Continuous Chromatography

At Novasep, we can
reduce the cost
of your chiral separations

Michel Abrial
Production

Varicol 6 x 300

Varicol 6 x 1000

Prochrom Varicol Systems

Multicolumn Continuous Chromatography
for cost-effective chiral separations of kg to multi-ton scale

www.novasep.com
 Varicol Multicolumn Continuous Chromatography Offering

Based on our unique experience in preparative and industrial chromatography, we have developed a robust and
efficient line of Varicol multicolumn continuous chromatography systems, a substantial improvement of the well-
known simulated moving bed (SMB) technology.
Particularly well adapted for kilogram up to multi-ton binary separations, our Varicol systems suit any chiral
compound resolution, isomer and other binary mixture separation.
Our Varicol systems are used for the production of all the
commercial APIs sold as pure enantiomers and manufactured by
chiral chromatography.
Our Varicol offering, backed by our expert process development
services, includes:
Prochrom Varicol chromatography systems
Prochrom DAC columns
Solvent storage and recycling systems

Our solutions for multicolumn continuous chromatography offer:

Cost effectiveness Prochrom Varicol 6 x 200

Prochrom Varicol is an advanced continuous chromatography process which is 2 to 10 times more productive
than a batch process, such as HPLC, for large scale binary separations; providing the highest productivity, best
return on investment as well as the lowest operating costs. Our systems fully integrate solvent recycling to
reduce your solvent consumption by up to 99.97%(1). Since 2007, Novasep and Daicel/Chiral Technologies offer a
joint approach to combine our expertise in process development with their expertise in chiral stationary phases to
ensure that your process is optimally developed for guaranteed performance.

Time efficiency
Our chemists efficiently design the right chromatographic separation method for your intermediate or API using
Novaseps proprietary computer simulation platform, which has been continuously developed for 20 years.
The guaranteed scalability and robustness of our chromatographic processes will ensure the quickest delivery of
your API for clinical supplies, process validation, registration and commercial production.

Ease of use
Varicol systems are entirely automated and require minimal operator interface. Fully compliant with the PAT
(Process Analytical Technologies) guidelines of the FDA, the ACS (Advanced Control System) automatically
controls your Prochrom Varicol system and increases separation performance and robustness.

Principle

In multicolumn continuous chromatography, one long column is

replaced by a loop of 4 to 6 shorter columns, optimizing the
usage of the stationary phase. Only pure fractions are collected,
leaving mixed fractions to re-circulate through the columns.
Contrary to SMB, Prochrom Varicol is a features non-
synchronous shifting of the inlet and outlet lines. The number
of columns periodically varies in each zone of the system. This
ensures a more efficient use of stationary phase and a higher
productivity compared to SMB. In most cases, Varicol is 15 to
30% more productive than SMB and typically 5 or 6 columns
are used in Varicol whereas 6 to 8 columns are used in SMB.
Prochrom Varicol 6 x 600

(1) Production data from UCB Pharma

 Prochrom Varicol Chromatography Systems

Our fully automated Varicol systems are designed for process development, pilot-scale and industrial chromatographic
separations. The proven design of our Varicol systems will meet your specific process needs:
Optimal industrial design allowing 24/7 continuous operation
High productivity and yield resulting from excellent process reproducibility
Reliable instrumentation and easy access for reduced maintenance
Validated (based on GAMP 5 and compliant with 21 CFR part 11) and user-friendly software
Fully PAT compliant with our advanced control system

Prochrom Varicol Laboratory Pilot Industrial

LAB LAB 6-110
Type Micro 6-200 6-300 6-450 6-600 6-800 6-1000
(30-50) (50-80) 6-150
Column diameter (mm) 10 30 - 50 50 - 80 110 - 150 200 300 450 600 800 1000

g/day kg/week tons/year

Productivity range
10 - 40 50 - 1000 250 - 2500 10 - 200 40 - 400 90 - 900 8 - 80 15 - 150 25 - 250 40 - 400

Stationary phase (kg) 0.05 0.4 - 1 1-3 3-6 10 25 55 100 180 280

All our Varicol systems are designed to be operated under cGMP conditions. They are fully qualified and extensively
tested during factory acceptance testing (FAT) and site acceptance testing (SAT).

Prochrom DAC Columns for Varicol Systems

Dynamic Axial Compression (DAC) provides the optimum packed bed density
and stability, thereby ensuring optimum separation efficiency and consistent
performance for an extended period of time (up to several years corresponding
to stationary phase lifetime).
The column includes a movable piston attached to a hydraulic jack. The piston is
used to pack and unpack the column and keep the stationary phase under dynamic
compression, ensuring the highest efficiency and bed stability at any scale.
For laboratory scale systems (50 or 80 mm column diameter), Pack-n-Sep
columns are available. They combine the advantages of both Prochrom DAC
columns and an ultra fast packer with column packing or unpacking performed in
200 mm Varicol columns
less than 5 minutes. Only one packer is needed to pack/unpack all columns
required for your Varicol separation.

Integration of Solvent Storage and Recycling

At large scale, solvent recycling is mandatory to minimize

solvent consumption and thus reduce both associated costs and
environmental impact.
We have developed efficient solvent storage and recycling
solutions fully integrated within our Varicol chromatography
systems. Depending on your scale of operation and molecule/
process constraints, our solvent recycling solutions may include
automated rotary, wiped or falling film evaporators and/or distillation
columns.
 Copyright and Trademark Information: Groupe Novasep SAS and/or its affiliates (hereafter Groupe Novasep) own or licence the copyrights, trademarks, names, logos, and other rights to the information on this brochure. No right or licence is granted to any other party.
Any unauthorized use without the express prior written consent of Groupe Novasep is prohibited. Disclaimers: The information contained on this brochure are provided as is, for informational purposes only, without any representation or warranty of
Expert Process Development Services

Whether you need to develop a complete purification

process, a multi-step synthesis or a single
chromatographic step, our teams of chemists,
biochemists and process engineers will provide you with
a complete and guaranteed solution.

accuracy or completeness. In no event will Groupe Novasep be liable to any party for any damagesor any other losses fro access or reliance upon any information contained on this brochure. Novasep 2010
We develop your process
We design customized chromatography units that
suit your facilities and specific process needs
We provide process performance guarantees for
our standard and customized systems

Varicol Lab

Full Customer Service for your Varicol Systems

More than 2,000 Novasep laboratory and production chromatography units are installed worldwide for the
production of pharmaceuticals, biopharmaceuticals and fine chemicals.

Local Technical Support Services


Customer service teams in France, the USA & China
for prompt and courteous service, when and where
you need it

Local inventory of standard parts to ensure quick
delivery

Training Services & Expertise


Tailor-made, on-site training provided by our
experienced technical staff to help you maintain
optimum process performance
Performance optimization for new or existing equipment

Novasep combines the expertise of specialists in purification processes

and chemical synthesis. We strive to provide our customers with the best solution
for obtaining pure active molecules.

EUROPE - Novasep Process NORTH AMERICA - Novasep Inc. REST OF THE WORLD - Novasep Process
Phone: +33 (0)3 83 49 70 00 Phone: +1 610 494 0447 Phone: +33 (0)3 83 49 70 00
varicol@novasep.com varicol.north-am@novasep.com varicol@novasep.com

ASIAN COUNTRIES - Novasep Asia INDIA - Novasep Partner, Nishotech JAPAN - Novasep Japan K.K.
Phone: + 86 21 6045 1600 Phone: +91 22 41515151 Phone: +81 03 3221 1960
varicol.asia@novasep.com varicol.india@novasep.com japan@novasep.com

www.novasep.com
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