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Ru ic, R. Jerman, I. , karja, M.,Mogilnicki L.
BION, Institute for Bioelectromagnetics and New Biology; Stegne 21, 1000 Ljubljana , Slovenia .
Tel/Fax: +386 (0)1 513 11 47,
e-mail: igor.jerman@bion.si, romana.ruzic@bion.si, metod.skarja@bion.si


In this report we present our testing on a hypothesis that biologically relevant information from
various substances can be nonchemicaly translated to the organisms by high voltage electric
and/or magnetic fields that imprint the information into water or some solution. The stored
information under suitable conditions triggers a specific biological response without any
chemical contact with the original substance. We constructed a special device whereby various
experiments - using our well explored biological sensor system - were performed. The biological
effectiveness of the information transfer of various configurations and types of electric and
magnetic fields as well as three different biologically active substances were tested. The results
showed that even by the electric field informed alcohol solution has statistically significant
biological effects, as well as the information from some of a chosen chemical substance,
especially herbicide.

1. Introduction

More and more researches confirm biological effects of magnetically treated water (1 -3) but the
existence of the so called water memory is still an object of an intensive debate because there
is still no valuable theory that would explain, how biologically effective information can be stored
in water. One hypothesis predicts changes in the water structure by forming more or less
permanent clusters (2,4), the others point to the unusual properties of water under certain
treatments; various thermodynamics methods were used (6,7). Some physicists proposed that
one way, in which memory' might be stored in water, is through the collective motions of water
dipoles, which have greater polarisability than the solitary ones. External sources with low-
frequency polarisation field (for example other molecules) can be imprinted into water by
modulating the fundamental frequency of the latter (8). Beside physics that tried to explain this
phenomenon, various biological effects of such "informed" water were published as well as
experiments with regard to the biophysical properties of the information transfer process (9-12).

In the present work we based on the idea that: a) from high voltage electric field information
(with or without an accompanying magnetic field) can be imprinted into water; b) that such an
electric field can further imprint the biologically active information of any substance into water
(or some solution); c) that such "informed water would have measurable biological effects. So
we constructed a special electromagnetic device (see Scheme 1) and performed many
experiments to tests the biophysical properties of the information transfer by using our well
tested biological (plant) sensor system. This system consists of germinating cress seedlings
exposed to the well defined heat stress. Originally the system was developed for testing the
biological effects of very weak magnetic fields of various types and produced statistically
significant results (5).

2. Material and methods

2.1. Instrumentation

To perform an imprinting the chosen information into water correctly, we constructed a system
consisting of a high voltage source and a wire that is installed into a quartz test tube with a
biologically active (donor) substance. The quartz test tube is placed into ordinary glass test tube
filled with water solution of ethanol (i.e. 60% ethanol; acceptor). Under this condition an almost

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absolute chemical isolation between the donor and the acceptor is achieved (for details see
Scheme 1). For the control we use bidistilled water as the donor.

Scheme 1: System for information transfer

2.2. Plant sensor system and the experiments

The plant sensor system consisted of. Lepidium sativum (cress seeds), which germinated in
Petri dishes (8 x 30 seeds for one experiment) on the filter paper wetted with bidistilled water.
After 24 hours the plants were exposed to heat stress at 42 C for 40 min (to enhance the
susceptibility of plant organism to the environmental factors). In these experiments two types of
cress seeds were used, one with 50-55% (low) and another with 90-98% (high) germinability.

The effects of the information from the following (donor) compounds were tested: alkaloid
amanitin, herbicide Glyphosphate and cytokinin Benzylaminopurine (BAP). The prepared
informed solution was further twice diluted with bidistilled water in the ratio 1:100, while between
consecutive dilutions the new diluted solutions were agitated (succused) 100 times. With this
final solution the seeds were moistened 3 ml per Petri dish. The growth properties of this plant
sensor system was also tested by moistening the seeds with ordinary distilled water and with
the solution of different concentrations of donor compounds without any informing process. The
results were used for comparison. The protection gloves were used during the whole procedure.

During the germination the Petri dishes were covered with an aluminium foil and grew in dark.
The seedlings were grown in a thermally isolated box at room temperature. Some experiments
with amanitin were also performed at varied temperature conditions. We also performed the
control experiments where procedures were the same except that ethanol solution was not
exposed to EM field. Some preliminary experiments with pure water and water solution with the
Fe and Cu ion solution instead of ethanol (in conc., 3.7, 18.5 or 37 M as FeCl3.6H2O and 0.012
M CuSO4.5H2O) were also performed.

2.3. Analysis of Data

Every experiment lasted 48h, than the length of radicles was measured, followed by the
statistical evaluation of parameters and significance used by Student t-test for comparison
between two groups. The significance is marked on figures as follows: * p<0.05, ** p<0.01 and
*** p<0.001.


3.1.Preliminary experiments

Preliminary experiments with information of amanitin showed that using pure water for
imprinting instead of ethanol solution did not produce effects. When using water mixture of
added Fe and Cu ions, stimulative 5-12% statistically significant effects were obtained at

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concentrations 3.7 and 37 M Fe plus 0.012 M Cu (same for both). The stimulative effects
decreased with the duration of stored solution (after 5 days no effects were obtained any more).

For better longevity and stability of the stored information a mixture of ethanol and water was
used in further experiments that are still going on.

3.2. Electric field alone

Experiments using the electric field and a bidistilled water as source of information produced
inhibitory effects on the plant sensor system. The average inhibition amounts to approximately
8%, is highly stable and statistically significant. Since the system is exposed to the heat stress
this means that the exposure of the plant sensor system to electric field treated solution
enhanced the stress effect of heat. Comparison with the effects of other compounds, showed,
that in most cases, the informed solution inhibited the growth reaction of plant sensor system at
a similar level; the difference against ordinary (not informed) solution was highly significant (see
Fig.1). It is also important to note that these results were obtained with the seeds of high
germinability. Of course, these results comprise the data of all experiments together, which
minimise the variations between experiments, the next figures show up the detailed situation.

Fig. 1 The effect of informed in comparison with not informed solution

on the growth reaction of plant sensor system (seeds with high germinability) - all experiments together

3.3. Experiments with amanitin

Some preliminary experiments showed that the information "amanitin" had stimulative effect in
comparison with information "water". Later it was found out that this effect was closely
correlated with the length of seedlings, which was very short at this result. At shorter average
lengths (less than 10 mm) the effect was positive, over 10 mm there was no effect or it was
inhibitory (Fig. 2).

Fig.2. Variation of the effect of information "amanitin" depending on the average length of seedlings low
germinability seeds.

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Further experiments performed under moderate room temperatures showed the inhibitory effect
of information "amanitin" in comparison to information "water". The inhibitory effect of
information "amanitin" was revealed in high as well as in low germinability seeds. The results
were highly statistically significant in high germinability seeds but not in the low ones (see Fig 3)

Fig.3. The comparison of the effect of amanitin (as information) between low (LG) and high germinability (HG)
seeds .

More experiments (see Fig. 4) performed with high germinability seeds showed, that both
information "water" as well as information "amanitin" produced inhibitory effects compared with
ordinary distilled water (first two columns), the solution of amanitin itself (in conc. 0.5-2%) did
not influence the growth of seedlings. Information of "amanitin" had stronger inhibitory effect
then information "water" and much stronger effect than the diluted amanitin itself (!).

Fig. 4. The effect of information "water", information "amanitin", amanitin solution and
comparison between themselves on the growth reaction of plant sensor system .

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3.4. Experiments with herbicide

The herbicide solution normally inhibits the growth of plants. When low germinability seeds were
used the ordinary solution of the herbicide had the strongest inhibitory effect (around 36%),
information "herbicide" had lower but still statistically significant inhibitory effect (Fig. 5).
However, there is no significant difference between the effects of information "herbicide" and
information "water" (Fig. 5). But when high germinability seeds were used, information
"herbicide" had more potent inhibitory effect than herbicide solution itself. The difference
between information "herbicide" and information "water" was also highly statistically significant

Fig. 5. The effect of information "water", information "herbicide", herbicide solution and comparison between
themselves on the growth reaction of plant sensor system low germinability seeds.

Fig. 6. The effect of information "water", information "herbicide", herbicide solution and comparison between
themselves on the growth reaction of plant sensor system high germinability seeds.

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3.5. Experiments with a cytokinin

These experiments were performed only with high germinability seeds. The chosen cytokinin
solution normally inhibited the growth of plants for about 25%. The information "cytokinin" was
less effective and not distinctive from the effects of information "water". However, the effects of
both differ from ordinary water (Fig.7).

Fig. 7. The effect of information "water", information "cytokinin", cytokinin solution and comparison between
themselves on the growth reaction of plant sensor system.


According to the results there is a strong indication that a high electric field can non-chemically
transmit information at least of itself, which is in accordance with previous studies about the
biological effects of magnetically treated water. In this research biological effects were
consistently and repeatedly revealed almost always when the imprinting procedure was used. If
this field can transmit even the information of other chemical substances, it is as yet unclear. In
some experiments, for example with a herbicide, the answer is yes, in other experiments the
effects of information "water" was not distinct from information "chemical compound". It seems
that the effects depend on the average length of seedlings as well as on an internal
physiological state of the organism. They vary through the germination time and depend also on
the seed germination quality. Further experiments are in progress.


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