BO CO KT QU THC TP HA SINH

NH LNG GLUCOSE TRONG HUYT THANH,

NH LNG AMYLASE HUYT, V PHN TCH
NC TIU
S nhm thc tp: 22
Ngy thc tp: 13/2/2017
Thnh vin:
1. Trn Vinh Quang
2. Bi Quang Minh
I. nh lng Glucose trong huyt thanh:
1. Nguyn tc:
Nng glucose huyt thanh c xc nh bng phn ng oxy ho vi enzyme
glucose oxidae (GOD). Phn ng to ra hydroperoxid (H2O2). Cht ny c ly gii bi
phenol v 4-aminophenazon di xc tc ca enzyme peroxidae (POD) to ra
quinoneimine c mu tm. Cng mu ca quinoneimine t l thun vi lng
glucose ban u c trong mu.
Phng trnh phn ng:
GOD
Glucose + O2 + H2O Gluconic acid + H2O2

POD
2H2O2 + 4-aminophenazon + phenol quinoneimine + 4H2O

2. Cng thc tnh nng glucose trong huyt thanh:

OD o
Glucose huyt thanh [mg/dL] = OD chun *100
3. Kt qu:

NG CHUN NG O
Huyt thanh 10l
Dd glucose chun 10l
Thuc th R 1000l 1000l
OD 0,12 0,118
Nng glucose (mg/dL) 98,33

4. Bin lun:
- Trong c th bnh thng, chuyn ho glucid lun c iu ho theo nhu cu ca c
th th hin quan trng v r rt nht l s iu ho ng huyt.

- Bnh thng ng huyt 70 120 mg/dL hay 4,22 6,67 mmol/L.

- Kt qu o c nng glucose trong huyt thanh l 98,33 mg/dL nn nm trong

ngng bnh thng.

- ng huyt lun n nh nh s cn bng gia hai ngun:

+ B sung, cung cp glucose vo mu (ngoi sinh v ni sinh).
+ S dng glucose cc t chc (m c, m m, )
+ Glucose c lin tc lc qua qun cu thn v c ti hp thu hon ton qua ng
thn.

II. nh lng Amylase huyt :

1. Nguyn tc:

Amylase thu phn 2-chloro-4-nitrophenyl-maltotrioside (CNPG3) thnh

2-chloro-4-nitrophenol (CNP), 2-Chloro-4Nitrophenyl-maltodioside v glucose (G) theo
phn ng sau:
5CNPG3 Amylase 3CNP + 2CNPG2 + 3G3 + 2G
S gii phng CNP t c cht v s tng hp th ca n t l thun vi hot
amylase huyt thanh.

2. Kt qu:

nh lng bilirubin ton phn:

 Cho vo ng 37oC
Mu th 10l
Thuc th RGT 1000l
Hot amylase (U/L) 132,2
3. Bin lun:

Tr s bnh thng:
Ngi ln: 53-123 U/L, ngi c tui tng nh hn so vi gi tr trn.

Hot amylase mu o c ca mu huyt thanh l 132,2 cao hn tr s bnh thng.

C th hot amylase mu tng do nhng nguyn nhn sau:

- Vim tu cp: do ru, t min.
- t tin trin cp ca vim tu mn.
- Vim tu cp do thuc.
- Tc nghn ng mt, bnh l ng mt, .

III. Tng phn tch nc tiu:

S dng giy th 10 thng s xc nh cc thng s sau (ng vi mu nc tiu sinh
vin):

1. pH: 7
2. T trng: 1,015
3. Nitrite: neg
4. Bch cu: 1+
5. Glucose: norm
6. Cetone: -
7. Protein: -
8. Urobilinogen: norm
9. Bilirubin: -
10. Mu: neg

Vi cc thng s trn mu nc tiu ny bnh thng.