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Service Manual
Copyright
2008 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.
Release, amendment, reproduction, distribution, rent, adaption and translation of this manual
in any manner whatsoever without the written permission of Mindray is strictly forbidden.
All information contained in this manual is believed to be correct. Mindray shall not be liable
for errors contained herein nor for incidental or consequential damages in connection with the
furnishing, performance, or use of this manual.
Mindray is responsible for safety, reliability and performance of this product only in the
condition that:
the electrical installation of the relevant room complies with the applicable national
and local requirements;
I
z This equipment must be operated by skilled/trained medical professionals.
z Be sure to operate the analyzer under the situation specified in this manual;
otherwise, the analyzer will not work normally and the analysis results will
be unreliable, which would damage the analyzer components and cause
personal injury.
II
Warranty
Exemptions
Mindray's obligation or liability under this warranty does not include any transportation or
other charges or liability for direct, indirect or consequential damages or delay resulting from
the improper use or application of the product or the use of parts or accessories not approved
by Mindray or repairs by people other than Mindray authorized personnel.
any Mindray product which has been subjected to misuse, negligence or accident;
any Mindray product from which Mindray's original serial number tag or product
identification markings have been altered or removed;
Return Policy
Return Procedure
In the event that it becomes necessary to return this product or part of this product to Mindray,
the following procedure should be followed:
1. Obtain return authorization: Contact the Mindray Service Department and obtain a
Customer Service Authorization (Mindray) number. The Mindray number must
appear on the outside of the shipping container. Returned shipments will not be
accepted if the Mindray number is not clearly visible. Please provide the model
number, serial number, and a brief description of the reason for return;
2. Freight policy: The customer is responsible for freight charges when this product is
shipped to Mindray for service (this includes customs charges);
3. Return address: Please send the part(s) or equipment to the address offered by
Customer Service department.
III
Company Contact
IV
Table of Contents
Copyright I
Warranty.............................................................................................................................III
Return Policy......................................................................................................................III
Table of Contents 1
1
Using This Manual
5 Servicing 5-1
5.1 General ................................................................................................................ 5-1
5.2 Disassembling the Panels ................................................................................... 5-2
5.3 Replacing the Power Supply Assembly ............................................................. 5-12
5.4 Replacing the Touchscreen Assembly............................................................... 5-16
5.5 Replacing the Fluidic Components.................................................................... 5-22
5.6 Replacing the RBC Assembly............................................................................ 5-56
5.7 Replacing the HGB Bath Assembly ................................................................... 5-66
5.8 Replacing the WBC Bath Assembly .................................................................. 5-70
5.9 Replacing the Reagent Preheating Assembly ................................................... 5-76
5.10 Replacing the Open Vial Sampling/SRV Assembly ........................................... 5-78
5.11 Replacing the Sample Transmitting Assembly .................................................. 5-96
5.12 Replacing the Autosampler and Mixer Assemblies ......................................... 5-115
5.13 Replacing the Pneumatic Unit ......................................................................... 5-130
5.14 Replacing the Ambient Temperature Sensor................................................... 5-138
5.15 Replacing the Blood Sensor ............................................................................ 5-140
5.16 Replacing the Filtering Cartridge ..................................................................... 5-142
7 Troubleshooting 7-1
7.1 Error code and information.................................................................................. 7-1
7.2 Causes of Common Errors .................................................................................. 7-6
7.3 Function Sequence Codes and Information ...................................................... 7-14
7.4 Error analysis and processing ........................................................................... 7-16
9 Appendices A-1
2
1 Using This Manual
1.1 Introduction
The chapter explains how to use the BC-5800 service manual. This manual provides the
reference information and procedures needed in servicing the BC-5800. Before servicing the
BC-5800, read and understand the manual carefully to ensure the proper servicing of the
equipment and personnel safety.
This manual is to be used together with the operation manual of BC-5800. It does not contain
information and procedures already covered in the operation manual of BC-5800.
1-1
Using This Manual
To use this manual effectively, you need to have the following capacities:
The ability to use basic mechanical tools and understand related terminology;
1-2
Using This Manual
This operation manual comprises 8 chapters and 5 appendices. Refer to the table below to find
the information you need.
1-3
Using This Manual
This manual uses certain typographical conventions to clarify meaning in the text:
Format Meaning
[XX] all capital letters enclosed in [ ] indicate a key name (either on
the pop-up keyboard or the external keyboard)
XX letters included in " " indicate text you can find on the screen
of BC-5800
XX italic letters indicate titles of the chapters that are referred to
XX all-capitalized, bold and italic letters indicate a major operation
defined in Section 1.4
All illustrations in this manual are provided as examples only. They may not necessarily reflect
your analyzer setup or data displayed.
1-4
Using This Manual
1-5
Using This Manual
1.6 Symbols
You may find the following symbols on the analyzer, reagents, controls or calibrators.
BIOLOGICAL RISK
HIGH VOLTAGE
1-6
Using This Manual
EARTH (GROUND)
ALTERNATING CURRENT
TYPE B DEVICE
BATCH CODE
USE BY (YYYY-MM-DD)
SERIAL NUMBER
MEASUREMENT AUTHORIZATION
SYMBOL
DATE OF MANUFACTURE
MANUFACTURER
TEMPERATURE LIMITATION
Be sure to observe the following precautions for the safety of patients, operators and yours
when you are servicing the analyzer.
1-7
Using This Manual
z When servicing the analyzer, be sure to turn off the power. Servicing the
analyzer when it is on may bring risk of electric shock or damage to
electronic components.
z Connect the analyzer to a socket having sole fuse and protective switch. Do
not use the same fuse and protective switch with other equipment (e.g. life
supporting equipment). Otherwise, the equipment failure, over current or
impulse current that occurs at the startup moment may lead to tripping.
z To prevent personal injury during maintenance, keep your clothes, hairs and
hands from the moving parts, such as sample probe, clipper and piercer.
z The reagents are irritating to eyes, skin and mucosa. Wear proper personal
protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory
procedures when handling them in the laboratory.
z If the reagents accidentally spill on your skin, wash them off with plenty of
water and if necessary, go see a doctor; if the reagents accidentally spill into
your eyes, wash them off with plenty of water and immediately go see a
doctor.
1-8
Using This Manual
z All the analyzer components and surfaces are potentially infectious. Take
proper protective measures for operation or maintenance.
z The sample probe tip is sharp and may contain biohazardous materials.
Exercise caution to avoid contact with the probe when working around it.
1-9
2 System Structure
Hardware
Hardware is designed for signal gathering, the controlling and driving of motion components
and the processing and display of data. It is mainly composed of main board, power drive
board, signal processing board, power board, blood sensor board and pressure sensor
board.
Interfaces
BC-5800 offers many interfaces to facilitate data processing and selection of various
accessories. It provides 4 USB interfaces, 1 network interface, 1 pneumatic unit control
interface and 1 reserved interface. USB interfaces can be used to connect mouse, keyboard,
printer, scanner and USB, and to perform software upgrade. The network interface adopts
built-in network card, can perform networking function.
System software can be divided into boot software, operation software and application
software. The boot software and operation software are stored in the FLASH chip of the main
board, and the application software is stored in the hard disk. The boot software has two main
functions, one is to configure address space, and the other is to initialize system memory.
The operation system completes the resource initialization of CPU board and provides
operation system environment to the application software. The application software offers an
interactive operation interface.
Fluidic system
Fluidic system is the tubing structure of the analyzer where reagent, sample and air flow
through. All hydraulic parts are connected correspondingly, and they are controlled by
software and drive hardware according to the specified order to realize control over the
allocation and direction of media.
2-1
System Structure
2-2
System Structure
2-3
System Structure
BC-5800 5-differential auto hematology analyzer consists of the main unit (analyzer),
pneumatic unit and accessories.
2-4
System Structure
2-5
System Structure
1
2
21 3
20
19
18 4
17 5
16
15
14
13 12 11 10 9 8 7 6
2-6
System Structure
2-7
System Structure
Figure 2-5 Front of the Analyzer (Front Cover Removed, Pierce & Mix Unit Configured)
2-8
System Structure
8 7 6 5
2-9
System Structure
9 10 11 12
7
6
2-10
System Structure
2-11
System Structure
Software system can be divided into boot software, operation software and application
software. Boot software is autoloaded by hardware; its code runs in FLASH to configure
address space and to initialize system memory. Operation system completes the resource
initialization of CPU board, loading application software and transferring the CPU control
authority to application software. The code of application software runs in SDRAM, it obtains
the resource data needed from external hard disk to provide functions like counting,
parameter setting, quality control, device maintenance, data management, assistance, help,
etc. The application software sends control command to corresponding fluidic units at the
specified time according to the fluidic unit control sequence in basic sequence.
2. Counting screen
Counting screen comprises four parts: status region, measurement result and data region of
the current sample, data display region of the next sample and function button region.
Operators can click each function button in the counting screen to perform the functions of
the analyzer.
See Figure 2-10 for the menu structure of the counting screen:
2-12
System Structure
3. Review screen
There are two modes of sample results review, table review mode and graph review mode.
When entering review screen from other screens, operators see the list review screen first.
See below for the menu structure of each screen:
Table review
2-13
System Structure
See Figure 2-11 for the menu structure of the table review menu:
Graph review
See Figure 2-12 for the menu structure of the graph review mode:
2-14
System Structure
L-J QC
2-15
System Structure
X-B QC
5. "Service" screen
"Service" screen includes "Maintenance", "Status", "Self-test", "Log", "Debug" and
"Initialization" screens. When switching to "Service" screen from other screens, operators
see the "Maintenance" screen first. See below for the menu structure of each screen:
2-16
System Structure
"Maintenance" screen
See Figure 2-15 for the menu structure of the "Maintenance" screen:
"Status" screen
See Figure 2-16 for the menu structure of the "Status" screen:
2-17
System Structure
Service Main
Count
Maintain
Status Version
Self-test Temp&Pres
Log Vol.&Cur.
Init. Position
"Self-test" screen
See Figure 2-17 for the menu structure of the "Self-test" screen:
2-18
System Structure
"Log" screen
See Figure 2-18 for the menu structure of the "Log" screen:
2-19
System Structure
"Debug" screen
See Figure 2-19 for the menu structure of the "Debug" screen:
2-20
System Structure
"Initialization" screen
See Figure 2-20 for the menu structure of the "Initialization" screen:
2-21
System Structure
Service Main
Count
Maintain
Status
Self-test
Log
7. "Setup" screen
"Setup" screen includes "User", "Setting" and "Advance" screens. When switching to
"Setup" screen from other screen, operators see "Setting" screen first. See below for the
menu structure of each function screen:
Setting
See Figure 2-21 for the menu structure of the "Setting" screen:
2-22
System Structure
2-23
System Structure
Advanced
See Figure 2-22 for the menu structure of the "Advanced" screen:
Advanced Main
Count
User
Setting
Advanced Service
User Management
See Figure 2-23 for the menu structure of the "User" screen:
8. Calibration screen
2-24
System Structure
2.5.2 Password
There are three levels of password: common user level, user administrator level and service
engineer level. The authorities of user administrator level cover all those of the common user
level, and the authorities of the service engineer level cover all those of the user administrator
level. See Table 2-1 for the permitted functions under each level of password.
2-25
System Structure
2-26
System Structure
2-27
System Structure
2-28
3 Fluidic System
3.1.1 Needles
Sample probe: connects to SRV (Sample Rotary Valve) for sample aspiration under
open vial mode.
Sample flow probe: connects to flow cell for the formation of sample flow in WBC
detection unit.
Probe wipe for piercing needle: locates in piercing mechanism, its function is to
wash the interior and exterior of the piercing needle.
3.1.3 Pumps
Metering pump: metering part, aspirates fluid by negative pressure, and then
switches to negative pressure to drain fluid, providing reagent of a predetermined
amount for sample analysis. For specific models and function, please refer to
Appendix D3.
3.1.4 Valves
Fluidic valve: controls fluid or air flow direction.
Pinch valve: starts/stops the fluid flow. For the quantity and function, please refer to
Appendix D4.
3-1
Fluidic System
Check valve: controls the unidirectional flow of fluid or air. For the quantity and
function, please refer to Appendix D5. See Figure 3-1 for the direction of check
valve.
SRV: metering part, provides a pre-determined amount of sample for analysis. See
Figure 3-2 - Figure 3-4.
3-2
Figure 3-2 Open vial feeding position SRV fluidic status
3-3
Fluidic System
3-4
Fluidic System
Serial Serial
Hole position Hole position
No. number
5. 17.
BASO 20ul (open vial)
6. 18. RBC sample (first dilution) 62.5ul
FCM cistern: sheath fluid (diluent) cistern, for WBC detection and cleaning.
WC1 waste cistern: waste cistern, gathering waste of WC2, WC3 and other
channels.
WC3 waste cistern: waste cistern, providing stable negative pressure during RBC
counting and gathering waste of RBC unit.
3-5
Fluidic System
3.1.6 Baths
WBC bath: includes DIFF bath and BASO bath; DIFF bath is for the reaction of WBC
4-differential sample,
BASO bath is for the reaction of sample testing basophils.
Flow cell: it is the part facilitating WBC testing; sheath fluid forms here.
RBC premix bath: RBC channel, it is for the first dilution of sample in RBC channel.
RBC bath: RBC/PLT detection unit where the test on RBC/PLT completes.
HGB bath: HGB detection unit where the HGB test completes.
3-6
Fluidic System
3-7
Fluidic System
3.2.3 Valves
Air valve: controls the directional flow of air.
Pressure regulator: regulates pressure coming out directly from the pneumatic unit to
meet requirement on all kinds of pressure.
3.2.4 Filters
Pressure filter: filtrates and dries the air with pressure coming directly out from the
pneumatic unit.
Small filter: connects to FCM cistern, filters small impurities in diluent coming out
from FCM cistern.
3-8
Fluidic System
The analysis modes include open vial whole blood, autoloading whole blood and
predilute mode, each mode is divided into CBC+5DIFF and CBC, so there are
altogether 6 modes.
CBC+5DIFF mode
Autoloading Predilute
Open vial
Reagents Name whole
whole blood
blood
M-58 diluent M-58D diluent 58mL 60ml 58ml
M-58LEO(I) lyse 1.56mL 1.56mL 1.56mL
M-580LEO(II) lyse 0.32mL 0.32mL 0.32mL
M-58 lyse
M-58LBA lyse 1.56mL 1.56mL 1.56mL
M-58LH lyse 1.0mL 1.0mL 1.0mL
CBC mode
Autoloading Predilute
Open vial
Reagents Name whole
whole blood
blood
M-58 diluent M-58D diluent 48mL 50ml 48mL
M-58LEO(I) lyse - - -
M-58LEO(II) lyse - - -
M-58 lyse
M-58LBA lyse 1.56mL 1.56mL 1.56mL
M-58LH lyse 1.0mL 1.0mL 1.0mL
3-9
Fluidic System
Shutdown function
Reagent volume
Reagents Name required when shutting
down normally
M-58 diluent M-58D diluent 11ml
M-58LEO(I) lyse -
M-58LEO(II) lyse -
M-58 lyse
M-58LBA lyse -
M-58LH lyse -
Cleanser for 49ml
M-58 cleanser
hospital use
3-10
Fluidic System
BASO Channel
Reagents used:
LBA lyse: lyses RBC, PLT and turns WBC (excluding basophils) into naked nucleus cells;
Diluent: cleaning, and providing sheath fluid
Function description: the sample is diluted in the bath to the ratio of 1:52, and then
the dilution moves to the flow cell. Meanwhile sheath fluid is started and stabilized.
After that, the dilution moves to the measuring segment of the flow cell. Sample
syringe pushes the dilution, which is surrounded by the sheath fluid, through the flow
cell for optical measurement at a stable speed within certain amount of time. Then
clean the measurement tube to restore its original status.
3-11
Fluidic System
DIFF Channel
Reagents used:
LEO(I) and LEO(II): lyses RBC and performs dissimilation treatment to various types of WBC;
Diluent: cleaning, and providing sheath fluid
Function description: the sample is diluted in the bath to the ratio of 1:68, and then
the dilution moves to the flow cell. Meanwhile sheath fluid is started and stabilized.
After that, the dilution moves to the measuring segment of the flow cell. Sample
syringe drives the dilution, which is surrounded by the sheath fluid, through the flow
cell for optical measurement at a stable speed within certain amount of time. Then
clean the measurement tube to restore its original status.
Mix: mix the sample with reagent by jet flow produced when dispensing the sample
and by using the mixing bar in DIFF and BASO bath.
Clean: clean the bath with fluid in DIL cistern through energizing fluidic valve SV17
and pinch valve PV11 and PV12.
Waste drainage: drain the waste with WC1 vacuum by energizing SV30 and SV31.
RBC/PLT Channel
Reagents used:
Diluent: diluting and cleaning, providing conductive environment and isovolumetric treatment
to cells
3-12
Fluidic System
The function of RBC channel is to count RBC and PLT in blood sample, the method adopted is
impedance method. During the detection, blood sample needs to be diluted to the ratio of
1:18000, which is hard to achieve with a single dilution, so the second dilution needs to be
conducted. First dilute the sample to the ratio of 1:433 and dispense it in to the premix bath;
and then aspirate the 1:433 sample back into the SRV and redilute it to the ratio of 1:41.6,
finally dispense it into RBC bath to form the 1:18000 sample.
Clean: add diluent into the RBC counting bath through DP8, SV22 and SV05 to clean
the bath; add diluent in to the premix bath through DP6 and SV23 to clean the bath;
Waste draining: RBC premix bath drains waste by WC1 vacuum and SV29; RBC
counting bath drains waste by WC1 vacuum and SV36;
3-13
Fluidic System
bath through DP7 and SV19, meanwhile dispense certain amount of lyse into the
bath through DP5 and SV28;
Clean: dispense diluent into the HGB bath through DP7 and SV19 to clean the bath;
3-14
Fluidic System
Figure 3-8 The fluidic connection chart of cistern and waste channels
Cleanser cleaning procedure: energize fluid valve SV13 and the cleanser control
valves of all baths, dispense certain amount of cleanser into the baths by pressure to
clean them.
3-15
Fluidic System
SRV is at the working position, that is SRV bar stays at the upper position.
During 0-3.6s, aspirate the sample to SRV from manual sample flow probe through
whole blood syringe SV09 and SV10;
Start probe wipe to clean the exterior of the open vial sample probe;
Empty RBC premix bath, RBC bath, HGB bath, DIFF and BASO bath;
Add 2ml diluent to HGB bath through DP7 and SV19, wait for 3.5s until the dilution
becomes stable, measure background voltage and then empty HGB bath;
Dispense diluent into RBC bath through DP8, SV22 and SV5 to clean the bath and
then empty it;
Dispense LEO I lyse and LBA lyse into DIFF bath and BASO bath respectively to
clean the baths, then empty them;
WC2-1
T20 M-50D T2
T44
LEO(I) T4
T45
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
SV14
T200
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
GV66 G1
T80
T117
E1
T165
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
C130
T83
SV5 C60
0.07MPa
J78-T147
C61
J73-T167-J74
T119 T118
T148-P4-P3
T66
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
0.07MPa GV67
0.07MPa
J77-T146
VAC
T136 J79-T137
T105
2.6mL
T143
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
J69-T98-J70
PV4/GV56
T205-J88-T206-J87-T207 C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Figure 3-9 Aspiration fluidic status under open vial whole blood mode
3-16
Fluidic System
SRV switches to the lower position, that is the SRV bar is at the lower position; That
means the SRV has obtained samples of 4 portions needed for the measurement;
Dispense RBC sample into premix bath through DP8 and SV22 to dilute it for the first
time, and then aspirate the diluted sample back into aspiration channel through DP6;
Dispense HGB sample into HGB bath through DP7 and SV19, meanwhile add lyse
into HGB bath through DP5 and SV28 to mix with the sample;
Dispense DIFF sample into DIFF bath through DP2 and SV26;
Dispense BASO sample into BASO bath through DP3 and SV27;
WC2-1
T20 M-50D T2
T44
T14
LEO(I) T4
T45
T22
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
C3 C6 C7 C8 C11 C14 LEO(II) 0.32mL
T182 J3-T178-J4 J5-T177-J6 T175 J15-T171-J16 J17-T168-J18 T152 T154 T155
C2 C4 C10 C13 SV13 C47
J9-T176-J10
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
T52
SV14
T200
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
C16 T90-P7 T91-J98
G2
T54
GV66 G1
T80
T117
E1
T165
-0.04MPa
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
C130
T83
SV5 C60
0.07MPa
J78-T147
C61
J73-T167-J74
T119 T118
T148-P4-P3
T67
T69
T68
T103-J67-T102-J68-T101
T66
C23 C22 T188
GF3
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
0.07MPa GV67
0.07MPa
PV5-
PV4-
PV3-
J77-T146
VAC
T348
0.07MPa
T193
T136 J79-T137
T105
2.6mL
T143
T192
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
J69-T98-J70
PV4/GV56
T205-J88-T206-J87-T207 C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Figure 3-10 Drainage fluidic status under open vial whole blood mode
Deliver the diluted sample (first dilution) that is measured by SRV to RBC bath
through DP8, SV22 and SV5;
Deliver the sample in DIFF bath to the inlet of the flow cell through DP4, SV7, PV10
and PV11, start sheath fluid through SV3;
3-17
Fluidic System
T43 J27-T351-J104
C12 T13 M-50 T1
C5 LF1 T19 C45
T18
WC2-2
WC2-1
T20 M-50D T2
T44
GV78 GV80 LBA T3
SV15 GV79
T14
LEO(I) T4
T45
BASO DIFF VAC CV1 VAC
CV2 VAC LEO(II) T5
T22
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T160 J51-T30-J52 0.07MPa
T47
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
C3 C6 C7 C8 C11 C14 LEO(II) 0.32mL
T182 J3-T178-J4 J5-T177-J6 T175 J15-T171-J16 J17-T168-J18 T152 T154 T155
C2 C4 C10 C13 SV13 C47
J9-T176-J10
T156
SV11 SV26 VAC
T51
VAC 1 2 DP2 GV71
T183
T161
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
T52
SV14
T200
T157
0.07MPa T73-J57 T72 C49 OPEN T61
SV24 SV34
T149
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
C16 T90-P7 T91-J98
G2
T54
GV66 G1
T80
T117
E1
T165
J81-T95 T87-J60 C40
-0.04MPa
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
SV29 A2 A1 T85 T195
T112-J83-T113-J82-T114 T94-J64 T93
C C52 C58 C64
T82
C130
T83
SV5 C60
0.07MPa
J78-T147
C61
J73-T167-J74
T204 T203 T120 T119 T118 T148-P4-P3 SV16 WC1
T67
T69
T68
T103-J67-T102-J68-T101
T66
C23 C22 T188
GF3
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
T106
0.07MPa GV67
0.07MPa
PV5-
PV4-
PV3-
J77-T146
VAC
T348
0.07MPa
T193
T136 J79-T137
T105
2.6mL
T143
T192
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
T205-J88-T206-J87-T207
PV4/GV56 J69-T98-J70
C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Figure 3-11 The first dilution RBC sample preparation fluidic status under open vial
whole blood mode
Start sample syringe, drive the DIFF sample slowly into the optical detection area of
the flow cell for measurement;
Empty waste and clean DIFF bath, prime 1.5ml diluent into DIFF bath through SV17
and PV11 with the effect of DIL cistern pressure. DIFF bath is ready for
measurement.
Empty premix bath through SV11, dispense cleanser into premix bath through DP6 to
clean the bath;
3-18
Fluidic System
T43 J27-T351-J104
C12 T13 M-50 T1
C5 LF1 T19 C45
T18
WC2-2
WC2-1
T20 M-50D T2
T44
GV78 GV80 LBA T3
SV15 GV79
T14
LEO(I) T4
T45
BASO DIFF VAC CV1 VAC
CV2 VAC LEO(II) T5
T22
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T160 J51-T30-J52 0.07MPa
T47
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
C3 C6 C7 C8 C11 C14 LEO(II) 0.32mL
T182 J3-T178-J4 J5-T177-J6 T175 J15-T171-J16 J17-T168-J18 T152 T154 T155
C2 C4 C10 C13 SV13 C47
J9-T176-J10
T156
SV11 SV26 VAC
T51
VAC 1 2 DP2 GV71
T183
T161
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
T52
SV14
T200
T157
0.07MPa T73-J57 T72 C49 OPEN T61
SV24 SV34
T149
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
C16 T90-P7 T91-J98
G2
T54
GV66 G1
T80
T117
E1
T165
J81-T95 T87-J60 C40
-0.04MPa
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
SV29 A2 A1 T85 T195
T112-J83-T113-J82-T114 T94-J64 T93
C C52 C58 C64
T82
C130
T83
SV5 C60
0.07MPa
J78-T147
C61
J73-T167-J74
T204 T203 T120 T119 T118 T148-P4-P3 SV16 WC1
T67
T69
T68
T103-J67-T102-J68-T101
T66
C23 C22 T188
GF3
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
T106
0.07MPa GV67
0.07MPa
PV5-
PV4-
PV3-
J77-T146
VAC
T348
0.07MPa
T193
T136 J79-T137
T105
2.6mL
T143
T192
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
T205-J88-T206-J87-T207
PV4/GV56 J69-T98-J70
C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Figure 3-12 DIFF measurement preparation fluidic status under open vial whole blood
mode
Open vial sample probe and probe wipe restore to the ready-for-detection status,
prepare next sample;
Wait for 19s so that the HGB sample turns stable, start measurement, and then
empty HGB bath after the measurement completes;
Clean WBC sample preparation tubes through SV1, PV10, SV7 and SV8;
DIL obtains diluent from diluent container with the effect of vacuum through PV7 and
GV79.
3-19
Fluidic System
T43 J27-T351-J104
C12 T13 M-50 T1
C5 LF1 T19 C45
T18
WC2-2
WC2-1
T20 M-50D T2
T44
GV78 GV80 LBA T3
SV15 GV79
T14
LEO(I) T4
T45
BASO DIFF VAC CV1 VAC
CV2 VAC LEO(II) T5
T22
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T160 J51-T30-J52 0.07MPa
T47
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
C3 C6 C7 C8 C11 C14 LEO(II) 0.32mL
T182 J3-T178-J4 J5-T177-J6 T175 J15-T171-J16 J17-T168-J18 T152 T154 T155
C2 C4 C10 C13 SV13 C47
J9-T176-J10
T156
SV11 SV26 VAC
T51
VAC 1 2 DP2 GV71
T183
T161
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
T52
SV14
T200
T157
0.07MPa T73-J57 T72 C49 OPEN T61
SV24 SV34
T149
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
C16 T90-P7 T91-J98
G2
T54
GV66 G1
T80
T117
E1
T165
J81-T95 T87-J60 C40
-0.04MPa
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
SV29 A2 A1 T85 T195
T112-J83-T113-J82-T114 T94-J64 T93
C C52 C58 C64
T82
C130
T83
SV5 C60
0.07MPa
J78-T147
C61
J73-T167-J74
T204 T203 T120 T119 T118 T148-P4-P3 SV16 WC1
T67
T69
T68
T103-J67-T102-J68-T101
T66
C23 C22 T188
GF3
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
T106
0.07MPa GV67
0.07MPa
PV5-
PV4-
PV3-
J77-T146
VAC
T348
0.07MPa
T193
T136 J79-T137
T105
2.6mL
T143
T192
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
T205-J88-T206-J87-T207
PV4/GV56 J69-T98-J70
C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Figure 3-13 RBC and DIFF measurement preparation fluidic status under open vial
whole blood mode
Add 4ml diluent into HGB bath through DP7 and SV19, HGB bath is ready for
measurement.
Deliver the sample in BASO bath to the inlet of the flow cell through DP4, SV7, PV10
and PV12, start sheath fluid through SV3;
Start sample syringe, drive the BASO sample slowly into the optical detection area of
the flow cell for measurement; Empty waste and clean BASO bath;
After clean the RBC premix bath, add 2ml diluent through DP6 and SV23. RBC
premix bath is ready for measurement;
Empty RBC bath, add 2.6ml diluent through DP8 and SV22 to clean the bath.
3-20
Fluidic System
T43 J27-T351-J104
C12 T13 M-50 T1
C5 LF1 T19 C45
T18
WC2-2
WC2-1
T20 M-50D T2
T44
GV78 GV80 LBA T3
SV15 GV79
T14
LEO(I) T4
T45
BASO DIFF VAC CV1 VAC
CV2 VAC LEO(II) T5
T22
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T160 J51-T30-J52 0.07MPa
T47
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
C3 C6 C7 C8 C11 C14 LEO(II) 0.32mL
T182 J3-T178-J4 J5-T177-J6 T175 J15-T171-J16 J17-T168-J18 T152 T154 T155
C2 C4 C10 C13 SV13 C47
J9-T176-J10
T156
SV11 SV26 VAC
T51
VAC 1 2 DP2 GV71
T183
T161
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
T52
SV14
T200
T157
0.07MPa T73-J57 T72 C49 OPEN T61
SV24 SV34
T149
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
C16 T90-P7 T91-J98
G2
T54
GV66 G1
T80
T117
E1
T165
J81-T95 T87-J60 C40
-0.04MPa
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
SV29 A2 A1 T85 T195
T112-J83-T113-J82-T114 T94-J64 T93
C C52 C58 C64
T82
C130
T83
SV5 C60
0.07MPa
J78-T147
C61
J73-T167-J74
T204 T203 T120 T119 T118 T148-P4-P3 SV16 WC1
T67
T69
T68
T103-J67-T102-J68-T101
T66
C23 C22 T188
GF3
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
T106
0.07MPa GV67
0.07MPa
PV5-
PV4-
PV3-
J77-T146
VAC
T348
0.07MPa
T193
T136 J79-T137
T105
2.6mL
T143
T192
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
T205-J88-T206-J87-T207
PV4/GV56 J69-T98-J70
C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Figure 3-14 BASO sample preparation fluidic status under open vial whole blood mode
Dispense 1.5ml diluent into BASO bath through SV17 and PV12. BASO bath is ready
for measurement.
3-21
Fluidic System
T43 J27-T351-J104
C12 T13 M-50 T1
C5 LF1 T19 C45
T18
WC2-2
WC2-1
T20 M-50D T2
T44
GV78 GV80 LBA T3
SV15 GV79
T14
LEO(I) T4
T45
BASO DIFF VAC CV1 VAC
CV2 VAC LEO(II) T5
T22
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T160 J51-T30-J52 0.07MPa
T47
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
C3 C6 C7 C8 C11 C14 LEO(II) 0.32mL
T182 J3-T178-J4 J5-T177-J6 T175 J15-T171-J16 J17-T168-J18 T152 T154 T155
C2 C4 C10 C13 SV13 C47
J9-T176-J10
T156
SV11 SV26 VAC
T51
VAC 1 2 DP2 GV71
T183
T161
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
T52
SV14
T200
T157
0.07MPa T73-J57 T72 C49 OPEN T61
SV24 SV34
T149
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
C16 T90-P7 T91-J98
G2
T54
GV66 G1
T80
T117
E1
T165
J81-T95 T87-J60 C40
-0.04MPa
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
SV29 A2 A1 T85 T195
T112-J83-T113-J82-T114 T94-J64 T93
C C52 C58 C64
T82
C130
T83
SV5 C60
0.07MPa
J78-T147
J73-T167-J74 C61
T119 T118
T148-P4-P3
T67
T69
T68
T103-J67-T102-J68-T101
T66
C23 C22 T188
GF3
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
T106
0.07MPa GV67
0.07MPa
PV5-
PV4-
PV3-
J77-T146
VAC
T348
0.07MPa
T193
T136 J79-T137
T105
2.6mL
T143
T192
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
J69-T98-J70
PV4/GV56
T205-J88-T206-J87-T207 C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Figure 3-15 BASO measurement fluidic status under open vial whole blood mode
Clean WBC sample preparation tubes through SV1, PV10, SV7 and SV8 with the
effect of FCM pressure;
DIL obtains diluent from diluent container through PV7 and GV79 with the effect of
vacuum;
FCM obtains diluent from diluent container through PV8 and GV80 with the effect of
vacuum.
WC1 drains waste into the waste container outside the instrument.
3-22
Fluidic System
T43 J27-T351-J104
C12 T13 M-50 T1
C5 LF1 T19 C45
T18
WC2-2
WC2-1
T20 M-50D T2
T44
GV78 GV80 LBA T3
SV15 GV79
T14
LEO(I) T4
T45
BASO DIFF VAC CV1 VAC
CV2 VAC LEO(II) T5
T22
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T160 J51-T30-J52 0.07MPa
T47
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
C3 C6 C7 C8 C11 C14 LEO(II) 0.32mL
T182 J3-T178-J4 J5-T177-J6 T175 J15-T171-J16 J17-T168-J18 T152 T154 T155
C2 C4 C10 C13 SV13 C47
J9-T176-J10
T156
SV11 SV26 VAC
T51
VAC 1 2 DP2 GV71
T183
T161
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
T52
SV14
T200
T157
0.07MPa T73-J57 T72 C49 OPEN T61
SV24 SV34
T149
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
C16 T90-P7 T91-J98
G2
T54
GV66 G1
T80
T117
E1
T165
J81-T95 T87-J60 C40
-0.04MPa
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
SV29 A2 A1 T85 T195
T112-J83-T113-J82-T114 T94-J64 T93
C C52 C58 C64
T82
C130
T83
SV5 C60
0.07MPa
J78-T147
C61
J73-T167-J74
T204 T203 T120 T119 T118 T148-P4-P3 SV16 WC1
T67
T69
T68
T103-J67-T102-J68-T101
T66
C23 C22 T188
GF3
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
T106
0.07MPa GV67
0.07MPa
PV5-
PV4-
PV3-
J77-T146
VAC
T348
0.07MPa
T193
T136 J79-T137
T105
2.6mL
T143
T192
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
T205-J88-T206-J87-T207
PV4/GV56 J69-T98-J70
C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Figure 3-16 BASO measurement fluidic status under open vial whole blood mode
The open vial prediluted measurement cycle is completely the same as open vial whole blood
measurement cycle; see 3.4.1 for the detailed process;
The difference between the two modes: the sample under predilute mode is diluted according
to certain ratio outside the instrument; while the sample under whole blood mode is
unprediluted whole blood.
3-23
Fluidic System
T43 J27-T351-J104
C12 T13 M-50 T1
C5 LF1 T19 C45
T18
WC2-2
WC2-1
T20 M-50D T2
T44
GV78 GV80 LBA T3
SV15 GV79
T14
LEO(I) T4
T45
BASO DIFF VAC CV1 VAC
CV2 VAC LEO(II) T5
T22
0.16MPa
T23
0.07MPa
T181
C36 0.07MPa LH T6
T38-J105
J43-T29-J44
T15
T46
SV3 SV2
T201
SV6
J45-T11-J46
J47-T10-J48
J41-T28-J42
LEO(II)
T185
PV8/GV53 PV7/GV54
J49-T9-J50
T202
LBA
LEO(I)
M-50
T169-T170
T8
LH
T12
T172-T173
T7
J1-T180-J2 C1
J7-T49-J8
J96-T31-J95
M-50
LH
FCM DIL
J39-T27-J40
T179
SV8 SV1
J37-T26-J38
T216-P2
T215-P1
J11-T48-J12
PV12/GV50 PV11/GV51
T197
T198
T160 J51-T30-J52 0.07MPa
T47
T36
T184 SV25 VAC
C38
J13-T174-J14
DP1 GV69
J21-T153-J22
SV7 T50
T38-J28-T37-J29
C3 C6 C7 C8 C11 C14 LEO(II) 0.32mL
T182 J3-T178-J4 J5-T177-J6 T175 J15-T171-J16 J17-T168-J18 T152 T154 T155
C2 C4 C10 C13 SV13 C47
J9-T176-J10
T156
SV11 SV26 VAC
T51
VAC 1 2 DP2 GV71
T183
T161
SV18
VAC
S_SYRINGE
SV27
DP4 J25-T42-J26 J32-T41 DP3
100L
T52
SV14
T200
T157
0.07MPa T73-J57 T72 C49 OPEN T61
SV24 SV34
T149
T62
T76
0.07MPa
T77 C50
T78
T199
T53
WC2-2
WC2-1
T164
T79-J80
C51
DP6 L M
T74-J58 T75 PV3-
1mL I1 T88-J59
I2 PV3/GV57
T63
C16 T90-P7 T91-J98
G2
T54
GV66 G1
T80
T117
E1
T165
J81-T95 T87-J60 C40
-0.04MPa
E2
0.07MPa
T89-P8 T92-J97
H WC2
C20 C57
T55
T116
C17 T60
RBC C56
T81
T56
GV77
K
T86
SV37 PV1/GV59
CV5 D2
D1
F2 T59 T64
T189
T166
J84-T115-J85
F1 C41 SV38
T191
T65
B2
T57
B1
T58
SV29 A2 A1 T85 T195
T112-J83-T113-J82-T114 T94-J64 T93
C C52 C58 C64
T82
C130
T83
SV5 C60
0.07MPa
J78-T147
C61
J73-T167-J74
T204 T203 T120 T119 T118 T148-P4-P3 SV16 WC1
T67
T69
T68
T103-J67-T102-J68-T101
T66
C23 C22 T188
GF3
C21 T187
C62 C63
SV22
J86-T138-P6 SV10 T190
T84
GV74
SV32
LH
T106
0.07MPa GV67
0.07MPa
PV5-
PV4-
PV3-
J77-T146
VAC
T348
0.07MPa
T193
T136 J79-T137
T105
2.6mL
T143
T192
C28 CV3
J91-T140-J92
T133
0.07MPa
VAC
HGB
T194
PV6/GV49
GV70
T210
T99
GV65 J65-T104-J66
T100
T132
T108
T109
PV5- T218
SV9 T107 T110 T111 C65
T142
C29 C55
J72-T126-P9
C54
0.07MPa
J71-T125
GV68
T205-J88-T206-J87-T207
PV4/GV56 J69-T98-J70
C33
P_SYRINGE
GV60
CV6
GV61
T124
2.5mL
T130
SV35 DP7
C24 J89-T208-J90 CV4
2mL
PV4-
SV20
T122 C43
T131 T121 T96
C34
Measurement process
The measurement process after sample is dispensed into SRV through whole blood syringe is
completely the same as the open vial whole blood measurement process;
SRV position
Measurement duration
Autoloading measurement mode realizes overall automatic functions like auto-loading and
auto-unloading of tubes and the auto-cycling of measurement process. The detection speed is
about 90 samples per hour.
3-24
Fluidic System
3-25
Fluidic System
3-26
4 Hardware System
According to function modules classification, the entire hardware system can be divided into
main board, signal processing board, driver board, touch screen control board, interface board,
indicator board, power board, pneumatic control board, pre-amplification board, laser control
board, volumetric board, etc. See Figure 4-1 for the block diagram of the entire hardware
system:
4-1
Hardware System
4-2
Hardware System
4.1.1 Overview
In BC-5800 5-differential hematology analyzer, main board is the hardware platform of the
operation system and application software, it controls the operation of the whole system. It
offers some external interfaces, such as USB interface, TTL serial port, RS232 serial port, IDE
interface, network interface, SPI interface, difference display interface, etc.
Section 4.1 of this manual is the servicing and troubleshooting guideline of the main board.
Realizes centralized management and overall coordination over all function boards.
Two RS232 serial ports and two TTL level serial ports;One LVDS level display
interface/one TFT LCD backlight interface;
One standard IDE data interface/one IDE hard disk power interface;
See below Figure 4-3 for the block diagram of the main board:
4-3
Hardware System
Description
Provides platform for the operation system and application software;
Realizes centralized management and overall coordination over all function boards.
Sends commands to signal processing board through SPI interface and receives responses
and sampling information from the signal processing board simultaneously;
Sends control commands to driver board through serial port and receives responses from the
driver board simultaneously;
The main board can upgrades the hardware of signal processing board and driver board
respectively through serial port and GPIO interface;
Two TTL level serial ports and two RS232 serial ports;
The main board communicates with the driver board through TTL level serial port, sends
control commands to the driver board and receives its responses. Its communication
parameters are:
Baud rate: 38400bps
Data bit: 8 bits
Stop bit: 1bit
Start bit: 1bit
Verification mode: even parity check
4-4
Hardware System
Communicates with and sends control commands to the signal processing board, and receive
its responses and sampling information simultaneously.
One standard IDE data interface/one IDE hard disk power interface
Connects the hard disk of the IDE interface. It supports 40G capacity and PIO mode. DMA
mode is not supported.
Connected to the backboard of the analyzer enclosure from the interface board, the USB
interfaces connect the USB mouse, keyboard, barcode reader and memory (USB flash drive /
portable disk).
Connected to the backboard of the analyzer enclosure from the interface board, the network
interface downloads program and uploads data.
It is used when debugging main board for hardware examination, program downloading and
FLASH programming. It is not open to users.
Interface Definition
The main board has 18 external interfaces with their number from J1-J18. Among the
interfaces, J7 is not to be used; J15 and J18 are reserved interfaces. The positions of all
interfaces on the board are portrayed as below Figure 4-4.
4-5
Hardware System
4-6
Hardware System
interface
USB interface to interface /
J14 24
board
Spare communication serial RS232 level
J15 5
port
J16 LCD backlight interface 5 /
J17 IDE power interface 5 /
J18 Spare I/O interface 8 /
J1 is a standard IDE interface; see Table 4-2 for the distribution and definition of its pins.
PIN PIN
Definition Function Definition Function
No. No.
1 nRESET Reset 21 NA Reserved
2 GND Signal ground 22 GND Signal ground
3 DD7 Data bit 7 23 nDIOW Write signal
4 DD8 Data bit 8 24 GND Signal ground
5 DD6 Data bit 6 25 nDIOR Read signal
6 DD9 Data bit 9 26 GND Signal ground
7 DD5 Data bit 5 27 NA Reserved
8 DD10 Data bit 10 28 GND Signal ground
9 DD4 Data bit 4 29 NA Reserve
10 DD11 Data bit 11 30 GND Signal ground
11 DD3 Data bit 3 31 INTRQ Interrupt request
12 DD12 Data bit 12 32 NA Reserved
13 DD2 Data bit 2 33 DA1 Register address 1
14 DD13 Data bit 13 34 NA Reserved
15 DD1 Data bit 1 35 DA0 Register address 0
16 DD14 Data bit 14 36 DA2 Register address 2
17 DD0 Data bit 0 37 nCS0 Chip selection 0
18 DD15 Data bit 15 38 nCS1 Chip selection 1
19 GND Signal ground 39 nDASP Sub-device effective
20 NA Reserved 40 GND Signal ground
J2 is the LVDS interface connecting to LCD, it has 20 pins. See Table 4-3 for its definition.
4-7
Hardware System
J3 interface is the power input port of the entire main board. It provides +5V and +12V power
supply, see Table 4-4 for its pin distribution.
J4 interface is the external communication interface connecting the signal processing board.
See Table 4-5 for its definition.
PIN
PIN No. Definition Function No. Definition Function
1 SPIOUT Data wire 11 DSCK Clock
Reference Reference
2 GND ground 12 GND ground
Configuration
3 SPISCK Clock 13 CONF_DONE completes
4 SPIIN Data wire 14 NA Reserved
SPI chip
5 SPICS0 selection 0 15 nCONFIG Configuration
Reference
6 GND ground 16 nCE Enable
4-8
Hardware System
J5 interface is the COM port 2 external interface connecting the driver board. See Table 4-6 for
definition of its pins.
J6 interface is reserved temporarily. See Table 4-7 for the definition of its pins.
4-9
Hardware System
J8 interface the external network interface that is connected to the interface board. See Table
4-8 for its definition.
J9 interface is the COM port 0 external interface connecting the barcode reader. See Table 4-9
for definition of its pins.
J10 interface is the COM port 1 external interface connecting the touchscreen controller. See
Table 4-10 for definition of its pins.
4-10
Hardware System
J12 interface is the JTAG interface of the FPGA; it is not open to customers.
J11 interface is the configured interface of the EPCS1; it is not open to customers.
J14 interface is the USB interface connecting the interface board. See Table4-11 for the
distribution of its pins.
PIN PIN
Definition Function Definition Function
No. No.
NA Reserved GND Reference
13
1 ground
NA Reserved GND Reference
14
2 ground
NA Reserved GND Reference
15
3 ground
NA Reserved DP2 Data positive
16
4 (port 2)
DM4 Data negative GND Reference
17
5 (port 4) ground
4-11
Hardware System
J16 interface is LCD backlight interface. See Table 4-12 for the distribution of its pins.
J17 is the power interface of IDE device. See Table 4-13 for the distribution of its pin.
4-12
Hardware System
Assembly drawing
See Figure 4-5 for the assembly drawing of the main board.
4-13
Hardware System
Indicator Function
D2 Reserved temporarily.
D3 +5V power indicator, lights up when power is on
D4 IDE device effectiveness indicator, lights up when the device
is in effect.
D7 Network full duplex communication indicator, lights up when
it is in effect.
D8 Network Collision indicator, lights up when it is in effect.
D9 Activating 100M network communication indicator, lights up
when it is in effect.
D10 Network connection indicator, lights up when it is in effect.
D11 Activating 10M network communication indicator, lights up
when it is in effect.
D12 Power indicator of the second USB port, lights up when
power is on.
D13 Power indicator of the fourth USB port, lights up when power
is on.
D14 Power indicator of the third USB port, lights up when power
is on.
D15 Power indicator of the first USB port, lights up when power is
on.
D16 Reserved for software use.
D17 Reserved for now.
D18 Reserved for software use.
D19 Reserved for now.
D20 FPGA configuration completion indicator, lights up when
configuration completes.
D21 Reserved for software use.
4-14
Hardware System
4-15
Hardware System
Key Definition
Table 4-16 Key Definition
Mark Function
S1 System manual reset
z Please make sure power is off and the power cord is disconnected when
mounting and disassembling boards.
4-16
Hardware System
Installation
Installation is the opposite operation of disassembling.
Verification
1 After powering on the device, D3 and D20 turn on;
2 After system initialization, indicator D12, D13, D14 and D15 turn on;
4-17
Hardware System
4.1.5 Troubleshooting
Table 4-17 below lists all common errors of the main board and their resolving methods from
hardware perspective. Software errors are excluded. But a lot of errors need to be checked
with software. Errors caused by CPU damage are not listed in Table 4-17.
Serial
Phenomenon Error analysis and processing
No.
1 Possible causes of the error: input power failure,
including power cords not well-connected,
3.3V/2.5V/1.5V/1.25V power supply voltage
abnormity, etc.; FLASH chip failure, operation system
cannot be imported into DDR SDRAM where it
operates; DDR SDRAM chip failure, the operation
system cannot run normally; system main clock
(CLKIN) abnormity.
2 Recommended action: press reset button to restart
the system, see whether the same error still exists;
1 System cannot start up check to see whether the power indicator D3 is on, or
measure input voltage with a multimeter to see
whether the input voltage is within normal range;
check whether the fuse F1 and F2 are burnt out;
Check whether the voltages of 3.3V/2.5V/1.5V/1.25V
power supply are within their normal operating range
with a multimeter; check whether the main clock
works normally with an oscilloscope; examine FLASH
or DDR with test software (Note: When testing
FLASH, the programs in it will be wiped off. Use this
method with extreme care.
1 Possible causes of the error: 12V power supply
does not supply power normally; the inverter is not
well connected; the enabling signal of pin 3 in J16
interface is low level (backlight on when the signal is
2 Blank screen
high level); FPGA does not return to original position
(system cannot start up); inverter failure, etc.
2 Recommended action: check with a multimeter and
oscilloscope.
1 Possible causes of the error: data wire is not well
Screen blur and color
3 connected; crystal oscillator X1/X6 does not work
cast
normally; FPAG configuration failure (indicator D20 is
4-18
Hardware System
4-19
Hardware System
U21 fails;
2 Recommended action: check whether the wires are
well connected and the interface board works
normally; check whether clock X2 is functioning with
an oscilloscope; examine network chip
U21(RTL8201);
Note: Generally errors occur in the network unit need
to be tested with software.
4-20
Hardware System
Function
Amplifying and collecting (LAS,MAS) WBC signals
Monitoring voltage (operating voltage, WBC/HGB blank voltage and RBC aperture
voltage)
See below Figure 4-7 for the block diagram of the signal processing board:
4-21
Hardware System
Description
Amplifying and collecting (LAS,MAS) WBC signals
The signal processing board first eliminates DC components from the WBC signals coming
from the pre-amplification board, and then amplifies, filters, buffers the signals, which are then
converted to digital signals, and sent to the FPGA for pulse identification.
The signal processing board amplifies (low noise, 2nd level and gain), buffers and A/D
converts the RBC signals, and then input the digital signals to FPGA for pulse identification.
The signal processing board converts the current signals of the HGB reaction bath to voltage
signals, then amplifies and converts the voltage signals to digital signals, which are then input
to the FPGA for inquiry of the main board.
While converting analog signals to digital signals, the FPGA identifies pulse signals and stores
the identified cell signal peaks in the SRAM.
Through the I/O interface of FPGA, the signal processing board controls several switches
(including consistent current source switch, zapping voltage switch, consistent current
source/zapping switch and HGB LED switch) and the volumetric board, and configures the
4-22
Hardware System
Through the SPI interface, the main board can control the signal processing board and
sampling, set/search for parameters, read data, etc.
Monitoring voltage
The signal processing board monitors the operating voltage, WBC/HGB blank voltage and
RBC aperture voltage through the shared A/D with the HGB board.
When the analyzer runs for a specific period of time or the RBC bath is blocked by foreign
matters, the signal processing board can zap the aperture, removing the dirty and foreign
matters.
Interface Definition
There are 8 interfaces on the signal processing board, which are LAS, MAS, RBC/PLT, HGB,
POWER, JTAG, VMB and MCB, as shown in Figure 4-8.
4-23
Hardware System
4-24
Hardware System
J1 is a LAS signal interface; see Table 4-19 for the distribution and definition of its pins.
J2 is a MAS signal interface; see Table 4-20 for the distribution and definition of its pins.
J3 is a HGB signal interface; see Table 4-21 for the distribution and definition of its pins.
4-25
Hardware System
J4 is a RBC/PLT signal interface; see Table 4-22 for the distribution and definition of its pins.
J5 is an interface to the main board; see Table 4-23 for the distribution and definition of its pins.
4-26
Hardware System
J6 is a power interface; see Table 4-24 for the distribution and definition of its pins.
J7 is a JTAG debugging interface; see Table 4-25 for the distribution and definition of its pins.
J8 is an interface to the volumetric board; see Table 4-26 for the distribution and definition of its
pins.
4-27
Hardware System
Assembly Drawing
See Figure 4-9 for the assembly drawing of the signal processing board.
4-28
Hardware System
Indicator Function
D11 3.3V power supply indicator
D14 5V power supply indicator
D15 12V power supply indicator
D22 FPGA configuration completion indicator
D23 Communication status indicator
D24 WBC sampling control indicator
D25 RBC sampling control indicator
4-29
Hardware System
Purpose
The signal processing board is a critical part in the whole device. When board failure occurs
due to various reasons, the board needs to be replaced and repaired in time to ensure the
normal operation of the device. Please replace the signal processing board according to below
procedure.
Tool
102 cross-headed screwdriver
Pliers (spare)
4-30
Hardware System
Removal
To avoid damaging the board and other parts, follow this procedure to remove it:
1. Power off the analyzer and ensure power connection of all boards are off
2. Disconnect the cable from the J6 socket as per Figure 4-8 Interface layout of the
signal processing board
3. Remove the upper shielding cover using the screwdriver as per Figure 4-12
Removing the signal processing board-2
4. Unplug cables successively from the J8, J5, J2, J1, J3 and J4 sockets as per Figure
4-8
5. Loosen the 6 screws on the board using the screwdriver as per Figure 4-11 (remove
the lower shielding cover only when necessary)
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
Installation
Follow this procedure strictly to install the signal processing board to the analyzer:
1. Install the lower shielding cover to the signal processing board as per Figure 4-10.
2. Fix the board to the analyzer using screws as per Figure 4-11.
3. Fix the upper shielding cover to the signal processing board as per Figure 4-12.
4. Connect other plugs/sockets to the signal processing board as per Figure 4-8.
Note: Before fixing the upper shielding cover, connect the RBC signal cable to the RBC socket
(J4).
Verification
After installation, power on the analyzer and check the signal processing board to see whether
the power indicator illuminates and the status indicator flashes normally. If yes, the verification
is passed (see Table 4-27).
4-31
Hardware System
Figure 4-10 Removing the lower shielding cover from the signal processing board
4-32
Hardware System
4-33
Hardware System
4.2.5 Troubleshooting
Error Message Judging Criteria Cause and Solution
Measured value exceeds The 3.3v circuit is not jointed properly / The
3.3v power
the normal range earth impedance decreases / The power IC
supply failure
(3.2v-3.4v) and related A/D converter go wrong.
Measured value exceeds The +5v circuit is not jointed properly / The
+5v power
the normal range earth impedance decreases / The power IC
supply failure
(4.85-5.25v) and related A/D converter go wrong.
Measured value exceeds The -5v circuit is not jointed properly / The
-5v power
the normal range earth impedance decreases / The power IC
supply failure
(-4.85-5.25v) and related A/D converter go wrong.
+12v power Measured value exceeds The +12v circuit is not jointed properly / The
supply failure the normal range earth impedance decreases / The power IC
4-34
Hardware System
4-35
Hardware System
Controlling DC motors
Controlling temperature
See Figure 4-13 for the block diagram of the driver board:
4-36
Hardware System
Description
Serial communication
The driver board consists of 6 MCUs (as shown in Figure 4-13) which include the mid
control unit (main MCU in Figure 4-13), sample transport unit, mix & pierce unit,
syringe unit, temperature control unit and other units.
The driver board communicates with the main board (UART) through the mid control
unit, receiving control instructions from and sending response instructions to the
main board. Its communication parameters are:
The mid control unit communicates with other 5 MCUs through the extended serial
ports of FPGA (EP1C3T144C8), sending control instructions to and receiving
response instructions from other MCUs. Its communication parameters are:
Controlling DC motors
The driver board has 2 DC motor circuits to control the mixing in the DIFF and BASO baths.
The MCU sends control signals to the MCU on the sample testing board, which then controls
the DC motor drive chip to start/stop the motors. The photocoupler detects the pulse signals of
motor speed and sends it to the MCU of the drive board through sample testing board. The
MCU then calculates the rotation speed of the motors.
4-37
Hardware System
The driver board controls 96 pumps and valves. The mid control unit sends the control signals
of SV1-SV88 to the pump/valve buffer of FPGA. As the data in the valve/pump buffer changes,
the FPGA outputs the valve/pump signals to the deserialize chip 74HCT595 to control the drive
chip IRLML2502 of valves and pumps, thus realizing the opening and closing of the valves and
pumps. The control signals of SSV89-SV96 are passed to the deserialize chip 74HCT595 by
the sample transport unit, the drive circuit is the same as SV1-SV88.
Controlling temperature
The built-in scanner scans the barcodes of tubes and tube racks. The driver board controls the
built-in scanner via FPGA. FPGA communicates with the scanners (UART) via the extended
serial port.
The pneumatic unit provides air force for the main unit and is driven by the pneumatic control
board. The driver board sends only the control signals of relay, which are converted and sent
to the pneumatic control board through the interface board
Lamp control circuit controls the laser on/off, monitors the feedback voltage and adjusts the
digital pot of the laser control board. The laser on/off signals are converted and sent to the
laser control board. The feedback voltage signals are amplifies and passed to the driver board,
which converts the signals to digital signals and then sent them to the MCU for calculation. The
driver board communicates with the laser control board via the SPI interface to control its
digital pot. The driver board sends data to the laser control board without getting response
from it.
The control signals of buzzer and indicators coming from the FPGA are converted and sent to
the indicator board to control the buzzer and indicators.
The driver board detects system temperature, pressure and voltage; the main board sends
inquiry command to obtain corresponding system status parameters.
Temperature detection refers to the detection of ambient temperature and laser diode
temperature. The sensors of ambient temperature and laser diode temperature are mounted
on the interface board and laser control board respectively. The temperature signals coming
from the sensors are sent to the A/D converter after I/V conversion and amplification. The
digital signals are then passed to the MCU, which calculates the temperature value based on
the signals.
The pressure sensor is mounted on the pressure detection board. 5 pressure circuits measure
the pressure of the vacuum chamber (2 channels), pressure chamber (2 channels) and sheath
fluid (1 channel) respectively. The pressure signals coming from the pressure sensors are
4-38
Hardware System
The driver board monitors the reagent status through the passive float switch with 15
monitoring channels.
Interface Definition
There are 36 interfaces on the driver board. See Figure 4-14 for their respective positions.
4-39
Hardware System
board
J4-J8 34 /
Connected to valves
J15,J47 20 /
Communication debugging
J12 4
interface Disabled
J14 5VDCoutput interface 2
Connected to the indicator /
J10 6
board
Connected to sample /
J11 8
transport motors
Connected to sample /
J13 20
transport sensors
Connected to sample mix /
J17 4
motors
Connected to wipe block /
J18 4
motors
Pressure detection /
J19 8
interface
Connected to mix & pierce /
J20 24
sensors
Connected to syringes, mix /
J21 20
motors and SRV sensors
Connected to the whole /
J22 4
blood aspiration motor
Connected to the WBC /
J24 4
injection syringe motor
Connected to sensors /
detecting the front door,
J27 12
open vial sampling and
wipe blocks
Connected to the /
J28 4
pneumatic control board
Connected to sensors /
J29 detecting the reagent and 20
laser box status
J30 Laser control interface 12 /
Connected to the mix /
J31 4
motors
Connected to the ambient /
J32 3
temperature sensor
Connected to the laser /
J33 3
diode temperature sensor
J35 Connected to the heater of 6 /
4-40
Hardware System
J2 is a power interface; see Table 4-31 for the distribution and definition of its pins.
J3 is an interface to the main board. See Table 4-32 for the distribution and definition of its
pins.
4-41
Hardware System
3 GND GND
PSEN signals of the mid
4 PC_PSEN
control unit
Reset signals of the mid
5 PC_RESET
control unit
J4, J5, J6, J7, J8 and J47 are interfaces to pumps and valves. See Table 4-33-Table 4-38 for
the distribution and definition of its pins
4-42
Hardware System
21 22 SV26 Valve26
23 24 SV27 Valve27
25 26 SV28 Valve28
27 28 SV29 Valve29
29 30 SV30 Valve30
31 32 SV31 Valve31
33 34 SV32 Valve32
1 2 SV33 Valve33
12V 12V power supply
3 4 SV34 Valve34
5 NC No contact 6 NC No contact
7 8 SV35 Valve35
9 10 SV36 Valve36
11 12 SV37 Valve37
13 14 SV38 Valve38
15 16 SV39 Valve39
17 18 SV40 Valve40
19 20 SV41 Valve41
12V 12V power supply
21 22 SV42 Valve42
23 24 SV43 Valve43
25 26 SV44 Valve44
27 28 SV45 Valve45
29 30 SV46 Valve46
31 32 SV47 Valve47
33 34 SV48 Valve48
4-43
Hardware System
19 20 SV57 Valve57
21 22 SV58 Valve58
23 24 SV59 Valve59
25 26 SV60 Valve60
27 28 SV61 Valve61
29 30 SV62 Valve62
31 32 SV63 Valve63
33 34 SV64 Valve64
4-44
Hardware System
19 NC No contact 20 NC No contact
J10 is an interface to the indicator board. See Table 4-39 for the distribution and definition of its
pins.
J11 is the interface to the motors of sample transport unit. See Table 4-40 for the distribution
and definition of its pins.
4-45
Hardware System
J13 is the interface to the sensors in the sample transport unit (connecting to the sensors via
an adapter). See Table 4-41 for the distribution and definition of its pins.
4-46
Hardware System
J14 is the output interface of 5V DC power supply and is disabled on the analyzer.
J15 is an interface connecting pumps and valves (mix & pierce unit). See Table 4-42 for the
distribution and definition of its pins.
Table 4-42 Definition of the interface connecting pumps and valves (mix & pierce unit)
4-47
Hardware System
5 6 SV91 Valve91
7 8 SV92 Valve92
9 10 SV93 Valve93
11 12 SV94 Valve94
13 14 SV95 Valve95
15 16 SV96 Valve96
17 NC No contact 18 NC No contact
19 NC No contact 20 NC No contact
J17 is the interface to the motors of sample mix unit. See Table 4-43 for the distribution and
definition of its pins.
J18 is an interface connecting wipe block motors. See Table 4-44 for the distribution and
definition of its pins.
J9 is a pressure detection interface; see Table 4-45 for the distribution and definition of its pins.
4-48
Hardware System
160KPapressure detection
2 PRESSURE_2ST
signal
700KPapressure detection
3 PRESSURE_3ST
signal
-40KPapressure detection
4 PRESSURE_4ST
signal
-85KPapressure detection
5 PRESSURE_5ST
signal
Reserved channel
6 PRESSURE_6ST
pressure detection
7 AVCC 5V analog power supply
8 AGND Analog ground
J20 is an interface connecting sensors (mix & pierce unit). See Table 4-46 for the distribution
and definition of its pins.
Table 4-46 Definition of the interface connecting sensors (mix & pierce unit)
4-49
Hardware System
J22 is the interface to the motor of the whole blood aspiration syringe. See Table 4-48 for the
distribution and definition of its pins.
Table 4-48 Definition of the interface connecting to the whole blood aspiration motor
J24 is the interface to the motor of WBC sample injection syringe. See Table 4-49 for the
distribution and definition of its pins.
4-50
Hardware System
Table 4-49 Definition of the interface connecting the WBC sample injection syringe
motor
J27 is a sensor interface; see Table 4-50 for the distribution and definition of its pins.
J28 is the interface connecting the pneumatic control board; see Table 4-51 for the distribution
and definition of its pins.
4-51
Hardware System
J29 is a reagent sensor interface; see Table 4-52 for the distribution and definition of its pins.
J30 is a laser control interface. See Table 4-53 for the distribution and definition of its pins.
4-52
Hardware System
12 NC No contact
J31 is an interface connecting DC motors. See Table 4-54 for the distribution and definition of
its pins.
J32 and J33 are temperature sensor interfaces, see Table 4-55 and Table 4-56 for the
distribution and definition of their pins.
Table 4-56 Definition of J33- interface of the laser box temperature sensor
J35 is the interface connecting the heater of the preheating bath; see Table 4-57 for the
distribution and definition of its pins.
Table 4-57 Definition of the interface connecting the heater of preheating bath
4-53
Hardware System
J36 is the interface connecting the heater of the reaction bath; see Table 4-58 for the
distribution and definition of its pins.
Table 4-58 Definition of the interface connecting the heater of the reaction bath
J37 is a laser diode heater interface; see Table 4-59 for the distribution and definition of its
pins.
J38 is the internal reagent interface. See Table 4-60 for the distribution and definition of its
pins.
4-54
Hardware System
J43 is the interface connecting fans of the main unit. See Table 4-61 for the distribution and
definition of its pins.
Table 4-61 Definition of the interface connecting fans of the main unit
J44 is a power interface; see Table 4-62 for the distribution and definition of its pins.
J48 is the electromagnet interface; see Table 4-63 for the distribution and definition of its pins.
Assembly Drawing
The assembly drawing of the driver board is shown in Figure 4-16.
4-55
Hardware System
4-56
Hardware System
Indicator Description
D1 FPGA configuration status indicator, illuminates during FPGA configuration
D2 Mid control unit status indicator, flashes every 0.5s when the unit runs
normally
D3 Sample transport unit status indicator, flashes every 0.5s when the unit runs
normally
D12 FPGA status indicator, flashes every 0.5s when the FPGA runs normally
D13 Syringe unit status indicator, flashes every 0.5s when the unit runs normally
D14 Other units status indicator, flashes every 0.5s when the units runs normally
D16 Temperature control unit status indicator, flashes every 0.5s when the unit
runs normally
D17 5V analog power supply indicator, illuminates when the power supply is
normal.
D21 Indicates the working status of the preheating heater. It turns on during
heating, turns off when heating is stopped, and flashes during temperature
tuning.
D22 Indicates the working status of the reaction bath heater. It turns on during
heating, turns off when heating is stopped, and flashes during temperature
tuning.
D23 Indicates the working status of the laser diode heater.
D24 5V digital power supply indicator, illuminates when the power supply is
normal;
D25 12V power supply indicator, illuminates when the power supply is normal
D26 3.3V power supply indicator, illuminates when the power supply is normal
D27 24V power supply indicator, illuminates when the power supply is normal
4-57
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4-58
Hardware System
4-59
Hardware System
Key Definition
The reset switch S1 on the driver board is for manually resetting the driver board. Pressing the
reset switch can reset all MCUs and the FPGA.
Tool
107cross-headed screwdriver
Removal
Turn off the analyzer and disconnect all power cords;
Remove the left door and top cover (See Section 5.2.1 and Section 5.2.4 for the
detailed steps);
Use the PCB assembly stop bar to support the main board and signal processing
board assembly as per Figure 4-17;
Unplug all wires connected to the sockets of the driver board. See Table 4-66.
Loosen the 11 cross-recessed panhead screws -M3X6 with the screwdriver. See
Figure 4-17.
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
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Hardware System
Serial No. Socket label(on the driver board) Cable label(on cables)
1 J2 STANDBY C-J2/C41306
2 J3 CPU C-J3/C41288
4 J4 SV(01-16) C-J4/C000176
5 J5 SV(17-32) C-J5/C000177
6 J6 SV(33-48) C-J6/C41309
7 J7 SV(49-64) C-J7/C49000
8 J8 SV(65-80) C-J8/C000178
9 J10 INDICATION C-J10/C41305
10 J11 TTRF-MOTOR C-J11/C49020
11 J13 TTRF-SENSOR C-J13/C49020
12 J15 SV(89-96) C-J15/C49010
13 J17 M-ASR C-J17/C49006
14 J18 M-MS C-J18/C49007
15 J19 C-J19/C000440
16 J20 ASR-SENSOR C-J20/C49006
17 J21 SYRINGE-SENSOR C-J21/C49007
18 J22 M-SWB C-J22/C49007
19 J24 M-SWBC C-J24/C49007
20 J27 MS-SENSOR C-J27/ C49007
21 J28 AIR-CTRL C-J28/C49012
22 J29 REAGENT-SENSOR C-J29/C49027
23 J30 LASER C-J30/C49013
24 J31 DC-MOTOR C-J31/C49007
25 J32 TM1 C-J32/C49025
26 J34 TM3 C-J34/C49024
27 J35 HT-PRE C-J35/C49023
28 J36 HT-ACT C-J36/C49023
29 J38 REAGENT-INSIDE C-J38/C49027
30 J43 FAN C-J43/C41304
31 J44 POWER C-J44/C41303
32 J47 SV(81-88) C-J47/C49007
33 J48 DOOR C-J48/C41297
4-61
Hardware System
Installation
Install the driver board as the above-mentioned procedure in reverse order.
Verification
1. Check the driver board to ensure all connections are correct.
2. When the analyzer is off, connect the analyzer to the power supply and start it up.
3. Check whether the indicators D2, D3, D12, D13, D14 and D16 on the driver board
flash. You can also check the indicators by entering the self-test screen and
performing a self-test. If the self-test is passed, it indicates that the driver board is
replaced successfully; otherwise you must troubleshoot the board.
4.3.5 Troubleshooting
4-62
Hardware System
and service engineers. When an error is reported, the error No. and error name are provided
by host control software. The recommended action to remove the error may appear on the
corresponding screen as the software help information.
When checking an error, ensure the connection wires related to the error is properly connected.
For example, when "AL: load motor to start position failed" is reported, check whether the
connection between the sample transport assembly and the driver board is stable, whether the
plugs at ends are well connected (if improper connection is identified, shutdown the analyzer,
disconnect it from power supply, and then reconnect), whether the connection inside the
sample transport assembly is proper, whether the label on the sensor connecting wire
corresponds to the sensor position and whether the label on the motor connecting wire
corresponds to the motor position (disassemble the sample transport assembly to check, see
Chapter 5 Replacing the Sample Transmitting Assembly for the procedure).
After the improper connection possibility is excluded, remove errors according to the
recommended action in the following table. If an error remains after the cause is found and
components get replaced, you need to replace the driver board.
The following table shows the error codes, error names and the recommended actions to
remove errors.
4-63
Hardware System
Last priming and draining Fluidic sequence or host software error. Try
0x0000000B
haven't finished again or restart the analyzer.
1 Check whether there are enough
reagents;
Priming and draining do not 2 The float switch is damaged, replace
0x0000000E
finish in regular time it.
3 Disassemble the analyzer to check
for tubing leakage.
Host software error or communication
Transmit unit: function code
0x00000064 interference. Try again or restart the
error
analyzer.
Host software error or communication
Transmit unit: address code
0x00000065 interference. Try again or restart the
error
analyzer.
Host software error or communication
Transmit unit: data length
0x00000066 interference. Try again or restart the
error
analyzer.
Host software error or communication
Transmit unit: command ID
0x00000067 interference. Try again or restart the
error
analyzer.
Host software error or communication
Transmit unit: verification
0x00000068 interference. Try again or restart the
code error
analyzer.
Host software error or communication
0x00000069 Transmit unit: end code error interference. Try again or restart the
analyzer.
The autoloader loading start position
photocoupler is open-circuited. Possible
phenomena are: the autoloader loading
motor is at the start position or the
AL: load motor to start
0x0000006A autoloader loading motor hits the limit
position failed
point.
Autoloader loading motor failure. The
phenomenon is the autoloader load motor
does not move.
The autoloader load stop position
photocoupler or the contact switch is
open-circuited. Possible phenomena are:
the autoloader loading motor is at the stop
AL: load motor to start
0x0000006B position or the autoloader loading motor
position failed
hits the limit point.
Autoloader loading motor failure. The
phenomenon is the autoloader load motor
does not move.
4-64
Hardware System
4-65
Hardware System
4-66
Hardware System
4-67
Hardware System
4-68
Hardware System
4-69
Hardware System
4-70
Hardware System
analyzer.
Host software error or communication
Temp. unit: address code
0x00000191 interference. Try again or restart the
error
analyzer.
Host software error or communication
0x00000192 Temp. unit: data length error interference. Try again or restart the
analyzer.
Host software error or communication
Temp. unit: command ID
0x00000193 interference. Try again or restart the
error
analyzer.
Host software error or communication
Temp. unit: verification code
0x00000194 interference. Try again or restart the
error
analyzer.
Host software error or communication
0x00000195 Temp. unit: end code error interference. Try again or restart the
analyzer.
1 If D25 is not on, check the 12VDC power
supply;
2 If the D25 is on, check whether the beads
in the AD circuit module or other
components are not welded completely;
0x00000196 PDB: ADS1210 error
3 Check whether the crystal oscillator X2 is
short-circuited.
4 If none of the above problems is found,
ADS1210 is damaged. Please replace the
driver board.
1 Check whether the preheat temperature
sensor is well connected;
Heater: preheat bath temp. 2 Check whether the +12V power supply
0x00000197
sensor error on the driver board works normally;
3 If error remains when the sensor is
connected, the sensor is damaged.
1 Check whether the reaction temperature
sensor is well connected;
Heater: reaction bath temp. 2 Check whether the +12V power supply
0x00000198
sensor error on the driver board works normally;
3 If error remains when the sensor is
connected, the sensor is damaged.
1 Check whether the laser diode
temperature sensor is well connected;
Laser diode temp. sensor 2 Check whether the +12V power supply
0x00000199
error on the driver board works normally;
3 If no problem is found in the above
procedure and the error remains, the
4-71
Hardware System
sensor is damaged.
1 Check whether any component in the
EEPROM circuit is not welded, such as L7
and R3; check whether the pins of
EEPROM are short-circuited or welded
0x0000019B PDB: EEPROM error
incorrectly.
2 If none of problems above is found and
the error remains, the EEPROM is
damaged.
Host software error or communication
Other unit: function code
0x000001F4 interference. Try again or restart the
error
analyzer.
Host software error or communication
Other unit: address code
0x000001F5 interference. Try again or restart the
error
analyzer.
Host software error or communication
0x000001F6 Other unit: data length error interference. Try again or restart the
analyzer.
Host software error or communication
0x000001F7 Other unit: command ID error interference. Try again or restart the
analyzer.
Host software error or communication
Other unit: verification code
0x000001F8 interference. Try again or restart the
error
analyzer.
Host software error or communication
0x000001F9 Other unit: end code error interference. Try again or restart the
analyzer.
1 Check whether the +12V power supply of
the driver board is normal;
2 Check whether the voltage of PIN5
0x000001FA PDB: U74(AD7928) error (AVDD) on U74 is normal. The normal
voltage is +5V5% ;
3 If neither problem is found, U74
(AD7928) is damaged.
1 Check whether the +12V power supply of
the driver board is normal;
2 Check whether the voltage of PIN5
0x000001FB PDB: U75(AD7928) error (AVDD) on U75 is normal. The normal
voltage is +5V5%;
3 If neither problem is found, U75
(AD7928) is damaged.
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Hardware System
"AL: feeding unit counter error" is reported when feeding motor feeds twice consecutively yet
the readings of the left and right counter of the feeding unit do not change. When this error is
reported, troubleshoot it according to the steps that follow.
1. Check whether tube racks on the loader tray enter the feeding flow line after they are
loaded in position. Some racks may not enter the flow line since they are so long that
the claw can not get them. In case of this, install racks of appropriate size and start
the autoloading flow again according to the prompt on the software screen.
2. Check whether tube racks get blocked in the feeding flow line. This may be reported if
autoloader feeding motor cannot push the racks due to distorted racks or wrongly
assembled sample transport assembly. In case of this, turn off the analyzer,
disconnect all the power cords and pull the racks manually on the flow line to judge
the possible causes. If it results from distorted racks, install appropriate racks. If it
results from wrongly assembled assembly, remove and repair the assembly.
3. Check whether tube racks get blocked and the autoloader feeding motor cannot push
the racks due to other racks tilted on the unloader tray or improper tray manufacture.
Check whether the rack length is qualified and whether the sheet-metal near the
unloader unit is correctly fabricated.
4. If the current rack is not unloaded and the unloader assembly stops the movement
while previous racks were moved to the unloader tray and the tray is not full, do as the
prompt on the software screen: stop the autoloader, remove all racks on the unloader
tray, place a rack on the loader tray and then start the autoloader. If this is successful,
the cause is the same with that listed in step 3. If this error is still reported, turn of the
analyzer, remove the sample transport assembly, replace the left and right
photocouplers in the autoloader feeding position and check whether the baffles can
work normally.
5. If the AL: feeding unit counter error cannot be removed by the process above,
the error occurs due to the failure of driver board components. Replace the driver
board.
1. If all vacuum and pressure of the system are zero, check the connection between the
pressure board and the driver board/pneumatic tubing, and whether the pneumatic
unit starts normally;
2. If all vacuum and pressure of the system are abnormal and with the too high or too
low pressure value at the same time, check whether the air compressor can work
normally. See Chapter XX for a reference of the troubleshooting method;
3. If only one pressure value is abnormal, check the related regulator and airway for
leakage.
4. If the airway system is normal, the error is due to the pressure sensor failure in the
driver board. Replace the driver board.
5. See the following table for the output voltage and pressure of the pressure sensor:
4-73
Hardware System
For the abnormal actions of system mechanism, service engineer needs to observe and
troubleshoot them. In case of these errors, troubleshoot them according to the recommended
actions listed in the table above and the procedure below.
1. Check whether the related units (such as motors and cylinders) can move. If the units
do not move, the error is due to the executive component problems, such as
disconnected motor, airway leakage or the failure of corresponding air valve to
cylinder. If the errors mentioned above are excluded, the errors occurs due to the
driver board failure. Replace the driver board.
2. If the units can move, check whether the movement is in position and whether units
are blocked by lines or other units during the movement. For transmissive
photocoupler, if the baffle enters 2/3 or above of the photocoupler, it is baffled and the
movement is in position.
3. For the cylinder movement, check whether the movement is too slow. Abnormal
0.25MPa pressure or oversized throttle in the airway connecting the cylinder may
lead to abnormal movement of cylinder and cause failure.
4. If errors remain after unit movement problems are excluded, consider photocoupler
failure. Replace the photocoupler.
If errors still exist after the photocoupler is replaced, the cause is failure of components on the
driver board. Replace the driver board.
4-74
Hardware System
Function
The 3100 power board provides stable and reliable DC power source all the functional blocks
of the BC-5800 hematology analyzer and for the equipment itself, including 5V, 12V and 24V.
See below Figure 4-18 for the functional block diagram of the power board.
AC input PFC
EMI circuit and Power board
circuit 051-000159-00
rectifier circuit
Vcc 390V
5B Standby VDD
Active clamp 24V DC/DC 12V
circuit normal wave 12V circuit output
circuit
On/off
signals
Description
The power board operates under 90V-264V AC input voltage (50/60Hz).
When the AC power switch is ON, only the standby circuit works. The 24V, 12V and 5V are
output only when the power button is ON.
Circuit loads of the power board are described in Table 4-68 below:
4-75
Hardware System
Interface Definition
The power board has 6 external interfaces numbered from J1-J18. The positions of all
interfaces on the board are portrayed as below Figure 4-19.
4-76
Hardware System
J1 is the AC input interface; see Table 4-70 for the distribution and definition of its pins.
J2 is the output power interface of the main board; see Table 4-71 for the distribution and
definition of its pins.
J3 is the output power interface of the signal processing board; see Table 4-72 for the
distribution and definition of its pins.
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Hardware System
Second
3 5V 5V power output 4 SGND
ground
12V power Second
5 12V 6 SGND
output ground
J4 is the output power interface of the driver board; see Table 4-73 for the distribution and
definition of its pins.
J5 is the signal control interface of the power button. See Table 4-74 for the distribution and
definition of its pins.
J6 is the 12V fan power interface of the power box; see Table 4-75 for the distribution and
definition of its pins.
Table 4-75 Definitions of the 12V fan power interface of the power box
4-78
Hardware System
source
Assembly Drawing
4-79
Hardware System
Indicator Description
LED1 5B indicator
LED2 5V indicator
LED3 12V indicator
LED4 24V indicator
4-80
Hardware System
waveform of Q301
16 TP16 Q301.1 Measures the driving /
waveform of Q301
17 TP17 Q302.2 Measures the switching /
waveform of Q302
18 TP18 Q302.1 Measures the driving /
waveform of Q302
19 TP19 Q302.3 Measures the current /
waveform of Q302
20 TP20 C324.+ Measures the output 241V
voltage of 24V1
21 TP21 D305.1 Measures the voltage /
waveform of the rectifier
and the fly-wheel diode
22 TP22 U301.4 Measures the reference 5V
voltage of U301
23 TP23 Q401.1 Measures the driving /
waveform of Q401
24 TP24 Q402.2 Measures the switching /
waveform of Q402
25 TP25 Q402.1 Measures the driving /
waveform of Q402
26 TP26 C412.+ Measures the output 120.5V
voltage and ripple voltage
of 12V1
27 TP27 U401.11 Measures the 10V 10V
reference voltage of U401
28 TP28 Q411.1 Measures the driving /
waveform of Q411
29 TP29 Q412.2 Measures the switching /
waveform of Q412
30 TP30 Q412.1 Measures the driving
waveform of Q412
31 TP31 C442.+ Measures the output 50.5V
voltage and ripple voltage
of 5V1
32 TP32 U411.11 Measures the 10V 10V
reference voltage of U411
4-81
Hardware System
various reasons, the board needs to be replaced and repaired in time to ensure normal
operation of the device. Please replace the power board according to below procedure.
Tool
107 cross-headed screwdriver
Multimeter
Removal
1. Turn off the analyzer and disconnect AC power cords;
2. Remove all connections from the power output terminal. Then refer to Section 6.3 of
Chapter 6 to remove the fixing screws to take out the power assembly from the main
unit enclosure;
3. Refer to Section 6.3 of Chapter 6 to dismantle the power box. Unplug the AC
connection cables on the power board. Then remove the nine fixing screws on the
power board to take out the power board from the power box.
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
Installation
Install the driver board as the above-mentioned procedure in reverse order.
Verification
1. Verify whether the ground wire of AC input socket is well connected to the main unit
enclosure (the conductive part);
2. Connect power cord, switch on the AC control switch, the 5B indicator on the power
board illuminates; switch on the power button, the power fans start to run.
z During installation, ensure that the AC plug and fan plug on the power box
are connected to the power board, and then fix the upper cover of the power
box.
z Ensure that the power assembly and the main unit enclosure are firmly
connected with screws.
4-82
Hardware System
4.4.5 Troubleshooting
Error analysis and processing
Errors of the power board can be checked out according to the procedure in Figure 4-21.
Ensure that the power assembly and the main unit enclosure are firmly connected with screws.
4-83
Hardware System
Function
The interface board provides electricity connection between the hematology analyzer and the
external equipments.
Description
The interface board provides the following interfaces for the external equipments:
J2: network interface (RJ45);
J4: pneumatic interface;
J11: serial port (RS232)
J6-J9: 4-channel USB interface
Interface Definition
The interface board has 11 external interfaces numbered from J1-J11.The positions of all
interfaces on the board are portrayed as below Figure 4-22.
4-84
Hardware System
Number
Interface Function Description
of pins
Network interface (connected to the /
J1 6
main board)
Network interface RJ45 (connected to /
J2 8
the external equipments)
Pneumatic control input interface /
J3 4
(connected to the driver board)
Pneumatic control output interface /
J4 9
(connected to the pneumatic board)
J5 USB data wire interface 20 /
USB interface Standard USB
J6 4
interface
USB interface Standard USB
J7 4
interface
USB interface Standard USB
J8 4
interface
USB interface Standard USB
J9 4
interface
External scanner interface RS232 electrical
J10 6
(connected to the main board) level
J11 External scanner interface 9 /
4-85
Hardware System
J1 is a network interface of the main board; see Table 4-79 for the distribution and definition of
its pins.
J2 is a network interface of the main board; see Table 4-80 for the distribution and definition of
its pins.
J3 is a pneumatic control interface. See Table 4-81 for the distribution and definition of its pins.
4-86
Hardware System
J4 is a pneumatic control output interface. See Table 4-82 for the distribution and definition of
its pins.
J5 is an USB interface of the main board, see Table 4-83 for the distribution and definition of its
pins.
4-87
Hardware System
18 GND /
19 DM1 USB1 data signals (difference signals)
20 DP1 USB1 data signals (difference signals)
J6-J9 are standard USB interfaces, see Table 4-84 for the distribution and definition of its pins.
J10 is the external scanner interface connecting with the main board. It is not used presently.
J11 is the external scanner interface connecting with the scanner. It is not used presently.
Assembly Drawing
See Figure 4-23 for the assembly drawing of the interface board
4-88
Hardware System
Removal
1. Turn off the analyzer and disconnect all power cords.
3. Remove the left door (Fro details, refer to Section 6.2.1 of Chapter 6).
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
Installation
Install the interface board as the above-mentioned procedure in reverse order.
Verification
1. Connect the power cord. Switch on the power switch to start the hematology analyzer.
2. Insert a USB mouse to any of the interfaces on the interface board, move the mouse
to see if cursor is moving on the display. If yes, the interface board is working normally;
if no, please check the connection between the interface board and the USB signal
wire of the main board.
4-89
Hardware System
Description
Indicating the operating status of the main unit
The indicator board indicates the operating status of the equipment through a yellow/green
indicator and a buzzer. When the system is in standby status, the indicator is turns yellow;
when the system works normally, the indicator turns green; When error occurs to the system,
the indicator is green and flashes at 1Hz frequency. When the system works normally, the
buzzer does not make a sound; when error occurs to the system, the buzzer buzzes.
Power on: when the power cord is connected and the power switch is ON, the system is in
standby status (the yellow/green indicator turns yellow), the power system is OFF. When the
power button on the indicator board is pressed, the power system is ON. The equipment starts
to operate and the indicator turns green.
Interface Definition
The indicator board is configured with only one external interface. Its definition is as follows.
J1 is the interface of the indicator board. See Table 4-85 for the distribution and definition of its
pins.
4-90
Hardware System
Assembly Drawing
See Figure 4-24 for the assembly drawing of the indicator board
2. Open the upper part of the front cover and fix it with the stop bar.
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
Installation
Install the interface board as the above-mentioned procedure in reverse order.
4-91
Hardware System
Verification
Connect the power cord. Switch on the power switch. The yellow/green indicator should turn
yellow.
Press down the power button to start the system. The indicator should turn green.
Troubleshooting
Error analysis and processing
Indicator failure
Description: When the system is in standby or normal working status, the yellow/green
indicator does not illuminate.
Possible cause: the connection wire on the indicator board is not in good contact with the
indicator socket J1. Or the indicator is damaged.
Solution:
1. Check whether the connection wire on the indicator board is reliably connected with
J1. If not, disconnect the wire and re-plug it into J1.
2. Re-power on the system. Observe whether the indicator is on. If yes, the problem is
solved. If not, replace the indicator board.
4-92
Hardware System
Description
The touchscreen control board is connected to the 4-line touchscreen through J4. It provides
voltage to the electrode of the touchscreen and receives information of touch points;
The board is connected to the main board through J5. It sends information of touch points to
the main board through RS232 level serial port.
Interface Definition
The touchscreen control board is configured with three external interfaces.
J1 is the interface configured for CPLD on the touchscreen control board; see Table 4-86 for
the distribution and definition of its pins.
4-93
Hardware System
Table 4-86 Definitions of the interface configured for CPLD on the touchscreen control
board
J4 is the interface for the touch screen; see Table 4-87 for the distribution and definition of its
pins.
J5 is the interface of the main board; see Table 4-88 for the distribution and definition of its
pins.
Assembly Drawing
The assembly drawing of the touchscreen control board is shown in Figure 4-26.
4-94
Hardware System
(a) Top
(b) Bottom
Table 4-89 Adjustment and test points on the touchscreen control board
4-95
Hardware System
Tool
107 cross-headed screwdriver
Removal
1. Switch off the hematology analyzer and unplug the power cord.
2. Open the upper part of the front cover (for details, see Section 5.2.3 of Chapter 5).
4. Use the screwdriver to loosen the two cross-recessed panhead screws (with lock
washer) on the fixing plate of the touchscreen controller and remove the fixing plate.
5. Use the screwdriver to loosen the two M3x6 cross-recessed panhead screws (with
lock washer) on the touchscreen control board.
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
Installation
Install the board per the above-mentioned procedure in reverse order.
Verification
1. Connect the power cord. Switch on the power switch to start the hematology analyzer.
2. Press the touchscreen to see whether the cursor moves with the finger.
4.7.5 Troubleshooting
Table 4-90 Error analysis and processing of the touchscreen control board
4-96
Hardware System
4-97
Hardware System
4.8.3 Description
The power board operates under 90V-264V AC input voltage (50/60Hz).
When the control signal is low level and the output power is 12V, the indicator illuminates, the
fan works, the relay is closed, and the pneumatic unit starts running.
4-98
Hardware System
J1 is the switch control interface. See Table 4-92 for the distribution and definition of its pins.
J2 is the indicator output interface. See Table 4-93 for the distribution and definition of its pins.
J3 is the fan output interface. See Table 4-94 for the distribution and definition of its pins.
4-99
Hardware System
4 12V0 O Fan 2+
5 GND O Fan 2-
J4 is the AC input interface, see Table 4-95 for the distribution and definition of its pins.
J5 is the output interface of the pneumatic compensating capacitor; see Table 4-96 for the
distribution and definition of its pins.
J6 is the output interface of the temperature protection switch. See Table 4-97 for the
distribution and definition of its pins.
4-100
Hardware System
Assembly Drawing
Tool
107 cross-headed screwdriver
Removal
1. Switch off the pneumatic unit and unplug the AC power cord.
4-101
Hardware System
2. Remove the pneumatic cover, remove all plug-ins from the pneumatic control board,
loosen the screws fixing the board, and then remove the board from the pneumatic
unit.
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
Installation
Install the board per the above-mentioned procedure in reverse order.
Verification
1. Connect the power cord and switch on the AC control switch.
2. Enter the maintenance screen. When the pneumatic unit is turned on, the green
indicator lights on, the fan works, and the pneumatic unit starts working (with
vibration).
4.8.6 Troubleshooting
Error analysis and processing
You can service the pneumatic control board taking it as a single-end backward circuit. The
parts to be considered include protective tube, switch tube Q4, sampling resistor R18 and
control chip U1. In addition, when servicing the board, you should short circuit the two pins of
J6 and connect them to the power supply and then test the board.
4-102
Hardware System
z Care must be taken to avoid electric shock when servicing the pneumatic
control board.
The pre-amplification board consists of low angle board (LAS) and high angle board (MAS).
The LAS board converts the forward light signal to electric signal, and then amplifies it. The
MAS board converts the lateral light signal to electric signal, and then amplifies it. There is
positive / negative voltage converting circuit on the pre-amplification board.
The laser control board mainly drives the laser semiconductor. It is required that the laser
output is between 3mW and 5mW, the laser power is stable, and the intensity is adjustable.
Volumetric board
The volumetric board determines the measuring time for RBC/PLT count. That is, the board
provides the signals of count starting and count ending.
Description
Refer to Section 5.9.2 for function description of the three boards.
Interface Definition
The functions of pre-amplification board, laser control board and volumetric board are not so
extensive, so there are only a few interfaces on the boards. See Figure 4-31 - Figure 4-33 for
the position of the interfaces.
4-103
Hardware System
There is one interface between the pre-amplification board and the signal processing board, its
definition is shown in Table 4-98.
Table 4-98 The interface between the pre-amplification board and signal processing
board (J1)
4-104
Hardware System
The laser control board is configured with 2 interfaces. See Table 4-99 - Table 4-100 for the
detailed definitions.
Table 4-99 The interface between the laser control board and driver board
(J1--PWR-SIG)
Table 4-100 The interface between the laser control board and the laser semiconductor
(J2--LASER DIODE)
There is one interface J1 between the volumetric board and the signal processing board, its
definition is shown in Table 4-101.
Table 4-101 The interface between the volumetric board and signal processing board
(J1)
4-105
Hardware System
3 GND / Ground /
4 START O Count-starting signal TTL
5 STOP O Count-ending signal TTL
6 NVMBLED I On/Off control of constant-current TTL
source
Assembly Drawing
See Figure 4-34 for the assembly drawing of the pre-amplification board.
See Figure 4-35 for the assembly drawing of the laser control board.
4-106
Hardware System
See Figure 4-36 for the assembly drawing of the volumetric board.
There is no adjustable component; test points are shown in Table 4-102, and their positions
4-107
Hardware System
If the sliding variable resistor VR1 is adjusted clockwise, the resistance of VR1 in the circuit
increases, the voltage of the test point TP2VREF and the laser intensity also increases; if the
VR1 is adjusted counterclockwise, the resistance of VR1 in the circuit, the voltage of the test
point TP2VREF and the laser intensity all decreases.
The voltage of TP2VREF should be between 4.0 and 4.1V. Then the voltage at the point of
TPILD is between 0.8 and 2.0V (this happens only when the laser semiconductor is
connected).
Refer to Table 4-103 for test points and Figure 4-35 for positions.
4-108
Hardware System
Volumetric board
Under background condition (without volumetric tube), adjust VR4 to turn the voltage of TP2 to
2.55-2.65V. Then install volumetric board and check whether the voltage of TP2 is 2.90V.
Under background condition (without volumetric tube), adjust VR3 to turn the voltage of TP3 to
2.55-2.65V. Then install volumetric board and check whether the voltage of TP3 is 2.90V.
Refer to Table 4-104 for test points and Figure 4-36 for positions.
4-109
Hardware System
Tool
102 cross-headed screwdriver
Removal
The operation of removal is performed as per the reverse operation of installation described in
4.9.4 .
Installation
Pre-amplification board
Refer to Figure 4-37 and Figure 4-38 for the position of the pre-amplification board in the
analyzer and information of its assemblies. To ensure that the board is correctly installed in the
analyzer, please do strictly as instructed below:
1. 2 ) with the PD
Align the photo detector on the pre-amplification board (Figure 4-38,
1 ), then place the pre-amplification board onto the support, and
sheath (Figure 4-38,
4 ).
then secure the fixing screws (Figure 4-38,
2. Shield the pre-amplification board using the shielding cover (Figure 4-38,
3 ) and
secure the fixing screws (Figure 4-38, ) to finish the PD installation. The two steps
above apply to the high angle board and low angle board.
3. Place the high angle PD assembly and low angle PD assembly onto the optical
system support (Figure 4-37,
1 ) as Figure 4-37 and
2 shown in
3 ; secure the
fixing screws.
4-110
Hardware System
Refer to Figure 4-39 and Figure 4-40 for the position of the laser control board in the analyzer
and information of its assemblies. To ensure that the board is correctly installed in the analyzer,
please do strictly as instructed below:
1. According to the direction shown in Figure 4-40, place the laser control board (Figure
3 ) and then secure the fixing
4-40, ) onto the control board support (Figure 4-40,
screws (Figure 4-40, ).
2. After step 1, place the control board support onto the optical system support (Figure
4-39, ) and secure the fixing screws (Figure 4-39, ).
4-111
Hardware System
Volumetric board
Refer to Figure 4-41 for the position of the volumetric board in the analyzer and information of
its assemblies. To ensure that the board is correctly installed in the analyzer, please do strictly
as instructed below:
it in place.
4-112
Hardware System
3. Put the assemblies of step (1) on the volumetric board, and adjust the positioning
holes as well. That is, the four positioning holes of the enclosure, volumetric board
and support shall coincide. Fix them with screws (Figure 4-41,
5 ).
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
Verification
Pre-amplification board
1. Install the pre-amplification board in the analyzer correctly (Remember: cover the
laser assembly with the shielding box).
2. Perform a standard sample analysis and then check the scattergram. Normal
scattergram indicates that you have replaced the pre-amplification board successfully.
4-113
Hardware System
3. Install the laser control board in the analyzer by fixing the screws and connecting the
laser tube and temperature sensor to the board properly. Start up the analyzer.
Volumetric board
1. Install the volumetric board in the analyzer by fixing the screws and connecting cables
to the board properly. Start up the analyzer.
2. Perform a sample analysis and check the status of the indicators on the volumetric
board. When the metering tube is empty, the indicators are off; when the liquid in the
metering tube passes through the photocoupler, the corresponding indicator
illuminate (OP1 to D1, OP2 to D2).
4-114
Hardware System
4.9.5 Troubleshooting
4-115
Hardware System
4-116
Hardware System
Table 4-106 Error analysis and processing of the laser control board
4-117
Hardware System
4-118
Hardware System
Volumetric board
4-119
Hardware System
Error of The output voltage Something is wrong with the 5V power. Low See No.2 above.
Board
3 comparator of TP1 is not
error Chip U4 is of dry joint or erroneous. Low Replace or re-joint U4.
reference power 2.5V0.05V
The connection cable with the signal board is
The voltage of Medium Replug or replace the cable.
improperly connected or the cable is broken.
Control signal System NVMBLED signal
4
error error is no less than The control signal from the signal processing Refer to the troubleshooting
Low
0.8V. board is erroneous. section of the signal board.
4-120
Hardware System
through the
volumetric tube; or
the indicator is on The voltage at test point TP2 or TP3 is not
Medium See No.6 above.
although there is 2.55-2.65V.
no liquid in the
volumetric tube.
4-121
Hardware System
Description
For motors and scanners, only the corresponding sockets are provided for the electricity
connection of motors/scanners.
There are 10 sensors in the sample transport unit, which include 7 position detection
photocouplers, 1 position detection sensitive switch, 1 tube detection photocoupler and 1
reserved sample detection photocoupler. The sample transport board drives the 10 sensor,
receives and amplifies signals, and upload status signals (high/low electrical level) to the
upper control unit via interfaces.
Interface Definition
The position of sample transport interface on the board is shown in Figure 4-42.
4-122
Hardware System
There is 1 interface between the board and each sensor of the sample transport unit; see
Table 4-108 for their definitions.
Table 4-108 The interface between the sample transport board and each sensor of the
sample transport unit (J1)
Electrical Electrical
Pin Name Description Pin Name Description
level level
Positive end
of the
+5V power light-emitting 5VDC
1 VCC 2 ANODE_TUBE
supply tube of tube 10mA
detection
5VDC photocoupler
50mA Negative end
of the
light-emitting 2.5VDC
3 GND Ground 4 CATHODE_TUBE
tube of tube 10mA
detection
photocoupler
4-123
Hardware System
Start
position
signal of
+5V power 5VDC
5 XL_S_FS the X 6 VCC
supply 50mA
direction
loading
motor
Tube
+5V power
7 VCC 8 HAVE detection
supply 5VDC
signal
50mA
+5V power
9 GND Ground 10 VCC
supply
Stop
position
signal of
5VDC
11 XL_E_FS the X 12 GND Ground
50mA
direction
loading
motor
+5V power +5V power
13 VCC 14 VCC
supply supply
5VDC Sample
50mA volume
15 GND Ground 16 SAMPLEQ
detection
signal
Start
position
signal of
+5V power
17 YRET_FS the Y 18 VCC
supply
direction
5VDC
feeding
50mA
motor
+5V power
19 VCC 20 GND Ground
supply 5VDC
50mA +5V power
21 GND Ground 22 VCC
supply
Signal of
the right
position of Unloader
23 YRIGHT_FS 24 XUL_S
Y direction reset signal
feeding
motor
+5V power 5VDC +5V power 5VDC
25 VCC 26 VCC
supply 50mA supply 50mA
4-124
Hardware System
There are 2 interfaces between the sample transport board and the X direction and Y
direction step motors of the sample transport unit. See Table 4-109 - Table 4-110.
Table 4-109 Interfaces between the sample transport board and the X direction and Y
direction step motors of the sample transport unit (J3)
Electrical
Pin Name Description
level
Connecting the BN
1 ASLX-BN phase of the X
direction motor
Connecting the B
2 ASLX-B phase of the X
direction motor
24VDC/1A
Connecting the AN
3 ASLX-AN phase of the X
direction motor
Connecting the A
4 ASLX-A phase of the X
direction motor
Table 4-110 Interfaces between the sample transport board and the X direction and Y
direction step motors of the sample transport unit (J4)
Electrical
Pin Name Description
level
4-125
Hardware System
Connecting the BN
1 ASY-BN phase of the Y
direction motor
Connecting the B
2 ASY-B phase of the Y
direction motor
Connecting the AN
4 ASY-AN phase of the Y
direction motor
Connecting the A
5 ASY-A phase of the Y
direction motor
There are 3 interfaces between the sample transport board and the driver board. See Table
4-111 - Table 4-113.
Table 4-111 Interfaces between the sample transport board and the sample
transporting sensors of the driver board (J2)
4-126
Hardware System
Table 4-112 Interfaces between the sample transport board and the sample
transporting motors of the driver board (J5)
Electrical
Pin Name Description
level
Connecting the BN phase control terminal of the X direction
1 ASLX-BN
motors
Connecting the B phase control terminal of the X direction
2 ASLX-B
motors
4-127
Hardware System
Table 4-113 Interfaces between sample transport board and the scanner of the driver
board (J6)
4 GND Ground /
7 SCANNER_OUT1 Scanner output signal 1 5VDC/10mA
Scanner TRIGGER
9 SCANNER_TRIGGER 5VDC/1mA
signal
Assembly Drawing
The assembly drawing of the sample transport board is shown in Figure 4-43.
4-128
Hardware System
4-129
Hardware System
Tool
102 cross-headed screwdriver
Removal
Install the board per the above-mentioned procedure in reverse order.
Installation
Verification
1. Install the sample transport board in the analyzer by fixing the screws and
connecting cables to the board properly. Start up the analyzer.
2. Select autoloader mode and start counting, check if any errors concerning the
autoloader unit are reported.
4.10.5 Troubleshooting
4-130
Hardware System
The table below introduces the circuit problems concerning sensors in the sample transport
unit and the ways to troubleshoot them.
4-131
Hardware System
Table 4-115 Error analysis and processing of the sample transport board
4-132
Hardware System
The output
current of the
constant
current
source is too
great; the
Replace the
voltage of U5 Low
component
is above 2.6V
or the
resistance
value of R16
is below 265
ohm.
U3 or U2 is
damaged (or
Board When tubes
of dry joint);
error are not
or R29 and
(suppose available, the
Over-detected R30 are Re-joint or replace
3 it is due voltage of Low
tubes erroneous the components.
to circuit TP2 is above
(incorrect
problem 0.75V, while
resistance
only) the voltage
value or dry
of TP5 is of
joint).
high
Pin 5 of J2 is
electrical
short-circuited
level Low Replug the cable
to the power
supply
When tubes The
are not resistance
Re-joint or replace
available, the value of R8 is Low
the component.
voltage of erroneous or
TP2 is less it is of dry joint
than 0.75V The tube
detection Replace the
Medium
photocoupler component.
is erroneous
4-133
Hardware System
The output
current of the
constant
current
source is too
small; the
Replace the
voltage of U5 Low
component
(TP4) is
below 2.4V or
the resistance
value of R16
is above 275
ohm.
U5 is
erroneous, or
The the resistance
reference values of R11,
Replace the
voltage of U5 R13, R14 and Low
component
(TP4) is not R16 are
2.4-2.6V erroneous, or
Q2 is
erroneous
Pin 13 of J2 is
short-circuited
Low Replug the cable
to the power
Reference
supply
voltage of the
Control signal
constant Board
4 output from
current error
the driver See the
source is
The board is troubleshooting
incorrect Low
reference erroneous section of the driver
voltage of U5 (output high board
(TP4) is electrical
almost zero level)
or too small Q1 or Q2 is
erroneous (or
of dry joint),
or R11, R13, Re-joint or replace
Low
R14 and R16 the component.
are erroneous
(or of dry
joint)
4-134
Hardware System
Board
error Pin 8 of J2 R6 and R20
Start position
(suppose outputs are erroneous
circuit error Re-joint or replace
5 it is due abnormal or U1 is Low
the X direction the component.
to circuit electrical erroneous (or
loading motor
problem level of dry joint)
only)
Board
error Pin 6 of J2 R5 and R21
Stop position
(suppose outputs are erroneous
circuit error of Re-joint or replace
6 it is due abnormal or U1 is Low
the X direction the component.
to circuit electrical erroneous (or
loading motor
problem level of dry joint)
only)
Board
error Pin 4 of J2 R4 and R22
Start position
(suppose outputs are erroneous
circuit error Re-joint or replace
7 it is due abnormal or U1 is Low
the Y direction the component.
to circuit electrical erroneous (or
feeding motor
problem level of dry joint)
only)
Board
error Pin 3 of J2 R3 and R23
Right position
(suppose outputs are erroneous
circuit error of Re-joint or replace
8 it is due abnormal or U1 is Low
the Y direction the component.
to circuit electrical erroneous (or
feeding motor
problem level of dry joint)
only)
Board
error Pin 1 of J2 R2 and R24
Left position
(suppose outputs are erroneous
circuit error of Re-joint or replace
9 it is due abnormal or U1 is Low
the Y direction the component.
to circuit electrical erroneous (or
feeding motor
problem level of dry joint)
only)
Board
error Pin 2 of J2 R1 and R25
X direction (suppose outputs are erroneous
Re-joint or replace
10 loading circuit it is due abnormal or U1 is Low
the component.
error to circuit electrical erroneous (or
problem level of dry joint)
only)
4-135
Hardware System
Board
error Pin 12 of J2 R7 and R26
Unloader
(suppose outputs are erroneous
reset Re-joint or replace
11 it is due abnormal or U1 is Low
detection the component.
to circuit electrical erroneous (or
circuit error
problem level of dry joint)
only)
Pin 10 of J2
outputs high
electrical
U3 or U2 is
level while
damaged (or
Board the voltage
of dry joint);
error of TP1 is
or R27 and
Unloader tray (suppose less than
R28 are Re-joint or replace
12 detection it is due 0.2V; or pin Low
erroneous the component.
circuit error to circuit 10 of J2
(incorrect
problem outputs low
resistance
only) electrical
value or dry
level while
joint).
the voltage
of TP1 is
above 0.3V
Pin 7 of J2
outputs high
electrical U4 or U2 is
level while damaged (or
Board
the voltage of dry joint);
error
Sample of TP1 is or R31 and
(suppose
volume less than R32 are Re-joint or replace
13 it is due Low
detection 0.2V; or pin 7 erroneous the component.
to circuit
circuit error of J2 outputs (incorrect
problem
low electrical resistance
only)
level while value or dry
the voltage joint).
of TP1 is
above 0.3V
4-136
Hardware System
Function
DIFF and BASO DC stirring motor on/off and speed control;
Sample signal detection during piercing and aspiration (pre-blood);
Interface connecting and compatibility.
See Figure 4-44 for the functional block diagram of the sample detecting board.
4-137
Hardware System
4.11.3 Description
Motor on/off control:
The sample detecting board monitors the motor on/off signal from the driver board, when start
signal is detected, the motor driving circuit turns on, otherwise it is off.
Motor speed control:
The sample detecting board divides the signals sent from the motor speed detecting
photocoupler into two paths. One is sent to the driver board for the monitoring of motor speed;
the other is sent to the MCU on the sample detecting board for closed loop control of motor
speed: the MCU can calculate motor speed using its internal timer, judge motor speed range,
and adjust the control signal duty ratio of the motor driving circuit, so that the duty ratio of
motor driving voltage can be adjusted and the rotation speed of motors can be stabilized.
Detecting and converting pre-blood signals
The sample detecting board finishes the I-V converting, voltage following and level converting
of the signals sent from the pre-blood sample detecting sensors, and finally sends the signals
to the driver board.
4-138
Hardware System
J8 J7 J6 J1
J4
J5
J3
J9
J2
4-139
Hardware System
J2 is connected to sample detecting sensors. It provides power supply and signal input for the
sensor. See Table 4-117 for the distribution and definition of its pins.
J3 is connected to the J21 of the driver board. It provides voltage for the sample detecting
board and outputs the detecting signal. The distribution and definition of its pins are the same
as those of J21 on the driver board, which is illustrated in 4.3.2 of this manual.
J4 is connected to sensors of the syringe unit and stirring motors. It provides power supply and
signal input for the syringe position sensor and the stirring motor speed sensor. See Table
4-118 for the distribution and definition of its pins.
4-140
Hardware System
J5 is the on/ff control signal input end of the stirring motor, it is connected to J31 on
the driver board. See Table 4-119 for the distribution and definition of its pins.
Signal
Pin No. Name Description
Type
DCM2_IN DIFF stirring motor on/off control
1 TTL
signals
2 VCC \ +5V
DCM1_IN BASO stirring motor on/off
3 TTL
control signals
4 VCC \ +5V
4-141
Hardware System
J7 is the driving interface of 12V DC motor; it is connected with DIFF and BASO stirring motors.
See Table 4-120 for the distribution and definition of its pins.
Signal
Pin No. Name Description
Type
DCM2_ON DIFF stirring motor driving
1 \
signals
2 12V0 \ +12V
DCM1_ON BASO stirring motor driving
3 \
signals
4 12V0 \ +12V
J8 is the 12V power input end of the sample detecting board. It provides power supply to the
12V motor and is connected to J4 on the power board. See Table 4-121 for the distribution and
definition of its pins.
Signal
Pin No. Name Description
Type
Power
1 \ \
ground
2 12V0 \ +12V
J9 is the interface for program downloading and debugging of the MCU on the sample
detecting board. It is not connected with any other boards. See Table 4-122 for the distribution
and definition of its pins.
Description Signal
Pin No. Name
Type
Receiving end of serial
1 PC_RX ports of the upper control TTL
unit
Sending end of serial
2 PC_TX ports of the upper control TTL
unit
3 DGND Digital ground /
4 PSEN Program enabling end TTL
4-142
Hardware System
(not in use)
Reset end (low level
5 RESET TTL
reset)
Assembly Drawing
See Figure 4-46 for the assembly drawing of the sample detecting board.
Indicator Description
D2 3.3V power indicator
D8 MCU working status indicator, flashes every 0.5s when the mid control
unit runs normally
4-143
Hardware System
Tool
101 cross-headed screwdriver
107 cross-headed screwdriver
Removal
1. Open the top cover (refer to Section 5.2.4 for details);
2. Loosen and remove the two small panhead screws (M4X8) which fix the main board
and the signal board assembly with 107 cross-headed screwdriver;
3. Turn up the main board and the signal board assemblies and support them with the
PCB assembly stop bar;
4. Pull out the three electric plugs on the sample detecting board;
5. Loosen the 4 cross-recessed panhead screws (M2X6) (They are used to fix the
sample detecting board) with 101 cross-headed screwdriver and remove the sample
detecting board (Figure 4-47);
4-144
Hardware System
Installation
Installation is the opposite operation of removal.
Note:
Verification
1. Inspect whether all components are installed and fixed well;
4. In auto-sampling mode, conduct one normal sample test and one blank background
test successively. If "Insufficient aspiration" are not prompted for the normal sample,
but "Abnormal sample" or "Insufficient aspiration" can be prompted in background test,
then the replacement is successful.
5. If abnormal speed of stirring motors were reported before the replacement and the
error disappears after the replacement, that means the replacement is successful.
4-145
Hardware System
4.11.7 Troubleshooting
Table 4-125 Error analysis and processing of the sample detecting board
Serial
Description Error analysis and processing
No.
1 Possible causes: There is something wrong
with the input voltage +5V; Pre-blood sample
detecting sensor cannot work well; The optical
path of the pre-blood sample sensor is abnormal.
2 Solutions: Measure the voltage of TP7 with
multimeter. If the voltage is within the range of
+5V0.25V, then it is not the problem of the power
supply. If the input voltage exceeds the above range,
then inspect J21.13 and connection cables of the
driver board and solve the error of the power supply.
When there is no blood in If the +5V power supply works well, inspect whether
1 pre-blood detecting tube, the optical path of the pre-blood sample detecting
VTP1<1.5V sensor is covered by dirt. If yes, eliminate the dirt
and restore the optical path. Otherwise there is no
problem with the optical path. If the power supply
and optical path both work well, then let air into the
pre-blood detecting tube, and adjust the variable
resistance VR1. If we can make VTP11.5V, then
after VTP11.5V, fix the variable resistance VR1, and
the error of the pre-blood sensor can be eliminated.
If after adjustment of VR1, it cannot be 1.5V, then the
pre-blood sensor is damaged and needs to be
replaced.
1 Possible causes: R6 is open-circuited; D1 is
When there is no blood in
open-circuited; Q1 is damaged
the pre-blood detecting
2 2 Solutions: Measure R6 or D1 with multimeter. If
tube and VTP1 1.5V,
there are not open-circuited, then Q1 is damaged
then VFRONT>0.8V
and needs to be replaced.
1 Possible causes: The optical path of the sensor
is abnormal; The pre-blood sensor is damaged.
When the pre-blood 2 Solutions: Inspect the installation of the pre-blood
3 detecting tube is full of sensor. If it is installed correctly, then the optical path
blood, VTP2 1.5V is normal. Otherwise install it correctly. If the optical
path is normal, then the pre-blood sensor is
damaged and needs to be replaced.
4-146
Hardware System
First check whether the abnormities in BASO and DIFF stirring motors are reported at the
same time. If yes, check whether J3, J4, J5, J7, J8 and J21 are properly connected with cables.
If the cables are well connected, check whether MCU indicator D8 on the sample detecting
board flashes. If it flashes normally, that means there is no problem with the power supply. If
D8 is always on, then the MCU U2 is damaged, the sample detecting board needs to be
replaced. If D2 is off, check the voltage of TP5 with a multimeter to see if it is within the range
of+5V0.25V. If it not within the range, that means something is wrong with the input power
supply, please check its connection cable with the driver board; if the voltage of TP5 is normal,
then the power conversion chip U6 is damaged, the sample detecting board needs to be
replaced.
If speed abnormity is only reported for BASO (or DIFF) stirring motors, please view the speed
of BASO (or DIFF) motors on the self-test screen, if its speed is less than 1200 turns per
second, that means the motors are damaged and need to be replaced. If the speed of BASO
(or DIFF) motors is zero, but they can rotate normally, that means something is wrong with the
photocoupler in BASO (or DIFF) channel. Please check if the photocoupler is covered by dirt, if
yes, clear the dirt. If the error persists after the above operation, that means the photocoupler
is damaged and needs to be replace. If the speed of the motors is zero, and motors do not
move, the BASO (or DIFF) motor driving circuit is damaged, the sample detecting board needs
to be replaced.
4-147
Hardware System
Function
Detecting pressure of the vacuum chamber, the pressure chamber and the sheath fluid.
See Figure 4-48 Block diagram of the pressure detecting board for the functional block
diagram of the pressure detecting board.
250KPa
160KPa
70KPa J1 J19
-40KPa
Pressure
-85KPa
detecting Driver
board board
4.12.3 Description
The pressure detecting board converts pressure into voltage signals via 5 channels of
pressure detecting chips, and then outputs the voltage signals to the driver board, which
collects and calculates the voltage signals.
4-148
Hardware System
J1 is the only external interface on the pressure detecting board. It has 8 pins. See below for
definitions of the pins:
Signal
Pin No. Name Description
Type
PSC_1 Signal output of 250Kpa channel Analog
1
pressure detection signal
PSC_2 Signal output of 160Kpa channel Analog
2
pressure detection signal
PSC_3 Signal output of 70Kpa channel Analog
3
pressure detection signal
PSC_4 Signal output of -40Kpa channel Analog
4
pressure detection signal
PSC_5 Signal output of -85KPa channel Analog
5
pressure detection signal
6 PSC_6 Reserved channel (not in use) /
7 AVCC 5V power input /
8 AGND Analog ground /
Assembly Drawing
See Figure 4-50 for the assembly drawing of the pressure detecting board.
4-149
Hardware System
4-150
Hardware System
Tool
101 cross-headed screwdriver
107 cross-headed screwdriver
Removal
1. Open the top cover (refer to Section 5.2.4 for details);
2. Loosen and remove the two small panhead screws (M4X8) which fix the main board
and the signal board assembly with 107 cross-headed screwdriver;
3. Turn up the main board and the signal board assemblies and support them with the
PCB assembly stop bar;
5. Loosen and remove the two small panhead screws (M4X8) which fix the pressure
detecting board assembly with 107 cross-headed screwdriver;
6. Unplug the 5 pneumatic tubes connected to the pressure detecting board and remove
the board assembly;
7. Loosen the 4 cross-recessed panhead screws (M2X6) (They are used to fix the
pressure detecting board) with 101 cross-headed screwdriver and remove the board.
Installation
Installation is the opposite operation of removal.
Note:
Verification
1. Inspect whether all components are installed and fixed well;
3. Inspect whether the pneumatic tubes are correctly connected with the board and
whether there is air leakage.
4. Check whether the pressures of the 5 channels displayed on the self-test screen is
within the ranges listed in below table:
Pressure
Pressure range
channel
250kPa 225kPa-275kPa
160kPa 155kPa-165kPa
70kPa 65kPa-75Kpa
-40kPa -43kPa-37kPa
-85kPa -82kPa-88kPa
4-151
Hardware System
5. If the pressures of the 5 channels are within above ranges, that means the pressure
detecting board is replaced successfully, otherwise troubleshooting it required.
4.12.7 Troubleshooting
Troubleshooting abnormal pneumatic pressure
1. If all vacuum and pressure of the system are zero, check the connection between the
pressure detecting board and the driver board/pneumatic tubing, and whether the
pneumatic unit starts normally;
2. If all vacuum and pressure of the system are abnormal and with the too high or too
low pressure value at the same time, check whether the air compressor works
normally. See Chapter 5 (pneumatic system troubleshooting) for a reference of the
method;
3. If only one pressure value is abnormal, check the related regulator and airway for
leakage.
4. If the airway system is normal, the error is due to the pressure sensor failure on the
driver board. Replace the driver board.
See the following table for the output voltage and pressure of the pressure sensors:
Pressure Test Relation between sensor output Mid-point
Description
channel Point voltage and pressure value voltage
P represents
250kPa TP1 P = Vout / 0.0045 44.4 25 1.325
pressure, and
160kPa TP2 3.8 its unit is kPa;
P = Vout / 0.0225 8.89 5
70kPa TP3 1.775 Vout represents
-40kPa TP4 2 the voltage of
P = Vout / 0.045 + 4.44 2.5 the test point,
-85kPa TP5 4.025
and its unit is V.
4-152
Hardware System
3. Select one pressure channel from the "Pressure" tab, e.g. PS (250), the PS1 pressure
calibrate dialog box will pop up;
4. Fill the A pressure of PS1 channel measured by barometer into "Apparatus", and then
select "Calc.", calibration factor K will be displayed in "New fac.". Click "Save K" without
record the calibration factor, the PS1 pressure calibrate screen will close automatically. PS1
pressure calibration is done.
5. Repeat Step 3 and 4 to calibrate other 4 channels. After calibrate all channels, the
calibration job required after replacing the pressure detecting board is completed.
4-153
Hardware System
1. Do not adjust pressure valve randomly before error of the pressure detecting board is
confirmed;
2. After replace the board, enter software screens "Service""Status""Temp. &Pres.". See
below figure:
3. Check whether the deviations between the values of the 5 channels and the standard value
are greater than 10%. If yes, that means something is wrong with the pressure system, the
pneumatic unit and pressure valves need to be checked and calibration cannot be performed.
If no, go to Step 4 - pressure calibration.
4. Select "Self-test""T&P Cal.". See Figure 4-51.
5. Select one pressure channel from the "Pressure" tab, e.g. PS1(250), the PS1 pressure
calibrate dialog box will pop up; see Figure 4-52. Enter 250 into "Apparatus", click "Calc.",
and then click "Save K" to finish calibration of the channel.
6. Repeat Step 5 to calibrate other 4 channels. Enter 160, 70, -40 and -85 in to the
"Apparatus" of PS2, PS3, PS4 and PS5 on the calibrate dialog box, and then click "Calc."
and "Save K".
7. After calibrate the pressures of the 5 channels, the calibration job required after replacing
the pressure detecting board is completed.
4-154
Hardware System
interface to SATA interface, meeting the product requirement of using hard disk of SATA
interface.
Interface Definition
J1 is the SATA interface on the board; its definition is as follow:
4-155
Hardware System
Figure 4-57 Assembly drawing of the SATA and IDE signal conversion board
4-156
Hardware System
Tool
107 cross-headed screwdriver
Removal
1. Open the top cover (refer to Section 5.2.4 for details);
2. Loosen and remove the two small panhead screws (M4X8) which fix the main board
and the signal board assembly with 107 cross-headed screwdriver;
3. Turn up the main board and the signal board assemblies and support them with the
PCB assembly stop bar;
4. Unplug hardware data wire and power wire of the hard disk, remove the 4 M4X8
screws from the hard disk assembly; see Figure 4-58;
5. Take off the two 3X8 screws fixing the conversion board to the hard disk.
4-157
Hardware System
z Please make sure power is off and the power cord is disconnected when
mounting and removing boards.
Installation
Installation is the opposite operation of removal.
Verification
Connect the power cord and switch on power switch, if the analyzer starts and runs normally,
that means the hard disk is visited correctly.
Troubleshooting
Error description:
The hard disk cannot be visited during starting up, the analyzer fails to start.
Possible causes:
Invalid connection. Connection of the power cord, IDE socket or the SATA interface to the hard
disk is loose; the hard disk is broken; or the SATA and IDE signal conversion board or main
board is broken.
Solutions:
1. Turn off power supply, replug the above connectors and fix them properly. Turn on
power supply to check if the analyzer restores normal status;
2. Turn off power supply, replace the SATA and IDE signal conversion board or main
board, and fix them properly. Turn on power supply to check if the analyzer restores
normal status;
3. Turn off power supply, replace the hard disk and fix it properly. Turn on power supply
to check if the analyzer restores normal status.
4-158
Servicing
5 Servicing
5.1 General
You can service the BC-5800 as per Section 5.2 5.16. For the repairing with verification, you
must follow the procedures strictly to verify the maintenance.
5-1
Servicing
To disassemble the pneumatic/electric/fluidic parts, etc. inside the analyzer, follow the
procedures to remove the left door.
Tools
Removal
1. Power off the analyzer and unplug the power cord from the wall outlet.
2. Use the 107 screwdriver to unscrew the 3 M4X8 screws as per Figure 5-1.
3. Pull the left door backwards to make it unlocked from the front plate and bottom plate,
and then remove the left door as per Figure 5-1.
Installation
5-2
Servicing
Install the left door according to the steps stated above in reverse order.
Follow the procedures to remove the right door to disassemble the fluidic parts inside the
analyzer.
Tools
Removal
1. Power off the analyzer and unplug the power cord from the wall outlet.
2. Use the 107 screwdriver to unscrew the 3 M4X8 screws as per Figure 5-2.
3. Pull the right door backwards to make it unlocked from the front plate and bottom
plate, then remove the right door as Figure 5-2.
Installation
Install the right door according to the steps stated above in reverse order.
5-3
Servicing
Follow the procedures to open the upper cover to disassemble the components on the front
plate.
Removal
Open the upper cover and support it with the stop bar as per
Figure 5-3.
Installation
Lift up the upper cover, release the stop bar and then put down the upper cover.
5-4
Servicing
Follow the procedures to remove the top cover to disassemble the optical components, boards,
cables and connectors.
Tools
Removal
1. Power off the analyzer and unplug the power cord from the wall outlet.
3. Use the 107 screwdriver to unscrew the 5 M4X8 screws, 3 on the back plate and 2 on
the front plate (see Figure 5-4).
4. Pull the top cover backwards for 10-30mm, then remove it up from the main unit as
Figure 5-4.
Installation
Install the top cover according to the steps stated above in reverse order.
5-5
Servicing
Follow the procedures to remove the lower cover to disassemble the syringe assembly, mixing
& piercing assembly, START switch assembly and reagent preheating assembly.
Tools
Removal
Due to different configurations, you can follow these three procedures to remove the lower
cover.
To remove the lower cover from the analyzer with sample transport unit
1. Power off the analyzer and unplug the power cord from the wall outlet.
5. Use the 107 screwdriver to loosen the 2 M3X8 screws fixing the protective cover, then
remove the protective cover as Figure 5-5.
5-6
Servicing
z When transporting the analyzer, combine the sample transport unit with the
protective cover by fixing 2 M3X8 screws shown in the two circles in Figure
5-5. However, you need not install the two screws when installing the
analyzer.
6. Use the 107 screwdriver to unscrew the 2 M4X8 screws fixing the sample transport
unit, then move the transport unit outwards and pull out the connecting cables as
show in Figure 5-6.
7. Unplug J2 and J5 connectors from the adapter as shown in Figure 5-7, and then
remove the sample transport unit.
5-7
Servicing
8. Use the 107 screwdriver to unscrew the 8 screws fixing the lower cover, 4 M4X8
screws in the middle and 2 M4X12 screws on each side, as shown in Figure 5-8.
5-8
Servicing
To remove the lower cover from the analyzer with only open vial sampler
1. Power off the analyzer and unplug the power cord from the wall outlet.
5. Use the 107 screwdriver to unscrew the 4 M3X8 screws fixing the enclosure of the
closed tube sampler, then remove the enclosure as Figure 5-9.
5-9
Servicing
Figure 5-9 Removing the lower cover (with open vial sampler)-1
6. Use the 107 screwdriver to unscrew the 8 screws fixing the lower cover, 4 M4X8
screws in the middle and 2 M4X12 screws on each side, as shown in Figure 5-10.
5-10
Servicing
Figure 5-10 Removing the lower cover (with open vial sampler)-2
Installation
Install the lower cover according to the steps stated above in reverse order.
5-11
Servicing
5.3.2 Tools
107 cross-headed screwdriver
100mm monkey wrench
5.3.3 Removal
Follow these two procedures to disassemble the power supply assembly.
1. Power off the analyzer and unplug the power cord from the wall outlet.
3. Use the 107 screwdriver to unscrew the M4X8 screw fixing the waterproof cover, and
then remove the waterproof cover as Figure 5-11.
5-12
Servicing
4 3 2 1
4. Unplug J2, J3, J4 and J5 connectors from the power board as Figure 5-12.
5. Use the 107 screwdriver to loosen the 4 M4X8 screws fixing the power supply
assembly, and then remove the power supply assembly as Figure 5-11
1. Use the 107 screwdriver to unscrew the 4 M3X8 screws fixing the power supply
housing as shown in Figure 5-12.
2. Unplug J6 from the power board and remove the power supply housing as Figure
5-12.
3. Use the 107 screwdriver and 100mm monkey wrench to loosen the M4X20 screws
and M4 nuts fixing the fans, then remove the fans as Figure 5-12.
4. Unplug connector from J1 of the power board. Use the 107 screwdriver to unscrew
the 9 M3X8 screws fixing the power board, and then remove the power board as
5-13
Servicing
Figure 5-12.
5. Disconnect the electric connectors from the power switch terminals. Use the 107
screwdriver to unscrew the M4X8 screw fixing the earth wire as Figure 5-12.
6. Use the 107 screwdriver to unscrew the 2 M3X6 screws fixing the 3516 socket, then
remove the socket as Figure 5-12.
5.3.4 Installation
Install the power supply assembly according to the steps stated above in reverse order.
Precautions:
5-14
Servicing
Brown indicates the live wire and blue for the neutral wire.
Connect the upper connector of the - terminal to the live wire of the power board
and the lower connector to the neutral wire.
Connect the upper connector of the O terminal to the live wire of the power socket
and the lower connecter to the neutral wire.
Make sure to connect the two upper connectors to the brown wires and the two lower
connectors to the blue wires.
2. Direct the earth terminal of the 3516 power socket downwards as Figure 5-13.
3. Install the fans with the text side facing the outside and in the same direction.
5.3.5 Verification
1. Check whether all components are installed and fixed in place.
2. Check whether the power switch connections, the 3516 power socket and fan
direction meet the requirements.
5-15
Servicing
5.4.2 Tools
101 cross-headed screwdriver
107 cross-headed screwdriver
5.4.3 Removal
Follow these three procedures to disassemble the touchscreen assembly.
1. Power off the analyzer and unplug the power cord from the wall outlet.
3. Unplug the connector from J1 of the indicator board and the connectors from J5 of the
touch screen control board as Figure 5-14.
4. Use the 107 screwdriver to unscrew the M4X8 screw fixing the screen ground wire, as
Figure 5-14.
7. Use the 107 screwdriver to unscrew the two M3X6 screws fixing the main board
shielding cover to remove the main board shielding cover as Figure 5-14.
8. Unplug the connecter connecting the J2 and J16 of the main board, remove the
grounding screw and then remove the signal cable of the TFT displayer and the
power cord of the TFT screen converter as Figure 5-14.
9. Use the 107 screwdriver to unscrew the six PT3X10 screws fixing the touchscreen
assembly to remove the touchscreen assembly as Figure 5-14.
5-16
Servicing
1. Use the 107 screwdriver to loosen the 6 PT3X10 tapping screws fixing the upper
pressure plate and lower pressure plate. Remove the pressure plates with sponge
pads, as shown in Figure 5-15.
5-17
Servicing
1. Use the 107 screwdriver to remove the 2 M3X6 cross-recessed panhead screws
fixing the touchscreen control board, and then remove the touchscreen control board,
as shown in Figure 5-16;
2. Use the 107 screwdriver to remove the 2 M3X6 cross-recessed panhead screws
fixing the converter shielding box, and then remove the shielding box, as shown in
Figure 5-16;
3. Remove the 2 connectors connecting the converter. Use the 101 screwdriver to
remove the 2 M2X4 cross-recessed panhead screws fixing the converter, and then
remove the converter, as shown in Figure 5-16;
4. Use the 101 screwdriver to remove the 4 M2X8 cross-recessed panhead screws
fixing the LCD screen and the 2 plain washer, and then remove the LCD screen, as
shown in Figure 5-16;
5-18
Servicing
5.4.4 Installation
Follow these three procedures to install the touchscreen assembly
Assemble the touchscreen according to the steps stated above in the reverse order.
5-19
Servicing
2. Fix the upper and lower pressure plates with sponge pads on the cover by securing
the 6 PT3X10 tapping screws using the 107 screwdriver, as shown in Figure 5-17;
3. Affix two buffer pads to the left and right of the touchscreen, and cut off the parts
extending over the edges of the touchscreen.
1. Put the FPC cable of the touchscreen through the hole in the screen mounting plate,
and then secure the 6 PT3X10 tapping screws using the 107 screwdriver, as shown in
Figure 5-14;
3. Fix the shielding cover of the control board to the screen mounting plate by securing 3
M3X6 cross-recessed panhead screws using the 107 screwdriver.
Do not press the touchscreen against the locating frame during installation, or the
touchscreen may be cracked. Check the position of the touchscreen after securing
the upper and lower pressure plate, and make sure it is right inside the locating
frame.
Crop the buffer pads to make sure they do not extend over the edges of the
touchscreen.
5-20
Servicing
The insulating strip of the backlight board can be removed for reuse.
5.4.5 Verification
1. Check whether all components are installed and fixed in place.
3. Start up the analyzer and check the touching and displaying of the screen.
5-21
Servicing
Tools
101 cross-headed screwdriver
107 cross-headed screwdriver
100mm monkey wrench
5-22
Servicing
Removal
Follow these five procedures to disassemble the gas valve assembly.
1. Power off the analyzer and unplug the power cord from the wall outlet.
5. Use the 107 screwdriver to loosen the 4 M4X8 screws fixing the gas valve assembly,
and then remove the gas valve assembly. The screws are indicated in black circles in
Figure 5-19.
1. Power off the analyzer and unplug the power cord from the wall outlet.
3. Use the 101 screwdriver to unscrew the 2 M3X12 screws fixing the valve.
4. Disconnect all the tubes connected to the valve and remove the valve as shown in
Figure 5-20.
5-23
Servicing
You can follow the procedures to remove the five-way gas valve GV92 from the gas valve
assembly as shown in Figure 5-18.
1. Power off the analyzer and unplug the power cord from the wall outlet.
5. Use the 107 screwdriver to unscrew the 2 M3X6 screws fixing the GV92 valve, and
then remove the valve as shown in Figure 5-20.
6. Use the 100mm wrench to unscrew the 3 LC-0425-M3 connectors on the GV92 valve
as Figure 5-20.
There are 2 manifolds on the gas valve assembly as shown in Figure 5-18. All valves on the
manifolds can be installed and removed in the same way.
5-24
Servicing
1. Power off the analyzer and unplug the power cord from the wall outlet.
4. Use the 101 screwdriver to unscrew the 2 M2X16 screws fixing the valve, and then
remove the valve as per Figure 5-20.
There are 2 manifolds on the gas valve assembly as shown in Figure 5-18. Each manifold can
be installed and removed in the same way. Removal of the manifold 2 is described below.
1. Power off the analyzer and unplug the power cord from the wall outlet.
5. Use the 107 screwdriver to unscrew the 2 M3X20 screws fixing the manifold 2, then
remove the manifold as per Figure 5-20.
6. Use the 101 screwdriver and 100mm wrench to disassemble the manifold 2 as per
Figure 5-21.
5-25
Servicing
Installation
Install the gas valve assembly according to the steps stated above in reverse order.
Precautions:
Verification
1. Check whether all components are installed and fixed in place.
5-26
Servicing
Tools
104S-#1(38) cross-headed screwdriver
150mm monkey wrench
Removal
Follow these two procedures to disassemble the regulating valve assembly.
1. Power off the analyzer and unplug the power cord from the wall outlet.
4. Use the 104S-#1(38) screwdriver to unscrew the 3 M4X12 screws fixing the
regulating valve assembly, and then remove the assembly as per Figure 5-22.
5-27
Servicing
1. Loosen the cap and remove the vacuum regulating valve as per Figure 5-23.
2. Loosen the stem and stem locking nut of the precision regulating valve as per Figure
5-23.
3. Use the 150mm wrench to unscrew the valve locking nut, then remove the precision
regulating valve as per Figure 5-23.
4. Use the 150mm wrench to unscrew the L-shape and Y-shape connectors from the
vacuum regulating valve and precision regulating valve as per Figure 5-23.
5-28
Servicing
3
4
Installation
Install the regulating valve assembly according to the steps stated above in reverse order.
Precautions:
1. Power on the analyzer, adjust the three regulating valves as required, then lock the
regulating valves by:
5-29
Servicing
3. Fixing the stem locking nut of the precision regulating valve. Secure the regulating
valve with the wrench shown in Figure 5-24.
Verification
1. Check whether all components are installed and fixed in place.
Tools
107 cross-headed screwdriver
Removal
Follow these two procedures to disassemble the pinch valve assembly.
1. Power off the analyzer and unplug the power cord from the wall outlet.
5. Use the 107 screwdriver to loosen the 2 M4X8 screws fixing the pinch valve assembly,
and then remove the pinch valve assembly as per Figure 5-25.
5-30
Servicing
1 2 3
Use the 107 screwdriver to disassemble the pinch valve assembly as per Figure 5-26.
5-31
Servicing
Installation
Install the pinch valve assembly according to the steps stated above in reverse order.
Precautions:
Connect the tubing as per Appendix E.
Verification
1. Check whether all components are installed and fixed in place.
Removal
Follow these two procedures to disassemble the valve/pump assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
4. Use the 107 screwdriver to unscrew the 6 M4X8 screws fixing the valve/pump
assembly, then move out the valve/pump assembly. The screws are indicated in black
circles in Figure 5-27.
5-32
Servicing
There are 17 two-way and 9 three-way Mindray valves on the valve/pump assembly. Each of
the valves can be installed and removed in the same way. Removal of the three-way valve
SV24 is described below.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
4. Use the 107 screwdriver to unscrew the 2 M3X12 screws fixing the SV24 valve, and
then move out the valve as per Figure 5-28.
5-33
Servicing
z Make sure the cables of the solenoid valves do not fall behind the holes in
the valve bracket.
There are one 0.32mL, three 1mL, three 2mL and one 3mL metering pumps on the valve/pump
5-34
Servicing
assembly. Each of the pumps can be installed and removed in the same way. Removal of the
3ml pump is described below.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
3. Unplug the fluid line and pneumatic line from the 3mL metering pump.
4. Use the 107 screwdriver to unscrew the 2 M3X6 screws fixing the 3mL metering
pump, then remove the pump together with the mounting plate as per Figure 5-28.
5. Use a 2.5mm hexagon wrench to unscrew the 2 M3X12 screws fixing the 3mL
metering pump, then remove the metering pump as per Figure 5-29.
Installation
Install the valve/pump assembly according to the steps stated above in reverse order.
Precautions:
5-35
Servicing
Verification
1. Check whether all components are installed and fixed in place.
2. Check whether the cables of the solenoid valves are connected correctly.
Tools
102 flat-headed screwdriver
107 cross-headed screwdriver
2mm hexagon wrench
2.5mm hexagon wrench
150mm monkey wrench
Removal
Follow these two procedures to disassemble the 100l syringe assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
3. Unplug the optical sensor and motor connectors from the 100l syringe assembly.
4. Unplug the two fluidic lines from the 100l syringe assembly. (Note: Theres only one
fluidic line connected to the 2.5mL syringe assembly)
5. Use the 107 screwdriver to unscrew the 4 M3X10 screws fixing the 100l syringe
assembly, and then remove the 100l syringe assembly and four rubber rings as per
Figure 5-30.
5-36
Servicing
1. Use the 2mm wrench to unscrew the M4X8 screw fixing the motor rod, and then
remove the motor rod as per Figure 5-31.
z Make sure to insert the retaining screw into the hole on the motor rod, and
apply some lock glue to the threads.
2. Use the 102 screwdriver to unscrew the M4X8 screw fixing the 100l syringe unit as
per Figure 5-31.
5-37
Servicing
3. Use the 2.5mm wrench to unscrew the 2 M3X16 screws fixing the 100l syringe unit,
and then remove the fixing block and the 100l syringe unit as per Figure 5-31.
4. Use the 150mm wrench to disassemble the 100l syringe as per Figure 5-32.
z Apply some sealing glue to the screw threads. Dont proceed to the next
step until the glue is dry.
5-38
Servicing
5. Use the 2mm wrench to unscrew the M4X8 screw fixing the guiding rod, then remove
the guiding rod and the slider as per Figure 5-33.
z Make sure to attach the retaining screw to the surface of the guide rod, and
apply some lock glue to the threads.
6. Use the 107 screwdriver to unscrew the M4X10 screw fixing the optical sensor, and
then remove the sensor as per Figure 5-33.
7. Use the 2.5mm wrench to unscrew the 4 M3X8 screws fixing the linear step motor,
and then remove the motor as per Figure 5-33.
5-39
Servicing
z Install the motor and the optical sensor with their connecting lines to the
same direction.
8. Use the 2.5mm wrench to unscrew the 4 M3X10 screws fixing the guiding plate, then
remove the guiding plate as per Figure 5-33.
9. Use the 107 screwdriver to disassemble the slider unit as per Figure 5-34.
5-40
Servicing
Installation
Install the syringe assembly according to the steps stated above in reverse order.
Precautions:
1. Connect the step motors and optical sensors to the cables indicated below:
Verification
1. Check whether all components are installed and fixed in place.
5-41
Servicing
Tools
107 cross-headed screwdriver
150mm monkey wrench
Tweezers
Removal
Follow these two procedures to disassemble the cleanser cistern assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
4. Unplug the pneumatic line and 2 fluidic lines connected to the cleanser cistern
assembly.
5. Use the 107 screwdriver to loosen the 2 M4X8 screws fixing the cleanser cistern
assembly, and then remove the assembly as per Figure 5-35.
5-42
Servicing
1. Use the 107 screwdriver to unscrew the 4 M3X12 screws fixing the waste cistern cap,
and then remove the cap and the liquid-level sensor as per Figure 5-36.
2. Use the tweezers to unplug the two connectors on the liquid-level sensor, then use
the 150mm wrench to unscrew the liquid-level sensor as per Figure 5-36.
5-43
Servicing
1----WC2 2----Bracket 2
3----Liquid-level sensor BGRF-F 4----Charging tube
5----Waste cistern cap 6----Cross-recessed panhead screw
M3X12 (with washer)
7----Vacuum cupule 8----O ring 48.7x1.8
9----O ring 9.5x1.8 10----Sealing surface
Installation
Install the cistern assembly according to the steps stated above in reverse order.
Precautions:
5-44
Servicing
Verification
1. Check whether all components are installed and fixed in place.
2. Check whether the liquid-level sensors electric connectors are connected correctly.
4. Power on the analyzer and verify your replacing (including the airtightness test)
z If there is a back flow from the cisterns and the pneumatic unit is down,
there may be reagent left in the tubings between the vacuum sensors and
the vacuum side of the pump, inside the isolation chamber, vacuum relief
valve and pump, In this case, the service personnel shall manually drain the
tubings and the isolation chamber, remove and clear the vacuum relief
valve, check if the pump can operate properly, disassemble and clean the
pump to remove the reagent.
Tools
107 cross-headed screwdriver
150mm monkey wrench
Tweezers
Removal
Follow these two procedures to disassemble the WC1 assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
4. Unplug the pneumatic line and four fluidic lines connected to the WC1 assembly.
5. Use the 107 screwdriver to loosen the 2 M4X8 screws fixing the WC1 assembly, and
then remove the WC1 assembly as per Figure 5-37.
5-45
Servicing
1. Unplug the pneumatic line connecting the waste cistern cap to the anti-back-flow
cistern cap.
2. Use the 107 screwdriver to unscrew the 4 M3X12 screws fixing the waste cistern cap,
and then remove the cap and the liquid-level sensor as per Figure 5-38.
5-46
Servicing
3. Use the tweezers to unplug the two connectors on the liquid-level sensor, then use
the 150mm wrench to unscrew the extension bar and the liquid-level sensor as per
Figure 5-38.
5. Take out manually the big floater fallen into the anti-back-flow cistern.
6. Use the 107 screwdriver to unscrew the 4 M3X12 screws fixing the anti-back-flow
cistern cap, and then remove the anti-back-flow cistern cap as per Figure 5-38.
8. Use the 107 screwdriver to unscrew the 2 M3X8 screws fixing the 8mm pinch valve,
then remove the pinch valve as per Figure 5-38.
1----WC2 2----Bracket 1
3----Liquid-level sensor RF-OV1 4----Charging tube
5----O ring 9.5x1.8 6----Waste cistern cap
5-47
Servicing
Installation
Install the WC1 assembly according to the steps stated above in reverse order.
Precautions:
Verification
1. Check whether all components are installed and fixed in place.
4. Power on the analyzer and verify your replacing (including the airtightness test).
Tools
107 cross-headed screwdriver
150mm monkey wrench
Tweezers
Removal
Follow these two procedures to disassemble the WC2 assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
5-48
Servicing
5. Use the 107 screwdriver to loosen the 2 M4X8 screws fixing the WC2 assembly, and
then remove the WC2 assembly as per Figure 5-39.
1. Use the 107 screwdriver to unscrew the 4 M3X12 screws fixing the waste cistern cap,
and then remove the cap and the liquid-level sensor as per Figure 5-40.
5-49
Servicing
2. Use the tweezers to unplug the two connectors on the liquid-level sensor, then use
the 150mm wrench to unscrew the extension bar and the liquid-level sensor as per
Figure 5-40.
Installation
Install the WC2 assembly according to the steps stated above in reverse order.
Precautions:
5-50
Servicing
Verification
1. Check whether all components are installed and fixed in place.
Tools
107 cross-headed screwdriver
Removal
Follow these three procedures to disassemble the metering tube assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
4. Lift up the main board and signal processing board, lower down the stop bar, then
place down the two boards.
9. Use the 107 screwdriver to loosen the 4 M4X8 screws fixing the metering tube
assembly, and then remove the metering tube assembly as per Figure 5-41.
5-51
Servicing
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
6. Use the 107 screwdriver to unscrew the 4 M3X6 screws fixing the volumetric board,
5-52
Servicing
then remove the volumetric board and the metering tube as per Figure 5-42
8. Refer to the following procedures for removal of solenoid valves. Use the 107
screwdriver to unscrew the 2 M3X12 screws fixing the solenoid valve, and then
remove the valve as per Figure 5-42.
5-53
Servicing
There are 5 two-way and 1 three-way Mindray valves on the metering tube assembly. Each of
the valves can be installed and removed in the same way. Removal of the SV36 valve is
described below.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
5. Use the 107 screwdriver to unscrew the 2 M3X6 screws fixing the SV36 valve as per
Figure 5-42.
Installation
Install the metering tube assembly according to the steps stated above in reverse order.
Precautions:
5-54
Servicing
Verification
1. Check whether all components are installed and fixed in place.
5-55
Servicing
Purpose
Follow the procedures to replace the RBC bath assembly.
Tools
107 cross-headed screwdriver
150mm monkey wrench
Removal
Follow these six procedures to disassemble the RBC bath assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
3. Use the 107 screwdriver to unscrew the 4 M4X8 screws fixing the outer shielding box,
then remove the box (Figure 5-44).
4. Unscrew the retaining screw and remove the door of the outer shielding box (Figure
5-44).
4
3
2
1
5-56
Servicing
Use the 107 screwdriver to unscrew the 4 M3X5 screws fixing the inner shielding box, then
remove the box (Figure 5-45).
3. Use the 107 screwdriver to unscrew the M4X8 screw fixing baffle plate 1 (Figure
5-46).
5. Use the 107 screwdriver to unscrew the 2 M4X8 screws fixing the main board/signal
processing board assembly (Figure 5-46).
5-57
Servicing
6. Lift up the main board/signal processing board assembly, then support it with the stop
bar (Figure 5-47).
5-58
Servicing
1. Lift up the main board/signal processing board assembly, and release the stop bar to
put down the board assembly.
2. Use the 107 screwdriver to unscrew the 2 M3X6 screws securing the main board
shielding cover, and then remove the cover (Figure 5-48).
3. Use the 107 screwdriver to unscrew the 2 M3X6 screws securing the upper shielding
cover of the signal processing board, and then remove the cover (Figure 5-48).
4. Disconnect the RBC/PLT cable connector. Refer to Section 4.2.2 for the slot
positions.
5-59
Servicing
2. Use the 107 screwdriver to unscrew the M3X6 screw fixing the RBC/PLT earth
terminal (Figure 5-49).
3. Disconnect the three fluidic lines from the RBC bath cap.
4. Use the 107 screwdriver to unscrew the 2 M3X12 screws fixing the RBC bath cap,
and then remove the cap (Figure 5-49).
5-60
Servicing
5. Disconnect other fluidic lines from the front/back bath assembly, then remove the
assembly (Figure 5-50).
6. Disconnect the fluidic and pneumatic lines from the isolation chamber, then remove
the chamber (Figure 5-50).
7. . Use the 107 screwdriver to disassemble the front/back bath assembly (Figure 5-51).
5-61
Servicing
Installation
Install the RBC bath assembly according to the steps stated above in reverse order.
Precautions
Connect the tubing as per Appendix E.
Verification
1. Check whether all components are installed and fixed in place.
5-62
Servicing
Tools
107 cross-headed screwdriver
Removal
Follow these two procedures to disassemble the premix bath assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
4. Disconnect the two fluidic lines from the premix bath cap.
5. Unplug the fluidic line connecting the premix bath to the isolation chamber.
6. Use the 107 screwdriver to loosen the 2 M4X8 screws securing the premix bath
assembly, and then remove the assembly (Figure 5-52).
7. Disconnect the fluidic/pneumatic lines from the isolation chamber, and then remove
the chamber (Figure 5-52).
5-63
Servicing
Use the 107 screwdriver to disassemble the premix bath assembly (Figure 5-53).
Installation
Install the premix bath assembly according to the steps stated above in reverse order.
5-64
Servicing
Precautions
Connect the tubing as per Appendix E.
Verification
1. Check whether all components are installed and fixed in place.
5-65
Servicing
5.7.1 Purpose
Follow the procedures to replace the HGB bath assembly.
5.7.2 Tools
101 cross-headed screwdriver
107 cross-headed screwdriver
5.7.3 Removal
Follow these two procedures to disassemble the HGB bath assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
3. Remove the outer shielding box of RBC unit as per To remove the outer shielding
box under Section 5.6.1.
4. Remove baffle plate 1 and 2 as per To remove the baffle plate 1 and 2 under
Section 5.6.1.
5. Remove the main board shielding cover as per To disconnect the RBC/PLT cable
connector under Section 5.6.1.
6. Disconnect the HGB cable connector. Refer to Section 4.2.2 for the connector
positions.
8. Disconnect the three fluidic lines from the HGB bath cap.
9. Unplug the fluidic line connecting the HGB bath to the isolation chamber.
10. Use the 107 screwdriver to loosen the 2 M4X8 screws fixing the HGB bath assembly,
and then remove the assembly (Figure 5-54).
11. Disconnect the fluidic and pneumatic lines from the isolation chamber, and then
remove the chamber (Figure 5-54).
5-66
Servicing
1. Use the 101 screwdriver to unscrew the ST2.2X10 screw securing the bracket
assembly, and then remove the assembly (Figure 5-55).
2. Use the 107 screwdriver to unscrew the 3 M3X12 screws fixing the HGB cap, then
remove the cap and bath (Figure 5-55).
5-67
Servicing
5.7.4 Installation
Install the HGB bath assembly according to the steps stated above in reverse order.
Precautions:
Connect the tubing as per Appendix E.
5-68
Servicing
5.7.5 Verification
1. Check whether all components are installed and fixed in place.
5-69
Servicing
5.8.2 Toolss
107 cross-recessed screwdriver
5.8.3 Removal
Follow these two procedures to disassemble the WBC bath assembly.
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
3. Disconnect the three pneumatics lines from the pinch valve unit.
4. Use the 107 screwdriver to unscrew the 2 M4X8 screws securing the pinch valve unit
(Figure 5-56).
6. Disconnect the connectors of the heating pipe, temperature switch and temperature
sensor from the WBC bath unit.
7. Disconnect the motors and optical sensors connector from the mixing unit.
9. Use the 107 screwdriver to unscrew the 4 M4X8 screws securing the bath unit, and
then remove the WBC bath unit and the mixing unit (Figure 5-56).
5-70
Servicing
1. Use the 107 screwdriver to disassemble the pinch valve unit as per Figure 5-57.
5-71
Servicing
2. Use the 107 screwdriver to loosen the 4 M3X6 screws securing the mixing unit, then
remove the unit (Figure 5-58).
3. To remove the two photocoupler brackets, use the 107 screwdriver to unscrew the 4
M3X6 screws securing the photocoupler brackets, and then remove the brackets
5-72
Servicing
(Figure 5-59).
4. To remove the two optical sensors, use the 107 screwdriver to unscrew the 2 M4X10
screws securing the optical sensors, and then remove the sensors (Figure 5-59).
5. The remaining part of the mixing unit is an integral part and not required to be
disassembled (Figure 5-59).
6. Use the 107 screwdriver to unscrew the 4 M3X6 screws securing the mounting plate,
and then remove the plate (Figure 5-60).
7. The remaining part of the WBC bath unit is an integral part and not required to be
disassembled (Figure 5-60).
5-73
Servicing
5.8.4 Installation
Install the WBC bath assembly according to the steps stated above in reverse order.
Precautions:
Connect the heating pipe, temperature switch, temperature sensor, motors and sensors to the
cables indicated below. Refer to Figure 5-59 and Figure 5-60 for positions of these
components on the WBC bath assembly.
5-74
Servicing
5.8.5 Verification
1. Check whether all components are installed and fixed in place.
5-75
Servicing
5.9.2 Toolss
104S-#1(38) cross-recessed screwdriver
Diagonal cutting pliers
5.9.3 Removal
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
3. Disconnect the connectors of the heating pipe, temperature switch and temperature
sensor from the reagent preheating assembly.
4. Cut the cable ties on the 8 adapters of the reagent preheating assembly using the
diagonal cutting pliers, and then remove the 8 adapters.
5. Use the 104S-#1(38) screwdriver to unscrew the 2 M4X16 screws securing the
reagent preheating assembly, and then remove the assembly (Figure 5-61).
5-76
Servicing
5.9.4 Installation
Install the reagent preheating assembly according to the steps stated above in reverse order.
Precautions:
1. Connect the heating pipe, temperature switch and temperature sensor to the cables
indicated below. Refer to Figure 5-61 for positions of these components on the
reagent preheating assembly.
5.9.5 Verification
1. Check whether all components are installed and fixed in place
5-77
Servicing
5.10.2 Toolss
107 cross-recessed screwdriver
1.3mm hexagon wrench
1.5mm hexagon wrench
2mm hexagon wrench
2.5mm hexagon wrench
5mm hexagon wrench
150mm monkey wrench
5.10.3 Removal
Follow these four procedures to disassemble the open vial sampling/SRV assembly.
To remove the open vial sampling/SRV assembly from the main unit:
1. Start up the analyzer and empty the fluidic system. Power off the analyzer and unplug
the power cord from the wall outlet.
4. Disconnect the optical sensor and step motor connector from the open vial
sampling/SRV assembly.
5. Disconnect the two pneumatic lines from the open vial sampling/SRV assembly.
6. Disconnect all fluidic lines from the open vial sampling/SRV assembly.
7. Use the 107 screwdriver to unscrew the 4 M4X8 screws securing the open vial
sampling/SRV assembly, and then remove the assembly (Figure 5-62).
5-78
Servicing
1. Use the 2.5mm wrench to unscrew the 4 M3X12 screws fixing the wipe block, and
then remove the block and the wipe (Figure 5-63).
2. Use the 2.5mm wrench to unscrew the 4 M3X8 screws fixing the SRV assembly, and
then remove the assembly (Figure 5-63).
5-79
Servicing
1. Use the 107 screwdriver to unscrew the M4X12 screw securing the optical sensor,
and then remove the sensor (Figure 5-64).
2. Use the 107 screwdriver to unscrew the 2 M3X8 screws securing the light screen, and
then remove the light screen (Figure 5-64).
3. Use the 2mm wrench to unscrew the 4 M4X8 screws securing the locating plate, and
then remove the plate (Figure 5-64).
4. Use the 5mm wrench to unscrew the 2 M6x16 screws securing the cylinder, and then
remove the cylinder (Figure 5-64).
5. Disconnect the connectors from the cylinder using the 150mm wrench (Figure 5-64).
5-80
Servicing
6. Use the 107 screwdriver to unscrew the 2 M3X8 screws securing the belt clip, and
then remove the clip (Figure 5-65).
7. Use the 2.5mm wrench to unscrew the 4 M3X5 screws securing the base, and then
remove the base (Figure 5-65). The guide rod is jointed to the base and is not
necessary to be removed.
5-81
Servicing
8. Use the 107 screwdriver to unscrew the 4 M3X8 screws fixing the step motor, then
remove the belt and the motor (Figure 5-66).
9. Use the 1.5mm wrench to unscrew the 2 M3X5 screws securing the synchronous
pulley, and then remove the pulley (Figure 5-66).
10. Use the 107 screwdriver to unscrew the 2 M3X8 screws securing the synchronous
pulley to the pulley axis, then remove the pulley and the axis (Figure 5-66).
5-82
Servicing
11. Use the 2.5mm wrench to unscrew the 4 M3X8 screws securing the linear rolling
guide, then remove the rolling guide (Figure 5-66).
z When installing the linear rolling guide, first secure the upper stop screw
and then lean the rolling guide aside.
z Adjust the tension of the synchronous belt by tightening the 4 M3X8 screws
that secure the step motor.
z When installing the synchronous pulley of the step motor, keep the motor
axis protruding 3mm from the pulley face.
12. Use the 2.5mm wrench to unscrew the 2 M3X5 screws that stops the rolling guide
(Figure 5-66).
5-83
Servicing
3. Remove the sampling valve, rotor valve and rear fixed valve in sequence (Figure
5-67).
5-84
Servicing
4. Disassemble the spring unit using the 2.5mm wrench (Figure 5-68).
5-85
Servicing
5. Use the 1.3mm wrench to unscrew the 2 M2.5X4 screws securing the sample probe,
and then remove the spacer (Figure 5-69).
7. Remove the sample probe and the sealing tube (Figure 5-69).
5-86
Servicing
8. Remove other components from the SRV assembly using the 107 screwdriver and
2.5mm wrench (Figure 5-70).
5-87
Servicing
5.10.4 Installation
Install the open vial sampling/SRV assembly according to the steps stated above in reverse
order.
Precautions:
1. Adjust the upper/lower locating plates of the cylinder rod after installing the related
components.
2. To adjust the position of the lower locating plate: (Figure 5-71 left):
5-88
Servicing
5-89
Servicing
Push the cylinder rod upwards until it stops, move the lower locating plate until it attaches the
deflector rod, then secure the lower locating plate.
To adjust the position of the upper locating plate: (Figure 5-71 right):
1. Insert the cylinder regulator into the upper locating hole on the sampling valve, and
then rotate the deflector rod to enable the cylinder regulator to enter the locating hole
of the rotor valve.
2. Push the cylinder rod downwards until it stops, move the upper locating plate until it
attaches the deflector rod, then secure the upper locating plate.
After installing the wipe block to the main unit, power on the analyzer and follow the
procedures to adjust the position of the wipe block:
1. Log on the software using the service level username and password (Figure 5-73).
5-90
Servicing
3. The Service screen shows. CLICK the Debug key at the lower right corner of the
screen (Figure 5-75).
5-91
Servicing
4. The Debug screen shows. CLICK the Wipe Block key (Figure 5-76).
5-92
Servicing
6. CLICK the Initialization key. The wipe block bracket moves to the lower position
and then to the upper position. The reference position of the wipe block is determined.
7. CLICK the Middle place key. The wipe block moves to the middle position. CLICK
the Hold Moment key. The motor axis is locked.
8. Insert the height gauge into the wipe block until attaching the bottom of the wipe
(Figure 5-79). Slowly unscrew the 4 M3X12 screws securing the fixing block, move
the wipe block up and down to enable the sample probe to exactly attach the top end
of the height gauge, and then secure the 4 screws.
10. Exit the wipe block debugging screen. The analyzer performs initialization
automatically (Figure 5-78).
11. After adjusting the wipe block position, you can proceed to other operations.
5-93
Servicing
13. Connect the optical sensor and step motor to the cables indicated below:
5-94
Servicing
5.10.5 Verification
1. Check whether all components are installed and fixed in place.
5-95
Servicing
5.11.2 Tools
101 cross-recessed screwdriver
107 cross-recessed screwdriver
1.5mm hexagon wrench
2.5mm hexagon wrench
3mm hexagon wrench
5.11.3 Removal
The sample transmitting assembly can be removed in these two procedures:
1. Remove the sample transmitting assembly from the analyzer. (See Section 5.2.5 for
details).
2. Use the 3mm wrench to unscrew the two M4X6 screws fixing the sample transmitting
connection blocks and then remove the two blocks (Figure 5-80).
5-96
Servicing
4 3 2 1
1. Use the 107 screwdriver to unscrew the two cross-recessed screws fixing the
transmitting assembly cover and the 2 M3X8 screws fixing the scanner, and then
remove the cover (Figure 5-81).
5-97
Servicing
2. Pull out the connector of the fixed barcode scanner, use the 107 screwdriver to
unscrew the 4 little panhead screws M3X8 fixing the scanner bracket and the scanner
on the back supporting plate unit, and then remove the scanner bracket and the
scanner (See Figure 5-82 and Figure 5-83).
5-98
Servicing
5-99
Servicing
3. Use the 107 screwdriver to unscrew the 4 M3X8 screws fixing the elastic presser and
the barcode scanner on the riser unit, and then remove the presser and the barcode
scanner (See Figure 5-82 and Figure 5-84).
5-100
Servicing
4. Pull out the photocoupler (reflection) connector, use the 107 screwdriver to unscrew
the two M3X8 screws fixing the back supporting plate unit and then remove the unit
(See Figure 5-82 and Figure 5-83).
5. Use the 101 screwdriver and the 107 screwdriver to disassemble the rest parts on the
back supporting plate unit according to Figure 5-83.
6. Use the 107 screwdriver to unscrew the two M3X8 screws fixing the riser unit and
then remove the unit (Figure 5-82 and Figure 5-84).
7. Use the 107 screwdriver to disassemble the rest parts on the riser unit according to
Figure 5-84.
8. Use the 107 screwdriver to unscrew the two M3X8 screws fixing the front supporting
plate and then remove the board (Figure 5-82 and Figure 5-85).
9. Pull out the connector of sensitive switch on the deck unit and the connector of
photocoupler (reflection), use the 107 screwdriver to unscrew the 5 M3X8 screws
5-101
Servicing
fixing the deck unit and then remove the unit (see Figure 5-82 and Figure 5-85).
10. Use the 101 screwdriver and the 107 screwdriver to disassemble the deck unit
according to Figure 5-85. Note: Do not disassemble the sponge baffle.
11. Use the 107 screwdriver to unscrew the 2 M3X8 screws fixing the unloading unit, pull
out the connector of optical sensor on the unloading unit, and then remove the
unloading unit (see Figure 5-82 and Figure 5-86).
12. Use the 107 screwdriver to disassemble the unloading unit according to Figure 5-86.
5-102
Servicing
13. Pull out the connector of the stepping motor on the feeding unit and the connectors of
the 3 optical sensors, use the 107 screwdriver to unscrew the 3 M3X8 screws fixing
the feeding unit, and then remove the feeding unit (see Figure 5-82 and Figure 5-87).
14. Use the 1.5mm wrench, 2.5mm wrench and the 107 screwdriver to disassemble the
feeding unit according to Figure 5-87.
5-103
Servicing
15. Pull out the connector of the stepper motor on the loading unit and the connectors of
the two optical sensors, use the 107 screwdriver to unscrew the 4 M3X8 screws fixing
the loading unit, and then remove the unit Figure 5-82 and Figure 5-88).
16. Use the 1.5mm wrench, 2.5mm wrench, the 101 screwdriver and the 107 screwdriver
to disassemble the loading unit according to Figure 5-88.
5-104
Servicing
8 9 10 11 12 13
7 14
15
6
5 16
4 17
3 18
2 19
20
1
21
25 24 23 22
Figure 5-88 Disassembling the autoloader-8
5-105
Servicing
17. Use the 107 screwdriver to unscrew the 4 M3X8 screws fixing the sample transmitting
adapter board and then remove the board (Figure 5-89).
5.11.4 Installation
Install the autoloader assembly according to the removal procedures stated above to in
reverse order.
Precautions and position adjustment during the installation:
Lean the guide rail against one side. Note that the stop screw should be installed
first.
Before assemble the synchronous pulley, grease the surface of the driven pulley
shaft.
After the synchronous pulley of the stepper motor is installed, level the end of the
motor shaft with the end surface of the synchronous pulley.
When installing the stepper motor, adjust the extension of the synchronous belt
before tightening the fixing screw.
5-106
Servicing
Before assembling the synchronous pulley, grease the surface of the driven pulley
shaft.
After the synchronous pulley of the stepper motor is installed, level the end of the
motor shaft with the end surface of the synchronous pulley.
When installing the stepper motor, adjust the extension of the synchronous belt
before tightening the fixing screw.
Make sure the teeth bars can move smoothly after the installation. If the resistance is
great, repair them as necessary, otherwise the unit may not be repositioned.
1) Start up the analyzer and go to the "Service" screen. CLICK the Debug button at the
lower right corner of the screen. At the Debug screen, CLICK the "Mix Unit" button to go to
the "Mix Unit" screen shown in Figure 5-91.
5-107
Servicing
2) At the "Mix Unit" screen, CLICK "Initialization" in the "Autoloader" area to initialize the
autoloader; then CLICK "Unload" to complete the initialization of the feeding motor, as shown
in Figure 5-92. Note: if the buttons gray out, CLICK the "Open compressor" button. After the
compressor is open, the buttons will be activated.
5-108
Servicing
3) Loosen the feeding and loading/unloading adjusting screws in turn, and then make
adjustment as per Figure 5-93: the distance between the short teeth bar of the unloading unit
and the sample transport platform shall be 0.5mm, and that between the long teeth bar and the
upper buffle plate of the feeding slider shall be 1mm. Secure the adjusting screws after the
adjustment.
4) Fill up the loading tray with tube racks (make sure the racks are placed close to both sides
of the loading tray). At the mix unit debug screen, CLICK the "Unload" button in the
"Autoloader" area to unload, and then check the position of the unloaded tube rack to see if
the next tube rack can be unloaded properly, as shown in Figure 5-94.
While installing the barcode scanner, make sure the beam is emitted in the center of
the barcode on the tube. Otherwise, adjust the position of the barcode scanner.
2. Adjusting the position of the front and back supporting plates of the autoloader with
5-109
Servicing
the fixture
Press the installation fixture firmly against the inside of the transport platform, and
slide it along to where the back boards are installed, as shown in Figure 5-95. Make
sure you do not place the fixture reversedly.
Install the front supporting plate: move the front supporting board close to the fixture
as shown in Figure 5-96 and Figure 5-97, and then mount the screws. Leave the first
screw not tightly secured; secure the second screw first, and then tighten the first
one.
z While securing the second screw, press the front supporting plate firmly
against the fixture, and make sure the fixuture cling to the transport
platform.
5-110
Servicing
Figure 5-96 Finding the proper position to install the front supporting board
Install the back supporting plate: adjust the position of the back supporting plate as
shown in Figure 5-98 and Figure 5-99, and then mount the screws. Leave the first
screw not tightly secured; secure the second screw first, and then tighten the first
one.
z While securing the second screw, press the front supporting plate firmly
against the fixture, and make sure the fixuture cling to the transport
platform.
5-111
Servicing
After adjusting the front and back supporting plates, pull the tube rack to through the
passage between the supporting boards to check if it can get through smoothly, If not,
readjust the supporting plates.
1. For the adjustment of the manipulator, see Heading 7.3 Adjusting Positions of
BC-5800&5600 Auto Hematology Analyzer Instalation Guide.
2. See Figure 5-100 for the position and connection of sensors and stepper motors.
5-112
Servicing
5-113
Servicing
5.11.5 Verification
1. Check whether each part is assembled and tightened well.
5-114
Servicing
5.12.2 Toolss
107 cross-recessed screwdriver
1.5mm hexagon wrench
2.5mm hexagon wrench
4mm hexagon wrench
6mm hexagon wrench
150mm monkey wrench
5.12.3 Removal
The autosampler and mixer assemblies can be removed in three procedures
1. Startup the analyzer to empty the fluidic system, and then shutdown and disconnect
the power cord of the analyzer.
2. Open the lower part of the cover (see Section 5.2.5 for details).
3. Pull out the seven optical sensor connectors and one stepper motor connector from
the two assemblies.
5. Pull out the 3 fluidic tubes from the wipe block unit of the autosampler assembly, and
loosen the nut at the end of the pierce needle.
6. Use the 107 screwdriver to unscrew the four M4X8 screws fixing the two assemblies
and then remove the piercing and mixing assembly (Figure 5-101).
5-115
Servicing
7. Use the 107 screwdriver to unscrew the five M4X8 screws fixing the autosampler
assembly and then remove the assembly (Figure 5-102).
5-116
Servicing
1. Unscrew the two set screws and remove the cover (Figure 5-103).
2. Use the 107 screwdriver to unscrew the two M4x20 screws, use the 2.5mm wrench to
unscrew the four M3x8 screws and then remove the wipe block unit (Figure 5-103).
5-117
Servicing
3. Use the 107 screwdriver and the 150mm wrench to disassemble the wipe block unit
according to Figure 5-104.
5-118
Servicing
4. Use the 107 screwdriver, the 2.5mm wrench, the 6mm wrench and the 150mm
wrench to disassemble the rest parts of the autosampler according to Figure 5-105.
5-119
Servicing
15 16 17 18
14 19
13
12
11
10
20
9
8
7 21
6
5 22
4
3
5-120
Servicing
1. Use the 107 screwdriver to unscrew the M4X10 screw fixing the optical sensors and
then remove the six optical sensors (Figure 5-106).
2. Use the 150mm wrench to unscrew the cylinder nut and the connection bushing that
fix the cylinder and then remove the cylinder (Figure 5-106).
3. Use the 150mm wrench to unscrew the tube connector on the cylinder (Figure 5-106).
4. Use the 2.5mm wrench to unscrew the M3X12 screw that fixes the swinging arm, and
then remove the arm (Figure 5-106).
5. Use the 107 screwdriver to unscrew the M3X8 screw that fixes the pinchers, and then
remove the pinchers (Figure 5-106).
6. Use the 107 screwdriver to unscrew the four M3X8 screws that fix the stepper motor,
and then remove the motor (Figure 5-106).
7. Use the 1.5mm wrench to unscrew the M3X5 screw that fixes the induction block, and
then remove the block (Figure 5-106).
10
8
7 11
6 12
5 13
4 14
3
15
2 16
1 17
5-121
Servicing
8. Use the 150mm wrench to unscrew the cylinder nut and the connection bushing that
fix the cylinder and then remove the cylinder (Figure 5-107).
9. Use the 150mm wrench to unscrew the tube connector of the cylinder (Figure 5-107).
10. Use the 4mm wrench to unscrew the two M5x6 screws fixing the cylinder bracket and
then remove the bracket (Figure 5-107).
11. Use the 107 screwdriver to unscrew the two M4x8 screws fixing the connection bent
plate and then remove the plate (Figure 5-107).
5-122
Servicing
12. Use the 2.5mm wrench to disassemble the rest parts of the mixing module according
to Figure 5-108.
5-123
Servicing
5.12.4 Installation
Install the autosampler and auto-mixer assemblies according to the removal procedures stated
above to in reverse order.
Precautions and position adjustment during the installation:
1. Lean the linear guide rail against one side. Note that the stop screw should be
installed first.
2. Make sure the moving unit can move upward and downward/forward and backward
smoothly.
3. After the installation, adjust the initial position of the pincher: Unscrew the two locking
nuts on the front end of the cylinder rods and adjust the connection bushings of
cylinders to fix the pincher position (See Figure 5-109 for how to adjust the pincher).
Then tighten the locking nuts.
5-124
Servicing
4. Adjust the left and right initial position of the pincher (Figure 5-110).
Unscrew the locking screws on the swinging arm, startup the analyzer (you may ignore the
mixing motor error reported), enter the Service Debug Mix unit screen, and CLICK
the Init. button for three times so that the swinging arm is on the vertical position. Lean the
swinging arm on the stop baffle to the right, place several pieces of paper between the arm
and the baffle, and then tighten the swinging arm. Initialize the mixing motor again to check the
swinging arm position. The swinging arm should move from the initial position to the stop
position and then return the initial position. When the arm is on the initial position, there should
also be tiny clearance between the swinging arm and the stop baffle so that the arm would not
touch the baffle. If not, repeat the procedures above until the position is proper.
7
6
5
5-125
Servicing
After the installation, the autosampler assembly needs to be adjusted to make sure that the
axis deviation of the pierce needle and the sample tube is no greater than 2mm in the diameter
direction. The five holes on the assembly bracket are used to adjust the forward and backward
direction of the assembly, and the four big holes and the pad on the assembly installation bed
are used to adjust the up and down/left and right/around position of the assembly. After the
adjustment, glue and then lock the fixing screws.
Parallel the tube to the pierce needle: Push a tube rack manually on the Y feeding direction,
place an empty collection tube in the clipping position (the position leveling the mixing motor),
enter the Service Debug Mix unit screen, and then CLICK the Mix button to
rotate the tube to the sampling position. Use a ruler to check the parallel of the tube and the
pierce needle in the front and side of the tube. If they are not parallel, adjust the relevant
screws until you see that they are parallel.
Adjust to make the tube and the pierce needle coaxial: Remove the cap and plug from an
empty tube, use a gummed paper to envelop the tubing opening and place the tube into the
rack. Enter the Service Debug Mix unit screen, CLICK the Sample&Mix&pierce
button to pierce the tube, and then CLICK the Return tube button to return the tube to the
rack. Adjust the autosampler unit position through the piercing hole. Repeat the procedures
above if necessary until the piercing hole is in the center of the tube opening.
5-126
Servicing
6. Adjust the wipe block (Figure 5-112). Loosen the 4 fixing screws of the connecting rail
block on the wipe block bed (need not remove the screws), place the locating piece
between the wipe block and the cylinder bed, press the wipe block onto the locating
piece, and then secure the 4 screws and remove the locating piece. Ensure that the
press plate at the bottom of the wipe block is not pressed by the locating piece.
5-127
Servicing
7. You can adjust the manual control screw at the end of the cylinder to control the
piercing depth. See procedures below (Figure 5-113).
Place a standard tube (13mm*81.2mm) in the tube rack. Enter the Service Debug
Mix unit screen, CLICK the Sample&Mix&pierce button, and then check if the distance
from the wipe pedestal to the piercing needle fixing pedestal is 1-2mm. If not, adjust the
cylinder stroke knob, and then secure the cylinder stroke retainer ring, as shown in Figure
5-113.
9. See Figure 5-114 for the position and connection of the sensors and motors.
5-128
Servicing
5.12.5 Verification
1. Check whether each part is assembled and tightened well.
3. Check whether the air tubes and fluidic tubes are connected correctly.
4. Startup the analyzer to check the movement of the auto sampler and auto-mixer
assemblies.
5-129
Servicing
5.13.2 Tools
101 slotted head screwdriver
107 cross-recessed screwdriver
300mm monkey wrench
Nipper pliers
3mm hexagon wrench
5.13.3 Removal
1. Power off the analyzer. Disconnect the power cord, control line of the pneumatic
control board and pressure/vacuum lines from the pneumatic unit.
2. Use the 107 screwdriver to unscrew the 8 M3X6 screws securing the pneumatic unit
cover, then remove the cover (Figure 5-115).
5-130
Servicing
3. Disconnect the 6 pneumatic lines from the vacuum pump, release valve and screwed
connectors (Figure 5-116).
4. Use the 107 screwdriver to unscrew the 2 M3X6 screws fixing the shielding cover of
the pneumatic unit control board, and then remove the shielding cover.
5. Disconnect all connectors from the pneumatic control board and 3 connectors from
the power filter.
6. Use the 107 screwdriver to unscrew the M4X10 screws securing the two earth wires
(Figure 5-117).
7. Use the 107 screwdriver to unscrew the 2 M3X6 screws securing the power filter, then
remove the filter (Figure 5-117).
8. Use the 300mm wrench to unscrew the retaining screw securing the release valve,
then remove the valve (Figure 5-117).
9. Use the nipper pliers to unscrew the 2 screws fixing the control line of the interface
board/pneumatic control board, then remove the line (Figure 5-117).
10. Use the 107 screwdriver to unscrew the 4 M3X6 screws securing the upper spacer,
then remove the spacer (Figure 5-117).
5-131
Servicing
11. Use the 107 screwdriver to unscrew the 4 M3X6 screws securing the pneumatic
control board, then remove the board (Figure 5-118).
12. Use the 3mm wrench to unscrew the 4 M4X6 screws fixing the fan, then remove the
fan (Figure 5-118).
13. Use the 107 screwdriver to unscrew the 2 M3X6 screws securing the temperature
sensor, then remove the sensor (Figure 5-118).
5-132
Servicing
14. Remove the fuse tubes by prizing the tube bracket with the 101 screwdriver (Figure
5-119).
5-133
Servicing
15. Lift up the vacuum pump to release the studs, then remove the vacuum pump (Figure
5-120).
16. Unscrew the stud from the base plate of the air compressor using the 300mm wrench
(Figure 5-120).
17. Use the 107 screwdriver to unscrew the 8 M5X10 screws securing the base plate and
guide rod, then remove the base plate and guide rod (Figure 5-120).
18. Use the 107 screwdriver to unscrew the 8 M3X6 screws securing the lower spacer,
then remove the spacer (Figure 5-120).
19. Use the 107 screwdriver to unscrew the 2 M4X8 screws securing the capacitor, then
remove the capacitor (Figure 5-120).
5-134
Servicing
20. Use the 3mm wrench to unscrew the 4 M4X6 screws fixing the fan, then remove the
fan (Figure 5-121).
21. Use the 107 screwdriver to unscrew the 4 M5X10 screws fixing the guide rod, then
remove the rod (Figure 5-121).
22. Remove the remaining parts from the pneumatic unit using the 107 screwdriver and
300mm wrench (Figure 5-122).
5-135
Servicing
5.13.4 Installation
Install the pneumatic unit according to the steps stated above in reverse order.
Precautions:
5. Connect cables to the three slots on the power filter (Figure 5-123).
5-136
Servicing
5.13.5 Verification
1. Check whether all components are installed and fixed in place.
5. Forced startup of the pneumatic unit: use a solder stick or iron wire to short-circuit Pin
2 and 8 of the control cable socket, and the pneumatic unit will be started up.
5-137
Servicing
5.14.1 Purpose
Follow the procedures to replace the temperature sensor.
5.14.2 Tools
107 cross-recessed screwdriver
5.14.3 Removal
1. Power off the analyzer and unplug the power cord from the wall outlet.
3. Disconnect the J32 electric connecter from the driver board as Figure 5-125 shows.
4. Use the 107 screwdriver to unscrew the 4 M3X12 screws securing the fixing support,
and then remove the fixing support, ambient temperature sensor and connecting line
as Figure 5-125 shows.
5-138
Servicing
5.14.4 Installation
Install the temperature sensor according to the steps stated above in reverse order.
5.14.5 Verification
1. Check whether all components are installed and fixed in place.
5-139
Servicing
5.15.1 Purpose
Because there will be something wrong with the blood sensor when it is used, it should
be replaced during maintenance.
5.15.2 Tools
101 cross-recessed screwdriver
107 cross-recessed screwdriver
5.15.3 Removal
1. Open the top cover (See Section 5.2.4 );
5. Loosen and take away the two pan head screws with cross recess M3X6 with 107
cross screwdriver and take away the blood sensor (Figure 5-126)
5-140
Servicing
5.15.4 Installation
Reverse the removal procedures to install it.
Note:
5.15.5 Verification
1. Check whether all components are installed and fixed well.
4. In auto-sampling mode, continuously make once normal sample test and blank
background test. If Insufficient aspiration is not prompted of the normal sample,
but Abnormal sample or Insufficient aspiration can be prompted in
background test, then the replacing is normal.
5-141
Servicing
5.16.2 Tools
104S-#1(38) cross-recessed screwdriver
4mm hexagon wrench
Nipper pliers
5.16.3 Removal
1. Disconnect the power cord of the analyzer.
3. Pull out the 2 gas hoses from the inlet and outlet of the filter and the draining tube at
the bottom.
4. Use the 104S-#1(38) cross-recessed screwdriver to unscrewn the M4X8 screw fixing
the filter.
6. Loosen the top cap of the filter, and disassemble the cap and the filter bowl (as shown
in Figure 5-127).
7. Use the nipper pliers to loosen (tweak counterclockwise) the manger board (as shown
in Figure 5-128)
5-142
Servicing
Top cap
Filtering
cartridge
Filter bowl
Manger board
5-143
Servicing
Guide plate
5.16.4 Installation
1. Install a new filtering cartridge, and then secure the guide plate.
2. Secure the manger board to the top cover by tweaking it clockwise with the nipper
pliers.
z Make sure you install the top cap properly as indicated by the arrows on the
cap.
5.16.5 Verification
1. Check whether all components are installed and fixed in place.
2. Check whether the gas hoses are connected correctly.
3. Power on the analyzer and verify your replacement.
5-144
6 Optical System
Purpose
Replace and repair critical erroneous parts of the optical system.
Tools
Antistatic gloves
Hexagonal wrench
Cross-headed screwdriver
Straight slot screwdriver
Horologic screwdriver
Duke (4K-07) 7um standard particles
RD calibrator, control or RD-5D control (choose only one kind depending on specific
situations)
Oscilloscope or oscillometer
z Disposable dust-free gloves must be worn for all operations of the optical
system.
6-1
Optical System
The optical system can be divided in to 6 parts: front light assembly, flow cell assembly, rear
light assembly, light splitting assembly, rear light collecting assembly and rear light detecting
assembly.
z Please make sure that power is off and power cord is disconnected when
removing and installing boards.
z Do not plug or unplug laser semiconductor before power is off, or the laser
semiconductor can be damaged.
1. Turn off the power, open the cover of the laser and take the connection wire off the
driver board
6-2
Optical System
2. Loosen the fixing screw 1 and take off the support of the laser driver board.
3. Loosen screw 2, take off the laser driver board and replace it with a new one, and
then install the new laser driver board and fix the screws.
1--Fixing screws of the laser driver board 2--Fixing screws of the laser driver board
support
2. Unplug the laser power plug on the laser control board, disconnect plugs of the
heater, the temperature sensor and the overheat protection switch.
3. Loosen the fix screws on both sides of the front light assembly with the hexagonal
wrench, and take off the front light assembly.
4. The front light assembly is adjusted by special tools, so users are only allowed to
remove the assembly from the base plate without dismantling any component or
sub-assembly in it.
6-3
Optical System
5. If the error of the front light assembly is confirmed, the assembly needs to be
replaced.
6. Finally connect the laser power plug and the plugs of the heater, temperature sensor
and over-heat protection switch.
1. Empty fluidic tubing when starting up the analyzer, and then power it off and
disconnect its power cord;
2. Loosen the two M4 screws fixing the rear light assembly to the base plate, then
remove the entire rear light assembly;
3. Pull out the sheath fluid inlet tube, sample inlet tube and the waste outlet tube (at the
outlet of the flow cell); loosen the four M3x10 screws (with washer) with
cross-headed screwdriver, and then loosen the two M3x10 screws (with washer)
fixing the flow cell tray, finally take out the flow cell assembly. See Figure 6-4.
6-4
Optical System
z Do not bend the three-way connector when dismantling the flow cell.
4. Loosen the two connecting screws with the hexagonal wrench; the flow cell then
becomes two parts: rectifier assembly and detection assembly. See Figure 6-5.
5. The detection assembly and the fluid transport assembly need to be cleaned and
replaced according to specific situations.
6-5
Optical System
6. Install the flow cell assemblies per the above procedure in reverse order.
2. Loosen the fix screws on both sides of the rear light assembly with the hexagonal
wrench, and take off the rear light assembly.
3. Loosen the two fixing screws on the diaphragm lens assembly with the hexagonal
wrench, and then take off the assembly.
4. Replace the rear light diaphragm lens assembly, and install it to the rear light
orienting assembly to form a new rear light assembly. Please note that the
diaphragm lens assembly and the rear light orienting assembly must be in precise
seamless contact. Then install the rear light collimation assembly to the optical base
plate and fix it. Please note that before installing new rear light assembly, you need to
clean the surfaces of all assemblies with dust-free cloth and absolute alcohol, and
wait until the surfaces get dry.
6-6
Optical System
2. Loosen the fix screws on both sides of the light splitting assembly with the hexagonal
wrench, and take off the assembly.
3. Remove the two clamping rings with the straight slot screwdriver; loosen the screws
on the top plate and the side plates with the cross-headed screwdriver to remove the
top and side plate.
4. Remove the two protective gaskets; take off the light-splitting lens carefully.
5. When Replacing the light splitting lens, you need to pay attention to the direction of
the lens (see Figure 6-7). Finish installation per the removing procedure in reserve
order.
LAS assembly
2. Loosen the two fixing screws of the LAS assembly with hexagonal wrench, and take
the assembly off from the optical base plate.
3. Loosen the two screws fixing the diaphragm with the cross-headed screwdriver,
remove the low-angle diaphragm; loosen the two screws fixing the rear light focus
6-7
Optical System
4. Replace and then fix the low-angle diaphragm and the rear light focus lens assembly
according to specific situations.
5. Install the LAS assembly to the optical base plate and fix it with the hexagonal
wrench. Make sure that the diaphragm assembly is close to the M4 hexagonal screw
when fixing the assembly.
MAS assembly
2. Loosen the two fixing screws of the MAS assembly with hexagonal wrench, and take
the assembly off from the optical base plate.
3. Loosen the two screws fixing the diaphragm with the cross-headed screwdriver,
remove the diaphragm; loosen the two screws fixing the rear light focus lens
assembly with the screwdriver and remove the assembly.
6-8
Optical System
4. Replace and then fix the diaphragm and the rear light focus lens assembly
according to specific situations.
5. Install the MAS assembly to the optical base plate and fix it with the hexagonal
wrench. Make sure that the diaphragm assembly is close to the M4 hexagonal screw
when fixing the assembly.
Low-angle PD assembly
2. Loosen the two fixing screws of the low-angle PD assembly with hexagonal wrench,
and take the assembly off from the optical base plate.
3. Loosen the two screws fixing the shielding cover with the cross-headed screwdriver,
and take the shielding cover off from the low-angle PD assembly.
6-9
Optical System
4. Remove the low-angle pre-amplification board from the PD assembly with the
cross-headed screwdriver, then remove PD slide, replace components and install
them per the procedure above in reserve order. Pay attention not to touch PD
surface.
5. Install the shielding cover to the low-angle PD assembly, and then install the PD
assembly to the optical base plate.
High-angle PD assembly
2. Loosen the two fixing screws of the high-angle PD assembly with hexagonal wrench,
and take the assembly off from the optical base plate.
3. Loosen the two screws fixing the shielding cover with the cross-headed screwdriver,
and take the shielding cover off from the high-angle PD assembly.
6-10
Optical System
4. Remove the high-angle pre-amplification board from the PD assembly with the
cross-headed screwdriver, then remove PD slide, replace components and install
them per the procedure above in reserve order. Pay attention not to touch PD
surface.
5. Install the shielding cover to the high-angle PD assembly, and then install the PD
assembly to the optical base plate.
6.3 Adjustment
After removing any assemblies in the optical system, the system needs to be adjusted. The
adjustment procedure can be adapted according to specific situations of removal.
The adjustment of the optical system can be classified as rough adjustment and fine-tuning.
The purpose of rough adjustment is to collimate all optical assemblies in the system to make
them on the same optic axis so that the system can generate signals quickly when the
operation sequence is detecting standard particles. The purpose of fine-tuning is to ensure
the optical system is in the optimal position. By observing signal waveform with oscilloscope,
the position of the optical assemblies can be subtly adjusted to make the generated signal
6-11
Optical System
6-12
Optical System
6-13
Optical System
Step2: install the rear light assembly, fix the assembly on the base plate using M4 hexagonal
screws, spring washer and plain washer (ensure that the rear light assembly can be moved
along the pins). Move the rear light collimating assembly horizontally along the positioning
pins and observe the position of the spot on the diaphragm. When the spot is at the center of
the diaphragm (see Figure 6-16 Horizontal adjustment of rear light collimating assembly
completes), fix the M4 hexagonal screws on the base plate.
6-14
Optical System
Step 3: Loosen the 2 M3 hexagonal screws connecting the rear light diaphragm lens
assembly and the orienting assembly slightly to pre-fixing status; see Figure 6-17. Move the
diaphragm lens assembly slightly to turn the spot on the high-angle diaphragm to the center
of the two vertical rulings on the MAS diaphragm; see Figure 6-18. The vertical adjustment of
the diaphragm lens assembly is done; the last step is to fix the screws on the diaphragm lens
assembly.
Figure 6-18 The spot on the high-angle diaphragm is at the center of the two rulings
z The rear light assembly must be close to the positioning pins during
adjustment.
Adjustment purpose: put the LAS diaphragm symmetrically with the optic axis, select
6-15
Optical System
6-16
Optical System
Put a piece of paper (with a line on it) on the MAS diaphragm, make the shadow image of the
flow cell distribute symmetrically by the line; see Figure 6-21. Adjust the direct light masking
diaphragm, when the masked spot distributes symmetrically by the line, the adjustment is
done.
After the adjustment completes, pre-fix the two screws of the direct light masking diaphragm
(see Figure 6-22).
Adjustment purpose: ensure that the PD aperture is at the center of the optical path and the
scattered light can be collected by PD completely;
Adjustment procedure: adjust the horizontal and vertical position of the low-angle PD
assembly so that the center of the spot transmitting from the LAS diaphragm can be received
6-17
Optical System
After all the above steps, the rough adjustment is almost completed, which can ensure that
each assembly is placed symmetrically by the optic axis. The fine-tuning of optical system is
described below.
6.3.2 Fine-tuning
The purpose of fine-tuning is to make sure that the optical system is at the optimal position so
that it can detect the scattered light of designed angle. Fine-tuning concerns the following
three aspects: (1) the laser beam is focused on cell flow;(2) the center of sample flow and the
spot matches together;(3) adjust rear light assembly so that it can detect scattered light of
designed angle. So the main adjustment points are the axial position of the front light system,
horizontal position of the flow cell and the position of rear light collimating assembly.
6-18
Optical System
6-19
Optical System
Figure 6-26 Signal chart of the front light system outputting standard particles
Note: The sample push cycle of the DIFF channel is 8.5s; in order to simplify adjustment
process and generate signals quickly, you can adjust the front light axial adjusting rotary knob
in advance to turn the distance between the front faces of the front light orienting assembly
and the orienting assembly base to 3mm.
3. Fine-tuning rear light collimating assembly and direct light masking diaphragm
Adjustment purpose: 1 ensure that the PD assembly detects scattered light of the designed
collecting angle.
6-20
Optical System
2 ensure the direct light masking diaphragm and the sample flow are coaxial.
Adjustment procedure:
Step 1: connect TPOUT on the pre-amplification board of high-angle PD assembly (or
connect TP44 MAS_IN on the signal processing board) to oscilloscope channel 2 (CH2); the
settings of the oscilloscope are: voltage: 100mv/div, time: 500ns/div, AC coupling.
step 3: perform normal open-vial standard particle test, adjust the rotary knob of the rear light
collimating assembly, and observe signal pulses of the oscilloscope channel 2 (i.e. high-angle
signal pulses); when the peak value of the pulses is turned to its maximum and the signals
are stable, fix the screws of the rear light collimating assembly.
Step 3: switch to channel 1 (CH1), observe whether the peak value of the signals is
200mv10mv; if it is outside the range, adjust the adjustable resistor on the laser control
board to make it fall within the range 200mv10mv.
Step 4: observe whether the peak value of channel 2 (CH2) is above 800mv; if not, adjust the
rotary knob of the rear light collimating assembly. When the peak value is adjusted to the
defined range, check whether it is at its maximum.
6-21
Optical System
6-22
Optical System
Step 1: under "Count" screen, make sure the WBC background voltage is below 0.1. (If the
WBC background voltage is outside the range, please clean the flow cell or conduct
background counting for several times).
Step 2: enter "Count" screen, select the mode "OV-WB" and "CBC+5DIFF", perform
counting for 3 times successively using calibrators, and set sample numbers to "Analyzer
No.-JZ1", "Analyzer No.-JZ2" and "Analyzer No.-JZ3".Select "Review", enter the "Table
Review" screen, select test data. click "OpAdjust" to enter into the optical adjustment
screen, select "Calibrator". Enter calibrator target value into the "CG Target" tab, calculate
the LAS and MAS gains of the 3 calibrator count cycles respectively, and then calculate the
mean of the gains and record the mean as the final gain (adopt the round-up value).
Step 3: enter "Gain" screen, set WBC_LAS (DIFF) and WBC_MAS (DIFF) to the final value
and then auto-calculate BASO gain per the pre-defined "corresponding factor of BASO and
DIFF".
Step 4: perform counting once again with calibrator. The LAS and MAS CG position must
meet requirements.
LAS CG position: target value 2.00;
MAS CG position: target value 2.00;
Step 1: under "Count" screen, make sure the WBC background voltage is below 0.1. (If the
WBC background voltage is outside the range, please clean the flow cell or conduct
background counting for several times).
Step 2: enter "Count" screen, select the mode "OV-WB" and "CBC+5DIFF", perform
counting for 3 times successively using calibrators, and set sample numbers to "Analyzer
No.-ZK1", "Analyzer No.-ZK2" and "Analyzer No.-ZK3".Select "Review", enter the "Table
Review" screen, select test data. click "OpAdjust" to enter into the optical adjustment screen,
select "Calibrator". Enter calibrator target value into the "CG Target" column, calculate the
LAS and MAS gains of the 3 calibrator count cycles respectively, and then calculate the mean
of the gains and record the mean as the final gain (adopt the round-up value).
Step 3: enter "Gain" screen, set WBC_LAS (DIFF) and WBC_MAS (DIFF) to the final value
and then auto-calculate BASO gain per the pre-defined "corresponding factor of BASO and
DIFF".
Step 4: perform counting once again with calibrator. The LAS and MAS CG position must
meet requirements.
LAS CG position: target value 2.00;
MAS CG position: target value 2.00;
6-23
Optical System
R&D control (CBC-5DMR) calibration steps (for countries and regions outside
China)
Step 1: under "Count" screen, make sure the WBC background voltage is below 0.1. (If the
WBC background voltage is outside the range, please clean the flow cell or conduct
background counting for several times).
Step 2: enter "Count" screen, select the mode "OV-WB" and "CBC+5DIFF", perform
counting for 3 times successively using calibrators, and set sample numbers to "Analyzer
No.-ZK1", "Analyzer No.-ZK2" and "Analyzer No.-ZK3".Select "Review", enter the "Table
Review" screen, select test data. click "OpAdjust" to enter into the optical adjustment
screen, select "Calibrator". Enter calibrator target value into the "CG Target" column,
calculate the LAS and MAS gains of the 3 calibrator count cycles respectively, and then
calculate the mean of the gains and record the mean as the final gain (adopt the round-up
value).
Step 3: enter "Gain" screen, set WBC_LAS (DIFF) and WBC_MAS (DIFF) to the final value
and then auto-calculate BASO gain per the pre-defined "corresponding factor of BASO and
DIFF".
Step 4: perform counting once again with calibrator. The LAS and MAS CG position must
meet requirements.
LAS CG position: target value 2.00;
MAS CG position: target value 2.00;
Sample Verification
Step 1: select samples
After set up the gain of the above standard particles, select 10 tubes of fresh anticoagulant
venous blood that meet below requirements (within 6 hours after the samples are
collected).The 10 samples must meet below two requirements:
Requirement 1: parameter requirement (parameters on the "Count" screen)
These parameters can be observed in the "Table Review" screen ("Count" screen).
If no qualified sample can be found, there are two possibilities. One is the samples of the
hospital are abnormal, for example the hospital is a hematopathy hospital or the hospital only
takes capillary blood. Under this situation, engineers need to get blood sample from other
6-24
Optical System
For samples that are qualified under Requirement 1, its scattergram form still needs to be
observed. If the scattergram form is abnormal or the algorithm differential is apparently
incorrect, then the samples are not qualified.
The scattergram of normal fresh blood is shown in below Figure 6-30:
If the WBC scattergram of normal fresh blood is different from the above figure, then the
sample is also unqualified, for example the border of cell populations is vague, the cell
populations are badly clustered or the scattergram form is loose or with many unorderly dots.
See Figure 6-31.
Some samples cannot be differentiated, the parameters of the samples are displayed as "**".
They are unqualified for Requirement 1, so their scattergrams are omitted here.
6-25
Optical System
Select "Sample Mode" under the "OpAdjust" screen and then click "Calculate", the
parameters of the two types of particles will be displayed in the white box on the right. The
two types of particles are marked with red box in the scattergram. See Figure 6-33.
Repeat the operation on the 10 samples selected from Step 1, if more than 7 samples are
qualified, the analyzer is in normal condition, otherwise it is abnormal. If deviation to the
sample direction is found in all the samples, there must be problem concerning gain setup or
adjustment. Please reset the gain of or fine-tune the optical system.
Particle 1 (the particle in the red box on the lower left side of the scattergram) requirements:
Particle 2 (the particle in the red box on the upper right side of the scattergram) requirements:
2. If after calibration, the fresh blood CG range requirements still cannot be met,
6-26
Optical System
fine-tune the optical system again and re-calibrate the gain until the requirements
are met.
3. If the gain adjustment of the optical system succeeds, but the CG position of fresh
blood scattergram is still unqualified, check other components of the analyzer
(reaction bath temperature, reagent condition, stirring motor speed).
When the sample scattergram is abnormal and the neutrophil granulocytes in the DIFF
scattergram are divided into two layers, a part of the neutrophil granulocytes are classified as
eosinophilic granulocytes, thus resulting in high Eos# (see below figure), and then the
contamination of flow cell can be confirmed.
Improper transportation, storage, usage and maintenance of the analyzer may cause the
contamination of the flow cell. Dust, oil stains or pollutants on the flow cell deform the laser
beam that passes through the flow cell and rests on blood cells, thus resulting in abnormal
scattergram.
6-27
Optical System
Flow cell contamination can be divided into flow cell exterior wall contamination and flow
cell aperture contamination. The phenomena under the two cases are similar. You need to
observe the optical system to confirm the contamination situation, and then take
corresponding measures.
1. Open the right door of the analyzer when it is on, remove the top panel of the
analyzer and the top cover of the optical system, you can find there are 1-2 reflected
light spots on the front and back faces of the flow cell as shown in below figure.
When the exterior wall of the flow cell is clean, there is no obvious reflected light
spot.
Figure 6-35 The reflected light spots on the exterior wall of the flow cell
2. Put a piece of blank paper or a name card before the high-angle PD assembly (see
below figure), you will find a thick bright optical ring. When the exterior wall of the
flow cell is clean, there is only a weak halo.
The ring
Figure 6-36 The light spot before the high-angle PD when the exterior wall of the flow
cell is contaminated
Resolving method
1. Wipe the surface of the flow cell with clean dust-free cloth and absolute alcohol.
2. When the surface of the flow cell is wiped to the extent described in "Confirmation
method of flow cell exterior wall contamination", that means it is clean;
6-28
Optical System
3. Perform counting for over five times using normal fresh blood and 7um standard
particles under the "OV-WB-CBC+5DIFF" mode; observe the DIFF scattergram to
confirm the cleaning effect.
z Wipe the exterior wall of the flow cell unidirectionally, do not wipe it back ad
forth;
z Do not touch the direct light masking diaphragm when wiping the surface of
the flow cell.
1. Open the right door of the analyzer when it is on, remove the top panel of the
analyzer and the top cover of the optical system, you can find there are 1 reflected
light spot in the center of the flow cell as shown in below figure. When the aperture
of the flow cell is clean, there is no obvious reflected light spot.
Figure 6-37 The reflected light spot in the center of the flow cell when it is
contaminated
2. Put a piece of blank paper before the high-angle PD assembly (see below figure),
you will find a thin sharp optical ring. When the aperture of the flow cell is clean,
there is only a weak halo.
6-29
Optical System
The ring
Figure 6-38 The light spot before the high-angle PD when the aperture of the flow cell
is contaminated
z When clean flow cell runs out of dilution or is filled with bubbles, you can
also see 1 bright reflected light spot in the center of the flow cell. Before
observe the flow cell, please make sure it is filled with dilution and free from
air or bubble.
Resolving method
1. Enter "Service""Maintenance" from the main screen, select "Flow Cell" from
"Probe Cleanser Maintenance" tab, clean the flow cell using probe cleanser
according to the tips displayed on the screen;
2. After finish maintenance, select "Mode""OV-WB-CBC+5DIFF" under the "Count"
screen, run a background counting;
3. Perform counting for over five times using normal fresh blood and 7um standard
particles under the "OV-WB-CBC+5DIFF" mode, observe the DIFF scattergram to
confirm probe cleanser maintenance effect;
4. If the failure persists, perform manual cleaning according to below procedure;
5. Wear clean disposable plastic gloves, connect a long plastic tube to a clean
disposable syringe, and connect a two-way connector to the other end of the tube,
aspirate probe cleanser to soak the tube with the syringe to clean the tubing and the
connector;
6-30
Optical System
Connect
two-way connector
(a) (b)
Figure 6-39 Tube preparation; (a) tube connection preparation; (b) tube cleaning
6. Fill a plastic cup with certain amount of probe cleanser (or use the probe cleanser
reagent bottle directly).Unplug the waste tube of the flow cell from electromagnetic
valve SV6, connect the tube to the cleaned tube of the above step to make the tube
length suitable for operation. Put one end of the tube into the plastic cup filled with
probe cleanser and place the plastic cup on the base plate of the analyzer as shown
in below figure to facilitate service operation;
6-31
Optical System
Connected with
syringe
Figure 6-41 Syringe connection mode when cleaning the flow cell aperture manually
8. Restore tube connection after soaking finishes, run 1 background counting under
"OV-WB-CBC+5DIFF" mode;
9. Perform counting for over five times using normal fresh blood and 7um standard
particles under the "OV-WB-CBC+5DIFF" mode; observe the DIFF scattergram to
confirm probe cleanser cleaning effect.
z Do not plug or unplug fluidic tubing in the optical system, do not put any
fluid (including containers of fluid) into the optical system;
z The syringe can only be pulled instead of being pushed to avoid pollutants
clogging the flow cell channel;
z As the probe cleanser is corrosive to the fluid tubing, do not soak the tubing
for over 10 minutes each time, and the tubing should not be soaked for too
many times.
When the flow cell channel is clogged by clots or foreign substances in the fluidic tubing,
stable sheath fluid cannot be generated, thus resulting into abnormal scattergram. The
phenomena are as follows:
1. The sample scattergram is abnormal, and the DIFF scattergram of normal fresh
blood and 7um standard particle is apparently abnormal as shown in below figure;
6-32
Optical System
Figure 6-42Scattergram of sample and 7um standard particle when the flow cell
aperture is clogged
2. When conduct counting under "CBC-5DIFF" mode, open the right plate of the
analyzer, the dropping speed of waste cistern WC2 is lower than normal speed;
3. Open the top cover of the optical system, no problem is found in the optical system.
Resolving method
1. You can solve this problem by referring to the resolving method of "flow cell aperture
contamination", first use the "Maintenance" function of the analyzer, if the problem
persists, conduct manual cleaning;
2. After finish Step 6 of resolving "flow cell aperture contamination", connect the
sample inlet of the rectifier assembly with a syringe as shown in below figure,
slowly pump the syringe to flush the flow cell channel with probe cleanser and soak
it for 10 minutes.
Connected with
syringe
3. Proceed with Step 7 and the following steps of resolving "flow cell aperture
contamination", connect the syringe with the sheath fluid inlet of the rectifier
assembly and finish cleaning and unclogging.
6-33
Optical System
2. If the spot is not in complete elliptical shape, instead, there are two obvious dark
lines in it, or it is in petal shape, or the brightness of the laser light is low, then the
laser diode is damaged and the optical system needs to be replaced;
3. If the spot shows no obvious abnormity, but is unusually bright, power off the
analyzer, replug the laser power cord and then observe the laser spot. Or check
whether the laser power cord is well-connected or is open-circuited with a multimeter.
If the error is caused by the power cord problem, replace the laser power cord.
z Please make sure that power is off and power cord is disconnected when
removing and installing boards.
z Do not plug or unplug laser semiconductor before power is off, or the laser
semiconductor can be damaged.
2. The DIFF scattergram of normal fresh blood is slightly loose, the border between cell
populations can be observed, the distribution of cell scatters is normal, and DIFF
differential shows no obvious error.
2. After the flow cell is confirmed clean, check whether the light spot on the high-angle
diaphragm is apparently unsymmetrical. See Figure 6-21. If the light spot on the
6-34
Optical System
high-angle diaphragm is apparently unsymmetrical, adjust the optical system per the
method in 6.3.1 .
3. If LAS 0.1 max width is beyond standard, adjust the horizontal position of the direct
light masking diaphragm per the method in 6.3.2 .
4. If MAS 0.1 max width is beyond standard, adjust the horizontal position of the flow
cell per the method in 6.3.2 .
5. After adjustment, run 7um standard particles, make sure the 0.1 max width of
standard particles is qualified.
6. Perform counting for over 5 times using normal fresh blood under the
"OV-WB-CBC+5DIFF" mode, observe the DIFF scattergram to confirm the
adjustment effect.
z The error has nothing to do with the focal length position of the front and
rear light, the diaphragm position and the PD aperture position, please do
not adjust those parts;
6-35
7 Troubleshooting
When error occurs to the analyzer, view recent system log to analyze causes of the error.
CLICK "Service" - "Log", the operations of the analyzer will be displayed. See Figure 7-1.
7-1
Troubleshooting
7-2
Troubleshooting
7-3
Troubleshooting
photocoupler short-circuited
Sheath syringe aspirates DIL floater short or outlet
0x00000138 0x0000310D
too much clogged
Sheath syringe dispenses FCM floater short or outlet
0x00000139 0x0000310E
too much clogged
Sheath syringe works too Cleanser floater short or
0x0000013A 0x0000310F
fast outlet clogged
WC1 cistern dobber
0x0000013B Sheath syringe timeout 0x00003110
open-circuited
WC2 cistern dobber
0x0000013C Pump syringe is working 0x00003111
open-circuited
Pump syringe initialization
0x0000013D 0x00003161 Front cover open
failure
Pump syringe moves to
0x0000013E 0x00003162 Laser box open
start position failure
Pump syringe moves to DIFF stirring motor
0x0000013F 0x00003201
stop position failure abnormal
Pump syringe photocoupler BASO stirring motor
0x00000140 0x00003202
open-circuited abnormal
Pump syringe photocoupler
0x00000141 0x00003211 Laser diode temp. error
short-circuited
Pump syringe aspirates too
0x00000142 0x00003212 Laser diode current error
much
Pump syringe dispenses
0x00000143 0x00003221 Preheat bath temp. error
too much
Pump syringe works too
0x00000144 0x00003222 Reaction bath temp. error
fast
Temperature out of working
0x00000145 Pump syringe timeout 0x00003231
range
Temp. unit: function code Temperature out of
0x00000190 0x00003232
error operating range
Temp. unit: address code
0x00000191 0x00003241 250 KPA pressure abnormal
error
0x00000192 Temp. unit: data length error 0x00007007 Background abnormal
Temp. unit: command ID
0x00000193 0x00007008 Sample not enough
error
Temp. unit: verification code
0x00000194 0x00007009 Sample test sensor error
error
0x00000195 Temp. unit: end code error 0x00007101 Diluent expired
0x00000196 PDB: ADS1210 error 0x00007102 LEO(I) expired
Heater: preheat bath temp.
0x00000197 0x00007103 LEO(II) expired
sensor error
0x00000198 Heater: reaction bath temp. 0x00007104 LBA expired
7-4
Troubleshooting
sensor error
0x00003242 160 KPA pressure abnormal 0x00007105 LH expired
0x00003243 70 KPA pressure abnormal 0x00007106 Cleanser expired
-40 KPA pressure Real-time clock error
0x00003244 0x00007111
abnormal
-85 KPA pressure Network connection error
0x00003245 0xFFFF2000
abnormal
Too many vacancy Network communication
0x00003252 0xFFFF2001
positions error
RBC peak data len not Auto communication
0x00005001 0xFFFF2002
equal to width's operation error
0x00005002 SB: parameter setup error 0xFFFF2003 IP address collision
0x00006001 MB: EEPROM error 0xFFFF3002 Printer out of paper
0x00006101 Open DSPI error 0xFFFF3003 ps data generation error
0x00006102 Read or write DSPI error 0xFFFF3005 Auto-print records full
0x00006103 DSPI data decode error 0xFFFF3006 Printer paper jammed
0x00006111 Open COM(PDB) error 0xFFFF3007 Unknown printer error
WC3 cistern dobber
0x00006112 COM(PDB) ACK timeout 0x00003112
open-circuited
COM(PDB) check sum WC3 cistern is not drained
0x00006113 0x00003113
error
LAS background voltage Diluent volume low
0x00007001 0xFFFFC000
abnormal
RBC aperture voltage LEO(I) lyse volume low
0x00007002 0xFFFFC001
abnormal
0x00007003 HGB error 0xFFFFC002 LEO(II) lyse volume low
0x00007004 HGB adjust 0xFFFFC003 LBA lyse volume low
0x00007005 RBC clog 0xFFFFC004 LH lyse volume low
0x00007006 RBC bubbles 0xFFFFC005 Cleanser volume low
0x0000007C Tube barcode is too long 0xFFFFC006 Insufficient diluent
Mix & pierce unit: Insufficient LEO(I) lyse
0x000000C8 0xFFFFC007
verification code error
Mix & pierce unit: command Insufficient LEO(II) lyse
0x000000C9 0xFFFFC008
ID error
Mix & pierce unit: end code Insufficient LBA lyse
0x000000CA 0xFFFFC009
error
Mix & pierce unit: data Insufficient LH lyse
0x000000CB 0xFFFFC00A
length error
Mix & pierce unit: address Insufficient cleanser
0x000000CC 0xFFFFC00B
code error
Mix & pierce unit: function
0x000000CD
code error
7-5
Troubleshooting
7-6
Troubleshooting
7-7
Troubleshooting
7-8
Troubleshooting
7-9
Troubleshooting
move;
MP: cylinder 3. Pierce photocoupler error. The
0x000000E3 return/photocoupler error phenomenon is pierce cylinder moves;
MP: pierce cylinder pierce is Fluidic sequence or software error, please try
0x000000E4 not allowed again or restart the analyzer.
MP: pierce cylinder return is Fluidic sequence or software error, please try
0x000000E5 not allowed again or restart the analyzer.
Please perform fluidic system initialization
0x000000E9 MP: mix-unit is not initialized and try again.
Pinch cylinder does not move; please check
MP: pinch cylinder to front the pneumatic unit, Valve 89 and the
0x000000EA pos. failed connection wire.
Pinch cylinder does not move; please check
MP: pinch cylinder to rear the pneumatic unit, Valve 89 and the
0x000000EB pos. failed connection wire.
Elevation cylinder does not move, please
MP: elevation cylinder to up check the pneumatic unit, Valve 90 and 91,
0x000000EC pos. failed and the connection wire.
Elevation cylinder does not move, please
MP: elevation cylinder to check the pneumatic unit, Valve 90 and 91,
0x000000ED down pos. failed and the connection wire.
Fluidic sequence or software error, please try
0x000000EF Manipulator is working again or restart the analyzer.
Software error or communication
Syringe unit: function code interference, please try again or restart the
0x0000012C error analyzer.
Software error or communication
Syringe unit: address code interference, please try again or restart the
0x0000012D error analyzer.
Software error or communication
Syringe unit: data length interference, please try again or restart the
0x0000012E error analyzer.
Software error or communication
Syringe unit: command ID interference, please try again or restart the
0x0000012F error analyzer.
Software error or communication
Syringe unit: verification interference, please try again or restart the
0x00000130 code error analyzer.
Software error or communication
interference, please try again or restart the
0x00000131 Syringe unit: end code error analyzer.
Please perform sheath syringe self-test and
0x00000132 Sheath syringe is working try again.
0x00000133 Sheath syringe initialization 1. Sheath syringe photocoupler error The
7-10
Troubleshooting
7-11
Troubleshooting
7-12
Troubleshooting
7-13
Troubleshooting
7-14
Troubleshooting
draining
Normal shutdown F014
Shutdown
Pack-up and shutdown F015
LEO(1) replacement F021
LEO(2) replacement F022
LH replacement F023
LBA replacement F024
Replace reagent
Replace diluent F025
Replace cleanser F026
Fluidic tubing priming of the
F028
analyzer
Cleanser Auto-maintenance (cleanser
F047
maintenance soaking)
RBC bath soaking with probe
F043
cleanser
Premix bath soaking with probe
F044
cleanser
HGB bath soaking with probe
F045
cleanser
SRV maintenance with probe
F059
cleanser
WBC module maintenance with
F060
probe cleanser
Analyzer maintenance with
F061
probe cleanser
Flow cell maintenance with
F062
probe cleanser
Flushing F048
Aperture unclogging Zapping F049
Unclogging F050
Flow Cell Bubble Removal F051
RBC bath cleaning F052
Pre-mix bath cleaning F053
Components
WBC bath cleaning F054
cleaning
HGB bath cleaning F055
Open Vial unit cleaning F056
Piercing unit cleaning F057
Draining/priming Fluidic system cleaning F058
DIL bath priming F071
FCM bath priming F072
Cleanser cistern priming F073
WC1 cistern draning F081
WC2 cistern draning F082
7-15
Troubleshooting
During the operation, if an error is detected, the analyzer will display the corresponding error
message on the upper left corner of the screen. In the error message area, the severity levels
are indicated by background colors, with red for the highest and blue for the lowest.
CLICK the error message on the screen and the corresponding help information will pop up.
CLICK the "Remove Error" button as instructed by the help information to remove errors.
Some errors can also be removed as instructed by the manual.
7-16
Troubleshooting
7-17
Troubleshooting
No Diluent Diluent has run out or Diluent in the diluent container has run
the sensor connected out.
with the diluent tube is in The sensor of diluent tube is not well
poor contact. connected or is damaged; the sensor
needs to be replaced.
No LH lyse The LH lyse has run out Lyse in LH lyse container has run out.
or the sensor connected The sensor of LH lyse tube is not well
with the LH lyse tube is connected or is damaged; the sensor
in poor contact needs to be replaced.
No LEO(I) lyse The LEO(I) lyse has run Lyse in LEO(I) lyse container has run out.
out or the sensor The sensor of LEO(I) lyse tube is not well
connected with the connected or is damaged, the sensor
LEO(I) lyse tube is in needs to be replaced.
poor contact
No LEO(II) lyse The LEO(II lyse has run Lyse in LEO(II) lyse container has run
out or the sensor out.
connected with the The sensor of LEO(II) lyse tube is not
LEO(II) lyse tube is in well connected or is damaged, the sensor
poor contact needs to be replaced.
No LBA lyse The LBA lyse has run Lyse in LBA lyse container has run out.
out or the sensor The sensor of LBA lyse tube is not well
connected with the LBA connected or is damaged, the sensor
lyse tube is in poor needs to be replaced.
contact
No Cleanser Cleanser has run out or Diluent in the cleanser container has run
the sensor connected out.
with the cleanser tube is The sensor of cleanser tube is not well
in poor contact. connected or is damaged, the sensor
needs to be replaced.
Waste cistern is full The waste cistern is full Diluent in the waste cistern has run out.
or the sensor connected The sensor of waste tube is not well
with the waste cistern is connected or is damaged, the sensor
in poor contact. needs to be replaced.
Diluent has expired Diluent expired
Diluent expired The expiration setting of diluent is
incorrect.
LEO(I) expired LEO(I) lyse has expired LEO(I) lyse has expired.
7-18
Troubleshooting
1. Remove the side plate, top plate, main board, and the protective cover of signal
board, and take off the protective cover of volumetric board;
2. Remove the four screws fixing the volumetric board, take out the board without
moving other components. Pay attention to electrostatic prevention. Remove the
glue on VR3 and VR4 to prepare for adjustment;
3. Take off the signal wires of volumetric board and reconnect the brown wire to the
signal board and volumetric board, the 2 indicators on the volumetric board will light
up;
7-19
Troubleshooting
4. After the indicators turn on, set multimeter to measure DC voltage, connect the black
pen-shape detector of the multimeter to TPGND, the red pen-shape detector
monitors the voltage of TP2 and TP3. Adjust potentiometer VR4 and VR3 with
horologic driver to turn the voltage of each test point to 2.55-2.65V, if fails, please
replace the volumetric board; then put volumetric tube into the volumetric board, test
the voltage of VR3 and VR4, which should be 2.90V; if it cannot reach 2.9V, replace
the volumetric tube.
5. Lift the latch plate of the connector lightly with nipper and connect the brown wire to
the connector. Make sure that the connector is fixed securely.
7-20
Troubleshooting
3. Adjust VR1 with the driver (pay attention to the difference between VR1 and VR2),
observe whether the measured values change, and adjust voltage of the test point to
3.50.1V;
4. Block the sending and receiving ends of the sensor with non-transparent object, then
the output voltage should be 00.05V; otherwise the sensor is damaged.
5. Finally press the TELFON tube into the sensor slot, the output voltage should be no
less than 1.5V; otherwise the sensor is damaged.
7-21
Troubleshooting
7-22
Troubleshooting
Monocytes differential
Inaccurate volume of 4-DIFF differential
result is too high.
reagents is added in. LEO(I) should be
Results of most normal
1.02ml and LEO(II) should be 0.32ml.
samples are 10%
Monocytes too high Polluted DIFF reaction bath.
higher. The division
Reaction bath temperature is out of the
between monocytes and
normal range. The reaction is not
neutrophils is not
sufficient.
distinct.
WBC analysis results: If cells are differentiated in the DIFF
Spots in DIFF Spots in DIFF, and scattergram, but the ghost cells and
No spot in BASO mostly there are many lymphocytes stick together, the RBC
ghost cells; little or even hemolysis is not sufficient. The reason
7-23
Troubleshooting
7-24
Troubleshooting
7-25
Figure 7-5 Analyzing process of low WBC results
7-26
Troubleshooting
z The unit of RBC count time is 0.1s and the unit of RBC baseline is ms.
When the sum of the RBC baseline time that is over the upper and lower limits is greater than
50 of RBC count time, there are impurities in the sample or liquid, please check;
7-27
Troubleshooting
7-28
Troubleshooting
Reagent expired
or contaminated?
Gain setup
(2.28V)
HGB indicator on?
Connection OK?
Indicator unit abnormal?
HGB indicator
normal? The wall of HGB
bath dirty?
7-29
Troubleshooting
When conducting
background count, the
difference value between
HGB blank voltage and
measurement voltage is
less than 3.
RBC count time must be in
the range of set value
0.25s, the START delay
time must be in the range of
set value 0.5s.
7-30
Troubleshooting
z If no problem is found with the circuits and main board, the error may lie
with the inverter. However, as there is no valid test point between the
inverter and the display, the error can only be verified by replacing the
inverter and the display.
Internal tube of
Vacuum and
Pneumatic Connection pneumatic unit
Pneumatic unit pressure are -85k
unit on? correct? and 250k
is loose?
Air pump broken
Pressure
Pressure Inlet status of the Replace the
adjusted
regulator regulator correct? regulator
correctly?
Replace
Pressure Replace with other pressure
sensor pressure sensor? detection
Normal? board
Unplug the power cords of the main board and signal processing board, the power supply
board only provides power to the power driver board, restart the analyzer to see if the error still
exists. If the indicator on the front plate turns green, the error has been removed, otherwise the
error persists. If the error disappears, then the short-circuit of 5V power is caused by other
boards, please go on with Step ; otherwise, the driver board or its peripheral wires or
components are short-circuited, please go on with Step 2.
The power supply board only provides power to the driver board and signal processing
board, restart the analyzer to see if the error still exists. If yes, the error is caused by the signal
7-31
Troubleshooting
processing board and its peripheral wires; if no, the error is caused by the main board.
2. Make sure short-circuit occurs inside or outside the board. If it occurs inside the board,
the board needs to be replaced; if it occurs outside the board, check which wire
causes the error.
Unplug all wires of the board that have 5V power signal (excluding the power cord), restart
the analyzer to see if the error is removed. If yes, the error is caused by external wires or
components, go on with below Step ; if not, the error lies with the board itself, remove
impurities (dust or water stain) on the board, restart the analyzer, if the error still exists, replace
the board.
Add a piece of wire each time to connect with the board, if the error occurs, then the
short-circuit of 5V power is caused by the wire added, go on with Step 3; if the error disappears,
turn off the power and connect another piece of wire, and then turn on the power; repeat the
process until the error occurs or all wires are checked.
3. Make sure which component or the wire itself is short-circuited, find out the reason
and eliminate the error.
z Pay more attention to the photocoupler and connection wire of the driver
board.
7-32
Troubleshooting
z Pay more attention to the WBC bath and its connection wires to see if the
erosion of them causes the short-circuit of 24V power.
Figure 7-12 Abnormal scattergram - spots scatter all over the scattergram
Troubleshooting
1. Please check whether the diluent container is empty. Sometime the floater does not
report error when the container gets empty;
2. Open the right door, check whether there are diluent in the FCM cistern; if no, prime
the FCM cistern through "Maintenance" screen;
3. If after performing "Prime FCM Cistern", no fluid enters into the cistern, check if the
SV53 is normal. If the counting process cannot start and stop normally, then the valve
must be damaged.
4. If Burkert valve SV53 can open and close normally, then the air valve controlling
pressure and vacuum switching of the FCM cistern must be damaged, GV80 needs to
be replaced.
If vertical lines appear on both sides of the scattergram (see Figure 7-13), that is because air
enters into reagent tubing. Check whether reagent bottles were replaced recently or the
reagent tubing is loose. Then perform background counting for several times, the error can be
eliminated.
7-33
Troubleshooting
Figure 7-14 7um standard particle scattergram (abnormal - flow cell contaminated)
Troubleshooting
Refer to Chapter 6 6.4.1 Sample scattergram is abnormal for troubleshooting.
The direct light masking diaphragm of the rear light unit is not
symmetrical
Error phenomenon:
WBC differential effect becomes poor, neutrophils decrease and lymphocytes increase. After
perform counting using 7um standard particles under OV-WB mode, the spots are not
well-clustered, low angle DIFF scattergram is dispersed. See below Figure 7-15:
7-34
Troubleshooting
7-35
Troubleshooting
z All parameters should not be less than 3 except for "RBC lyse resist".
When the flag sensitivity parameters are within the range 3-8, the flag frequency should be
acceptable. If the flag frequency of a parameter is exceptionally high, and the sample is normal,
then the analyzer itself needs to be checked.
The flag sensitivity parameters can be set as follows:
Login the "Main" screen with the service engineer access level and CLICK the
"Setup" icon to enter into the "Setup" screen.
After entering the "Setup" screen, CLICK the "Advanced" button to enter the
"Advanced" screen.
In the "Advanced" screen, CLICK the "Edit" button under the "Flag Sensitivity", the
edit box of parameter sensitivity pops up (Figure 7-17). Then adjust the setup
parameters of the corresponding flags according to the users requirements. After the
setup is finished, CLICK Ok and quit the "Advanced" screen.
7-36
Troubleshooting
Login the "Main" screen with the service engineer access level and CLICK the
"Setup" icon to enter into the "Setup" screen.
After entering the "Setup" screen, CLICK the "Advanced" button to enter the
"Advanced" screen.
In the "Advanced" screen, CLICK the pull-down button under the "Non-diff
sensitivity" and the pull-down list of the "Non-diff sensitivity" pops up (Figure 7-18).
Then adjust the setup parameters of non-diff algorithm according to the advice
provided by "R&D". After the setup is finished, quit the "Advanced " screen and save
the parameters.
7-37
8 Maintaining Your Analyzer
8-1
Maintaining Your Analyzer
8-2
Maintaining Your Analyzer
8-3
Maintaining Your Analyzer
1. Turn off the pneumatic unit, put a piece of dry cloth under SRV to clean the leftover
reagent or liquid when removing it from the analyzer.
2. Pull out the leftover liquid tray under the SRV, clean it with clear water.
3. Move the bracket of the open vial probe wipe down until the probe wipe gets out of the
sample probe.
4. Turn the hand hold of the spring counter-clockwise to remove it, take off the sampling
valve and rotor valve of SRV. See Figure 8-4.
8-4
Maintaining Your Analyzer
5. Aspirate probe cleanser with a syringe and dispense it into the holes and slots of the
sampling valve, rotor valve and rear fixed valve, and clean them lightly. See Figure 8-5.
6. Wipe the contacting faces between the three valves with clean wet cloth and probe
cleanser, and then wash them with distilled water.
8-5
Maintaining Your Analyzer
z The hand hold of rotor valve should face to the analyzer; the curved surface
of rotor valve should face up. After installation turn the hand hold of rotor
valve to up position.
8.2.5 Cleaning Open Vial Probe Wipe and Pierce Probe Wipe
1. Pull the bracket of the probe wipe down to the utmost, and remove the open vial probe
wipe lightly from the sample probe;
2. Remove the open vial probe wipe from the bracket, and pull off the pipe connected
with the probe wipe. See Figure 8-6:
3. Wash the open vial probe wipe with clear water, then dry it and install per the removing
procedure in reverse order.
4. Clean pierce probe wipe and the exterior wall of pierce probe with clean cloth and
clear water. See Figure 8-7
8-6
Maintaining Your Analyzer
Open the optical shielding box, wrap a cotton stick with lens wiping paper (generally it can be
found in all hospitals), wipe the exterior wall of the flow cell from center to sides to make it
clean.
8-7
Maintaining Your Analyzer
Enter "Service" "Init." "Runs init.", CLICK on specific information. See Figure
8-9.
Confirm piercing times of the pierce probe; if it is over 36,000, please replace the
pierce probe unit; if not, clean the probe wipe.
Check whether the two yellow pinch tubes at the outlet of WBC bath have been used
for over 12 months. If yes, replace them.
Check whether there is liquid in the isolation chamber on the back panel that is
8-8
Maintaining Your Analyzer
connected with the vacuum. If yes remove the isolation chamber and pour out the
liquid.
Replace the filter core once every two years. Refer to for the
operation.
2. Enter the main screen, CLICK "Service" "Clean" "Overall" to perform overall
maintenance.
3. Perform cleaning for once or background count for 3 times, if still not qualified, check
the cleaning procedure above and perform cleaning again (till now, overall
maintenance is finished).
2 Verify the accuracy of the analyzer by performing counting with calibrators and controls. If
still not qualified, re-perform calibration.
3 Perform open vial and autoloading test using fresh blood (more than 10 tubes). The precision
and repeatability must be high and scattergram must be the same as shown in Figure 8-10:
8-9
9 Appendices
A-1
Appendices
A-2
Appendices
A.3 Consumable
No. Description MINDRAY Package
code/model Specification
94. Tubing. PU tube 4mmX2.5mm transparent M6G-020002--- 20M
95. Tubing. PU tube 6mmX4mm transparent M6G-020003--- 20M
96. Tubing. NB tube 6mmX4mm white M6G-020004--- 20M
97. Tubing. MPF tube 2mmX3.5mm M6G-020005--- 50FT
98. Tubing. Silicone, 1/16"X3/16", TYGON 3350 M6G-020006--- 50FT
99. Tubing. Chemfluor FEP, 0.062"ODX0.031"ID M6G-020008--- 50FT
100. Tubing. Silica gel 0.031"ID 0.156"OD 50ft/ coil M6G-020009--- 50FT
101. Tubing. PharMed, 1/16"ODX1/8"ID M6G-020011--- 25FT
102. Tubing. PU,ID: 3/32", OD3/16", clear M6G-020054--- 100FT
103. Tubing. 3/32"X5/32", S-50-HL AAX02004,
M90-100071--- 100FT
Tygon
A-3
Appendices
A-4
Appendices
A.5 Cable
No. Material Description MINDRAY code/model
1. USB extension line 009-000135-00
2. Power cord harness 009-000200-00
3. Connecting line connecting main board and driver board 3100-20-41288
USB connecting line connecting main board and
4. 3100-20-41289
interface board
Network connecting line connecting main board and
5. 3100-20-41290
interface board
6. TFT display signal line 3100-20-41291
7. Hard disk signal line 3100-20-41292
8. Touchscreen signal line 3100-20-41294
9. Power cord of TFT screen converter 3100-20-41295
10. Hard disk power cord 3100-20-41296
11. Electromagnet control line 3100-20-41297
12. LAS signal line 3100-20-41298
13. MAS signal line 3100-20-41299
14. RBC/PLT signal line 3100-20-41300
15. Volumetric signal line 3100-20-41302
16. HGB signal line 3100-20-41303
17. Fan and connecting line of the analyzer 3100-20-41304
18. Screen ground wire 3100-20-41305
19. Control line of laser heater 3100-20-41306
20. Control line of valve 1 to 16 009-000176-00
21. Control line of valve 17 to 32 009-000177-00
22. Control line of valve 33 to 48 3100-20-41309
23. Pump transferring line 3100-20-41310
24. Connecting line harness of tube rack transport unit 3100-20-49003
25. Control line of Y direction transport motor 3100-20-49003-01
26. Control line of X direction transport motor 3100-20-49003-02
Position sensor transferring line of tube rack transport
27. 3100-20-49003-03
unit
28. Connecting line of pneumatic compensating capacitor 3100-20-49004
29. Connecting line of pneumatic unit temperature switch 3100-20-49005
30. Connecting line harness of piercing&mixing unit 009-000181-00
31. Connecting line harness of motors and sensors 009-000184-00
32. Control line of whole blood aspirating syringe motor 3100-20-49007-01
33. Pump connecing line 3100-20-49007-02
34. DC motor control line 3100-20-49007-03
35. Control line of WBC sample dispensing syringe motor 3100-20-49007-04
36. Connecting line of syringe unit position sensor 009-000185-00
A-5
Appendices
A-6
Appendices
A-7
Appendices
B-1
C Fluidic Diagram
C-2
Appendices
SV1 Work with GV52, SV07 and SV08 to clean wBC sample preparing tubing
Work with SV06 to drive sample; work with SV07 and SV08 to clean sample
SV2
dispensing syringe tubing
Work with SV7 and SV8 to back flush sample probe; work with SV6 to form
SV3
sheath fluid path
SV4 Not available
Work with DP8 to dispense diluent and sample in SRV (altogether 2.6ml) to
SV5 RBC premix bath, or to dispense 2.6ml diluent and first dilution sample in SRV
to RBC bath
SV6 Flow cell waste outlet
SV7 Work with DP4 to send sample in WBC bath to sample preparing tubing
SV8 Waste outlet of sample preparing tubing
SV9 Control the fluid flow direction of whole blood aspirating syringe
Work with whole blood aspirating syringe SV09 to drive sample into SRV;
SV10
clean SRV and sample probe;
Work with M50 cleanser cistern and SV13 to dispense cleanser into
SV11
WBC-realted channels
Work with M50 cleanser cistern and SV13 to dispense cleanser into
SV12
HGB-realted channels
SV13 Control priming of M50 cleanser cistern to all channels
Work with M50 cleanser cistern and SV13 to dispense cleanser into
SV14
RBC-realted channels
Work with GV78 to prime reagent in M-50 cleanser container to M-50 cleanser
SV15
cistern under the effect of vacuum
Work with V13 and V10 to dispense cleanser into sampling channel; work with
SV16 DP4, SV7, GV52, SV11 and SV10 to aspirate probe cleanser outside the
analyzer
SV17 Work with DIL cistern, GV50 and GV51 to dispense diluent into WBC bath;
SV18 Wash SRV and DIFF blood-dispensing channel under CBC mode
SV19 Work with DP7 to dispense 2ml of diluent into HGB bath
SV20 Work with GV55 to wash the exterior wall of pierce probe
SV21 Work with GV56 to wash the exterior wall of open vial sample probe
SV22 Work with DP8 and SV5 to dispense 2.6ml of diluent into RBC bath or RBC
D-1
Appendices
premix bath
SV23 Work with DP6 to aspirate first dilution sample from RBC premix bath
SV24 Work with GV57 to clean SRV and its surrounded area
SV25 Work with DP1 to add 0.32ml of LEO II lyse into DIFF bath
SV26 Work with DP2 to add 0.52ml of LEO I lyse into DIFF bath
SV27 Work with DP3 to add 0.52ml of LBA lyse into BASO bath
SV28 Work with DP5 to add 1ml of LH lyse into HGB bath
SV29 Waste draining of RBC premix bath
SV30 Waste draining of BASO bath
SV31 Waste draining of DIFF bath
SV32 Water draining of pressure filter
SV33 Work with SV38 to dry RBC volumetric tube
SV34 Wash the back bath of RBC bath
SV35 Draining HGB bath
SV36 Draining RBC bath
Connected with RBC back bath and volumetric tube, aspirate fluid of RBC front
SV37
bath to back bath under the effect of WC3 vacuum
SV38 Work with SV33 to dry RBC volumetric tube
D-2
Appendices
Control PV3, work with SV24 to drain the waste after cleaning the surrounded
GV57
area of SRV to WC1 under the effect of vacuum
Control PV2, work with GV77 to drain the waste in WC1 to outside waste
GV58
container under the effect of pressure
GV59 Control PV1, drain waste in WC2 to WC1 under the effect of vacuum
Control SRV moving cylinder, switch SRV to the blood dispensing status of
GV60
open vial mode
Control SRV moving cylinder, switch SRV to the sampling status of open vial
GV61
mode
GV65 Pump bubbles into HGB bath
GV66 Pump bubbles into RBC premix bath
GV67 Pump bubbles into RBC bath
Work with GV55 and waste pump to dry the air discharging channel of
GV68
autoloading sample probe under the effect of pressure
Control DP1, work with SV25 to dispense 0.32ml of LEO(II) reagent into DIFF
GV69
bath
GV70 Control DP5, work with SV28 to dispense 1ml of HGB lyse into HGB bath
Control DP2, work with SV26 to dispense LEO(I) reagent and sample in SRV
GV71
(altogether 0.52ml) into DIFF bath
Control DP3, work with SV27 to dispense LBA reagent and sample in SRV
GV72
(altogether 0.5ml) into BASO bath
Control DP7, work with SV19 to dispense diluent and sample in SRV
GV73
(altogether 2ml) into HGB bath
Control DP8, work with SV22 to dispense diluent and sample in SRV
GV74 (altogether 2.6ml) into RBC premix bath; work with SV22 and SV5 to dispense
diluent and first dilution sample in SRV (altogether 2.6ml) into RBC bath
Control DP4, work with SV7 and GV52, aspirate sample in DIFF/BASO bath to
GV75
sample preparing tubing under the effect of vacuum
Control DP6, work with SV23, aspirate first dilution sample in premix bath into
GV76
sample back-aspirating tubing
Work with GV58 to drain the waste in WC1 to outside waste container under
GV77
the effect of pressure
Work with SV15 to prime cleanser into cleanser cistern under the effect of
GV78
vacuum
GV79 Work with GV54 to prime diluent into DIL cistern under the effect of vacuum
GV80 Work with GV53 to prime diluent into FCM cistern under the effect of vacuum
Control the forward and backward moving cylinder of mixing unit; the
GV89
manipulator moves forward and backward
Control the up and down moving cylinder of mixing unit; when it is on, the
GV90
manipulator moves down
GV91 Control the up and down moving cylinder of mixing unit; when it is on, the
D-3
Appendices
manipulator moves up
PV1 (8mm) Controlled by GV59, discharge waste in WC2 cistern into WC1 cistern
PV2(8mm) Controlled by GV58, discharge waste in WC1 cistern into waste container
Controlled by GV57, discharge the waste after cleaning the surrounded area of
PV3 (8mm)
SRV into WC1 cistern
Controlled by GV56, discharge the waste after cleaning open vial probe wipe
PV4 (8mm)
into waste pump
Controlled by GV55, discharge the waste after cleaning autoloading probe
PV5 (8mm)
wipe into waste pump
PV6(8mm) Controlled by GV49, discharge waste in WC3 cistern into WC1 cistern
PV10 (5mm) Controlled by GV52, clean and control the lower tubing of WBC unit
PV11 (5mm) Controlled by GV51, control the flow direction of fluid in DIFF bath
D-4
Appendices
PV12(5mm) Controlled by GV50, control the flow direction of fluid in BASO bath
Description Function
D-5
Appendices
E Tubing
Tube naming principle: connector No. - tube model - tube number - tube length - connector
No.
For example: 73-4-T167-205-J47, J73 and J74 are connector numbers, 4 is tube model,
T167 is the tube number in 3110, 205 stands for tube length (the unit is mm).
E-1
Appendices
E-2
Appendices
19-T200-20
19-T199-20
19-T120-50
19-T203-35
C22 C23
DP7
DP8
DP1 DP2
DP3
DP5 DP6 DP4
E-3
Appendices
C19 19-T135-100
DP7
DP8
DP1 DP2
DP3
DP5 DP6 DP4
E-4
Appendices
15-T236-15
15-T233-20
GF1
15-T237-15
15-T303-120
15-T313-50
15-T343-35
C96
GV92
C86 15-T230-15
15-T344-20 C108 GV89
GV90
15-T345-30
GV68
GV80 GV74 C124 GV91
15-T349-35
15-T308-180
J101-21-T312-100-J102 GV60
15-T346-20 GV61
C109
GV73 C125
GV79 15-T314-15
15-T315-30
GV67
15-T347-60
GV49
GV78 GV72 C126
15-T316-15 GV50
15-T318-30
GV51
GV52
GV77 GV71 C127
15-T238-250
GV66 15-T317-15 15-T320-30
GV58
15-T310-60
GV59
15-T260-15
15-T251-15
GV65 15-T321-15
GV75
GV69
C129
15-T239-15
GF2
C90 C91
E-5
Appendices
15-T271-1800
10-T269-25 10-T270-25
15-T272-35
C97 15-T284-120
GV92 15-T261-50
C119
C111 15-T286-20 GV89
12-T257-200 C104 15-T273-20
GV68 GV90
12-T262-40
GV80
15-T274-40
GV74 GV91
C120
GV60
15-T288-20
GV61
C114
15-T265-20 J99-21-T279-600-J100
C112 GV73
GV79 C105 C121
15-T263-30
15-T275-20
15-T291-30
GV67 15-T290-20
15-T266-20
15-T276-50
GV49
GV78 C106 GV72
C122 GV50
15-T280-125
15-T293-30
GV51
15-T292-20
C113 GV52
15-T264-40
GV77 15-T277-20 GV71
GV66
C123
15-T294-20 GV55
15-T278-80
C116 GV56
GV76 15-T281-20 GV70
GV57
15-T282-30
GV58
15-T295-60
GV59
15-T283-20 GV65
GV75
GV69
C117
GF2
E-6
Appendices
DIL
FCM
Large cable
clip 6-T23-790
6-T22-880
OUT OUT
SV53 SV54
NC NC
6-T20-50
6-T19-50 6-T18-190 M-58D
PV6 C45
WC3 5-T70-300 5-T187-50
bottom
5-T218- PV5 C62 5-T189- WC
Closed tube 340 1
probe wipe 1100 C64
Open vial 5-T100- PV4
probe wipe 1290 5-T188-50
15-T348-
PV3 C63 5-T193-
260
SRV sampling 4-T74-150- 100
5-T75-530 WC1
valve M J58
C51 PV2 bottom
5-T192-420
E-7
Appendices
E-8
Appendices
E-9
Appendices
E-10
Appendices
E-11
Appendices
E.12 Connector
Name Part No. Description Quantity
C14, C16, C17, C20, C21, C25, C26,
C27, C30, C31, C36, C37, C38, C42,
C44, C46, C47, C51, C53, C54, C55, I connector -
C61, C98, C99, C100, C102, C132 M90-100027--- large - medium 27
I connector -
C4, C18, C83, C101, C104, C130 M90-100028-01 medium 6
L connector -
thread - quick
C67, C68, C72, C73, C74, C75, C93 M6Q-030006--- plug 9
C1, C56, C58, C87, C89, C90, C91, L connector -
C117, C118, C129, C131 M90-100100--- medium 11
C24, C60, C65 M90-100065--- T connector 3
C2, C3, C5, C6, C7, C8, C9, C10, C11,
C12, C13, C15, C19, C22, C23, C28,
C29, C33, C34, C35, C43, C49, C50,
C52, C67, C86, C88, C96, C97, C103,
C105, C106, C110, C116, C124, C125, T connector -
C126, C127, C128 M90-100028--- medium 39
Y connector -
C45, C62, C63, C64 M90-100030--- large 4
C32, C39, C40, C41, C48, C66, C108,
C109, C111, C112, C113, C114, C115, Y connector -
C119, C120, C121, C122, C123 M90-100028-03 medium 18
Y three-way
C76, C78, C92 M6Q-030003--- connector - large 3
Y three-way
connector - large
C77, C94, C133, C134 M6Q-030002--- - medium 4
Y three-way
connector -
thread - quick
C95 M6Q-030004--- plug 1
L connector -
thread - quick
C79, C80, C81, C82 M6Q-030005--- plug(R1/4) 4
Volumetric tube
C59 3001-10-07066 connector 1
E-12
Appendices
Threading
chaser tube -
thread - quick
C84, C85 M6Q-030001--- plug 2
E-13
Appendices
E-14
Appendices
E-15
Appendices
Valves and
T30 4-45 pumps Thick TELFON tube 45
Valves and
T32 4-45 pumps Thick TELFON tube 45
Valves and
T34 4-45 pumps Thick TELFON tube 45
E-16
Appendices
Valves and
T44 8-50 pumps TPU tube 50
Valves and
T45 8-20 pumps TPU tube 20
Valves and
T46 8-20 pumps TPU tube 20
Valves and
T47 19-15 pumps 50 thin tube 15
E-17
Appendices
Valves and
T71 8-100 pumps TPU tube 100
E-18
Appendices
Valves and
T72 8-15 pumps TPU tube 15
Valves and
T76 8-40 pumps TPU tube 40
Valves and
T77 8-20 pumps TPU tube 20
Valves and
T78 8-130 pumps TPU tube 130
Valves and
T81 8-15 pumps TPU tube 15
Valves and
T82 8-15 pumps TPU tube 15
Valves and
T83 19-90 pumps 50 thin tube 90
Valves and
T84 8-130 pumps TPU tube 130
Valves and
T85 19-25 pumps 50 thin tube 25
E-19
Appendices
Valves and
T96 8-40 pumps TPU tube 40
Valves and
T97 8-15 pumps TPU tube 15
E-20
Appendices
E-21
Appendices
Valves and
T120 19-50 pumps 50 thin tube 50
Valves and
T121 8-35 pumps TPU tube 35
Valves and
T122 8-15 pumps TPU tube 15
Valves and
T131 8-25 pumps TPU tube 25
E-22
Appendices
Valves and
T132 8-15 pumps TPU tube 15
Valves and
T133 8-100 pumps TPU tube 100
Valves and
T134 8-130 pumps TPU tube 130
Valves and
T135 19-100 pumps 50 thin tube 100
Valves and
T140 4-55 pumps Thick TELFON tube 55
Valves and
T141 8-100 pumps TPU tube 100
Valves and
T142 8-180 pumps TPU tube 180
Valves and
T143 19-60 pumps 50 thin tube 60
Valves and
T144 19-20 pumps 50 thin tube 20
Valves and
T145 4-220 pumps Thick TELFON tube 220
E-23
Appendices
Valves and
T149 8-90 pumps TPU tube 90
Valves and
T150 8-15 pumps TPU tube 15
Valves and
T152 19-20 pumps 50 thin tube 20
Valves and
T153 4-65 pumps Thick TELFON tube 65
Valves and
T154 19-47 pumps 50 thin tube 47
Valves and
T155 19-34 pumps 50 thin tube 34
Valves and
T156 19-17 pumps 50 thin tube 17
Valves and
T157 19-180 pumps 50 thin tube 180
Valves and
T158 19-17 pumps 50 thin tube 17
E-24
Appendices
Valves and
T161 19-34 pumps 50 thin tube 34
Valves and
T162 19-17 pumps 50 thin tube 17
Valves and
T163 19-20 pumps 50 thin tube 20
Valves and
T164 19-30 pumps 50 thin tube 30
Valves and
T165 1-1000 pumps Thick soft tube 1000
Valves and
T166 19-30 pumps 50 thin tube 30
Valves and
T167 4-205 pumps Thick TELFON tube 205
E-25
Appendices
Valves and
T182 19-60 pumps 50 thin tube 60
Valves and
T183 19-230 pumps 50 thin tube 230
Valves and
T184 19-30 pumps 50 thin tube 30
E-26
Appendices
Valves and
T199 19-20 pumps 50 thin tube 20
Valves and
T200 19-20 pumps 50 thin tube 20
Valves and
T203 19-35 pumps 50 thin tube 35
Valves and
T204 19-80 pumps 50 thin tube 80
E-27
Appendices
E-28
Appendices
E-29
Appendices
E-30
Appendices
E-31
Appendices
E-32
Appendices
E-33
Appendices
E-34
Appendices
E-35
Appendices
E-36
Appendices
E-37
Appendices
E-38