BAC-Tracker Supercoiled DNA Ladder

Cat. No. BT010950

The BAC-Tracker Supercoiled DNA Ladder is suitable for agarose gel size estimation
of large supercoiled DNAs, such as BAC (Bacterial Artificial Chromosome) clones. The
improved BAC-Tracker Ladder contains 7 (rather than 4) discreet supercoiled DNA bands
of 8, 28, 38, 55, 95, 120 and 165 kb in a ready-to-load solution. The additional 28 and 165 kb
bands improve size estimations and the 8 kb band facilitates screening for empty or
non-recombinant BAC clones.
The BAC-Tracker Ladder is formulated to provide approximately equal staining intensity
for all 7 bands. Sufficient DNA is provided for 50 gel lanes (500 l).
Applications
Size analysis of BAC clones by agarose minigel electrophoresis.
Size analysis of large supercoiled plasmid DNA or other extra-chromosomal DNAs
from any source.

Product Specifications
Storage: Store at room temperature when used daily. Store at 4C or 20C for long term
storage.
Usage: Load 10 l of BAC-Tracker Supercoiled DNA Ladder per gel lane. We recommend
using a 1% agarose gel in 1 X TAE Buffer at 4C at 4.5 V/cm for 2.5 hours.

Note: Cm in V/cm refers to the length of the electrophoresis apparatus not the length of the gel.
Following electrophoresis, stain the gel using 1 g/ml ethidium bromide or SYBR Gold
(per manufacturers standard protocol).

Note: Detection of low copy number clones (e.g. BAC clones) or plasmids may require
prolonged staining times.
Quality Control: Each batch of BAC-Tracker Supercoiled DNA Ladder is checked by
agarose gel electrophoresis and ethidium bromide staining.
Related Products: The following products are also available:
CopyControl BAC Cloning Kits
BACMAX DNA Purification Kit
Colony Fast-Screen Kit (Size-Screen)

www.epicentre.com Lit. # 173 6/2011 1

BAC-Tracker Supercoiled DNA Ladder

165 Kb
120 Kb
95 Kb

55 Kb

38 Kb

28 Kb

non-marker
DNA

8 Kb

10 l separated on a 1% agarose minigel.

Figure 1. The BAC-Tracker Supercoiled DNA Ladder is composed of supercoiled

DNAs of 8, 28, 38, 55, 95, 120, and 165 kb. The broad band of DNA that appears
below the 28 Kb band is non-marker DNA.

BACMAX, BAC-Tracker, CopyControl, and Fast-Screen are trademarks of Epicentre, Madison, Wisconsin.
SYBR is a registered trademark of Molecular Probes Inc., Eugene, Oregon.

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