Académique Documents
Professionnel Documents
Culture Documents
ALBUMIN
B.C.G. colorimetric test
ORDER INFORMATION
Cat. No.
ALB 100
ALB 200
LINEARITY
Kit Size
2x50 ML
2x100 ML
AUTOMATED PARAMETERS
Wavelength
Cuvette
Reaction Temperature
Measurement
Reaction
Reaction Direction
Sample / Reagent Ratio
Incubation
Low normal
High Normal
Linearity
ASSAY PROCEDURE
PIPETTE INTO TEST TUBES
BLANK
STD
SAMPLE
5 l
Sample
PRINCIPLE
The measurement of serum albumin is based on its quantitative binding to
the indicator 3, 3, 5, 5 - tetrabromo-m-cresol sulphophthalein
(bromocresol green, BCG). The albumin-BCG complex absorbs maximally
at 578 nm.
REAGENT COMPOSITION
Reagent I
:
Albumin standard :
BCG reagent
4 g/dl (store at 2-8C)
620 nm
1 cm light path
Room Temperature
Against Reagent Bl
End Point
Increasing
1 : 200
5 minutes
1.8 g/dl
5.1 g/dl
8.0 g/dl
Standard
5 l
Reagent
1000 l
1000 l
1000 l
Mix well, and wait for 5 mins at Room Temperature. Measure the
absorbance of the Sample (Ac) and Standard (As) against the reagent
blank.
CALCULATION
Ac/As x Conc. Std. = g/dl Albumin
BIBLIOGRAPHY
E.M. Gindler and J. O. Westgard Clin. Chem., (1973), 6,4.
J.O. Westgard, M.A. Poquette, Clin. Chem., (1973) 19, 647.