BIOL 2104 (CRN 91011) Fall 2013

CELL & MOLECULAR BIOLOGY: Quiz No. 2, 01 October 2013

Answer sheet for Quiz #2. Please note that your quiz may not look exactly like this one because
the order of rows and columns differed. Comment [JT1]: This quiz emphasized vocabulary.

For the two following sentences, use the best word or phrase from the lists below. Not all
words or phrases may be used; each word or phrase should be used only once.
1) (4 pts total, 0.5 pts each) Place the most appropriate terms describing mRNA transcription
into appropriate box matching the row and column requirements Comment [JT2]: Some terminology of prokaryotic and
eukaryotic transcription differs.
Transcription for mRNA Eukaryotic Prokaryotic
Initiation site signal TATA box Promoter
Enzyme RNA Polymerase II RNA polymerase
Accessory proteins General transcription factors Sigma factor
Termination site signal Polyadenylation signal Terminator
Polyadenylation signal Terminator Sigma factor
RNA polymerase Promoter General transcription
RNA polymerase II TATA box factors

2) (2 pts total, 0.5 pt each) The genetic code is based on the unit called the ___codon___ which
has three positions for one of four bases. Because there are only 20 amino acids, there are
__redundancies_ in the genetic code that are associated often with the third position
__wobble__. To establish the correct reading frame in eukaryotic mRNA, the ribosome
hybridizes the _initiator tRNA_ to the first AUG after the 7-methylguanosine at the 5-end of
the message.
Initiator tRNA Wobble Translations
Codon Anticodon
Redundancies Stop

3) (3 pts total) Are these terms associated with Eukaryotes, Prokaryotes, or Neither.
Ribosomal protein _x 0.25 pt_ _ x 0.25 pt _ _____
Operon _____ _ x 0.5 pt _ _____
Capping factor _ x 0.5 pt _ _____ _____
Polycistronic mRNA _____ _ x 0.5 pt _ _____
Sigma factor _____ _x 0.5 pt_ _____
Promoter _ x 0.25 pt _ _ x 0.25 pt _ _____
4) (1 pt) Polymerase chain reaction refers to the technique of amplifying DNA sequences in a
living cell. True or False
False PCR is a technique to amplify (make more copies) of DNA sequences in a test tube.