University of Santo Tomas

Faculty of Pharmacy
Department of Biochemistry

HIGH PERFORMANCE
LIQUID
CHROMATOGRAPHY

GANDOLA | GO | GUTIERREZ | IMBAO | JAMORABON | LABASTIDA | 1C-Biochemisty

 PRINCIPLES
Cosmetics are considered one of the
advances today in Chemistry that
enhances physical attributes of a
person and is applied externally.
These are mixtures of chemical
compounds, some of these are
obtained from natural sources and
some being synthetic.
 STATEMENT OF THE PROBLEM
This study aims to answer the
following:
Whether there is lead in the
liquid samples?
If the presence of lead
can be tested by means
of using precipitating
reagents and
confirmatory tests
OBJECTIVE

Determine the presence of Lead

SIGNIFICANCE OF THE STUDY

Prove the presence of Lead in

locally sold lipstick samples

Raise awareness

Present data as caution

SCOPE
& DELIMITATION
4 lipstick samples
Lead
No specific amount
 DEFINITION OF TERMS
Lipstick

Lead

Precipitating Reagent

Precipitate

Confirmatory Test
MATERIALS
& METHODOLOGY
4 lipstick samples
Test tubes
Hot plate
Casseroles
1M Nitric acid (HNO3)
3M Hydrochloric acid (HCl)
Potassium iodide solution (KI).
METHODOLOGICAL
FRAMEWORK
Preparation of the Liquid
Lipstick Samples

Adding the precipitating

reagent to the Liquid Lipstick
Samples

Confirmatory Tests
RESULTS
& DISCUSSION
FIRST
TRIAL
 RESULTS &
DISCUSSION
SECOND
TRIAL

HNO3- dissolving agent

HCl- precipitating agent
KI- confirmatory
CONCLUSION

Lead is proven to be present on the

lipstick samples.
 RECOMMENDATION
The use of other lipstick
samples
The use of ICP-EOS