Terazosin

Molecular formula: C19H25N5O4

Molecular weight: 387.4
CAS Registry No.: 65390-64-7 (terazosin),
70024-40-7 (terazosin hydrochloride dihydrate),
63074-08-8 (terazosin hydrochloride)

SAMPLE
Matrix: blood, dialysate, urine
Sample preparation: 200 |xL Serum, plasma, urine, or dialysate + 400 |xL 200 ng/mL
prazosin hydrochloride in MeCN at 5, mix, centrifuge at 5 for 5-10 min. Remove the
supernatant and evaporate it to dryness at 40-50 under a stream of air. Dissolve residue
in 400 |xL 100 mM mobile phase, inject a 50-100 |xL aliquot.

HPLCVARIABLES
Column: 300 X 3.9 5 jim Spherisorb ODS
Mobile phase: MeCN:THF:4-20 mM pH 5 sodium phosphate 25:6:69
Flow rate: 1.6
Injection volume: 50-100
Detector: F ex 250 em 370 (filter)
CHROMATOGRAM
Retention time: 6
Internal standard: prazosin (9)
Limit of quantitation: 5 ng/mL (blood, dialysate), 50 ng/mL (urine)

KEYWORDS
serum; plasma; human; dog
REFERENCE
Patterson, S.E. Rapid and sensitive analysis of terazosin in plasma, peritoneal dialysis solution, and
urine using high-performance liquid chromatography with fluorescence detection. J.Chromatogr.,
1984, 311, 206-212

SAMPLE
Matrix: blood, dialysate, urine
Sample preparation: 1 mL Plasma, 1 mL dialysate, or 200 |xL urine + 100 |xL 1 M NaOH,
mix, add 5 mL ethyl acetate: benzene 20:80 containing 2-50 ng/mL prazosin hydrochloride
(Caution! Benzene is a carcinogen!), vortex for 5 min, centrifuge. Remove 4-4.5 mL of the
organic phase and evaporate it to dryness at 40-50 under a stream of air. Dissolve residue
in 300 uX 100 mM phosphoric acid or mobile phase, inject a 15-100 |xL aliquot.

HPLCVARIABLES
Column: 150 X 3.9 5 ^m IBM C 1
Mobile phase: MeCN:THF:4-20 mM pH 7.0 sodium phosphate 22:6:72
Flow rate: 1
Injection volume: 15-100
Detector: F ex 250 em 370 (filter)
CHROMATOGRAM
Retention time: 5
Internal standard: prazosin (8)
Limit of quantitation: 1 ng/mL (blood, dialysate), 10 ng/mL (urine)
KEYWORDS
plasma; human; dog

REFERENCE
Patterson, S.E. Rapid and sensitive analysis of terazosin in plasma, peritoneal dialysis solution, and
urine using high-performance liquid chromatography with fluorescence detection. J.Chromatogr.,
1984, 311, 206-212

SAMPLE
Matrix: blood, urine
Sample preparation: 50 JULL Plasma or urine + 100 jxL saturated NaCl + 50 JJLL 2 /xg/mL
dimethothiazine mesylate in water + 50 \xL 4 M NaOH, vortex for 10 s, add 200 JULL
MTBE, vortex for 30 s, centrifuge at 9950 g for 2 min, inject a 110 \xh aliquot of the
organic phase.

HPLCVARIABLES
Column: 250 X 5 5 (Jim Spherisorb S5W
Mobile phase: MeOH: 10 mM ammonium perchlorate adjusted to pH 6.7 with 1 mL/L
methanolic NaOH (0.1 M)
Flow rate: 2
Injection volume: 110
Detector: F ex 370 em 370-700

CHROMATOGRAM
Retention time: 4
Internal standard: dimethothiazine mesylate (5)
Limit of detection: 1 |xg/mL

OTHER SUBSTANCES
Extracted: prazosin
Simultaneous: N-acetylprocainamide, ajmaline, chlorpromazine, desipramine, dipyrida-
mole, doxazosin, flecainide, flurazepam, gallopamil, imipramine, ketanserin, metoprolol,
mexiletine, mianserin, nadolol, nitrazepam, orphenadrine, oxprenolol, penbutolol, pin-
dolol, prajmalium, procainamide, propranolol, protriptyline, pyrimethamine, quinidine,
quinine, triamterene, trimipramine, verapamil
Noninterfering: amiodarone, atenolol, disopyramide, labetalol, lignocaine, lorcainide,
methyldopa, nifedipine, prenalterol, propafenone, sotalol, timolol

KEYWORDS
plasma

REFERENCE
Bhamra, R.K.; Flanagan, R.J.; Holt, D.W. High-performance liquid chromatographic measurement of
prazosin and terazosin in biological fluids. J.Chromatogr., 1986, 380, 216-221

SAMPLE
Matrix: bulk
Sample preparation: Dissolve 100 mg bulk material in 100 mL MeCN: 50 mM pH 4.4
citrate buffer containing 10 mL 0.2 mg/mL 3,4-dimethoxybenzoic acid in MeOH, inject a
50 |xL aliquot.

HPLC VARIABLES
Column: 150 X 4.6 5 jim Zorbax Rx C8
Mobile phase: MeCN: isopropanol: 50 mM pH 4.4 citrate buffer 175:50:1775
Flow rate: 2
Injection volume: 50
Detector: UV 254

CHROMATOGRAM
Retention time: 27
Internal standard: 3,4-dimethoxybenzoic acid

OTHER SUBSTANCES
Simultaneous: impurities

KEYWORDS
rugged

REFERENCE
Bauer, J.F.; Krogh, S.K.; Chang, Z.L.; Wong, CF. Determination of minor impurities in terazosin hy-
drochloride by high-performance liquid chromatography. J.Chromatogr., 1993, 648, 175-181

SAMPLE
Matrix: solutions
Sample preparation: Prepare a 10 |xg/mL solution in MeOH, inject a 20 jxL aliquot.

HPLC VARIABLES
Column: 125 X 4.9 Spherisorb S5W silica
Mobile phase: MeOH containing 10 mM ammonium perchlorate and 1 mL/L 100 mM
NaOH in MeOH, pH 6.7
Flow rate: 2
Injection volume: 20
Detector: E, LeCarbone, V25 glassy carbon electrode, + 1.2 V

CHROMATOGRAM
Retention time: 1.8

OTHER SUBSTANCES
Also analyzed: acebutolol, acepromazine, acetophenazine, N-acetylprocainamide, albu-
terol, alprenolol, amethocaine, amiodarone, amitriptyline, antazoline, atenolol, azacy-
clonal, bamethane, benactyzine, benperidol, benzethidine, benzocaine, benzoctamine,
benzphetamine, benzquinamide, bromhexine, bromodiphenhydramine, bromperidol,
brompheniramine, brompromazine, buclizine, bufotenine, bupivacaine, buprenorphine,
butacaine, butethamate, chlorcyclizine, chlorpheniramine, chlorphenoxamine, chlorpren-
aline, chlorpromazine, chlorprothixene, cimetidine, cinchonidine, cinnarizine, clemastine,
clomipramine, clonidine, cocaine, cyclazocine, cyclizine, cyclopentamine, cyproheptadine,
deserpidine, desipramine, dextromoramide, dextropropoxyphene, dicyclomine, diethylcar-
bamazine, diethylpropion, diethylthiambutene, dihydroergotamine, dimethindene, di-
methothiazine, diphenhydramine, diphenoxylate, dipipanone, diprenorphine, dipyrida-
mole, disopyramide, dothiepin, doxapram, doxepin, doxylamine, droperidol, ephedrine,
ergocornine, ergocristine, ergocristinine, ergocryptine, ergometrine, ergosine, ergosinine,
ergotamine, ethopropazine, etorphine, etoxeridine, fenethazine, fenfluramine, fenoterol,
fentanyl, flavoxate, fluopromazine, flupenthixol, fluphenazine, fiurazepam, haloperidol,
hydroxyzine, hyoscine, ibogaine, imipramine, indapamine, iprindole, isothipendyl, isox-
suprine, ketanserin, laudanosine, lidocaine, lofepramine, loxapine, maprotiline, mecamy-
lamine, meclophenoxate, meclozine, medazepam, mephentermine, mepivacaine, mep-
tazinol, mepyramine, mesoridazine, metaraminol, methadone, methamphetamine,
methapyrilene, methdilazene, methotrimeprazine, methoxamine, methoxyphenamine,
methoxypromazine, methylephedrine, methylergonovine, methysergide, metoclopramide,
metopimazine, metoprolol, mianserin, morazone, nadolol, nalorphine, naloxone, napha-
zoline, nicotine, nifedipine, nomifensine, nortriptyline, noscapine, orphenadrine, oxeladin,
oxprenolol, oxymetazolin, papaverine, pargyline, pecazine, penbutolol, pentazocine, pen-
 thienate, pericyazine, perphenazine, phenadoxone, phenampromide, phenazocine, phen-
butrazate, phendimetrazine, phenelzine, phenglutarimide, phenindamine, pheniramine,
phenmetrazine, phenomorphan, phenoperidine, phenothiazine, phenoxybenzamine, phen-
tolamine, phenylephrine, phenyltoloxamine, physostigmine, piminodine, pimozide, pin-
dolol, pipamazine, pipazethate, piperacetazine, piperidolate, pipradol, pirenzepine, piri-
tramide, pizotifen, practolol, pramoxine, prazosin, prenylamine, prilocaine, primaquine,
proadifen, procainamide, procaine, proehlorperazine, procyclidine, proheptazine, prolin-
tane, promazine, promethazine, pronethalol, properidine, propiomazine, propranolol, pro-
thipendyl, protriptyline, proxymetacaine, pseudoephedrine, pyrimethamine, quinidine,
quinine, ranitidine, rescinnamine, sotalol, tacrine, terbutaline, terfenadine, thenyldi-
amine, theophylline, thiethylperazine, thiopropazate, thioproperazine, thioridazine, thi-
othixene, thonzylamine, timolol, tocainide, tolpropamine, tolycaine, tranylcypromine, tra-
zodone, trifluoperazine, trifluperidol, trimeperidine, trimeprazine, trimethobenzamide,
trimethoprim, trimipramine, tripelennamine, triprolidine, tryptamine, verapamil,
xylometazoline

REFERENCE
Jane, L; McKinnon, A.; Flanagan, R. J. High-performance liquid chromatographic analysis of basic drugs
on silica columns using non-aqueous ionic eluents. IL Application of UV, fluorescence and electro-
chemical oxidation detection. J.Chromatogr., 1985, 323, 191-225