1)eputy ) lir('(.lor ( Agri & Food)

Sr"ary
E. N. SlH(f).,n
{)eputy Director (Aari &. Food), lSI
S.tRI

Pesticides Subcommittee, AFCDC 6: I

c.....
Oft P. R.

MKHTA

PHticidei Auociation of India, New Delhi

M. . .,
SaRI M. L. AnVANI
DR ~. KALW A N ( .~II'''Ult' )

Tata Fiaon Indultries Limited, Bombay

Sanr V. J. RAK..

Central Revenues Control LaboratorYt New Delhi

AGRICULTURAL CHatl"" A NO
ASSOCIATE PRO. . .oa OW SOIL
S~I1bfCB

Alricultural College &: Research Institute, Coimbatore

SH&JN.X.C.OWDRVBt(A~)

AMENDMENT NO. 1

AUGUST 1972

TO

IS : 5279-1969 SPECIFICATION FOR

DICOFOL EMULSIFIABLE CONCENTRATES
Alteratioll

( PtJ" 4, ,Inrls, 2.3.1 ) - Substitute the follow ing

tor

U,c existing

clause:
, 2.3.1 D"n/ol Content - When determined by the method prosenbed III
vppendrx r:,lhrol~rrvcddicorol content, perl tint h\ weight, of anv ot the
samples shall not drllet Ii om the nouuual "due by more than thE' tolerance
hmus mdu ated below:

SIND.

Nominat V4111c Pr runt

10

i)

up

It)

Above 10 clnd up to SO

iii)

(0

Abov 50

2.3.1.1 1 he

J oleranc: J l1111t
f-

lO

5
3

Peru 171

') of the nominal value

It

JJ
t

tual V,l(Ut' of the t('( hnu al material In the tnrmtldtton

~h(tll 1)(' l all ulated to the c,Plond dPl tnldl place dnd then rounded otlt to thv
Iust decimal pl'll (.I betore etppl) Ing the tolt' f cl n l e "t 1\ en 'lbo\ tJ"
di

;
t

(.uux 0 r

AMENDMENT NO.2

JULY. 1976

TO
SPECIFICATION FOR

IS: 5279-1969
DICOFOL EMULSIFIABLE CONCENTRATES
Alteration
( Pagt 4, clause 2.3.1 (a.. also Amendment No. J)] -Substitute the
followina for the existing clause:
'2.3.1 DieD/til Content-- When determined by the method prescribed in
Appendix C, the observed dicofol content, percent mass/mass of any of the
samples shall not differ from the declared nominal value by more than the
tolerance limits indicated below:
Tolerance Lmut
Percent of the nommal value

Nominal Valu$

Percent

-+

Upto9
t

10 and below

sO:

SO and above

'0
5

::I: 5
+-

i~

2.3.1.1 The actual value of dicofol content shall be caJcuJat~ to the

second decimal place and then rounded ofT to the first decimal p'af before
applying the tolerances as stipulated In 2.3.1.
.)
t

(AFCDC 6)
Printed at S,mco Prant.ng Press. Deihl

AMENDMENT NO. 3 MAY 1994

TO
IS 5279: 1969 SPECIFICATION FOR DICOFOIJ
EMULSIFIABLE CONCENTR~TES

t Pag 5, clau ..e

4.1 ) - Substitute the following for the existing:

'When treshly manufactured matenal in bulk quantity i~ otlcred lor inspection,

representative samples of the matcnal shall he draw It and tested as prescribed in
IS 10627 : 19R3 within 90 days of it", manufacture, When the material i~ offered
for invpcction ;tft("r 90 days of 11\ manufacture, 4\alnphn~ vhall hl' done a,
prrvcribed in IS 10617 : 19S3. HCl\Vl'VCr, the criteria for conformity of the
materiaI \\ hen tested. ~ball he tbe Illuit~ of tolerances, as apphcable over the
declared nominal value and given under clause 2.3.1 of the standard:

(FAD 1)
Printed at Simco Prlnteng Press, Deihl

11,5279.1_

Indian Standard
SPECIFICATION FOR
DICOFOL EMULSIFIABLE CONCENTRATES

o.

FOR E W 0 R D

0.1 This Indian Standard was adopted by the Indian Standards Institution
on 26 September 1969, after the draft finalized by the Pelt Control
Sectional Committee had been approved by the Agricultural and Food
Products Division Council and the Chemical Division Council.
'\

0.2 The emulsifiable ~oni('ntrates formulations of dicofol are used in the

control of acarine pests ofptgricultural importance.
"~

0.3 Taking into consideration the views of producers, consumers, te,sting

authorities and technologists, the Sectional Committee responsible fot the
preparation of this stancl8.rd felt that it should be related to the raanufacturing and trade practices followed in the country in this field.

O.t This standard is one of a series of Indian Standards on

their formulations.

pestirid~ and
,

0.5 This standard contains clauses -3.3 and E-4.4 which call for an Itgreement between the purchaser and the vendor.

0.6 For the purpose of deciding whether a particular requirement of this

standard is complied with, the final value, observed or calculated, expressing the result of a test or analysis, shall be rounded off in ac-cordance with
IS : 21960.. The number of significant places retained in the rounded off
value should be the same as that of the specified value in this standard.

1. SCOPE
1.1 Thil standard prescribes the requirements and the methods of test for
dicofo! emulsifiable concentrates used in the control of acarine pests of
agricultural Importance.
*Ruleslnr roundiftl off numerical valun ( ".11 ).

lI.sm.l.
2. ItEQ,VIRIMENTS
2.1 The material shall consist of dicofol. tecJmical. cliuolvecl iD IUitable
IOlveDt. together with emullifyiDI asent( a) and, when necessary,
atabilizer(. ).

2.1.1 Dicorol, technical, employed in the manufacture of the material,

ahall conform to IS: 5278.1969
2.2 1'IIyUcaI- The material shall comply with the physical requirements
al described in 2.2.1 to 2.2.~

2.2.1 DlstriJllitnt- The material abaIl be in the form of clear able

liquid free from extraneoUI matter.

2.2.2 CoI4 T,,, - No turbidity or separation or aoIicI or both or oily

matter sha)J occur when the material i...ijectecl to the coW test at
AI

lere.

prescribed in Appendix A.
Non-lithe cold lilt . . . . . . . to l- carriecI out at lower ....

........ by .... parcJIMer.

Ire. It .......

u.s FItuIt p,;", (AM) - WheD determiDed by the method P: 20 el

18:1448 (Part 1)-J96Ot. the fIuh pOint 01 the matwial ahaIl be .bove
24~oC.

2.2.4 E.Lri... S,.IIiI. -. The creaminr or the lICIimeDtatioD, if a"~

shall DOt in either c:&Ie exceed 2-0 mI at tbe ~ or bottom 01 100 mI
cylinder. _heR tested by ODe or the methods prelCnbecl in Appeadix IS.
U m..-a The material aWl comply with the chemical requirementa liven in 2.&1 aDd 2.1.2.

2.1.1 J?ilol

c.,.".,- When

d....ined by the IDeIhocI

or

in Appendix C, the oa..wd dicolol coatent. percent by weiaM, 01.,

the aampJea aIIII IIGt difter &om tbe noaaiaaI value by more t1uUa .5 , .
ceat 01 the Iatt.-.

I.U Ariiifl- .4/""- WIaeD ~ by the 1D~ ~esa;w

ill ~ D, alae ~~. if~. calculated ~aulpbwic acid (~,)
or
if . ,. . . . . . . IOdiuIR hydroxicfe (NaOH), .-u De
Bel .ore daD 8-1 perc:.t by weiPL

.au.uaky.

Ie ~4GKING AND 1MB...

.....

.,.y

COD""

1.1 PM!,! , -The IIIaCtriaI IhaII he packed ia Mild ateel

lined with 1MW pIleaolic .IUM
pheaoJic CIt aDOCIiIIcI . . . .
~

et

,.
tcIaaIcaI (..,,,,.).
tor ""
itt~PutI

11.5271.1_
3.1.1 The packing sball allO conform to Tariff Rule1 in force from
time to time for packing of good. falling in the category of materials of

high flash point.

3.2 MarJdaa- The containen lball be securely closed and .hall bear
legibly and indelibly the following information:
a) Name of the material;
b) Name of the manufacturer;
c) Date of manufacture;
d) Batch number;
e) Net volume of contents; and
f) Nominal technical dicofol content, percent by weight, and minimum
cautionary noti~ worded as under:

KEEP AWAY fROM FOODSTUFFS AND ANIMAL FEEDS.

DESTROY EMfTI' CONTAINERS.

3.2.1 The product rna} plliiO be marked with Standard Mark.

1

3.2.2 The use of the Standard Mark is governed by the provisions of the ~ureau
of I "dian Standard~ Act, '986 and the Rules and Regulations made ther~nder
The details of conditions under which the licence for the use of Standar Mark
Ina) be granted to manufactures or producers may be obtained from the ureau
of Indian Standards,
~

4. SAMPLING

4.1 Representative samples of the material shall be drawn

al

prescribed in

Appendix E.

5. TESTS
5.1 Testl shall be carried out by the appropriate methods referred to
in 2.2.2, 2.2.3, 2.2.t, 2.3.1 and 2.3.2.
5.2 (laaIlty .1 Rea,eat. - Unless specified otherwise, pure chemicals
and distilled water (see IS: 1070.1960) shan be employed in tests.
NOD - 'Pure chemicals' shall man chemicals that do Dot contain impurities which
affect the Multi of analysil.
Specification for water, distilled quality (,IfIiI,d).

IS I 5279-1969

APPENDIX A
( Clause 2.2.2 )
COLD TEST
A-I. PROCEDURE
A-I.I Take 50 ml of the material in a l00-ml transparent glass container
and close it with a cork or stopper fitted with the thermometer. <Jool the
material to 100 (se, Note) by placing the container in the water mainrained at go to lOne. When the temperature of the material in the
con taincr reaches I O~C, add to it a small seeding crystal of dicofol with
minimum opening of the stopper within the shortest possible duration of
time. Gently stir the material in the container at intervals for one hour
maintaining the temperature of. the material at 10C. At the end of one
hour J examine the material for any turbidity or separated solid or oily
matter or both.

NO'l'E - In casE' the purchaser Ipecified tbat the telt il required to be carried out at
00, the material in a c.ontainer ahall be cooled to OC by placiD, the container in a
bath of crushed ice and the telt carried out at oec iDitead of 100.

APPENDIX B
(Clause 2.2.4 )
DETERMINATION OF EMULSION STABD..ITY

B-1. METHODS
8-1.1 Two methods are prescribed for the determination of emulsion
stability of the material. The method to be employed shall depend upon
the manufacturer'. instructions for the preparation of the diluted emulsion
from the material. Method 1 shall be employed in the case of a material
which is required to be added to standard hard water Cor the preparation
of the diluted emulsion, while Method 2 shall be employed in the case of
a material to which standard hard water should be added to prepare the
diluted emulsion.

B-2. METHOD 1
11-2.1 Appua..e
11-2.1.1 Btdtr- 250 ml rapaC'ityJ with an internal diam.tft' of 6-0 to
6'5 em and marked at 100 ml,
6

15.5279.1969

8-2.1.2 Moh, TyfJI Pi/JIII,

B-2.1.3 GldSs Rod -4 to 6 mm in diameter and of a convenient length.
8-2.1.4 G,aduaua Cylindn- 100 ml capacity and graduated at each
millilitre from 2 to 100 ml.

B-2.2 Realeat
8-2.2.1 Standard Hard Ii'ater - Dissolve 2-740 g of calcium carbonate
and 0-276 g of magnesium oxide in the minimum of dilute hydrochloric
acid (approximately 2N). Expel the excess acid by evaporation to dryness on a water-bath and dissolve the residue in a small quantity of water.
Tran sfer this solu tion quan titatively to a lOO-ml graduated flask, make up
the volume with water and mix. Pipette 10 ml of this solution into a onelitre graduated flask, make up the volume with water and mix.
NOTE- Standard hard Wat'f is defined al 'water' which provides a hardnEls or 342
parts per million, calculated ~a calcium carbonate and bas the following compositiom

Calcium chloride, anttdroul

0304 I

Magnesium chloride, 'exahydrate

0-139g

Distilled water

To make 1 000 ml

ts

8-2.3 Procedure - Pour

to 80 rnl of standard hard water at a temperature of 30:J: 1C into th~ beaker. By means of the Mohr type pipptte,
add 5 ml of the concentrate to the standard hard water contained iff the
beaker. The material should be added to the standard hard water a the
rate of 25 to 30 rnl per minute, with the pointed end of the pipette 2 em

inside the beaker and aDowing the material to pour directly int the
standard hard water and not along the sides of the beaker. The con nts
of the beaker should be stirred vigorously with a glass rod durin the
addition. Add more water to bring the volume of the diluted ernulsidn to
the lOQ-ml mark with continued stirring and transfer immediately to the
clean and dry graduated cylinder. Keep the cylinder with its contents at a
temperature of 30t> :i-: 1C in a thermostatically controlled bath for half an
hour. After the half hour, note the volume of the creamed matter at the
top and the sediment at the bottom, if any.

B-2.4 Report-- Report the total volume in millilitres of the creamed

matter at the top and the sediment at the bottorn in the cylinder,

8-,. METHOD 2
N.l Proeedan-Place 5 ml of the concentrate in the beaker. Add
sufficient standard hard water at a temperature of 30:: tOe into
dropping funnel and add it to the material contained in the beaker at
rate of 15 to 20 ml per minute. During the addition, continuously stir
contents of the beaker with the glass rod. Stop the addition of

the
the
the

the
standard hard water just when the volume of diluted emulsion in the

IS 1527'.196'
beaker reaches 190 mi. Transfer the diluted emulsion immediately to the
clean and dry graduated cylinder. Keep the cylinder with it. contents at
a temperature of 300 d: 10 in a thermostatically controlled bath for half
an hour. After the half hour J note the volume of the creamed matter at the
top and the sediment at the bottom, if any.
8-3.2 Report - Report the total volume in millllitres of the creamed
matter at the top and the sediment at the bottom in the cylinder.

APPENDIX C
(Clawe 2.3.1 )
DETERMINATION OF DICOFOL CONTENT
0.1. REAGENTS
0-1.1 Eth,1 Alcohol - 95 percent.

0.1.2 EthaaoUe Pota.ll. . Hy. .xlcle-O5 N.

<:-1.3 Coae_taW Nitric Acld ....analytical reagent grade conforming
to 18:2641968.
e-l.f Ferric AI1IID IacUcator- Dissolve 350 g of ferric ammonium
sulphate crystals in 700 ml distilled water, heating gentlyJ if necessary,
Add 50 ml of eoncentrated nitric acid, dilute to 1 litre with distilled

water and mix thoroughly.

c.J.5 Nltrobe..eae
e.I.6 Sta.eI.rd 811y. Nitrate SoladOD - 0'1 N.
0-1.7 Staaclarcl Ammoal TIalocy...te Sol.doa-O'I N.
C.IJI PIa..olpJatlaaleia I_tea- IoI.tlOII-Diaolve 0-5 g of phenolphthalein powder in 100ml of95 percent ethyl alcohol.
C.2.PROCED1JIlB
e-2.1 Weigh accurately sufficient quantity of the material containing 051
of the technical material in an erlenmeyer flask. Add 50 ml of the standard
ethaoolie potassium hydroxide solution to the sample. Place the flask on a
hot.plate and connect the neck of the flask to a water-cooled condenser..
using S to 4 drops of 95 percent ethyl alcohol as a seal. Boil the solution

'Speciflcation tor nitric acid (,m-J).

IS : 52'79 -1969
under gentle reflux for 90 minutes. At the end of this time, stop heating
and allow the flask to cool slightly, rinse the condenser with 25 ml of
95 percent ethyl alcohol. Disconnect the flask and rinse the tip of the
condenser with 95 percent ethyl alcohol, adding the rinsings to the flask.
C-2.2 Drain the liquid contents of the flask into a 4OO-ml beaker, add
50 ml of 95 percent ethyl alcohol to the flask. Swirl the flask, and again
drain the liquid contents into the 4OO-ml beaker. Add approximately
100 ml of distilled water to the flask, swirl, and add the contents of the
flask to tllP 400 ml beaker by stirring. Rinse the flask with small portions
of distilled water and add the rinsings to the 40Q-ml beaker by stirring.
Continue rinsing the fta\k until there is a total volume of approximately
300 ml in the beaker,
0.2.3 Place the beaker on a steam-bath until all of the alcohol has been
evaporated. Cool the btaker in a water-bath and add 2 to 3 drops of
standard phenolphthalein indicator solution and sufficient 1 : 1 nitric acid
to turn the indicator coleurless, Add an additional ) 0 ml of 1:) nitric
acid and 50 rnl of the sta'dard silver nitrate solution. Thoroughly mix the
contents of the beaker. ~dd 5 ml elf ferric alum indicator solution, 5 mlof
nitrobenzene to the bealfr, stir vigorously, and titrate the eXCflSS silver
nitrate with the standar' ammonium thiocyanate solution with vigorous
stirring, until the app~ararc~ is of a faint hut permanent pink end-poipt.

C-3. CALCULATION

e-3.1 Active ingredient, percent content =

(111 AgNoa for sample -nll AgNo3 for blank)
------~- ~ --

weight of sample (grams )

NOTB -

f
X

12-g-1 t

,') 1t;

Run a blank determination through all .t~p. uC the procedure Ul$ng all
'

reagent. except the material to be analyzed.

APPENDIX D
(Clause 2.3.2)
DETERMINATION OF ACIDITY OR ALKALINITY

0.1. Q,UALITATIVE TEST

1).1.1 Proeeclve- Take about 0'5 ml of the material in a test-tube and

mix with about one millilitre of water. Test the mixture for acidity or
alkalinity with a litmus paper. Determine, as the case may bet the acidity
(s" 0.2) or the alkalinity (SIt D.').

527'.1_
1).2. DETERMINATION OP ACIDITY

1).2.1

a..pat.

1).2.1.1 M,'h.11 R,d Indicator Solution-prepared by dissolving 0 15 g of

the indicator in 500 ml of water.
0.2.1.2 Standard Sodium HJt/roxitll 80Iutio,,-002 N.

D-2.1.3 Standard Hydrochloric Acid- 0'02 N.

1).2.2 Proeedare- Weigh accurately about 10 g of the material into a
dry conical flask and dilute with 100 ml of water. Titrate the contents of
the flask immediately with the standard sodium hydroxide solution using
methyl red as the indicator. Alternatively, the end-point may be determined pontentiometricaUy.

D-2.2.1 Carry out a blank determination on 100 mlofwater.

0.2.3 CalealatioD
Acidity (as HISO,), 4-9 (V - v) N
percent by weight ==
W
where
V == volume in ml of the standard sodium hydroxide solution
required for the test,
v == volume in ml of the standard sodium hydroxide solution
required for the blank determination,
N == normality of the standard sodium hydroxide solution, and
H' = weight in g of material taken for the test.
0.2.3.1 In case the blank determination shows alkaline reaction, neutralize with the standard hydrochloric acid and calculate the acidity as
follows:

Acidity (as H.s~,), 4'9 (V.N + vX )

percent by weight ==
~'
whete

V == volume in m1 of the standard sodium hydroxide solution

required for the test,

N, .. normality of the standard sodium hydroxide solution,

v _ volume in ml of the .tandard hydrochloric acid required for

the blank determination,

N. :II normality the standard hydrochloric acid, and
W == weight in g of the material taken for tbe test.

or

10

IS I 5279-1969
D-3. DETERMINATION OF ALKALINITY
1).3.1 R.,eats
0-3.1.1 M,thyl R,d Indicator Solution -prepared by dissolving 0-15 g of
the indicator in 500 ml of water.

0.3.1.2 Standa,d Hydrochloric Acid- 0'02 N.

0.3.1.3 Standard Sodium Hydroxide Solution-O02 N.

0-3.2 Pro~eclure-Wf'igh accurately about 10 g of the material into a
dry conical flask and dilute with 100 ml of water. Titrate the contents of
the flask immediately with the standard hydrochloric acid, using methyl
red as the indicator. Alternatively, the end-point may be determined
potentiometrically.

D:J.2.1 Carry out a blank determinatlon with 100 ml of water.

D-3.3 Calcula\ioll
Alkalinity~as (~aOH), 4'0 (V -LJ) N
percentlby
weight
== ------~.-~ .
~

where

V = volume in 011 of the standard hydrochloric acid required

for the resr,
v = volume in ml of the standard hydrochloric acid req
I'd
for the blank determination,
f
}I =- normality of the standard hydrochloric acid, and
Ji' == weight in g of the material taken for the test.
1

u}r
t

0.3.3.1 In case the blank determination shows an acid reaction,

neutralize with the standard sodium hydroxide solution and calculate the

alkalinity a8 follows:
Alkalinity (as N~OH), 4-0 (V}{t + vN: )
percent by weight
=
IV

where
V c= volume in ml of the standard hydrochloric acid for the test.
Xl - normality of the standard hydrochloric acid,
u

= volume

in m) of the standard sodium hydroxide solution

required for the blank determination,

N. = normality of the standard sodium hydroxide solution. and
f1" == weight in g of the material taken for the test.
11

APPENDIX E
( Clawe 4.1 )
SAMPLING

or DlcorOL

EMULSIFIABLE CONCENTBATU

"1. GENERAL PRECAU110NS

.1.0 In drawing, preparing, storing and handlinl test _mples, the
foflowing precautions and directions shall be observed.
E-l.1 Samples .hall not be taken in an exposed place.

8-1.2 The sampling insteumen; shall be of glass or of a metal on which

the material has no action and shall be clean and dry when used"
-1.3 Proper precautions shall be taken while drawing samples since the
material is highly poisonous.
8-1.. Precautions shall be taken to protect the samples, the material being
sampled, the sampling instruments and the containers for samples from
adventitious contamination.

-1.5 The samples shall be placed in leak-proof lOO-ml aluminium

containers of a minimum purity of 99 'percent.
.1.6 The sample containers shall be of such a size that they are almost,
but not completely-filled by the sample.
B-l.1 Each sample container shall be fitted with an inner stopper, which
shall be sealed in the neck of the container by means of a suitable sealing
composition. The container shall then be securely closed by screw cap
lined with tin foil. The containers shall then be marked with full details
of sampling, the date of manufacture, name of the manufacturer and other

particulars

or the con.ignment.

.1.8 Samples shall be stored in such a manner that the temperature of the

material does not vary unduly from the normal temperature.

.1.9 Samples shall be protected from Jight.

.2. SAMPLING INSTRUMENT

B-2.1 s.mpHaa T.IIe- It shall be made of thick glass or of a metal on
which the material baa no action, and shall be of 20 to 40 mm diameter
and 400 to 800 mm in length (," Fig. 1). Tbe upper and lower ends
are conical and of 5 to 10 mm diameter at the narrow ends. Handling it
facilitated by two rings at the upper end. For taking a sample, the tube is
first closed at the top with. the thumb or a .topper and lowered until the
desired depth il reached. It is then opened for a ahort time to admit the
material and finally closed and withdrawn.

12

18:52191_
....., ,..... TO10 D"

..........- ..

20 TO .0 D'A

e
0->

I
-H-.

TO 10 DIA

All dilMlllica ill .Wimetta

'IG.

1 SAllPLIKO Tun
N.I.I For small containers. a .maller IUDpIins tube
..... may be used.

or suitable dimen-

a-s. 1CAL! or MMPUNG

&&1 !At - All the containen in a aiDlle ~ipment of the material
drawn &om the same batch or manufacture ahall CODllitute a lot. If.
eoDsipment ia declared or knowD to CODsist

or different betches or manu-

factaie, the containers beJouliftl to the _ e batch shall be sroupecl

topth. and each such group shall constitute. teparate lot.
B-S.I.I Samples .hall be tested foreacla lot forucertain.illl the conformity
of the material to the requirement. or thi. specification.
a.&2 Tbe number (JI) of containers to be cholen from the lot thaD d.nd
OR the size of the lot (.~) and shall be in accordance with col I and 2
ofT.hle I.
13

IS .5271969
-3.3 These containers shall be chosen at random from the lot and in order
to ensure the randomness of selection, some random number table as
agreed to between the purchaser and the vendor, shall be used. In case
such a table is not available, the following procedure shall be adopted:
Starting from any container in the lot, count them as 1, 2, 3.... ,
up to " in a systematic manner, where' is equal to the integral part of
the value of Nln, N being the total number of containers in the lot and
n the number of containers to be chosen (st' Table 1). Every rth
container thus counted shall be separated until the requisite number of
containers is obtained from the lot to give the samples for test.
TABLE 1 NUMBER. 01' CONTAINERS TO BE CHOSEN POR SAMPLING
( Claus,s E..3.2 and E-3.3)

No. OJ'

LOT SIZE

CONTAINRas
'1'0 BE
u8EN

AI'

en

n
(2)
3
4-

(I)

3 to 15
16 ., 40

5
1

41 u 65
66 " 110
Over 110

10

E4. TEST SAMPLES AND REFEREE SAMPLES

E-f.l Before drawing the test samples, mix thoroughly the contents of each
container selected by shaking or stirrin~ by suitable means, or by rolling.
so as to bring all portions into uniform distribution. Draw by inserting the
sampling instrument through the bung-hole or any other convenient
opening, small portions of the material from different parts of each selected
container. The total quantity of the material drawn from each container

shall be not less than 350 ml,

1...2 Mix thoroughly all the material drawn from the same container. A
small but equal quantity shall be taken from the each selected contai.nerand
shall be well mixed topther 80 al to form a sample of not Jess than 450 ml.
Thi. sample shall be divided into three equal composite test samples. one
for the purchaser, another For the vendor and thethird for the referee. .

w.s The remaining material drawn from a

container (after the quantity

needed for formation of the composite teat sample bas been taken out) shall
be divided into three equal partl. These parts shall be immediately tranlferred separately to thoroughly dried bottles which are then sealed air-tisht
with 8to~rl and labelled with all the particulars of sampli", given
under ~1.7. The material in each such sealed bottle .hall constitute an
Il\dividual teat sample. These individual test samples ahaD be separated
14

IS: 5279.1969
into three identical sets of individual test samples in such a way that each
set has a sample representing each selected container. One of these three
sets shall be marked for the purchaser, another for the vendor and the
third for the referee.
&-4 Releree Sample. - Referee samples shall consist of the composite
test sample (see E-4.2) and a set of individual tE'st samples (Sft E-4.3)
marked for this purpose and shall bear the seals of the purchaser and the
vendor. These shall be kept at a place agreed to between the two.

E5. TESTING OF SAMPLES

-5.0 The number of tests mentioned in E-5.1 and E-S.2 shall he first
conducted on the test samples mar ked for the purchaser. In the rom ingencies, 8\1<..h as IOSSt spoilage and spilling, of the purchaser's test samples
or if the vendor so desires, the test sample's marked for the vendor may be
tested. In case of a dispute, the referee test samples shall be tested and
the test results obtained On the referee test samples shall be considered
as final.
E-5.1 Tests for the determination of dicofol content and emulsion stability
shall be conducted on eac' of the individual samples i see E-f.3 ).
.5.2 Tests for the deter~inatjon of the remaining characteristics, namely
cold test, flash point, and .acidity or alkalinity shall be conducted 01) the
composite test samples (sef EA.2).
;

J"

&.6. CRITERIA FOR CONFORMITY

E-6.1 A lot shall be declared as conforming to this specification wher
a) each of the test results for emulsion stability and dicofol co~tent
satisfies the corresponding requirements specified in 2.2.4 and t-3.1.
b) the test results on the composite test sample for cold test, ~ash
point, and acidity or alkalinity, satisfy the corresponding requirements given in 2.
.6.1.1 In case one or more of thE' test results do not satisfy the requirements for dieofol content and emulsion stability, the following procedure
shall be adopted for determining the conformity of the material in respect
of these characteristics, The mean and range of the corresponding test
results shall be calculated as:
Sum of the test r~sults
Mean (X) == Number of test results

Range (R)

= Difference

between the highest and the lowest value

obtained for the test results.

The appropriate expression as shown in col 6 of Table 2 shall be

calculated. If the values of these expressions satisfy the relevant condition
as given in col 6 of Table 2 the lot shall be declared to have satisfied the
requirements for dicofol content and emulsion stability.

15

II.S27t1961
T..... 2 CIUTDIA POR CONIOUIITY
(a.. 1-6.11)

SL

CHABAOTDlmca

(I)

(2)

No.

"'TRavvre

MIld

!lA.G.

CBlft&I01f "_
eo.'OBlllft

(3)

(4)

(5)

(6)

R,

I, 2, .....

1) Dicofol content

~ :I: 0-6 R1 aball

lie within ttie nomi.
naI value :1:5 % or
the nominal value

X,

Ii) Emulaion ItabUit,

R. .f; + &6 a.

<2-0

(~/"/MI'2)

.......,

Da H. L.

BAlli

.",...,
CeDtral ForeIWe Science Labontary. OeDlra1 BuNMI
01I nVeltiptionl. New Delhi
I. C. I. ( lpdia ) Private Ltd. CaIcu...

D. B.B. B."'rIA
Da N. K. 8u ( ....._)
DR S. L. CHOPRA
Punjab Aarieultural UllivenJty.L~~~
SM81 M. S. DIiAn
National Malaria Endicalioa ~ . Delhi
Sa.1 G. D. GO.HAUI
Bombay Chemicals Private Ltd. lOmba,
SI V. V. Kas ( .AI"".", )
D. 1t.. GO.CALYM
NatioDal Olpnic Chemical IncNatri. Limhecl.
Ion.ba,
J)a S. I. D. AoWALA (A""')
Da K. C. GUL.~I
Indian Alricultural ReseArCh IMtitute. New Delhi
Da S. DllwA. (.AlIIntatI)
SOl T. O...".~..
HilaclUitu IDleCticWea Limited. New DeIW

_at

DB S. N.
(MIItwII)
K."
1...
a.emiatry DlpartllleDt, Delhi UDiv",. Delhi
Sa.1 T. S. M.ftA
Pulv...... Milia Private Limited, Bo...,
ilia. L 0. .,...,1 (.."".,,)
.... D. P. MrtN
MiIUIcry at OCJ 1l a: D}
NAG

D.

"AbU.

baa It.

NA.AaI....

Da &. D. rAldA
D. It. I. 'kAlA.........
Da v. S"DA8l....
Da PI' k. SARIIA
D" V.lIOD k. aa'8
Ilnll

R. D.

S....

DirectOrate
0( Techftlal ~ t
( Miniltry of JacI.&rW DeveIopmeaI Canapeay
.wain)

DirecIerale 01Plat ProsecdoR, (bIINDClM a. s.....

~at Pood, ~..... e.-ueky

...

a..1 K..It. euAT. (~)

Oa 8. L WA"'r.

Co-otwauoa )

itecI ~
....., M.nicij;l CerP:ntioa, . . . .,
L I. D.. - Parry Limited. ,.....
. . ContlOl { India ) Private Ltd.
JDCbaItnes LiIaiIed,

"ba, 10m.,

Nationallaadtute oleo.tamuaicaWe ~ Dellai

.... V. N. BR..b"'A1' (..." .",)

16

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