Introduction to Chromatography

Chromatography is a separation technique based on the different interactions of

compounds with two phases, a mobile phase and a stationary phase, as the
compounds travel through a supporting medium.

mobile phase: a solvent that flows through the supporting medium

stationary phase: a layer or coating on the supporting medium that

interacts with the analytes

supporting medium: a solid surface on which the stationary phase is

bound or coated

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 What is Chromatography

1) Solvent Extraction :
transfer of a solute from phase 1 phase 2
S (in phase1) S (in phase 2)

Partition coefficient

K
s 2
s 1
 What is Chromatography

A solute equilibrates between a mobile and a stationary phase.

The more it interacts with the stationary phase, the slower it is moved along a
column.

Xm Xs
Xm
Ks =
Xs
Solutes with a large Ks value will be retained more strongly by the stationary
phase.
 Interaction Between Solutes, Stationary
Phase, and Mobile Phase

Differences in the interactions between the solutes and stationary and

mobile phases enable separation.

Solute
Degree of adsorption,
solubility, ionicity, etc.

Stationary
Mobile phase
phase
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The analytes interacting most
strongly with the stationary
phase will take longer to pass
through the system than those
with weaker interactions.

These interactions are usually

chemical in nature, but in some
cases physical interactions can
also be used.

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How do we describe a chromatogram

A graph showing the detectors response as a function of

elution time :
bands shapes, position, resolution.

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 Theory of Chromatography
1.) Typical response obtained by chromatography (i.e., a chromatogram):

chromatogram - concentration versus elution time

Wh

Wb

Inject

Where:
tR = retention time
tM = void time
Wb = baseline width of the peak in time units 9
Wh = half-height width of the peak in time units
Note: The separation of solutes in chromatography depends on two factors:
(a) a difference in the retention of solutes (i.e., a difference in their time
or volume of elution
(b) a sufficiently narrow width of the solute peaks (i.e, good efficiency for
the separation system)
Peak width & peak position
determine separation of peaks

A similar plot can be made in terms of elution volume instead of elution

time. If volumes are used, the volume of the mobile phase that it takes
to elute a peak off of the column is referred to as the retention volume
(VR) and the amount of mobile phase that it takes to elute a non-
retained component is referred to as the void volume (VM).

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2.) Solute Retention:

A solutes retention time or retention volume in chromatography is

directly related to the strength of the solutes interaction with the mobile
and stationary phases.

Retention on a given column pertain to the particulars of that system:

- size of the column
- flow rate of the mobile phase

Capacity factor (k): more universal measure of retention, determined

from tR or VR.
k = (tR tM)/tM

or
k = (VR VM)/VM
capacity factor is useful for comparing results obtained on different
systems since it is independent on column length and flow-rate. 11
The value of the capacity factor is useful in understanding the retention mechanisms for a
solute, since the fundamental definition of k is:
moles Astationary phase
k =
moles Amobile phase

k is directly related to the strength of the interaction between a solute with the stationary
and mobile phases.

Moles Astationary phase and moles Amobile phase represents the amount of solute present in each
phase at equilibrium.

Equilibrium is achieved or approached at the center of a chromatographic peak.

When k' is # 1.0, separation is poor

When k' is > 30, separation is slow
When k' is = 2-10, separation is optimum

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A simple example relating k to the interactions of a solute in a column is illustrated for
partition chromatography:

KD
A (mobile phase)
A (stationary phase)

where: KD = equilibrium constant for the distribution of A between the mobile

phase and stationary phase

Assuming local equilibrium at the center of the chromatographic peak:

[A]stationary phase Volumestationary phase

k =
[A]mobile phase Volumemobile phase

Volumestationary phase
k = KD
Volumemobile phase

As KD increases, interaction of the solute with the stationary phase becomes more
favorable and the solutes retention (k) increases 13
 Volumestationary phase
k = KD
Volumemobile phase

Separation between two solutes requires different KDs for their

interactions with the mobile and stationary phases

since DG = -RT ln KD
peak separation also represents different changes in free energy

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How do we describe a chromatogram

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How do we describe a chromatogram

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3.) Efficiency:

Efficiency is related experimentally to a solutes peak width.

- an efficient system will produce narrow peaks
- narrow peaks smaller difference in interactions in order to
separate two solutes

Efficiency is related theoretically to the various kinetic processes that

are involved in solute retention and transport in the column
- determine the width or standard deviation (s) of peaks

Wh Estimate s from peak widths,

assuming Gaussian shaped peak:

Wb = 4s

Wh = 2.354s

Dependent on the amount of time that a solute spends in the column (k or tR)

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Number of theoretical plates (N): compare efficiencies of a system for solutes that have
different retention times

N = (tR/s)2

or for a Gaussian shaped peak

N = 16 (tR/Wb)2

N = 5.54 (tR/Wh)2

The larger the value of N is for a column, the better the column will be able to separate
two compounds.
- the better the ability to resolve solutes that have small differences in retention
- N is independent of solute retention
- N is dependent on the length of the column

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Plate height or height equivalent of a theoretical plate (H or HETP): compare efficiencies of
columns with different lengths:

H = L/N

where: L = column length

N = number of theoretical plates for the column

Note: H simply gives the length of the column that corresponds to one theoretical plate

H can be also used to relate various chromatographic parameters (e.g., flow rate, particle size,
etc.) to the kinetic processes that give rise to peak broadening:

Why Do Bands Spread?

a. Eddy diffusion
b. Mobile phase mass transfer
c. Stagnant mobile phase mass transfer
d. Stationary phase mass transfer
e. Longitudinal diffusion

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a.) Eddy diffusion a process that leads to peak (band) broadening due to the presence
of multiple flow paths through a packed column.

As solute molecules travel through the column,

some arrive at the end sooner then others simply
due to the different path traveled around the
support particles in the column that result in
different travel distances.

Longer path arrives at end of column after (1).

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b.) Mobile phase mass transfer a process of peak broadening caused by the
presence of different flow profile within channels or
between particles of the support in the column.

A solute in the center of the channel

moves more quickly than solute at the
edges, it will tend to reach the end of
the channel first leading to band-
broadening

The degree of band-broadening due to eddy diffusion and mobile phase

mass transfer depends mainly on:

1) the size of the packing material

2) the diffusion rate of the solute

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c.) Stagnant mobile phase mass transfer band-broadening due to differences in the
rate of diffusion of the solute molecules between the
mobile phase outside the pores of the support
(flowing mobile phase) to the mobile phase within
the pores of the support (stagnant mobile phase).

Since a solute does not travel down

the column when it is in the stagnant
mobile phase, it spends a longer time
in the column than solute that
remains in the flowing mobile phase.

The degree of band-broadening due to stagnant mobile phase mass

transfer depends on:

1) the size, shape and pore structure of the packing material

2) the diffusion and retention of the solute
3) the flow-rate of the solute through the column 22
d.) Stationary phase mass transfer band-broadening due to the movement of solute
between the stagnant phase and the stationary phase.

Since different solute molecules

spend different lengths of time in the
stationary phase, they also spend
different amounts of time on the
column, giving rise to band-
broadening.

The degree of band-broadening due to stationary phase mass transfer

depends on:

1) the retention and diffusion of the solute

2) the flow-rate of the solute through the column
3) the kinetics of interaction between the solute and the
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stationary phase
e.) Longitudinal diffusion band-broadening due to the diffusion of the solute along the
length of the column in the flowing mobile phase.

The degree of band-broadening due

to longitudinal diffusion depends on:

1) the diffusion of the solute

2) the flow-rate of the solute through
the column

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Van Deemter equation: relates flow-rate or linear velocity to H:

H = A + B/m + Cm

where:

m = linear velocity (flow-rate x Vm/L)

H = total plate height of the column
A = constant representing eddy diffusion &
mobile phase mass transfer
B = constant representing longitudinal diffusion
C = constant representing stagnant mobile phase
& stationary phase mass transfer

One use of plate height (H) is to relate these kinetic process to band broadening to a
parameter of the chromatographic system (e.g., flow-rate).

This relationship is used to predict what the resulting effect would be of varying this
parameter on the overall efficiency of the chromatographic system.

Number of theoretical plates(N) (N) = 5.54 (tR/Wh)2 peak width (Wh)

H = L/N 25
Plot of van Deemter equation shows how H changes with the linear velocity (flow-rate) of
the mobile phase

m optimum

Optimum linear velocity (mopt) - where H has a minimum value and the point of maximum
column efficiency:

mopt = rB/C

mopt is easy to achieve for gas chromatography, but is usually too small for liquid
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chromatography requiring flow-rates higher than optimal to separate compounds
4.) Measures of Solute Separation:

separation factor (a) parameter used to describe how well two solutes are separated by
a chromatographic system:

a = k2/k1 k = (tR tM)/tM

where:
k1 = the capacity factor of the first solute
k2 = the capacity factor of the second solute,
with k2 $ k1

A value of a $1.1 is usually indicative of a good separation

Does not consider the effect of column efficiency or peak widths, only retention. 27
resolution (RS) resolution between two peaks is a second measure of how well two
peaks are separated:
tr2 tr1
RS =
(Wb2 + Wb1)/2
where:
tr1, Wb1 = retention time and baseline width for the
first eluting peak
tr2, Wb2 = retention time and baseline width for the
second eluting peak

Rs is preferred over a since both

retention (tr) and column efficiency
(Wb) are considered in defining
peak separation.

Rs $ 1.5 represents baseline

resolution, or complete separation
of two neighboring solutes ideal
case.

Rs $ 1.0 considered adequate for

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most separations.
 Types of Chromatography
1.) The primary division of chromatographic techniques is based on the type of mobile phase
used in the system:

Type of Chromatography Type of Mobile Phase

Gas chromatography (GC) gas
Liquid chromatograph (LC) liquid

2.) Further divisions can be made based on the type of stationary phase used in the system:

Gas Chromatography
Name of GC Method Type of Stationary Phase
Gas-solid chromatography solid, underivatized support
Gas-liquid chromatography liquid-coated support
Bonded-phase gas chromatography chemically-derivatized support

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 Types of Chromatography
Liquid Chromatography
Name of LC Method
Adsorption chromatography
Partition chromatography
Ion-exchange chromatography
Size exclusion chromatography
Affinity chromatography
Type of Stationary Phase
solid, underivatized support
liquid-coated or derivatized support
support containing fixed charges
porous support
support with immobilized ligand
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3.) Chromatographic techniques may also be classified based on the type of support material
used in the system:

Packed bed (column) chromatography

Open tubular (capillary) chromatography
Open bed (planar) chromatography

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 Mobile Phase: Liquid

Stationary Phase Separation Mechanism

- Solid Adsorption
- Liquid Layer Partition
- Ion exchange resin Ion exchange
- Microporous beads Size Exclusion
- Chemically modified resin Affinity

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 Column Chromatography and Planar
Chromatography

Separation column

Paper or a
substrate coated
with particles

Packing material

Column Chromatography Paper Chromatography

Thin Layer Chromatography (TLC)33
 PARTITION CHROMATOGRAPHY

Partition chromatography can be subdivided into

(i) liquid-liquid chromatography and
(ii) bonded-phase chromatography.

With liquid-liquid, a liquid stationary phase is retained on

the surface of the packing by physical adsorption.

With bonded-phase, the stationary phase is bonded

chemically to the support surfaces.

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 PARTITION CHROMATOGRAPHY
Early partition chromatography was the liquid-liquid type;
now the bonded-phase method has become predominate
because of certain disadvantages of liquid-liquid systems.

One of these disadvantages is the loss of stationary

phase by dissolution in the mobile phase, which requires
periodic recoating of the support particles.

Furthermore, stationary-phase solubility problems prohibit

the use of liquid-phase packings for gradient elution.

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 Columns for Bonded-Phase Chromatography
The supports for the majority of bonded-phase
packings for partition chromatography are
prepared from rigid silica, or silica-based,
compositions.

These solids are formed as uniform, porous,

mechanically sturdy particles commonly having
diameters of 3, 5, or 10mm. The surface of fully
hydrolyzed silica is made up of chemically reactive
silanol groups.

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The most useful bonded-phase coatings are
siloxanes formed by reaction of the hydrolyzed
surface with an organochlorosilane.

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 Reversed-Phase and Normal-Phase Packings
Two types of partition chromatography are
distinguishable based upon the relative polarities of
the mobile and stationary phases.

Liquid chromatography based upon highly polar

stationary phases such as water or triethyleneglycol
supported on silica or alumina particles; a relatively
nonpolar solvent such as hexane or I-propylether
then served as the mobile phase. This type of
chromatography is referred to as normal-phase
chromatography.
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 Reversed-phase chromatography
The stationary phase is nonpolar, often a
hydrocarbon, and the mobile phase is relatively
polar (such as water, methanol, or acetonitrile).

In normal-phase chromatography, the least polar

component is eluted first because it is the most
soluble in the mobile phase; increasing the polarity
of the mobile phase has the effect of decreasing
the elution time.

In contrast, in the reversed-phase method, the most

polar component appears first, and increasing the
mobile phase polarity increases the elution time. 40
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 Ion Chromatography

Separation of inorganic cations and anions

and low molecular weight water-soluble
organic acids and bases

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 Ion Exclusion Chromatography

Separation of ionic solutes from weakly

ionized of neutral solutes using strong
cation and anion exchange resins

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 Factors Affecting Retention in IC
Degree of ionization of the solute
- solute pKa
- eluent pH
- organic modifier content
hydrophobic interactions between solute
and sp
molecular size of the solute
degree of X-linkage of the sp
system temperature 44
 Affinity Chromatography

Separation of proteins and biomolecules

through the use of a biologically specific
immobilized ligand (sp).
Biologically specific ligand is chemically
attached to a gel matrix
pH of the system is controlled to control
the strength of the interaction between
the ligand and the solute molecule
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 Complexation Chromatography

Separation based on the rapid

reversible formation of a complex
between metal ions and ligands
Suitable ligands are immobilized on sp

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 Chiral Chromatography

Separation based on the use of chiral

stationary phases which interact to
varing degrees with optical isomers of
the solute
Systems differ little from conventional
HPLC systems
Limited applications for a given sp
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GAS CHROMATOGRAPHY

Mobile phase is a gas!

Stationary phase could be anything
but a gas

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 Gas Chromatography (GC)

GC is currently one of the most popular methods for separating and analyzing
compounds.

This is due to its high resolution, low limits of detection, speed, accuracy and
reproducibility.

GC can be applied to the separation of any compound that is either naturally volatile (i.e.,
readily goes into the gas phase) or can be converted to a volatile derivative.

This makes GC useful in the separation of a number of small organic and inorganic
compounds (They can be big compounds if you can make them small before separation!)

A simple GC system consists of:

1. Gas source (with pressure and flow regulators)

2. Injector or sample application system (sample inlet)
3. Chromatographic column (with oven for temperature control)
4. Detector & computer or recorder
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Carrier gas: He (common), N2, H2
Pinlet 10-50 psig
Flow = 25-150 mL/min packed column
Flow = 1-25 mL/min open tubular column
Column: 2-100 m coiled stainless steel/glass/Teflon/fused silica, packed vs. unpacked
Oven: 0-400 C ~ average boiling point of sample
Accurate to <1 C 54
Detectors: FID, TCD, ECD, NPD, FPD, AED, PID, MSD. (SINGLE OR TANDEM)
55
(a) Filling sampling loop
(b) Introduction of sample into column
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 Mobile Phase
GC separates solutes based on their different interactions between mobile and stationary
phases.

solutes retention is determined mostly by its vapor pressure and volatility

solutes retention is controlled by its interaction with the stationary phase

Carrier gas main purpose of the gas in GC is to move the solutes along the column, mobile
phase is often referred to as carrier gas (MUST BE INERT!).

Common carrier gas: include He, Ar, H2, N2

Carrier Gas or Mobile phase does not affect solute retention, but does affect:

1.) Desired efficiency for the GC System (Van Deemter!)

- low molecular weight gases (He, H2) larger diffusion coefficients
- low molecular weight gases faster, more efficient separations
2.) Stability of column and solutes
- H2 or O2 can react with functional groups on solutes and stationary
phase or with surfaces of the injector, connections and detector
3.) Response of the detector
- thermal conductivity detector requires H2 or He 57
- other detectors require specific carrier gas compatibility
 Mobile Phases
Stationary phase in GC is the main factor determining the selectivity and retention
of solutes.

There are three types of stationary phases used in GC:

Solid adsorbents
Liquids coated on solid supports
Bonded-phase supports

1.) Gas-solid chromatography (GSC)

- same material is used as both the stationary phase and support material
- common adsorbents include:
alumina
molecular sieves (crystalline aluminosilicates [zeolites] and clay)
silica
active carbon

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Magnified Pores in activated carbon
 Gas-Solid Chromatography
Advantages:

- long column lifetimes

- ability to retain and separate some compounds not easily resolved by other
GC methods

geometrical isomers
permanent gases

Disadvantages:

- very strong retention of low volatility or polar solutes

- catalytic changes that can occur on GSC supports

- GSC supports have a range of chemical and physical environments

different strength retention sites

non-symmetrical peaks
variable retention times
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 Gas-Liquid Chromatography
2.) Gas-liquid chromatography (GLC) LIKE DISSOLVES LIKE!

- stationary phase is a liquid coated on a solid support

- over 400 liquid stationary phases available for GLC
- material range from polymers (polysiloxanes, polyesters, polyethylene glycols) to
fluorocarbons, and liquid crystals etc.

Based on polarity, of the 400 phases available only 6-12 are needed for most separations.
The routinely recommended phases are listed below:

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