Abstracts Book Part II

National Conference on Trends in Nanobiotechnology
SP-001
ROLE OF NANOTECHNOLOGY IN FOOD

PATHOGEN DETECTION
Aanchal Johari and Asha Kawatra
Department of Foods and Nutrition, CCS HAU, Hisar
Email: johariaanchal@gmail.com
Detection and analysis of pathogen in foods is critical for food safety, public health,
agriculture, etc. Pathogens are capable of inducing infectious diseases with symptoms
which may manifest within minutes, after a couple of hours to days or even years after
initial infection. Infectious diseases cause significant human pathogenesis and mortality
throughout the world, surpassing cardiovascular diseases and cancer. Though century old
conventional diagnostic methods are reliable but these approaches are slow, not adaptable
to high throughput operations and these methods provide relatively little information about
pathogens. Techniques like spectrophotometer, calorimeter, ELISA, and mass
spectrometry etc., are available but these do not measure up to very minimal levels of
pathogens. Despite substantial progress made in diagnostic field, there is still a need for
faster, cost effective diagnostic tool with high sensitivity and specificity like
nanotechnology. Nanotechnology offers many technological advances for pathogen
detection and it can revolutionize way how food pathogen and infectious diseases have
been diagnosed so far. Nanoscale materials can be customized by changing size, shape,
composition, surface modification and are able to detect presence of pathogenic agent in
food, clinical or environmental samples. A particular nanosystem, like a nanoparticle of
100 nm in size including coating and targeting ligands, can be designed to sense targets
that might be smaller (i.e. lipids), bigger (i.e. bacteria) or of equal size (viruses) to
nanoparticle. There would be different interaction with targets of different sizes, resulting
in unique response patterns which would thereby be helpful in the detection of food
pathogens.
1|Abstract & Souvenirs (Supplementary)
SP-002
SCREENING OF RHIZOBACTERIA FOR PLANT

GROWTH PROMOTION AND THEIR USE IN
NANOAGRICULTURE
Anju Sehrawat and S.S. Sindhu
Department of Microbiology, CCS Haryana Agricultural University, Hisar-125 004
Email: anjusehrawat90@gmail.com
The increasing demand for crop production with reduction in use of synthetic chemical
fertilizers and pesticides is a big challenge now-a-days. Plant growth promoting
rhizobacteria (PGPR) having the ability to inhibit the growth of phytopathogens alongwith
plant growth stimulation characteristics could play an important role in sustainable
agriculture. In the present study, 206 rhizobacterial isolates were obtained from the
rhizosphere of chickpea and screened against Fusarium oxysporum on agar plate assay.
Selected 23 isolates possessing antagonistic property were further characterized for plant
growth promoting attributes such as phosphate solubilization, production of indole acetic
acid, hydrogen cyanide (HCN), siderophore, amino-levulinic acid (ALA) and 1-amino
cyclopropane carboxylic acid (ACC) utilization. All the isolates utilized ACC and
produced ALA. Siderophore production was observed in 48% of the isolates, 39% isolates
produced indole acetic acid and 21% isolates produced HCN. Different isolates showed
variable effects on the germination and seedling growth of chickpea on 0.8% soil extract
agar plates. Recently, nanofertilizers have been found to improve the soil health by
decreasing the toxic effects of chemical fertilizers and nanotechnology inclusion in the
agricultural sector may reduce the damages to the ecosystem alongwith improving crop
productivity. Use of nanotechnology could improve the efficacy of PGPR characterized in
this study for their application as biofertilizers and biopesticides to support agricultural
sustainability.
Abstract & Souvenirs (Supplementary) |2
SP-003
MANAGEMENT OF RICE DISEASES THROUGH

MOLECULAR AND RECENT APPROACHES
Annie Khanna, Kushal Raj and Pooja Sangwan
Department of Plant Pathology, CCS Haryana Agricultural University, Hisar
Email: anniekhanna30@gmail.com
Rice (Oryza sativa L.) is an important cereal crop serving as staple food for more than
half of the world population. About 90 per cent of world rice is grown and consumed in
Asia. India has the largest area under rice and is the second largest producer of rice after
China. Rice is attacked by more than 70 diseases caused by fungi, bacteria, viruses and
phytoplasma. Among these, sheath blight (Rhizoctonia solani), blast (Pyricularia
oryzae), bacterial leaf blight (Xanthomonas oryzae pv oryzae), RTBV, RTSV are most
devastating diseases throughout rice growing regions of the world. For the management
of the rice diseases, it is important to develop accurate and quick method to detect and
diagnose the pathogen in the plant. Based on antibody antigen reaction serological
methods viz., ELISA, LAR are developed and based on sequence information various
methods like PCR, RT-PCR, RT-LAMP, Microarray are developed which are sensitive,
rapid and are able to differentiate between the closely related viruses. Recent techniques
such as real time RT-PCR is being used to quantify the pathogen in the target samples
and monitoring population dynamics. Different strategies like coat protein, replication
enzyme mediated, RNA interference mechanism is used in developing rice transgenic
plants against RTBV and RTSV. Among the recent approaches like nanotechnology has
amplified the effectiveness of silver particles as antimicrobial agents. Silver is known to
attack a broad range of biological processes in microorganisms including cell membrane
structure and functions. Silver also inhibits the expression of proteins associated with
ATP production. Silver nanoparticles can be used effectively in the control of rice blast
disease and the prevention of deleterious infections, even though no phytotoxicity
appeared on rice. Thus, the nanoscale materials have emerged as novel antimicrobial
agents owing to their high surface area to volume ratio and the unique chemical and
physical properties, which increases their contact with microbes and their ability to
permeate cells. In the era to come nanotechnology are expected to play important role for
disease diagnosis and management through nano fungicides in the integrated disease
management.
SP-004
APPLICATIONS OF NANOTECHNOLOGY TO
MITIGATE ABIOTIC AND BIOTIC STRESSES
Gagandeep Singh & Pushpa Kharb
Dept. of MBBB, CCSHAU Hisar
Email: drgagan.10@gmail.com
Abiotic stresses are major constrains which adversely affect the crop productivity and
plant growth. Among all abiotic stresses, drought and salinity are most widespread and
commonly experienced stresses. According to FAO reports, there is a major challenge
among scientific community to increase 70% more food crop production towards world
agriculture for an additional 2.3 billion people by 2050 worldwide. Therefore in such
changing environmental scenario there is a constant need to identify the new area of
research to overcome the technological challenges in addressing the yield barrier, resource
use efficiency and development of environmentally accepted technology. In the recent
years nanobiotechnology is gaining momentum to be occupying the promising position to
mitigate the constraints associated with abiotic and biotic stresses to obtain a sustainable
and secure future of agriculture worldwide. Several metal or metal oxide based
nanoparticles are being studied to assess their potential in plant growth and development,
protection from biotic and abiotic stresses, production and role in modulating the various
processes in plants.
SP-005
MANAGEMENT OF RHIZOCTONIA SOLANI: A

PHYTOPATHOGENIC FUNGI THROUGH
NANOTECHNOLOGY
Gurav N.P., Basavaraj K., Surjeet Singh and Naresh Mehta
Department of Plant Pathology, CCS HAU, Hisar
Email: narayangurav28@gmail.com
Rhizoctonia solani is a widespread, soil- borne fungal pathogen, known to cause various
plant diseases like collar rot, root rot, damping off, fruit and seed decay and different types
of foliar blights. Different types of fungicides are recommended for control of these fungi.
Now a days, the fungicides used for disease control irrationally posed danger to living
system, beside their non-target effects, these are becoming more expensive. To preserve
biodiversity, it is becoming necessary to reassess our strategies and achieve disease
management by alternate approaches such as nanotechnology. The antifungal activity of
several carbon nanomaterials has been known, the single-walled carbon nanotubes
(SWCNTs) has the strongest antifungal activity against different fungal plant pathogens
followed by multiwalled carbon nanotubes (MWCNTs) and graphene oxide (GO). Silver
nano particles disrupt transport systems including ion efflux. The dysfunction of ion efflux
can cause rapid accumulation of silver ions, interrupting cellular processes at their lower
concentrations such as metabolism and respiration by reacting with molecules. Silver ion
are also known to produce reactive oxygen species via their reaction with oxygen which
are detrimental to cells, causing damage to proteins, lipids and nucleic acids. The
antifungal activity of silver is much higher than that of other metals because Ag (I) ions
cause the inactivation of cell wall thiol groups resulting in disruption of transmembrane
energy metabolism. Hexaconazole NPs (100 nm) stabilized by polyethylene glycol (PEG)
are more potential than bulk hexaconazole, exhibited the stability comparable with that of
the conventional formulation of fungicide performed suppression of growth and sclerotial
body initiation. Thus, nanoparticle-based management of Rhizoctonia pathogen is ecofriendly and economical.
SP-006
INFLUENCE OF CHEMICAL PARAMETERS ON

BIOSURFACTANT PRODUCTION BY BACTERIAL
ISOLATES
Harpreet Kaur, Seema Sangwan and Leela Wati
Department of Microbiology, COBS&H, CCS Haryana Agricultural University, Hisar 125004, India
Email: kamraharpreet@gmail.com
Recognizing the importance of developing eco-friendly methods for the synthesis of

biologically active nanoparticles, additional use of biosurfactants as capping agents is
catching up fast. Biosurfactants have emerged as a green alternative for enhancing both
nanoparticles synthesis as well as stabilization. These offer lower toxicity, inherent good
biodegradability and ecological acceptability over chemical surfactants. Their commercial
production, however, is restricted due to increased cost of production which could be
lowered down by optimization of production process using high yielding microbial strains.
Three promising bacterial isolates BK23, BK34 and BK68 were subjected to optimization
of chemical parameters for biosurfactant production using different carbon and nitrogen
sources. Isolate BK68 gave highest 8.0cm oil displacement and 37.50% E24 index and
respective values for isolate BK23 were 7.8cm and 17.00%, by using 2% olive oil as a
carbon source. Further, biosurfactant production increased with increasing concentration of
olive oil from 0.2 to 2.0%. Incorporation of 0.5% urea as nitrogen source in fermentation
medium increased the biosurfactant production as evident from highest oil displacement of
9.3cm and E24 index equivalent to 70.0% in case of isolate BK68 followed by 9.1cm and
77.5% by isolate BK34. Incorporation of 0.5% urea also improved biomass production to
4.02fold in isolate BK23 after 4 days of incubation followed by BK34 (3.18fold) and
BK68 (2.43fold) after 4-5 days of incubation. Among all the nitrogen sources 0.5% (w/v)
urea was found statistically at par with 0.25% and 0.75% (w/v) concentration.
SP-007
STRATEGIES TO COMBINE CISGENIC AND

NANO-TECHNOLOGICAL TOOLS FOR GENETIC
IMPROVEMENT OF CROP PLANTS
Jagdeep Singh, A.K Chhabra
Department of Genetics and Plant Breeding, CCSHAU, Hisar, 125004
Email: jagdeep.gpb2011@gmail.com
Development of improved crop varieties in terms of yield, disease resistance and quality
traits is a continuous process and is the primary responsibility and challenge for a plant
breeder. The traditional plant breeding methods though have been in use since beginning
but have many constraints that make it a slow process. In general, it takes 6 to 8 years to
develop, release and popularize a variety among its grower. The diagnostic methods for
high yield (correlation based studies); good quality (lab studies) and disease resistance
(field screening in sick plots) make the process of variety development slower. The
biotechnological tools like marker assisted breeding (MAS) and development of transgenic
plant have helped a lot to a breeder in speeding up this process but with constraints.
Development of nano diagnostic tools such as nano-fluidics, nanomaterials, bioanalytical
nanosensors etc, has the potential for improving plant breeding programme by overcoming
problems linked to breeding for resistance, production and identification of transgenic
plants. Nano-fluidics such as Open Array or the Fluidigm Dynamic Array technologies
provides PCR mixes for huge molecular breeding assays. Greater precision breeding has
also become possible because of nano genomics-based methods which enable a breeders
to identify useful genes scattered among distant plant this is because of the fact that these
methods enable nanoparticles , nanofibres and nanocapsules to carry the foreign
DNA/desirable genes. Nanotechnology can also specifically target specific plant pathology
problems in agriculture such as plant pathogen interactions and provide new techniques for
crop disease control. If a breeder combines cisgenesis with nanotechnology, the ethical and
moral issues involved with the release of transgenic crops and also be resolved. The
strategies to use nano technological tools in combinations with cisgenic tools will be
discussed in detail in this presentation.
SP-008
SALT TOLERANCE BY SILVER NANOPARTICLES

Kritika Sharma, Rizwana Rehsawla
Department of Molecular Biology, Biotechnology and Bioinformatics
Email: Kritikasharma6894@gmail.com
Salinity is the major abiotic stresses that dramatically threaten the food supply in the
world. Zainab M. Almutairi examined the effects of AgNP dose on the salt tolerance of
tomato (Solanum lycopersicum L.) plants during germination. Silver nanoparticles
(AgNPs) are used to enhance seed germination and plant growth, improve photosynthetic
quantum efficiency and act as antimicrobial agents to manage plant diseases. Tomato seeds
were treated with different AgNP doses and germinated under salinity stress. Five
concentrations of AgNPs (0.05, 0.5, 1.5, 2 and 2.5 mg L-1) and two levels of NaCl (150
and 100 mM) were tested. Seed germination and seedling growth of tomato plants were
markedly inhibited by salt stress, and this effect was alleviated by exposure to AgNPs. The
germination percentage, germination rate, root length and seedling fresh and dry weight of
tomato were improved after exposure to AgNPs under NaCl stress. The expression of salt
stress genes was investigated by semi-quantitative RT-PCR. Four salt stress genes, AREB,
MAPK2, P5CS and CRK1, were up-regulated by AgNPs under salt stress, and three genes,
TAS14, DDF2 and ZFHD1, were down-regulated in response to AgNPs. The expression of
other salt stress genes varied between the two salinity levels NaCl150 and NaCl200.The
gene expression patterns associated with AgNP exposure suggest the potential
involvement of AgNPs in response to stress, indicating that they might be useful for
improving plant tolerance to salinity.
SP-009
IDENTIFICATION OF BIOSURFACTANT
PRODUCING BACTERIA ISOLATED FROM
HYDROCARBON POLLUTED ENVIRONMENT
Kavita Rani, Seema Sangwan, Leela Wati
Department of Microbiology, CCS Haryana Agricultural University, Hisar 125004, India
Email: kavita260892@gmail.com
Development of an efficient experimental protocol for the biosynthesis of nanomaterials is

one of the challenging topics in current nanotechnology. Biosurfactants were recently
emerged as potential candidates for biosynthesis of metallic nanoparticles in an ecofriendly
manner. Six biosurfactant producing bacterial isolates, BK23, BK34, BK48, BK58, BK66
and B68, isolated from hydrocarbon contaminated water and soil samples were assessed
for biosurfactant production abilities in terms of emulsification index using MS broth
supplemented with 2% diesel as a carbon source. Highest production was observed in case
of isolate BK68 (18.0%), followed by isolates BK23 (15.0%), BK34 (15.0%), BK48
(14.0%), BK58 (14.0%) and BK66 (12.5%). All the isolates were subjected to
identification based on biochemical methods. Isolates BK23, BK58 and BK68 were found
to be aerobic, G+ve and endospore forming bacteria, therefore, may be identified as
Bacillus spp. Isolate BK66 being G+ve, coccus (in bunches), catalase +ve, and showing haemolysis may be identified as Staphylococcus spp. Remaining two isolates, BK34 and
BK48 were G-ve, rods, oxidase +ve and glucose fermentation ve, therefore, may be
identified as Pseudomonas spp. Isolate BK68 was recognized as highest biosurfactant
producing bacteria which could be recommended for bioremediation as well as
biosynthesis of nanoparticles following enhancement of biosurfactant production abilities
by optimization of cultural parameters.
SP-010
ROLE OF NANOTECHNOLOGY IN
ENTOMOLOGICAL RESEARCH
Meenu
Department of Entomology, CCSHAU, Hisar
Email: mmeenu17@gmail.com
Nanotechnology refers to controlling, building, and restructuring materials and devices on

the scale of atoms and molecules. A nanometer (nm) is one-billionth of a meter. Several
species of ants recognize magnetic signals with the help of magnetite nanoparticles.
Synthesis of leaf mediated silver nanoparticles especially with Pongamia pinnata is a
potential substitute for the existing organophosphorus insecticides like Tenophos,
malathion and fenthion etc., for mosquito control programme. A nanogel has been
prepared from a pheromone, methyl eugenol (ME) using a low-molecular mass gelator.
Notably the involvement of the nanogelled pheromone brought about an effective
management of oriental fruit fly, Bactrocera dorsalis, a prevalent harmful pest for a
number of fruits including guava. Nanosensors and nano-based smart delivery systems
could help in the efficient use of agricultural natural resources like water, nutrients and
chemicals through precision farming. Through the use of nanomaterials and GPS with
satellite imaging of fields, remotely detect crop pests or evidence of stress such as drought.
Nano-encapsulation includes the use of a different kind of nanoparticles with insecticide.
In this process a chemical such as an insecticide is slowly but efficiently released to a
particular host plant for insect pest control. Nano-encapsulation with nanoparticles in form
of pesticide allows for proper absorption of the chemical into the plants unlike the case of
larger particles.
A b s t r a c t & S o u v e n i r s ( S u p p l e m e n t a r y ) | 10
SP-011
NANOTECHNOLOGY FOR PLANT MITES

CONTROL
Monika Jangra, Rachna Gulati, Vinod Batra1, Sonika
Department of Zoology, 1Department of Vegatable Science
CCS Haryana Agricultural University Hisar 125004
Email: monika.jangra81@gmail.com
Nanotechnology is emerging as a rapidly growing field with its application in science and
technology for the purpose of manufacturing new materials at the nanoscale level. The
potential uses and benefits of nanotechnology are enormous. These include management of
insect pests through the formulations of nanomaterials-based insecticides. The plant mites
are one of the most important arthropod pests of agricultural systems. One of the major
obstacles to control them is their ability to rapidly develop resistance to many important
acaricides, even after only a few applications. Spider mite (Tetranychus urticae) has
evolved resistance to more than 80 acaricides to date. Therefore, nanotechnology has
become one of the most promising new approaches for control these pests in recent years.
Nanosulfur is ten times more effective for control of mites as compared to its water
dispersible powder (WDP) formulations. Silver nano particles are effective against T.
urticae when they are put on the surface rather than penetrating way. Cypermethrin and
alpha Terhienyl mixed with nano-particled zinc oxide and copper oxide was effective on
the pear rust mite. The action of mixture of two nanoparticles with two insecticides against
Epitrimerus pyri (Nalepa) is also effective. Applications of chemical pesticides have
adverse effects on animals and human beings apart from the decline in soil fertility.
Therefore, nanotechnology would provide green and efficient alternatives for the
management of plant mite pests in agriculture without harming the nature.
11 | A b s t r a c t & S o u v e n i r s ( S u p p l e m e n t a r y )
SP-012
NANOTECHNOLOGY: NEW FRONTIERS IN

AGRICULTURE
Narender Kumar
Assistant Professor of Physics, Dayanand College, Hisar
Nanotechnology is science of manipulating materials at nano-scale. It occupies a central

position among other latest technological advancements. Agriculture in 21st century faces
diverse challenges to produce more food and fibre to feed a growing population with a
smaller rural labour force, changing climate and urbanization. The use of nanotechnology
in agriculture is getting importance because its possible advantages vary from enhanced
food values, reduced agricultural inputs, improved nutrient contents and longer shelf life.
There are many applications of nanotechnology in agriculture at all stages of production,
processing, storing, packaging. This technology will reduce use of herbicides, pesticides
and fertilizers with increased efficiency, controlled release and targeted delivery will lead
to precision farming. Dream of automated, centrally controlled agriculture can become
reality now. Modern agriculture is need of the hour because conventional agricultural will
not be able to feed an ever increasing population with depleting resources and shrinking
landscape.
SP-013
ROLE OF NANOTECHNOLOGY IN AGRICULTURE

FOR THE MANAGEMENT OF INSECT PEST
Pooja, Asha Sharma, Annu Devi
Department of Botany, M.D.University, Rohtak (Haryana) 124001
Email: tomarpooja2801@gmail.com
Nanotechnology is mainly used for the materials having the size between 1100nm.Nanotechnology has so many properties and due to these properties these
nanoparticles
have
so
many
applications
in
different
fields
like
electronic,medicine,engineering and agriculture. Current scenario of agriculture is changed
due to nanotechnology. Metal nanoparticles and nanobiosensors and many smart delivery
systems are used to fight against the plant pathogens. Like Porous hollow silica
nanoparticles (PHSNs) loaded with validamycin (pesticide) can be used for efficient
delivery system of water-soluble pesticide for its controlled release. Such controlled
release behaviour of PHSNs makes it a promising carrier in agriculture, especially for
pesticide controlled. Nano-silica is prepared from silica has so many applications in
medicine and drug development as catalyst and most importantly is that it can be used as
nano-pesticide.The development of new insect resistant varieties developed with the help
of Nanoparticle-mediated gene transfer. Antimicrobial activity of metal nanoparticles
like copper and silver nanoparticles has been investigated by some researchers against
the plant pathogens. So, it can also be concluded that nanotechnology can provide green
and eco-friendly alternatives for insect pest management without harming the nature.
SP-014
USE OF NANOMATERIALS IN WHEAT TO

MITIGATE ABIOTIC STRESS
Pooja1, Kirpa Ram1 and Renu Munjal2
Department of Botany and Plant Physiology, 2Department of Genetics and Plant Breeding, CCS Haryana
Agricultural University, Hisar
Email: swamipooja709@gmail.com
Major constrains which adversely affect the plant growth and productivity are abiotic
stresses. Therefore the prime concern towards sustainable agriculture and crop production
is augmentation of stress tolerance in plants. Nano biotechnology is gaining promising
position in recent years to mitigate abiotic stress. Silicon Nanoparticles (NPs) are absorbed
by plants and lead to increased stress resistance. A product by Syngenta under the brand
name Primo MAXX is being used as plant growth regulator to tolerate stresses like
drought, heat and disease.Application TiO2 nanoparticles have positive impact on growth
and yield components in wheat under water deficit condition. Application of appropriate
concentration (1.0 mM) of sodium silicate can partially mollify the deleterious effects of
drought stress in wheat also silicon partially increase the leaf chlorophyll contents,
maintained leaf water potential in stressed plants. It also reduces membrane lipid
peroxidation in wheat. Titanium nanoparticle foliar application on wheat have promising
effects on certain agronomic traits like seed gluten and starch contents of wheat under
drought condition also 0.02% titanium dioxide nano particles exhibited enhancement in
plant height, ear weight, ear number, seed number, 1000-seed weight, yield, biomass,
harvest index including gluten and starch content under drought stress. Silver nanoparticles
(SNPs) can stimulate wheat growth and yield. High Soil applied with 25 ppm SNPs had
highly favorable growth promoting effects on wheat growth and yield. Judicious use of
soil applied SNPs can improve yield of wheat crop. Nanocomposites (clay-polyester,
humus-polyester and plastic starch) are safe for wheat germination, emergence and growth
of seedlings.
SP-015
DECIPHERING THE POTENTIAL OF

NANOTECHNOLOGY IN AGRICULTURE
Pooja1 and Ravi Mehndiratta2
Department of Genetics and Plant Breeding, 2 Department of Molecular Biology, Biotechnology and
Bioinformatics, CCS Haryana Agricultural University, Hisar
Email: deepooja16@gmail.com
Nanotechnology is the Design, Fabrication and Utilization of materials, devices and

systems through control of matter on the nanometeric length scale and exploitation of
novel phenomena and properties at the length scale. By taking advantage of quantum-level
properties nanotechnology allows for unprecedented control of material world, at the
nanosacale, providing the means by which systems and materials can be built with exact
specifications and characteristics, allowing materials to be lighter, stronger, smarter,
cheaper, cleaner and more precise. Nanotechnology has the potential to advance
agricultural productivity through genetic improvement of plants, delivery of genes and
drug molecules to specific sites at cellular levels, and nano-array based gene technologies
for gene expressions in plants and animals under stress conditions. The potential is
increasing with suitable techniques and sensors being identified for precision agriculture,
natural resource management, early detection of pathogens and contaminants in food
products, smart delivery systems for agrochemicals like fertilizers and pesticides, smart
systems integration for food processing, packaging and other areas like monitoring
agricultural and food system security. Further developments in nanotechnology in this
sector can be expected to become the main economic driving forces in the long run and
benefit consumers, producers, farmers, ecosystems and the general society at large. In
India, the importance of research and development in nanotechnology has been recognized
as of paramount importance. It is important that the nanotechnology research is extended
to the agricultural total production-consumption systems, that is, across the entire
agricultural value chain. Nanotechnology will give rise to a host of novel social, ethical,
philosophical and legal issues. It is important to have a regulatory mechanism in place to
predict and work to alleviate anticipated problems.
SP-016
NANOTECHNOLOGY AND SCOPE FOR PLANT

DISEASE MANAGEMENT
Prashant Chauhan1, Ravika2, Yogesh2
Department of Plant Pathology, 2Department of Genetics and Plant Breeding, CCS Haryana Agricultural
University, Hisar-125004
Email: pchauhan.k@gmail.com
Nanotechnology has wide applications in various fields of science and technology. The
nanoparticles reduced to size of 1-100nm. The reduction in size, therefore, also changes
the nature of action of particles according to their application. In the field of plant
pathology disease management has been a challenge since ages. With the progress of
knowledge about the nature and mode of actions of plant pathogens, disease management
strategies have been developed timely. However, chemical or biological control
measurements have their limits of applicability. Application of nanotechnology is novel
and a fancy approach in the area of study of plant disease management. Use of DNA
directed silver nanoparticles can significantly affect the viability of pathogens and reduces
the risk of disease progress. Antimicrobial activity of chemical agents could be increased
by developing nanomaterials. Nanomaterials could be used as better carrier agent for
different pathways that includes use of pheromones, systemic acquired resistance (SAR)
inducing chemicals, polyamine synthesis inhibitors and pesticides for their control release.
Developments in nanomaterials synthesis, such as polymeric, carbon and metallic based
have ample scope to apply in managing plant diseases. However, toxicity considerations
and environmental effects are the major concerns to design nanomaterials by tuning the
size, shape and surface modification.
SP-017
EXTRACTION & CHARACTERIZATION OF

CHLOROPHYLL FROM MICROALGAE CHLORELLA
VULGARIS
Prashant Sahni and Rajiv Aneja
Abca Bio Solutions Private Limted, Mohali, Punjab
Email: ftech.sahni@gmail.com
Chlorella is unicellular green algae found in many aquatic systems and it is considered as
potential source of nutrients. Pigments are considered as high market value products.
Chlorophyll is a prominent algal pigment and finds its application as natural colourant in
food and beverage. In the present study chlorophyll was extracted from microalgae
Chlorella vulgaris using methanol at 65 degree C. The characterization of pigment
revealed that it contained carotenoids & chlorophyll; and chlorophyll a and b among
chlorophyll. The amount of chlorophyll was 7.77 mg/g. Pigment retention at acidic pH
ranged from 100% to 93.92% at pH range 6 to 2 whereas pigment retention ranged from
100% to 97.37% from temperature range 30 degree C to 100 degree C.
SP-018
MOLECULAR AND NANO APPROACHES FOR

IMPROVING DROUGHT TOLERANCE AND WATER
UPTAKE EFFICIENCY IN RICE
Rahul Kumar Meena*, Bhoyar P. I., Rajinder Kumar Jain and B. S. Mehla
Department of Molecular Biology, Biotechnology & Bioinformatics, CCS HAU, Hisar
Email: rahulbt24@gmail.com
Molecular marker and Nanoscience based technologies have great potential for improving
drought tolerance and water uptake efficiency in plants. Rice (Oryza sativa L. 2n=24)
belongs to family Poaceae, which is a profligate user of water. To tackle the problem of
water scarcity, research has been directed towards the development of aerobic rice
varieties that combine the drought resistant characteristics of upland varieties with the high
yielding traits of lowland varieties. In aerobic rice varieties, roots grow deeper and more
profusely in comparison to conventional rice varieties, which leads to better absorption of
water thereby eliminating the problem of water logging and methane emission. In the
present study, crosses were made between Basmati (Improved Basmati 370 and Pusa1121)
and aerobic (MAS25, MASARB25 and MAS26) rice varieties with the objective to select
high yielding aerobic rice lines with intact Basmati rice traits. From these cross over 400
F3 plants were raised and evaluated in the field and net house and promising F3 plants were
selected .Out of 396 plants analysed 98 plants had Basmati specific allele at BAD2 aroma
locus. About 60 promising F3 plants has been selected, which had higher grain yield and
biomass. Besides, molecular approach Nanotechnology implying nanomaterial of silicon
and silver nitrate has been used for increasing the WUE in crops like wheat, maize. These
nanomaterials can be used to study if WUE is the selection aerobic lines can be further
increased.
SP-019
ROLE OF NANOTECHNOLOGY IN DEVELOPMENT

OF TRANSGENIC PLANTS FOR ABIOTIC STRESS
TOLERANCE
Rakshita Singh and Pushpa Kharb
Department of Molecular Biology, Biotechnology& Bioinformatics
College of Basic Sciences and Humanities, CCSHAU, Hisar (125004) Haryana, INDIA
Email: singhrakshita19@gmail.com
Plants are adversely affected by abiotic stresses. Drought and salinity are the most
widespread and damaging of all environmental stresses. At the global level, significant
proportion of cultivable land masses is affected by high salt levels. Heat and cold stresses
profoundly affect agricultural yields of major crops. Also, the level of abiotic stresses is on
the rise due to both natural and man-made interventions. Due to the increased levels of
CO2 and other greenhouse gases the ambient temperature is gradually increasing. The
episodes of drought and flooding stress have become more erratic over the years. The
production of transgenic crops that can withstand increased level of abiotic stresses is a
silver lining to sustain and increase food production in future. Techniques of producing
transgenic crops need to be improvised to achieve high frequency transformation. Current
experiments deploying nanotechnology tools for gene delivery are extremely relevant in
production of new generation of transgenic plants. With tools like nanoparticles, carbon
based nanotubes and lipofectins it would be possible to experimentally produce higher
number of transgenic lines and screen out the transgenic lines showing desired phenotype
with greater efficiency. Moreover nanotechnology based biotransformation is relatively
efficient and its combination with commonly applied approaches in transgenic plant
development can minimize the chances of transgene silencing. An improved understanding
of the interactions between nanoparticles and plant responses, including their uptake,
localization, and activity, can revolutionize crop production and help in future endeavors.
SP-020
ADVANCEMENT IN NANOTECHNOLOGY AS
BIONANOPARTICLES
Reena Antil, Pushpa Dahiya
Department of Botany, M.D.U. Rohtak, Haryana, India
Email: rinantil@gmail.com
Nanoparticles (NP) are defined as particles with a diameter smaller than 100 nm, are
increasingly used in different applications like industrial applications, medical imaging,
disease diagnoses, drug delivery, cancer treatment, gene therapy. Many people tried to
synthesis nanoparticles with a variety of synthesis methods including chemical reduction,
electrochemical techniques, photochemical reactions, and now a day via green chemistry
route. Green chemistry route nanoparticles called Bionanoparticles. Bionanoparticles is
rapidly growing technology in the nanotechnology, which has economic and ecofriendly
benefits over chemical and physical methods of synthesis. It has also scope for health care
products such as burn dressings, scaffolds, water purification systems, antimicrobial
applications and medical devices and also in agriculture field. The use of plants, microbes,
and fungi in the production of bionanoparticles is leading the way to more environmentally
sound production of nanoparticles. Various plant extracts was assessed for the synthesis of
nanoparticles. The characterization of nanoparticls is with help of Scanning Electron
Microscopy (SEM), Transmission Electron microscope, UV-Vis absorption spectroscopy
analysis, Fourier Transform Infrared (FTIR) analysis etc.
SP-021
NANOTECHNOLOGY IN CROP PROTECTION

Reena Rani, Sunayana, Anil kumarand, Sukhdeep Singh Sivia
Department of Genetics & Plant Breeding, CCS Haryana Agricultural University, Hisar
Email: saharanreena23@gmail.com
Nanotechnology is the understanding and control of matter at dimensions between

approximately 1to100 nanometers. Nanoparticles are smaller in size with more charge and
larger surface area with higher stability and solubility. The biological agents such as plants
and microbes have emerged as cost effective and efficient candidates for the synthesis of
nanoparticles by green synthesis approaches. They have advantages over conventional
chemical methods which are associated with ecotoxicity. Pests including insects, mites,
nematodes and pathogens are the major limiting factor in crop production. The use of
chemical fertilizers, pesticides and other production inputs has resulted in development of
pest and disease resistance by accumulating residue in produce and environmental
pollution. So there is a need to overcome these constraints with the help of precision
farming practices and effective application of nanotechnology. Nanotechnology in crop
protection holds a significant promise in management of insects and pathogens by
controlled and targeted delivery of agrochemicals and also by providing diagnostic tools
for early detection. Nanoparticles are highly stable and are biodegradable and they can be
successfully employed in production of nanocapsules for delivery of pesticides, fertilizers
and other agrochemicals. Nanoscale systems like encapsulation and entrapment of
agrochemicals such as fertilizers, pesticides, herbicides, plant growth regulators and other
active substances by using polymers, dendrimers, surface ionic attachments and other
mechanisms may be used in controlled and slow release of agrochemicals, which allow the
slow uptake of active ingredients and in turn reduces the amount of agrochemical
application by minimizing the input and waste.
SP-022
ADVANCEMENT IN NANODIAGNOSTIC FOR

PLANT PATHOGEN DETECTION
Renu Singh
Former faculty at Department of Sciences (Biotechnology), J. C. D. Memorial College, Sirsa, Haryana

Email: renuboora@gmail.com
Nanotechnology refers to a nanoscale technology, which has promising applications in

day-to-day life. The word nanotechnology is generally used when referring to materials
with the size of 0.1 to 100 nanometers. This technology emphasizes the implications of
individual atoms or molecules or submicron dimensions in terms of their applications to
physical, chemical, and biological systems and eventually their integration into larger
complex systems. Existing molecular diagnostics techniques for example PCR, ELISA,
Immunofluorecence etc. cant be used for on-site plant pathogen detection. In addition to
these direct pathogen identification methods, indirect methods based on plant stress
profiling and plant volatile profiling have also been used for the identification of biotic and
abiotic stresses along with pathogenic diseases in crops. Though all these direct and
indirect methods are quite sensitive and show specificity but the use of costly, sensitive
molecular reagents and time consuming processes limits the use of these techniques. Thus
there is an urgent need of developing a cost effective, fast and ultra sensitive technique for
easy identification of plant pathogens. Nanotechnology and nanodiagnostic have emerged
as one of the most fascinating and rapidly advancing sciences that possess the potential to
revolutionize many disciplines of science, technology, medicine and agriculture. In
agriculture new molecular biology tools such as nonopores are presumed to provide better
disease detection, prevention and treatments in plants. Thus in future the use of
nanosensors and nanoparticles may prove more precise and quick in the disease diagnosis
and plant pathogen detections.
SP-023
ROLE OF NANOPARTICLES IN ALLEVIATING

THE TOXIC EFFECTS OF HEAVY METALS
Ritu Saini, Sweety Sihag and Sunita Jain1
Department of Chemistry and Biochemistry, 1Department of Molecular Biology, Biotechnology and

Bioinformatics, CCS Haryana Agricultual University, Hisar-125004
Email: ritusaini214@gmail.com
Nanoparticles have been introduced as materials having good potential to be extensively

used in biological, medical and agricultural applications. Heavy metal contamination is a
major environmental concern that restricts plant growth. Metals like zinc (Zn), iron (Fe),
copper (Cu), Mn (manganese), Ni (nickel) and Co (cobalt) are essential micronutrients for
all organisms, the uptake of which is highly regulated in order to supply amounts sufficient
for optimal growth while preventing deleterious effects. Engineered nanoparticles (NPs)
like silicon (Si) and gold (Au) have been found highly effective in alleviating metalinduced toxicity in plants. Being smaller in size and large surface area, NPs easily
penetrate into contamination zone and possess strong affinity to the metals. Generation of
reactive oxygen species (ROS) is a common response of plants to almost all the stresses.
These ROS act as a stress signal to activate plants defense system as well as aggravate
cellular damage. NPs at one end induce generation of ROS while on the other scavenge
ROS by mimicking the activities of antioxidative enzymes. Recent reports suggest that
effect of heavy metal toxicity can be ameliorated by addition of NPs. The potential of
nanoparticles to control the metal stresses in plants should be investigated in future studies
at sub-cellular and molecular level and is also coming under increasing scrutiny.
SP-024
EVALUATION OF ANTAGONISTIC
RHIZOBACTERIA AGAINST PHYTOPATHOGENIC
FUNGI AND THEIR PLAUSIBLE EFFECTS WITH
NANOPARTICLES
Ruchi Sharma, S.S. Sindhu
Department of Microbiology, CCS Haryana Agricultural University, Hisar-125004
Email: ruchi9022@gmail.com
Plant diseases cause about 13% losses in crop production globally and fungal plant
pathogens like Alternaria brassicae cause severe global economic losses in oilseed crops.
For the control of phytopathogens in agriculture, synthetic pesticides are applied but the
indiscriminate use of pesticides has resulted in ecological, environmental and human
health hazards. The use of plant growth promoting microorganisms as biocontrol agents
over synthetic chemical pesticides is more efficient and eco-friendly approach in the face
of the global agricultural sustainability. In the present study, 383 rhizobacterial isolates
were isolated from the rhizosphere of mustard at flowering stage. The screening was done
on agar plate assay against A. brassicae and a number of antagonistic rhizobacterial
isolates were selected on the basis of halo-zone formation. Further, pot house experiment
studies showed significant growth inhibition of A. brassicae (p = 0.05) by the inoculation
of selected rhizobacterial isolates on Brassica juncea. Results showed significant alternaria
bight disaease control and these selected bacterial isolates can be used as biopesticides. It
has been reported that ZnO nano particles-based formulations along with different bacteria
could complement existing strategies for improving crop health in field settings. The
pruposed study outlooks the application of nanotechnologies in modern formulation
technologies and the possible utilization of encapsulated biofertilizers for the plant disease
control and sustainable organic agriculture.
SP-025
EXPLORING NANOBIOTECHNOLOGY TO
MITIGATEABIOTIC AND BIOTIC STRESS
Rekha Patel, Sumit Jangra, Asha Rani, Geeta & R.C. Yadav
Department of Molecular Biology, Biotechnology and Bioinformatics, CCS HAU, Hisar
Email: rekhapatelhsr@gmail.com
Abiotic stress is a major constrain which adversely affect crop productivity and plant
growth. Drought and salinity are most widespread stress, among all abiotic stress.
According to FAO reports, increasing food production is a major challenge among
scientific community. To fulfill the demands of increasing productivity a constant need to
identify the new area of research is needed. In the recent years nanobiotechnology is
gaining momentum to occupy the promising position to mitigate the constraints associated
with abiotic and biotic stress to obtain a sustainable and secure future of agriculture
worldwide. Nanotechnology explores wide area and opens large scope for diverse
applications in fields of biotechnology and agricultural production. Nanoparticles can be
synthesize from metal or metal oxide through various approaches i.e. physical,chemical
and biological for their potential in plant growth and development, protection from biotic
and abiotic stresses. Nanoparticles i.e., metal NPs, metal oxide NPs and Carbon Nano
Tubes (CNTs) etc. have been used in the form of nanofertilizers and nanopesticide/
herbicides. The role of nanoparticles to enhanced seed germination, increased root and
shoot length, fruit and crop yield, and substantial increase in vegetative biomass of
seedlings and plants in many crops including maize, wheat, soybean, mustard, mung bean,
tomato, potato, etc has been identified. Theuse of Scientists reported that application of
Goldnanoparticles in B. juncea,showed increase in yield under field
conditions.Researchers found that use of Mg(OH)2 NPs primed seeds showed higher
chlorophyll content, carotenoids content and seed germination percentage as compared to
unprimed seeds under Cd toxicity.It is evident that more investigations are urgently
required to know the type of nanoparticle, size, concentration, and mode of application to
enable its application on large scale for crop improvement.
SP-026
NANO SUPER POWER OF SUNFLOWER OIL:

EMERGING OPTION FOR HYDROGEN FUEL
Sachin Kumari, R.K. Sheoran and Bunty Sharma
Oilseeds Section, Department of Genetics and Plant breeding, CCS HAU,
Hisar-125004, Haryana
Email: Sachin.narwal97@gmail.com
Hydrogen fuel is the new world fuel which is gaining interest of researcher thru out the
world due to its unbeatable quality over the traditional fuels. Many crops have been
tested and quantified to make a cost effective hydrogen fuel. Out of these sunflower is
emerging as a super power to generate hydrogen fuel by using nanotechnology. The
sunflower oil is a combination of stearic acid, palmitic acid, oleic acid and linoleic acid
which are rich in hydrogen molecules that can produce cost effective hydrogen with the
help of the nanotechnology by using a Nano heater, Nano refrigerator, Nano tubes, Nano
plates. The processing of sunflower oil includes conversation of carbon and hydrogen
that are connected with the hydrogen sensor, which tests the purity of hydrogen and
permits the liquid hydrogen only if it is pure into automobile engine. The automobiles
can effectively uses the hydrogen fuel to run it and the emulsion of automobiles is just a
crude oil, so it cannot pollute the air. As hydrogen is stored in negative temperature,
which make it leads to easy accessibility to the consumer and the Nano refrigerator does
not use any gas, therefore it wont cause any environmental pollution. Therefore
hydrogen fuel is the safest and the best option available to save the earth from ozone
depletion and acid rain. Being the second largest producer of sunflower India can be a
major contributor in making effective hydrogen fuel.
SP-027
NANOTECHNOLOGY FOR FORESTRY AND FOREST

PRODUCTS- A REVIEW
Sandeep Arya, K. S. Bangarwa and Karan Singh Ahalawat
KVK Fatehabad, CCS HAU Hisar
Email: sandeeparyahau@gmail.com
Nanotechnology has the potential to benefit all aspects of forestry and forest products:
from plants, forest management, harvesting, forest operations, wood-based products and
application of wood-based products to the understanding of consumer behavior.
Nanotechnology-based sensors will be able to measure the biotic and abiotic conditions to
a level of accuracy that enables precision forestry and landscape management.
Nanosensors incorporated into wood based material could provide an early warning of
disease and pest damage in the forest. Nanosensors will also be utilized in the future to
monitor forest health and enable us to better maintain healthy forests, landscapes and
ecosystems
The forest industry has goals to create new bio-based composites and nanomaterials, and
to achieve improvement in the performance-to-weight ratio of paper and packaging
products through nanotechnology and nanotechnology-enabled new paper features such as
optical, electronic, barrier, sensing thermal and surface texture. Due to its ability to reduce
carbon footprints of petroleum based products, renewable forest-based nanocelluloses,
together with other naturally-occurring nanocelluloses, have been the subject of active
research and development internationally. Often requested by user industries,
nanocellulose has found its way in the research and development of plastics, coatings,
sensors, electronics, automobile body and aerospace materials, medical implants and body
armor. In the future, we can claim plastics, cellular telephones, medical implants, body
armors and flexible displays as forest products.
SP-028
NANOTECHNOLOGY- A NOVEL APPROACH TO

ENHANCE POSTHARVEST LIFE OF HORTICULTURAL
PRODUCE
Sanjay Kumar1, Subhash Chander2, P. K. Nimbolkar2 and Shaili Kumari2
Department of Molecular Biology, Biotechnology & Bioinformatics, COBS&H, CCS Haryana Agricultural
University, 2Division of Fruit Crops, ICAR-IIHR, Bengaluru, 560089
Email: sanjaybhatti2210@gmail.com
Horticultural crops are seems to be susceptible to various postharvest losses which are
detrimental to the economy. Since the human population is increasing day by day
subsequently the demands of food is also increasing. Presently, the most prevalent method
of controlling decay of produce is the rapid cooling after harvest and storage at low
temperature. Thus, there is an urgent need to have alternative scientific novel technologies
in order to minimize the undesirable physico-chemical and physiological changes of
horticultural produce during storage. Among various technologies, application of
nanotechnology in this aspect could be a novel approach through which production
efficiency can be increased by decreasing the post-harvest losses of horticultural produce.
Nanotechnology can be applied in postharvest operations such as drying, storage and
preservation of fruits and vegetables. The shelf life of the produce can be enhanced by
controlling microbial activity, use of suitable packaging material having good strength and
control over gases and radiations and using multiple chips (Nano-biosensors) to label
products for detection of quality and spoilage. So by understanding the properties of matter
at the Nano scale and controlling it at this level could be a better step towards the shelf life
enhancement of the horticultural produce. In addition to beneficial effects on
physicochemical and physiological quality of produce, the nano-packing materials have
advantages of simple processing and industrial feasibility. Future research is required to
explore the exact nano-packing mechanism during storage to facilitate the application of
nanotechnology over a wider range.
SP-029
APPLICATION OF NANOBIOSENSORS IN PESTICIDE

DETECTION
Sankara Rao, K., Shalini Pandey, Poonia R
Department of Entomology, College of Agriculture, CCSHAU, Hisar
Email: ksankararao0987@gmail.com
The extensive use of pesticides to protect agricultural crops necessitates reliable tools for
the detection of residues in food and water, thus ensuring environmental protection and
consumer safety. Highly sensitive and specific chromatographic detection methods, such
as liquid chromatography (LC), gas chromatography (GC), mass spectrometry (MS) or
combinations thereof (GCMS or LCMS), are available but these are expensive and
time-consuming and currently not suitable for field use. Keeping this view recent studies
have been focusing on biosensor based detection of pesticides. The biosensor
quantitatively estimates resultant product of biological reaction by converting into a
measurable signal. Great progress has recently been made in applying nanomaterialsto
sensor and biosensor development which enable us to detect pesticides up to the
nanolevels.
Different types of nanomaterials, including metal nanoparticles (e.g., gold nanoparticles
(AuNP's)) and semiconductor nanoparticles (e.g., quantum dots (QDs)), have been
widely used as versatile labels in immunoassays and enable superior signal amplification
compared to traditional labels (e.g., organic dyes).For example Chen et al.(2010)
detected 11 nM/mL of chlorpyriphos using CdTe QDs. Guo et al.,(2009) also detected
0.14 M of carbofuran and 0.012 M of triazophos using AuNP'sin drinking water
samples. Nanomaterial acts as signal transducers, electroactive tags in several enzyme
based detection techniques. Monitoring studies on acetylcholinesterase inhibition
resulted in estimation of commonly using Organophosphorus (OP) and carbamate
insecticides in different samples (Aragay et al., 2012). Hossain et al. (2009) reported the
lower detection limit of 1-10 nM of OP and carbamate insecticides in food and beverage
samples. Zheng et al., (2009) detected 0.005 nM of parathion in spiked vegetable and
fruit samples. In this regard, pesticide-monitoring systems based on many of the
nanomaterials look promising; however research efforts must be focused on the final aim
i.e. to create cost-efficient devices for rapid in-field pesticide detection/screening in
polluted areas.
Bioterrorism - linked diseases are mostly rare or eradicated infections that are sought to
be unleashed on unsuspecting civilian human society with the intent of criminal harm.
The threat of bioterrorism was long ignored until it cast its ominous shadow in recent
times through events in the United States, Japan, Iraq and Russia. It was not until the
2001 anthrax attack in the U.S. that the world started to view bioterrorism as a potential
public health threat. Bioterrorism can pose threat in domestic and international lowintensity conflicts (LICs) too.
Detection and identification of bio-threat agents include biosensing strategies based on
capturing the genetic / molecular signatures of various BWAs or their toxic metabolites /
toxins. Suitably developed biosensors can effectively screen air, water, soil, food, fresh
and waste plant and animal agro material for mitigation of bio-threat paradigm in
newly unfolding Defence scenarios as future regimes of war are more likely to fall in the
realm of unconventional than conventional technological armed conflicts / warfare.
Biosensors are also endowed with the ability to detect, analyse and quantify
biomolecules of different origins and provide information on the quality of food or fresh
agriculture / horticultural material in terms of their pesticide, fertilizer and dioxins
residue loads. Biosensors can also provide invaluable information on the state of
contaminated water and soil residues, genetically modified organisms (GMOs),
pathogenic microbes and their toxins, food anti nutrients, allergens, drug residues,
additives and hydrocarbon residues. Role of biosensors can also be viewed useful in
evaluating food composition that undergo transformation during post-harvest handling,
processing and storage as the value added food traverses through the food / cold chain.
Biosensors can real - time monitor food quality change variables such as pH,
temperature, pressure, biological metabolites, chemical or biological toxins, flow and
volatile substances. In the food industry, biosensors can play vital role in the
implementation of HACCP plans by detection of food borne pathogens and chemical
contaminants. Biosensors hold promise as alternative to conventional laboratory
analytical tools since they are intertwined with advantages in terms of size, cost,
specificity, rapid response, precision and sensitivity. In the changing Defence paradigm
of bio-threat, the need of the hour is to design and develop high precision biosensors
that are capable of being operated at different scales of operation and under varying eco
systems, such as a hand held biosensing device, a biosensor capable of being mounted on
a UAV (unidentified air vehicle), UGV (unidentified ground vehicle) and / or UUWV
(unidentified under-water vehicle).
SP-030
EXTENSION AND ADOPTION OF

NANOBIOTECHNOLOGY BY FARMERS
Santosh Rani *and Makhan Lal1
1
Deptt of Extension Education and Communication Management, Deptt of Vegetable Science

CCS Haryana Agricultural University-Hisar-125004 (Haryana)
Email: santoshpanwar78@gmail.com
Nano technology as a new and emerging technology along with the biotechnology and
information technology will undoubtedly be cover a spectrum of issues related to
agriculture sector. Nanotechnology can play an important role in improving the quality and
quantity of agricultural products. Therefore, it is necessary to remove the impediments
faced by farmers and provide basic information to enable the spread of nanotechnology.
The adoption of any new technology and innovations has not been an easy task and it is
usually not spontaneous, the technology has to be taught and learnedadopted to existing
experience and integrated into production. There are not all the farmers adopted
technologies introduced because it they are new to them. They were feeling hesitate in
adoption of new technolgies because they do not believe that in new technologies can
ensure the high yield. These farmers usualy old age and work based on their experiences.
Though farmers perceived technology as good thing to them, they still faced problems in
application of technologies. These comprises of lacking of capital, direction of
government, and extension, lack ensure of yield by compensation policies. Successful
adoption of any new technology in developing countries will depend on the availability
of technologies appropriate for local agricultural conditions, and policies that enhance the
ability of poor farmers to obtain these technologies. Evidence shows that even small
efforts to informing farmers and increasing their knowledge about the new technologies
can have big results. Therefore, it is necessary to remove the impediments faced by rural
population and provide basic information in rural areas to enable the spread of new
technologies. There is need for more trainings and educations to change the attitude of
farmers and enhance their confidence about the role of nanotechnology in agriculture. It is
also important to develop policies that benefit small-scale farmers and attend their
technological needs. Several parameters have been identified as influencing the adoption
behavior of farmers and social scientists investigating farmers who adopt the
biotechnology showing the demographic variables, technology characteristics, information
source, knowledge, awareness, attitude and group influence affect adoption behavior.
SP-031
APPLICATION OF NANOTECHNOLOGY IN
MEDICAL TEXTILES
Sarita Devi, Neha Chauhan and Nisha Arya
Department of Textile and Apparel Designing, I.C. College of Home Science, CCSHAU, Hisar
Email: chauhan.neha.5492@gmail.com
Nanotechnology related textiles can play an important role in the medical sector. The use
of nanotechnology in the textile industry has increased rapidly due to its unique and
valuable properties. Nanotechnology is defined as the utilization of structures with at
least one dimension of nanometer size for the construction of materials. Nano particle
application during conventional textile processing techniques like finishing, coating and
dyeing enhances the product performance manifold and imparts hitherto unachieved
functionality. Woven and nonwoven Anti-bacterial fabrics can be used to prevent
infection or deodorize medical clothing, wound dressing, and bedding. Fabrics can be
functionalized at the surface for tissue engineering, drug delivery or topic treatments,
such as the use of chitin for wound healing. Non-woven nano fiber cloths and filters can
find application in a variety of medical equipment, from respiratory equipment to
transfusion/dialysis machine. Nano-Tex improves the water-repellent property of fabric
by creating Nano-whiskers and nano-sized titanium dioxide and zinc oxide were more
efficient at absorbing and scattering UV radiation than the conventional size, and were
better able to block UV.
The present article is on the applications of nanotechnology in medical textiles. Types of
medical textiles and nanotechnology used for development of products related to
medical textiles such as antibacterial, uv resistant, water resistant others like nano
matrix, nano coating, nan composites, plasma assisted nano coating and polymeric nano
fibers also be discussed.
AEROGEL IN TEXTILES
SP-032
Sarita Devi, 2Saroj.S.Jeet Singh
1
1
Department of Textile and Apparel Designing, 2Department of Textile and Apparel Designing
COHS, CCS HAU, Hisar-125 004, Haryana
Email: saritahooda1991@gmail.com
Aerogels are highly porous materials with extreme properties. It was in 1930 that Kistler
invented the first process for making such a highly porous inorganic silica product.
(Venkaterman et al.2015).Aerogels are also known as frozen smoke, solid smoke, solid
air, or blue smoke. It consists of more than 96% air. The remaining 4% is a wispy matrix
of silicon dioxide. Its chemical structure similar to glass. Aerogel are mainly three types
i.e. silica, carbon and metal oxides. silica aerogel used for insulation , carbon aerogels
useful for super capacitors, fuel cells and desalination systems, metal oxides aerogel are
also used in the production of explosives and carbon nanotubes. Solgel process and
supercritical drying method is used for making aerogels. It can be used for the
development of a wide varieties of products in field of textile arogel used in
antibacterial textiles, medical textile, insulating textiles, fire retardant textiles, intelligent
textiles, clothing & apparels, footwear , gloves, green building and other thermal
insulating products also discussed in this article .The unique properties of aerogels are
extremely useful for designing flexible and lightweight insulation materials. Aerogel is
an amazing insulator; it has to be since NASA uses it in outer space where the
temperature is only 2 degrees above absolute zero.( Stella, 2016). The mechanical and
thermal properties of the product may be varied based upon the choice of reinforcing
fibres, the aerogel matrix, and pacifications additives included in the composite. Silica
aerogel blankets made from silica aerogel integrated into nonwoven fabrics and reduce
energy consumption from heating or cooling the interior of a fabric structure by 30%
70%, depending on the climate and is being used as the insulation, in the field of energy
harvesting carpets which can convert the stresses acting upon it when people walk over
it, in to usable form of energy to power a small battery and many more. Benefits of
application of aerogel product i.e. labour costs and maintenance costs will be decreased
due to self-cleaning super hydrophobic surface of the elements and improved human
comfort due to moisture management.
SP-033
NANOTECHNOLOGY: APPLICATIONS IN YIELD

ENHANCEMENT
Seema Kumari, Bhawna Batra, Namita, C.S Pundir
Department of Biochemistry, M. D. University, Rohtak (India)
Email: kumarieseema2071@gmail.com
Nanotechnology is science of manipulating materials at nano-scale. Among the latest

technological advancements, nanotechnology occupies a central position. It has many
applications in all stages of production, processing, storing, packaging and transport of
agricultural products.. In a nanotech research initiative in Thailand that aims to atomically
modify the characteristics of local rice varieties drilled a hole through the membrane of a
rice cell in order to insert a nitrogen atom that would stimulate the rearrangement of the
rice DNA to alter the colour of a local rice variety from purple to green. The application of
semiconducting nanoparticles to live plant cells in culture. Utilizing this technique, it is
very clearly explained and showed the position of plant pollen tube adhesion protein along
with stigma/stylar cysteine-rich adhesin (SCA) region. This has potential to trace out self
incompability barrier in crop plants. Nanotechnology, though, has positive impact on crop
improvement by use of various techniques, yet some negative reports are also available.
One of the examples has been reported in rice. The toxic effect of multi-walled carbon
nanotubes (MWCNTs) on plant cells and observed increased of reactive oxygen species
(ROS) and decreased of cell viability, however when a primary antioxidant, ascorbic acid
was added to the rice cell suspension, it showed a positive effect by decreasing reactive
oxygen species (ROS) and increase cell viability. In conclusion it can be very safely stated
that the appropriate and timely use of nanotechnology has vast opportunities in the area of
crop improvement, But at the same time application of nano-materials in agro-food sector
has to be evaluated for public acceptance so it does not come across a scenario as faced by
GMOs in past.
SP-034
APPLICATION OF NANOTECHNOLOGY FOR THE

ENCAPSULATION OF BOTANICAL INSECTICIDES FOR
SUSTAINABLE AGRICULTURE
Shalini Pandey, Sankara Rao, K.
Department of Entomology, College of Agriculture, CCSHAU, Hisar
Email: pandeyshalini80@gmail.com
Sustainable food production for a rapidly growing human population is one of the major
challenges faced by the agriculture sector globally. Insect/Pests are the major limiting
factor in profitable crop production. Indiscriminate use of pesticides has led to many ill
effects to both biotic and abiotic components of environment. Therefore, the use of
botanical insecticides associated with nanotechnology offers considerable potential for
increasing agricultural productivity, while at the same time reducing impacts on the
environment and human health. Nanoparticles are biodegradable and can be successfully
employed in production of nanocapsules for delivery of pesticides. Nanoparticles display
slow release of encapsulated functional molecules and reduce its frequent
applications.Derivatives of neemAzadirachta indica A. Juss are widely used in agriculture
to combat insects and other pests. Riyajan and Sakdapipanich (2009) developed capsules
of sodium alginate reticulated with glutaraldehyde and coated with natural rubber.
Microcapsules coated with rubber providing slower release (80%) of azadirachtin (active
compound) compared to uncoated capsules (100%) in same release period.Da Costa et
al(2014)prepared different types of formulations containing azadirachtin and tested against
the bean weevil Zabrotessubfasciatus(Bohemann).Commercial product was completely
degraded by UV radiation within seven days, while the encapsulated azadirachtindegraded
only 20% after 14 days.Yang et al (2009)investigated efficacy of polyethylene glycol
nanoparticles, carrier for the essential oil ofgarlic Allium sativum L.against red flour beetle
Triboliumcastaneum (Herbst). Formulation showed 80% effectiveness against adult beetles
after five monthswhile essential oil of garlic showed 11% effectiveness.Thus,
nanotechnology will revolutionize agriculture including pest management in the near
future.
SP-035
NANOTECHNOLOGY IN PLANT DISEASE

MANAGEMENT
Shipra Gangopadhyay and Sudhir Sharma
Department of Genetics and Plant Breeding, College of Agriculture
CCS Haryana Agricultural University, Hisar, Haryana
Email: shipragangopadhyay@gmail.com
Nanotechnology is a multidisciplinary field with enormous potential to boost agricultural

research. Recently science of materials at the nanometer scales has emerged as one of the
most promising subject. Novel materials and surface characteristics can be utilized to
enhance agricultural productivity and production. Nanomaterials can be potentially used
in crop protection and disease management. Nanomaterials can be used as carrier of
host-defence inducing chemicals,active ingredients of pesticides, to target pathogens.
Nanoparticles may target virus particles because of their ultra small size and can open a
new field of virus control in plants. With the use of nanosensors the disease diagnosis,
pathogen detection and residual analysis may become much more precise and quick.
Bacterial spot caused by Xanthomonas perforans is a major disease of tomatoes, leading
to reduction in production by 10-50%. It has been found that DNA-directed silver
nanoparticles grown on grapheme oxide at very low concentration of 16 ppm can
effectively decrease bacterial cell viability in culture and on crop plants. The composites
show excellent antibacterial capability in culture with significant advantages in improved
stability, enhanced antibacterial activity and stronger adsorption properties. Silver
nanoparticles are also found to inhibit the colonization of Staphylococcus aureus,
Psedomonas aeruginosa, Escherichia coli and Klebsiella pneumonia. Highest
antimicrobial activity of silver nanoparticles (30mm) synthesized by Solanum tricobatum
and Ocimum tenuiflorum leaf extracts has been observed against S. aureus and E. coli.
The nanoparticles showed strong antifungal effects against Candida albicans, C.
glabrata and Aspergillus brasiliensis. Fungicidal effect of zinc oxide nanoparticles
against Botrytis cinerea and Penicillium expansum has also been reported.
SP-036
NANOPARTICLES IN ABIOTIC STRESS

Sonali Sangwan and Shikha Yashveer
CCS Haryana Agricultural University Hisar
Email: sonalisangwan03@gmail.com
Abiotic stresses such as drought, salinity, submergence etc. are considered as the major
factors that contribute to major reduction in crop yield. Nanotechnology is fast growing
technology with latest updates and advancement in diverse sectors. There are several
studies which indicate that nanoparticles can be used to help plant combat stress.
Nanoparticles are involved in upregulating the activities of antioxidant enzymes like
SOD, CAT and POD in a concentration dependent manner. Studies have suggested that
TiO2 and SiO2 nanoparticles have potential to enhance seed germination as well as
growth of seedlings such as in glycine max, onion, lentil etc. It has been reported that
silicon nanoparticles and silicon fertilizer induced tolerance in basil by significantly
increasing growth and development indices, chlorophyll content (Chl a) and proline
level. Silicon can be potentially used to mitigate effects of drought stress impact up to
some extent as suggested by a study. Although the exact mechanism is unclear but
silicon partially improves shoot growth, increase the leaf chlorophyll contents, maintains
leaf water potential in stressed plants. Moreover, it also reduces membrane lipid
peroxidation in wheat. Iron nanoparticles also exhibits drought stress mitigating effects
on yield components and oil percentage as observed in Goldasht spring safflower
cultivars. As the applications of nanotechnology and use of nanoparticles in sustainable
agriculture and crop improvement are still at juvenile phase it is necessary to build up
basic understanding regarding interaction of nanoparticles with plants at cellular as well
as molecular level.
SP-037
NANOBOTMODEL: GOAL OF FUTURE

NANOMEDICINE
Sudesh Rani
Department of Zoology, M.D.University, Rohtak
Email: sudesh_zoology@rediffmail.com
Nanomedicine is the process of diagnosing, treating, preventing disease, relieving pain of

traumatic injury and improving human health by using molecular tools and molecular
knowledge of the human body. In the relatively near term, nanomedicine can address many
important medical problems by using nanoscale-structured materials and simple
nanodevices that can be manufactured today, including the interaction of nanostructured
materials with biological systems. In the mid-term, biotechnology will make possible even
more remarkable advances in molecular medicine including microbiological biorobots or
engineered organisms. A nanorobot is a tiny machine designed to perform a specific task
or tasks repeatedly and with precision at nanoscale dimensions i.e. dimensions of a
few nanometers (nm) or less, where 1 nm = 10-9 meter. Nanorobots have potential
applications in the assembly and maintenance of sophisticated systems. Nanorobots might
function at the atomic or molecular level to build devices, machines, or circuits, a process
known as molecular manufacturing. In the longer term, perhaps 1020 years from today,
the earliest molecular machine systems and nanorobots may join the medical
armamentarium, finally giving physicians the most potent tools imaginable to conquer
human disease, ill-health, and aging.
SP-038
NANOTECHNOLOGY, AN EFFICIENT
ALTERNATIVE IN INSECT PEST MANAGEMENT
Suchismita Balabantaray, Dr. R.S. Jaglan
Department of Entomology, CCS Haryana Agricultural University, Hisar, Haryana
Email: suchi.balabantaray26@gmail.com
Nanotechnology is a promising field of interdisciplinary research. It opens up a wide array

of opportunities in various fields like medicine, pharmaceuticals, electronics and
agriculture. The potential uses and benefits of nanotechnology includes insect pests
management through the formulations of nanomaterials-based pesticides and insecticides,
nanoparticle-mediated gene or DNA transfer in plants for the development of insect pestresistant varieties and use of nanomaterials for preparation of different kind of biosensors,
which would be useful in remote sensing devices required for precision farming.
Traditional strategies like integrated pest management used in agriculture are insufficient,
and application of chemical pesticides like DDT have adverse effects on animals and
human beings apart from the decline in soil fertility. Therefore, nanotechnology would
provide green and efficient alternatives for the management of insect pests in agriculture
without harming the nature. It is now known that many insects possess ferromagnetic
materials in the head, thorax and abdomen, which act as geomagnetic sensors.
Microelectronics and nanotechnology have been combined to create a tiny sensor that can
help farmers in the early detection of grain spoilage during storage. The stand-alone sensor
is the size of a dime and can detect parts per billion levels of carbon dioxide and odour
causing chemicals to determine the level and cause of spoilage.
SP-039
ROLE OF NANOTECHNOLOGY IN FOOD

INDUSTRY
Sukhdeep Singh Sivia, Anil kumar, Sunayana and Reena Rani
Department of Genetics & Plant Breeding, CCS Haryana Agricultural University, Hisar
Email: sukhdeepsinghsivia@gmail.com
New concepts and engineering approaches involved in functional foods and nutraceuticals
usingnanomaterials to target the delivery of bioactive compounds and micronutrients is
developing. Nanomaterials allow better encapsulation in liposomes, micelles,
nanoemulsions, cubosomes and release efficiency of the active food ingredients as
compared to traditional encapsulating agents. Nanomaterials are also quite stable and can
be processed cost effectively. Nano-emulsions can encapsulate functional ingredients
within their droplets which can facilitate a reduction in chemical degradation.
Nanolamination is a technique for protecting the food from moisture, lipids and gases.
Moreover, they can improve the texture and preserve colour and odour of the food.
Nanolaminates consist of two or more layers of nano-sizedthin foodgrade films which are
present on a wide variety of foods like fruits, vegetables, meats, etc. Coating foods with
nanolaminates is done simply by spraying it on the food surface.Antimicrobial packaging
of edible food films made with cinnamon or oregano oil, or nanoparticles of zinc, calcium
other materials that kill bacteria is being tried. Green packaging using nano-fibers
madefrom lobster shells or organic corn is also a food safetyeffort. Improved food
packaging needs packagingmaterials having strength, barrier properties and stability to
heat and cold which can be achieved using nanocomposite materialsenriched with silicate
nanoparticleswhich reduces the entrance of oxygen and other gases, and preserves
moisture, thus preventing foodfrom spoiling.A nanocomposite film enriched with silicate
nanoparticles reduces the entrance of oxygen and other gases, and preserves moisture, thus
prevents food from spoiling.
SOLAR CELLS, NANOFIBRES, SENSORS,
ULTRA LIGHT MATERIALS AND CORROSION PREVENTION
SP-040
Suman and Vinod Kumar Jain

Research Scholar, Department of Veterinary Medicine, College of Veterinary Sciences,
LalaLajpatRai University of Veterinary and Animal Sciences (LUVAS), Hisar (Haryana)
Email: raveshsuman24@gmail.com
Materials scientists and engineers have made significant developments in the

improvement of methods of synthesis of nanomaterial solids. Unprecedented
opportunities are arising for re-engineering existing products. The buckyball (C60) has
opened up an excellent field of chemistry and material science with many exciting
applications because of its ability to accept electrons. Nanoporous oxide films such as
TiO2 are being used to enhance photo voltaic cell technology. Nanoparticles are perfect
to absorb solar energy and they can be used in very thin layers on conventional metals to
absorb incident solar energy. Production of nanofibres offers the potential of using the
woven reinforcement as body armor. Nanotechnology offers unlimited opportunities to
produce new generation pressure, chemical, magneto resistive and anti-collision
automobile sensors. Many of the novel applications such as new sensors, better
photovoltaic cells, lighter and strong materials for defense, aerospace and automotives
are used as sensors. It is a key technology for the development of ultra light materials
which would result in energy, fuel and materials savings and development of spectacular
materials with complete control over structure and properties at a subatomic level. A
typical feature of nanomaterials is the defect core structure, which is caused by
incorporation of vacancies, dislocations, grains or interphase boundaries, which alter the
density and conduction in defect core regions. Nanoparticles incorporated in coatings
have shown a dramatic resistance to corrosion of the substrate due to their hydrophilic,
anti-wear, anti-friction and self-cleaning properties. Nanocoatings create a lotus effect
and properties, which keeps corrosion away.
NANOTECHNOLOGY- AN ADVANCE AND

EFFICIENT SUBATOMIC LEVEL TECHNIQUE
FOR TREATING DETERIORATED SOIL AND BIOREMEDIATION
SP-041
Suman Chaudhary, Rinku Dhanker, Tanvi and Sneh Goyal

Departement of microbiology, CCS Haryana Agricultural University, Hisar-125004
Email: kesarsuman21@gmail.com
As we know our soil health is degrading day by day due to continuous use of chemical
fertilizers and overproduction. So to feed the vigorously expanding population size, we
have to take care of it. Nanotechnology is an incipient field of science which may be
defined as technique involving nanoparticles having dimension of 100 nm or less in at
least one dimension and their effectiveness can be nationalized from size itself with which
these work. A nanometer (nm) is a billion part of meter, i.e., 109 m. There are so many
approaches have been reported of using nanomaterials for cleaning water and
contaminated soils. Most commonly used nanoparticle is zero valent (Fe0) iron
particle.These have been experimented as adsorbents or catalysts to remove toxic and
harmful substances from air and wastewater and then finally from affected soil. They can
be transported easily with the groundwater flow and besides their minute size, they may
face effectively the challenges of site remediation. A new class of iron phosphate
(vivianite) nanoparticles have been prepared and tested for in situ immobilization of Pb+2
in soils by reducing their leachability and bio accessibility from soils. Multi-walled carbon
nanotubes (CNTs) were also used successfully for the removal of Copper (II), Lead (II),
Cadmium (II), and Zinc (II) from aqueous solution. The application of nanotechnology
may prove promising in the future and it can provide an accessible way to purify the soil,
water and air by utilizing nanomaterials as a catalyst and/or sensing systems.
SP-042
NANO TEXTILES: ARISING TRENDS IN SPORTS

Suman Sodhi and Nisha Arya
Department of Textile and Apparel Designing, I.C.COHS, CCSHAU, Hisar, Haryana
Email: sumansodhi25@gmail.com
Nano-textiles is an emerging and interesting application of nanotechnology. The

impact of nanotechnology on sports is becoming more and more visible. It involves
dealing with nano fibers at the atomic and molecular levels in order to twist their
properties. This novel technology can give rise to incredible clothing such as waterresistant and dirt-free clothes, odor-less socks and intelligent clothes that can perform
climate control for people. Nanotechnology may also play a role in sports such as
soccer, football, baseball, etc. Materials for new athletic shoes may be made in order to
make the shoe lighter. Baseball bats already on the market are made with carbon nanotubes
which reinforce the resin, which is improve its performance by making it lighter. Nano
additives are utilizes to enhance the surface characteristics of clothes such as water resistance, UV-protection, wrinkle resistance, color durability, flame retardancy and better
thermal performance. Silver nanoparticles are antimicrobial in nature and are now widely
used in sports clothing to eliminate unpleasant odors, they also create some risks to the
user and the environment. Nanotechnology has even found applications in the wide field of
sports. Within the niche of sports, nanotechnology has proven to very useful, and has the
potential to improve a broad range of aspects of the sports world. The application of
nanotechnology in various spheres of human life has caused its rapid development in
recent years. Nanotechnology has also proven its potential even in the field of sports.
SP-043
NANOTECHNOLOGY FOR GROWTH AND

DEVELOPMENT IN PLANTS
Sumit Jangra, Asha Rani, Rekha Patel, Disha & R.C. Yadav
Department of Molecular Biology, Biotechnology & Bioinformatics
CCS HAU, Hissar
Email: sumit.jangra712@gmail.com
Nanotechnology has emerged as one of the most innovative scientific field in agriculture.
It has been applied to biological science fields since the 1990s and currently
nanomaterials, the most advanced level of nanotechnology, are being employed in crop
research. These include fluorescent labels, gene delivery, bio detection of pathogens,
detection of proteins probing of DNA, bio-imaging and separation and purification of
biological molecules and cells. Magnetic nanoparticles have received enormous attention
because they allow specific localization of the particles to release their load, which plays a
crucial role in the applications of nanoparticulate delivery for plants. Researchers have
reported the uptake, translocation and specific localization of magnetic nanoparticles in
pumpkin plants. No toxicity was detected on plant growth which suggested that these
kinds of nanoparticles are safe for nanoparticulate delivery in plants. Scientists reported
that in an aqueous medium containing magnetite nanoparticles for the growth of Cucurbita
maxima, particles can absorb, move and accumulate in the plant tissues. On the other hand,
Phaseoluslimensis is not able to absorb and move particles. Therefore, plants have
different response to the same nanoparticles. This fast movement of the nanoparticles
inside the plants can be an important factor in the development of nanoparticles as smart
delivery systems inside the plants. Application of nanotechnology in agriculture, even at
its global level, is at its nascent stage. Nanoscience is leading to the development of a
range of inexpensive nanotech applications for enhanced plant growth.
SP-044
APPLICATIONS OF NANOTECHNOLOGY IN
AGRICULTURE
Sunayana, Reena Rani, Sukhdeep Singh Sivia andAnil kumar
Department of Genetics & Plant Breeding
CCS Haryana Agricultural University, Hisar, Haryana (India), 125004
Email: nainapunia@gmail.com
Nanotechnology encompasses the production, characterization and application of

materials with dimensions measured at nanometer scale (10-9), typically less than 100
nm. Enhanced properties exhibited by nano-sized particles and materials enable
widespread potential applications, increased focus on agricultural input use efficiency
and reducing impact to the environment.The application of nano-technology include
disease control, slow release of pesticides and developing diagnostic tools, development
of functional food systems, to produce interactive, edible nano wrappers to keep the
pathogens away, targeted release of chemicals, packaging, extensive nano surveillance
and interactive agrochemicals as herbicides and pesticides. Nanotechnology has provided
new solutions to problems in plants, plant products and post-harvest technology in
enhancing the quality of plant products. Nano-fertilizers (Nano-5and NanoGro)for
efficient use and slow release of nutrients as plant growth regulatorsare
used.Nanopesticidesare pesticides encapsulated in nanoparticles for controlled
release,nano-emulsions for greater efficacy while nanomaterial for soil and water
conservation. Nanosensors are used for precision agriculture, pathogen and contaminant
detection. Nano celluloseis produced from agricultural residues. However regulatory
guidelines are still evolving and no standardized protocols for toxicity testing and
evaluating environmental impact of nanomaterials are there. Long gestation periods and
cost of equipment pose challenge for sustained funding for nanotechnology research and
commercialization efforts. Scale of operations in agriculture pose a different set of
challenges when compared to nanotechnology applications in other industries. Use of
nanotechnologycould permit rapid advances in agricultural research, such as
reproductive science and technology,early detection of stresses and alleviating stress
effects.
SP-045
BIOTECHNOLOGICAL ASPECTS OF JOJOBA

Sunil Kumar1 and Narender Singh2
1
Department of Botany, CRM Jat PG College, Hisar

Department of Botany, Kurukshetra University, Kurukshetra
*Email: sunyad84@gmail.com
Simmondsia chinensis commonly known as jojoba (pronounced as ho-ho-ba) is the

sole species in the family simmondsiaceae and is native to Sonoran desert of South
Western USA and Northern Mexico. It is an economically important wind pollinated,
evergreen, perennial dioecious shrub, reaching a height of 3-5 meters with leathery,
greyish green leaves. It is a drought resistant crop which thrives well under unfavourable
soil conditions due to its deep root system. Jojoba can be grown in all types of soils
except heavy soils. Its pH requirement ranges from 5-8 indicating its tolerance to acidic
as well as alkaline conditions. It can also tolerate extreme temperatures ranging from -5
to 54oC and hence can be grown on marginal lands that are not used for conventional
agricultural crops. In India, it was introduced in 1965 at Central Arid Zone Research
Institute, Jodhpur, from Israel.
The seeds of this plant contain a light-gold coloured wax ester (commonly known as
jojoba oil) that makes up 50-55% of the seed weight. The oil contains only traces of
saturated wax, steroids, tocopherol and has no resins and tars. During the past few years,
jojoba has become the focus of attention all over the world as hunting of whales (now
endangered) has been banned and jojoba oil has almost the same properties as the oil
obtained from the sperm whale. The liquid wax and its derivatives have potential in a
wide range of applications in cosmetics (lipsticks, face creams, skin fresheners, winter
care lotions, shampoos, moisturizers, soaps), lubricants, anti-foaming agents,
pharmaceutical (antibiotic production, coating of tablets, to treat skin disorders, sores,
wound, burnt skin and to remove stretch marks), electrical insulators and plastic
industries. It has no cholesterol or triglycerides and therefore can be used as low calorie
edible oil. Indigenous Americans and Indians used jojoba seed and oil for cooking, hair
care and for medicinal treatments such as poison ivy, sores, wounds, colds, cancer and
kidney malfunction. It can also be used as an alternate fuel oil with fewer pollutants and
exhaust is free of harmful SO2. Owning to all above properties, jojoba oil is claimed as
one of the natures gift to human race or liquid gold from the desert. Efforts have been
made for micropropagation, biochemical and genetic fidelity studies on jojoba.
SP-046
SMART TEXTILES: A MODERN GLIMPSE IN

TEXTILE WORLD
Sushma Rani, Zeba Jamal, Parveen Punia and Vivek Singh
Department of Textile and Apparel Designing2
COHS, CCS HAU, Hisar-125004, Haryana
Email: kantiwal20shushma@gmail.com
Todays aesthetic aspects came to play a role in clothing. Textile materials are of interest
to everyone, which play a vital role in life. Much more recently a new generation of
textiles has arisen i.e. smart textiles. It is defined as textiles that can sense and react to
environmental conditions or stimuli from mechanical, thermal, chemical, electrical or
magnetic sources. Smart clothing that makes us feel comfortable at all times, during any
activity and in any environmental conditions that protects and even helps to treat
diseases and injuries. It has enabled manufacture of textiles with special functionalities.
These smart or natural textile products have excellent market potential both India and
abroad. Smart and natural textiles have enormous potential to diversify textile
manufacturing with extensive applications in sports, medical fields, personal wear,
condition monitoring, food etc. Antimicrobial textiles also improve functionality of
textile articles, especially garments, worn close to the skin and also in several medical
applications, such as infection control and barrier material. Moreover extract from plant
source with active substances are eco-friendly, non-toxic and non-allergic. Peach leaves
extract on cotton fabrics is an effective treatment for bacterial resistance or develop a
smart or natural textile. Serviceability of smart textiles in various spheres of human life
has rapidly expansion in recent years.
SP-047
ROLE OF NANOTECHNOLOGY IN
SMART TEXTILES
Sushma Rani, Zeba Jamal, Parveen Punia and Vivek Singh
Department of Textile and Apparel Designing, COHS, CCS HAU, Hisar-125004, Haryana
Email: kantiwal20shushma@gmail.com
Nanotechnology holds an enormously promising future for Electronic textiles (Etextiles). It is the science, engineering and technology conducted at the nanoscale and an
application of extremely small things and used across all the other fields such as textiles,
e-textiles, cosmetics, agriculture, medical science, engineering etc. Smart textiles are
fabrics that have been designed and manufactured to include technologies that provide
the wearer with increased functionality. These textiles have numerous potential
applications, such as the ability to communicate with other devices, conduct energy,
transform into other materials and protect the wearer from environmental hazards. Smart
textiles allow the design and production of a new generation of garments with distributed
sensors and electronic functions. Smart textiles are fabrics that have been developed
with new technologies that provide added value to the wearer.
A first generation of nano-enhanced textiles benefitted from nano finishing:
Coating the surface of textiles and clothing with nanoparticles is an approach to the
production of highly active surfaces to have UV-blocking, antimicrobial, antistatic,
flame retardant, water and oil repellent, wrinkle resistant and self-cleaning properties. In
smart textiles fabrics can be efficiently produced for different purposes including
medical, military, industrial apparels, flexible body armour, radio shielding and
camouflage etc. Nanotechnology overcomes the limitations of applying conventional
methods to impart certain properties to textile materials. In the next few years smart
textiles will penetrate into every area of textile industry.
SP-048
BIOLOGIAL SYNTHESIS OF NANOPARTICLES

USING ZINC SOLUBILIZING MICROORGANISMS
Swati, Sunita Suneja, Subha and Rajesh Gera
Department of Microbiology, CCS Haryana Agricultural University Hisar-125004
Email: swati3357@gmail.com
Nanotechnology has become one of the most important technologies in all areas of
science. It relies on the synthesis and modulation of nanoparticles, which require
significant modifications of the properties of meta zinc and titanium nanoparticles have
been used in biomedical, cosmetic, ultraviolet (UV)-blocking agents, and various cuttingedge processing applications. Microorganisms have been shown to be important
nanofactories that hold immense potential as ecofriendly and cost-effective tools, avoiding
toxic, harsh chemicals and the high energy demand required for physiochemical synthesis.
Microorganisms have the ability to accumulate and detoxify heavy metals due to various
reductase enzymes, which are able to reduce metal salts to metal nanoparticles with a
narrow size. Zinc solubilizing bacteria isolated from seven rhizospheric soil samples of
different crops were screened for the property of solubilization of insoluble zinc sources.
Zinc solubilizing ability was assessed using zinc oxide, zinc sulphide and zinc carbonate in
plate assay. All the isolates solubilized zinc oxide with solubilization index ranging from
1.56-36.00; three isolates solubilized zinc sulphide with index 1.96-4.00 and thirty three
isolates solubilized zinc carbonate with index 3.36-25.00. Bacterial isolates (MR1, CR2
and OR1) tested for zinc tolerance level, in isolate MR1, significant reduction in
population was observed at 200-500 mg l-1 of zinc sulphate OR1 showed highest zinc
tolerance level. These bacterial isolates can be exploited for the synthesis of zinc oxide
nanoparticles, which will not only increase the Zn solubilzation acivity but also protect the
environmental pollution.
SP-049
SOIL MINERAL STATUS AND ITS EFFECT ON

GLYCOLYTIC PATHWAY IN RICE (Oryza sativa L.)
Sweety Sihag1, Ritu Saini1 and Sunita Jain2
Department of Chemistry and Biochemistry, 2Department of Molecular Biology, Biotechnology and

Bioinformatics, CCS HAU, Hisar, India
Email: sweetypoonia2011@gmail.com
Rice (Oryza sativa L.) is the commonly consumed crop but contains low levels of the
key micronutrients iron (Fe), zinc (Zn). Iron and zinc malnutrition is the most common
deficiency that has affected the people world over. Biofortification of rice grains with Fe
and Zn seems to be the most suitable strategy to combat malnutrition. However, Fe and
Zn even though essential to plants are toxic in excess, and their accumulation is tightly
regulated. To understand the effect of Fe and Zn on metabolism, efforts were made to
study the glycolytic pathway in four rice varieties viz. HBC19, HKR47, PUSA1121 and
PAU201 grown in pots and seven different concentrations of Fe(II)-EDTA+ ZnSO4 were
supplied through Yoshida nutrient medium. All the varieties showed decline in the
activities of key glycolytic enzymes; Hexokinase (HK), Phosphofructokinase-1(PFK-1)
and Pyruvate kinase (PK) while increase in activity of Fructose-1,6-bisphosphate
aldolase (FBP- aldolase) was observed under low iron and high zinc (0.05mM Fe(II)EDTA + 0.48 mM ZnSO4.7H2O) treatment. Contrarily, at high iron and low zinc (0.5
mM Fe(II)-EDTA + 0.06 mM ZnSO4.7H2O ) treatment, activities of HK, PFK-1,and PK
increased while the activity decreased in FBP-aldolase. Differential activities of HK,
PFK-1, FBP-aldolase and PK were noticed at various concentrations of iron and zinc
treatments. Novel developments in nutrient uptake mechanisms using nano fertilizers
containing essential micronutrient like zinc and iron is an important area of research to
boost productivity and biofortication in crops like rice.
SP-050
ROLE OF NONMATERIAL IN ENHANCING THE

RESISTANCE OF PLANTS TO ABIOTIC STRESSES
Kirpa Ram, Sunita, Pooja and Renu Munjal*
Department of Botany and Plant Physiology, *Department of Genetics and Plant Breeding
CCS Haryana Agricultural University, Hisar-125004
Email: dr.kirparamjangra@gmail.com
Crop plants are adversely affected by abiotic stresses. At the global level, drought high
temperature and salinity are most widespread and commonly experienced stresses that
profoundly affect yields of cultivated crops. Also, the level of abiotic stresses is on the rise
due to both natural and man-made interventions, scientific community has a major concern
to mitigate their effect in order to increase the yield potential of crops. Therefore,
nanotechnology a new source for solutions to several agricultural and environmental
challenges, such as sustainability, improved varieties and increased productivity. Today, a
numbers of nanoparticle are used in agricultural crops for abiotic stress management such
as: Au, Ag, Pd etc (metal) or ZnO, CeO2, Fe2O3, Al2O3 etc (metal oxide). Fe2O3 NPs in
Cereal crops (Rice and Wheat) increased root length, plant height, biomass, SPAD
chlorophyll values and yield in plants under ionic stress and other heavy metal toxicities.
ZnO NPs increases the auxin (IAA) level in roots of germinating seedlings and overall
biomass production of plants. ZnO NPs, Fe2O3 NPs are used also as a foliar fertilizer to
mitigate the ionic toxicity. CeO2 NPs enhances antioxidant stress tolerance by y
modulating various processes like lowering of superoxide radicals accumulation, hydrogen
peroxide, malonyldialdehyde content, and inducing antioxidant enzymes activities under
water deficit condition. Therefore, nanotechnology is the promising science to yield
improvement under adverse condition. The practical use of nanomaterial in field reduced
negative impact on natural environment and crops as well.
EFFECTS OF SPERMINENANO CARRIERS AND ITS

BIOCHEMICAL IMPLICATIONS TO MITIGATE THE
DELETERIOUS EFFECT OF SALINITY IN WHEAT (Triticum
AestivumL.)
SP-051
Raghavendra Midathala1*, Shashi Madan1, Renu Munjal2 and Veena Jain1

Department of Chemistry and Biochemistry1, Department of Genetics and Plant Breeding2
CCS HAU Hisar
Email: yoursraghavendra@gmail.com
The potential effects of cellular biodegradable polycationic polyamines

(PAs)spermine(Spm) on salinity induced stress and their response were investigated in two
wheat varieties.Spermine along with other conjugated compounds such as chitosan, lignin,
phospholipids, lecithin, lactalbumin, starch, cellulose derivatives, alginates, polylactide,
poly(propylene glycol), polyacrylamide and polysorbate can act as a delivery vehicle for
nanoparticles. Salinity is the major concern of scientific community to attain sustainable
crop production, it is estimated that the saline affected soils comprise 0.63 m ha in
Haryana out of 10 m ha in India. Tolerance to salinity stress is very complex due to
intricate interactions between stress factors and various biomolecular and physiological
phenomenon affecting plant growth and development. Seeds of two wheat varieties DBW
88 and Karchia 65 differing in salinity tolerance were grown in earthen pots lined with
polyethene bags in a screen house under naturally lit conditions. The saline treated wheat
plants were sprayed with two levels of spermine concentration and the leaf samples were
analyzed for various metabolites such as proline content, ascorbate content, hydrogen
peroxide (H2O2), malondialdehyde (MDA), leaf membrane injury and osmotic potential.At
high salinity levels, proline accumulation in leaves were 3-4 folds but with spermine
treatment prolinecontent further increased. Spermine treatment reduced the excessive ROS
generation, MDA content and leaf membrane injury in both cultivars under salinity. In
particular, treatment with lower conc. (0.5mM) of spermine was found to be more
effective in mitigating the effects of salt stress in both the wheat varieties.These studies
help to understand the biochemical and physiological role of the delivery vehicle
sperminefor nanoparticle in long term salt stress induced wheat plants.
INFLUENCE OF MANUFACTURED
NANOPARTICLES ON SOIL HEALTH
SP-052
Mohammad Amin Bhat1, Inderpal Singh1, Chetan Gyawali1, Sheeraz Ahmed Wani2
1
Department of Soil Science, CCS Haryana Agricultural University, Hisar-125004

Division of Agronomy, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir,
Shalimar (J&K)-190025
Email: bhatamin8@gmail.com
Nanotechnology, as a field is emerging fast as the number and variety of materials is

increasing dramatically. However, the manufactured nanoparticles (MNPs) have put new
challenges about the potential environmental effects. There are substantial facts that
various MNPs are lethal and potentially perilous to environment. The toxicity of MNPs is
appreciably linked with their exceptional physicochemical properties like very small size
and large surface area. Presently, silver nanoparticles are most commonly commercialized
MNPs used in variety of applications, for instance, catalysis, jewelry, dentistry,
photography, medicine, cosmetics, textile and food packaging. During production,
utilization and dumping of silver nanoparticle-containing products, silver nanoparticles are
released into the environment; most of these compounds ultimately finish up in
agricultural soils via inappropriate disposal methods. These silver nanoparticles can persist
in soils or consumed by organisms, thus, can function as ecotoxicological hazards or bioaccumulate in food chain. The toxicity due to silver nanoparticles is mainly due to Ag+ ion,
however, direct interaction and uptake of nanoparticles by cells causes damage to
membranes, oxidative stress and mortality. Therefore, there is a need to mitigate the
hazardous effects of these manufactured nanoparticles by using biological organisms.
SP-053
NANOTECHNOLOGY AS AN EMERGING
APPROACH FOR SOIL AND GROUNDWATER
REMEDIATION
Kavita Rani, Geeta Bhamania
Department of Enviornment Sciences, M.D.University, Rohtak-124001
Email: Kavitamoun1986@gmail.com
Many toxic compounds and Xenobiotic from industrial activities are presently being
accumu lated in the ecosystems at higher rate. From an anthropocentric point of view such
contamination is unsustainable because it threatens basic functions of ecosystem and
ground water. These contaminated sites demand so many strategies and new technologies
which are efficient and cheap enough to make remediation effective in remote areas where
financial support is limited.. Technologies that utilize NPs for remediation of
contaminated sites have been rapidly developed in recent years because it is an emerging
technology which is capable to solve this problem. In the nanotechnology the design,
characterization, production and applications of the particles takes place by controlling
their size and shape at the nanoscale. The NMs are designed with specific properties and
can enter the environment through industrial or environmental applications and they are
used for remediation of soils and groundwater So many nanoscale materials have been
used for remediation like zeolites, nanometal oxides, nanotubes ,carbon fiber, enzymes
and bimetallic nanoparticles .But, the application of zNIP is much more for the
remediation of contaminated soils and groundwater, mainly due to its low toxicity and
low cost of production in relation to other NPs.It is found that the use of zNIP quickly
converted the Cr (VI) to Cr (III) and Pb (II) to Pb (0). So it is concluded that this NP is
suitable for use in situ remediation applications.

SP-054
NANOBIOTENCHNOLOGICAL
TOOLS FOR SOIL REMEDIATION
Ankush, Vikram Singh, Vinod Kumar

Department of Agricultural Chemistry and Soil Science, Rajasthan College of Agriculture, MPUAT,
Udaipur, Rajasthan
Email: ktankdhanda@gmail.com
Maintaining and restoring soil quality is one of the great challenges of our time. Soil and
ground water are frequently contaminated with pesticides and halogenated compounds.
Nanotechnology promises a potential revolutn in approaches to remediation. It is a broad
and indisciplinary field dealing with structure and particles at nano scale. Nanoparticles are
highly reactive and having higher sorption capacity due to high surface volume ratio. Their
small particle size also allows nanoparticles to enter small pores in soil or sediment that
larger particles might not penetrate, granting them access to contaminants sorbed to soil
and increasing the likelihood of contact with the target contaminant to immobilize it.
These materials include zero-valent metals like zero-valent iron (ZVI), calcium peroxide.
carbon nanotubes ZVI effectively reduced and dechlorinated many halogenated
hydrocarbon compounds. This degradation is mainly based on redox reactions in which
iron donated electron to the contaminants, reducing them to lesser toxic compounds. ZVI
also decrease the dissolved concentration of nitrate, perchlorate, selenite, arsenate and
chromate. Nanoscale calcium peroxide as an oxidant is also used in the remediation of
soils containing various organic contaminants, such as gasoline, heating oil, methyl tertiary
butyl ether (MTBE), ethylene glycol and solvents. Nanoscale calcium peroxide is claimed
to be highly efficient in removing aromatics and is also used in enhanced bioremediation.
The oxygen produced in the reaction of calcium peroxide with water leads to an aerobic
environment that supports natural bioremediation by aerobic organisms present in the soil.
SP-055
NANOTECHNOLOGY IN BIOREMEDIATION
Poonam Choudhary
Assistant Professor, Department of Botany
Dronacharya Government College, Gurgaon (HR)
Email: poonammalik3112@gmail.com
Bioremediation provides a good clean-up strategy whereby the organic wastes are
biologically degraded under controlled conditions, in which micro-organisms are used to
degrade the environment contaminants to a less toxic form. Advancement in science and
technology has increased waste and toxic material, therefore existing remediation
technology is not effective and efficient in removal of toxins from environment. Therefore
preference of nanotechnology in bioremediation is given as nanoparticles exhibit unique
physical and chemical properties, to reach the contaminant zones with less toxic effect on
micro-organisms as well as nanoparticles require less activation energy. Nanomaterials not
only directly catalyze the degradation of wastes and toxins, nanoparticles enhance the
activity of micro-organisms as well as reduction in-overall time consumption and cost.
Nano particles can be used for remediation, sensing, detection and pollution prevention.
Due to their small size and different surface coating, nano particles can enter minute
spaces, can be suspended in water for longer time enabling them to travel farther and
hence better distribution. Despite its various advantages, the main drawback of using nano
particles is that they undergo oxidation while reducing the contaminants. Nanotechnology
will have a positive effect on environmental technology as it offers an eco-friendly
solution to toxic materials that are currently being used.
SP-056
NOVEL CHITOSAN NANO-PARTICLE BASED

COATING FOR FRUIT PRESERVATION
Reena Devi and Veena Jain
Department of Biochemistry, CCS HAU, Hisar
Email: reena.8jan@gmail.com
Tropics and subtropics are the stellar producers of a variety of fruits; majority are
perishable and prone to postharvest decay. Chitosan treatment could keep the freshness of
fruit and reduce fungal decay and microbial growth as well as water loss of samples.
Moreover, coated fruits showed maintenance of their firmness, and all the sensory qualities
during the storage period. Chitosan-based coating was concerned in recent years owing to
its non-toxic, biodegradable, and biocompatible properties. Nanotechnology, most
promising scientific tendency of research that can be employed to enhance chitosan
functionalities for extending fruits shelf life. After applying improved chitosan-based
coating, the preserving effects were increased in most of the cases compared with single
chitosan coating. It has been demonstrated to possess film forming property and
antimicrobial activities against a large spectrum of microorganisms such as bacteria, yeast,
and fungi most likely due to poly-cationic nature. Moreover, it was shown that a semipermeable film created by chitosan acts as a selective barrier to gases diffusion resulting in
procrastinating fruits decay. For instance, it has been reported that strawberries shelf life
could be prolonged just to 7 days employing 1.5% chitosan.
SP-057
NANOTECHNOLOGY IN HORTICULTURAL CROPS

POSTHARVEST MANAGEMENT
Mekala Srikanth, Mamatha N.C., and Telugu Ravi Kumar
Department of Vegetable Science, CCS HAU, Hisar (Haryana)
Email: sricoolhortico@gmail.com
Horticulture science has been defined as science and art of cultivating and handling of
fruits, vegetables, flowers, and ornamental plants. Human population is increasing rapidly,
so providing sufficient and healthy food is becoming a very important problem in the near
future. Now, increasing production efficiency and decreasing post-harvest wastage with
using the findings of novel scientific researches such as biotechnology and nanotechnology
in products, could be counted as the best solution to this problem. Nowadays, a lot of
usages of nanotechnology in horticultural science have been established.The development
of nanotechnology creates an excellent opportunity to address complex technical issues of
food supply chain. Failure to embrace nanotechnology will deny the horticultural sector an
opportunity to capitalize on improved food safety, quality, and securityassociated
economic benefits. Nanotechnology using particular characteristics of nano-particles can
be a very useful technology in all science and industry branches. In relation with extension
of horticultural products shelf life, nanotechnology can help us in some grounds, e.g.
controlling growth and development of microorganisms, introducing a new generation of
packaging coverages (Films) and controlling influence of gases and the harmful rays
(UV), increasing strength, quality and packaging beauty, and using the multiple chips
(Nano biosensors) for labelling products that considered as fundamental step to automated
control of storages.
SP-058
USE OF NANOTECHNOLOGY IN POST HARVEST

MANAGMENTS OF HORTICULTURAL CROPS
Makhan Lal*, D.S. Duhan, V.P.S. Panghal, Shiwani, Anil Duhan2 and Santosh Rani3
Department of Vegetable Science, 2 Department of Agronomy 3 Department of EECM
CCS Haryana Agricultural University-Hisar-125004 (Haryana)
Email: makhanmajoka@gmail.com
India is now the second largest producer of fruits and vegetables in the world. However,
still India is not in a position to provide recommended quantity (120 g/capita/ day) of fruits
and (300 g/capita/day) vegetables for balanced diet to its citizens. Increase in the
productivity is one of the possible ways to meet out the need of the population. However,
increasing the productivity has limited scope because of higher input cost. Therefore,
second feasible option is the hidden harvesting. This includes, post harvest losses of fruits,
flowers & vegetables, which vary from 15-35 % of total production depending upon the
commodities and season. It is very ironic situation that on one side 300 million people are
malnourished while on the other hand, 15-35 per cent fruits & vegetables are being spoiled
because of poor post harvest management of horticultural commodities. So even if we
manage to reduce this amount for 5-10%, huge saves will be obtained. Now, decreasing
post harvest losses with using the findings of novel scientific researches such as
biotechnology and nanotechnology in horticultural produces could be counted as the best
solution to this problem. Now a days, a lot of usages of nanotechnology in agricultural
sciences have been established. In relation with extension of horticultural produces shelf
life, nanotechnology can help us in some grounds, e.g. controlling growth and
development of micro organisms, introducing a new generation of packaging coverages
(Films) and controlling influence of gases and the harmful rays (UV), increasing strength,
quality and packaging beauty, and using the multiple chips (Nanobiosensors) for labeling
products that considered as fundamental step to automated control of storages. First in the
fruit packaging and later in other areas such as tracking, tracing, storage and distribution, is
occurring. Moreover, nano-Ag has the function of absorbing and decomposing ethylene.
The current sensor using electrocatalysis and nanotechnology is a new and promising
technology for affordable detection of ethylene (for apple, avocado, pear and kiwi), which
will enable research in areas where ethylene could not be measured before due to lack of
portable, sensitive, and near real-time measurement. Nanotechnology has shown
significant promise in the enhancement of sensors able to detect spoilage or changes to
product quality.
SP-059
NANOTECHNOLOGY IN POST HARVEST

HORTICULTURE MANAGEMENT: A REVIEW
Ajinath Dukare, Bhushan Bibwe, Bharat Bhushan, and Dattatreya M. Kadam
Horticultural Crop Processing Division
ICAR-CIPHET, Abohar, Punjab, India-152116
Email: ajinath111@gmail.com
Nanotechnology is a new emerging approach that refers to understanding, controlling and

mastering the properties of matter at the nanometer scale. Now a day, nanoparticle science
is becoming one of the most imperative tools in modern agro-horticulture pre harvest and
post-harvest production sectors. Various biological agents such as microbes (Bacillus spp.
Pseudomonas spp. Fusarium spp. Chlorella spp. etc.) and plants/plant extracts (Neem,
garlic, aloe, tea etc.) have emerged as economical and efficient candidates for the synthesis
of nanoparticles by green synthesis approaches. Due to higher solubility, stability and
ecofriendly biodegradable nature of nanoparticles, it finds immense application in
sustainable agro-horticulture food chain. In coming year, findings of novel scientific
approach such as biotechnology and nanotechnology could lead to the increase in
production efficiency, minimizing post harvest decaying wastage with improving
longevity and resistance of horticulture crops products. In relation with post harvest
management and shelf life extension of horticultural crops and its products, nanoparticle
science can help us in some important areas, e.g. In food safety and quality, for controlling
post-harvest crop fungal pathogens and other pathogenic micro organisms, nanopolymer
based antimicrobial packaging for storage of fresh horticulture produce, introducing a new
generation of packaging films and controlling influence of gases and the harmful rays
(UV), developing nanobiosensors for detection food borne pathogens and other
contaminants, developing new plastic for food packaging industry and enhancing strength
and quality of packaging material. Beside this, nanotechnology promises to accelerate the
development of agro-horticulture waste/processed biomass to biofuels production
technologies.
SP-060
ROLE OF NANOTECHNOLOGY ON SOIL

AND CROPS
Md. Sarware Alam, K. S. Grewal Pradyuman Singh, Abhinaw kumar Singhand Jitendra Kumar
Department of Soil Science, College of Agriculture, CCS Haryana Agriculture University, Hisar
Email: junedkhan.24@gmail.com
The current challenges of sustainability, food security and climate change are engaging
researchers in exploring the field of nanotechnology as new source of key improvements
for the agricultural sector. Agricultural growth in India is showing negative trend from
3.6% during 1985-95to less than 2% in the decade 1995-05 against a targeted average
annual growth of 4%for the agricultural sector to 2020. , nanotech based control of
agriculture related diseases also can eliminate by accumulation of several pesticides from
agricultural field from different sources of host and also use of several chemical fertilizers
which is one of the hot issues for todays agriculture,The technology uses in Crop
production Fertilizers macronutrient Fertilizers Coated with Zinc Oxide Nanoparticles Soil
improvement Water/liquid retention Nanomaterials, e.g. zeolites and nano-clays, for water
or liquid agrochemicals retention in the soil for their slow release to the plants Plant
breeding, Nanomaterials from plant,
An important reason for the low macro and micro nutrient use efficiency (MUE) is the lack
of synchronization between the fertilizer-MNs release and their crop demand during the
growing season. It is very difficult for current conventional technologies to enable
synchronization of fertilizer-nutrient release according to crop demand. Modern research
efforts are conducted to increase MUE, paying special emphasis on potential contributions
of nanotechnology and nanobiotechnology; however, their application in fertilizer nutrient
delivery is at a nascent stage. In Canada, a multidisciplinary team of scientists are
attempting to synchronize the release of urea-N according to crop demand. This approach
uses knowledge from soil-plant ecology, together with tools, devices and materials from
nanotechnology and nanobiotechnology to develop an intelligent nanofertilizer platform
for efficient nutrient delivery. If these issues are taken care of, nanotechnologic
intervention in farming has bright prospects for improving the efficiency of nutrient use
through nanoformulations of fertilizers, breaking yield barriers through
bionanotechnology, surveillance and control of pests and diseases, understanding
mechanisms of host-parasite interactions at the molecular level, development of newgeneration pesticides and their carriers, preservation and packaging of food and food
additives, strengthening of natural fibers, removal of contaminants from soil and water,
improving the shelf-life of vegetables and flowers, clay-based nanoresources for precision
water management, reclamation of salt-affected soils, and stabilization of erosion-prone
surfaces.
SP-061
NANOSENSORS: THE FUTURE OF FOOD SAFETY

AND QUALITY DETECTION
Pratibha Yadav, Rizwana Rehsawla and Nayan Tara
Department of Molecular Biology, Biotechnology and Bioinformatics, CCSHAU, Hisar, Haryana

Email: Pratibhayadav377@gmail.com
The identification and detection of food borne pathogens continue to lean on conventional
culturing techniques. These techniques are time consuming, elaborate and are not suitable
for on-site monitoring as they need to be completed in a microbiology laboratory. Many
research focused on development of reliable, rapid, sensitive and specific method for
detection of target analyte. Biosensor technology has the potential to increase sensitivity
and specificity, speed up the detection, enable high-throughput analysis, and to be used for
critical control points monitoring in food production. The water, food and safety
improvement security is based on the ability to identify, detect and trace water and food
pathogen. Biosensors are thrilling alternative to the traditional methods for the detection of
toxins and pathogens in food and there sensitivity depends on transducers properties and
on the recognition element. A nanoparticle based bioassay is developed by research group
which can rapidly detect E.Coli O157:H7 in food. This is highly infectious strain that
causes dangerous food-borne disease. These technology uses silica nanoparticle doped
with fluorescent dye molecule and antibodies which themselves attaches to bacterium and
react with antigen on the bacteria surface. The dye produces fluorescent signal when
antigens and antibodies react and it is effectively amplified, allowing the bacterial
concentration to be determined readily using fluorescence microscopy and spectrofluorometric analysis. This technique can also be applied to check presence of multiple
contaminants simultaneously by adding different antibodies to the nanoparticles.
Generally, sensors /nanosensors are placed in food packaging to control internal and
external conditions of food.
SP-062
EFFECT OF NANOPARTICLES ON PLANTS WITH

REGARD TO PHYSIOLOGICAL ATTRIBUTES
G. Chandramohan Reddy1, G. Rajeshwari2
Dept. of Plantation, Spices, Medicinal and Aromatic crops, College of Horticulture,

Anantharajupeta,
Dr. YSRHU, A.P
2
Dept. of Entomology, College of Horticulture, Bengalore,
University of Horticultural Sciences,
Karnataka
Email: reddyhorti96@gmail.com
Nanotechnology has emerged as an indispensable tool for the future, with its reach
spanning across diverse domains. Such a rapid advance has resulted in the exodus of
various types of nanomaterials into the environment. Thus, it becomes essential to
understand the imminent effects, either advantageous or deleterious, of these
nanomaterials on the living subjects advertently or inadvertently exposed to them.
Numerous studies have focused on the effects of such nanomaterials in the nanoparticulate
form on the mammalian system, with increased studies on the plant system as well. Due to
the complex nature of uptake and translocation mechanism present in plants, it has been
relatively difficult to unanimously devise a general dataset of the effects that nanoparticles
(NPs) have on them. Research over the past years has documented mostly toxic effects of
the NPs, either during the germination stage or with respect to the shootroot length, while
few others have explored the possibilities of utilizing them as carriers for chemicals as
herbicides, pesticides, fertilizers, or in some cases genes. There have been numerous
contradictory findings with some reports suggesting growth enhancing effects and others
observing retarding effects of similar NPs on similar or different plant species. Such
contradictions and lack of conclusive observations has slowed down the impact of
nanotechnology in the agriculture industry when compared with the medical scene. This
scenario demands a comprehensive calibration of the analysis and interpretation of NP
plant interaction and effects thereof from the physiological, biochemical, and
photosynthetic level to the molecular level to decisively devise a verdict on the actual
effects of nanoparticles on the plant system.
SP-063
CARBON NANOTUBE AND ITS BIO-SENSING

APPLICATIONS IN AGRICULTURE
KP Singh
e-Governance Division, Indian Council of Agricultural Research, Krishi Bhawan, New Delhi-110001
Email: kpsingh@icar.gov.in
The integration of 1D nano-material such as carbon nanotubes into electrical devices offers
substantial advantages for the detection of chemical and bio-molecules and has significant
advantages over conventional detection methods. Every atom of single wall carbon
nanotube is on the surface and exposed to the surrounding, so even a small change in
charge environment may lead to the drastic change in electrical properties of devices made
of CNTs. The diameter of single wall carbon nanotube is also comparable to the size of
single molecules like DNA, Virus. The size compatibility and biocompatibility also makes
CNTs a perfect choice for bio-sensing applications. Semiconducting single wall Carbon
Nanotubes (CNTs) are used to fabricate Field Effect Transistor (FET) devices which can
be used for detection of molecules.
CNT based FET in which a network of CNTs was attached with source and drain
electrodes and CNTs were aligned by applying electric field across the electrodes. A
variety of proteins can strongly bind to the nanotube surface. That binding or
functionalization is monitored by the change in conductance in CNT based FET. The
change in conductance occurs due to the transfer of the charge between CNTs and the
protein. Similarly, CNT based biosensor can be developed for the detection of pathogen in
food, toxic chemicals and pesticide residue in grains and infectious disease in crops. Some
study suggests that even a single virus can be detected by CNT based sensor.
In agriculture, biosensors have great promises such as pathogen detection in food
processing industry, pesticide residue in crops, veterinary diagnosis and infectious disease
identification in crops.
SP-065
NANOTECHNOLOGY: A NEW FRONTIER IN

AGRICULTURE
Asha Rani, Sumit Jangra, Rekha Patel & R. C. Yadav
Department of Molecular Biology, Biotechnology & Bioinformatics
CCS Haryana Agricultural University, Hisar
Email: sainias007@gmail.com
Nanotechnology is one of the most important tools in modern agriculture. Agri-food

nanotechnology is anticipated to become a driving economic force in the near future. It has
many applications in all stages of production, packaging, processing, storing, and transport
of agricultural products. The reduced use of herbicides, pesticides and fertilizers with
increased efficiency, controlled release and targeted delivery will lead to precision
farming. The interest is increasing with suitable techniques and sensors for precision in
agriculture, natural resource management, early detection of pathogens and contaminants
in food products and smart delivery systems for agrochemicals like fertilizers and
pesticides. The problems of agriculture like over-dependence on supplementary irrigation,
vulnerability to climate, and poor input and energy conversion to products can be solved
by using nanotechnology, provided agricultural scientists seek a chance to try and
cooperate with scientists of kindred disciplines. Success has been achieved for
manufacturing nano-pesticides and nano-fertilizer in food packaging, use of agricultural
waste, nanosensors, precision agricultural practices, and in livestock and fisheries. Todays
nanotech food products include a new variety of canola oil containing tiny materials that
can block cholesterol from entering the bloodstream. Nanotechnology devices and tools,
like nanoparticles, nanocapsules and even viral capsids, are examples of uses for the
detection and treatment of diseases, enhancement of nutrients absorption by plants,
delivery of active ingredients to specific sites and water treatment processes. Use of targetspecific nanoparticles can reduce the damage to non-target plant tissues and the amount of
chemicals released into the environment. Nanotechnology derived devices are also
explored in the field of plant breeding and genetic transformation.
SP-065
NANOTECHNOLOGY: AN INTERESTING AND

CRUCIAL TOOL IN MODERN AGRICULTURE
Bharat Taindu Jain, Raman Sharma, Hirdayesh Anuragi
Department of Genetics & Plant Breeding, CCS Haryana Agricultural University, Hisar- 125004, India
Email: jainbharat91@gmail.com
Nanotechnology is the design, characterization, production and application of structures,

devices and systems by controlling shape and size at nanometer scale.In agriculture,
technical innovation is of importance with regard to addressing global challenges such as
population growth, climate change and the limited availability of important plant nutrients
such as phosphorus and potassium. The application of nanomaterials in agriculture aims in
particular to reduce applications of plant protection products, minimize nutrient losses in
fertilization, and increase yields through optimized nutrient management. Nanotechnology
devices and tools, like nanocapsules, nanoparticles and even viral capsids, are examples of
uses for the detection and treatment of diseases, the enhancement of nutrients absorption
by plants, the delivery of active ingredients to specific sites and water treatment processes.
The use of target-specific nanoparticles can reduce the damage to non-target plant tissues
and the amount of chemicals released into the environment. Nanotechnology derived
devices are also explored in the field of plant breeding and genetic transformation. In
recent years, agricultural waste products have attracted attention as source of renewable
raw materials to be processed in substitution of fossil resources for several different
applications as well as a raw material for nanomaterial production. The results open a wide
range of possibilities for using Nanoparticles in general plant research and agronomy.
Nanotechnology improves their performance and acceptability by increasing effectiveness,
safety as well as ultimately reducing health care costs.

SP-066
NANOTECHNOOGY-A NEW FRONTIER TO

AGRICULTURE FOR ENHANCING CROP
PRODUCTIVITY: A REVIEW
B. S. Duhan and Hardeep Singh Sheoran
Department of Soil Science, CCS Haryana Agricultural University, Hisar-125004
Email: dr.bsduhan@gmail.com
Nanotechnology is a technology which deals to conduct the research work at a nanoscale

which is about 1 to 100 nanometers, where unique physical properties make its
applications possible in every sphere. As world population grows the demand for more and
more food grains increases and ultimately the pressure on land and shrinking natural
resources increased drastically. With this increased demand for growing more and more
food grains the researches have realized that conventional farming technologies would
neither be able to increase productivity nor to improve the input use efficiency. The
research work in nanotechnology started for industrial application nearly half the century
ago and nanotechnology in agriculture has gained the momentum in the last decade with
an abundance of public funding, but its pace of growth is still modest, even though many
disciplines are covered under the umbrella of agriculture. Nanotechnologic intervention in
agriculture has bright prospects for improving the nutrient use efficiency of very critical
nutrient element like phosphorus through nano-formulations of fertilizers, extending
achievable yield targets through bio-nanotechnologies, control of insect-pest infestations
by understanding the host-pest interactions at molecular level, reclamation of salt affected
soils and many more applications in agriculture make it suitable for its prior use in crop
production which plays a crucial role in plant growth and ultimately in crop productivity.
Moreover, we should set the agenda for more and more nanotechnology research in
agricultural applications to achieve the target of sustainability in terms of enhancing the
crop productivity through judicious use of available resources. Thus nanotechnology being
a new entrant in agriculture, we need to revisit the contemporary theoretical foundations
and practices of agriculture to conform to next generation farming.
SP-067
POTENTIAL APPLICATIONS OF
NANOTECHNOLOGY IN HORTICULTURE
Payal Rana1, Rahul Pippal2 and Swetal Rana2
Krishi College, Jorapali, Raigarh (496001) C.G., 2College of Agriculture, RVSKVV, Gwalior (474020)
(M.P.)
Email: payalnewmoon@gmail.com
Horticultural products waste is estimated around 20- 30% in developing countries, so even
if we manage to reduce this amount for 5-10%, huge saves will be obtained. Reducing
these losses can not only improve farmers incomes but could also encourage more
consumption of this highly nutritious fruit in a region where per capita consumption is
only half of the recommended level. Now, increasing production efficiency and decreasing
post-harvest was tage with using novel sciences such as biotechnology and
nanotechnology in products, could be counted as the best solution to this problem.
Nanotechnology is a new approach that refers to understanding and mastering the
properties of matter at the nano-scale: one nano-meter (one billionth of meter) is the length
of a small molecule. At this level, matter exhibits different and often amazing properties
and the borders between established scientific and technical disciplines fade.
Nanotechnology has already been applied to the management of supply chain processes
associated with food quality, handling, packaging, and safety. In the field of agricultural
supply chains, nanotechnology deployment is already bringing potential benefits to
farmers, the food industry and consumers alike, through innovations in agri-food
production, processing, preservation and packaging.
SP-068
GREEN SYNTHESIS OF SILVER NANOPARTICLES:

A RECENT ECOFRIENDLY APPROACH
Mamta Sharma and Minakshi Sharma
Department of Zoology, Maharshi Dayanand University, Rohtak-124001, Haryana, India.
Email: sharmamta760@gmail.com
Nanotechnology is concerned with the synthesis of nanoparticles of various size and shape.
Green synthesis of silver nanoparticles is found to be an emerging trend of
nanotechnology. For the synthesis of nanoparticles .plant leaf extract offers numerous
ecofriendly benefits and they have biocompatibility for various pharma and biomedical
applications. Silver nanoparticles has been considered as novel therapeutic agent having
anti bacterial ,antifungal,anti viral and anti inflammatory activities. The green synthesis of
AgNPs can be efficiently applied for future engineering and medical concerns.
Conventionally silver nanoparticles are synthesized by various chemical method using
chemicals as reducing agents but these method have been known for their general toxicity;
therefore it is needed to develop environment friendly approach. Biological approaches are
coming up to fill the empty space for instance green syntheses using biological molecules
derived from plant sources in the form of extracts exhibiting superiority over chemical
methods.
SP-069
SOIL MICROBIAL DIVERSITY IN ORGANIC AND

CONVENTIONAL FARMING SYSTEMS IN HARYANA
Hardeep Singh Sheoran1, V.K. Phogat1, Rita Dahiya1 and Rajesh Gera2
1
Department of Soil Science, 2Department of Microbiology,

CCS Haryana Agricultural University, Hisar-125004 (Haryana, India)
Email: sheoranhardeep2008@gmail.com
Organic agricultural systems aim at reducing the use of synthetic fertilizers and pesticides
in order to improve sustainable production and ecosystem health. Despite the integral role
of the soil microbes in agricultural production, there is a limited understanding of the
complex response of microbial diversity to organic and conventional farming systems. The
objective of the study was to evaluate the impact of organic and conventional management
practices for fields, particularly under vegetables and horticultural crops on soil microbial
diversity. Soil samples from 25 fields under organic farming as well as adjoining
conventional farming were collected from 0-15 and 15-30 cm depths and assayed for
viable counts of bacteria, actinomycetes and fungi. Soil microbial populations as well as
diversity were higher in soils under organic farming, indicating higher microbial activity
under organic farming. Populations of fungi were significantly higher in soils from organic
than conventional fields. The diversity of bacterial communities was also greater in soils
under organic farming. Among the 25 sampling sites, at one of the sites efficient strain of
phosphorus solubilizing fungus belonging to Aspergillus sp. was identified and isolated for
further studies and use. The study concluded that soils under organic farming had higher
microbial diversity which is essential for enhancing soil production and other functions of
soil in the given ecosystem.
SP-070
NANOTECHNOLOGY APPLICATIONS IN
AGRICULTURE
Nayan Tara1, Rizwana Rehsawla1, Pratibha Yadav1, Shikha Yashveer1, A. Chaudhury2

1
Department of Molecular Biology, Biotechnology and bioinformatics, College of Basic Sciences and
Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana
2
Centre for Bio and Nano-Technology, Department of Bio and Nano-Technology, GJU S&T, Hisar
Email: nayansheoran29@gmail.com
Agriculture is considered as the backbone of most developing countries including India

and means of livelihood for more than 60% population in India. Agriculture provides food
for human consumption, feed for animals and biofuel production. Global climate change is
posing threats to agriculture in form of salinity, drought, nutrient availability, biotic stress.
In view of increasing population pressure, it is essential to apply the modern technologies
such as nanotechnology and nanobiotechnology in agricultural sciences to ensure food
security. Nanotechnology has a great potential to increase agriculture productivity and has
been tapped in various ways to make agriculture more productive and as well as more
sustainable. Pesticide filled nanoparticles and nanotubes can be used to control different
pests in an environment friendly way. Nanotechnology has been used for management of
agriculture waste and help in reducing environmental pollution. For herbicide control,
nanoparticles which attack seed coat of weed and stop its germination have been used.
Nanofertlizers increases nutrient efficiency whuch inturn enhance the crop productivity by
mechanism of slow and targeted release. In brief nanotechnology has a lot of potential to
increase the agriculture productivity which is yet to be tapped. But as this technology is in
infant stage, so bioethical and biosafety issues related with use of nanoparticles in
agriculture must be carefully evaluated maintaining the balance between its application
and bisafety evaluation.
SP-071
AGRONANOTECHNOLOGY: FUTURE OF
AGRICULTURE
Zeenat Wadhwa1, Rajrani1, Kanchan Kamra1, Sumit Jangra2
1
Dayanand College, Hisar

Email: zeenatwadhwa.12@gmail.com
Nanoscience emerged in the late 1980s and is developed and applied in India since the
middle of the 1990s. In fact, the country is in need of a Second Green Revolution.
Nanoscale science and nanotechnologies are envisioned to have the potential to
revolutionize agriculture and food systems. The successful application of various
nanoplatforms in medicine under in vitro conditions has generated some interest in agrinanotechnology. This technology holds the promise of controlled release of agrochemicals
and site targeted delivery of various macromolecules needed for improved plant disease
resistance, efficient nutrient utilization and enhanced plant growth. The reduced use of
herbicides, pesticides and fertilizers with increased efficiency, controlled release and
targeted delivery will lead to precision farming. Dream of automated, centrally controlled
agriculture can become reality now. Modern agriculture is need of hour because
conventional agricultural will not be able to feed an ever increasing population with
changing climate, depleting resources and shrinking landscape. New tools are underway
which will be equipped with nanodevices capable of replacing many cellular types of
machinery efficiently. Use of nanotechnology could permit rapid advances in agricultural
research, such as reproductive science and technology which will produce large amount of
seeds and fruits unaffected by season and period, early detection of stresses and alleviating
stress effects and disease prevention and treatment in plants. Specifically, application of
nanoparticle technology in plant pathology targets specific agricultural problems in plant
pathogen interactions and provide new ways for crop protection. Smart sensors and smart
delivery systems will help the agricultural industry combat viruses, spores and other crop
pathogens. Occupational exposure with nanotechnologies will increase with their growing
production and use in society.
SP-072
GENETICALLY ENCODED FRET-BASED

NANOSENSOR: AS A TOOLBOX FOR PLANT SCIENCE
Rizwana Rehsawla, Pratibha Yadav, Reema Rani, Nayan Tara
Department of Molecular Biology, Biotechnology and Bioinformatics, CCSHAU, Hisar, Haryana

Email: rizwana.rehsawla1992@gmail.com
The development of Plant is highly interconnected with the metabolic state of cells and
tissues. Metabolic networks determine the growth and development of plants, reproduction
and senescence. Tangled networks of metabolic reactions are regulated by gene regulatory
networks, nutrient availability and environmental conditions to measure signaling
molecules, such as hormones, sugars and calcium. Also used for monitoring the metabolic
state via energy equivalents and pH with the help of different biosensors. Current
knowledge of metabolic reactions is based on genetic manipulations of the enzymes that
drive these reactions and their biochemical analysis in vitro. There is a need of genetically
encoded biosensors to detect metabolites directly to concede the kinetics of metabolite flux
and the links of metabolic state and development in vivo. Genetically encoded sensors
such as fluorescent probes used for small molecules have capaciously extended the toolbox
for plant sciences. The application of genetically encoded fluorescent sensors and reporters
is that they directly provide information on changing levels of small molecules with high
spatiotemporal resolution. This promising approach represents the development of single
fluorophore sensors as they allow the combination of different sensors with little spectral
overlap. FRET-based imaging provides an easy method to monitor analyte changes in
living cells and their compartments. The generation of stable plant lines expressing
optimized sensors may provide suitable tools for a variety of applications. 100 different
genetically encoded biosensors have been developed for targets as diverse as ions,
molecules and enzymes providing a window into the real-time biochemistry of living cells.
SP-073
RELEVANCE OF NANOTECHNOLOGY IN THE

FIELD OF AGRICULTURE
Jaibir Tomar* Vinay Kumar ** and Rajeev Kumar***

*Department of Agronomy, Janta Vedic College, Baraut, Baghpat, Uttar Pradesh (India)-2506111
**Department of Physics, Janta Vedic College, Baraut, Baghpat, Uttar Pradesh (India)-2506111
***Department of Physics, D. J. College, Baraut, Baghpat, Uttar Pradesh (India)-250611
Email: dr.jaibirtomar@yahoo.in
Nanotechnology is the design, characterization, production and application of structures,

devices and systems by controlling shape and size at nanometer scale. At present
scenario, in the field of agriculture nutrient management and fertilizer uses to enhance
the supplements of soil nutrients, so by the use of fertilizer and nutrient management we
can increase plant growth, crop productivity and food quality. As a result, crop
production and global food security are highly dependent on fertilizers. The selection
and deployment of fertilizer requires the uses of nono-technological tools. Pesticides use
has dramatic consequences in both developed and developing countries. Pesticide
chemicals may induce oxidative stress leading to generation of free radicals and
alterations in antioxidants or Oxygen Free Radical scavenging enzymes. Plantpathogen
interactions and provide new ways for crop protection. Nanotechnology considers the
topic with virus and other pathogens scale. Therefore, it has high potential for identify
and eliminate pathogens.
SP-074
THE NANOWORLD IN AGRICULTURENANOTECHNOLOGY

Kirti Mehta, Zeenat Wadhwa, Nikita Baliyan, Shikha Yashveer and K.S. Boora
Department of Molecular Biology, Biotechnology and Bioinformatics,
CCS HAU, Hisar-125004
Email: kirtimehta88@gmail.com
Nanotechnology is defined by the US Environmental Protection Agency as the science of

understanding and control of matter at dimensions of roughly 1100 nm, where unique
physical properties make novel applications possible. It is now more properly labeled as
"molecular nanotechnology" (MNT) or "nano-scale engineering. These materials
display different properties from bulk materials due to their size. These differences
include physical strength, chemical reactivity, electrical conductance, magnetism and
optical effects. There are two process for nanomaterial creation including "bottom-up"
processes (such as self-assembly) that create nanoscale materials from atoms and
molecules, and "top-down" processes (such as milling) that create nanoscale materials
from their macro-scale counterparts. Nanotechnology has the potential to revolutionize
the agriculture with new tools for the rapid disease detection and their treatments,
enhancing the ability of plants to absorb nutrients, increasing the efficiency of pesticides
and herbicides. The potential of nanotechnology in agriculture are precision farming,
smart delivery systems (delivery of nanocides-pesticides encapsulated in nanomaterials),
food industry, food processing, packaging, food safety, etc. Nanoparticles can be used as
biomarkers or as rapid diagnostic tool for detection of bacterial, viral and fungal plant
pathogen in agriculture. Smart sensors and smart delivery systems will help the
agricultural industry combat viruses and other crop pathogens. Nanomaterials, in
addition to its use for pesticide and herbicide detection, have also been applied for
pesticide degradation. Overdependence on supplementary irrigation, vulnerability to
climate, and poor input and energy conversion are the three dominant issues in the
current agricultural production system, and nanotechnology could possibly reduce their
impact.
SP-075
NANOPARTICLES AS AN EFFECTIVE TOOL IN

CONTROLLING THE PLANT DISEASES
Annu Devi, Dr. Asha Sharma, Pooja, Pawan Sindhu
Department of Botany M.D.University, Rohtak (Haryana) 124001
Email: annusangwan92@gmail.com
Nanotechnology is an interdisciplinary field that has been entered in different range of

applied sciences such as chemists, physicists, biologists, medical doctors and engineers.
In the agricultural sector, nanotech research and development is likely to facilitate and
frame the next stage of development of genetically modified crops, animal production
inputs, chemical pesticides and precision farming techniques. Some of the nano-particles
that have entered into the arena of controlling plant diseases are new forms of carbon,
silver, silica and alumino-silicates. Nanotechnology has astonished scientific community
because at nano-level, material shows different properties. The use of nano size silver
particles as antimicrobial agents has become more common as technology advances
making their production more economical. Since silver used for controlling various plant
pathogens in a relatively safer way as compared to commercially used fungicides. Silver
is known to affect many biochemical processes in the microorganisms including the
changes in routine functions and plasma membrane. Use of nanoparticles has been
considered an alternate and effective approach which is eco-friendly and cost effective
for the control of pathogenic microbes.
SP-076
NANOTECHNOLOGY: THE NEW PERSPECTIVE IN

PRECISION AGRICULTURE
Ravinder Kumar, Joginder Singh Duhan

Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-125055, Haryana, India.
Email: duhanjs68@gmail.com, duhanjs@rediffmail.com
Nanotechnology is an advanced field of interdisciplinary research in fields like medicine,

pharmaceuticals, material science, electronics and agriculture. The green revolution
resulted in blind usage of pesticides and chemical fertilizers which caused loss of soil
biodiversity and nitrogen fixation capacity of useful microbes; developed resistance
against pathogens and pests as well. In recent past efforts have been made by researchers
to improve agricultural yield through exhaustive research in nanotechnology. These
include nanoparticle-mediated gene or DNA transfer for incorporation of desired trait in
plants. Encapsulation of nanoparticles in conventional fertilizers, pesticides and
herbicides helps in slow and sustained release of nutrients water and agrochemicals
resulting in precise dosage of required material to the plants. Advanced biosensors and
remote sensing devices required for precision farming is possible only by nanochips.
Nanotechnology based plant viral disease detection kits are also becoming popular and
are useful in speedy and early detection of viral diseases which are otherwise difficult to
control.In this article, the potential uses and benefits of nanotechnology in precision
agriculture are to be discussed. The modern nanotechnology based tools and techniques
have the potential to address the various problems of conventional agriculture and
revolutionize this sector.
SP-077
EFFECT OF DOPING ON MAGNETIC PROPERTIES

OF FERRITE NANOPARTICLES
Nisha Rani
Department of Materials Science and Nanotechnology, DCRUST, Murthal
Email: nisha.punia19@gmail.com
Superparamagnetic spinel ferrites MFe2O4 (M = Mn, Fe, Co, Ni) are currently
considered among the most successful MNPs for medical applications, including contrast
enhancement in magnetic resonance imaging (MRI) (Jun et al. 2008), magnetically
guided drug delivery (Neuberger et al. 2005), and hyperthermia cancer therapy (Hergt et
al. 2006). This enhancement is very important for the successful and minimum dosage
performance of MNPs in medicine. Finite-size and surface effects in magnetic
nanoparticles (MNPs) give rise to magnetic properties significantly different from the
corresponding bulk materials. The structural and magnetic properties of spinel ferrite
nanoparticles are strongly influenced by their composition and microstructures, which
are sensitive to the preparation methods. The reduction in the particle size of these
ferrites leads to the variation in higher values of coercivity, lower values of saturation
magnetization and reduced or enhanced magnetic moments etc. ferrites are most
appealing for the purpose of controlled drug administration because of their
biocompatibility and superparamagnetic behavior, facilitating the channeling of the drug
towards specific target cells, by externally controlling its path using a magnetic field.
Zinc ferrite nanoparticles were synthesized with composition ( = 0; 0.2, 0.3, 0.4, 0.5)
have superparamagnetic properties.The saturation magnetization at 0.4 is 75emu/g.
(Iryna Vedernikova, 2015). The superparamagnetic Mn-Zn ferrite nanoparticles were
synthesized with doping of manganese with composition (x=0.2, 0.5 and 0.8).The
saturation magnetization of 84.5 emu/g has occurred in case of high manganese content
sample (Mn0.8Zn0.2Fe2O4).
SP-078
INEXPENSIVE, SCALABLE AND RAPID

PROTOTYPING OF ORGAN-ON-CHIP FOR CANCER
METASTASIS USING CO2 LASER ENGRAVER
Assim Verma, Dr Bhanu Prakash, Dr Deepika Sharma
Institute of Nanoscience and Technology, Mohali
Email: assimdlhi1@gmail.com
Increasing recognition of 3D cell culturing as highly imperative technique to better

understand the actual cancer physiology has led to the development of various organonchips. Despite of their promising results and clinical importance its wide scale
adoption is limited due to its dependency on photolithography process which is laborious
and expensive, also requires sophisticated facility and trained technicians. To bridge this
gap we have fabricated PDMS based organ-on-chip with prostate cancer metastasis
modelling using inexpensice CO2 laser engraver with design-to-device time less than 2
minutes and cost of fabricated chip less than 20.
SP-079
APPLICATION OF THERAPEUTIC BENEFITS OF

SINIGRIN BY USING GREEN NANOBIOTECHNOLOGY
Manju Yadav
Department of Genetics and Plant Breeding, College of Agriculture, CCS HAU, Hisar
Email: amanju.2k8@gmail.com
The biogenic synthesis of nanoparticles opens a new possibility of conveniently

synthesizing metallic nanoparticles using natural products. Recently silver and gold
antimicrobial nanoparticles have been synthesized using the plant extracts of
Brassicaceae. The secondary metabolites in plants have been used in the formulation of
nanoparticles through increase the effectiveness of therapeutic compounds, while
minimizing side effects for being: rich source of bioactive chemicals, biodegradable in
nature and non-polluting (eco-friendly). Glucosinolates are a class of abundant
secondary metabolites characteristic of the plants of the mustard family (Brassicaceae).
Sinigrin (allyl-glucosinolate or 2-propenyl) is a glucosinolate naturally present in
mustard seeds and other edible parts from the Cruciferae family. A number of studies
have been performed on the therapeutic activities of sinigrin and revealed its anticancer,
anti-inflammatory, antibacterial, antifungal, antioxidant, and wound healing effects. The
hydrolysis of sinigrin leads to the formation of several compounds, including allyl
isothiocyanate (AITC), allyl thiocyanate, allyl cynide, and 1- cyano- 2,3- epithiopropane
which are responsible for all therapeutic activities. Defatted mustard seed meal powder
(MSMP) is a natural source of AITC.
So this approach of synthesis of nanoparticles from mustard seed will be hopeful step in
application of nanoparticles for therapeutic benefits. Furthermore, promising
investigations in the area of drug carrier systems can be a useful approach to enhance the
therapeutic uses of sinigrin.
SP-080
RADISH (RAPHANUS SATIVUS L.): A SOURCE

OF BIOACTIVE PHYTOCHEMICALS FOR
NANOENCAPSULATION
Rajita Beniwal, Sushila Singh and V.K. Madan
Department of Chemistry and Biochemistry, College of Basic Sciences and Humanities, Chaudhary
Charan Singh Haryana Agricultural University, Hisar, Haryana
Email: rajitabeniwal14496@gmail.com
Bioactive phytochemicals are one of the most important sources of food and vegetables.
Compared to other vegetables Brassica vegetables have higher bioactive phytochemicals
which make them very interesting crop from the consumers point of view. These
vegetables contain bioactive substances with a potential for reducing the physiological as
well as oxidative stress and are good source of phytochemicals for our health promoting.
Raphanus sativus is an important vegetable related to Brassicaceae family. A high intake
of Radish reduces the risk of age related chronic illness such as cardiovascular health and
other severe health related diseases. Keeping in view the above concerns, the present
study was done to evaluate the bioactive compounds of Radish. Roots of Radish
(Raphanus sativus) were shade dried followed by oven drying and moisture content was
estimated. Various chemicals and phytochemicals like minerals, crude protein, sugar,
crude fibers, flavonoid and phenols were present. After drying, roots were grinded and
aqueous and ethanolic extracts of Radish roots powder were prepared. From these
aqueous and ethanolic extracts of Radish roots powder, extracted phytochemicals will be
used for nanoencapsulation for better bioavailability.
SP-081
CASEIN MILK PROTEIN FIBER- A NEW

INNOVATIVE FIBER
Gupta, R. and Arya, N.
Department of Textile and Apparel Designing,
I.C College of Home Science, CCS HAU, Hisar- 125004
Email: renurenu902@gmail.com
Milk protein fibre is made out of skimmed milk. Main components of this fibre are casein
proteins, drawn from the cows milk. It is responsible for the white, opaque appearance of
milk in which it is combined with calcium and phosphorus as clusters of casein molecules,
called micelles. This fiber contains eighteen type of amino-acids extracts that helps in the
nourishments of the skin and makes it healthier. Milk protein fibre is a blend of nature,
science and technology that has benefits of natural as well as synthetic fibre. Milk fiber
was invented in 1930s in both Italy and America and was called milk casein. A new
generation of innovative fiber and a kind of synthetic fiber made of milk casein through
bioengineering method with biological health care function and natural & long-lasting
antibacterial effect, which has got valid certification for international ecological textile
certification. It is hygienic, flexible, smooth, sheen, renewable, biodegradable and eco
friendly fabric but it has low durability and is expensive. The mass specific resistance of
milk protein fiber is large. Milk protein fiber is bulky and it is easy to open. The cohesion
force is relatively weaker. Casein fibres resemble wool in having a soft warm handle.
Casein fibres provide good thermal insulation. They are resilient like wool. The process of
making milk protein fiber include: - Desizing, scouring, bleaching, dyeing, drying,
printing, finishing, carbonising. Milk fibers can be blended with many other fibers like
cotton, cashmere, silk etc. The healthy nature of milk fiber is considered as a perfect
material for manufacturing of underwear's. Milk casein protein are considered as a main
ingredient of milk protein fiber, which can lubricate the skin. The milk proteins contain the
natural humectants factor which can help to maintain the skin moisture, to reduce the
wrinkles & to smoothen the skin. Milk fiber can be used for making of garments,
beddings, socks, sportswears, new borns bath towels etc. The milk protein fiber is a
healthy and comfortable fiber that makes us feel better and will certainly become popular
in the market.
SP-082
NANOINFORMATICS: AN APPROACH IN MEDICINE

AND DRUG DESIGNING
Palak Singh and Sunita Jain
Department of Molecular Biology Biotechnology and Bioinformatics, College of Basic Sciences, CCS
Haryana Agriculture University, Hisar, Haryana
Email: palak03singh@gmail.com
Bioinformatics and Nanotechnology are intrinsically engineering disciplines. Application

of Bioinformatics in the field of nanotechnology has opened up new gates for the
development of nanomaterials. Nanoinformatics has recently emerged to address the need
of computing applications at the nano level. Nanoinformatics refers to the use of
informatics techniques for analyzing and processing information about the structure and
physico-chemical characteristics of nanoparticles and nanomaterials, their interaction with
their environments, and their applications for nanomedicine. Nanotechnology and
Bioinformatics are quickly evolving into a research field that encompasses the use of all
kinds of biomedical information, from genetic and proteomic data to image data associated
with a particular disease condition of a patient. Development of new tools and
technologies that are critical for the designing, modeling, simulation and visualization of
nanosystems that have arisen during the accelerated evolution of Nanobioinformatics
applied to medical science. Recent advances have made in biomarker discovery,
biocomputing and nanotechnology that has raised new opportunities in the rising fields of
personalized medicine and predictive. Recent developments in nanotechnology are
enabling biologists to address fundamental questions about how proteins function.
Bioinformatics role in the field of nanotechnology can facilitate the management and
integration of heterogeneous information, defining nomenclatures, taxonomies and
classifications for various types of nanomaterials, and research on new modeling and
simulation techniques for nanoparticles. Informatics tools can be invaluable in accelerating
the design and implementation of nanoparticles and devices and the evaluation of in vitro
and in vivo applications, which will help advance nanomedicine.
SP-083
NANOPARTICLES FOR SiRNA DELIVERY

Preeti, Neha and Sumit Jangra
Department of Molecular biology, Biotechnology and Bioinformatics
Email: preetijangra779@gmail.com
Short interfering RNA (siRNA) can be used as laboratory tools for modulating gene
expression and for treatment of various diseases like cancer, genetic diseases and
resistant viral infections. siRNA can also be used to better understand the function of
non-protein coding genes through RNA interference, a post-transcriptional gene
regulatory mechanism. However, delivery of siRNA into the target cells is hampered by
the difficulty of safely and effectively introduction. To overcome this, nanoparticles
mediated delivery has recently emerged as a very powerful and efficient technology.
Nanoparticles have been developed and used for systemic delivery of siRNA which
include silica and silica based nanoparticles, metal and metal oxides nanoparticles,
carbon nanotubes, grapheme, dendrimers, polymers, cyclodextrins, lipids, hydrogels and
semiconductor nanocrystals. These different nanoparticle systems offer various
advantages and disadvantages based on their composition, physical, and chemical
characteristics, thus leading to a range of effectiveness when associated with siRNA. The
development of multifunctional and novel nanoparticles concerning siRNA delivery to
reach the full potential of siRNA therapeutics is going to be very useful.
SP-084
NANOTECHNOLOGY IN THE PROSPERING MODERN

TEXTILE INDUSTRY
Nisha Arya and Neha Chauhan
Department of Textile and Apparel Designing
I.C. College of Home Science, CCS HAU, Hisar-125004
Email: nishasangwan@gmail.com
Nanotechnology holds an enormously promising future straight line. The new concepts
exploited for the development of nano-finishes have opened up exciting opportunities for
the further research and development. The application of nanomaterial and
nanotechnology is growing at a remarkable rate in all arenas of science and technology
such as chemistry, biology, physics, materials science, agriculture, medical sciences and
engineering. The Textile industry is also experiencing the benefits of nanotechnology in
its varied field of applications.
Nanotechnology has versatile applications in Textile Chemicals industry in
manufacturing garments with stain resistance, flame retardant finishes, wrinkle
resistance finishes, moisture management, easy-clean, antimicrobial qualities, anti-odour,
UV protection, and soil release properties. The future success of nanotechnology in
textile applications lies in areas where new principles will be combined into durable,
multifunctional textile systems without compromising the inherent textile properties,
including process ability, flexibility etc.
The prime aim is to summarize recent applications of nanotechnology in the field
of textiles as they relate to textile fibers, yarns, and fabrics with an emphasis on
improving various properties of textiles. Moreover, it overcomes the limitations of
applying conventional methods to impart certain properties to textile materials, hence,
there is no doubt that in the next few years nanotechnology will penetrate into every area
of textile industry.
SP-085
SMART TEXTILES: ENHANCE THE NATURAL

BEAUTY
Zeba Jamal, Sushma Rani, Vivek Singh and Parveen Punia
Department of Textile and Apparel Designing2
COHS, CCS HAU, Hisar-125004, Haryana
Email: zebajamal22sept@gmail.com
Serviceability of the textiles is the first requisite for the consumer. From primitive
era, textile is used for clothing which was extended to household and domestic purpose
with progressive civilization. Todays textile science stands on a novel, unexplored and a
vision filled sphere. Nanotechnology is increasingly being used in the field of textiles, to
produce fabrics with superior properties and functionalities that are smart, natural and
intelligent. Smart textiles that is able to sense stimuli from the environment, to react and
adapt to them by integration of functionalities in the textile structure. It is an
amalgamation of almost all applied science. In smart textiles, fabrics can be efficiently
produced for different purposes including medical, military, industrial apparels which are
antimicrobial, antistatic, flame retardant, water and oil repellent, wrinkle resistant, selfcleaning properties and UV-blocking etc. Due to increase in awareness about health and
hygiene, people increasingly want their clothing to be hygienically fresh. Therefore,
many of the consumers are want to opting for herbal anti-microbial finishes for textiles.
Due to application of chemical and natural botanical anti-microbial agents microbial
growth on the textiles can be inhibited. Many plant extracts from roots, stem, leaves,
flowers, fruits and seeds of diverse species of plants exhibit anti-bacterial properties.
Use of plant extracts for various textile are safeguards for the environment, prevent
pollution and promote eco-friendly textiles. Guava leaves which are powerhouse of
phenolic compounds possess excellent antioxidant and antimicrobial properties. Guava
leaves extract on cotton fabrics is an effective treatment for bacterial resistance or
develop a smart or natural textile. Advanced materials and polymers indicate that smart
and natural textile materials and their application will boom in the near future.
SP-086
APPLICATION OF NANOTECHNOLOGY IN THE

BLOOMING AGRICULTURE
Neha Chauhan and Nisha Arya
Department of Textile and Apparel Designing, I.C. College of Home Science, CCSHAU, Hisar
Email: chauhan.neha.5492@gmail.com
Textiles are important parts of our lives and the future of clothing is about to transform
enormously as smart textiles are paving the way for this change. The escalating interest in
Nano technology opens a head gate of opportunities for developing new and advanced
products in the textile sector. Nanoscale manipulation results in new functionalities for
intelligent textiles, including self-cleaning, sensing, actuating, stain resistant, self-cleaning,
antistatic,controlled hydrophobicity or hydrophobicity, UV protective, and
communicating.These additional functionalities have numerous applications,
encompassing healthcare, sports, military application and fashion. There are so many
prospective applications where smart nanotextiles may influence on our lifestyles and
become ubiquitous in this technology driven world.The prime aim is to summarize current
research in nanotechnology application to Agrotextiles.
SP-087
SYNTHESIS AND CHARACTERIZATION OF ZINC

OXIDE NANOPARTICLES AND THEIR APPLICATION
IN THE TEXTILES AS UV-ABSORBERS
Pooja, Dr Anil Dhaka
Department of Botany, Pt.N.R.S Govt. College, Rohtak (Haryana)-124001
Email: pj7790@gmail.com
In the textile industry the use of nanotechnology has increased rapidly. This is mainly
because the conventional methods which are used to impart different properties to
fabrics often do not lead to permanent effects and will lose their functions after
laundering or wearing. But Nanoparticles can provide high durability for treated
fabrics with respect to conventional materials because they possess large surface area
and high surface energy that ensure better affinity for fabrics and lead to an increase in
durability of the textile functions. The coating of Nanoparticle affect the fabric
properties like dyeing capacity, tensile strength, bursting strength, bending rigidity, air
permeability (comfort) and fabric friction that play a crucial role in textile industries.
But Zinc oxide is widely used in different areas because of its unique photocatalytic,
electrical, electronic, optical, dermatological, and antibacterial properties. Zinc oxide is
actually one of the best absorbers of UV radiation that mainly come on Earth from the
Sun through the atmospheric ozone layer. Synthesis and characterization of ZnO
nanoparticles obtained through a homogeneous phase reaction between zinc chloride
and sodium hydroxide at high temperature.
SP-088
SELF CLEANING FINISH ON TEXTILES BY USING

NANOTECHNOLOGY
Mona Verma, Saroj S. Jeet Singh
Department of Textile And Apparel Designing,I. C. college of Home Science
Chaudhary Charan Singh Haryana Agricultural University, Hisar
Email: mona.verma35057@gmail.com
Self-cleaning is the term which has attracted lot of attention in the recent years. One way
to achieve self-cleaning properties of textiles is to employ nanotechnologies, especially
metal oxide nanoparticles with photocatalytic activities. A self-cleaning coating
comprised of photocatalytic titanium dioxide (TiO2) offers three unique properties when
exposed to ultraviolet (UV) light: 1) strong oxidation power that eliminates odour
causing bacteria; 2) the breakdown of long chain organic molecules into smaller ones;
and 3) a surface that experiences super-hydrophilicity, which allows these small chained
organic molecules and everyday dirt and stains to be easily washed away with water.
Self-cleaning coatings have the potential for creating important labour-saving and
bacteria resistant surfaces on textiles material. Several studies reveal that the selfcleaning properties of woven cotton fabric after coating with nanoparticles of Tio2 were
studied and it was found that the self-cleaning effect was stronger for samples coated
with higher Tio2 concentrations. The ultimate consequence in developing these selfcleaning fabrics is that we can really limit our use of things like chemicals, energy and
water. That offer superior resistance to dirt as well as water and require much less
cleaning than conventional fabrics. Self-cleaning property will become a standard feature
of future textile and other commonly used materials to maintain hygiene and prevent the
spreading of pathogenic infection by environment benign route. The coating could be
applied to suits, hospital garments, sportswear, military uniforms and outdoor fabrics.
SP-089
AQUACULTURE FOR IMPROVING FISH PRODUCTION
P. Swain, S. S. Mishra, R. Das, S. K. Padhi, K. C. Das, P. Jayasankar
ICAR-Central Institute of Freshwater Aquaculture,
Kausalyaganga, Bhubaneswar-751002
Email: pswainy2k@yahoo.co.in
Nanotechnology involves the application of materials at the nanoscale i.e. 1 to 100nm.

Common nanoparticles used in aquaculture are metal and metal oxides (ZnO, Fe, CuO,
Ag, Au etc), inorganic (Calcium phosphate, MgO, Se, Sulfur etc.) and polymeric
(chitosan, Poly lactide-co-glycolic acid (PLGA), Poly caprolactone (PCL). They are used
in treatment and remedy of water qualities; fish health management including disease
diagnosis, wound healing and immune modulations; control of bacterial biofilm, fungal
infections and algal blooms; keeping quality of fish feed and fish products; enrichment
of micronutrient content of fish flesh; skin coloration and quality of aquarium fishes;
hatchery applications for enhancing hatching and survival of fish fry.
The nanoparticles particularly from metals such as zinc oxide, copper oxide, iron oxide,
aluminium oxide, silver, silver - titanium dioxide, magnesium oxide, gold, selenium and
sulfur nanoparticleswere synthesized indigenously and characterized in our laboratory
through chemical, biological and microbial methods. Nanoparticles such as gold, sulfur,
selenium, silver, silver - titanium dioxide, copper, zinc, iron, TiO2aluminium oxide, and
magnesium oxide have bactericidal and fungicidal activities. Metal nanoparticles such as
zinc oxide, copper oxide and selenium showed algicidal and antialgal properties. These
nanoparticles significantly inhibited algal growth via pore formation and disruption their
plasma membrane, efflux of intracellular components and reduced the chlorophyll
contents.
Dietary administration of Selenium nanoparticles improved the muscle selenium content,
antioxidant enzymatic activities and WBCs content of fish, Labeo rohita. It also upregulated expression pattern of different innate, anti-inflammatory and metabolic stress
genes.Iron nanoparticles supplemented in the basal diet could improve the final weight
and antioxidant enzymatic activities (SOD, MDA, GSH), and haematological and
immunological parameters and muscle iron and hemoglobin content of Indian major
carps, L. rohita.Zinc oxide (ZnO) nanoparticles inhibited the growth of Aspergillus
flavus strains in feed. Moreover, feed supplemented with higher dose of zinc
nanoparticles (10mg/g- 1mg/g) were found to inhibit Pseudomonas sp., Enterobacter sp.
and Erwinia sp.Dietary iron and zinc nanoparticles body influenced body coloration and
carotenoid content of skin with intactness and shining of skin.Nanoparticles such as CuO
and ZnO exhibited highest percentage of hatching in both direct addition and its coating
onto tanks. Further it decreased microbial load of water without affecting water quality
parameters. Considering the beneficial effects of CuO and ZnO nanoparticle application,
it has the scope of being used in a more eco-friendly way in hatchery operations.
SP-090
CONTROLLED RELEASE OF FERTLIZERS

THROUGH NANOTECHNOLOGY
Ravi Kumar T, Srikanth M. and Mamatha N. C.
Department of vegetable science, College of Agriculture, CCSHAU, Hisar (Haryana)
Email: ravikumar.horti@gmail.com
In India from the past 50 years, the fertilizer consumption increased from 0.5 (1960s) to
26.05 million tonnes (2014) with four-fold increase in food grain output (257.07 million
tonnes). The optimal NPK fertilizer ratio of 4:2:1 is ideal for crop productivity while the
current ratio is being maintained at 10: 2.7: 1 in India. Nanotechnology has provided the
feasibility of exploiting nanostructured materials as fertilizer carriers for building of socalled smart fertilizer as new facilities to enhance nutrient use efficiency and reduce
costs of environmental protection. Encapsulation of fertilizers within a nanoparticle can
be done in three ways a) the nutrient can be encapsulated inside nanoporous materials, b)
coated with thin polymer film, or c) delivered as particle or emulsions of nanoscales
dimensions. Fertilizer particles can be coated with nanomembranes that facilitate in slow
and steady release of nutrients. This process helps to reduce loss of nutrients while
improving fertilizer-use efficiency of crops. Nano-fertilizer formulation is capable of
releasing nutrients to the tune of 1000-1200 hrs while conventional fertilizer could
release only up to 300-350 hrs. About 40-70% of nitrogen and 80-90% of phosphorous
of the applied normal fertilizers are lost to the environment. The adsorbents zeolite,
halloysite, montmorillonite and bentonite nanoclays were used for N, P, K and S
micronutrients, mannose and amino acids with controlled release characteristics. Nanocoating of seeds using elemental forms of Zn, Mn, Ag will not only protect seeds but
reduces the requirement of elements to far less quantities than recommended fertilizer
usage. Nano fertilizer nutrients more available to nanoscale plant pores and therefore
result in efficient nutrient use for long run.
SP-091
ROLE OF NANOTECHNOLOGY IN FORESTRY

AND WOOD SCIENCE
Pradyuman Singh, K. S. Bangarwa and Md. SarwareAlam
Department of Forestry,
College of Agriculture, CCS Haryana Agriculture University, Hisar, Haryana-125004
Email: Pradyuman12@gmail.com
Nanotechnology is defined as the manipulation of materials measuring 100-nanometers

or less in at least one dimension (Bruus, 2004). Nanotechnology is expected to be a
critical driver of global economic growth and development of this century. Already, this
broad multi-disciplinary fieldis providing glimpses of exciting new capabilities, enabling
materials, devices and systems that can be examined, engineered and fabricated at the
Nano scale (Roco,2006). Using nanotechnology to controllably produce nanomaterials
with unique properties is expected to revolutionize technology and industry. The forest
products industry relies on a vast renewable resource base to manufacture a wide array of
products that are indispensable to our modern society.
Emerging nanotechnologies offer the potential to develop entirely new approaches for
producing engineered wood and their fiber- based materials. They can also enable the
development of wide range of new or enhanced wood based materials and products that
offers cost effective substitutes for non renewable materials used in manufacture of
metallic, plastics, or ceramic products. Nanotechnology could transform the forest
products industry in virtually all aspect ranging from production of raw materials, to
new applications for composite and paper products, to new generations of functional
Nano scale lignocelluloses. Wood protection applications have been a significant focus
of nanotechnology including the utilization of Nano biocides and Nano carrier delivery
systems in wood preservatives.
SP-092
NANOTECHNOLOGY IN ENVIRONMENTAL SAFETY

Anil kumar*, Sukhdeep Singh Sivia, Reena Rani and Sunayana
Department of Genetics & Plant Breeding
CCS Haryana Agricultural University, Hisar, Haryana (India), 125004
Email: agusaiwal@gmail.com
Nanotechnology relates to materials, systems and processes which operate at a scale of

100 nm or less. Nanotechnology can be applied simultaneously to remove the harmful
effects of highly toxic organic pesticides and increasing the fertility of the soil through
photocatalysis. Nanostructures,nanoparticles and nanowires offer large surface to volume
ratio and attract higher probability of the organic molecules to come in contact with the
metal oxide molecules residing on the surface of the nanoparticles. Owing to high aspect
ratios, nanowires are capable of degrading organic molecules faster than similar amount
of nanoparticles. There are reports on the photocatalysis of herbicides like 2,4-D and
pesticides like tetrachlorvinphos, dichlorvos, phosphamidon and cyproconazole. Theend
productsof photocatalysisare carbon dioxide which escape into the atmosphere, water
and mineral salts which add to the fertility of the soil. The use of photocatalysis for
purification, decontamination and deodorization of air, for the removal of organics,
destruction of cancer cells, bacteria and viruses is becoming popular.Nanotechnology
can offer added value to crops or environmental remediation. Nanotechnology can also
be used to clean ground water e.g. the use of aluminium oxide nanofibres (NanoCeram)
can remove viruses, bacteria and protozoan cysts from water. Nanocheck a commerical
lanthanum nano-particle is utilized for cleaning fish ponds and swimming pools
effectively. The iron nanoparticles catalyse the oxidation and breakdown of organic
contaminants such as trichloroethene, carbon tetrachloride, dioxins, and PCBs to simpler
carbon compounds which are much less toxic. Nanoscale particles can help to develop
potable water problems in the developing world.
SP-093
APPLICATION OF NANOTECHNOLOGY IN WATER

Manju Devi and Sudesh Rani
Department of Zoology, Maharshi Dayanand University, Rohtak- 124001 Haryana, India.
Email: sudesh_zoology@gmail.com
Today nanoparticles, nanomembrane and nanopowder used for recognition and removal
of chemical and biological substances include metals (e.g. Cadmium, copper, lead,
mercury, nickel, zinc), nutrients (e.g. Phosphate, ammonia, nitrate and nitrite), cyanide,
organics, algae (e.g. cyanobacterial toxins) viruses, bacteria, parasites and antibiotics.
Basically four classes of nanoscale materials that are being assayed as functional
materials for water purification e.g. metal-containing nanoparticles, carbonaceous
nanomaterials. In the vicinity of water purification, nanotechnology offers the prospect
of an efficient removal of pollutants and germs. The incorporation of engineered
nanoparticles into drinking water treatment technologies against the removal of heavy
metals, microorganisms and organic pollutants appears as a very dynamic branch of
nanotechnology. Nanoparticles known for their potential to the high specific surface area
and surface reactivity compared to conventional bulk materials.The most important is
their availability in the required large quantities and their efficiency to fulfil the strict
regulations for drinking water consumption and environmental safety.
A REVIEW ON NANOTECHNOLOGY FOR

SUSTAINABLE AGRICULTURE
SP-094
Mamta Nehra1*, R. K. Sharma2 and R. K.Yadav2

1
GB Pant University of Agriculture and Technology, Pantnagar-263145

2
ICAR-Indian Agricultural Research Institute, New Delhi-110012
Email: mamtanehra089@gmail.com
Nanotechnology applications in the agriculture and food sector in agriculture, technical

innovation is of importance with regard to addressing global challenges such as
population growth, climate change and the limited availability of important plant
nutrients like phosphorus and potassium. The application of nanomaterials in agriculture
aims in particular to reduce applications of plant protection products, minimize nutrient
losses in fertilization, and increase yields through optimized nutrient management.
Nanotechnology devices and tools, like nano capsules, nanoparticles and viral capsids,
are examples of uses for the detection and treatment of diseases, the enhancement of
nutrients absorption by plants, the delivery of active ingredients to specific sites and
water treatment processes. The use of target-specific nanoparticles can reduce the
damage to non-target plant tissues and the amount of chemicals released into the
environment. Nanotechnology derived devices are also explored in the field of plant
breeding and genetic transformation. The transfer of genes to the target plant cells has
been accomplished through the use of a variety of nano tools, including nanoparticles
that encapsulate and deliver DNA to target cells. Nanosensors have also relevant
implications for application in agriculture, in particular for soil analysis, easy biochemical sensing and control, water management and delivery, pesticide and nutrient
delivery. Many production processes are being developed nowadays to obtain useful
nanocomposites from traditionally harvested materials. The potential of nanotechnology
in agriculture is large, but a few issues are still to be addressed, such as increasing the
scale of production processes and lowering costs, as well as risk assessment issues.
SP-095
TOWARDS ADOPTING NANOTECHNOLOGY FOR

FERTILIZERS: ORGANIC FERTILIZERS
Amandeep Kaur, Shweta, Renu Bala and Anurag
Department of agronomy, college of agriculture, CCSHAU, HISAR
Email: ama.in.pau.@gmail.com
Fertilizer plays a pivotal role in agriculture production (35 to 40%). To enhance nutrient
use efficiency and overcome the chronic problem of eutrophication, nanofertilizer might
be a best alternative. Slow controlled release fertilizers may also improve soil by
decreasing toxic effects associated with fertilizer over- application. FYM, vermicompost
or organic fertilizers are ultimate slow-release fertilizers, and can work as nano
fertilizers. Slow-release fertilizers involve a slower release rate of nutrients than
conventional water-soluble fertilizers, in which the rate, pattern, and duration of release
are not controlled.
Nanofertilizers are synthesized in order to regulate the release of nutrients depending on
the requirements of the crops, and it is also reported that nanofertilizers are more
efficient than ordinary fertilizer (Liu et al., 2006a). Nanofertilizers could be used to
reduce nitrogen loss due to leaching, emissions, and long-term incorporation by soil
microorganisms. They could allow selective release linked to time or environmental
condition. Slow controlled release fertilizers may also improve soil properties by
decreasing toxic effects associated with fertilizer over- application (Suman et al., 2010).
These can be easily incorporated in various media such as gels, creams, liquids among
others, and have multiple applications for preventative measures, treatment or
preservation of harvested products.
SP-096
NANOBIOTECHNOLOGY: REVOLUTIONIZING
THE WORLD
Vinod Kumar, S.K. Tehlan, Ankush, Amit Kumar
Department of Vegetable Science, Chaudhary Charan Singh Haryana agricultural University, Hisar,
Haryana
Email: vinoddudhyan68@gmail.com
Nanotechnology is often described as an emerging technologyone that not only holds

promises for society, but also is capable of revolutionizing our approaches to common
problems. Nanotechnology is not a completely new field; however, it is only recently
that discoveries in this field have advanced so far as to warrant examination of their
impact upon the world around us. The strength of spinach isn't only in its nutrients, but
also in its ability to be hacked to function as a sensor. A group of engineers from
Massachusetts Institute of Technology led by Michael Strano has converted ordinary
spinach plants into biological bomb detectors. The engineers implanted customised
carbon nanotubes underside the leaves of spinach by using the technique called vascular
infusion which turn them into a real-time monitoring system for explosive molecules.
When the plants suck water from the ground into the leaves, the carbon nanotubes can
detect the presence of any nitroaromatics chemical compounds often found in
explosives such as landmines. When the researchers shine a laser on the nanotubes, they
emit a fluorescent signal if they pick up nitroaromatics. This signal can be detected by an
infrared camera up to a metre away. The research, published in Nature Materials, has yet
to be tested for real, but eventually it could be possible to sow seeds across a site
suspected of containing landmines and use the plant detection system to locate them.
SP-097
OPTIMIZATION OF CONDITIONS FOR

DELIGNIFICATION OF PADDY STRAW
Anita, Kamla Malik and Shikha Mehta
Department of Microbiology, College of Basic Sciences and Humanities,
CCS Haryana Agricultural University, Hisar-125004 (Haryana), India
Email: kamlamalik06@gmail.com
Lignin is one of the most abundant aromatic natural substances present in the biosphere.
It is a polymer composed of phenylpropanoid units, associated with cellulose and
hemicelluloses in plant cell. In biological treatment systems, a wide variety of
microorganisms including fungi, actinomycetes and bacteria have been used in lignin
biodegradation. Now a day, the ligninolytic bacteria gaining importance, because of
wider tolerance of temperature, pH and oxygen limitation than fungi. In the present
study, a total of 15 bacterial isolates were obtained from soil and different ecological
niches and screened for ligninolytic activity. Among these, five bacterial isolates were
used for the delignification of paddy straw with different conditions like temperatures
(25, 30 and 35C), pH (5.0, 6.0 and 7.0) and incubation period (7, 14 and 21 days) under
stationary condition. Optimum temperature for delignification was found to be 30C and
pH 6.0 for DS2 and LS1 whereas pH 7.0 for CS3, CS4 and PS1 after 21 days of
incubation. All the bacterial isolates grew better and showed maximum degradation of
lignin at 300C as compared at 25 and 350C. The maximum lignin removal (32%) was
observed by isolate DS2 followed LS1 (30%), PS1 (29%) after 21 days).
Delignification was associated with bacterial isolates change in color and decrease in
weight and texture as compared to untreated paddy straw.
SP-098
NANOPARTICLES: A TOOL FOR PESTICIDE

REMEDIATION
Anil Duhan*, Makhan Lal1, Dinesh Tomar2, S.S. Punia, Ritu3 and Bunty Sharma3
Department of Agronomy, 1Department of Vegetable Science, 2Department of Soil Science, 3Department

of Chemistry and Bio-chemistry,
Email: a.duhan@rediffmail.com
With the advancement of industrialization, agricultural and urban activities the levels of
groundwater pollution have increased many folds in the last few decades. Due to it, there
is an increase in the concentration of many organic and inorganic pollutants much more
above the permissible limits of drinking water standards. Moreover extensive use of
pesticides in India has also led to the increase in the pollutants concentrations in
groundwater. The most harmful compounds that are finding their ways in groundwater
through various illegal industrial and agricultural activities includes various
organochlorine & organophosphorous pesticides, heavy metals and other carcinogenic
compounds. The application of nanotechnology for the remediation of contaminants may
give promising results in future. In recent years, a great deal of attentions has been
focused onto the application of nanostructured materials as adsorbents or catalysts to
remove toxic and harmful substances from wastewater and air. Reactive nanoparticles
appear to be useful in remediating groundwater and thus may prove useful in removing
pesticides and herbicides in the environment. Nanoparticles also may provide a more
efficient and controlled delivery/release method for the application of pesticides and
fertilizers. Iron nanoparticles and their derivatives provide a potential for more effectual
and inexpensive substitute to many remedial technologies. The particle small size of the
iron nanoparticles (1-100 nm) facilitates a very high level of remedial flexibility. The use
of nano ZVI, bimetallic nanoparticles, and emulsified zerovalent nanoparticle forms the
whole sole for both soil and groundwater remediation. The most basic form of the nano
iron technology encompasses nano ZVI and reactive nanoscale iron product (RNIP). The
practical applicability of these nano zero valent iron (Fe0) particles lies in the fact to get
oxidized into +2 and +3 oxidation states thereby reducing other organic as well as
inorganic impurities.
SP-099
SUSTAINABLE FARMING THROUGH

NANOTECHNOLOGY WITH SMART
AGRICULTURAL PRACTICES
Anil Kumar Rohila, P.S. Shehrawat, Ansul1, B.S. Ghanghas and Krishan Kumar
Department of Extension Education, CCSHAU, Hisar-125004
1
Department of Vegetable Science, CCSHAU, Hisar-125004
Email: rohillaextension@gmail.com
Agriculture is an important sector in India, it not only contributes to the national income
but also provides livelihood to about two-third of the population of the country. In the
worlds scenario, Indian economy is growing at faster rate and agriculture is major sector
that contributes in our economy. Agricultural production is unstable till now because of
its total dependence on weather and natural biological uncertainties in managing crops.
With the population growth rate at 1.58 per cent, India is predicted to have more than
1.53 billion people by the end of 2030 and natural resources such as land, water, forests,
livestock, fisheries is degrading fast due to unmindful agricultural intensification,
imbalanced use of fertilizers, misuse and inefficient use of irrigation water, deforestation
and climate change. So we need Smart Agricultural Practices (SAP) such as
nanotechnology, INM, IPM, IWM, IFS, protected cultivation and organic farming etc to
increase production, productivity, benefit and employment along with especially for
sustainable use of natural resources and environmental friendly farming. Continuous
increasing world population, it is necessary to use the SAP such as nanotechnology in
agricultural and food sciences. So, it can be concluded that Nanotechnology with SAP
applied to agricultural production could play a fundamental role for this purpose.
SP-100
APPLICATIONS OF NANOTECHNOLOGY
IN AGRICULTURE
Divya Chanchal and Ravi Mehndiratta
Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural

University Hisar
Email: divya.march6@gmail.com
More than 60% of population in developing countries relies on agriculture for survival.
Plant breeders and scientists have to face many challenges such as low crop yield,
decline in soil organic matter, poor soil health, sudden climate changes etc in order to
feed tremendously increasing population and decreasing cultivable land. We need to
attain sustainable growth in agriculture. Nanotechnology has emerged as a fascinating
field to mitigate these problems. It deals with matter at nano scale (1-100nm)
dimensions. Working at nano scale provides unique physical properties to materials
which are not shown by bulk materials. These properties can be utilised to develop
powerful products and processes in various fields.
Talking about agriculture, some of the applications of nanotechnology includes nanocapsules for delivering pesticides, fertilizers and other agrichemicals more efficiently ,
nano-sensors for monitoring soil condition, crop growth and detection of animal and
plant pathogens, nano-chips for identity preservation and tracking, nano-particles to
deliver DNA to plants( nano-biotechnology), encapsulated nano-herbicides to improve
the efficacy of herbicides, nano soil binder,SoilSet to avoid soil erosion problems.
Nano biotechnology has designed sensors with increased sensitivity and earlier response
to environmental changes that are linked to GPS. Research on the applications of
nanotechnology in agriculture is less than a decade old and it may take few more years to
get transferred from lab to land.
SP-101
A REVIEW ON NANOTECHNOLOGY AND

ITS IMPLICATIONS IN SEED SCIENCE AND
TECHNOLOGY FOR QUALITY SEED
Rajesh Kumar Sharma1*, Mamta Nehra2 and Bharat Taindu Jain3
1
ICAR-Indian Agricultural Research Institute, New Delhi-110012

GB Pant University of Agriculture and Technology, Pantnagar-263145
3
Email: sharma.rajesh089@gmail.com
High quality seed is the basic and critical input that acts as key factor for successful
agriculture. Modern agriculture with its bias for technology and precision, demands that
each and every seed should readily germinate and produce a vigorous seedling ensuring
higher yield. Many scientists all over the world have developed many new production
techniques called seed enhancement techniques viz., seed polymer coating, seed
colouring, seed pelleting, seed fortification, seed infusion, etc. Nanotechnology, the
science of working with smallest possible particles, raises hopes for the future to
overcome the difficulties encountered in agriculture. Recent manufacturing
advancements have led to the fabrication of nanomaterials of different sizes and shapes.
These advancements are the base for further engineering to create unique properties
targeted toward specific applications. With new nano-scale techniques of mixing and
harnessing genes, genetically modified plants become atomically modified plants. In
Seed Science Research, nanotechnology offers the tools like various nanoparticles for
improvement of seed germination and related physiological parameters, nanomembranes
and nanopolymer coating to enhance the storability of the seeds by incorporation of
pesticides, nanosensors for better management of seed infestation during storage. Visions
of an automated, centrally-controlled industrial agriculture can now be implemented
using molecular sensors, molecular delivery. The agricultural industry is no exception.
So far, the use of nanotechnology in agriculture has been mostly theoretical, but it has
begun and will continue to have a significant effect in the areas of seed science. This
review summarizes applications of nanomaterials in seed science and the role these can
play in future agricultural production.
SP-102
POTENTIAL APPLICATIONS OF NANOPORE

SEQUENCING
Rinku Chaudhary
Email: rinkupilania033@gmail.com
DNA sequencing is the most powerful method to reveal genetic variations at the
molecular level, such as single nucleotide polymorphism, copy number variation, gene
fusion, and insertion/deletion, etc., which are relevant to genetic diseases including
cancer. Most current DNA sequencing methods are based on either chemical cleavage of
DNA molecules or synthesis of new DNA strands. Alternative of this is using nanopore
sequencing. Nanopore based DNA sequencing involves threading single DNA strands
through tiny pores in a membrane. The advantages of this approach include potentially
very long and unbiased sequence reads as no amplification or chemical reactions are
necessary. Also a pore-based instrument could be very inexpensive, sequencing would
occur as fast as the molecules can pass through the pores. The ability to distinguish
between purine and pyrimidine strands in a single RNA molecule generated growing
interest in nanopore analysis of nucleic acids. Nanopores can also be used to probe subtle
changes to the internal structure of nucleic acids. The -HL nanopore is able to detect 2,
4, 6-trinitrotoluene (TNT) in an aqueous field following the introduction of aromatic side
chains at position 113 of the -HL polypeptide using site-directed mutagenesis. Also E.
coli genome has been assembled de novo with 99.4% accuracy using MinION alone.The
single-molecule techniques used by this technology allow us to further study the
interaction between DNA and protein, as well as between protein and protein. Further,
nanopores are on the verge of delivering new technologies that will undoubtedly improve
health, the most important of them being DNA sequencing.
SP-103
VEGETABLE PRODUCTION UNDER PROTECTED

CULTIVATION WITH NANOTECHNOLOGY
Ansul*, A.K. Rohila1, Devashri Maan, V.K. Batra, P. Kumar2 and Amit Kumar
Department of Vegetable Science, 1Department of Extension Education, 2Department of Agricultural

Economics, CCSHAU, Hisar-125004
Email: duhanhau33@gmail.com
This article reviewed vegetable production under protected cultivation with

nanotechnology. Vegetables are the main source of vitamins and minerals. The
importance of vegetables in providing balanced diet and nutritional security has been
realised world over. Vegetables play a major role in the agrarian and developing
economy like India. Protected cultivations contribute significantly in agricultural and
national income. Vegetable production under protected conditions is becoming an
essential practice of modern agricultural production in light of a growing world market
demand for off-season and year-round vegetable availability. It helps to achieve better
and higher quality yields otherwise unachievable under open field conditions and enable
vegetable production in areas or seasons that would not normally and nanotechnologies
are hoping that nanotechnology will transform the entire food industry bringing about
change in the production, processing, packaging, transportation and consumption of
food. Usage of nanotechnology to ensure safety of food product, create a health food
culture and enhance the nutritional quality of food. It can be concluded that demand of
vegetable in the world can be fulfilled with the help of protected technology and
nanotechnology.
AGRICULTURAL NANOTECHNOLOGY:
PERMISSIVE SCIENCE FOR CROP YIELD IMPROVEMENT
UNDER DROUGHT STRESS
SP-104
Rajkumar, Kirpa Ram, Pooja Rani, Sapna and Kamal Dutt Sharma
Department of Botany and Plant Physiology, CCS Haryana Agricultural University, Hissar-125004
Email: rajdhankhar87@gmail.com
Abiotic stresses like drought, temperature extremes, floods, salinity, acidity, mineral
toxicity, and nutrient deficiency are threatening agricultural, livestock, fisheries and
others production globally posing challenges in sustaining even the current productivity
levels, let alone further enhancement. Agriculture is an area where new technologies are
often applied to improve the yield of crops. Agricultural nanotechnology involves the
employment of Nanoparticles in agriculture these particles will impart some beneficial
effects to crops. Materials with a particle size less than 100 nm in at least one dimension
are generally classified as nanomaterials. Nanoparticles can be synthesized from metal or
metal oxide through various approaches i.e physical, chemical and biological. In the
current scenario nanoparticles can be a potential tool to be affectively used as plant
growth and development promoters, herbicides, nano-pesticide, nanofertilizers etc.
which can effectively release their content in required quantity to target cellular
organelles in plants. Potential applications of nanotechnology in agriculture are the
following: delivery of nanocidespesticides encapsulated in nanomaterials for controlled
release; stabilisation of biopesticides; slow release of nanomaterialassisted fertilisers,
biofertilisers, and micronutrients for efficient use; and field applications of
agrochemicals, nanomaterials assisted delivery of genetic material for crop
improvement.
SP-105
NANOTECHNOLOGY IN FOOD INDUSTRIES

Pinky Sharma and Meenakshi Rathi
PAU Ludhiana, CCS-HAU, Hisar
Email: meenakshirathi55@gmail.com
Nanotechnology (nanotech) is manipulation of matter on an atomic, molecular, and

supramolecular scaleNanotechnology is the manipulation or self-assembly of individual
atoms, molecules, or molecular clusters into structures to create materials and devices
with new or vastly different properties. Nanotechnology can work from the top down or
the bottom up. Nanotechnology will transform the entire food industry, changing the way
food is produced, processed, packaged, transported, and consumed.Nanotechnology
offers higher hopes in food packaging by promising longer shelf life, safer packaging,
better traceability of food products, and healthier food. Intelligent, smart, and active
packaging systems produced by nanotechnology would be able to repair the tears and
leakages, and respond to environmental conditions. Nanotechnology can provide
solutions for food packaging by modifying the permeation behaviour of foils, increasing
barrier properties, providing antimicrobial properties, and by improving heat-resistance
properties.Nanosensors can provide quality assurance by tracking microbes, toxins, and
contaminants throughout food processing chain through data capture for automatic
control functions and documentation. Grain quality monitoring nanosensors use
conducting polymer nanoparticles which respond to analytes and volatiles in food
storage environment and thereby detect the source and the type of
spoilage.Nanoencapsulation is defined as a technology to encapsulate substances in
miniature and refers to bioactive packing at the nanoscale range. Thus,
nanoencapsulation has the potential to enhance bioavailability, improve controlled
release, and enable precision targeting of the bioactive compounds.Nanocaosules are
capable of changing foodcolor, flavor, or nutritional properties according to a persons
dietary needs, allergies, or taste preferences are on the research.Nanotechnology is
becoming increasingly important for the food sector. Nanotechnology has begun to nd
potential applications in the area of functional food by engineering biological molecules
toward, opening up a whole new area of research and development.
SP-106
MONITORING YELLOW RUST INFECTION OF

WHEAT USING NANO-SENSORS
Reema Rani, Rizwana Rehsawla, Neelam R. yadav
Department of Molecular Biology, Biotechnology and Bioinformatics, CCSHAU Hisar-125004, Haryana

Email: reemasherwal@gmail.com
Wheat stripe rust or yellow rust caused by Puccinia striiformis Westend. f. sp. Tritici
Eriks, is a serious threat to the wheat production worldwide. This polio of agriculture,
spread by releasing billions of spores in the wind which can cause massive crop failures
within a few weeks. Considering that the occurrence of yellow rust on wheat depends on
cooler temperature and high humidity, a simple, accurate and sensitive sensing technique
is required for early forecasting to protect the wheat crop from the possible outbreak of
rust and to closely monitor the severity of this disease. With the advent of high
throughput nanotechnology, nano based biosensor provides friendly platform for the
assembly of bio-recognition element, the high surface area, high electronic conductivity
and plasmonic properties of nanomaterials that enhance the limit of sensing. Nanosensors can be coupled into a GPS system for real-time monitoring the field to monitor
crop conditions and to closely monitor the yellow rust inoculum in air, plant health and
growth. Nano-sensors based forecasting can allow an earlier response to yellow rust
infection in the crop and stop the epidemic of yellow rust into the wheat crop fields. This
way farmer can be assisted in the earlier identification of hot-spots of rust in the wheat
fields, reduction of inputs and right-crop recommendation can be given to them.
Nanosensors based monitoring and assessment of the spatial distribution of rust disease
in fields would also greatly assist both spray decision making and spray application. This
technology can encourage the diffusion of precision agriculture for an efficient
management of resources and energy.
SP-107
NANOTECHNOLOGY BASED BIOSENSORS:

A DIAGNOSTIC TOOL FOR DETECTION OF PLANT
PATHOGENS
Basavaraj. K, A.S. Rathi, Anil Kumarand Gurav N.P
Department of Plant Pathology, CCS HAU, Hisar (Haryana)
Email: basavaraj4229@gmail.com
Considering the economic losses in agricultural production caused by plant diseases,

monitoring and detection of diseases, can facilitate the management of diseasesin time.
Widely used detection methods are real-time polymerase chain reaction, microarray and
enzyme-linked immunosorbent assays (ELISA) that can be highly sensitive and specific,
but they often require extensive sample cleanup and biomolecule purification.In this
context, biosensors show promising potential due to their speed of analyses as well as
label-free detection of pathogens. A biosensor is analytical device rely on the intimate
coupling of a biological recognition element with a physical transducer to convert the
biological signals into a electrical signal or other signals, proportional to the
concentration of analytes. A basic unit of biosensor includes a receptor, transducer and
processor. The sensing elements may be enzymes, antibodies, nucleic acids or whole
cells forming a recognition layer that is integrated with transducer via immobilization.
Transducers are based upon the parameters of measurement, which may be
amperometric, potentiometric, piezoelectric, thermal or optical. Adsorption,
encapsulation, matrix entrapment, covalent binding and cross linking are the major
immobilization methods used to achieve close contact between bioreceptor and
transducer. A biosensor based on surface plasmon resonance (SPR)for the selective
detection of Maize chlorotic mottle virus (MCMV) with detection limit of 1 ppb, which
is of two order lower magnitude than that of existing ELISA method and A quartz crystal
microbalance (QCM) immunosensor for the detection of two orchid viruses, Cymbidium
mosaic virus and Odontoglossum ringspot virus by pre-coating the QCMs with virusspecific antibodies, able to detect as low as 1 ng has been developed and demonstrated.
Nano particle based biosensors are specific, sensitive, rapid and economical, thus
providing a viable alternative to plant pathogen detection methods.
SP-108
DNA ASSEMBLY AT NANOSCALE FOR VARIOUS

APPLICATIONS
Priti Saini1, Vrantika Chaudhary1, Umang Ahlawat2, Rekha Sansanwal2 and Anu Naruka3
Department of Molecular Biology, Biotechnology and Bioinformatics, 2Department of Microbiology

3
Department of Genetics and Plant Breeding, CCS Haryana Agricultural University Hisar
Email: preetykapoor01@gmail.com
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for
technological uses. DNA molecules beyond their purpose as carrier of genetic
information could be used to build two and three dimensional rationally designed
objects with prescribed size, shape, and function at nanoscale. DNA nanotechnology
uses the unique molecular recognition properties of DNA and other nucleic acids to create
self-assembling branched DNA complexes with useful properties. DNAzymes, aptamers
and aptazymes are collectively called functional DNAs, whose functions extend beyond the
WatsonCrick base pair recognition of complementary strands control and can be used to
ne-tune the structure and dynamics of DNA. There are DNA based nanowires,
nanoarchitectures, computers, biosensors, aptamers, biocatalysts, devices and machines. In
these works, the characteristics of DNA including facile synthesis by the solid phase
method, self assembly, electro-conductivity, information elements, amplification,
switching, molecular recognition and catalytic functions were appropriately applied. Gold
Nanoparticles and DNA complexes have been investigated for DNA sequencing and
protein detection. Area of DNA nanotechnology has been signicantly broadened, and the
DNA nanomaterials and devices are useful into many other elds for practical applications,
such as sensing, environmental monitoring, medical diagnostics, drug screening,
therapeutics, nanoelectronics, nanophotonics, and quantum computing. However the
application of DNA molecules is still under study because of big gap that exists between
theory and practice. The future is anticipated a coming out of DNA due to breakthroughs in
nanobiotechnology.
SP-109
AGRONANOTECHNOLOGY: PROMISING UPSHORT

OF AgNPs AGAINST PLANT PATHOGEN
Disha Kamboj and Sumit Jangra
Department of Molecular Biology, Biotechnogy & Bioinformatics, CCS HAU Hisar
Email: dishakamboj5@gmail.com
Nanotechnology aims to explore the biological properties of already known

antimicrobial compounds by manipulating their size. Traditional strategies like
integrated pest management used in agriculture are insufficient, and application of
chemical pesticides have adverse effects on animals and human beings apart from the
decline in soil fertility. Therefore, nanotechnology would provide green and efficient
alternative for the management of insect pests in agriculture without harming the nature.
Different nanomaterials like copper, zinc, titanium, magnesium, gold, alginate and silver
have been developed, but silver nanoparticles (AgNPs) have proved to be most effective
as they exhibit potent antimicrobial efficacy against bacteria, viruses and eukaryotic
micro-organisms. The effects of AgNP-induced plant morphological and physiological
changes showed that AgNPs at lower concentrations exhibit growth-stimulating activity,
whereas at higher concentrations exhibits growth-suppressing activities. The increased
reactive oxygen species (ROS) generation, malondialdehyde production, anthocyanin
biosynthesis, and decreased chlorophyll content were also present at higher
concentrations of AgNPs. The molecular responses of AgNPs indicate that most of the
genes related to secondary metabolism and antioxidant activities were induced at higher
concentrations of AgNP treatment. So, AgNPs can be used as antifungal compounds in
controlling plant diseases in the fields and in turn can promote the overall plant growth.
The application of silver nanoparticles would open a vista of research in integrated
manner such as pathogen control, plant-pathogen interactions, and integrated pest
management.
SP-110
NANOTECHNOLOGY: APPLICATIONS IN FOOD

Meenu Roperia, Anju Kumari, Kritika and Mohammad Sadiq
Centre of Food Science and Technology, CCS Haryana Agricultural University
Hisar-125 004, Haryana, India
Email: roperiameenu@gmail.com
Nanotechnology is one of the most exciting and rapidly evolving fields in agriculture and
food science. Engineered nanomaterials are used as a strategy to harness a controlled
delivery system for food ingredients and additives in processed food. Smart delivery of
nutrients, bioseparation of proteins, rapid sampling of biological and chemical
contaminants and nanoencapsulation of nutraceuticals are some of the emerging
applications of nanomaterials in food. Nanotechnology covers many aspects of food
industry including development of new and improved food packaging materials
(coatings,edible films, materials with superior barrier and mechanical properties);
advanced food security devices (nanosensors, nanotracers); food products with
prolonged shelf life , new tastes , textures and health benefits (e.g. improved mouthfeel ,
beverages with built-in triggered release of colors and flavours); encapsulation systems
that enable delivery of bioactives (probiotics,polyphenols). The concept of nano and
microdelivery systems can offer the benefit of improving the absorption, uptake and
bioavailability of nutrients, it also has the potential to alter the distribution of substances
in the body. Several critical challenges, establishing optimal intake levels, developing
adequate food delivering matrix and product formulation including the safety of the
products can be potentialy improved with the use of engineered nanomaterials.
NEMATICIDAL EFFECT OF PLANT EXTRACT

ON MORTALITY OF ROOT-KNOT NEMATODE
MELOIDOGYNE INCOGNITA UNDER IN-VITRO CONDITIONS
SP-111
Jaydeep Patil, Saroj Yadav and K.K. Verma

Department of Nematology, CCS HAU, Hisar
Email: rajhau99@gmail.com
Investigation was carried out to identify the nematicidal activity of plant leaf origin on
mortality of Root-knot Nematode Meloidogyne incognita. The aqueous extracts from
fresh leaves of Bougainvillea spectabilis were studied for their mortality in various
concentrations viz., 5, 10, 20 and 40 per cent w/v which were prepared with absolute
alcohol and untreated check is also maintained. The mixture was allowed to settle for 48
hour at room temperature and was subsequently passed through filter paper. These semisolid materials were diluted by adding distilled water. The hundred second stage juvenile
of M. incognita were kept in glass cavity blocks containing concentrations of aqueous
extract. The nematicidal activity of leaf extract showed increase in mortality as the
increased concentrations of leaf extract respectively. Increase in juvenile mortality with
the increase in the concentrations of active principle was recorded. These concentrations
also significantly increase mortality of root knot nematode. Among all the different
concentrations of leaf extract the most increase in mortality was occurred at 40 percent
concentration B. spectabilis (80%) mortality were recorded after five days interval. The
no mortality was recorded in untreated check.
SP-112
APPLICATION OF NANOPARTICLES IN MITIGATING

ABIOTIC STRESSES IN PLANTS
Sunita1, Kirpa Ram1, S.S. Dhanda2 and Renu Munjal2
1
Department of Botany & Plant Physiology, 2Department of Genetics & Plant Breeding
CCS Haryana Agricultural University, Hisar, Haryana
Email: k.sunitasangwan@gmail.com
Nanoparticles commonly referred as nano-scale particles having dimensions between 1

and 100 nm. Scientific community has a major concern to alleviate the effect of abiotic
stresses by using these nanoparticles in order to increase the yield potential of crops. In
this concern a new era of technology is emerged named nanobiotechnology, which is
occupying the promising position to mitigate the constraints associated with abiotic
stress to obtain a sustainable and secure future of agriculture worldwide. Nanoparticles
like carbon nanotubes and nanoparticles of Au (gold), SiO2 (silicon oxide), ZnO (zinc
oxide), and TiO2 (titanium oxide) reported to enhance the plant development through
metal nutrient uptake. Along with improving crop yield by increasing mineral
availability these nanoparticles also improve the crop yield by alleviating the heavy
metal toxicity, UV-B induced stress and other abiotic stresses. So these can also be used
in recombinant inbreed lines of bread wheat developed from WH1021WH711 for
improving heat tolerance. Studies in maize suggested that increase in fresh shoot weight
under salinity stress when applied by nano SiO2. Application of silicon on two sorghum
cultivars having different drought susceptibility showed improved drought tolerance
irrespective of their drought susceptibility by lowering shoot to root (S/R) ratio. Nitric
oxide (NO) is a signaling molecule involved in plant response to various abiotic stresses.
Chitosan nanoparticles containing S-nitroso-mercaptosuccinic acid allowed a sustained
NO release and provide efficient protection to plants under salinity. For reducing the
possible negative impacts on crops and natural ecosystem there should be knowledge
about plantnanoparticles interaction, size and concentration before practical
applications in the fields.
SP-113
APPLICATIONS OF NANOTECHNOLOGY IN
HEALTHCARE SECTOR
Kanika Rani
Email: kvats54@gmail.com
Nanotechnology as being the combination of science and engineering at nanoscale, has

great capability in all the three key aspects of healthcare sector-Diagnosis, Prevention
and Treatment. It can revolutionize healthcare sector for the next generation. It can help
in sorting out most agonizing medical issues like repairing of damaged organs, treatment
of cancer, removal of obstruction in brain, recognition of vascular endothelial
dysfunction etc. This technology provides better alternatives for drug delivery systems
for various diseases like arterial plaque, diabetes, HIV etc; measuring hundreds of
complicated factors simultaneously, avoiding the risk of losing samples, waiting days for
lab results and other lab mess-ups. Nano filters are able to remove the smallest of known
viruses. Antimicrobial activity by nanoparticles (NPs) is another promising area for
preventing diseases. This technology provides controlled manufacturing system at the
atomic and molecular level, so structural constraints can be eradicated during drug
designing and the toxic parts of drugs can be eliminated for making them more effective.
It can be used for both in vivo and in vitro biomedical research and applications. NPs can
be used in targeting tumor cells at initial stage. Thus, nanotechnology will serve as
multifunctional tool that can be used with any number of diagnostic and therapeutic
agents with treating diseases.
SP-114
HERBAL ANTIMICROBIAL TREATMENT ON

COTTON FABRIC
Puspa, P. Punia and V. Singh
Department of Textile & Apparel Designing, COHS,
CCS Haryana Agricultural University, Hisar 125 004, Haryana, India
Email: ptomer07@gmail.com
Nanotechnology also has real commercial potential for the textile industry. This is
mainly due to the fact that conventional methods used to impart different properties to
fabrics often do not lead to permanent effects, and will lose their functions after
laundering or wearing. The properties imparted to textiles using nanotechnology include
water repellence, soil resistance, wrinkle resistance, anti-bacteria, anti-static and UVprotection, flame retardation, improvement of dyeability and so on. As there are various
potential applications of nanotechnology in the textile industry. Bacteria grow very
rapidly under warmth and moisture. They damage the textile substrate and also may
promote skin contamination and inflammation in sensitive people. Therefore, there is a
need to inhibit the microbial growth on textile substrate. Antibacterial property of the
textiles reduces microbial colonization as well as adds therapeutic value to the material.
Anti-microbial textiles will improve functionality of textile products, especially when
garments worn close to the skin and also in several medical applications, such as
infection control and barrier material. Cotton fabrics are generally worn next to skin
hence direct contact of textiles with human body provides warmth, humidity and
nutrients. Many plant extracts from roots, stem, leaves, flowers, fruits and seeds of
diverse species of plants exhibit antibacterial properties. For imparting anti-bacterial
properties in this paper the karanja seeds were used which are available in abundance
and underutilized may be found effective for treatment of resistance against microorgasms on cotton fabric. Karanja methanolic extract from seeds in viscous was
prepared by soxhlet method and impregnated in 2.5g/l concentration. The antiseptic
containing chloroxylenol treatment was taken as standard. The effect of karanja seeds
extract treatments and antiseptic containing chloroxylenol (standard) treatment, were
tested for Pseudomonas bacteria resistance activity, quantitatively by AATCC-100 test
method. Bacteria count of treated samples was determined in percent reduction. It was
found hundred percent reductions after 30 minutes of inoculation of Pseudomonas
bacteria. It concluded that seeds extract in viscous state showed comparable results with
the antiseptic containing chloroxylenol (standard).
SP-115
EVALUATION OF INSECTICIDAL PROPERTIES OF

SUPERCRITICAL (GREEN TECHNOLOGY BASED)
EXTRACTS OF BOTANICALS AGAINST INSECT-PESTS
Pallavi Ghosh and D.C. Sharma
Department of Entomology, CSK HPKV, Palampur
Email: sharmadc3@rediffmail.com
A novel, organic solvent free (green extraction) procedure was developed for isolation
and separation separation of bioactive compounds from botanicals. In this procedure
supercritical CO2 was used to obtain extracts from drupes of Melia azedarach L. , leaves
of Eupatorium adenophorum Spreng and Sarcococca pruniformis Lindl. Extraction was
carried out at four different pressures ranging from 75-150 bar. Results shows that rise in
pressure leads to an increase in the bioactive compounds yield of all the three botanicals.
At 150 bar pressure, maximum yield of 5.54 g was obtained in case of E. adenophorum
followed by S. pruniformis (2.83 g) and M. azedarach (1.86 g), respectively from 1kgdry
material. The insecticidal activity of botanical extracts obtained at four different
pressures was evaluated against three important pests of cauliflower (Brevicoryne
brassicae, Pieris brassicae and Plutella xylostella). The LC 50 was worked out to
determine their efficacy against above insects and safety to beneficial insects. In this
paper LC50, LC90 and safety index and safety to environment will be dicussed.
SP-116
OVERALL TOXICITY AND SAFETY CONCERNS OF

NANOTECHNOLOGY AND ITS REGULATORY NEEDS
Umang Ahlawat1*, Rekha1, Anu Naruka2, Priti Saini3 and Leela Wati1
Dept. of Microbiology, College of Basic Sciences and Humanities, 2 Dept. of Genetics and plant
breeding, College of Agriculture, 3Dept. of MBB&B, College of Basic Sciences and Humanities,
CCS Haryana Agricultural Universiity
Email: umang.ahlawat04@gmail.com
Nanotechnology is seen as a transformative technology, which has the potential to

stimulate scientific innovation while greatly benefiting society. However, the enthusiasm
with which the scientific and technical communities are embracing the technology is
being tempered by concerns over possible downsides, including risks to human health.
Real and perceived adverse consequences in areas such as asbestos, nuclear power, and
genetically modified organisms have engendered increasing skepticism over the ability
of scientists, industry, and governments to ensure the safety of new technologies. As
nanotechnology moves toward widespread commercialization, it is also expanding
beyond traditional knowledge-based risk management to incorporate public perception,
trust, and acceptance. The recent emergence of nanotechnology has ushered humans into
an era of exposure to intentionally created nanosized particles. Once in the blood stream,
nanoparticles can attach to red blood cell causing an increased susceptibility to clotting.
But exposure levels influence toxicity in humans, and safe handling of these materials
can reduce exposure to allowable levels. At the moment, a number of steps have been
taken by various federal agencies to begin the process of generating concrete regulations
and standards. The regulations of nanotechnology must focus more on preventing
deliberate destructive uses of nanotechnology rather than preventing accidents. This is
likely to involve several complementary approaches like access limitation, export
controls, professional ethics and inherent safety. Furthermore, an open source approach
to nanotechnology architectures might be helpful in producing systems that are robust
and resistant to abuse.
SP-117
NANOTECHNOLOGY: A TOOL FOR ELIMINATING

ABIOTIC STRESSES IN PLANTS
Vinita Rajput1, Sonia Rani2 and Ravika3
Department of Horticulture, Department of Soil Science, Department of Genetics and Plant Breeding
College of Agriculture, CCS Haryana Agricultural University, Hisar, Haryana-125004
Email: rockingeverytime@gmail.com
Abiotic stresses are the most significant causes of yield losses in plants, implicated to
reduce yields by as much as 50%. The major abiotic stresses include drought, salinity,
temperature etc. As traditional approaches for crop improvement reach their limits,
agriculture has to adopt novel approaches to meet the demands of an ever growing world
population. Nanotechnology can be the potential answer to this search of novelty, being an
enthralling scientific field, which offers multidirectional research in the area of agriculture
as well as biotechnology. Nanoparticles can be synthesized from metal or metal oxide
through various approaches i.e physical, chemical and biological. With emergence of
nanotechnology and its wide spectrum applications in diverse fields, more attention is
being paid on the synthesis of nanomaterial from metals (Au, Ag, Pd etc) or metal oxides
(ZnO, SiO2, TiO2, etc). Such particles can mediate several important key effects in higher
plants leading to enhance abiotic stress tolerance i.e., enhancement of antioxidant enzyme
activation, enhanced uptake process, co-precipitation of toxic metal ions, immobilization
of toxic metal ions in growth media and compartmentation of metal ions within plants. All
such processes increase plant capabilities to withstand abiotic stresses i.e., salinity,
drought, heavy metal toxicity etc. It is essential to understand plant nanoparticles
interaction and optimization of size and concentration of NPs before practical applications
in the fields so that their possible negative impact can be reduced on natural environment
and crops as well.
SP-118
NANOSENSORS FOR DETECTION AND

QUANTIFICATION OF miRNA
Vrantika Chaudhary and Preeti Saini
Email: vrantika@gmail.com
MicroRNAs (miRNA) are short non-coding RNA molecules that have important role in
developmental, metabolic and disease processes of plants and animals. MiRNA profiles
are specific for cell lineages and tissues, and their changes reflect pathological processes.
Therefore, accurate and sensitive quantification of miRNAs may result in correct disease
diagnosis establishing these small non-coding RNA transcripts as valuable biomarkers.
The innovative new tools for rapid specific and sensitive detection of miRNAs is
important field of research which helps in overcoming some limitations of conventional
quantification strategies, nanotechnology is currently providing significant alternative to
miRNA sensing. Nanoparticles have outstanding optical properties that are valuable for
the development of sensing strategies including gold nanoparticles (AuNPs) and
carbonaceous nanoparticles, such as carbon nanoparticles (CNPs) and nanosized
graphene oxide,which possess outstanding fluorescence quenching efficienct that allows
their implementation in fluorescence recovery strategies. Silver nanoclusters (AgNCs)
and quantum dots (QDs) are instead very bright and stable fluorescence emitters and can
be used either as such or in fluorescence resonance energy transfer (FRET) strategies.
Using the fluorescence properties of DNA-nanosilver clusters that can detect the
presence of target miRNA, a very sensitive method for miRNA analysis using hairpin
probes, T4 ligase, and the fluorescent detection of Cd2+ ions is developed in which target
miRNAs bind to carefully designed stem-loop probes which are then subsequently
hybridized with complementary CdSe nanoparticle-labeled DNA. The wide spectrum of
nanotechnology-based strategies for miRNA quantification that have appeared in the
very recent scientific literature is a sign of a flourishing research area with still plenty of
opportunities to develop sensitive, reliable, robust, and cost effective miRNA sensors.
SP-119
ENZYME IMMOBILIZATION: NANOPARTICLES AS

IMMOBILIZATION MATRIX
Harnek Singh Saini1, Ritu Saini2, Divya Bhatia1 and Naveen Bedi1
1
2
Department of Biotechnology Engineering, UIET, Kurukshetra University, Kurukshetra-136119

Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar-125004
Email: harneksingh89@gmail.com
Immobilization process is to optimize the operational performance of an enzyme for

industrial applications. The current demand is to develop and implement new technologies
in order to enhance enzyme immobilization. With the advent of nanotechnology, the
nanomaterials because of their unique physico-chemical properties constitute novel and
interesting matrices for enzyme immobilization. Immobilization process has been used for
enhancing enzyme activity and stability in aqueous and non-aqueous media. The
entrapment of enzymes in suitable matrices facilitates enhanced catalytic activity, stability,
catalyst recovery, loading ability and reusability etc. Although, the use of chemical catalyst
has been replaced by enzymes for industrial purposes, commercialization of immobilized
enzymes is still a limiting factor. For industrial point of view, reusability and operational
stability are key factors for its efficient application. Nanoparticles strongly influence the
mechanical properties of the material like stiffness and elasticity and also provide
biocompatible environments for enzyme immobilization. Nanoparticles offers highly
efficient and cost-effective enzyme carrier with promising applications in various
industries. Enzymes immobilized onto surface of nanoparticles, offers enhanced storage
stability, thermostability and reusability which promises its potential application in food
and detergent industries.
SP-120
NANOTECHNOLOGY IN BIOPESTICIDES AGAINST

PLANT PARASITIC NEMATODES
Neeraj, Gurpreet Singh and Vinod
Department of Nematology, CCS Haryana Agricultural University, Hisar-125 004, India
E-mail: neeraj17051992@gmail.com
Biopesticides are the formulated form of active ingredients originating from bacteria,
viruses, fungi and plants extracts. The word Nano is developed from the Greek word
meaning dwarf. In more technical terms, the word nano means 10-9, or one billionth
of something. Nanoparticles display slow release of encapsulated functional molecules and
reduce its frequent applications. Nanoparticles are smaller in size with more charge and
larger surface area with higher stability and solubility, so behave differently from their
bulk sized counterparts For example, a virus is roughly 100 nm in size. The potential uses
and benefits of nanotechnology are enormous. Thus, nanotechnology has become one of
the most promising new technologies in the recent decade. Pests, including insects, mites,
nematodes and pathogens, are the major limiting factor in profitable crop production.
Frequent application of pesticides has resulted in development of pest and disease
resistance, accumulating residues in produce and environmental pollution. So there is a
need for alternative approach as to control pests and pathogens. With the goal to promote
its use for agricultural applications, nano materials (NM) can be effectively used in plant
germination and growth. Moraxella osloensis associated with Phasmarhabditis
hermaphrodita produces an endotoxin which is heat and protease tolerant and biologically
control mollusk pests (slug parasitic nematode). Silver nanoparticles (AgNP) improved
turfgrass quality in one year and reduced gall formation in the roots in two years without
phytotoxicity. Silicon (Si) increases disease resistance and stress resistance in plants). It
also stimulates the physiological activity and growth of plants.
SP-121
NANOMATERIAL BASED ELECTROCHEMICAL

BIOSENSORS FOR FOOD BORNE PATHOGENS
Simran Arora, Saleem Siddiqui and Rakesh Gehlot
Centre of Food Science and Technology
CCS Haryana Agricultural University, Hisar-125 004, India
Email: arorasimran245@gmail.com
Food safety is increasingly becoming an important public health issue as foodborne

diseases present a widespread and growing public health problem in both developed and
developing countries. The rapid and precise monitoring and detection of foodborne
pathogens are some of the most effective ways to control and prevent human foodborne
infections. Conventional methods for bacterial pathogens detection are time consuming
and laborious, requiring certain sophisticated instruments. Biosensors offer rapid and cost
effective method for detection of foodborne pathogen. It utilizes the unique properties of
biological and physical materials to recognize a target molecule. In recent years,
nanotechnology has emerged as a promising field for solving food safety issues in terms of
detecting contaminants. The sensing capacity of the detection systems is being improved
by using nanomaterials such as magnetic nanoparticles (MNPs), carbon nanotubes (CNTs),
nanorods (NRs), quantum dots (QDs), nanowires (NWs), nanochannels (NCs), etc. The
introduction of nanomaterials into electrochemical sensors makes them suitable to reach
lower detection limit, higher sensitivity values and bring novel labelling opportunities
including multidetection capabilities. Nanomaterials can contribute as labels or transducers
modifiers so as to improve the performance of the biosensor.
SP-122
NANOTECHNOLOGY FOR FOOD PRESERVATION

Mohammad Sadiq, Anju Kumari, Rakesh Gehlot and Kanika Panwar
Centre of Food Science and Technology, CCS Haryana Agricultural University, Hisar-125 004, Haryana,
India
E mail:sadiq.lodin786@gmail.com
The pre and postharvest issues related to agricultural produce have been remarkably
reduced due to the application of nanotechnology for the preservation of the food products.
The preservation industry has been by almost 1012% per year. Food packaging methods
are used to make sure that the quality of the food is kept intact however; they are packaged
in a way so that it is safe for consumption. Production, processing, and shipment of food
products could be made more secure through the use of nanosensors for pathogen and
contaminant detection. Silver, a well-known antimicrobial agent, is being infused into
storage containers to retard bacterial growth and allow for longer storage of foods. In a
case study, the 24-hour growth of bacteria was reduced by over 98 percent because of the
silver nanoparticles. Nanomaterials are being developed with enhanced mechanical and
thermal properties to ensure better protection of foods from exterior mechanical, thermal,
chemical, or microbiological effects. Nanocomposites, for instance, are nanoparticles
bonded in polymers so that the materials have enhanced properties such a lighter weight
and better recyclability, as well as spoilage and flavor issues. Nanocomposite materials are
being used in beer bottles; allowing for a 6-month shelf life. So, with nanotechnology, the
shelf life of foods can be increased and the extent of food spoilage can be decreased.
SP-123
MAGNETIC NANOPARTICLES: PHYSICAL AND

CHEMICAL CHARACTERISTICS AND THEIR
APPLICATION
Reeti Panchal, Sudesh Rani
Department of Zoology, M.D.U. Rohtak
Email: reetipanchal@gmail.com
Nanoparticles (NPs) of diameters ranging from 1 to 100 nm are submicron particles and
consist of inorganic or organic materials. One of the types of NPs is magnetic
nanoparticles (MNPs) which produce some response to applying magnetic field. The
crucial characteristics of MNPs are high magnetic susceptibility, non-toxicity,
superparamgnetic, high coercivity, low Curie temperature and biocompatibility. The two
important advantages of MNPs are their lower sedimentation rates and high effective
surface areas. The MPNs are used in the removal of high concentration of organic
compounds. The biomedical uses of MNPs were reviewed in different parts: cancer
therapy, the delivery of drugs and genes, magnetic resonance imaging (MRI), tissue
engineering, bone and dental repair and in biosensors. This review shows the important
physical and chemical properties of MNPs and their applications. Although lots of work
has been done, but still much more required. New methods and techniques need to be
developing the MNPs with satisfactory biocompatibility. Finally, more understanding and
further study of the properties of MNPs are needed to reveal their new applications.
SP-124
SYNTHESIS AND CHARACTERIZATION OF ZINC

OXIDE NANOPARTICLES AND THEIR APPLICATION
IN THE TEXTILES AS UV-ABSORBERS
Pooja and Anil Dhaka
Department of Botany, Pt.N.R.S Govt. College, Rohtak (Haryana)-124001
Email: pj7790@gmail.com
In the textile industry the use of nanotechnology has increased rapidly. This is mainly
because the conventional methods which are used to impart different properties to fabrics
often do not lead to permanent effects and will lose their functions after laundering or
wearing. But Nanoparticles can provide high durability for treated fabrics with respect
to conventional materials because they possess large surface area and high surface
energy that ensure better affinity for fabrics and lead to an increase in durability of the
textile functions. The coating of Nanoparticle affect the fabric properties like dyeing
capacity, tensile strength, bursting strength, bending rigidity, air permeability (comfort)
and fabric friction that play a crucial role in textile industries. But Zinc oxide is widely
used in different areas because of its unique photocatalytic, electrical, electronic, optical,
dermatological, and antibacterial properties. Zinc oxide is actually one of the best
absorbers of UV radiation that mainly come on Earth from the Sun through the
atmospheric ozone layer. Synthesis and characterization of ZnO nanoparticles obtained
through a homogeneous phase reaction between zinc chloride and sodium hydroxide at
high temperature.
COMPARATIVE PROTEOMIC ANALYSIS OF

POSTHARVEST STORED TOMATO (SOLANUM
LYCOPERSICUML.) FRUITS TREATED WITH SALICYLIC ACID A
CONJUGATE AND CARRIER FOR NANOPARTICLE
SP-125
Naresh Kumar*, Jayanti Tokas and H.R.Singal

Dept. of Chemistry and Biochemistry, College of Basic Sciences and Humanities, CCSHAU, Hisar, India
Email: Nareshkumarbiotech85@Gmail.Com
The novel use of nanoparticles tagged to agrochemicals can reduce the damage to other
plant tissues and can be applied to prolong the postharvest storage ability in climacteric
fruits. The strategy was to explore the comparative proteomic analysis of nanoparticle
carrier salicylic acid treated postharvest tomato fruits and untreated fruits during ripening.
Treated fruits were stored at room temperature and samples were taken for proteomic
analysis at 3 day interval till complete decay of tomato fruits. Hundreds of protein
components as spots were resolved on 2-DE gels (pH range 3-10) stained with silver
nitrate and almost 60% presented overlapping gel coordinates between the treated and
untreated tomato fruits. The spots on gels of different groups were analyzed by
densitometric software (PD Quest, Bio-rad) to identify the differentially expressed proteins
as compared to control. In general, common variably expressed spots in both group during
ripening can be associated to important physiological processes such as redox status
control, defense, stress, carbon metabolism, energy production, cellular signaling and
ethylene production metabolism. Out of ~280 protein spots identified in each gel, ~20-25
spots were differentially expressed during ripening of fruits.
EFFECT OF NANOCHITOSAN COATING ON

POSTHARVEST QUALITY AND CELL WALL
DEGRADING ENZYMES OF GUAVA (PSIDIUM GUAJAVA) FRUITS
DURING STORAGE
SP-126
Shilpa Chawla and Veena Jain

Department of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar-125004, Haryana
Email: sunsshine2009@gmail.com
Current investigations were carried out to assess the effectiveness of chitosan coating with
calcium chloride (CaCl2) on firmness, cell wall components and its degrading enzymes of
guava fruit variety Hisar Surkha. Fruits were harvested at mature green stage and were
given postharvest treatment of 1.5% chitosan, 1.5% CaCl2, 1.5% chitosan in combination
with 1.5% CaCl2 and water (control) for five minutes followed by storage at room
temperature. Samples were analyzed at three day interval until complete decay. The results
demonstrated that chitosan treatment could keep the freshness of fruit and reduce weight
loss of samples. Combination treatment of chitosan (1.5%) with CaCl2 (1.5%) provided an
effective control in maintaining firmness, retention of cell wall components viz. cellulose,
hemicelulose and pectin. The activities of cell wall degrading enzymes, pectin
methylesterase (PE), polygalacturonase (PG) and cellulase were also assayed. PG and
cellulose exhibited progressive increase in activity during storage but increase was much
less in 1.5% chitosan in combination with 1.5% CaCl2 treated fruits followed by 1.5%
chitosan alone and 1.5% CaCl2 alone. PE activity increased upto 3th day and then
declined. Similarly, 1.5% chitosan +1.5% CaCl2 treated fruits exhibited slow increase in
PE activity. However, functions created by using chitosan are restricted because of high
concentration needed for such properties. Chitosan functionalities could be enhanced by
using its nano-structure to prolong guava shelf-life for a month as chitosan nano-particle
has high efficiency in extending the shelf-life of fruits.
SP-127
ENVIROFRIENDLY METHOD FOR BIOGENIC

SILVER NANOPARTICLES PRODUCTION
Hirdayesh Anuragi, Bharat Taindu Jain
Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, Haryana.
Email: heartyanuragi@gmail.com
Nanoparticles beingfundamental building blocks of nanotechnology,put forward an

essentially useful platform, demonstrating unique properties with potentially wide-ranging
biological applications.Conventionally silver nanoparticles are synthesized by chemical
method using chemicals as reducing agents which later on become accountable for various
biological risks due to their general toxicity; engendering the serious concern to develop
environment friendly processes. Use of agricultural crop plant extracts for biosynthesis of
nanoparticles has drawn more interest of researchers becauseit is rapid, cost-effective and
eco-friendlyas comparable to those of chemical and physical methods, providinga new
dimension to the agricultural sector in the utilization of crop waste. The silver
nanoparticles can be prepared by treatingaqueous solution of AgNO3 solution with leaf
extract of common crops like Psidiumguajava, Vigna radiata, Arachishypogaea,
Cyamopsistetragonolobus, Zea mays, Pennisetumglaucum, Sorghum vulgareetc.
Reduction of Ag+ ion into Ag nanoparticles using leaf extract is evidenced by visual
change incolour of reaction mixture and UVVisible spectroscopy is utilized to monitor
the formation of silver nanoparticles.The solution is kept at 4 C for future use.These Ag
nanoparticles prepared byleaf extracts are more stable without aggregation.Biosynthesis
of such nanoparticles is single a step process,environment friendly and
commerciallyeconomic, providing enormousand potential scope for future developments
in the area of electrochemical sensor, biosensors, medicine, healthcare and agriculture.
SP-128
TOXICITY OF CdCl2 ON SEEDLING GROWTH OF

MUNGBEAN GENOTYPES AND FUTURE PROSPECTS OF
AMELIORATION THROUGH NANOTECHNOLOGICAL
INTERVENTION
Sunder S. Arya1 and Sarita Devi2
1
Department of Botany, Maharshi Dayanand University, Rohtak

2
Email: aryasunder.hau@gmail.com
The influence of cadmium was studied on growth and development of seedlings in

mungbean genotypes (i.e. Basanti, Satya and MH 421). Results revealed that fresh (FW)
and dry weight (DW) (mg) of shoot were significantly decreased from 297.9 to 237.3 and
28.9 to 17.4, respectively. The FW and DW of root also showed same trend, the values
varied from 17.2 to 9.1 and 2.7 to 1.2, respectively upon CdCl2 application. Decline in
RWC (%) was noticed. The ratio of chl a : chl b and chlorophyll : carotenoid also showed
declining trend i.e. from 2.67 to 2.48 and 2.78 to 2.37, respectively. Whereas, increase in
lipid peroxidation (MDA content) and proline content was observed and magnitude of
increase varied from 0.93 to 1.53 mM MDA g-1 FW and 7.57 to 16.36 mM g-1 FW on
imposition of CdCl2. However, total soluble carbohydrate and protein content showed
reverse trend and maximum percent decline was observed in Basanti (15.32 and 19.61 %)
and minimum in MH 421 (2.23 and 1.15 %) genotype. The genotype MH 421 showed
relatively better tolerance to cadmium chloride stress than other genotypes and can be a
potential alternative for cadmium-contaminated soils. Nanotechnology is fast growing
technology so keeping in view of our results, the use of nanoparticles in sustainable
agriculture and crop improvement are still at juvenile phase. Therefore it would be useful
to understand plantnanoparticles interaction.
SP-129
INFLUENCE OF NANOPARTICLE ON SYMBIOTIC

BACTERIUM RHIZOBIAL STRAIN
Subha Dhull, Rajesh Gera, Aarti Yadav, Swati
Department of Microbiology, CCS Haryana Agricultural University,
Hisar-125 004
Email: subhachoudhary45@gmail.com
Nanotechnology is the study and design of machines on the molecular and atomic level. In
regard to medicine and therapeutics, this field is emerging as a new scope for the treatment
of various diseases. In addition, nanotechnology is also likely to influence agricultural
research especially in the conversion of agricultural and food wastes to energy and other
useful by-products through enzymatic nano-bio-processing, disease prevention and
treatment of plants using various nanomaterials. Metallic nanoparticles are found to be
most promising as they possess remarkable antimicrobial properties. A number of
approaches are available for the production of metal nanoparticles such as chemical
reduction, electrochemical techniques and green chemistry route. It is claimed that
nanoparticles synthesized using biological route is environmentally safer and also
biocompatible. Recently, nanoparticles have been reported to cause biologically
undesirable toxic effects on both deleterious and beneficial rhizosphere microorganisms
including Escherichia coli, Bacillus subtilis, and Rhizobium. In the present investigation,
209 rhizobial strain were isolated from arid and hyper-arid zones of Rajasthan state. All
rhizobial strain were screened for nodulation efficiency under sterilization condition. Out
of these 3 rhizobial strain were able to effectively nodulate six legumes viz., clusterbean,
mungbean, mothbean, cowpea, pigeonpea and urdbean, four rhizobial strain nodulated 5
different legume crops. The effect of different nanoparticles could be checked on the plant
growth promoting activity like siderophore and IAA production of these seven
promiscuous rhizobial strains.
SP-130
EFFECT OF TDZ ON SHOOT ORGANOGENESIS

AND SOMATIC EMBRYOGENESIS IN VIGNA RADIATA (L.
WILCZEK) AND ITS APPLICATION POTENTIAL FOR
NANOPARTICLES-MEDIATED GENE TRANSFER
Manish Sainger1, Darshna Chaudhary2, Kiran Yadav2, Sonia Kapoor1, Ranjana Jaiwal3, and
Pawan K. Jaiwal2
1
Deptt. of Biotechnology, UIET, M.D. University, Rohtak 124001, India

2
Centre for Biotechnology, M.D. University, Rohtak 124001, India
3
Deptt. of Zoology, M.D. University, Rohtak 124001, India
Email: Msainger@Gmail.Com
Mungbean also known as green gram is a warm season legume of family leguminoseae.
Green gram is one of the most important pulse crops, grown throughout the world
primarily for its protein rich edible seeds and sprouts. However, the productivity is
severely reduced because of distinctive abiotic and biotic stresses. Genetic improvement of
mungbean through conventional breeding has been slow due to the lack of desirable and
satisfactory level of variability in germplasm. The only option left is to transfer genes from
diverse sources and an in vitro regeneration protocol is a prerequisite for genetic
transformation methods. The present study was undertaken to analyse the effect of
different concentrations of TDZ on shoot regeneration and somatic embryogenesis from
the cotyledonary node and whole seed explants in Vigna radiata. TDZ at concentrations
lower than 5.0M induced shoot organogenesis whereas at higher concentration (10.0M),
it caused a shift in regeneration from organogenesis to somatic embryogenesis. The study
shows that the formation of embryogenic tissues from somatic cells can be stimulated by
applying TDZ and TDZ at low concentrations induced shoot organogenesis whereas at
high concentrations stimulated in vitro embryogenesis. This in vitro regeneration protocol
can be further used for genetic transformation in plants and have a great potential to be
used in nanoparticles-mediated gene transfer methods. The nanoparticles, which can be
used as a vector for gene transfer, include calcium phosphate, carbon based, silica, gold,
magnetite, strontium phosphate, magnesium phosphate, and manganese phosphate. Small
size of the nanoparticles allows them to bypass the cell barriers and directly transfer DNA
into the cells achieving stable integration and rapid expression of the transgene.
TDZ INDUCED SHOOT ORGANOGENESIS AND

SOMATIC EMBRYOGENESIS IN VIGNA UNGUICULATA
(L.WALP) AND ITS FEASIBILITY FOR NANO-BASED GENETIC
TRANSFORMATION
SP-131
Darshna Chaudhary1, Rachna Dhankher1,Manish Sainger2, Ranjana Jaiwal3 and Pawan K. Jaiwal2
1
Centre for Biotechnology, M.D. University, Rohtak 124001, India

Deptt. of Biotechnology, UIET, M.D. University, Rohtak 124001, India
3
Deptt. of Zoology, M.D. University, Rohtak 124001, India
Email: Darshnachaudhary@Yahoo.Com
Cowpea belongs to family leguminoseae and is a large seeded legume grown for its protein
rich grain by resource poor farmers of under developed and developing countries. The
productiveness and best of the cowpea grain is severely reduced because of distinctive
abiotic and biotic stresses. Genetic improvement of cowpea through conventional breeding
has been slow due to the lack of desirable and satisfactory level of variability in
germplasm. The only option left is to transfer genes from diverse sources and an in vitro
regeneration protocol is a prerequisite for genetic transformation methods. The present
study was undertaken to analyse the effect of different concentrations of TDZ on shoot
regeneration and somatic embryogenesis from the cotyledonary node and whole seed
explants in cowpea. TDZ at 1.0M was found to induce shoot buds in maximum number
of cultures (98%). This response of TDZ decreased with increase in the concentration from
1.0 -5.0M. TDZ at 10.0M induced only somatic embryos (30) in 10% of the cultures.
An average of 30 somatic embryos observed in the nodal region of seedlings after 20-25
days of culture. TDZ at concentrations lower than 5.0M induced shoot organogenesis
whereas at higher concentration (10.0M), it caused a shift in regeneration from
organogenesis to somatic embryogenesis. This in vitro regeneration protocol can be further
used for genetic transformation in plants and have a great potential to be used in
nanoparticles-mediated gene transfer methods which are new and have the potential to
directly transfer DNA into the cells, achieving stable integration and rapid expression of
the transgene. Small size of the nanoparticles allows them to bypass the cell barriers like
cell wall and plasma membrane and deliver genes into the cell of living systems.
SP-132
MANAGEMENT OF AGRI-BYPRODUCTS
THROUGH THEIR VALUE ADDITION INTO
POLYHYDROXYBUTYRATE (PHB)
Virendra K Sikka1 and Mukesh Rani2
1 Department of Molecular Biology, Biotechnology & Bioinformatics, CCS HAU, Hisar

2 Department of Biotechnology, Government PG College, Hisar
Email: 1vksikka@Gmail.Com
PHBs produced by bacterial action, is a rare example of hydrophobic polymer that is truly
biocompatible and biodegradable with high melting temperature and crystallinity, clay
nanocomposites. Research efforts at MBB, CCS HAU have lead to the development of
farmer friendly rhizobia capable of efficiently converting agri byproducts (biowaste) into
valuable PHB in an ecofriendly biological process. The AgroPHB as blends into bioplastic
can serve as a tangible alternative to zenobiotic and recalcitrant polyethylene to alleviate
its sewage and animal alimentary canal choking hazards. The AgroPHB from rhizobia has
been found to be blendable with polyethylene and on blending will provide alternative to
polybags. The PHB portion shall get decomposed rendering disintegration of the
polyethylene films thus minimizing the damages caused.
BIODEGRADABLE POLYHYDROXYALKANOATESPHBs THE ECOFRIENDLY NANOPARTICLES
SP-133
Virendra K Sikka1 and Mukesh Rani2

2
Department of Biotechnology, Government PG College, Hisar
Email: 1vksikka@Gmail.Com
Polyhydroxybutyrate (PHBs) have been widely hailed as a "green" alternatives to

petroleum-based plastic for use in packaging, agricultural and biomedical applications
PHB, produced by bacterial action, is a rare example of hydrophobic polymer that is truly
biocompatible and biodegradable with high melting temperature and crystallinity. The clay
nanocomposites are nanohybrids like neat polymer and the natural biodegradation of PHB
nanocomposites and could lead to wider use of PHB plastics. The rate of biodegradation
could be fine-tuned by adjusting the amount of admixture additives. Research efforts at
MBB, CCS HAU have lead to development of microbes capable of efficiently converting
agri byproducts (biowaste) into valuable PHB in an ecofriendly biological process. The
AgroPHB as blends into bioplastic can serve as a tangible alternative to zenobiotic and
recalcitrant polyethylene to alleviate its sewage and animal alimentary canal choking
hazards. The films of bioplastic could find effective use as coatings for slow-release
fertilisers nutrients and even water through hydogels for plants. Nanocapsules for
pesticides and herbicide application with efficient dosages. These efforts can lead us to
agricultural productivity enhancement.
IMPROVEMENT OF BIO-FERTILIZER ACTIVITY

USING BIO-NANO TOOLS
SP-134
Ikbal1*, Minakshi P2, Basanti Brar2, Koushlesh Ranjan2 and V. K. Sikka1

1
Department of Biotechnology and Molecular Biology, CCS HAU, Hisar, Haryana, India
2
Department of Animal Biotechnology, LUVAS, Hisar, Haryana, India
Email: iqbalshah5330@gmail.com
Environmental issues such as freshwater pollution, energy saving, and insufficient

nutrients in soil are forcing the farmers to introduce methods of cultivation that have a
lower impact on the environment. Biological nitrogen fixation (BNF) can offer this
alternative in farming practices that changes inert atmospheric N2 into the plant usable
ammonia (NH3). Rhizobium are well known group of bacteria that acts as the primary
symbiotic fixer of nitrogen and used as biofertilizer. This is an important alternative
technology to control environmental pollution, since the negative externalities of chemical
fertilizers have become well known. For the past few years, the agricultural practices have
changed from chemical to organic farming (environment friendly) and the choice of bionanotechnologies associated with Rhizobium biofertilizer production is a key to successful
application and also provide new avenues for the development of agriculture system. The
physical stability and the high surface area of nanotubes, together with the ease and costeffective fabrication of nanotube membranes may thus expand their use in the production
of biofertilizer. We developed promiscous rhizobia which are capable to infect across strict
host barriers effectively. For this native rhizobia were collected from different virgin
locations of Haryana. These isolates were mutated through gamma irradiation. Modified
strains of Rhizobium were evaluated for their ability to form effective symbioses with
Chickpea plants under field conditions. The application of this promiscuous Rhizobium as
biofertilizer was successfully used up to one month on storage at 4C. A significant
enhancement in plant growth, nitrogen fixation and superior symbiotic properties were
observed in field. Rhizobium biofertilizer increase the nodule formation in chickpea up to
48% and plant weight up to 25%. In futuristic approach the integration of Rhizobium with
nanostructures may leads to hybrid nano-biofertilizer that is effective after 12 weeks (3
months) of storage at room temperature. The same technology could therefore be applied
to other bacterial cultures and deliver them to the plant. The nano-biofertilizer are cost
effective, eco-friendly which increased shelf life and easy to store at room temperature.
SP-351
USE OF ZINC OXIDE NANOPARTICLES FOR CROP

IMPROVEMENT
Vineeta Pandey and O.S. Dahiya
Department of Seed Science and Technology,CCS, HAU, Hisar,Haryana-125004
Nanoparticle provides an efficient means to distribute pesticides and fertilizers in a

controlled fashion with high site specificity thus reducing collateral damage. Among
different nanoparticles zinc oxide nanoparticles (ZnO NPs) have remarkable optical,
physical, and antimicrobial properties and therefore have great potential to enhance
agriculture. Zinc oxide NPs have potential to boost the yield and growth of food crops. The
colloidal solution of zinc oxide nanoparticles is used as nanofertilizer. Nanofertilizer is a
plant nutrient which is more than a fertilizer because it not only supplies nutrients for the
plant but also revives the soil to an organic state without the harmful factors of chemical
fertilizer. Zinc oxide nanoscale treatment (25 nm mean particle size) at 1000 ppm
concentration, promoted seed germination, seedling vigour, and plant growth in different
crops. Zinc oxide nanoparticles (ZnO NPs) have unique physical and chemical
characteristics which deviate from larger particles of the same material, due to their
extremely small size, higher specific surface area and surface reactivity. The peculiar
properties of ZnO NPs could potentially improve zinc (Zn) fertilizers for sustainable
agriculture. This is based on the assumption that ZnO NPs provide a more soluble and bio
available source of Zn in soil compared to micron-or millimetre-sized (bulk) ZnO particles
currently used for Zn fertilizers in Zn deficient soils. If such nano-scale materials were
applied to supplying plant Zn requirements, their effect on nutrient accessibility by plant
roots could be significantly enhanced resulting in increased yields and Zn content of crops.
SP-136
ELECTROSPUN NANOFIBERS FOR

BIODEGRADABLE FOOD PACKAGING MATERIALS
Sucheta, Rakesh Gehlot, Saleem Siddiqui and Simran Arora
Centre of Food Science and Technology, CCS Haryana Agricultural University, Hisar
Email: Suchetakkr @gmail.com
Food industry uses large quantity of plastics for food packaging and discarded plastic
waste creates threat to environment. Biodegradable films from food and agriculture waste
are in limited use because of poor barrier properties. The strategies to eradicate this
problem are blending of polymers, coating with high barrier materials and use of
multilayered films containing high barrier film. Electrospinning is a novel fabrication
technology based on high electric fields that can be used to produce polymer and
biopolymer based mats composed of nanofibers or other nanostructures. In this, solid
polymer strands are produced from solution by applying a strong electric field to a
spinneret with a small capillary orifice. Nanofibers can enhance the physical and barrier
properties of plastics and bioplastics and can deliver nutraceuticals or antimicrobials to
foods. Electrospun nanofibres have been used for developing smart packaging capable of
responding to external stimuli such as temperature changes. Recent advances include
development of packaging mats using biodegradable polymers with natural antimicrobials
such as olive oil and shows functional effect at food surfaces where microbial growth is
localized rather than mixing antimicrobial with food. Application of electrospun
nanofibers for food and agricultural systems are relatively few because of use of synthetic
polymers, but progress in the production of nanofibers from food biopolymers will likely
increase the use of biopolymeric nanofibers in the food industry.
SP-137
VALUE ADDITION WITH NANOTECHNOLOGY IN

HORTICULTURE AND VEGETABLE CROPS
Sonia Rani, P.S. Shehrawat and Kanika Rani
Department of Extension Education,
CCS Haryana Agricultural University, Hisar-125001, India
Adding value is the process of changing or transforming a product from its original state to
a more valuable state. Value added products made by fruit and vegetables are candy, jam,
jelly, chwnayprash, pickles, squash, juice, chips, marmland, processed vegetables, canned
vegetables, frozen vegetables, French fries, potato chips, dried leafy vegetables (methi,
palak, kadhi patta), dry chilli. Indias one-third of fruits and vegetables produce rots
because of poor storage technologies and infrastructure, transport and distribution system.
On the other hand, unsold fruits and vegetables is a huge loss, as these products are highly
perishable and cannot be stored for long time without cold storage facilities or high tech
warehouses. The global efforts to achieve sustainable growth and access in the supply of
safe and healthy food are services based on food processing technology. Therefore, valueaddition with nanotechnology may raise the value of products or something can add that
enables to increase the profit margin and demand for consumption. Its confirm that
incremental application of nanotechnology in the horticultural, first in the fruit packaging
and later in other areas such as tracking, tracing, storage and distribution, is occurring.
Raising awareness of nanotechnology in the agri-food sector, including feed and food
ingredients, intelligent packaging and quick-detection systems, is one of the keys to
influencing consumer acceptance. Nanotechnology use may bring potential benefits to
farmers through food production and to the food industry through development of
innovative products through food processing, preservation, and packaging.

SP-138
PHYTOSYNTHESIS OF NANOPARTICLES: WHICH

ONE BETTER, PLANTS OR PLANT EXTRACTS
Nikhil Mehta, Priyanka Rao and Raman Saini
Department of Biotechnology, Kurukshetra University, Kurukshetra-136 119
Email: ramanksaini@rediffmail.com
Nanoparticles have been a subject of interest in these days due to their wide applications in
various fields such as material science, biotechnology and organic chemistry for their
functions as molecular markers, diagnostic imaging and catalysis. Most of these particles
are manufactured chemically using many step processes that require various stabilizers for
their stabilization. This generally results in the increase of cost and low yield. So there was
a need for the alternatives for manufacturing of the same. For this, various plants and their
extracts have been used in manufacturing of nanoparticles in less time and relatively
simple method, but approaches differ for the two. Plant extracts can be used for production
of nanoparticles in tube at lab scale. The method is quite simple and homogenous solution
can be used for the preparation of nanoparticles. The extraction of the nanoparticles from
the extract requires simple procedure for separation. but the properties of the particles thus
obtained varies from batch to batch and also the variations have been obtained by
modifying the conditions of manufacturing like pH and temperature, which in many cases
is not desirable. So the problem can me more or less be solved by using the intact plant
system for the manufacturing of particles rather than using plant extracts. The constituents
that are responsible for the manufacturing of nanoparticles of metals from their compound
form vary from batch to batch in extracts whereas intact plants can maintain continuous
supply. Moreover plants provide large scale setup for the manufacturing of such particles
in a green and cleaner way without using any stabilizers and also can be stored in the
plants itself for long time. So the plants can be seen as brighter biofactories for the
manufacturing of metallic nanoparticles in a less time and more eco friendly way.
SP-139
NANOTECHNOLOGY: INDUSTRIAL APPLICATIONS

AND FUTURE PROSPECTS
Naveen Bedia, Divya Bhatiaa, Harnek Singh Sainia and Ritu Sainib
a
Department of Biotechnology, UIET, Kurukshetra University, Kurukshetra-136119

Department of Chemistry and Biochemistry, CCS Haryana Agricultural University
Email: naveen.bedi90@gmail.com
In recent days, Nanotechnology has gained utmost importance in field of Science and
Technology. The application of nanoscale materials and structures, usually ranging from 1
to 100 nm, is an emerging area of nanoscience and nanotechnology. Nanotechnology has
been embraced by industrial sectors due to its applications in the field of electronic storage
systems, biotechnology, magnetic separation, environmental health, mechanics, optics,
biomedical sciences, chemical industries and nonlinear optical devices and photoelectrochemical. The application of metallic NPs in the medical and biopharmaceutical
fields are both numerous and promising. Nanomaterials often show unique and
considerably changed physical, chemical and biological properties compared to their
macroscaled counterparts. Difference in the physiochemical properties of nanomaterials
can be attributed to their high surface-to-volume ratio. The noble metals, especially silver
and gold, have attracted great attention due to their innumerable applications in various
branches of science, namely catalysis, photonics, photography, chemical sensing, Surface
Enhanced Raman Scattering (SERS), and most importantly, in the medicinal field as antimicrobial agents.
SP-140
A STEP TOWARDS APPLICATION OF LYTIC

BACTERIOPHAGES FOR NANOTECHNOLOGY IN
MEDICINE
Taruna Anand1, B.C. Bera1, Rajesh.K. Vaid1, Nitin Virmani2, Riyesh T1., Kanupriya1,
Medhavi Vashisth1, Sunita Kundu1, B.N. Tripathi1, 2
National Centre for Veterinary Type Cultures, National Research Centre on Equines, Sirsa Road, Hisar
2
National Research Centre on Equines, Sirsa Road, Hisar, Haryana, India
Email: tarunandri@gmail.com
Bacteriophages -the obligate parasites of bacteria. They evolve maintaining a rapidity with
the evolution of their host. Also novel phages are being discovered day by day, thus it is an
important area to explore the phage diversity against various existing and emerging
bacterial strains as well as to study phage biology and host interaction. In the coming era,
where the pathogen resistance will probably increase and physicians will prefer natural
remedies over, synthetic ones, the trend of phage therapy will probably increase. Also the
phages are easy to isolate from environmental samples such as soil, sewage, water bodies
etc. in comparison to tedious processes and intense research involved for drug/vaccine
development. Bacteriophages can be produced in bulk and stored for future use. In the
bacteriophage repository at National Collection of Veterinary Type Cultures (NCVTC) at
Hisar, we have isolated bacteriophages against a variety of pathogens including
Escherichia coli, Serratia marscecenes, Aeromonas hydrophila, Enterobacter spp.,
Bacillus spp., Shigella spp., Klebsiella pneumonia, Salmonella spp., etc. These phages
have been used for large scale preparation of high titre stocks. Pancreatic DNaseI and
RNase were used to degrade any host DNA and bacteriophage particles were precipitated
using PEG8000. Phages were characterized by transmission electron microscopy and
protein profiling by SDS- PAGE. Further phages of myoviridae group were characterized
by PCR amplification of gp23 gene; its sequencing and phylogenetic analysis. As
bacteriophages can be easily engineered genetically, hence they are ideal for nanoparticles
in nanomedicine and in developing nano-probes for disease diagnosis.

SP-141
MANAGEMENT OF RENIFORM NEMATODE,

ROTYLENCHULUS RENIFORMIS ON COWPEA BY USING
BOTANICALS
Saroj Yadav, Jaydeep Patil and M.K. Sharma
Department of Nematology, Rajasthan College of Agriculture,
Maharana Pratap University of Agriculture and Technology, Udaipur-31300, India
Email: sarojhau29@gmail.com
Management of phytoparasitic nematodes with plant products has been supported to

increase awareness of environmental hazards. A screen house experiment was conducted
to control the Reniform Nematode, Rotylenchulus reniformis on cowpea, Vigna
unguiculata L. var. VU-89, a important Kharif pulse crop in India. Different plant
extracts of Ashwaganthan (Withania somnifera), Lantana (Lantana camara) and Aak
(Calotropis gigantea) was used as seed soaking treatment against reniform nematode,
Rotylenchulus reniformis infesting cowpea. Results revealed that the seed treated with 30
per cent concentration of all the plant extracts was found effective while extract of Lantana
(L. camara) leaves to be most effective in comparison to Aak (C. gigantea) leaves and
Ashwaganthan (W. somnifera) leaves extract in improving plant growth on cowpea. L.
camara leaves showed significantly increase in plant growth parameters of shoot length
(35.32 cm), shoot weight (16.47 g), root length (19.45 cm) and root weight (3.15 cm) and
also reduced no. of females per plant (22.0) and larval population in 200 cc soil (296.0) to
a significant level. Seed treatment with lower concentrations of leaf extract also showed
significant control over the untreated inoculated check. The study advocates the
management of nematodes with botanicals which will act as an asset to control
environmental pollutions.
SP-142
NANOTECHNOLOGY: IN SAFETY OF
HORTICULTURAL PRODUCE
Rahul Pippal1, Swetal Rana2 and Payal Rana3
1&2
College of Agriculture, RVSKVV, Gwalior (474020) (M.P.)

3
Krishi College, Jorapali, Raigarh (496001) C.G.
Email: rahulpippal1@gmail.com
Nanotechnology can help in satisfying increased demand from consumers for safe and
quality food and to meet stringent government food safety regulations. Nanotechnology
has shown significant promise in the enhancement of sensors able to detect spoilage or
changes to product quality. To ensure food safety, Good Food Project scientists have
developed a portable nanosensor to detect chemicals, pathogens and toxins in food on real
time basis. Food can be analyzed for safety and quality at control points in the supply
chain; for instance at the farm, abattoir, during shipping, at the warehouse or storage depot,
and at the processing or packaging plant. This circumvents the very time consuming and
expensive alternative of sending samples to laboratories. Now devices are being developed
that uses DNA biochips to detect pathogensa technique that can also be applied to
determine the presence of different kinds of harmful bacteria or fungi affecting fruit. In
addition there are plans to develop microarray sensors that can be used to identify
pesticides in fruit and vegetables. By mean soft he right selection of materials and
packaging technologies, it is possible to keep the product quality and freshness during the
time required for its commercialization and consumption. The nanocomposite materials
obtained by mixing natural polymers and sheets of crystalline solid layered (claysor
LDHs), offer a great variety of property profiles. They are even able to compete, both in
price and in performance, with synthetic polymeric materials in packaging.
Nanotechnology has been widely applied to the food industry. Green tea with nanopacking had better maintenance of vitamin C, chlorophyll, polyphenols and amino acids
than with normal packing.

SP-143
NANOFOOD
Sarita Kumari, Anju Kumari and Sangeeta Sindhu
Department of Food & Nutrition, COHS, CCS Haryana Agricultural University
E mail: vidhuyashvee@gmail.com
Nanotechnology is the basis of many novel and functional foods, and food colors, favors,
and textures can all be manipulated and altered at the nanoscale level. Food is nanofood
when nanoparticles or nanotechnology techniques or tools are used during cultivation,
production, processing, or packaging of the food. It does not mean atomically modified
food or food produced by nanomachines. Nanotechnology may hold the key to solving
many critical issues facing the worlds food supply today. Nanotechnology can provide
manipulation of food polymers and polymeric assemblages to provide tailor-made
improvements to food quality and food safety. Further, foods among the nanotechnologycreated consumer products coming onto the market include a brand of canola cooking oil
called Canola Active Oil, Nanotea (Qinhuangdao Taiji Ring Nano-Products Co., Ltd.,
Hebei, Peoples Republic of China), and a chocolate diet shake called Nanoceuticals Slim
Shake Chocolate (RBC Life Sciences Inc., Irving, TX, USA). The canola oil contains an
additive called nanodrops designed to carry vitamins, minerals, and phytochemicals
through the digestive system and urea. The shake, according to its manufacturer, uses
cocoa-infused NanoClusters to enhance the taste and health benefits of cocoa without
the need for extra sugar. Nanotechnology will replace many fields with tremendous
application potential in the area of dairy and food sectors.
SP-144
NANOTECHNOLOGY AS A MEANS TO ACHIEVE

GOALS IN HORTICULTURE
Swetal Rana1, Rahul Pippal 2 and Payal Rana3
1&2
College of Agriculture, RVSKVV, Gwalior (474020) (M.P.)

3
Krishi College, Jorapali, Raigarh (496001) C.G.
Email: ranaswetal@gmail.com
In human history different materials have been utilized during different times and these
time durations are named accordingly such as Stone Age, Bronze Age and Iron Age etc.
During the last few decades technological advancements are so fast that these have
affected every walk of life. Nanotechnology is such a recent technology that is going to
affect human life in near future in such a big way that we can say that we are moving
towards nano age. Nanotechnology includes manipulation or self-assembly individual
atoms, molecules, or molecular clusters to create materials and devices with new or vastly
different properties. Nanotechnology is the science concerned with the design, synthesis,
characterization, application of materials and devices that have a functional organization in
at least one dimension on the nanometer (nm) scale, ranging from a few to about 100 nm.
Since one nanometer is one billionth of a meter; materials ranging down to nanometer
scale exhibit properties completely different from their bulk counterparts. Nanotechnology
is a multidisciplinary field and has the enormous potential to boost agricultural research.
Recently the science of the materials at the nanometer scale has emerged as one the most
promising subject. Novel materials and surface characteristics might be utilized to enhance
agricultural productivity and production. Nanotechnology can be a tool in better
understanding of various cellular processes, mechanisms regulating important agronomic
traits and development of genotypes tolerant to abiotic and biotic stresses. Nanotechnology
offers better products and improved means of production.
SP-145
STUDIES ON EFFECT OF NANOPRIMING ON MAIZE

(ZEA MAYS L.) SEED GERMINATION
V.K. Chourasiya, P.S. Shukla and K.P. Singh
Department of Genetics and Plant Breeding, Govind Ballabh Pant University of Agriculture and Technology,
Pant Nagar, Udham Singh Nagar-263145 (Uttarakhand)
E-mail: vc3949@gmail.com
Nanotechnology is the creation and utilization of materials devices and systems through
the control of the properties and structure of the matter at the nanometric scale. Targeted
research and development to understand, manipulate and measure of the materials with
atomic, molecular and super molecules dimensions is called nanotechnology.
Nanotechnology has the potential to revolutionize the agriculture with new tools to
enhancing the absorption of nutrients and plant growth regulator by the seed/plants. Seed
is most important input determining productivity of any crop. Conventionally, seeds are
tested for germination and distributed to farmers for sowing. In spite of the fact that seed
testing is done in well equipped laboratories, it is hardly reproduced in the field due to the
inadequate moisture under rainfed conditions. Effects of GA3 loaded silica nanoparticles
on seed germination parameters such as germination percent, dead seed, abnormal
seedling, shoot and root lengths, seedling length, shoot fresh weight, root fresh weight,
seedling fresh weight, shoot dry weight, root dry weight, seedling dry weight and seedling
vigour index maize (Zea mays L.) was probed in the present research. The result showed
that effect of GA3 loaded silica nanoparticles has significant on seed germination and
seedling parameters such as shoot length, root length, seedling length, shoot fresh weight,
root fresh weight, seedling fresh weight, shoot dry weight, root dry weight, seedling dry
weight and seedling vigour index of maize seed. The best Treatment is nano silica with
150ppm GA3. Highest germination percent 91% recorded in 150ppm GA3 loaded silica
nanoparticles treatment. The possible contribution of Silica nanoparticles (SiO2) was to
facilitate the penetration of water and Gibberellic acid through the seed coat and accelerate
the Seed germination and increase growth of seedling.
SP-146
NANOENCAPSULATION OF FOOD INGREDIENTS

Isha Kaushik
PhD Research scholar CCS HAU Hisar
Email id: ishakaushikfst@gmail.com
Nanotechnology is an advanced branch of science impacting a large number of other

fields, including medicine, cosmetics, agriculture, and food. The term nanoencapsulation
describes the application of encapsulation on the nanometer scale with films, layers,
coverings, or simply microdispersion. Nanoencapsulation is an important field of
nanotechnology that involves entrapping of bioactive agents within carrier materials with a
dimension in nano scale to form nano capsules with size ranging from 10 to 1000 nm. In
foods, nanometric materials can change the structure, texture, and quality of foodstuffs
pertaining to improve taste, flavor, color, texture, and consistency of foodstuffs, increased
absorption and bioavailability of nutraceuticals and health supplements, development of
food antimicrobials. New food packaging materials with improved mechanical barrier and
enhanced antimicrobial properties, nanosensors for traceability and monitoring the
condition of food during transport and storagenare other potential applications of
nanometric materials in foods.Some multinational companies, such as Nestl and Unilever,
which are well known worldwide, are also developing functional foods and other products
using the encapsulation techniques. It has enabled the development of food safety and food
quality aspects. By keeping these perspectives, this paper reviewed on the principle of
nanoencapsulation, wall material for encapsulation, methods for manufacturing
nanocapsules and food application of nanoencapsulation.
SP-147
SYNTHESIS AND CHARACTERIZATION OF

NANOPARTICALS
Nisha Devi
Department of Molecular biology, Biotechnology and Bioinformatics
Email: Nishu.sharma57605@gmail.com
Nanoparticles are particles of size ranging from 1 to 100 nanometers. In nanotechnology,

synthesis and characterization of nanoparticals is very important because their
effectiveness depends on size and morphology. A wide range of materials can be used in
synthesis of nanoparticals from plants like Silver (Ag), Gold (Au) and its alloys, Iron (Fe)
and its alloys, Titanium(IV) oxide (TiO2), Zinc(II) oxide (ZnO), Copper (Cu), Palladium
(Pd), Copper oxides (Cu2O and CuO), Indium(III) oxide (In2O3), Lead(II) sulfide (PbS)
etc. Among these silver and gold are most widely used in green synthesis of nanoparticals.
Nanoparticals are characterized by size, shape, surface area and dispersity. These
nanoparticals are characterized by using some common techniques: UVvisible
spectrophotometry, dynamic light scattering (DLS), scanning electron microscopy (SEM),
transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR),
powder X-ray diffraction (XRD) and energy dispersive spectroscopy (EDS). UVvisible
spectrophotometry used for detection of nanoparticals of size from 2-100 nm, DLS tells
about charge distribution over the surface of nanoparticals, while morphological
characterization is done by using SEM and TEM. TEM provides 1000 times better
resolution than SEM. The surface chemistry of particals means identification of organic
functional groups over the nanoparticals is done by using Fourier transform infrared
spectroscopy (FTIR). To know about crystal structure of nanoparticals XRD used while
energy dispersive spectroscopy helps to know about elemental composition of these
nanoparticals. Final aim in characterization is to obtain the nanoparticals with
homogeneity.

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National Conference on Trends in Nanobiotechnology

ROLE OF NANOTECHNOLOGY IN FOOD

1|Abstract & Souvenirs (Supplementary)

National Conference on Trends in Nanobiotechnology

SCREENING OF RHIZOBACTERIA FOR PLANT

Abstract & Souvenirs (Supplementary) |2

National Conference on Trends in Nanobiotechnology

MANAGEMENT OF RICE DISEASES THROUGH

3|Abstract & Souvenirs (Supplementary)

National Conference on Trends in Nanobiotechnology

Abstract & Souvenirs (Supplementary) |4

National Conference on Trends in Nanobiotechnology

MANAGEMENT OF RHIZOCTONIA SOLANI: A

5|Abstract & Souvenirs (Supplementary)

National Conference on Trends in Nanobiotechnology

INFLUENCE OF CHEMICAL PARAMETERS ON

Recognizing the importance of developing eco-friendly methods for the synthesis of

Abstract & Souvenirs (Supplementary) |6

National Conference on Trends in Nanobiotechnology

STRATEGIES TO COMBINE CISGENIC AND

7|Abstract & Souvenirs (Supplementary)

National Conference on Trends in Nanobiotechnology

SALT TOLERANCE BY SILVER NANOPARTICLES

Abstract & Souvenirs (Supplementary) |8

National Conference on Trends in Nanobiotechnology

Development of an efficient experimental protocol for the biosynthesis of nanomaterials is

9|Abstract & Souvenirs (Supplementary)

National Conference on Trends in Nanobiotechnology

Nanotechnology refers to controlling, building, and restructuring materials and devices on

National Conference on Trends in Nanobiotechnology

NANOTECHNOLOGY FOR PLANT MITES

National Conference on Trends in Nanobiotechnology

NANOTECHNOLOGY: NEW FRONTIERS IN

Nanotechnology is science of manipulating materials at nano-scale. It occupies a central

National Conference on Trends in Nanobiotechnology

ROLE OF NANOTECHNOLOGY IN AGRICULTURE

National Conference on Trends in Nanobiotechnology

USE OF NANOMATERIALS IN WHEAT TO

National Conference on Trends in Nanobiotechnology

DECIPHERING THE POTENTIAL OF

Nanotechnology is the Design, Fabrication and Utilization of materials, devices and

National Conference on Trends in Nanobiotechnology

NANOTECHNOLOGY AND SCOPE FOR PLANT

National Conference on Trends in Nanobiotechnology

EXTRACTION & CHARACTERIZATION OF

National Conference on Trends in Nanobiotechnology

MOLECULAR AND NANO APPROACHES FOR

National Conference on Trends in Nanobiotechnology

ROLE OF NANOTECHNOLOGY IN DEVELOPMENT

National Conference on Trends in Nanobiotechnology

National Conference on Trends in Nanobiotechnology

NANOTECHNOLOGY IN CROP PROTECTION

Nanotechnology is the understanding and control of matter at dimensions between

National Conference on Trends in Nanobiotechnology

ADVANCEMENT IN NANODIAGNOSTIC FOR

Former faculty at Department of Sciences (Biotechnology), J. C. D. Memorial College, Sirsa, Haryana

Nanotechnology refers to a nanoscale technology, which has promising applications in

National Conference on Trends in Nanobiotechnology

ROLE OF NANOPARTICLES IN ALLEVIATING

Department of Chemistry and Biochemistry, 1Department of Molecular Biology, Biotechnology and

Nanoparticles have been introduced as materials having good potential to be extensively

National Conference on Trends in Nanobiotechnology

National Conference on Trends in Nanobiotechnology