T cell proliferation and the immune response

Antigen presenting cell

Ag
Calcium channel
CD4/CD8

+P +P T cell

+P

+P

+P

+P

Apoptosis
+P
+P

Cell growth
+P

+P
+P

+P
+P

-P

+P +P

+P
+P

+P +P
+P
+P

Immune response Isolate

T cell proliferation

Dynabead
Higher
Release

Isolate Stimulate Analyze

No cell

Product Cat. no. Product Cat. no. Product Cat. no.

Dynabeads FlowComp Human CD4 113-61D OpTmizer T Cell Expansion SFM A1048501 Click-iT EdU Pacific Blue Flow Cytometry A10034
Dynabeads FlowComp Mouse CD4 114-61D AIM V Medium Liquid 0870112DK Assay Kit

AIM V Medium Liquid (research grade) 12055083

Dynabeads FlowComp Human CD3 113-65D Human IL-4 ELISA Kit KHC0041

Dynabeads FlowComp Human CD8 113-62D Dynabeads Human T-Activator CD3/CD28 0.4 mL 111-61D Human IL-6 ELISA Kit KHC0061

Dynabeads FlowComp Mouse Pan T 114-65D Dynabeads Mouse T-Activator CD3/CD28 0.4 mL 114-56D Human IL-10 ELISA Kit KHC0101
Human IFN-gamma
Stimulate KHC4021
Dynabeads FlowComp Mouse CD8 114-62D
For more information about T-Activator, visit www.invitrogen.com/t-activator. Human TNF-alpha KHC3011

Dynabeads Untouched Human T Cells 113-44D

Human IL-2
Isolate PHC0026 View our extensive portfolio of ELISA and Luminex assays at
Dynabeads Untouched Human CD4 113-46D
www.invitrogen.com/immunoassays.
Dynabeads Untouched Human CD8 113-48D Mouse IL-2 PMC0024
Dynal Mouse T Cell Negative Isolation Kit 114-13D Human IFN-gamma PHC4031 OpTmizer
CellTrace CFSE Cell Proliferation Kit C34554
Dynal Mouse CD4 Cell Negative Isolation Kit 114-15D Mouse IFN-gamma PMC4034 Superio
SYTOX Red Dead Cell Stain S34859 Serum-
Dynal Mouse CD8 Cell Negative Isolation Kit 114-17D Human TNF-alpha PHC3015
Dynabeads FlowComp isolation
SYTOX AADvanced Dead Cell Stain S10274 Cost sa
Mouse TNF-alpha PMC3014
Human CD3-488
Higher purity and reproducibility MHCD0320
Human Regulatory CD4+CD25+ T Cell Kit 113-63D Human IL-4 PHC0044 Release and remove the beads
No cell activation
Mouse CD3-488 HM3420
FlowComp Mouse CD4+CD25+ Treg Cells 114-63D Mouse IL-4 PMC0045
Human CD4-PE MHCD0404
Human IL-6 PHC0065
Mouse CD4-PE MCD0404
Dynabeads FlowComp Flexi 110-61D Mouse IL-6 PMC0065
Vybrant LIVE/DEAD Fixable Aqua Dead Cell Stain L34957
Human IL-10 PHC0105
Vybrant LIVE/DEAD Fixable Violet Dead Cell Stain L34955
For an overview of Dynal products, visit www.invitrogen.com/dynabeads. Mouse IL-10 PMC0104
Vybrant LIVE/DEAD Fixable Red Dead Cell Stain L23102

Isolate For a full listing of growth factors, visit

For a full listing of flow cytometry reagents, visit
www.invitrogen.com/growthfactors.
www.invitrogen.com/flowcytometry.

Stimulate Analyze
250

S/N = 13
200
Number of cells

Dynabeads FlowComp isolation

150
Higher purity and reproducibility OpTmizer SFM/AIM V cell culture Click-iT E
Release and remove the beads Superior performance 100 No mor
No cell activation Serum-free expansion
Cost savings
Simple
50
P2
P3 Easy m
0
0 102 103 104 105

Click-iT EdU Pacific Blue

fluorescence

Analyze
Stimulate 250

S/N = 13
200
Number of cells

150
Click-iT EdU
2009 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. These No catalog
100 products may be covered by one or more Limited Use Label Licenses (see Invitrogen moreor www.invitrogen.com).
painful BrDU By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.
For research use only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated. P-083505 0409
Simple protocol
50
P2
P3 Easy multiplexing