Solid Phase Peptide Synthesis (SPPS)

Synthesis and Analysis of Organic and Inorganic Compounds

(CH3102)
May 29, 2017

Jiwon Seo
Department of Chemistry
SPPS RGDS synthesis

1. final cleavage and deprotection

2. ether precipitation (why not methanol precipitation?
DCM? ACN?)
 Chromatography
mobile phase: eluent system
stationary phase: column packing resin
=> normal phase (silica gel) / reversed phase (C18)

menadione

tocopherol
C18 column (reversed-phase HPLC)

injection peak
Amide column (normal phase HPLC)

nonpolar polar
ChemBioDraw is a powerful and widely used software for chemists and biologists.
While you analyze your ESI-MS data, ChemBioDraw will help you a lot.

GIST has a license for this software. Everyone who has gist.ac.kr email address
can download it.

1. Go to http://its.gist.ac.kr/downloadable_software
(GIST IT service/software download)
2. At the bottom of the software list, you will find the ChemDraw icon.
3. Click and login to the GIST server. Follow the installation instruction.
ACS format
Show analysis window
Analysis window
modify and select => mass changes in analysis window
fragmentation tools

mass of each fragment



SPPS (RGDS) ESI

: 432.21
Solvent: ACN/MeOH/H2O
2012.11/27~12/6

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