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Research Article
Analgesic and anti-nociceptive activity of hydroethanolic extract of Capparis decidua
Linn.
1
Abstract
Objectives: To study the analgesic and anti-nociceptive activity of hydro-ethanolic stem extract of Capparis decidua
Lin. Materials and methods: Wistar rats and Swiss albino mice were selected for study of analgesic and antinociceptive activity of Capparis decidua hydro-ethanolic extract (CDHE) at doses 50, 100 and 200 mg/kg p.o. a
variety of models viz. acetic acid induced writhing model (female mice), Eddy's hot plate (mice) and tail ick model for
analgesic study, and formalin-induced paw licking model (mice) were usedforanti-nociceptivestudy. Results: In acetic
acid induced writhing model and in the hot plate model method used with indomethacin standard drug, the maximum
effect was observed at 60 min at a dose of 200 mg/kg p.o., which was higher than the standard drug morphine sulfate
(1.5 mg/kg i.p.) while in the tail ick model, effect was comparable with morphine sulfate. In formalin-induced paw
licking model, administration of CDHE completely abolished the early phase at 100 and 200 mg/kg p.o. and in the late
phase, the result of CDHE (200 mg/kg p.o.) was superior than indomethacin (10 mg/kg p.o.). CDHE was effective in
both non-narcotic and narcotic models of nociception, signifying its possible action via peripheral and central
mechanism. It also abolished the early phase in formalin-induced paw licking model, signifying complete inactivation
of C-ber at higher dose. Conclusion: The activity can be recognized to the phyto-constituents viz tannins, diterpenes,
triterpenes and steroids present in the CDHE extract. In conclusion, CDHE can be developed as a potent analgesic and
anti-nociceptive agent in future.
Keywords:Analgesic, acetic acid, Capparis decidua , Eddys hot plate, hydro-ethanol extract, tail ick
Introduction
The use of herbal plant products is growing in many segments of
the population. According to an estimate 80-90% of the world's
population leading herbal plants for their medicine. The
majority of the synthetic drugs used at present for analgesic and
antinociceptive effect with many side and toxic effects. Herbal
plants still stand for a large untapped resource of structurally
novel active compounds that might serve as lead for the growth
of novel drugs (Ahmad et al., 1992). Numerous medicines of
herbal plant origin with analgesic and anti-nociceptive activity
had been used since long time without any adverse effect. In
north East India is regard as as one of the hotspots for
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Dose
Control
59.4 1.02
143 1.14
Indomethacin
10
23.9 0.44
36.53 2.62
Plant extract
50
21.4 0.01
52.81 1.22
Plant extract
100
0.00
36.76 1.57
Plant extract
200
0.00
26.73 1.11
mg/kg
Values are expressed in terms of mean SEM, n=6 in each group, P 0.01
statically highly significant as compared with control with control group
Conclusions
In conclusion, it can be interpreted that CDHE possesses
shows potential analgesic and anti-nociceptive properties,
which are probably peripherally mediated through
inhibition of prostaglandin synthesis as well as central
bioactive principles.
Table 1. Analgesic activity of CDHE in female albino mice in
acetic acid induced writhing method
Groups
Treatments
Dose mg/kg
No. of writhing
Percentage reduction %
Control
77 1.67
0.00
Indomethacin
10
8.33 0.63
86.45
Plant extract
50
11.8 0.79
80.76
Plant extract
100
6.83 0.83
88.89
Plant extract
200
4.83 0.47
92.14
Values are expressed in terms of mean SEM, n=6 in each group, P 0.01
statically highly significant as compared with control with control group.
Acknowledgments
Authors would like to thank the Management of Lachoo
Memorial College of Science and Technology Pharmacy
Groups Treatments
Jodhpur Rajasthan.
Dose
mg/kg
30 min
60 min
90 min
120 min
Control
4.990.25
5.030.09
5.700.15
5.310.35
4.690.20
Morphine
1.5
8.550.25
10.10.09
12.30.15
13.80.35
14.90.20
Plant extract
50
8.800.89
11.70.65
13.70.63
14.40.85
16.50.88
Plant extract
100
12.00.66
14.40.38
15.90.33
17.60.74
15.40.45
Plant extract
200
13.60.52
15.60.81
18.20.64
15.20.48
14.60.53
Values are expressed in terms of mean SEM, n=6 in each group, P 0.01
statically highly significant as compared with control with control group.
Control
Dose
mg/kg
0 min
30 min
60 min
90 min
120 min
Morphine
1.5
Plant extract
50
Plant extract
100
Plant extract
200
Values are expressed in terms of mean SEM, n=6 in each group, P 0.01
statically highly significant as compared with control with control group.
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pp 385.
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