Introduction

Vitamin C also called ascorbic acid, is an

essential nutrient for humans and other
animals
Vitamin C is needed by the body to form
collagen. body also uses vitamin C to
make skin, tendons, ligaments and blood
vessels. It also uses this vitamin to repair
and maintain cartilage, bones and teeth,
to heal wounds and to form scar tissue.
A person needs at least 40mg vitamin
C daily. The lack of vitamin C in the body
can cause weak immunity, and even
scurvy.

Thus ,It is important to know how

much vitamin C is in the food we eat.

I would like to express my special thanks of

gratitude to my teacher MR Sunderash sir as
well as our lab assistant Mrs. Fatima who
gave me the golden opportunity to do this
wonderful project on the topic (Write the
topic name), which also helped me in doing
a lot of Research and i came to know about
so many new things I am really thankful to
them.
Secondly i would also like to thank my
parents and friends who helped me a lot in
finalizing this project within the limited time
frame.

Aim

To determine the amount of vitamin-c

(absorbic acid) present in different
fruitjuices by titration with iodin solution and
compare its value with the mentioned
values on on their bottle lables

Theory
It's well known that Iodine reacts with
many substances as it is very powerful
oxidant. In the mixture of many different
substances it first reacts with molecules
that easier to break down.
Vitamin C is anti-oxidant and will react
with Iodine promptly. If we mix vitamin C
and starch and will gradually add iodine
into this mixture, iodine will only react
with vitamin C, ignoring starch
When mixture ran out of vitamin C
Iodine will react with starch. This reaction
has very distinctive dark blue color
output. As soon as all the vitamin C
reacts with iodine next drop of iodine will
react with starch and turn the mixture
into dark blue.

 Thus we can find out the exact

amount of iodine needed to react with
vitamin C. Knowing that we can calculate
amount of vitamin C in the mixture.

Formula used
Amount of vit.c : vol. of iodine used for
juice
mg/ml
samples

Absorbic value

Titration process at the beginning and

almost the end

Materials required

Measuring cylinder
Titration cylinder
Beaker
Burette
Burner
Matchbox
Titration box
Iodine solution
Starch solution
Vitamin c solution
3 fruit juices


Preperations
Take a one liter beaker and fill it up with 500
ml of water.
Crush one (500mg) or two (250 mg) tablet
of vitamin C and put it in the jar, stir it well,
until vitamin C tablet dissolves.
This way you'll get 1 mg/ml solution of
vitamin C.
Measure 20 ml of that solution, bring it to
another glass and add about half glass of
water.
This glass will contain 20 mg of vitamin C.
Starch solution
Starch indicator solution: (0.5%). Weigh 0.25
g of
soluble starch and add it to 50 mL of near
boiling water

in a 100 mL conical flask. Stir to dissolve

and cool before
using.

Procedure
20 ml of the prepared vitamin c solution is
measured using a measuring cylinder and
taken on a conical falsk,2 ml of prepared
starch solution is added to this
2 well cleaned burette is rinsed with iodine
and is filled withit
The experimentation set up is made with
burette and conical flask in a stand
The iodine solution is slowly run into he
conical flask,and it is shaken and rotated
slowly
The endpoint of the titration is identified as
the first
permanent trace of a dark blue-black colour
due to
the starch-iodine complex.
At this stage burette reading is noted as
final burette reading .yhis value indicates

the amount of iodine solution needed to

react with 20mg of vitamin c

Titration of fruit juices

20 ml of the prepared vitamin c solution is

measured using a measuring cylinder and
taken on a conical falsk,2 ml of prepared
starch solution is added to this
2 well cleaned burette is rinsed with iodine
and is filled withit
The experimentation set up is made with
burette and conical flask in a stand
The iodine solution is slowly run into he
conical flask,and it is shaken and rotated
slowly
The endpoint of the titration is identified as
the first
permanent trace of a dark blue-black colour
due to
the starch-iodine complex.
At this stage burette reading is noted as
final burette reading .yhis value indicates
the amount of iodine solution needed to
react with 20mg of vitamin c

Calulations
For titration

for