Académique Documents
Professionnel Documents
Culture Documents
Abstract
WHO has identified more people with tuberculosis disease. Indonesia is the third country
with tuberculosis patients. Mycobacterium tuberculosis as a cause of tuberculosis has been resistant
to antituberculosis drugs. In previous research proved that the ethanol extract of mimba cortex can
inhibit growth of Mycobacterium tuberculosis based on in vivo toxicity test on guinea pigs infected by
Mycobacterium tuberculosis H 37 Rv. The previous results of toxicity test is mild toxic category. This
study aimed to determine the antituberculosis potency of ethanolic extract of mimba cortex (EEM)
on tuberculosis patients. As many as 30 patients were divided into 3 groups, each consisting of 10
patients. Group I patients were given by FDCs for tuberculosis treatment category I for intensive
phase. Group II patients were given by FDCs for tuberculosis treatment category I for intensive phase
and 2 capsules of EEM 100 mg each day. Group III patients were given by FDCs for tuberculosis
treatment category I for intensive phase and 3 capsules of EEM 100 mg each day. Antituberculosis
assay was done by taking patients sputum speciments every two weeks and tested using LowensteinJensen method. After two months of treatment for stage I, the treatment will be continued by
advanced FDCs, and evaluated every two weeks for 4 months. The result showed that FDCs for
tuberculosis treatment in combination with 3 capsules of EEM in each day showed negative result of
Mycobacterium tuberculosis in week six, meanwhile the combination of FDCs for tuberculosis
treatment with 2 capsules EEM each day showed similar result with the treatment of FDCs only to
gain negative result of Mycobacterium tuberculosis in week eight, and the treatment with advanced
FDCs showed negative result of Mycobacterium tuberculosis after advanced four weeks.
Keyword : mimba cortex, antituberculosis, tuberculosis patients.
INTRODUCTION
Tuberculosis (TB) is still the main health
problem in the world. Everyone tried to prevent
TB. Even Bill Gates and George Soros funding
organization which called GF ATM (Global Fund
against AIDS, TB and Malaria) gave fund to
Indonesian tuberculosis prevention to get in
charge in tuberculosis prevention (DepKes RI,
2008). Various improvements in tuberculosis
prevention programs have been reached, such as
Directly Observed Treatment (DOTS) program.
Indonesia almost reached DOTS program target
of 70/85, which means at least 70% of TB patients
in Indonesia have been found and at least 85% of
them have been cured. Indonesia was also
introduced to some programs, such as HDL
(Hospital DOTS Linkage) that holds DOTS
program in hospitals, PPP (Public Private
Partnership) or PPM (Public Private Mix) which
involving private sectors on TB prevention.
59
Fatimah et al.
ISSN: 2088 0197
e-ISSN: 2355-8989
60
Fatimah et al.
ISSN: 2088 0197
e-ISSN: 2355-8989
thyposa,
Streptococcusaureus
(Ambarwati,
2007), Streptococcus mutant and Streptococcus
faecalis (Almas, 1999). Mimba oils can also
inhibit the growth of E. coli and Kleibsiella
pneumonia (Sai Ram et al., 2000). Besides, the
etanolic extract of mimba cortex can inhibit the
growth of Mycobacterium tuberculosis (Fatimah,
2012).
Table 1. The result of treatment with FDCs for
tuberculosis treatment category I for intensive phase
without capsules of EEM
Category of sputum analysis result
Patient
code
Replication
number
I
II
III
IV
I.1
3+
2+
2+
1+
I.2
3+
2+
I.3
3+
2+
2+
1+
I.4
3+
2+
2+
1+
I.5
3+
1+
1+
I.6
3+
1+
I.7
3+
2+
I.8
3+
2+
1+
I.9
3+
2+
1+
I.10
3+
2+
1+
Fatimah et al.
ISSN: 2088 0197
e-ISSN: 2355-8989
II
III
IV
II.1
3+
2+
2+
1+
II.2
3+
2+
1+
II.3
3+
2+
II.4
3+
2+
2+
1+
II.5
3+
2+
II.6
3+
1+
1+
II.7
3+
2+
2+
1+
II.8
3+
2+
II.9
3+
2+
1+
II.10
3+
1+
1+
II
III
IV
III.1
3+
1+
III.2
3+
1+
III.3
3+
1+
III.4
3+
1+
III.5
3+
1+
III.6
3+
1+
III.7
3+
1+
III.8
3+
1+
III.9
3+
1+
III.10
3+
1+
Category of sputum
analysis result
Replication
I
II
III
I.1
1+
1+
I.3
1+
I.4
1+
Category of sputum
analysis result
Replication
I
II
II.1
II.4
1+
II.7
3+
62
Fatimah et al.
ISSN: 2088 0197
e-ISSN: 2355-8989
CONCLUSION
Based on this study, the treatment of
EEM capsule as co-chemotherapy for FDCs for
tuberculosis treatment showed good potential as
antituberculosis on TB patients. The data showed
that 1) the treatment by the combination of 3
capsules of EEM with FDCs category I for
intensive phase in each day could change the
condition of +3 categorized patients to be
categorized as negative after 6 weeks of
treatment. 2) The treatment by using 2 capsules of
EEM with FDCs category I for intensive phase in
each day showed that from the patients detected
ACKNOWLEDGEMENT
We acknowledge DP2M DIKTI who
grant this study through Competitive Grants
Research 2014, the head of Lung Disease Clinic,
Sumatera Utara, the head of Local Health
Laboratory, Sumatera Utara, and also the head
and staff of Phytochemical Laboratory, Faculty of
Pharmacy Universitas Tjut Nyak Dhien Medan.
REFERENCES
Dalimatha, Setiawan, 2000, Atlas Tanaman Obat
Indonesia, Trubus Agriwidya, I(I), Jakarta.
Depkes RI, 1995, Materia Medika Indonesia, 4th
edition,
Departemen
Kesehatan
Republik Indonesia, Jakarta, 297307,322-339.
DepKes
RI,
2008,
Pedoman
Nasional
Penanggulangan
Tuberkulosis,
Departemen
Kesehatan
Republik
Indonesia, Jakarta
Kardinan, A. dan Taryono, 2003, Tanaman obat
penggempur kanker, PT. Agromedia
Pustaka, Jakarta.
Misnadiarly, 2006,
Tuberkulosis
dan
Mikobakterium Atipik., PT. Dian Rakyat,
Jakarta, 98-100.
Rembold, T., 1989, Azadirachtin, their structure and
mode of action. Federal Republic of
Germany Press. 15, 1639-1643,
Germany.
63