4/20/2017 PhysiologicalandChemicalPropertiesofAnimalCellCultureMedia

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PhysiologicalandChemicalPropertiesofAnimalCellCultureMedia
BY:NidhiUppangala|Category:Others|Submitted:2010083112:04:31

Article Summary: "The ideal animal cell culture medium is expected to possess certain
physiological and chemical properties such as pH, oxygen, carbon dioxide, buffering,
osmolality, viscosity, temperature in such as way that it should support growth and
proliferation of cultured animal cells..."
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Introduction:

TheidealanimalcellculturemediaisexpectedtopossesscertainphysiologicalandchemicalpropertiessuchaspH,oxygen,carbondioxide,buffering,
osmolality,viscosity,temperatureinsuchaswaythatitshouldsupportgrowthandproliferationofculturedanimalcells.

pH:

NormallymostoftheanimalcellscangrowatpH77.4,althoughtheremaybesomeslightvariationsintherequiredoptimumpHdependingonthetype
Start
andoriginofcells.ThepHindicatorphenolredismostcommonlyusedforvisibledetectionofmediapH.
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Carbondioxide,BicarbonateandBuffering:
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1.Carbondioxideintheanimalcellculturemediaisinadissolvedstateanditsconcentrationdependsonthetemperatureandalsotheatmospheric
carbondioxidetension.Carbondioxideinthemediumexistsinmanyformssuchascarbonicacid,HCO3andhydrogenions.

2.Concentrationofcarbondioxide,bicarbonateandpHareinterdependent.Thatisforexamplebyincreasingtheconcentrationofatmosphericcarbon
dioxidedecreasesthepHofthemedium,thisinturnwillmakecellculturemediumacidic.Additionofsodiumbicarbonateasbicarbonatebuffer
neutralizesthebicarbonateions,whicharepresentinthemedia.
Convert From D
3.Thepresenceofpyruvateintheanimalcellculturemediaincreasestheendogenousproductioncarbondioxidebythecells.
to PDF, PDF to D
Oxygen: Simply With Th
Free Online Ap
1.Theproliferationandscaleupofanimalcellculturedependsupontheoxygensupplywithoutcausingcelldamage.

2.Oxygenissparinglysolubleincellculturemedia.Butculturedcellsdependonthedissolvedoxygenpresentinthemedia.Buthighconcentrationofthe
samemayactastoxictotheculturedcellsduetothegenerationoffreeradicals.Thereforeoxygenmustbesuppliedinsuchawaythatitfullfillsallthe
cellrequirementsandavoidstoxiceffectsonculturedanimalcells.

3.Freeradicalscavengerssuchasglutathioneareusedtonullifythetoxiceffectcausedbythehighconcentrationofoxygen.

4.AdditionofSeleniumtotheculturemediumalsoreducestheoxygentoxicity,asseleniumactascofactorintheproductionofglutathione.

5.Culturedcellswhencomparedtoinvivocellsundergoanaerobicformofglycolysisandalsothedepthofcellculturemediainfluencetherateof
oxygendiffusion.

Temperature:

1.Animalcellsaremoresensitivetotemperaturehigherthanthebodytemperature,thatis37c.Butdependingontheoriginofculturedcellsoptimumtemperaturevaries,
examplecellculturefrombirdsisaround38.5candcellsculturedfromcoldbloodedanimalsisaround1525c.

2.Animalcellculturemediahaslowthermalconductivity,hightemperaturegradientcanoccurwithheatingandmixingofthemedia.

3.Inasmallorcompactbioreactorlowgradientheatingsystemcanbeachievedusingathermostateddraughttube.

4.Temperatureoftheculturemediainfluencestheanimalcellgrowth,andalsotemperaturealsoaffectstheoxygensolubility,thatishighertemperatureincreasesthesolu
ofoxygen.

Osmolality:

http://www.biotecharticles.com/OthersArticle/PhysiologicalandChemicalPropertiesofAnimalCellCultureMedia373.html 1/3
4/20/2017 PhysiologicalandChemicalPropertiesofAnimalCellCultureMedia
1.Osmolalityformostoftheculturedanimalcellsisaroundintherangeof260320mosm/kg,comparabletotheosmolalityofhumanplasma,whichisaround290mosm/k

2.Oncetheosmolalityforacellculturemediaisselected,thenitmustbemaintainedatthatlevelthroughouttheculture.

3.Additionofacids,bases,drugsoranyothercompoundstothecellculturemediumalterstheosmolalityandthesameismeasuredusingOsmometer.

GrowthFactors:

Growthfactorspromoteinvitroproliferationanddifferentiationofculturedcells.Growthhormonessuchasinsulinandhydrocortisonearemostcommonlyusedinserumfr
artificialanimalcellculturemedia.Forexamplecombinationofsteroidhormonessuchashydrocortisone,estrogenandalsoandrogenareusedinmaintainingthemammar
epithelialcellsinthelaboratory.

Nutrients:

Animalcellculturemediaalsocontainsnutrientssuchascholin,ethanolamine,linolicacid,andalsomineralslikeiron,copper,seleniumandmuchmoreareadded,insuch
waythatcombinationofallthesenutrientspromotetheproliferationofculturedanimalcells.

Proteins:

MostcommonlyaddedproteinintoanimalcellculturemediaisBovineSerumAlbumin(BSA).Thisproteinpromotesthecellgrowthanditalsoincreasesthesurvivalrateo
animalcells.
Likewisepolyaminesandproteaseinhibitorsarealsoaddedintotheanimalcellculturemedia.

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memyself
dissolvedoxygencapacitygoesdownwithincreasingtemperature
2011031110:11:14141

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