Name: Elizabeth Jade Vicera

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 2: Simulated Facilitated Diffusion Lab Report

Pre-lab Quiz Results

You scored 75% by answering 3 out of 4 questions correctly.

1. Molecules need a carrier protein to help them move across a membrane because
Your answer : a. they are not lipid soluble.
Correct answer: d. they are lipid insoluble or they are too large.

2. Which of the following is true of facilitated diffusion?

You correctly answered: c. Movement is passive and down a concentration gradient.

3. Examples of solutes that might require facilitated diffusion include

You correctly answered: d. all of the above.

4. Which of the following would not affect the rate of facilitated diffusion?
You correctly answered: a. the amount of intracellular ATP

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Experiment Results
Predict Question:
Predict Question 1: What effect do you think increasing the number of protein carriers will have on the glucose transport
rate?
Your answer : b. The glucose transport rate will increase.

Predict Question 2: What effect do you think adding Na+ Cl- will have on the glucose transport rate?
Your answer : b. The glucose transport rate will decrease.

Stop & Think Questions:

What happened to the glucose transport rate when the glucose concentration was increased from 2 mM to
8 mM?
You correctly answered: a. The glucose transport rate increased.

Describe the conditions that resulted in the fastest rate of glucose transport.
You correctly answered: d. 700 glucose carrier proteins and 8 mM glucose

Why do you think equilibrium wasn't reached?

You correctly answered: b. The membrane proteins were saturated.

Experiment Data:

Run Number Solute Start Conc. L Start Conc. R Carriers Rate

1 Na+ Cl- 0.00 0.00 500 0.0000
1 Glucose 2.00 0.00 500 0.0008
2 Na+ Cl- 0.00 0.00 500 0.0000
2 Glucose 8.00 0.00 500 0.0023
3 Na+ Cl- 0.00 0.00 700 0.0000
3 Glucose 2.00 0.00 700 0.0010
4 Na+ Cl- 0.00 0.00 700 0.0000
4 Glucose 8.00 0.00 700 0.0031
5 Na+ Cl- 0.00 0.00 100 0.0000
5 Glucose 10.00 0.00 100 0.0028#
6 Na+ Cl- 0.00 2.00 700 0.0042
6 Glucose 2.00 0.00 700 0.0010

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. What variable(s) increased the rate of facilitated diffusion of glucose?

You correctly answered: d. both a and b

2. For facilitated diffusion, increasing the concentration of glucose on one side of the membrane is the same as
You correctly answered: b. making the concentration gradient steeper

3. When all of the membrane carriers are engaged, or busy, we say they are
You correctly answered: a. saturated.

4. Na+ Cl- had no effect on glucose transport because

You correctly answered: b. sodium is not required for glucose transport in the simulation.

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Review Sheet Results
1. Explain one way in which facilitated diffusion is the same as simple diffusion and one way in which it is different from
simple diffusion.
Your answer:
Facilitated diffusion is the same as simple diffusion such that both exhibits transport in a high concentration gradient to a low
concentration gradient. Facilitated diffusion is different from simple diffusion because in order to increase the rate of the
diffusion, both the membrane carriers and the volume should be increased while in simple diffusion, increasing only the
volume can speed up the diffusion.

2. The larger value obtained when more glucose carriers were present corresponds to an increase in the rate of glucose
transport. Explain why the rate increased. How well did the results compare with your prediction?
Your answer:
The rate increased since there will be more transport proteins present in the membrane, thus, holding more molecules to be
transported along the concentration gradient. It makes the gradient steeper. The results correctly matched my prediction.

3. Explain your prediction for the effect Na+ Cl- might have on glucose transport. In other words, explain why you picked
the choice that you did. How well did the results compare with your prediction?
Your answer:
I picked it thinking that they might be correlated and that adding NaCl would decrease the rate since it will be an addition to
the gradient. The results showed otherwise, that glucose transport is independent of NaCl transport since NaCl exhibits
simple diffusion so it doesn't need transporters in order to diffuse fast while glucose transport needs transporters/carriers in
order to increase the diffusion rate.

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