Bos taurus toll-like receptor 2 (TLR2), mRNA

ggcaggtggc aggcagctac ggacggacag tcagcgcacc acaaagggac caccacagga

ctgaaatgtt taaactccgt ctcatcctca tctgctcttc aggcaaaatt ctgctgcgtt
ggtttggata gtgattgatg agttattcta atgcaggaga ctgtaaactt gagagtggag
gtcaaatcac tggacaatgc cacgtgcttt gtggacagca tgggtctggg ctgtcatcat
cctgtccacg gaaggagcct ctgaccaggc ttcttctctg tcttgtgacc caactggtgt
ctgcgatggc cattccagat ctttaaactc catcccctct ggtctcacgg caggtgtgaa
aagccttgac ctgtccaaca atgatatcac ctatgtcggc aacagagacc tgcagaggtg
tgtgaacctg aagactctga ggctgggggc caatgaaatt cacacagtgg aggaagattc
tttttttcac ctgaggaatc ttgaatattt ggacttatcc tataatcgct tatctaactt
atcatcctcc tggttcaggt ccctttatgt cttgaaattc ttaaacttac tgggaaattt
atacaaaaca cttggggaaa catctctttt ttctcatctc ccaaatctgc ggaccctaaa
agtaggaaat agtaacagct tcactgagat tcatgaaaag gatttcactg gactgacttt
tcttgaggag cttgagatca gtgctcaaaa tctgcagata tatgtgccaa agagtttaaa
gtcaatccag aacattagcc atctgattct acatctgaag cagcctattt tactcgtgga
cattcttgta gatattgtaa gttccttaga ttgttttgaa ctgagagata ctaatttgca
cactttccat ttttcagaag catccatcag tgaaatgagt acatcggtta aaaagcttat
atttagaaat gtgcaattca ccgatgaaag ttttgttgaa gttgtcaaac tgtttaacta
tgtttctggg atcttagaag tagagtttga tgactgtacc catgatggaa ttggcgattt
tagagcactg agtttggaca gaattagaca cctaggtaat gtggagacgt taacaatacg
gaagttgcat attccacagt ttttcttatt tcatgatctg agtagtatat atccactcac
aggcagagtt aaaagagtca caatagaaaa cagtaaggtt tttctggttc cttgtttact
ttcacaacat ttaaaatcat tagaatattt ggatctcagt gaaaacttaa tgtctgaaga
aaccttgaaa aactcagcct gtaaggatgc ctggcccttc cttcaaacct tggttttaag
gcagaatcgt ttgaaatcac tagaaaaaac gggagaactt ttgcttactc tggaaaatct
gaataacctt gatatcagta agaataattt tctttcaatg cctgaaactt gtcagtggcc
aggaaaaatg aaacagctga acttatccag cacaaggata cacagtttaa cccagtgcct
tccccagacc ctggaaattt tagatgttag caataacaat ctcgattcat tttctttgat
tttgccacaa ctcaaagaac tttatatttc cagaaataag ttgaagactc taccagatgc
ctccttctta cccgtgttat cagttatgag aattagcaga aatataataa atactttctc
aaaggaacaa cttgattctt ttcagcaact gaagacgttg gaggccggtg gcaacaactt
catttgctcc tgtgacttcc tgtccttcac acagggacag caggcactgg gccgtgtcct
ggtcgactgg cccgatgact accgctgtga ctctccctcc catgtgcggg gccagcgggt
gcaggacgcc cggctctccc tttctgaatg ccacagggcg gccgtggtgt ccgcagcgtg
ctgtgccctc ttcctgttgc tcctgctcac gggggtgctg tgtcaccgtt tccacggact
gtggtacatg aagatgatgt gggcgtggct gcaggccaag aggaagccca ggaaggctcc
ccgcagggac atctgctacg acgccttcgt gtcctacagc gagcgggatt cctactgggt
ggagaacctc atggtccagg agctggagca cttcaaccct ccctttaagc tgtgtcttca
taagcgagac ttcattcctg gcaagtggat tatcgacaac atcattgact ccattgaaaa
gagccacaaa accatctttg tgctttcgga gaactttgtg aagagcgagt ggtgcaagta
tgagctggac ttctcccatt tccgtctctt tgatgagaac aatgatgctg ccattctgat
tctgctggag cccattgaca agaaggccat tccccagcgc ttctgtaagc tgcggaagat
catgaacacc aagacctacc tggagtggcc cgtggatgag actcagcagg aagggttttg
gttaaatttg agagctgcaa taaggtccta ggatccttca ttaagggtca gtcttggtct
ggtggtggtc cttctatcac tagttataac tgagtccatt ctgacttaat tatatgtaaa
ccatccagtg aatattcatt ggaagaactg atgctgaagc tccaatactt tggccacctg
atgtgaagag ctgactcact ggaaaagacc ctgatgctgg gaaagattga gggaaggagg
aggagaaggg gatgacagag gatgagatgg ttggatggca tcactgactc agtggacatg
 aatttgagca aactccagga gatagtgaag gacaggaaag cctggtgtgc tgcagtccat
gaggttgcaa agagttggaa tttagcgacc gaacaacaac catatataaa ctattgaatg
aggacctgct tactaaaatg actaaaactg ttcaaatttt gtctagagtg ctgttttatc
aaaactgtga ccaaattgga aaaacatggt tctaggcttt tatttatcct atgagacagt
catgatctca gtccattatt gatatgtgaa ttatctgtag caattggctt agttcattag
gaaacagcag aaatgaatag agtttaagtg actttccatc agctttctgc caggggactc
atccatgtct ggagagggtg ttctttgtcc cccagctgtc ccttctgtgt gtgactgctc
tcaggagcaa caagacaaaa tgttaaagtc cttcagccaa atgtattgtt ctcaggaaaa
aaaagtgctg ttttgtgtcc ccattaagct ttacaggata ctaaaatacc tagagggtat
attttaaaat gaactgtgtg cgtcttcctc agaatttgtt aagtttctcc agccacagtt
acttgagaga gtgtatataa cccaaggtgc agtaaattat gtatttctat ggaaatgaaa
ctgatgaaat aaatactcag ttttgaagaa ttt

Using 1-based sequence positions

OLIGO start len tm gc% any 3' seq
LEFT PRIMER 363 20 59.97 50.00 4.00 2.00 gccttgacctgtccaacaat
RIGHT PRIMER 561 20 60.05 55.00 5.00 1.00 gacctgaaccaggaggatga
SEQUENCE SIZE: 3513
INCLUDED REGION SIZE: 3513

PRODUCT SIZE: 199, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

Forward OK

Tidak menemukan kecocokan dengan primer Reverse

Reverse complement : tcatcctcctggttcaggtc

Bos taurus interleukin 1, alpha (IL1A), mRNA
gacgagggag ccagtcatct cattgttgct agctcggttc agcaaagaag tgaagatggc
caaagtccct gacctctttg aagacctgaa gaactgttac agtgaaaatg aagactacag
ttctgaaatt gaccacctct ctctcaatca gaagtccttc tatgatgcaa gctatgagcc
acttcgtgag gaccagatga ataagtttat gtccctggat acctcggaaa cctctaagac
atccaagctt agcttcaagg agaatgtggt gatggtggca gccagtggga agattctgaa
gaagagacgg ttgagtttaa atcagttcat caccgatgat gacctggaag ccattgccaa
taatacagaa gaagaaatca tcaagcccag atcagcacat tacagcttcc agagtaacgt
gaaatacaac tttatgagag tcatccacca ggaatgcatc ctgaacgacg ccctcaatca
aagtataatt cgagatatgt caggtccata cctgacggct actacattaa ataatctgga
ggaggcagtg aaatttgaca tggttgctta tgtatcagaa gaggattctc agcttcctgt
gactctaaga atctcaaaaa ctcaactgtt tgtgagtgct caaaatgaag acgaacccgt
cttgctaaag gagatgcctg agacacccaa aatcatcaaa gatgagacca acctcctctt
cttctgggaa aagcatggct ctatggacta cttcaaatca gttgcccatc caaagttgtt
tattgccaca aagcaagaaa aattggtgca catggcaagt gggccgccct cgatcactga
ctttcagata ttggaaaaat agccttgact gtgcactcta cttacttgta aagtggtgac
catccgtatg tactatgtac atgaaggagt cgagcccttc actgttagtc actcgctgag
catgtgctga gcttttgtaa ttctaaatga atgtttactc tctttgtaag agagaacaca
aagtccaaca ctaacatata atgttgcttg ttatttaaac aacaccctat actttgcaaa
ctaccaatca atttaattat tattctgcac aataatcttg ggaggactga ggctactatc
tgtggctaca aaaggttctt tccatattat agatgagtaa actaaggcat aagaatacta
atacccatga cagcagttgg aataagccgt ggacacacaa tttcattcca actgctcagc
ttctactttt aagccactga tggacccttt atcaaatact ataagtttct ggggtctcag
tttgctgctg ctgctaagtc acttcagtca tgtccaactc tgtgcgaccc catagacggc
agcccaccag gctccgcagt ccctgggatt ctccaggcaa gaacactgga gtgggttgcc
atttccttct ccaatgcatg aagtgaaaag tgaaagtgaa gttgctcagt catgtccgac
tcttagcgac cccatggact gcagcctacc aggctcctct gtccatggga ttttccaggc
aagagtactg gagtggggtg ccattgcctt ctcccggggt ctcagtttga ccatcttcaa
aatcagggta atgatgacta tagccctcct acctcaacag tattttatgc caatgagttc
atttaagtaa aatttttctt gaagctgagc ctcaagaaga atgcaaagca tgaaatgtta
ttttaagtta ttatttatat gcatatatat ttataagcat tatttctaag atattattat
tatttataac atattattat atttatggca attccttgca atgtgtgagt atgaccaggt
atcttcaata atagtagaca gtgttttcta ggctgagtaa gtccgaggta ctaacgcact
ttggttcaaa gtgccttttc cattgtcatg aacttctgta ttccagtacc tgggagccct
gtgattatga taataaattt atattaattg ccctgtt

Using 1-based sequence positions

OLIGO start len tm gc% any 3' seq
LEFT PRIMER 1314 20 59.93 55.00 4.00 3.00 gtctcagtttgctgctgctg
RIGHT PRIMER 1502 20 59.99 55.00 6.00 3.00 gagtcggacatgactgagca
SEQUENCE SIZE: 2017
INCLUDED REGION SIZE: 2017

PRODUCT SIZE: 189, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

Tidak menemukan kecocokan dengan primer forward

Reverse OK
Bos taurus Toll-like receptor 4 (TLR4) mRNA,
complete cds
atgatggcgc gtgcccgcct ggctgcggct ctgatcccag ccacggccat cctctcctgc
ctgagaaccg agagttggga cccttgcgta caggttgttc ctaacattag ttaccagtgc
atggagctga atctctacaa aatccccgac aacatcccca tatcaaccaa gatgctggac
ctgagcttta actacctgag acatttaggc agccataact tctccagctt cccagaactg
caagtgctgg atttatccag atgtgaaatt aagattattg aagacgacac atttcagggc
ctaaaccacc tctccacctt gatactgacg ggaaacccta tccagagttt agcctgggga
gccttttctg ggctatcaag tttacagaag ctggtggccg tggagacaaa cctagtctct
ctaaatgact tccccattgg acatctcaaa aacttgaaag agcttaatgt ggctcacaat
tttatccatt ccttcaagtt acctgaatat ttttctaacc tgcccaacct ggagcacttg
gatctttcta acaacaaaat ccaaaatatt tattatgaag atgtgaaagt tctacatcaa
atgcccctac tcaacctctc tttagatttg tccttgaacc ctttagactt tattgaacca
ggtaccttta aagaaattaa gctcaatgga ttgactctgc gaagtaattt taacagttca
catgtcatga aaacctgtat tcaaggtctg gctggtttaa aaaccaaccg gctggttttg
ggagaattta aaaatgaaag gaagttgcaa agatttgaca gatctttcct ggagggactg
tgcaacctga ccattgaaca attccggata gcgtacttgg acaaattctc aggggacgat
acagacttat ttaattgttt ggcaaatgtt tctgtgattt ctctgttgag tatatcttta
ggaagtctac aagcccttct taaagatttt agatggcaac acttagaaat tattaactgt
gactttgaca agtttcctgc attgaagctc agttctctca aaaagtttgt tttcacagac
aacaaagata taagcacttt tactgagttt cagctaccaa gccttcagta tctagatctc
aaaagaaatc acttgagttt caagggttgc tgttctcaca ctgattttgg gacaaccaac
ctgaagcatt tagatctgag cttcaatgat gtcattacct taggttcaaa cttcatgggc
ttagagcaac tagaacacct ggattttcag cattccactc tgaaacagat caatgctttt
tcagcattcc tatcactcag aaacctccgc taccttgata tttcttatac caacatccgc
attgtcttcc atggcatctt tactggctta gtcagtctgc aaaccttgaa aatggcaggc
aactcttttc agaacaactt gctccctgac atcttcacag aactgactaa cttaaccgtc
ttggacctct ctaagtgtca actggaacag gtagcccaga cagcatttca ctccctctct
agccttcagg tgctgaatat gagtcacaac aaactcttgt cactggatac atttctttat
gaaccactcc actcgctccg gatcctagac tgcagcttca accgtatcat ggcctctaag
gagcaagaac tacagaattt gccaaggagc ctcacttggc taaatcttac tcagaatgca
tttgcttgtg tttgtgaaca tcagagtttc ctgcagtggg tcaaggacca gaggcagctc
ttggtgggag ctgagcaaat gatgtgtgca gagcctttag atatggagga catgccagtg
cttagtttca ggaacgccac ttgtcagctg agcaagacga tcatcagtgt gtcggtggtc
actgtgctcc tggtgtctgt ggtaggggtc ctagtctaca agttctattt ccacctgatg
cttcttgctg gctgcaaaaa gtatggcagg ggcgagagca tctatgatgc ctttgtcatc
tactcgagcc aggatgaaga ctgggtgcgg aatgaactgg taaagaactt ggaggagggc
gtgcccccct ttcagctctg ccttcactac agggacttta ttcctggggt ggccatcgcc
gccaatatca tccaggaggg tttccacaaa agccgtaagg ttattgtcgt ggtgtcccag
cacttcatcc agagccgatg gtgtatcttc gagtatgaga ttgcccagac ctggcagttt
ctgagcagcc gtgctggcat catcttcatc gtcctgcaga agctggagaa gtctctcctg
cggcagcagg tggagctcta tcgccttctg agcaggaaca cctacctgga gtgggaggac
agtgtcctgg ggcggcatgt cttctggaga agactcagaa aagccttgct ggctggtaaa
ccccagagtc cagaaggaac agcagatgca gaaaccaacc cgcaagaagc gacaacctcc
acctga
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 1986 20 60.01 45.00 5.00 1.00 tgctggctgcaaaaagtatg
RIGHT PRIMER 2198 20 59.97 45.00 3.00 1.00 ttacggcttttgtggaaacc
SEQUENCE SIZE: 2526
INCLUDED REGION SIZE: 2526

PRODUCT SIZE: 213, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

OK
Bos taurus interleukin 6 (IL6), mRNA
ccaggaacga aagagagctc catctgccct ccaggaacag ctatgaactc ccgcttcaca
agcgccttca ctccattcgc tgtctccctg gggctgctcc tggtgatgac ttctgctttc
cctaccccgg gtcccctggg agaagatttc aaaaatgaca ccaccccagg cagactactt
ctgaccactc cagagaaaac cgaagctctc attaagcgca tggtcgacaa aatctctgca
atgagaaagg agatatgtga gaagaatgat gagtgtgaaa gcagcaagga gacactggca
gaaaataagc tgaatcttcc aaaaatggag gaaaaggacg gatgcttcca atctgggttc
aatcaggcga tttgcttgat cagaaccact gctggtcttc tggagtatca gatatacctg
gactacctcc agaacgagta tgagggaaat caggaaaatg tcagggattt gaggaaaaat
atcagaacac tgatccagat cctgaagcaa aagatcgcag atctaataac cactccagcc
acaaacactg acctgctgga gaagatgcag tcttcaaacg agtgggtaaa gaacgcaaag
attatcctca tcctgagaaa ccttgagaat ttcctgcagt tcagcctgag agctattcgg
atgaagtagc tggggctccc atgattgtgg tagttcctgg gcattccctc ctctggtcag
aaacctgtcc actgggcaca caacttatgt tgttctctat gaagaactaa aagtatgagc
gttaggacac tattttatct ttaatttatt gatatttaaa tatgtgattt tgagttaatt
tatatacatg ataagtattt atatttttat gaagtgccac ttgaaatatt ttatgtattt
ggtttgaaaa agtaacgtaa aaatggctat gtggcttgaa tgtccttatt gttttggaga
caaatcattt cttgaaatgt gtaggcttac ctcaaaaaat ttgctaactt atgcatattt
ttaaaggcat atttatattg tatttatata atgtttaggc tgtttttata acaataaact
tcttttttaa agaaaaaaaa aaaaaaaa

Using 1-based sequence positions

OLIGO start len tm gc% any 3' seq
LEFT PRIMER 566 20 60.02 50.00 5.00 2.00 tgcagtcttcaaacgagtgg
RIGHT PRIMER 747 20 60.15 50.00 5.00 3.00 taagttgtgtgcccagtgga
SEQUENCE SIZE: 1108
INCLUDED REGION SIZE: 1108

PRODUCT SIZE: 182, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

OK
Bos taurus interleukin 8 (IL8) mRNA, complete
cds
aacaagagcc agaagaaacc tgacaaaaag cctcttgttc aaatatgact tccaagctgg
ctgttgctct cttggcagct ttcctgctct ctgcagctct gtgtgaagct gcagttctgt
caagaatgag tacagaactt cgatgccaat gcataaaaac acattccaca cctttccacc
ccaaatttat caaagaattg agagttattg agagtgggcc acactgtgaa aattcagaaa
tcattgttaa gcttaccaat ggaaacgagg tctgcttaaa ccccaaggaa aagtgggtgc
agaaggttgt gcaggtattt gtgaagagag ctgagaagca agatccatga

Using 1-based sequence positions

OLIGO start len tm gc% any 3' seq
LEFT PRIMER 85 20 60.08 55.00 6.00 0.00 tgctctctgcagctctgtgt
RIGHT PRIMER 274 20 59.97 50.00 5.00 3.00 cagacctcgtttccattggt
SEQUENCE SIZE: 350
INCLUDED REGION SIZE: 350

PRODUCT SIZE: 190, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

OK
Bos taurus CXCR1 mRNA, complete cds
atgacaatca tcctgaaaga tttatctaat agcagttatt tgtgggaggg gtttgaggat
gagtttgaaa attacagcgg cacgccaccc acagaagact atgattatag tccccgtgag
ataagcactg agacactcaa caagtatgct gtggtcgtca tctatgccct ggtcttcttg
ctaagcctcc tgggaaactc cctggtgatg ctggtcatct tatacagccg gattggtcgc
tctgtcactg atgtctacct gctgaacctg gccatggctg acctgctctt cgccatgacc
ctgcctatct gggccgcctc caaggcaaag ggctggatct tcggcacacc cctgtgcaag
gtggcctcac tcctgaagga agtgaacttc tacagcggta ttctactgct ggcctgcatc
agcatggacc gctacctggc cattgtccat gccacacgca cgctgaccca gaagcggcac
tgggtcaagt tcatatgttt aggcatctgg gccctgtccg tgatcctggc cctgcccatc
ttcatcttcc gtgaggccta tcaaccacca tactccgacc tagtctgcta cgaggacctg
ggtgccaata caacgaaatg gcggatgata atgcgtgtcc taccccagac ctttggcttc
ctcctgcccc tgctggtcat gctgttctgc tacggattca ccctgcgcac gctgttttca
gcccaaatgg ggcagaagca ccgggccatg cgggtcatct ttgctgtcgt gctcgtcttc
ctgctctgct ggctgcccta caacctggtc ctgatcgcag acaccctcat gagggcccat
gtgattgctg agacctgtca gcgccgcaat gacattggcc gggccctgga tgccaccgag
atcctgggct tcctgcacag ctgcctcaac cccctcatct acgtcttcat tggccagaag
tttcgccacg gactcctcag gatcatggcc atccgtggcc tgatcagcaa ggagttcttg
gccaaggatg gcaggccctc ctttgttggc tcttcttcag ggaacacgtc tactaccctc
tga

No mispriming library specified

Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 755 20 60.14 50.00 2.00 2.00 tcatctttgctgtcgtgctc
RIGHT PRIMER 945 20 59.93 55.00 4.00 1.00 gacgtagatgagggggttga
SEQUENCE SIZE: 1083
INCLUDED REGION SIZE: 1083

PRODUCT SIZE: 191, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

Tidak menemukan kecocokan dengan primer Forward

Reverse OK