Earl John E.

Caonero

Laboratory Exercise No. 9

Culture and Microscopic Examination of Fungi

1. Define budding and hyphae.

Hyphae is a long filament of fungal cells or actinomycetes, and budding is an asexual

reproduction that begins as a protuberance from the parent cell and grows to become a daughter
cell (Tortora et al., 2016)

2. Why do yeasts generally have to be cultured for longer periods than most bacteria?

Fungi are eukaryotic protists which are more complex and differ in many ways with bacteria
such as in the time it needs to culture fungi. Bacteria reproduce rapidly through binary fission,
while yeasts have a lengthier generation time (Kumar, 2012).

3. What are some similarities and differences of the yeasts that were cultured from your
mouth?

N/A

4. What is the difference between vegetative and aerial mycelia?

A mycelium is constituted by a tangled mass of hyphae that forms in molds or filamentous fungi.
A vegetative mycelium is a hypha that penetrates the supporting medium and absorb nutrients,
while aerial hyphae project the surface of the mycelium and bear the reproductive structures of
the mold which produces asexual pores (Kumar, 2012).

5. Can bacteriological media be used for the cultivation of molds? Explain your answer.

Yes. Most fungi occur in nature and grow readily on simple sources of nitrogen and
carbohydrate, so bacteriological media that contain glucose and modified peptone with a neutral
pH may also be used to cultivate molds.

References:

Brooks, G., Carroll, K., Butel, J., Morse, S., & Mietzener, T. (2013). Jawetz, Melnick and
Edelberg's Medical Microbiology. . 26th ed. The McGraw Hill Companies, Inc.
Kumar, S. (2012). Textbook of Microbiology. Jaypee Brothers Medical Publishers.
Tortora, G., Funke, B., & Case, C. (2016). Microbiology An Introduction. Pearson Education,
Inc.