1LCI

FIREFLY LUCIFERASE

DOI: 10.2210 / pdb1lci / AP

Clasificacin: oxidorreductasa

Depositarn: 06/01/1996 Publicado: 26/03/1997

Autor (s) Deposicin: Conti, E. , francos,

NP , ladrillo, P.

Organismo: Photinus pyralis

Sistema de expresin Escherichia coli

Estructural de conocimientos de Biologa: 1LCI SBKB.org

>1LCI:A|PDBID|CHAIN|SEQUENCE
MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVV
CSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSV
VPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLS
KEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGEL
PAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL

ProtParam

User-provided sequence:
10 20 30 40 50 60
MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN ITYAEYFEMS

70 80 90 100 110 120

VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV AVAPANDIYN ERELLNSMNI

130 140 150 160 170 180

SQPTVVFVSK KGLQKILNVQ KKLPIIQKII IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD

190 200 210 220 230 240

FVPESFDRDK TIALIMNSSG STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV

250 260 270 280 290 300

VPFHHGFGMF TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL

310 320 330 340 350 360

IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL ITPEGDDKPG

370 380 390 400 410 420

AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG YVNNPEATNA LIDKDGWLHS

430 440 450 460 470 480

GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA PAELESILLQ HPNIFDAGVA GLPDDDAGEL

490 500 510 520 530 540

PAAVVVLEHG KTMTEKEIVD YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI

550
KAKKGGKSKL

References and documentation are available.

Number of amino acids: 550

Molecular weight: 60745.17

Theoretical pI: 6.42

Amino acid composition:

Ala (A) 42 7.6%

Arg (R) 20 3.6%
Asn (N) 19 3.5%
Asp (D) 31 5.6%
Cys (C) 4 0.7%
Gln (Q) 16 2.9%
Glu (E) 33 6.0%
Gly (G) 45 8.2%
His (H) 14 2.5%
Ile (I) 38 6.9%
Leu (L) 52 9.5%
Lys (K) 40 7.3%
Met (M) 14 2.5%
Phe (F) 30 5.5%
Pro (P) 29 5.3%
Ser (S) 29 5.3%
Thr (T) 29 5.3%
Trp (W) 2 0.4%
Tyr (Y) 19 3.5%
Val (V) 44 8.0%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%

(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 64

Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon C 2758
Hydrogen H 4337
Nitrogen N 715
Oxygen O 791
Sulfur S 18
Formula: C2758H4337N715O791S18
Total number of atoms: 8619

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 39560

Abs 0.1% (=1 g/l) 0.651, assuming all pairs of Cys residues form cystines

Ext. coefficient 39310

Abs 0.1% (=1 g/l) 0.647, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 35.06

This classifies the protein as stable.

Aliphatic index: 94.65

Grand average of hydropathicity (GRAVY): -0.040

Diseo del gen

ATGGAAGATGCGAAAAACATTAAAAAAGGCCCGGCGCCGTTTTATCCGCTGGAAGATGGC
ACCGCGGGCGAACAGCTGCATAAAGCGATGAAACGCTATGCGCTGGTGCCGGGCACCATT
GCGTTTACCGATGCGCATATTGAAGTGAACATTACCTATGCGGAATATTTTGAAATGAGC
GTGCGCCTGGCGGAAGCGATGAAACGCTATGGCCTGAACACCAACCATCGCATTGTGGTG
TGCAGCGAAAACAGCCTGCAGTTTTTTATGCCGGTGCTGGGCGCGCTGTTTATTGGCGTG
GCGGTGGCGCCGGCGAACGATATTTATAACGAACGCGAACTGCTGAACAGCATGAACATT
AGCCAGCCGACCGTGGTGTTTGTGAGCAAAAAAGGCCTGCAGAAAATTCTGAACGTGCAG
AAAAAACTGCCGATTATTCAGAAAATTATTATTATGGATAGCAAAACCGATTATCAGGGC
TTTCAGAGCATGTATACCTTTGTGACCAGCCATCTGCCGCCGGGCTTTAACGAATATGAT
TTTGTGCCGGAAAGCTTTGATCGCGATAAAACCATTGCGCTGATTATGAACAGCAGCGGC
AGCACCGGCCTGCCGAAAGGCGTGGCGCTGCCGCATCGCACCGCGTGCGTGCGCTTTAGC
CATGCGCGCGATCCGATTTTTGGCAACCAGATTATTCCGGATACCGCGATTCTGAGCGTG
GTGCCGTTTCATCATGGCTTTGGCATGTTTACCACCCTGGGCTATCTGATTTGCGGCTTT
CGCGTGGTGCTGATGTATCGCTTTGAAGAAGAACTGTTTCTGCGCAGCCTGCAGGATTAT
AAAATTCAGAGCGCGCTGCTGGTGCCGACCCTGTTTAGCTTTTTTGCGAAAAGCACCCTG
ATTGATAAATATGATCTGAGCAACCTGCATGAAATTGCGAGCGGCGGCGCGCCGCTGAGC
AAAGAAGTGGGCGAAGCGGTGGCGAAACGCTTTCATCTGCCGGGCATTCGCCAGGGCTAT
GGCCTGACCGAAACCACCAGCGCGATTCTGATTACCCCGGAAGGCGATGATAAACCGGGC
GCGGTGGGCAAAGTGGTGCCGTTTTTTGAAGCGAAAGTGGTGGATCTGGATACCGGCAAA
ACCCTGGGCGTGAACCAGCGCGGCGAACTGTGCGTGCGCGGCCCGATGATTATGAGCGGC
TATGTGAACAACCCGGAAGCGACCAACGCGCTGATTGATAAAGATGGCTGGCTGCATAGC
GGCGATATTGCGTATTGGGATGAAGATGAACATTTTTTTATTGTGGATCGCCTGAAAAGC
CTGATTAAATATAAAGGCTATCAGGTGGCGCCGGCGGAACTGGAAAGCATTCTGCTGCAG
CATCCGAACATTTTTGATGCGGGCGTGGCGGGCCTGCCGGATGATGATGCGGGCGAACTG
CCGGCGGCGGTGGTGGTGCTGGAACATGGCAAAACCATGACCGAAAAAGAAATTGTGGAT
 TATGTGGCGAGCCAGGTGACCACCGCGAAAAAACTGCGCGGCGGCGTGGTGTTTGTGGAT
GAAGTGCCGAAAGGCCTGACCGGCAAACTGGATGCGCGCAAAATTCGCGAAATTCTGATT
AAAGCGAAAAAAGGCGGCAAAAGCAAACTG

Expasy translate tool

5'3' Frame 1

Met E D A K N I K K G P A P F Y P L E D G T A G E Q L H K A Met K R Y A L V P G T I A F T D A H I E V N I
T Y A E Y F E Met S V R L A E A Met K R Y G L N T N H R I V V C S E N S L Q F F Met P V L G A L F I G V
A V A P A N D I Y N E R E L L N S Met N I S Q P T V V F V S K K G L Q K I L N V Q K K L P I I Q K I I
I Met D S K T D Y Q G F Q S Met Y T F V T S H L P P G F N E Y D F V P E S F D R D K T I A L I Met N S S
G S T G L P K G V A L P H R T A C V R F S H A R D P I F G N Q I I P D T A I L S V V P F H H G F G Met F T
T L G Y L I C G F R V V L Met Y R F E E E L F L R S L Q D Y K I Q S A L L V P T L F S F F A K S T L I D K Y
DLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
K P G A V G K V V P F F E A K V V D L D T G K T L G V N Q R G E L C V R G P Met I Met S G Y V N N P E
ATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
Q H P N I F D A G V A G L P D D D A G E L P A A V V V L E H G K T Met T E K E I V D Y V A S Q V T T A K
KLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL
5'3' Frame 2

W K Met R K T L K K A R R R F I R W K Met A P R A N S C I K R Stop N A Met R W C R A P L R L P Met R I

L K Stop T L P Met R N I L K Stop A C A W R K R Stop N A Met A StopT P T I A L W C A A K T A C S F L C
R C W A R C L L A W R W R R R T I F I T N A N C Stop T A Stop T L A S R P W C L Stop A K K A C R K
F Stop T C R K N C R L F R K L L L W I A K P I I R A F R A C I P L Stop P A I C R R A L T N Met I L C R K A
L I A I K P L R Stop L Stop T A A A A P A C R K A W R C R I A P R A C A L A Met R A I R F L A T R L F R I
P R F Stop A W C R F I Met A L A C L P P W A I Stop F A A F A W C Stop C I A L K K N C F C A A C R I I K
F R A R C W C R P C L A F L R K A P Stop L I N Met I Stop A T C Met K L R A A A R R Stop A K K W A K R
W R N A F I C R A F A R A Met A Stop P K P P A R F Stop L P R K A Met I N R A R W A K W C R F L K R K
W W I W I P A K P W A Stop T S A A N C A C A A R Stop L Stop A A Met Stop T T R K R P T R Stop L I
K Met A G C I A A I L R I G Met K Met N I F L L W I A Stop K A Stop L N I K A I R W R R R N W K A F C C S
I R T F L Met R A W R A C R Met Met Met R A N C R R R W W C W N Met A K P Stop P K K K L W I Met W
R A R Stop P P R K N C A A A W C L W Met K C R K A Stop P A N W Met R A K F A K F Stop L K R K K A
AKAN
5'3' Frame 3

G R C E K H Stop K R P G A V L S A G R W H R G R T A A Stop S D E T L C A G A G H H C V Y R C A
Y Stop S E H Y L C G I F Stop N E R A P G G S D E T L W P E H Q P S H C G V Q R K Q P A V F Y A G A
G R A V Y W R G G G A G E R Y L Stop R T R T A E Q H E H Stop P A D R G V C E Q K R P A E N S E R A
E K T A D Y S E N Y Y Y G Stop Q N R L S G L S E H V Y L C D Q P S A A G L Stop R I Stop F C A G K
L Stop S R Stop N H C A D Y E Q Q R Q H R P A E R R G A A A S H R V R A L Stop P C A R S D F W Q P
D Y S G Y R D S E R G A V S S W L W H V Y H P G L S D L R L S R G A D V S L Stop R R T V S A Q P A G
L Stop N S E R A A G A D P V Stop L F C E K H P D Stop Stop I Stop S E Q P A Stop N C E R R R A A E Q
R S G R S G G E T L S S A G H S P G L W P D R N H Q R D S D Y P G R R Stop Stop T G R G G Q S G A
V F Stop S E S G G S G Y R Q N P G R E P A R R T V R A R P D D Y E R L C E Q P G S D Q R A
D Stop Stop R W L A A Stop R R Y C V L G Stop R Stop T F F Y C G S P E K P D Stop I Stop R L S G G A
G G T G K H S A A A S E H F Stop C G R G G P A G Stop Stop C G R T A G G G G G A G T W Q N H D R
K R N C G L C G E P G D H R E K T A R R R G V C G Stop S A E R P D R Q T G C A Q N S R N S D Stop S
EKRRQKQT
3'5' Frame 1

QFAFAAFFRFNQNFANFARIQFAGQAFRHFIHKHHAAAQFFRGGHLARHIIH
N F F F G H G F A Met F Q H H H R R R Q F A R I I I R Q A R H A R I K N V R Met L Q Q N A F Q F R R R H
L I A F I F N Q A F Q A I H N K K Met F I F I P I R N I A A Met Q P A I F I N Q R V G R F R V V H I A A H N H
RAAHAQFAALVHAQGFAGIQIHHFRFKKRHHFAHRARFIIAFRGNQNRAGGF
G Q A I A L A N A R Q Met K A F R H R F A H F F A Q R R A A A R N F Met Q V A Q I I F I N Q G A F R K K
AKQGRHQQRALNFIILQAAQKQFFFKAIHQHHAKAANQIAQGGKHAK
A Met Met K R H H A Q N R G I R N N L V A K N R I A R Met A K A H A R G A Met R Q R H A F R Q A G A A
A A V H N Q R N G F I A I K A F R H K I I F V K A R R Q Met A G H K G I H A L K A L I I G F A I H N N N F L
NNRQFFLHVQNFLQAFFAHKHHGRLANVHAVQQFAFVINIVRRRHRHANKQ
R A Q H R H K K L Q A V F A A H H N A Met V G V Q A I A F H R F R Q A H A H F K I F R I G N V H F
N Met R I G K R N G A R H Q R I A F H R F Met Q L F A R G A I F Q R I K R R R A F F N V F R I F H
3'5' Frame 2

S L L L P P F F A L I R I S R I L R A S S L P V R P F G T S S T N T T P P R S F F A V V T W L A T Stop S T I
S F S V Met V L P C S S T T T A A G S S P A S S S G R P A T P A S K Met F G C C S R Met L S S S A G A
T Stop Stop P L Y L I R L F R R S T I K K C S S S S Q Y A I S P L C S Q P S L S I S A L V A S G L F
T Stop P L I I I G P R T H S S P R W F T P R V L P V S R S T T F A S K N G T T L P T A P G L S S P S G V I
R I A L V V S V R P Stop P W R Met P G R Stop K R F A T A S P T S L L S G A P P L A I S C R L L R S Y L
S I R V L F A K K L N R V G T S S A L Stop I L Stop S C R L R R N S S S S K R Y I S T T R K P Q I
R Stop P R V V N Met P K P Stop Stop N G T T L R I A V S G I I W L P K I G S R A W L K R T H A V R C G
S A T P F G R P V L P L L F I I S A Met V L S R S K L S G T K S Y S L K P G G R W L V T K V
Y Met L Stop K P Stop Stop S V L L S I I I I F Stop I I G S F F C T F R I F C R P F L L T N T T V G W
L Met F Met L F S S S R S L Stop I S F A G A T A T P I N S A P S T G I K N C R L F S L H T T Met R W L V
F R P Stop R F I A S A R R T L I S K Y S A Stop V Met F T S I C A S V N A Met V P G T S A Stop R F I A L
C S C S P A V P S S S G Stop N G A G P F L Met F F A S S
3'5' Frame 3

V C F C R L F S L Stop S E F R E F C A H P V C R S G L S A L H P Q T P R R R A V F S R W S P G S P
HNPQFLFRSWFCHVPAPPPPPAVRPHHHPAGPPRPHQKCSDAAAECFPV
P P A P P D S L Y I Stop S G F S G D P Q Stop K N V H L H P N T Q Y R R Y A A S H L Y Q S A R W S
L P G C S H S R S Stop S S G R A R T V R R A G S R P G F C R Y P D P P L S L Q K T A P L C P P R P
V Y H R L P G Stop S E S R W W F R S G H S P G E C P A D E S V S P P L R P L L C S A A R R R S Q
F H A G C S D H I Y Q S G C F S Q K S Stop T G S A P A A R S E F Y N P A G C A E T V L L Q S D T S
A P R E S R K S D S P G W Stop T C Q S H D E T A P R S E S R Y P E Stop S G C Q K S D R A H
G Stop S A R T R C D A A A P R L S A G R C C R C C S Stop S A Q W F Y R D Q S F P A Q N H I
R Stop S P A A D G W S Q R Y T C S E S P D N R F C Y P Stop Stop Stop F S E Stop S A V F S A R S
E F S A G L F C S Q T P R S A G Stop C S C C S A V R V R Y K Y R S P A P P P R Q Stop T A R P A P
A Stop K T A G C F R C T P Q C D G W C S G H S V S S L P P G A R S F Q N I P H R Stop C S L Q Y A
H R Stop T Q W C P A P A H S V S S L Y A A V R P R C H L P A D K T A P G L F Stop C F S H L P