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Received 16 March 2001; Revisions requested 10 April 2001; Revisions received 9 May 2001; Accepted 9 May 2001
Abstract
A model has been developed for the fermentation of mixtures of glucose and xylose by recombinant Zymomonas
mobilis strain ZM4(pZB5), containing additional genes for xylose assimilation and metabolism. A two-substrate
model based on substrate limitation, substrate inhibition, and product (ethanol) inhibition was evaluated, and
experimental data was compared with model simulations using a Microsoft EXCEL based program and methods
of statistical analysis for error minimization. From the results it was established that the model provides good
predictions of experimental batch culture data for 25/25, 50/50, and 65/65 g l1 glucose/xylose media.