AlleleBalanced

Luciferase Assay Kit 9924 Mesa Rim Road. San Diego, CA 92121
tel: 858-587-6645, 800-991-RNAi
fax: 858-587-6692
website: www.allelebiotech.com
email: oligo@allelebiotech.com

AlleleBalanced Luciferase Assay Kit Storage conditions: -20 oC for 6 Months (With or
100 Reactions ABP-PA-ABLA001 Without Lysis Buffer)
1000 Reactions ABP-PA-ABLA011
Temporary Storage: 4 oC In Sealed Bottle for 1 Day

Description: Kit Contents

AlleleBalanced Luciferase Assay Kit is a novel for- 100 reactions

mulation for measuring fire fly (Photinus pyralis)
luciferase gene (luc) expressions in mammalian Bottles Description Size
tissue culture cells or in bacteria. 1 Luc Assay (3x) 10ml
1 Lysis Buffer (3x) 10ml
Resulting from Allele Biotech’s extensive research
and development efforts, this brand-new as- 1000 reactions
say system offers extremely sensitive detection
tools for those using luc as reporter. It provides a Bottles Description Size
simple reaction system balancing sensitivity and
stability to suit most routine Luciferase based gene 1 Luc Assay (3x) 100ml
expression analysis. The kit can be used in low or 1 Lysis Buffer (3x) 100ml
high throughput applications at a much reduced
cost. The AlleleBalanced Luciferase Assay Kit is very
easy to ship, store, and use. Recommended Protocol:

Features: 1) Cell preparation

-High sensitivity, gives more signal than compared For attached or suspension culture cells or bacte-
leading brands rial cultures, simply add the same volume of Lysis
Buffer to the cells in medium. Attached cells will
-Stability, offers maintenance of more than 95% be dislodged while being lysed.
signal level after 3 hours
2) Luc Assay
-Easy to use, simply lyse the cells, mix, and read
Add Luc Assay reagent in equal volume to the Lysis
-Low cost shipping, the reagents are stable for a Buffer, take reading. The signals may continue to
few days at room temperature, eliminating the rise slightly during the first 60 min, then stabilize
need for cold packaging. during the next 2 hours, providing excellent time
frame for high-through put assays or long term
-Easy storage, -20 oC is recommended for long time course studies.
term storage; repeated freezing and thawing will
not affect its effectiveness

Applications: Quality Assurance:

Suitable for molecular and cellular studies: Each batch of the reagents has been tested for
-Enhancer/promoter analysis with luc
as a reporter gene performance and consistency using purified Luci-
-Enzymatic screening assays ferase and mammalian tissue cells expressing luc
-Control experiments using Luciferase for gene.
transgene expression, RNAi

License
The purchase price grants end users a non-transferable, non-exclusive license to use the kit and its components for internal research use only. Research use excludes resale, repackag-
ing, or selling of any commercial product without the written approval of Allele Biotechnology & Pharmaceuticals, Inc.. Products are not to be used for human diagnostic or used in
any drug intended for human use. By keeping or using the enclosed materials, you agree to be bound by the terms of this license. The laws of the State of California shall govern the
interpretation and enforcement of the terms of this license.