From Eye to Insight

Leica Application Suite

Leica Application
Suite
System Requirements V4.8.0

Release Notes V4.1.2

Leica Application Suite
System Requirements
Includes computer specifications for other Leica Industry Division Software

Abstract
This document describes the microscopes, cameras and accessories that are supported
by this version of LAS and defines the computer system requirements. Read this
document before installing your copy of this software.

All reasonable steps have been taken to ensure that this publication is correct and
complete, but should any user be in doubt about any detail, clarification may be sought
from Leica Microsystems (Switzerland) Ltd, or their accredited representative. The
information in this document is subject to change without notice and should not be
construed as a commitment by Leica Microsystems (Switzerland) Ltd. Leica
Microsystems (Switzerland) Ltd accepts no responsibility for any errors that may
appear in this document.

Copyright 2015 Leica Microsystems (Switzerland) Limited.

All rights reserved. The contents of this publication may not be reproduced in any
form, or communicated to a third party without prior written permission of Leica
Microsystems (Switzerland) Ltd.

Due to a policy of continuous development, we reserve the right to change

specifications without notice.

Microsoft and MS-DOS are registered trademarks and Windows, the Windows logo, the
Windows XP logo, the Windows 7 logo and the Windows 8 and 8.1 logo are trademarks
of Microsoft Corporation.

Date: Oct 2015

Issue: 31
Table of Contents
1 Supported Microscopes, Cameras and Accessories 1
Leica DM Microscopes ........................................................................................1
Leica DM Accessories .........................................................................................1
Leica Stereo- and Macroscopes ..........................................................................1
Leica Digital Microscope Cameras ..................................................................... 4
2 Computer System (PC) Specification 5
Leica Software Products using this Specification .............................................. 5
Computer Hardware Requirements ................................................................... 6
Notebook Computers ......................................................................................... 8
3 Power Mosaic and MultiStep Components 9
4 Factors that may reduce performance 12
 1 Supported Microscopes, Cameras and Accessories
The following Leica microscopes and digital cameras are supported.

For other Leica Industry division software products please refer to the
software product specifications for supported hardware and not this section.

For LAS Power Mosaic, please see Section 3 for supported equipment.

Leica DM Microscopes
DM8000, DM12000

DM1000, 2000, 2500, 2700 M/P, 3000, 4000 B/M, 4500 B, 4500 P, 5000
B, 5500 B, 6000 B/M
DM4, 6
DMI8, 3000, 4000 B, 5000 M and 6000 B

DM500, DM750, DM750 P, DM1750, DM IL

DM2000 LED / DM3000 LED

DM4000 B LED, DM4000 M LED and DM4500 P LED

FSC, FSM

CTR 4000-6500, 7000

Leica DM Accessories
Scanning stages

External light source EL6000

External filter wheels (configure only, not used by LAS)

TIRF module (configure only, not used by LAS)

Motorized duo documentation tube

Oasis-blue XYZ controller

11501261 Motorized stage 75x50 with integrated controller for DM4000-

6000 B (not Power Mosaic)

Leica Stereo- and Macroscopes

M205 A, fully motorized stereomicroscope with 20.5:1 zoom range

M205 FA, fully motorized fluorescence stereomicroscope with 20.5:1 zoom

range

M205 C, coded stereomicroscope with 20.5:1 zoom range

LAS System Requirements Page 1

 M165 FC, manual fluorescence stereomicroscope with coded filter wheel, zoom
and iris
and 16.5:1 zoom range

M165 C, coded stereomicroscope with 16.5:1 zoom range

M125, manual stereomicroscope with 12.5:1 zoom range

Zooms in configuration DVM2000

VZ85, VZ85 R, VZ85 C, VZ85 RC, VZ105, VZ105 C, VZ15 M

Zooms in configuration DVM2500

VZ10, VZ10 M, VZ10 MC, VZ18 BGA, VZ75, VZ75 C, VZ80, VZ80 R, VZ80 C, VZ80
RC, VZ100, VZ100 C, VZ700 C

DVM3000 and DVM5000 (for firmware update only)

LED illumination LED3000 RL (Ring Light), LED3000 SLI (Spot Light

Illumination), LED3000 NVI (Near Vertical Illumination)

LED illumination LED5000 RL (Ring Light), LED5000 SLI (Spot Light

Illumination), LED5000 MCI (Multi Contrast Illumination), LED5000 CXI
(Coaxial Illumination), LED5000 HDI (High Diffuse Illumination)

LED illumination for VZ series (10 450 433 and 10 450 432)

M-Series Long / Short Focus Columns (10 450 257 and 10 450 022)

M-Series Long / Short Motor Focus Columns (10 450 258,10 450 050, 10 450
502, 10 450 503))

USB interface for new manual focus drive coarse/fine

MZ-Series Long / Short Motor Focus Columns (10 446 041 and 10 446 176

FluoCombiTM attachment for fluorescence stereomicroscope with coded

objective positions

Fluorescence objective revolver with coded objective positions

MZ16 A and MZ16 FA

Z6 APO A and Z16 APO A, motorised Macroscopes

Z6 APO, Z16 APO, manual Macroscopes

IsoProTM motorized XY stage

Configuration of UMC

Configuration of new footswitch

MZ16 F, MZ16, MZ12.5. MZ10 F, MZ9.5, MZ7.5, MZ6

DMS300, DMS1000

LAS System Requirements Page 2

 DMS300 Cleanliness Expert, DMS1000 Cleanliness Expert S6 D, S8 APO

VZ85, VZ85 R, VZ85 C, VZ85 RC, VZ105, VZ105 C, VZ15 M

EZ4 D, EZ4 HD, EZ4 W, EZ4 E

MacrofluoTM

Fluocombi / Revolving nosepiece

Support by USB for TL RCI and IsoProTM motorized XY stage

Support for USB / RS 232 serial adapter (11 532 295)

Transmitted light base for Stereo Microscopes TL4000 RCITM

Transmitted light base for high-end Stereo Microscopes TL5000 Ergo

External light sources (Photonic CLS 150XD, CLS 150LS, CLS150 LED, KL1500
LED Plus, KL2500 LCD, KL2500 LED, EL6000)

Oasis-blue XY controlled stages with Z series, M series, DVM2500

LMT260 XY Scanning Stage (for TL4000 and TL5000 Ergo requires

appropriate adapter)

LAS System Requirements Page 3

 Leica Digital Microscope Cameras
This release only supports the following Leica Digital Cameras on a Windows
PC meeting the requirements detailed in Section 2:

FireWire A FireWire B USB3.0

USB2.0
(1394a) (1394b)
DFC280 DFC295 IC80 HD DMC29001

DFC290 DFC290 HD ICC50 DFC3000G2

DFC320 R2 DFC310 FX ICC50 HD DFC7000T2

DFC300 FX R2 DFC345 FX ICC50 W DMC45003

DFC340 FX DFC360 FX ICC50 E

DFC350 FX R2 DFC365 FX MC120 HD

DFC420 DFC400 MC170 HD

DFC420 C DFC425 MC190 HD

DFC480 R2 DFC425 C EC4

DFC490 DFC450

IC D DFC450 C

IC 3D DFC495

DFC500 DVM2000

DVM2500

DFC550

Note 1) DMC2900 will work with a USB2.0 interface

Note 2) DFC3000G & DFC7000T requires a USB3.0 interface and will not work
with USB2.0 interfaces.

Note 3) DMC4500 will work on USB 2 Interfaces but with only 1 image format
available (Full Frame)

The Web Sharing module does not support USB 2 or USB 3 cameras

LAS only supports the cameras listed in the table above. Other cameras are not
supported

Multiple Cameras with LAS:

LAS supports the use of multiple Leica cameras, with the same interface
type, connected to the same system. FireWire A and FireWire B cameras
can be used together. LAS does not support mixing FireWire and USB2.0 or
USB 3.0 interface cameras on the same system at the same time. Note:
only one camera can be active at a time.

Multiple Leica DFC500 and DFC550 cameras connected to the same system
are not supported

LAS System Requirements Page 4

 2 Computer System (PC) Specification
Leica Software Products using this Specification
This PC specification also applies to the following software products with the
restrictions mentioned:

LAS V4.7

Leica Map Start and Premium

For all other software products, please refer to their individual

specifications.

Windows Operating System

Windows 8 and Windows 8.1 Pro, 32 or 64 bit

LAS requires .NET3.5. Windows 8 does not install .NET3.5

automatically. If .NET3.5 is not installed a message will be
displayed when trying installing LAS.
Click on the link below to go to the Microsoft website for
instruction on downloading and installing .NET 3.5 on the Windows
8 operating system

http://msdn.microsoft.com/en-us/library/hh506443.aspx
Windows 7 Professional or Ultimate, 32 or 64-bit.

Check Cameras section for specific Windows OS support.

Windows XP is not supported

Windows 10 is not supported

Microsoft Office

Where applications require Microsoft Word or Excel, then Office 2003 or later
must be used.

Multi-lingual support:

Chinese, Japanese and Korean require Windows 7 Ultimate.

Windows 8 may require the download of a language pack either direct from
the Control Panel or via the Microsoft Download Center

Windows Hardware Detection Service:

The Windows Hardware Detection Service must not be disabled.

Windows Experience Index:

With Windows 7 and 8 a Windows Experience Index of 4 is required for simple

acquisition and 5 or higher when multiple images will be processed.
LAS System Requirements Page 5
 Installing the Leica FireWire driver may de-register the LAS license file. Check the LAS Installation Guide help
for re-registering guidance.

Computer Hardware Requirements

Note a PC Performance check program is included on the LAS DVD and this
should always be used before installing LAS.

The specifications listed below should be considered the minimum to ensure

smooth and reliable operation of LAS

Processor:

Intel i3 or higher with CPU clock of 2.5 GHz or faster.

For highly demanding tasks such as Montage and MultiStep it is recommended
to use a PC with a minimum Intel Core i5 processor or better.
RAM:

4 GB or more of system RAM recommended.

For optional modules and for processing large image stacks, 8 GB or more of
system RAM is highly recommended.

Hard Drive:

256 GB or higher, SATA 7200 rpm, 8MB cache, formatted as NTFS.

Approximately 2 Gbytes of disk space for a full installation and an additional

free space of 10Gbytes for efficient operation. Further space is required for
data and image storage, which depends on the application and frequency of
use. The free space must be checked regularly.

For the optional MultiStep Module it is recommended to have a minimum of an

additional 10Gb of free space.

Monitors:

19 or larger monitor set to screen resolution of 1920 x 1200 (recommended),

1600 x 1200 or 1280 x 1024.

If a monitor has yet to be purchased and a large screen area is needed, a

monitor with a resolution of 1920 x 1440 is recommended.

Dual Monitors:

LAS is configured for display on a single monitor or if dual monitors are

available they should be configured as 2 separate monitors with the desktop
from Monitor 1 extended onto Monitor 2.

Graphics:

128Mb Ram minimum for single monitor or 256Mb Ram for dual monitor.
Shared memory is not recommended. PCI Express.

LAS System Requirements Page 6

 128-bit memory bus width.

32 bits operation.

Support for Direct X Version 9c or Direct X Version 10 or later for Windows 7

and Direct X version 11 for Windows 8

The minimum graphics adaptors are the Radeon X800, ATI Fire GL V7200 and
recommended adaptors are NVidia GeForce 9800GT, ATI FirePro V480O or
equivalent using the PCI Express bus or later models.

Select High quality or Optimal quality where Direct 3D options are offered
by the graphics card and not Balanced which appears by default.

Integrated graphics cards part of the motherboard layout are not

recommended as they have reduced performance. Use of system or shared
memory by the graphic card will impair performance. Some annotation and
measurement overlays may not perform as expected.

The latest graphics drivers must be installed. Obtain these from the PC or
graphics board manufacturer, not Microsoft Corp. Not all graphics boards are
compatible with all features of LAS. If it is found that some graphics elements
do not appear or are corrupted, exchange the graphics board.

The bit-depth of the graphics display system must be set to 32 bits.

Hardware acceleration should be set to full.

External Connectors

USB2.0 (Minimum), (USB3.0 recommended for the DMC2900 camera and

required for the DFC3000G camera and DFC7000 T): is required for each of
the following application and components in simultaneous use:

USB camera.

Microscope.

Microscope additional controls.

Leica LAS optional license Dongle.

Leica Map Start or Premium Dongle.

Other Components

Keyboard and PC Mouse with wheel. The wheel should also act as a third
button.

600W PSU if all options are installed.

Full-height card slots for optional interface boards e.g. Oasis. The slot next to
the Oasis board needs to be free or at least the next board must have low
profile components.

LAS System Requirements Page 7

 FireWire controllers:

Depending on the type of camera, a powered 1394b 9-pin FireWire OHCI port
is required for use with Leica DFC cameras. As FireWire interface boards from
some manufacturers have issues with non-Leica drivers, we recommend the
use of the FireWire boards in the list below.

Please note that you will also need the FireWire -B-B cable 11 600 269, if both
camera and PC have a 1394b (FireWire -B) connector.

All DFC cameras are now supplied with a suitable FireWire -B board and
FireWire -B-B cable.

FireWire 1394B is required with Windows 7 and 8 OS.

Notebook Computers
Notebooks are normally designed for power saving rather than performance
and so it is necessary to be careful in identifying suitable configurations. A
true desktop replacements using desktop components in a movable but heavy
case is required and should be compared with the Desktop specification above.

Some Notebooks have automatic power saving features. Please be aware that
LAS cannot control these and if they become activated, the performance of LAS
will be very poor as will other programs.

Notebooks cannot be fitted with the Oasis XYZ control board so cannot be used
with LAS Power Mosaic, LAS Cleanliness Expert, LAS, when a motorised stage
or focus are to be used and are connected to an Oasis XYZ control board.

Otherwise the recommended processor is the Intel Core i7 with speed greater
than 2.0GHz. As Notebook configured processors use a slower memory bus and
slower hard-drives, the overall performance is not as good as the Desktop
equivalents.

The smaller screen size of Notebooks may not be comfortable for use with
high-resolution images. Make sure that the vertical resolution is at least 1024
lines because many Notebooks have lower vertical resolution that limits the
image size and panel display. It helps to place the taskbar on the left edge of
the screen to avoid loss of vertical resolution.

For Notebooks it is important to ensure that the display does not use an
integrated graphics controller. This will severely restrict live image
performance. A dedicated and modern graphics controller is needed such as
NVidia Quadro NVS 160M or later.

LAS System Requirements Page 8

 3 Power Mosaic and MultiStep Components
Supported Components:

12 730 074 LAS MultiStep

12 730 181 LAS Power Mosaic
12 730 182 LAS Power Mosaic Plus
11 561 104 Oasis-blue XYZ kit for Power Mosaic
12 730 195 Oasis-blue Encoder kit for Power Mosaic
12 730 196 Mrzhuser Scanning Stage, 130x85mm, 2mm with Encoders
12 730 197 Mrzhuser Scanning Stage, 130x85mm, 2mm
12 730 198 Stage insert for one slide for SCAN 130 x 85
12 730 199 Stage insert for four slides for SCAN 130 x 85
Mrzhuser Scanning stage 100x100 mot., for DM4000-
11501260
6000, S 2mm
11501262 For 11501260 - Stage insert for 2 slides 3x 2 (76 x 52 mm)
11522605 Mrzhuser Scanning stage 75x50, 2mm incident light only
11600183 For 11522605 - Glass plate 160x116x3mm
11532502 For 11522605 - Metal insert 160x116
11600184 For 11522605 - Holder for glass slides 3"x1"
11600185 For 11522605 - Holder for slides frames 70x70mm
For 11522605 - Stage insert 160x116x7mm (for Cleanliness
11532701
Expert filter holders

Some Oasis boards may not be correctly configured for use with Power Mosaic
pattern navigator. If Power Mosaic Plus is installed and pattern navigator does
not appear, contact DI.Support to get the Oasis card license code updated.

Supported Microscopes:

Only the microscopes listed are suitable for use with Power Mosaic and
MultiStep:

DM 5500 B, 6000 B/M.

DM 4000 M - With addition of external focus motor.

Generic microscopes with motor stage and motor focus.

Macroscopes Z series, Stereomicroscopes M series and DVM 2500.

DMS 300 and DMS 1000 (MultiStep only)

DM 12000, 8000 motorised version only, no Turboscan, no Oasis board

required

LAS System Requirements Page 9

 Supported Cameras:

Only the cameras listed are suitable for use with Power Mosaic: Turboscan
and Standard scan modes and MultiStep:

DFC400, DFC300FX, DFC310FX, DFC350FX, DFC360FX - Requires trigger cable

included with Oasis-blue kit.

MultiStep and Standard scan only:

DFC290, DFC295, DFC420, DFC425, DFC450, DFC450C, DVM2500 - Do not use

a trigger cable.

The Set Shading feature sometimes fails with these cameras, please contact
Di.Support for advice.

Hardware:

12 730 193 Oasis-blue Controller board and Cable Kit for stepper stages
includes:

XY Cable with Mrzhuser Round Connectors

Z axis and DFC Trigger Cable split cable
Smart Move or Joystick I/F Cable
MIC Box Pass-Thru (cable / ribbon cable / plate)
Focus adapter for Mrzhuser /DM6000/ Prior focus drive.

Motorized Stage with Stepper Motors:

Please refer to the table. Note: At least one stage insert is also required.

The Smart Move controls the stage and focus: The focus knob on the
microscope stand is disabled when configured for Turboscan..

11 505 180 Smart Move for DM/DMI Series.

Other scanning stages may also be used but suitability must be confirmed in
advance of use.

Motorized Focus:

DM5500-6000 use internal focus motor driven by Oasis using CTR MIC
control cable with Z breakout.

11522607 External motorized fine focus for DM2500M/P / DM2700M/P

11522608 External motorized fine focus for DM4000M and DM4500P

11522606 External motorized fine focus for DMI5000M and DMI3000M

Encoder Option:

12 730 195 Oasis-blue Encoder kit for Power Mosaic used where best
accuracy is required with high-mag objectives.

Includes daughter board that fits on the Oasis-Blue board and modified XY
Cable for Encoder Inputs.
LAS System Requirements Page 10
 Requires 12 730 196 Mrzhuser Scanning Stage 100 x 80mm 2mm with
Encoders.

LAS System Requirements Page 11

 4 Factors that may reduce performance
Users should be aware that deviating from the hardware and software
recommendations may affect Leica Application Suite performance. The
following is a list of situations to be avoided; it is not exhaustive.

Operating systems other than the Windows-based versions listed.

Integrated graphics, AGP graphics, graphics that share CPU memory and
using a mobile graphic device.

Displays on dual monitors with low performance graphics card.

Monitors with less than 1024 vertical resolution.

Less than 400 MB free RAM before LAS starts. 700MB is required for LAS
MultiFocus and LAS MultiStep and 1 GB for LAS Power Mosaic. Installed
RAM of 4 GB or more RAM is required.

Other programs running at the same time as LAS.

Using any other than the recommended Intel i3 or higher range.

Using system power saving.

Using sleep, hibernation, and auto power-down modes.

It is recommended to disable sleep, hibernation, and auto power-down

modes when using LAS as it has been found on some systems that the
application does not recover properly, especially when using sequence
modules such as Multi-Time, Multi-Step.

Personally constructed personal computers.

An environment that is an upgrade of the original manufacture-installed

operating system.

Multi-boot environments.

Shadow or Animated cursors and UI graphics.

Hyper threading is not expected to improve performance.

Saving images on a remote or server computer.

Anti-virus/Anti-Malware applications running whilst LAS is in use

LAS System Requirements Page 12

Leica Microsystems (Switzerland) Ltd.
Stereo and Macroscope Systems
CH 9435 Heerbrugg
Switzerland

Support:
www.leica-microsystems.com/contact/service
www.microscopy-imaging.com