Celldyn Troubles

CELL-DYN 3000/3500/3700/3200
TROUBLESHOOTING GUIDE
Abbott Diagnostics
A Division of Abbott Laboratories
Abbott Park, IL 60064
CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide
ii 67483-101
REVISION STATUS
Revision History Pages Revised and Added

67483-101 6/2002 First Release
CELL-DYN is a registered trademark of Abbott Laboratories.

This guide was developed and produced by TLO Learning Services in Irving, TX.
Copyright 2002, Abbott Laboratories

67483-101 iii
Notes

67483-101 iv
CELL-DYN 3000/3500/3700/3200
TROUBLESHOOTING GUIDE
CONTENTS
RBC Diluent Syringe Overpressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
HGB Lyse Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
WBC Lyse Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Lyse Empty (CELL-DYN 3500/3700) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Diluent/Sheath Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000) . . . . . . . . . . . . . . . . . . . . . . . . 9
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700) . . . . . . . . . . . . . . . . . . . 11
Shear Valve Position Fault . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Vacuum Accumulator Wet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Clog Error (RBC or WIC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Flow Error (RBC or WIC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
WOC / WBC Flow Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Elevated PLT Backgrounds / LRI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Controls Out of Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Controls Out of Range - WBC / WOC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Controls Out of Range - NOC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Controls Out of Range - WIC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Controls Out of Range - RBC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Controls Out of Range - HGB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Controls Out of Range - MCV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

67483-101 1
Controls Out of Range - PLT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
WIC / WOC Mismatch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
H & H Mismatch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Incomplete Aspiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Increased RRBC Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Background Data Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Data Not Transmitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700 . . . . . . . . . . . . . . . . . . . . 55
WOC Flow Cell Cleaning Procedure (CELL-DYN 3000/3500/3700) . . . . . . . . . . . . . . . . . . . . 59
WOC Flow Cell Cleaning Procedure (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

2 67483-101
RBC Diluent Syringe Overpressure
RBC DILUENT SYRINGE OVERPRESSURE

RBC Diluent
RBC Diluent
Syringe
Syringe
Overpressure
Overpressure
Check center
section of shear
valve
Remove and reinstall

Is notch in Shear Valve & release
correct No pressure from RBC
position? Diluent Syringe (See
Note) Run backgrounds
Yes
Check RBC
dilution pathway Run Does error
No Problem resolved
for pinched/ backgrounds recur?
crimped tubing
Yes
Pinched/ Refer to HS or
crimped tubing dispatch service
seen?
Yes
Remove crimps/
massage tubing
Go to Diagnostics,
More, Initialization
Run backgrounds
No
Does error
No Problem resolved
recur?
NOTE:
Remove RBC diluent syringe from
bracket (do not disconnect tubing)
Yes
y Pull down slightly on plunger
y Reinitialize analyzer by going
to: Remove, clean, and
- Main reinstall shear valve &
- Diagnostics release pressure from Does error
No Problem resolved
- More RBC diluent syringe recur?
- Initialization (See Note) Run
y If cannot initialize, cycle power backgrounds
and reinitialize
Yes
Clean/replace
Refer to HS or Does error
Yes RBC Diluent
dispatch service recur?
Syringe
No
Problem resolved

67483-101 3
HGB Lyse Empty (CELL-DYN 3200)
HGB LYSE EMPTY (CELL-DYN 3200)

HGB Lyse Empty
HGB Lyse3200)
(CELL-DYN Empty
(CELL-DYN 3200)
Press CLEAR
FAULT and
observe HGB Lyse
Syringe
Is syringe
Clean or replace Does error
moving No No Problem resolved
syringe recur?
properly?
Yes
Yes
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom? Dispatch Service
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Disable analyzer,
Is syringe filled exercise valve 24
properly? Are using valve puller Does error
No
reagent lines not recur?
crimped? Massage and reseat
tubing in valve 28
Yes
Yes
Refer to HS or Cycle power

dispatch service
Does error
Dispatch Service Yes No Problem resolved
recur?

4 67483-101
WBC Lyse Empty (CELL-DYN 3200)
WBC LYSE EMPTY (CELL-DYN 3200)

WBC Lyse Empty
WBC Lyse3200)
(CELL-DYN Empty
(CELL-DYN 3200)
Press CLEAR
FAULT and
observe WBC
Lyse Syringe
Is syringe
Clean or replace Does error
moving No No Problem resolved
syringe recur?
properly?
Yes Yes
Check reagent cube and inlet lines: Dispatch Service

- cube empty?
- reagent correct?
- inlet line reaches to bottom?
Disable analyzer, and

exercise valve
Is syringe filled
25 using valve puller
properly? Are Does error
No No Problem resolved
reagent lines not recur?
Massage and reseat
crimped?
tubing in valve
(normally closed) 23
Yes
Yes
Refer to HS or
dispatch service
Cycle power
Does error
No Problem resolved
recur?
Yes
Dispatch Service

67483-101 5
Lyse Empty (CELL-DYN 3500/3700)
LYSE EMPTY (CELL-DYN 3500/3700)

L y s e E m p ty
( C E L L - D LYyNs e3 5E0m0 p/3t y7 0 0 )
(C E L L -D Y N 3 5 0 0 /3 7 0 0 )
C h e c k r e a g e n t c u b e a n d in le t lin e s :
- c u b e e m p ty ?
- r e a g e n t c o r r e c t?
- in le t lin e r e a c h e s to b o tto m ?
- v e n t h o le in c a p is o p e n ?
- fo a m s e a l r e m o v e d fr o m u n d e r c a p ?
- lin e s n o t c r im p e d ?
- p o r ts n o t p lu g g e d ?
- c h e c k flu id le v e ls in s y r in g e
P r im e ly s e t o w a s t e c h a m b e r
2 b y r e m o v in g t u b in g f r o m
P re s s C L E A R F A U L T V 3 6 (N C V )
F lo s s tu b in g in to N C V 3 6
E x e r c is e s o le n o id s D o e s e rro r
Yes
42, 47 re c u r?
No
D o e s e rro r
No P r o b le m r e s o lv e d
re c u r?
Yes
C h e c k W IC /H G B L y s e S y r in g e :
- s e a te d a n d m o v in g c o r r e c t ly ?
- tu b in g c r im p e d ?
C le a n /r e s e a t s y r in g e a s n e e d e d
a n d r e m o v e c r im p s
D o e s e rro r
No P r o b le m r e s o lv e d
re c u r?
Yes
C h e c k ly s e in le t lin e fo r c r im p s No
o r p lu g s
R e m o v e ly s e in le t tu b in g & f lu s h
lin e w ith w a rm D I w a te r
D o e s e rro r D o e s e rro r
P r o b le m r e s o lv e d No Yes C y c le P o w e r
re c u r? re c u r?
Yes
R e fe r to H S o r
d is p a tc h s e r v ic e

6 67483-101
Diluent/Sheath Empty (CELL-DYN 3200)
DILUENT/SHEATH EMPTY (CELL-DYN 3200)

Diluent/Sheath Empty
Diluent/Sheath
(CELL-DYN Empty
3200)
(CELL-DYN 3200)
Press CLEAR FAULT and

open front left door

- cube empty?
- reagent correct?
Run background
Does error
Problem resolved No
recur?
Yes
Press CLEAR FAULT, run

background, and observe
diluent and sheath reservoirs
Does fluid reach the Check reservoirs

No
sensor electrodes? for cracks / leaks
Yes
Check sensor
contacts at top of
Refer to HS or
reservoirs are
dispatch service Cracks / leaks
No seated properly.
seen?
Reseat if
indicated. Run
background count.
Yes
Dispatch service
Does error
No Problem resolved
recur?
Yes
Page 1 of 2
Go to
A

67483-101 7
Diluent/Sheath Empty (CELL-DYN 3200)
DILUENT/SHEATH EMPTY (CELL-DYN 3200 )

A
Check for salt

buildup at
reservoir
connectors
and clean if
present
Does error
Problem resolved No
recur?
Yes
- Verify diluent/sheath plumbing is free

of restrictions
- Exercise valves 61, 62, 63, and 64
- Are reservoirs cracked and leaking?
- Clean sensor contacts (electrodes
and connector at top of reservoir) and
contacts are touching
Run background
Does error
Problem resolved No
recur?
Yes
Cycle power
Does error
Problem resolved No
recur?
Yes
Refer to HS or
dispatch service
Page 2 of 2

8 67483-101
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000)
REAGENT EMPTY - DILUENT, SHEATH, HGB LYSE (CELL-DYN 3000)

CELL-DYN 3000
CELL-DYN
Reagent Empty3000
Reagent
Diluent Empty
Diluent
Sheath
HGBSheath
Lyse
HGB Lyse
Clear fault
Press RUN and

observe reagent
reservoirs

- cube empty?
- reagent correct?
Does fluid
reach sensor No
- foam seal removed from under
contacts?
cap?
Yes - check fluid level in syringe
Clean sensor
Run background
contacts
Ensure sensor is
seated correctly
Does error
No Problem resolved
recur?
Does error
Problem resolved No
recur?
Yes
No
Yes
Check reservoir for
Does error cracks
Cycle power
recur?
Yes
Floss NCV tubing
Refer to HS or (V6-1 (Diluent), V6-2 (Sheath),
dispatch service V6-3 (HGB Lyse))
No Cracks seen?
for affected reservoir
or
replace check valve
Yes
Refer to HS or
Dispatch Service
Does error
Problem resolved No
recur?
Yes
Page 1 of 2 Go to
A

67483-101 9
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000)
REAGENT EMPTY - DILUENT, SHEATH, HGB LYSE (CELL-DYN 3000)
Exercise vacuum and pressure solenoids for

affected reservoir
Diluent pressure and vacuum - V5-1, 5-2
Sheath pressure and vacuum - V5-3, 5-4
HGB Lyse pressure and vacuum - V5-5, 5-6
Does error
Problem resolved No
recur?
Yes
Cycle power
Does error
Problem resolved No
recur?
Yes
Page 2 of 2 Refer to HS or
Dispatch Service

10 67483-101
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700)
REAGENT EMPTY - DILUENT, SHEATH, DETERGENT

(CELL-DYN 3500/3700)
CELL-DYN 3500/3700
CELL-DYN 3500/3700
Reagent Empty
Reagent
Diluent Empty
Diluent
Sheath
Sheath
Detergent
Detergent
Clear fault
Press RUN and

observe reagent
reservoirs

- cube empty?
- reagent correct?
Does fluid reach
No - vent hole in cap is open?
sensor contacts?
Yes - check fluid level in syringe
Clean sensor
contacts
Ensure sensor is
seated correctly
Does error
No Problem resolved
recur?
Does error
Problem resolved No
recur?
Yes
No
Yes
Check reservoir for
Does error
Cycle power cracks
recur?
Yes
Floss NCV tubing for

Refer to HS or affected reservoir
dispatch service No Cracks seen?
or
replace check valve
Yes
Refer to HS or
Does error Dispatch Service
Problem resolved No
recur?
Yes
Go to
Page 1 of 2 A

67483-101 11
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700)
REAGENT EMPTY - DILUENT, SHEATH, DETERGENT

(CELL-DYN 3500/3700)
A
Exercise vacuum and pressure solenoids for

affected reservoir
V5-1 Diluent pressure
V5-2 Diluent vacuum
V5-3 Sheath pressure
V5-4 Sheath vacuum
V5-5 Detergent pressure
V5-6 Detergent vacuum
Does error
Problem resolved No
recur?
Yes
Cycle power
Does error
Problem resolved No
recur?
Yes
Refer to HS or
Page 2 of 2 Dispatch Service

12 67483-101
Shear Valve Position Fault
SHEAR VALVE POSITION FAULT
S h e a r V a lv e
P o sSitio
h ena rF V
aua lt
lv e
P o s itio n F a u lt
D id e rro r o c c u r Is s h e a r v a lv e
C le a n a n d in s p e c t R e fe r to H S o r
a fte r c le a n in g s h e a r No d a m a g e d /p in s Yes
s h e a r v a lv e D is p a tc h s e rv ic e
v a lv e ? b ro k e n ?
Yes No
R e m o v e a n d re in s ta ll
R e in s ta ll s h e a r
s h e a r v a lv e c o rre c tly
v a lv e c o rre c tly
(C E L L -D Y N 3 0 0 0 :
u s e lu b ric a n t)
R e in itia liz e s y s te m
D o e s e rro r
No P ro b le m re s o lv e d
re c u r?
Yes
C y c le p o w e r
D o e s e rro r
No P ro b le m re s o lv e d
re c u r?
Yes
R e fe r to H S o r
d is p a tc h s e rv ic e

67483-101 13
Vacuum Accumulator Wet
VACUUM ACCUMULATOR WET
Vacuum
Vacuum
Accumulator W et
Accumulator W et
Observe waste
line for
obstructions or
correct draining
Is waste line Remove

draining No obstruction/reseat
correctly? tubing
Yes
Drain accumulators
CELL-DYN 3000: Follow Operator's Manual for

manual procedure
CELL-DYN 3500/3700/3200: through
DIAGNOSTICS menu
(go to Diagnostics, More, Drain accumulator)
Reinitialize system
Does error
No Problem resolved
recur?
Yes
Cycle power
Does error
No Problem resolved
recur?
Yes
Refer to HS or
dispatch service

14 67483-101
Clog Error (RBC or WIC)
CLOG ERROR (RBC OR WIC)
C log E rror (R B C or W IC )
C log E rror (R B C or W IC )
R un background
D oes error
P roblem resolved No
recur?
Y es P roblem resolved
C heck reagent cube and inlet lines: No

- cube em pty?
- reagent correct?
- inlet line reaches to bottom ?
D oes error
recur?
- foam seal rem oved from under cap?
- lines not crim ped?
Y es
R B C C log:
- clean/replace 10 m L syringe
- clean air filter
D oes error - run autoclean
recur?
V erify aperture plate(s) are correctly
installed and check for cracks
R eseat and clean per O P S m anual
Y es
E xercise solenoids around affected

transducers and m assage and reseat
tubing at solenoids
(S ee N ote)
NOTE:
S olenoids D oes error
C E LL-D Y N 3000: No P roblem resolved
recur?
3-1, 3-2, 3-3, 3-6, 3-7, 3-8
C E LL-D Y N 3500/3700:
R B C : 3-1, 3-2, 3-3, 3-4, 3-5, 3-7, 3-8 Y es
W IC : 8-1, 8-2, 8-3, 8-4, 8-5, 8-7, 8-8
G o to
A
P age 1 of 2

67483-101 15
Clog Error (RBC or WIC)
CLOG ERROR (RBC OR WIC)
Check
transducer(s) and
metering tubes for
cracks or leaks
Check quality of
Cracks/leaks
meniscus in No
seen?
metering tube
Yes
Check fitting between top
of metering tube and V 3-
7 (RBC) or V 8-7 (W IC) Good
No Dispatch Service
meniscus?
Clean or flush metering
tube if needed
Yes
Does error
Problem resolved No Yes Cycle power
recur?
Does error Refer to HS or

Page 2 of 2 Problem resolved No Yes
recur? dispatch service

16 67483-101
Flow Error (RBC or WIC)
FLOW ERROR (RBC OR WIC)

Flow Error
(RBCFlow Error
or WIC)
(RBC or WIC)
Run background
Does error
Problem resolved No
recur?
Yes
Inspect transducer/vent solenoids and

pinch tubing
Massage and reseat tubing in

solenoids as needed
SEE NOTE
Does error
Problem resolved No
recur?
Yes
Verify aperture plate(s) are

correctly installed and not
cracked
Reseat/replace if indicated
Does error
Problem resolved No
recur?
Yes
Check
transducer(s) and
metering tubes for
cracks or leaks
NOTE:
Solenoids
CELL-DYN 3000:
3-1, 3-2, 3-3, 3-6, 3-7, 3-8
Cracks/leaks Go to
CELL-DYN 3500/3700: No
seen? A
RBC: 3-1, 3-2, 3-3, 3-4, 3-5, 3-7, 3-8
WIC: 8-1, 8-2, 8-3, 8-4, 8-5, 8-7, 8-8
Yes
Dispatch Service
Page 1 of 2

67483-101 17
Flow Error (RBC or WIC)
FLOW ERROR (RBC OR WIC)

A

- cube empty?
- reagent correct?
Run background
Does error
Problem resolved No
recur?
Yes Note:
To clean RBC or WIC metering tube:
Check quality of CELL-DYN 3000: V38 (RBC)
meniscus in CELL-DYN 3500/3200: V38 (RBC), V88 (WIC)
metering tube -- it
should look like an 1. Disconnect tubing above V38 or V88.
upside down U. 2. Connect 20 mL syringe filled with 20% bleach
to tubing.
3. Open V38 or V88 until syringe is half empty.
Check fitting between top of 4. Release solenoid and wait 5 minutes.
metering tube and V 3-7 5. Open V38 or V88 until syringe is empty and
(RBC) or V 8-7 (WIC)and Good release solenoid.
No 6. Fill syringe with HGB Lyse reagent and repeat
exercise valves if indicated meniscus?
Clean metering tubes step 5.
(See Note) 7. Run background.
Yes
Does error
Problem resolved No Yes Run autoclean
recur?
Does error
No Problem resolved
recur?
Yes
Cycle power
Refer to HS or Does error

Yes No Problem resolved
Dispatch Service recur?
Page 2 of 2

18 67483-101
WOC / WBC Flow Errors
WOC / WBC FLOW ERRORS
WOC/WBC Flow
WOC/WBC
Errors Flow
Errors

- cube empty?
- reagent correct?
Is error occurring - inlet line reaches to bottom?
on one sample No - vent hole in cap is open?
only? - foam seal removed from under cap?
Yes
Rerun sample
Does error
Yes recur? No
Does error
Problem resolved No Drain/Fill reagents Replace reagents
recur?
Yes
Does error
No Problem resolved
Verify Results by an recur?
alternate method
Yes
Verify syringes:
- seated correctly
- moving correctly
- bubble free
See NOTE
Clean or replace
Syringe OK? No
as needed
Yes
Does error
NOTE: No Problem resolved
recur?
Check the following syringes:
CELL-DYN 3000: WBC metering syringe
CELL-DYN 3200: Sample injection syringe
CELL-DYN 3500/3700: WOC metering sheath syringes Go to
Yes
A
Page 1 of 2

67483-101 19
WOC / WBC Flow Errors
WOC / WBC FLOW ERRORS

A
Check transfer
peristaltic pump tubing
for wear or holes
Replace as needed
SEE NOTE
Does error
No Problem resolved
recur?
Yes
Verify WOC/WBC mixing chamber

fills correctly, bubble mix is good
(Swirl mix for CELL-DYN 3200)
Check for pinched

Mixing tubing
No
chamber OK? Exercise bubble
mix solenoid 2-4
Yes
Check channel
offsets
Run background
(<2.0, N/A on
CELL-DYN 3200)
Offsets in Does error

Dispatch Service No No Problem resolved
specs? recur?
No
Yes Yes
Yes
Does error
Cycle power
Clean Shear Valve recur?
Yes
Does error Does error

Cycle power Yes No Problem resolved Dispatch Service
recur? recur?
No
NOTE:
Problem resolved CELL-DYN 3000: WBC transfer peripump tubing
CELL-DYN 3200: Sample transfer peripump tubing
Page 2 of 2 CELL-DYN 3500/3700: WOC transfer peripump tubing

20 67483-101
Elevated PLT Backgrounds / LRI
ELEVATED PLT BACKGROUNDS / LRI

Elevated PLT
Elevated/ PLT
backgrounds LRI
backgrounds / LRI
Check that ground wire is

attached, front covers on Repeat
background
Does problem
Problem resolved No
recur?
Yes
Run electrical Is it a CELL-

No Yes Empty/fill flow cell
background DYN 3200?
Is electrical
Go to Does problem
No background = No Problem resolved
C recur?
0?
Yes Yes
Check diluent/sheath Check diluent/sheath

reagent: reagent:
- correct list #? - correct list #?
- frozen? - frozen?
Replace if needed and prime Replace if needed and prime
twice after replacing. twice after replacing
Does problem
Problem resolved No
recur?
Yes
Run autoclean
Does problem Go to
Problem resolved No Yes
recur? A
Page 1 of 4

67483-101 21

A
C h e c k D ilu e n t (D ilu e n t/S h e a th

s y rin g e fo r C E L L -D Y N 3 2 0 0 )
s y rin g e fo r le a k s / b u b b le s /b u ild u p
C le a n o r re p la c e if in d ic a te d
R u n b a c k g ro u n d
D o e s p ro b le m
P ro b le m re s o lv e d No
re c u r?
Yes
C le a n R B C /P L T A p e rtu re P la te
and
(C E L L -D Y N 3 0 0 0 /3 5 0 0 /3 7 0 0 o n ly )
s h e a r v a lv e a s p re c a u tio n
D o e s p ro b le m
re c u r?
Yes
C E L L -D Y N 3 0 0 0 /3 2 0 0 : C h e c k /
re p la c e (if in d ic a te d ) d ilu e n t/
s h e a th filte r
D o e s p ro b le m
re c u r?
Yes
D ra in /fill b a th s a n d re s e rv o irs
(flo w c e ll o n C E L L -D Y N 3 2 0 0 )
D o e s p ro b le m G o to
P ro b le m re s o lv e d No Yes
re c u r? B
P age 2 of 4

22 67483-101

B
Perform prepare
for shipping
procedure
Run 3-5
backgrounds
Does problem
Problem resolved No
recur?
Yes
Clean or drain
vacuum
accumulators
Run background
Does problem
Problem resolved No
recur?
Yes
Clean fan filters
Run background NOTE:

Transducer cleaning
1. From Main menu, press:

Does problem Special Protocols, Empty Xducers
Problem resolved No Yes Clean waste line 2. Disconnect vent tubing at top of transducer (at top
recur?
right port of first transducer)
3. Connect 20 mL syringe filled with 20% bleach
solution to top of the port
4. Inject bleach solution until the left side of
transducer is half filled and reconnect tubing.
5. Allow bleach to soak for 5 minutes.
6. Press: Fill xducers Main menu Run
Does problem
Problem resolved No Run background 7. Run 5 backgrounds
recur?
Yes
Check transducer electrodes for

corrosion Does problem
Yes Cycle power
Clean transducer if indicated recur?
(to clean transducer, see note:)
No
Does problem Refer to HS or

recur? Dispatch Service
Page 3 of 4

67483-101 23

C
Check for power cords resting on

diluent or waste lines
Remove if found
Run background
Does problem
Problem resolved No
recur?
Yes
Check waste sensor dummy

plug (if used)
Reseat plug
Does problem
Problem resolved No
recur?
Yes
Clean fan filters, drain or clean vacuum

accumulators, and waste line
Run background
Does problem
Problem resolved No
recur?
Yes
Check environment:
- printer/other devices on or near CELL-DYN? (e.g.: centrifuge, air conditioning)
- flickering/fluorescent lights?
- humidity?
Problem resolved
- CELL-DYN connected to UPS?
Remove potential interferences

No
Does problem Does problem

Problem resolved No Yes Cycle power
recur? recur?
Yes
Refer to HS or
Dispatch Service
Page 4 of 4 Yes

24 67483-101
Controls Out of Range
CONTROLS OUT OF RANGE

Controls Out of
Controls
Range Out of
Range
- Verify QC means/limits
- Check storage/handling
- Condition of QC when
received
- Rerun controls
Does problem
No Problem resolved
recur?
- cube em pty?
Yes - reagent correct?
Are backgrounds Troubleshoot
No - lines not crimped?
within specs? backgrounds No - ports not plugged?
Yes
Does problem
occur in one mode
Run Fresh
only?
Controls
Does problem
No Problem resolved
Yes Yes recur?
Troubleshoot aspiration
Does problem system of affected system Yes
recur?
Check sample aspiration

through Shear Valve
No Does problem
recur?
Problem resolved No
Yes
Are sam ples Go to Incomplete
No
aspirated correctly? Aspiration Tree
Run precision
Yes
Troubleshooting Precision within Yes

No Run autoclean
im precision specs?
Yes
Verify mode to mode Does problem

No Problem resolved
calibration recur?
Recalibrate if needed
Yes
Does problem
Problem resolved No
recur? Go to OPEN
/ CLOSED
m ode
Page 1 of 10

67483-101 25
Controls Out of Range
CONTROLS OUT OF RANGE

Problem occuring
in OPEN or
CLOSED mode
Issue resolved
OPEN /
CLOSED
No
Were Controls Controls still Try a different Controls still

No Yes
rerun? out of range? box of controls out of range?
No Yes
Yes
Issue resolved
Were controls mixed by
hand, warmed to room
Check affected temperature for 15
Yes
parameters minutes, and stored
No inside refrigerator shelf?
No
Are multiple
parameters out of No Redo controls
range? mixed by hand
Yes Follow flow chart

for affected
Clean shear valve, clean Y-fitting parameter Controls within
(for CELL-DYN 3000/3500/3700) range?
or Y-valve (for CELL-DYN 3200)
and rerun Controls
WOC/
NOC WIC RBC HGB MCV PLT
WBC
Yes
Go to Go to Go to Go to Go to Go to Go to
A B C D E F G
Does problem
Problem resolved No
recur?
Yes
Issue resolved
Yes
Check syringes:
- seated/moving
properly?
- filling?
Clean/replace as
needed
Does problem
Problem resolved No
recur?
Page 2 of 10

26 67483-101
Controls Out of Range - WBC / WOC
CONTROLS OUT OF RANGE - WBC / WOC

A
Run precision
Verify Sample Injection Syringe

and/or WOC Metering Syringe
are not leaking, have no
bubbles, and are moving up/
Precision within
Rerun precision down properly No
specs?
(visually inspect)
- change Transfer Pump

Tubing Yes
Empty/fill WOC Issue resolved

Is precision
No flow cell and rerun
within range?
controls
Yes
Yes Clean WOC flow

Does problem cell or perform Controls in
recur? clean for ship and range?
Issue resolved rerun controls
Run normal
No
patient sample
Is placement of
Replace WOC
neutrophil population
No peripump tubing
correct on 0/10
and rerun controls
scattergram?
Yes
Check channel offsets

(CELL-DYN 3000/3500/3700 only)
Are channel
Dispatch service No
offsets < 2.00?
Yes
Verify correct operation of

syringes
(see NOTE)
Clean or replace as needed
NOTE:
Check the following syringes:
CELL-DYN 3000: WBC metering syringe & WOC sheath syringe
CELL-DYN 3200: Sample injection syringe
CELL-DYN 3500/3700: WOC metering syringe & WOC sheath Does problem
No Problem resolved
syringe recur?
Yes
Page 3 of 10
Go to
AA

67483-101 27
Controls Out of Range - WBC / WOC
CONTROLS OUT OF RANGE - WBC / WOC
AA
Check WBC/WOC mixing

chamber for proper
draining, filling & bubble
mix
Exercise mix chamber
solenoids
Is mixing chamber
Dispatch service No draining/filling
correctly?
Yes
Verify WBC/WOC calibration by performing

a calibration verification and calibrate
if indicated
Run controls
Is the WOC
Problem resolved No
within ranger?
Yes
Dispatch service
Page 4 of 10

28 67483-101
Controls Out of Range - NOC (CELL-DYN 3200 Only)
CONTROLS OUT OF RANGE - NOC (CELL-DYN 3200 ONLY)

B

- cube empty?
Run precision - reagent correct?
Verify sample injection syringe - foam seal removed from under cap?
or WOC metering syringe is not - lines not crimped?
leaking, has no bubbles, and is - ports not plugged?
moving up/down properly Is precision - check fluid level in syringe
Rerun precision No
(visually inspect) within specs?
- change Transfer Pump

Tubing
Yes
Are HGB Check solenoid 41

Is precision
No results No Massage and
within range?
affected? reseat tubing
Yes Yes
Clean shear valve Does problem

Issue resolved No Problem resolved
and HGB flow cell recur?
Yes
Does problem
Problem resolved No Check sample
recur?
injection syringe
Clean/replace as
Yes needed
Check HGB Lyse Syringe:
- seated/moving properly?
- filling?
Clean or replace as Does problem
needed No Problem resolved
recur?
Yes
Does problem
Problem resolved No
recur?
Verify NOC calibration by
performing a calibration
verification and
Yes calibrate if indicated
Rerun controls
Check staging
pathway from
shear valve to
HGB flow cell for
obstructions Does problem
No Problem resolved
recur?
Yes
Does problem
Problem resolved No
recur?
Refer to HS or
dispatch service
Yes
Refer to HS or
Page 5 of 10 dispatch service

67483-101 29
Controls Out of Range - WIC
CONTROLS OUT OF RANGE - WIC

C
Refer to HS or Check reagent cube and inlet lines:

dispatch service - cube empty?
Run Precision
- reagent correct?
No - inlet line reaches to bottom?
Troubleshoot imprecision - lines not crimped?
Is precision by cleaning WIC aperture Is precision - ports not plugged?
No - check fluid level in syringe
within range? plate within specs?
Redo precision
Yes
Rerun
Yes
controls
Is WIC within
Problem resolved Yes
range?
No
Verify transducer operation by Solenoids

emptying, filling, draining & bubble mix 8-1, 8-2, 8-3, 8-4,
Exercise transducer solenoids, See chart of 8-5, and 8-7
massage and reseat tubing solenoids
to exercise
Is transducer
Refer to HS or
No operating
dispatch service
correctly?
Yes
Check WIC/HGB Lyse and

Diluent syringes
Clean/replace as needed
Does problem
Problem resolved No
recur?
Yes
Verify WIC calibration by

performing a calibration
verification
Calibrate if indicated
Rerun controls

Page 6 of 10

30 67483-101
Controls Out of Range - RBC
CONTROLS OUT OF RANGE - RBC

D
Run precision
Clean shear valve (ensure

inserted correctly)
Replace sample peripump
Troubleshoot Is precision tubing
No (except CELL-DYN 3200)
Imprecision within specs?
Yes
Does problem
Clean RBC aperture plate No Problem resolved
recur?
(except CELL-DYN 3200)
Yes
Massage tubing through solenoid

Does problem
Problem resolved No 5-4 and verify RBC chamber is
recur?
draining, filling, and swirl mixing
properly
for CELL-DYN 3200
Yes
Verify transducer operation

Solenoids
Exercise transducer solenoids,
3-1, 3-2, 3-3, 3-4, Does problem
massage and reseat tubing No Problem resolved
3-5, and 3-7 recur?
Yes
Is transducer
Refer to HS or
No operating
dispatch service CELL-DYN 3200:
correctly?
check vacuum #2
(3.5" open,
5.0" closed)
Yes
CELL-DYN 3200
Verify RBC mixing chamber
for proper fill/drain; swirl mix
Vac #2 within Refer to HS or
No
specs? dispatch service
Check Diluent syringe

Clean/replace as needed Yes
Verify RBC calibration by

performing calibration
verification using calibrator
Does problem Calibrate if indicated
Problem resolved No
recur?
Refer to HS or Does problem

Page 7 of 10

67483-101 31
Controls Out of Range - HGB
CONTROLS OUT OF RANGE - HGB

E

- cube empty?
Issue resolved - reagent correct?
Run precision
Yes - vent hole in cap is open?
Troubleshoot HGB - ports not plugged?
imprecision by - check fluid level in syringe
Is precision Is precision
cleaning HGB No
within range? within specs?
Flow Cell and
rerun precision
Yes
Check HGB reference and HGB

voltage within specs and document
No
HGB reference
within specs?
No
HGB Reference
Clean HGB flow >2200 for CELL-DYN 3000/ Refer to HS or
No Yes
cell 3500/3700 dispatch service
>2050 for CELL-DYN 3200
Refer to HS or HGB reference Check HGB syringes

No
dispatch service within specs? Check:
CELL-DYN 3200 Solenoids 2-4
-- 2-8 and massage tubing
CELL-DYN 3500/3700
Solenoid 3-6
Clean/replace as needed
Yes Yes
Rerun controls

Problem resolved No No Problem resolved
recur? recur?
Yes
Verify HGB calibration by performing the

calibration verification

Page 8 of 10

32 67483-101
Controls Out of Range - MCV
CONTROLS OUT OF RANGE - MCV

F

- cube empty?
Run precision - reagent correct?
Troubleshoot Is precision - check fluid level in syringe
No
Imprecision within specs?
Yes
Clean RBC Aperture Plate and RBC / PLT

Transducer
(CELL-DYN 3000/3500/3700 only)
CELL-DYN 3200: verify RBC mixing chamber

is draining and swirl mixing properly
Replace Diluent / Sheath if indicated
Does problem
Problem resolved No
recur?
Yes
Check RER data

(CELL-DYN 3000/3500/3700 only)
25-35% for normal patient
Refer to HS or Is RER data

No
dispatch service within specs?
Yes
Verify MCV calibration by performing

calibration verification
Rerun controls

Page 9 of 10

67483-101 33
Controls Out of Range - PLT
CONTROLS OUT OF RANGE - PLT

G

- cube empty?
- reagent correct?
Run precision
Troubleshoot imprecision by
cleaning RBC aperture plate
Is precision
or replacing Diluent / Sheath No CELL-DYN 3200:
within specs?
filter (for CELL-DYN 3200) RBC / PLT
Rerun precision mix chamber; swirl
Yes
Rerun controls CELL-DYN 3200:

massage tubing
through solenoid 5-4

recur? recur?
Yes Yes
Verify transducer operation Verify PLT calibration

Exercise transducer solenoids, by performing
Is precision
No massage and reseat tubing calibration verification
within range?
(CELL-DYN 3000/3500/3700
only) Calibrate if indicated
Rerun controls
Yes
Yes Is transducer
No operating No Problem resolved
correctly?
Yes Yes
Issue resolved
Check Diluent Refer to HS or

syringe dispatch service
Clean/replace as
needed
Does problem
Problem resolved No
recur?
Page 10 of 10

34 67483-101
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)
WIC / WOC MISMATCH (CELL-DYN 3500/3700 ONLY)

W IC / W OC
Mism atch
(m ultiple sam ples)
Run controls in
open m ode
Are W IC and
No Is W IC correct? No Troubleshoot W IC
W OC correct?
Yes Yes
Go to
D
Troubleshoot Troubleshoot
closed m ode W OC
Go to
A
Run precision
Troubleshoot Precision within

No
im precision specs?
Yes
Check closed
m ode aspiration
See Incom plete

Aspiration tree
Yes
Does problem
Problem resolved No
recur?
Yes
Verify m ode to
m ode calibration
Calibrate if
indicated
Does problem
Problem resolved No
recur?
Page 1 of 6

67483-101 35
WIC / WOC MISMATCH
Run precision

- cube empty?
- reagent correct?
No Yes - vent hole in cap is open?
imprecision specs?
Does problem
Problem resolved No
recur?
Yes
Check W OC Sheath and metering syringes:

- filled/seated/ moving properly?
Clean/replace if needed
Does problem
Problem resolved No
recur?
Yes
Check sample
aspirations/shear
valve
Is sample
Go to Incomplete
No aspirating
Aspiration tree
correctly?
Yes
Go to
Page 2 of 6 B

36 67483-101
WIC / WOC MISMATCH

B
Check WOC
mixing chamber
Check solenoids
Does chamber
1-7, 2-3, 2-4
No drain/fill and bubble
Massage and
mix properly?
reseat tubing
Yes
Replace WOC
Does problem
Problem resolved No Yes peristaltic pump
recur?
tubing
Does problem
Problem resolved No
recur?
Yes
Check staging pathway from WOC

mixing chamber to flow cell:
Check solenoids 2-1, 2-2, 2-3
Massage and reseat tubing
Does problem
Problem resolved No
recur?
Yes

in solenoids 2-5, 2-7
Does problem Go to
Problem resolved No
recur? C
Page 3 of 6

67483-101 37
WIC / WOC MISMATCH
Check channel
offsets
Are channel
Dispatch service
offsets less No
or refer to HS
than 2.00?
Yes
Verify WOC
calibration
Recalibrate if
indicated
Does problem
Problem resolved No
recur?
Yes
Dispatch Service
Page 4 of 6

38 67483-101
WIC / WOC MISMATCH

D
Run Precision

- cube empty?
- reagent correct?
No Yes - vent hole in cap is open?
imprecision specs?
Does problem
Problem resolved No
recur?
Yes
Check WIC/HGB diluent and

lyse syringes:
- filled/seated/moving properly?
Does problem
Problem resolved No
recur?
Yes
Clean WIC
Aperture Plate
Does problem
Problem resolved No
recur?
Yes
Go to
E
Page 5 of 6

67483-101 39
WIC / WOC MISMATCH

E
Check W IC
transducer
Is left cham ber of

Check solenoids 8-3, 8-4 W IC transducer
No
Massage and reseat tubing draining/filling
properly?
Check tubing between top

of transducer and vent
Does problem
Problem resolved No Yes accum ulator for crim ps
recur?
Massage and reseat
tubing
Does problem
Problem resolved No
recur?
Yes
Check sam ple

aspiration/shear
valve
Is sam ple
Go to Incom plete
No aspirating
Aspiration tree
correctly?
Yes
Verify W IC
calibration
Recalibrate if
indicated
Does problem
Problem resolved No
recur?
Yes
Dispatch Service
Page 6 of 6

40 67483-101
H & H Mismatch
H & H MISMATCH
H & H Mismatch
H & H Mismatch
Run controls to
determine affected
parameters
Run precision
Is precision Troubleshoot
No
within specs? imprecision
Yes
Affected
parameter
MCV RBC HGB
Go to Go to Go to
A B C
Page 1 of 5

67483-101 41
H & H Mismatch
H & H MISMATCH
C heck reagent cube and inlet lines:
- cube em pty?
- reagent correct?
A - vent hole in cap is open?
- foam seal rem oved from under cap?
- lines not crim ped?
D oes problem
recur?
Y es
R un autoclean
D oes problem
recur?
Y es
C lean R B C /P LT aperture plate

(C E LL-D Y N 3000/3500/3700 only)
D oes problem
recur?
Y es
C lean fan filters
D oes problem
recur?
Y es
P erform C alibration verification

C alibrate if indicated
D oes problem
P roblem resolved No Y es D ispatch S ervice
recur?
P age 2 of 5

42 67483-101
H & H Mismatch
H & H MISMATCH
B

- cube empty?
- reagent correct?
Does problem
Problem resolved No
recur?
Yes
Run autoclean
Does problem
Problem resolved No
recur?
Yes
Check Diluent syringe:

- Moving/filling OK?
- Bubble free?
Clean/replace syringe
Perform
Calibration
Does problem
Problem resolved No verification
recur?
Calibrate if
indicated
Yes
Clean RBC/PLT aperture plate

(CELL-DYN 3000/3500/3700 only) Does problem
recur?
Yes
Does problem
Problem resolved No
recur?
Dispatch Service
Page 3 of 5

67483-101 43
H & H Mismatch
H & H MISMATCH
C

- cube empty?
- reagent correct?
Does problem
Problem resolved No
recur?
Yes
Run autoclean
Does problem
Problem resolved No
recur?
Yes
NOTE:
HGB References:
CELL-DYN 3000 1850-2250 Check HGB
CELL-DYN 3500 1850-2250 Reference
CELL-DYN 3700 1800-2300 See Note
CELL-DYN 3200 1800-2300
Clean HGB flow HGB Reference

No
cell within specs?
Yes
Refer to HS or HGB Reference

No Check HGB lyse
Dispatch Service within specs?
syringe:
- Moving/filling OK?
- Bubble free?
Yes
Yes Clean/replace if needed
Does problem
Problem resolved No
recur?
Does problem
No Problem resolved
recur?
Yes
Go to
Page 4 of 5 D

44 67483-101
H & H Mismatch
H & H MISMATCH
Exercise solenoids:
CELL-DYN 3000/3500/3700: V 1-3
CELL-DYN 3200: V2-4
Massage/reseat tubing
Does problem
Problem resolved No
recur?
Yes
CELL-DYN 3500/3700:
Exercise WIC transducer drain
solenoids V8-2 and V8-3
Does problem
Problem resolved No
recur?
Yes
Perform calibration
verification
Does problem
Problem resolved No
recur?
Yes
Dispatch Service
Page 5 of 5

67483-101 45
Incomplete Aspiration
INCOMPLETE ASPIRATION
Incomplete
Incomplete
Aspiration
Aspiration
(multiple samples)
(multiple samples)
Does error
Go to
occur in both No
A
modes?
Yes
CELL-DYN 3000/3500/3700 CELL-DYN 3200
Inspect/change
sample peristaltic Check vacuum #2
pump tubing
Does error Vac #2 within Refer to HS or

Issue resolved No No
recur? specs? dispatch service
Yes Yes
Check sample
Flush Y-valve
sensor
Refer to HS or LED on/off Does error

No No Problem resolved
Dispatch Service appropriately? recur?
Yes
Inspect/clean
shear valve and
Yes
flush Y-fitting with
10% bleach

recur? Dispatch Service
Page 1 of 2

46 67483-101
Incomplete Aspiration
INCOMPLETE ASPIRATION
A
Closed Mode Open Mode

only only
Perform Sam ple path cleaning and include: Perform Sam ple path cleaning and include:
CELL-DYN 3000/3500/3700 : clean Y-fitting CELL-DYN 3000/3500/3700 : clean Y-fitting
CELL-DYN 3200: clean Y-valve CELL-DYN 3200: clean Y-valve
Does error Does error

recur? recur?
Yes
Yes
Check and exercise solenoids:
CELL-DYN 3000/3500/3700: 9-5 (open
Check/clean m ode)
sam ple/vent CELL-DYN 3200: Exercise V1-7 and verify
needles no restrictions between Y-valve and V1-7
Massage/reseat tubing
Does error
No Problem resolved
Does error recur?
Problem resolved No
recur?
Yes
Yes
Replace aspiration
peripum p tubing
Check SL LED (for CELL-DYN
3000/3500/3700
only)
Refer to HS or Sensor LED on/off

Run a Sam ple
No appropriately?
Dispatch Service
Yes Does error

No Issue resolved
occur?
Exercise
CELL-DYN 3000/3500/3700: solenoid 9-6
CELL-DYN 3200: exercise V1-2 and verify no
restrictions between Y-valve and V1-2 m assage/ Yes
reseat tubing Refer to HS or
Run another
dispatch service
Sam ple
Yes
recur? Dispatch Service Does LED sensor to
Does error
right of shear valve Yes
recur?
turns on/off?
No
No
Refer to HS or
Issue resolved
dispatch service
Page 2 of 2

67483-101 47
Increased RRBC Flags
INCREASED RRBC FLAGS

Increased RRBC
Increased
Flags RRBC
Flags

- cube empty?
- reagent correct?
Does problem
Problem resolved No
recur?
Yes
Clean Shear Valve
Does problem
Problem resolved No
recur?
Yes
Replace W OC
peristaltic pump
tubing
Does problem
Problem resolved No
recur?
Yes
Check W OC sheath and metering syringes

(on CELL-DYN 3200, it is the sample injection
syringe):
- seated and moving properly?
- bubble free?
Does problem
Problem resolved No
recur?
Yes
Go to
Page 1 of 3 A

48 67483-101

A
Check WOC mixing chamber

(on CELL-DYN 3500 and 3700 only) or clean it
if indicated - see WOC Chamber Cleaning Procedure page
60
(on CELL-DYN 3200: check WBC mixing chamber)
For CELL-DYN 3000/3500/3700: Check solenoids Does chamber drain/fill and

1-7, 2-3, 2-4 and massage and reseat tubing bubble mix properly?
No
For CELL-DYN 3200: Exercise solenoids 53, 55, 92 (on CELL-DYN 3200: does it
and massage and reseat tubing swirl mix and drain properly?)
Yes
Check staging pathway from
WOC mixing chamber to flow
Does problem cell:
recur?
Massage and reseat tubing in
staging solenoids
Does problem
Problem resolved No
recur?
Yes
Check channel
offsets
(CELL-DYN 3000/
3500/3700 only)
Are channel
offsets less
than 2.00?
Yes
Check 0/10
scattergram
Are lymph/neut
populations
No Dispatch Service
- correctly positioned?
- well defined?
Yes
Go to
B
Page 2 of 3

67483-101 49
Verify WOC
calibration
Recalibrate if
indicated
Does problem
Problem resolved No
recur?
Yes
Dispatch Service
Page 3 of 3

50 67483-101
Background Data Unacceptable
BACKGROUND DATA UNACCEPTABLE

Background Data
Background Data
Unacceptable
Unacceptable
Check that ground wire is

attached, front covers on.
Repeat background
Repeat
Problem resolved Yes background
OK?
No
Run electrical Is it a CELL-

No Yes Empty/fill flow cell
background DYN 3200?
Check for Is electrical

Does problem
electrical No background No Problem resolved
recur?
interference* OK?
Yes
Yes
Replace milipore
Does problem filter
Clean Aperture
recur?
Plates
No
Does problem
No Problem resolved
recur/
Yes Does problem
Problem resolved No
recur?
Yes
Dispatch Service Clean Transducers *Examples of electrical interference:

NOTE: See Elevated PLT - reagent lines near electrical wires
Backgrounds/LRI flow chart for Yes - printer on top of CELL-DYN
Transducer cleaning instructions - electrical appliances near CELL-
DYN
- flickering fluorescent lights
Does problem Go to
recur? A
Page 1 of 2

67483-101 51
Background Data Unacceptable
BACKGROUND DATA UNACCEPTABLE

- cube empty?
- reagent correct?
- inlet line reaches to bottom? Repeat
Replace reagent
- vent hole in cap is open? background
and prime twice
- foam seal removed from under cap? OK?
- check fluid level inNo
syringe
Yes
Repeat
Clean vacuum accumulator
background Yes Problem resolved
Clean fan filters
OK?
No
Perform prepare
for ship
Repeat
Problem resolved Yes background No Cycle power
OK?
Yes Repeat
background
OK?
No
Dispatch Service
Page 2 of 2

52 67483-101
Data Not Transmitting
DATA NOT TRANSMITTING

Data not
Data not
transmitting
transmitting
Start
First time to
Has it worked
No be hooked-up
before?
to an LIS?
Yes
No Using current rev Send current

No
of interface spec? interface spec
Yes
Yes Does problem

No Problem resolved
recur?
Yes
Check computer set-up

(see NOTE)
Cable connected to proper port
Is set-up
No Correct set-up
correct?
Does problem
recur?
Yes
NOTE:
Perform serial loop Go to
Refer to Operator's Manual for
back test A
computer setup
Page 1 of 2

67483-101 53
Data Not Transmitting
DATA NOT TRANSMITTING
Pass? Failed?
Yes Yes
Dispatch Service
Cycle power and
reseat LIS cable
Does problem
No Problem resolved
recur?
Yes
Refer to LIS
vendor
Page 2 of 2

54 67483-101
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700
RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500

AND 3700
Reticulocyte
Reticulocyte
Troubleshooting for
Troubleshooting
CELL-DYN 3200, for
CELL-DYN
3500 and 37003200,
3500 and 3700
Verify that stain has been stored in the dark at room

temperature, that Reticulocyte Backgrounds <100 and
exact dilution and incubation instructions have been
followed.
Are calibrated pipettes used?
Problems with
Go to
Both patients or QC out Patients Only
A
of range or both?
Is it sample Go to
Rerun QC QC Only No
specific? A
Yes
Still out of
Issue resolved No Rerun
range?
Yes
Is issue
No Issue resolved
Prepare a new dilution recurring?
of QC and rerun
Yes
Prepare a new dilution

of same sample and
Still out of
Issue resolved No rerun
range?
Yes
Is issue
No Issue resolved
Try new vials of recurring?
QC, prepare a new
dilution and rerun
Yes
Redraw sample or verify by

alternate method
Still out of
Issue resolved No
range?
Yes
Verify pipette is calibrated Yes Is issue

and proper technique followed recurring?
No
Is issue Go to
Issue resolved No Yes
recurring? A Issue resolved
Page 1 of 4

67483-101 55

AND 3700
A
Is background on
the stain <100? No Rerun background
Yes
Check maintenance
- extended autoclean - weekly
Is background
- clean metering syringe Yes
<100?
- clean sheath syringe
- drain and fill flow cell
No
Try new stain and

Maintenance Redo maintenance rerun
No
up to date? and rerun controls
No
Yes Controls within
range? Yes
Controls within
No
range? Yes
Do WOC gains and

Issue resolved
reticulocyte gains
match? Issue resolved
No
Yes
Follow procedure below:
1. For WOC gains: go to Main menu, Diagnostics, F12,
F1, Set point entry (0, 10, 90), and print screen
2. For reticulocyte gains: go to Reticulocyte mode,
Diagnostics, F12, F1, WOC (0, 10, 90)
3. Compare WOC gains and reticulocyte gains (0, 10,
90)
Do WOC and
Rerun QC Yes reticulocyte gains
match?
No
Go to Go to
B C
Page 2 of 4

56 67483-101

AND 3700
B C
Copy WOC gains as follows:

Enter WOC values (0, 10, 90) into
reticulocyte gains screen and
press Set analyzer to save entry
Rerun patient QC within

Yes Rerun QC
samples range?
No
Patient
Issue resolved Yes Run 3 -- 5 normal patient
samples OK? Controls within
samples in regular patient No
range? Yes
mode
No
Issue resolved
Verify by alternate Is neutrophil population

method at 0/10 scattergram at
Yes Rerun QC
the 2.5 by 2.5 hash
marks?
No
Check channel offsets are

Dispatch Service <2.0 Controls within
No
(only for CELL-DYN 3500 range? Yes
and 3700)
Issue resolved
Go to
D
Page 3 of 4

67483-101 57

AND 3700
D
Channel offsets within

Controls within range?
Issue resolved Yes Rerun controls Yes
range? (only for CELL-DYN 3500
and 3700)
No
Drain/fill flow cell, run autoclean, clean

WOC mixing chamber
(see WOC mixing chamber cleaning
No
procedure) and recheck channel
offsets
(only for CELL-DYN 3500 and 3700)
Controls within
Issue resolved Yes Rerun QC
range?
No Dispatch Service
References:
1. CELL-DYN 3500 Operator's Manual, June 1999: Chapter 14, 14-1 to 14-8
2. CELL-DYN 3700 Operator's Manual, November 2000: Chapter 14, 14-1 to 14-87
3. CELL-DYN 3200 Operator's Manual, October 2001: Chapter 14
Page 4 of 4

58 67483-101
WOC Flow Cell Cleaning Procedure (CELL-DYN 3000/3500/3700)
WOC FLOW CELL CLEANING PROCEDURE

(CELL-DYN 3000/3500/3700)
Materials/Tools Needed: 25% Bleach solution

10 mL syringe
Step Action
1 Fill syringe with 25% bleach solution.
2 From the Main Menu, press
SPECIAL PROTOCOLS
DISABLE/ENABLE ANALYZER
3 Insert bleach into WBC/WOC mixing chamber.

NOTE: Follow the steps below to avoid damaging the plastic port of
mixing chamber on CELL-DYN 3500/3700.
a. Follow tubing from mixing chamber to Overflow Chamber.
CELL-DYN 3000: top port of WBC mixing chamber
CELL-DYN 3500/3700: left top port of WOC mixing chamber
b. Disconnect tubing from overflow chamber.
c. Connect syringe to tubing.
d. Inject bleach solution and fill WBC/WOC mixing chamber 2/3 full.
e. Pull back on syringe slightly to remove any excess pressure and liquid
trapped in tubing.
f. Remove syringe and reconnect tubing to overflow chamber.
4 For Special Protocols Menu, press
ENABLE ANALYZER
5 Perform 2 autocleans with water to allow bleach to enter and clean flow cell.
6 Run 3 background counts.
7 Verify background counts are within specifications.

67483-101 59
WOC Flow Cell Cleaning Procedure (CELL-DYN 3200)
WOC FLOW CELL CLEANING PROCEDURE

(CELL-DYN 3200)
Materials/Tools Needed: 25% Bleach solution

10 mL syringe with tubing attached
Step Action
1 Fill syringe with 25% bleach solution.
2 From the Main Menu, press
SPECIAL PROTOCOLS
DISABLE/ENABLE ANALYZER
3 Insert bleach into WBC/WOC mixing chamber.

a. Remove tubing from mixing chamber (WBC or RBC/PLT).
b. Connect syringe and attach tubing to mixing chamber.
c. Inject bleach solution and fill WBC mixing chamber 2/3 full.
d. Pull back on syringe slightly to remove any excess pressure and liquid
trapped in tubing.
e. Remove syringe and reconnect tubing to overflow chamber.
4 For Special Protocols Menu, press
ENABLE ANALYZER
5 Perform 2 autocleans with water to allow bleach to enter and clean flow cell.
6 Run 3 background counts.
7 Verify background counts are within specifications.

60 67483-101

Celldyn Troubles

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CELL-DYN 3000/3500/3700/3200

Revision History Pages Revised and Added

CELL-DYN is a registered trademark of Abbott Laboratories.

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

RBC DILUENT SYRINGE OVERPRESSURE

Remove and reinstall

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

HGB LYSE EMPTY (CELL-DYN 3200)

Refer to HS or Cycle power

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

WBC LYSE EMPTY (CELL-DYN 3200)

Check reagent cube and inlet lines: Dispatch Service

Disable analyzer, and

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

LYSE EMPTY (CELL-DYN 3500/3700)

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

DILUENT/SHEATH EMPTY (CELL-DYN 3200)

Press CLEAR FAULT and

Check reagent cube and inlet lines:

Press CLEAR FAULT, run

Does fluid reach the Check reservoirs

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

DILUENT/SHEATH EMPTY (CELL-DYN 3200 )

Check for salt

- Verify diluent/sheath plumbing is free

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

REAGENT EMPTY - DILUENT, SHEATH, HGB LYSE (CELL-DYN 3000)

Press RUN and

Check reagent cube and inlet lines:

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

REAGENT EMPTY - DILUENT, SHEATH, HGB LYSE (CELL-DYN 3000)

Exercise vacuum and pressure solenoids for

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

REAGENT EMPTY - DILUENT, SHEATH, DETERGENT

Press RUN and

Check reagent cube and inlet lines:

Floss NCV tubing for

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

REAGENT EMPTY - DILUENT, SHEATH, DETERGENT

Exercise vacuum and pressure solenoids for

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

SHEAR VALVE POSITION FAULT

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

VACUUM ACCUMULATOR WET

Is waste line Remove

CELL-DYN 3000: Follow Operator's Manual for

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

CLOG ERROR (RBC OR WIC)

C heck reagent cube and inlet lines: No

E xercise solenoids around affected

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

CLOG ERROR (RBC OR WIC)

Does error Refer to HS or

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

FLOW ERROR (RBC OR WIC)

Inspect transducer/vent solenoids and

Massage and reseat tubing in

Verify aperture plate(s) are

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide

FLOW ERROR (RBC OR WIC)

Check reagent cube and inlet lines: