Colleen Gallagher

EECE 2160
Professor Mark Niedre

Lab #8B: EOG

Intro/Objectives:
In this lab, the goal is to further experiment with different biomedical applications
utilizing the concepts we learned throughout the semester. Specifically, we will be
building an EOG or rather an electro-oculogram. This measures the angular horizontal
position of the eyes by measuring the resting corneal-retinal bio-potential. EOGs can be
used to detect REM sleep during sleep staging. This lab utilizes the circuit built in labs 6
and 7 with the AD627 instrumentation amplifier without the high pass filter. As well as
utilizing what we have learned throughout the semester, we will be developing our circuit
trouble shooting and design skills and learning about a new application.

Results/Analysis:
Part 1:
1.3) In order to get a gain of 25, we used an Rg value of 10 k:
200 k
200 k
gain=25=
+ 5=
+5
Rg
10 k
1.5) Given that the EOG has an approximate frequency range of 0 to 10 Hz, we used an
Rf value of 1.6 k, a Cf value of 10 F, and a Rs value of 1 k. Based on the frequency
range, the cut frequency is 10 Hz. In order to calculate the appropriate resistor and
capacitor values, we used these calculations:
f
1
( c2 )=
=0.0159
(102 )
1
=

=Rf Cf =( 1.6 k ) ( 10 k )=0.016

The resulting gain caused by this low pass filter can be calculated by:
Rf 1.6 k
gain= =
=1.6
Rs
1k

1.6) Yes, the output was what we expected. We saw a sine wave with a peak to peak with
amplitude of about 1.9 V, which means that the original signal was in fact amplified by
factor of 40.
1.7) The theoretical gain of the combined circuit is the gain of the instrumentation
amplifier multiplied by the gain of the low pass filter:
theoretical gain=251.6=40
The actual gain of this circuit can be found by dividing the output voltage by the input
voltage:
1.5 V
actual gain=
=30
0.05V
1.8) To find the cutoff frequency, we multiplied 0.707 by the calculated gain of 40 to find
the corresponding gain of 28.28. The next step is to adjust the frequency of the input
signal until the gain matches this value by looking at the oscilloscope, which was about 9
Hz. At this frequency, the input was 50 mV and the output was 1.4 V, which yields a gain
of around 28.
1.4 V
=28
0.05V

Part 2:
2.1) Example Trace: (10,000 scans at a 1000 sampling frequency = 10s)

Looking at the graph, we can see that my partner starting looking left (positive), forward
(zero), to the right (negative), and then back to looking straight ahead. This sequence was
repeated twice in the 10 second time span acquired in Matlab. The second 5 second time
period was not as neat and there is some extra waves most likely due to the fact that he
eye movements werent as smooth.
2.2) Tracking Video #1: (30,000 scans at a 1000 sampling frequency = 30s)

Looking at this graph, we can see that the eye movements were a lot smoother without
much pausing in between movements as seen in the previous tracking example. The
signal looks like a sine wave. My partner was successfully able to follow the movement
of the object in the video, which was moving relatively slowly from left to right. We can
determine the frequency of the moving object from the EOG by taking the inverse of the
period of the signal (each sample is about 1 second):
1
1
1
f= =
=
=1.05 Hz
T (1.250.3) 0.95
2.3) Tracking Video #2: (30,000 scans at a 1000 sampling frequency = 30s)

Finally, looking at the final graph, we can see that the eye movements were a lot quicker
than the previous examples and also varied in value in terms of where the signal was
centered on (x-axis). This sort of fluctuation was most likely caused by movement of
other body parts (such as the head or neck) rather than just strictly the eyes. My partner
was successfully able to follow the movement of the object in the video, which was
moving relatively quickly from left to right. We can determine the frequency of the
moving object from the EOG by taking the inverse of the period of the signal (each
sample is about 1 second):
1
1
1
f= =
=
=5 Hz
T (0.70.5) 0.2

Conclusion:
From this lab, I gained a better understanding of combining all of the concepts we
have learned throughout the semester as well as modifying a circuit in order to satisfy the
design goals. Similar to previous labs, the concept that perfect results are not always the
most realistic was reinforced, but we managed to acquire acceptable signals through our
implementation of this circuit as well as utilize these techniques for a different
biomedical application. In addition, Matlab data analysis techniques were expanded on
and used to interpret the eye movement signals. There werent many key lessons learned,
but rather more so the application of other key lessons as well as learning how to debug
the circuit to produce a signal that was clear and accurate to interpret eye movement.