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Anatomy & Histology, Flinders University of South Australia, Adelaide, South Australia 5001
Environmental Biology, University of Adelaide, Adelaide, South Australia 5005
3
Department of Physiology, Monash University, Clayton, Victoria, Australia 3800
2
Abstract
Pelicans produce altricial chicks that develop into some of the largest birds capable of sustained flight. We traced
pulmonary morphological development in the Australian pelican, Pelicanus conspicillatus, from third trimester embryos
to adults. We described growth and development with allometric relationships between lung components and
body mass or lung volume, according to the equation y = ax b. Pelican lung volume increased faster than body mass
(b = 1.07). Relative to lung volume, the airways and vascular spaces increased allometrically (b > 1) in embryos, but
isometrically (b 1) after hatching. Parabronchial mantle volume decreased (b < 1) prior to hatching and increased
isometrically thereafter. Surface area of air capillaries, blood capillaries and the bloodgas barrier increased relative
to lung volume (b > 0.67) before and after hatching. Barrier thickness decreased before hatching, remained constant
in juveniles and decreased by adulthood. The anatomical diffusing capacity significantly increased before hatching
(b = 4.44) and after hatching (b = 1.26). Although altricial pelicans developed pulmonary complexity later than precocial
turkeys, the volume-specific characteristics were similar. However, lungs of volant adult pelicans became significantly
larger, with a greater capacity for gas exchange, than lungs of terrestrial turkeys. Exchange characteristics of growing pelican lungs were inferior to those of adult birds of 26 other species, but converged with them at maturity.
Key words altricial; bird; development; morphometry; pulmonary.
Introduction
Pulmonary morphometry has been shown to be a
valuable approach for understanding the links between
form and function in relation to the evolution of animal
design and behaviour (Maina, 2002). In particular,
morphometric techniques have led to the identification of the functional subunits involved in determining
diffusing capacity, which is the primary measurement
of pulmonary function. They have further provided the
basis for pulmonary modelling, which allows analysis of
the design of the gas exchanger in relation to its func-
Correspondence
Dr Sue Runciman, Department of Anatomy & Histology, Flinders
University of South Australia, GPO Box 2100, Adelaide, South Australia
5001. E: sue.runciman@flinders.edu.au
Deceased.
Accepted for publication 29 June 2005
Anatomical Society of Great Britain and Ireland 2005
Morphometry
A four-level cascade sampling design (Cruz-Orive &
Weibel, 1981) was employed as follows. Sampling cascade
levels and phases of interest: level 1, parenchymal and
non-parenchymal (large blood vessels, bronchi, connective tissue) volume at 4 magnification; level 2,
parenchymal composition (secondary and tertiary
parabronchi, atria and interparabronchial mantle) volume
at 20 magnification; level 3, air and blood capillary
volume and surface densities at 8000 magnification;
level 4, the tissue barrier at up to 32 000. Morphometric
measurements as described by Weibel (1979) were
used to estimate volume and surface densities.
Light microscopy
Point counts were carried out at 4 and 20 magnification. Counts at low magnification were carried out on
all lung sections of the smaller lungs. Counts on the
larger lungs and counts at 20 magnification were
carried out on sections and fields sampled using a systematic sampling technique (Weibel & Cruz-Orive, 1991). A
minimum of ten entire sections at 4 magnification and
a minimum of 20 fields at 20 magnification were
counted. Images of the sampled sections and fields were
captured by using a Canon EOS-1 camera mounted on
an Olympus BX50 microscope and imported into Coreldraw 9 (Corel Corp.). Grids were superimposed on the
images. Images at 4 magnification were used to determine the volume densities of the non-parenchyma
(Vvnp) and parenchyma (Vvp). The non-parenchymal
component included bronchi (Vvbr), large blood vessels (Vvbl) with a diameter greater than 25 m and connective tissue (Vvct). The parenchymal component of
the lung included parabronchial lumina (Vvpbl), atria
(Vvatr), interparabronchial tissue (Vvpm), interatrial
septa (Vvias) and small blood vessels (Vvsbv). Volume
densities of the parenchymal components were determined at 20 magnification.
Results
The morphological changes that occur during lung
development are illustrated in Fig. 1. At day 24 of incu-
Fig. 1 All sections were stained with Haematoxylin and Eosin and are at the same magnification. a, atria; bv, blood vessels;
pb, parabronchi; m, mesenchymal tissue; sb, secondary bronchus. Arrowheads indicate smooth muscle cells. Arrows denote
infundibula. (a) The lung in the pelican embryo at 24 days of incubation. Parabronchi with narrow lumina can be seen in the loose
mesenchymal tissue. The cluster of cells indicated by the asterisk is the site of development of a new parabronchus. (b) At internal
pipping, about 2 days before hatching, shallow atria open from the parabronchi. The interatrial septa are thick and have
club-shaped luminal tips. Infundibula indicated by arrows extend into the parabronchial mantle that contains a small amount
of mesenchymal tissue and blood vessels. Small-diameter airspaces in the mantle may be air capillaries or infundibula. The
arrowheads indicate a layer of smooth muscle underlying the parabronchial epithelium in a region where atria have not yet
developed. (c) In the hatchling lung, atria have deepened, infundibula are more common and a few small-diameter airspaces
are seen. The parabronchial tissue is markedly reduced and there is little mesenchymal tissue. (d) At 11 days after hatching the
thickness of the parabronchial mantle (pm) has increased. Atria and infundibula are well developed and lead into air capillaries
that are surrounded by a network of blood capillaries. Smooth muscle cells are seen at the luminal tips of the interatrial septa.
Small areas of undifferentiated tissue are seen in the parabronchial mantle (*). (e) At 21 days after hatching atria are deep and
infundibula extend far into the parabronchial mantle. Blood vessels, indicated by the asterisks, are located at the bases of the
interatrial septa. The mantle contains a network of air and blood capillaries as well as larger air spaces and is still very cellular.
There is no interparabronchial septum of connective tissue. (f) In the adult lung interatrial septa have thinned but have retained
their club shape due to the group of smooth muscle cells at the tip. This smooth muscle forms a ring around the openings of the
atria and provides structural support maintaining the patency of the atria (Duncker, 1972). The parabronchial mantle is thick,
and the cellularity has largely been replaced by a network of air and blood capillaries. Blood vessels are seen at the bases of the
interatrial septa (*). Infundibula extend and branch deep into the parabronchial mantle.
vessels and large airways. There was no interparabronchial septum and the mantles of the adjacent parabronchi merged (Fig. 1cf).
Body mass (Mb), total lung volume (VL), absolute
volumes, surface areas and thicknesses are presented
Anatomical Society of Great Britain and Ireland 2005
R2 = 0.999, Vnp = 0.255VL0.990.04, R2 = 0.99) with no significant difference in slope between P1 and P2 for Vp
(P = 0.13) or for Vnp (P = 0.10).
Within Vnp, the volume of the bronchi (Vbr) increased
significantly more rapidly relative to lung volume in P1
than in P2 (ANCOVA P = 0.003), as did the volume of large
blood vessels (Vlbv) (P = 0.001) (Fig. 4a,b). In P1, the
slopes for Vbr (b = 1.53 0.47) and Vlbv (b = 1.85 0.54)
were significantly greater than 1, indicating that these
variables increased more rapidly than the lung as a
whole. In P2, both Vbr (b = 0.93 0.10) and Vlbv (b =
1.06 0.12) scaled isometrically with lung volume. There
was no difference in slope between the two parameters
in either P1 (P = 0.33) or P2 (P = 0.09). Within Vp, results
for parabronchi and secondary bronchi, which do not
differ structurally, were combined (Maina, 2002). Increase
in the volume of the parabronchial lumen (Vpbl) increased
faster than lung volume in P1 (b = 1.51 0.25) and
slowed in P2 to increase isometrically with lung volume
(b = 0.99 0.07) (Fig. 4c).
The slope for parabronchial mantle volume (Vpm) in
P1 (b = 0.79 0.09) was significantly less than unity,
indicating that it increased more slowly than lung
volume (Fig. 5a). In P2, however, Vpm scaled isometrically
with lung volume (b = 1.02 0.04). There was a significant change in slope at hatching (P < 0.0001). Small
blood vessel volume (Vsbv) increased much more rapidly in relation to lung volume in P1 (b = 2.24 0.41),
but became isometric in P2 (b = 0.95 0.23). The difference in slope for P1 and P2 was significant (P < 0.0001)
Anatomical Society of Great Britain and Ireland 2005
Table 1 Age, body mass (Mb; g), lung volume (VL; cm3) and absolute volumes (V; cm3) of components of the lung in the
pelican determined with light microscopy. Yolk-free mass is given for all embryos up to internal pipping (IP) and for hatchlings.
Vp, parenchyma; Vpbl, parabronchial lumen; Vatr, atria; Vpm, parabronchial mantle; Vsbv, small blood vessels;
Vnp, non-parenchyma; Vbr, large airways; Vlbv, large blood vessels; Vct, connective tissue
Age
Mass
VL
Vp
Vpbl
Vatr
Vpm
Vsbv
Vnp
Vbr
Vlbv
Vct
Embryos
24 days
16.24
19.36
21.73
24.68
63.85
49.9
67.11
97.26
88.03
110.19
122.24
123.19
553.1
905.8
1870
1994
3724
4181
5541
6821
8834
7190
0.28
0.44
0.52
0.76
1.62
1.4
1.28
1.72
2.4
2.54
3.36
2.78
20.38
23.46
49.88
49.36
93.92
153.04
210.32
228.063
267.7
316.35
0.211
0.307
0.380
0.574
1.343
0.935
0.873
1.191
1.882
2.014
2.696
2.112
15.704
16.902
38.124
39.393
67.386
103.204
170.929
161.948
209.453
247.393
0.020
0.026
0.093
0.120
0.339
0.175
0.246
0.458
0.572
0.651
0.819
0.587
3.772
2.984
8.964
13.323
26.022
29.339
43.017
46.304
65.629
58.962
0
0
0
0
0.115
0.080
0.045
0.065
0.217
0.171
0.176
0.312
1.417
1.954
3.201
5.463
7.841
6.731
8.262
12.209
8.029
7.422
0.191
0.275
0.280
0.446
0.860
0.665
0.561
0.612
0.976
1.086
1.500
1.106
9.372
10.722
24.679
19.361
31.250
61.439
107.970
86.387
123.577
173.175
0
0.003
0.008
0.007
0.028
0.015
0.022
0.056
0.117
0.106
0.201
0.107
1.143
1.241
1.281
1.246
2.273
5.695
11.680
17.047
12.218
7.834
0.069
0.133
0.140
0.186
0.277
0.465
0.407
0.529
0.518
0.526
0.664
0.668
4.676
6.558
11.756
9.967
26.534
49.836
39.391
66.115
58.247
68.957
0.005
0.013
0.067
0.054
0.138
0.265
0.142
0.351
0.224
0.329
0.277
0.241
2.033
1.909
3.637
4.983
11.549
8.241
11.255
29.717
14.120
23.460
0.004
0.004
0.010
0.021
0.022
0.085
0.091
0.033
0.251
0.105
0.373
0.318
2.440
3.972
7.794
4.351
12.599
32.178
22.509
23.049
25.299
29.147
0.059
0.116
0.063
0.111
0.117
0.115
0.174
0.145
0.043
0.092
0.014
0.109
0.203
0.677
0.325
0.633
2.386
9.418
5.627
13.344
18.827
16.351
Embryos
30 days
Embryos
IP
Hatchlings
11-day
chicks
21-day
chicks
Juveniles
Adult
Table 2 Age, mass (g), volumes (V; cm3) and surface areas (S; cm2) of components of the lung and harmonic mean thickness of
the bloodgas tissue barrier (ht; m) in the pelican determined with transmission electron microscopy. Va, air capillary; Vt, tissue;
Vc, blood capillary; Sa, air capillary; St, bloodgas tissue barrier; Sctot; total capillary
Age
Mass
VL
Va
Vt
Vc
Sa
St
Sctot
ht
Embryos
30 days
Embryos
IP
Hatchlings
63.85
49.9
67.11
97.26
88.03
110.19
123.19
553.1
905.8
1870
1994
3724
4181
5541
6821
8834
7190
1.62
1.4
1.28
1.72
2.4
2.54
2.78
20.38
23.46
49.88
49.36
93.92
153.04
210.32
228.06
267.7
316.35
0.175
0.212
0.195
0.217
0.482
0.342
0.624
4.923
5.076
13.720
10.480
16.147
35.245
60.289
57.319
70.576
113.239
0.638
0.417
0.325
0.359
0.309
0.556
0.403
2.387
2.891
7.441
6.453
9.409
11.784
21.749
21.738
24.909
17.421
0.047
0.036
0.040
0.036
0.184
0.188
0.113
2.062
2.756
3.519
2.426
5.712
14.410
25.932
23.854
28.092
42.532
482.26
348.13
534.80
593.44
1086.32
1050.67
1296.36
11667.07
11672.58
35421.34
29780.82
46180.10
98586.52
181932.28
167762.44
168925.84
401625.65
88.21
52.26
159.76
132.58
571.18
451.82
475.33
5430.25
6293.63
10535.43
13517.90
26514.89
61765.95
114603.51
113445.66
116472.99
271133.12
293.48
75.05
310.45
277.78
835.10
1027.36
1086.26
9568.25
16278.61
26382.59
19126.42
37878.41
75246.91
146664.83
151822.30
155321.56
309441.15
2.88
1.28
1.14
1.244
0.452
0.349
0.553
0.352
0.445
0.439
0.683
0.581
0.466
0.421
0.415
0.450
0.175
11-day
chicks
21-day
chicks
Juveniles
Adult
Discussion
This study quantifies the morphological changes occurring during development of the lung in the Australian
pelican from the 24th day of incubation through to
maturity. Comparison of the data from this altricial
species and a precocial species with a different pattern
of locomotion, behaviour and metabolic demands
allows identification of maturational differences and similarities in development and growth of lung components
involved in gas exchange. Comparison of the regression analyses of the developing lung in the pelican
with those for mature birds provides insight into
the developmental requirements of the pulmonary
system for strong flight in one of the largest flying
birds.
Aspects of our work on altricial pelicans are comparable with only one other published investigation of a
developing bird, fortuitously on the similarly sized, but
highly precocial turkey Meleagris gallopavo (Timmwood
et al. 1987). Three phases of pulmonary growth have
been identified in the turkey. During the pre-hatch
phase of tissue proliferation, lung volume grows more
rapidly than body mass, and air and blood components
grow more rapidly than lung volume. In the early
post-hatch period of equilibrated growth, lung volume
increases slowly in comparison with body mass, and
Anatomical Society of Great Britain and Ireland 2005
mass and rapid development of air capillaries in relation to lung volume. Compared with the large transition at hatching, however, the transition from the
second to the third phase is minor, gradual and difficult
volume and the large airways (Fig. 4a), large and small
blood vessels (Figs 4b and 5b) and parabronchi (Fig. 4c)
indicate that the number, orientation and arrangement
of these components are established prior to hatching
in the pelican. After hatching the slope for these
lung components is isometric, indicating proportional
increases.
In the turkey, the large airways appear to be established by the 22nd day of a 28-day incubation period,
and further development is less than isometric (b =
0.79 0.04) in relation to lung volume (Fig. 4a). From
30 days of incubation to maturity in the pelican, large
airways occupy relatively more of the lung than in
the turkey (P = 0.05). In P1, with a lung volume less
than 50 cm3, large airway volume is significantly
larger in relation to lung volume in comparison with
large airway volume in mature birds (P = 0.05). The
points for juvenile and mature pelicans would lie
on the extrapolated regression line for mature birds
(Fig. 4a).
Large blood vessel development is similar to the
development of the airways, with the pattern being
established prior to hatching and increasing proportionately with lung volume after hatching. The slope
for large blood vessels in the pre-hatch turkeys is similar to that in the pelicans, suggesting that the number
and orientation of large blood vessels in the turkey
may occur later in the incubation period than large airway development (Fig. 4b). Large blood vessel volume
is significantly smaller in relation to lung volume in the
Anatomical Society of Great Britain and Ireland 2005
pre-hatch pelicans in comparison with large blood vessel volume in mature birds (P = 0.05). In the post-hatch
period, the proportion of the lung occupied by large
blood vessels is similar in pelicans and turkeys and
matched that of the adult bird group.
New parabronchi continue to appear during the
10 days before hatching in the pelican, but the data
from the turkey suggest that the number of parabronchi is established by 6 days prior to hatching (Fig. 4c).
Parabronchial volume in relation to lung volume is
significantly lower in pelicans at all ages than in adult
birds and in turkeys (P = 0.05).
In pelicans, establishment of the parabronchi within
the parenchyma in the pre-hatch period occurs at the
expense of the parabronchial mantle tissue, its volume
increasing relatively slowly in relation to lung volume
in the pre-hatch period and isometrically with lung
volume in the post-hatch period (Fig. 5a). Parabronchial
mantle volume makes up a significantly greater proportion of the lung in pelicans than in the turkeys at
all ages (P = 0.05). In pelicans older than 11 days, with
lung volumes larger than 24 cm3, the proportion of
parabronchial mantle volume to lung volume is larger
than in the adult bird group.
Small blood vessel volume increases more rapidly in
the pre-hatch pelicans than in the pre-hatch turkeys,
but from the time of hatching onwards, small blood
vessel volume in relation to lung volume is similar in
the two species (P = 0.05). Comparison with adult birds
is not possible as data are not available (Fig. 5b).
Conclusions
This study supports previous observations on the accelerated development of lung components in the period
prior to hatching (Duncker, 1972, 1977; Timmwood
et al. 1987). This rapid development coincides with the
transition between chorioallantoic and pulmonary gas
exchange. After oxygen uptake via the chorioallantois
plateaus in both precocial species and altricial pelicans
(Vleck & Bucher, 1998; Pearson et al. 2002), the embryo
internally pips into the air cell and begins to ventilate
the lungs. Chorioallantoic gas exchange diminishes and
pulmonary gas exchange increases during the period
between internal pipping and hatching (Visschedijk,
1968). In precocial turkeys, there is an initial rapid
development of parameters required for respiration
followed by slower development after hatching. In
altricial pelicans, the pattern of development of lung
components is similar to turkeys until hatching, but they
continue to develop rapidly after hatching, many reaching
values that exceed those of turkeys and match or exceed
those in adult birds in general. These developmental
differences are consistent with expected differences
between altricial and precocial species and reflect differences in metabolic demands for locomotion in adults.
Anatomical Society of Great Britain and Ireland 2005
Acknowledgements
This work was supported by the Australian Research
Council. The experiments were performed with the
approval of the Animal Ethics Committee of the
University of Adelaide (S-49-1999A).
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