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Citadel

TISSUE PROCESSOR

ENGLISH
Issue 6

OPERATOR
GUIDE
69810040

69810040 Issue 6
SYMBOLS
The following symbols and conventions are used throughout this manual and on the
instrument.

THIS SYMBOL WARNS YOU THAT YOU MUST FOLLOW INSTRUCTIONS


FOR SAFE AND CORRECT OPERATION. IF THIS SYMBOL APPEARS ON THE
INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.

THIS SYMBOL WARNS YOU THAT SURFACES ARE HOT. IF THIS SYMBOL
APPEARS ON THE INSTRUMENT, ALWAYS REFER TO THIS OPERATOR
GUIDE.

THIS SYMBOL WARNS YOU THAT THERE MIGHT BE A BIOHAZARD


ASSOCIATED WITH THE INSTRUMENT. ALWAYS ACT WITH COMMON SENSE
AND BE AWARE OF THE SAMPLES USED. TAKE SUITABLE PRECAUTIONS.

THIS SYMBOL WARNS YOU THAT HARMFUL CHEMICALS ARE USED WITH
THE INSTRUMENT. REFER TO THE MATERIAL SAFETY DATA SHEETS FOR
THE CHEMICALS USED. ALWAYS ACT WITH COMMON SENSE AND BE AWARE
OF LOCAL LABORATORY PROCEDURES. TAKE SUITABLE PRECAUTIONS.

A warning is given in the document if there is a danger of personal injury or


WARNING
damage to samples or equipment.
Note
1 Notes give more information about a job or instruction but do not form part of the
instruction

Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company


Thermo Fisher Scientific is the trading name of Thermo Shandon Limited

All information contained in this manual is proprietary and confidential, and the exclusive property
of Thermo Fisher Scientific. This manual is protected by copyright and reproduction is prohibited.
This manual is for use only by the individuals to whom it has been made available by Thermo
Fisher Scientific.

The Citadel meets the following CE Mark requirements:


In Vitro Diagnostic Directive 98/79/EC
Machinery Directive 2006/42/EC.

Thermo Fisher Scientific makes every endeavour to ensure that the information contained in its support documentation is correct
and clearly stated but does not accept responsibility for any errors or omissions. The development of Thermo products and
services is continuous. Make sure that any published information that you use for reference is up to date and relates to the status
of the product. If necessary, check with Thermo or your local Thermo representative.

2009 Thermo Fisher Scientific. All rights reserved.


This manual may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted to any
electronic or machine readable form without prior written consent of Thermo.

2 69810040 Issue 6
LIST OF CONTENTS

WELCOME..............................................................................................................5

INTRODUCTION.....................................................................................................7
1.1 SAFETY FEATURES..............................................................................8
GENERAL DESCRIPTION.....................................................................................9
2.1 MAIN BODY............................................................................................9
2.2 OPERATING HEAD ASSEMBLY..........................................................10
2.3 HAND-HELD CONTROLLER...............................................................10
2.4 OPTIONAL ACCESSORIES................................................................10
INSTALLATION.....................................................................................................12

CONTROLS AND INDICATIONS..........................................................................15


4.1 HAND-HELD CONTROLLER KEYS ...................................................15
4.2 HAND-HELD CONTROLLER DISPLAY...............................................20
OPERATING CONDITIONS..................................................................................21
5.1 AUTO MODE........................................................................................21
5.2 MANUAL MODE...................................................................................21
5.3 ALARMS...............................................................................................22
PREPARATION.....................................................................................................23
6.1 GENERAL............................................................................................23
6.2 REAGENTS..........................................................................................23
6.3 FILLING THE CONTAINERS................................................................24
PROGRAMMING..................................................................................................28
7.1 GENERAL............................................................................................28
7.2 PREPARING A PROGRAM TABLE......................................................28
7.3 ENTERING A PROGRAM....................................................................31
7.4 CHECKING AN ENTERED PROGRAM...............................................33
7.5 ALTERING A PROGRAM.....................................................................34
7.6 ENTERING A PASS FACILITY INTO A PROGRAM.............................36
7.7 ENTERING A DELAY INTO A PROGRAM...........................................38
7.8 OVERRIDING A PROGRAMMED DELAY............................................41
OPERATING PROCEDURE..................................................................................42
8.1 RUNNING A PROGRAM......................................................................42
8.2 PROGRAM SEQUENCE......................................................................44
8.3 LOADING THE TISSUE BASKETS......................................................45
8.4 LOADING THE BASKETS INTO THE HANGER..................................46
8.5 LOADING THE BASKET HANGER INTO THE CITADEL.....................46

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MAINTENANCE....................................................................................................47
9.1 CLEANING AND MACHINE CARE......................................................47
9.2 WAX BATH TEMPERATURE................................................................49
9.3 DISPOSAL............................................................................................49
9.4 DECONTAMINATION...........................................................................50
TECHNICAL DATA................................................................................................52
10.1 ENVIRONMENTAL CONDITIONS...........................................................53
ACCESSORIES....................................................................................................54
11.1 CITADEL 1000.........................................................................................54
11.2 CITADEL 2000.........................................................................................55
Warranty Statement.........................................................................................56
Declaration of Conformity................................................................................57

4 69810040 Issue 6
WELCOME
All Thermo Fisher equipment is designed to accepted standards of safety. Its use does
not entail any hazard if operated in accordance with the instructions given in this guide.
However, you must obey the following safety precautions:

WAX BATH SURFACES ARE HOT (60C).

A BIOLOGICAL HAZARD IS ASSOCIATED WITH THE INSTRUMENT, BECAUSE


OF THE PRESENCE OF TISSUES, AND OF SOLVENT VAPOURS.

Take care when loading and unloading baskets and reagent containers, to
WARNING avoid trapping your fingers.

i Potentially lethal voltages above 110Vac or 50Vdc are present inside the unit. Do
not remove any access covers.

ii If any aspect of the operation or care of the instrument is unclear, ask your
Thermo supplier for advice. Incorrect use can introduce a hazard to health, or
might damage the equipment.

iii Disconnect the instrument from the mains supply before you carry out any
maintenance or manual cleaning procedure.

iv The Citadel is heavy. Seek assistance to lift or move the instrument.

v It is important that you employ normal safety standards. Always use common
sense and good housekeeping practices when operating the instrument.

vi Wipe up any spills immediately. If fluid might have spilt inside the instrument,
disconnect the instrument from the mains supply immediately by removing the
plug from the mains supply socket. Do not reconnect to the mains supply until
the instrument has been throroughly dried out and checked by a properly trained
service engineer.

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v Always comply with local regulations when handling chemicals or reagents.
Observe the precautions recommended by the supplier of any material used with
the instrument.

vi Correct maintenance procedures are essential for consistent performance. It is


important that the instrument be serviced by properly trained service personnel.
You are strongly advised to take out a maintenance contract.

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INTRODUCTION

The Citadel is a programmable, carousel tissue processor intended for use in pathology
laboratories by appropriately trained medical laboratory technicians. It is controlled by
a hand-held controller unit connected to the processor through a coiled cable. If the
processor is located within a fume or safety cabinet, the controller unit is kept outside.

You may use Hyperclean with the Citadel, to reduce exposure to solvent vapours.

Four 12-step processing programs can be programmed into the hand held controller,
and an electronic timer with a maximum setting in each step of 99 hours and 59
minutes facilitates the running of extended programs (for example, weekend programs).
A facility is also provided which allows you to introduce a delay before the processing
schedule begins.

Tissue samples are placed into cassettes which are placed in a random or organiser
tissue basket, with a lid, and then loaded on the processor. In the standard
configuration, ten reagent containers and two independently heated wax baths are
filled, as appropriate, and the processor is then programmed to rotate the operating
head and lower the tissue basket into each of the containers for a pre-set length of time.
You can apply a vacuum to the final wax bath, if a vacuum unit has been supplied with
the processor.

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1.1 SAFETY FEATURES

Various safety features are incorporated into the processor to safeguard both the
operator and the tissue specimens.

i The processing cycle stops auto


matically at position 12 to prevent double
processing.

ii The temperature of each wax bath is controlled electronically and can be adjusted
over the range 45-65C.

iii A safety cut-out device prevents the wax overheating.

iv You can raise manually the lift arm assembly from which tissue baskets are
suspended, to remove any blockages or obstructions.

v Electrical and mechanical safeguards are incorporated.

vi When power is restored after a power failure during an automatic cycle, the
processor resumes operation from the step and time of the interruption.

8 69810040 Issue 6
GENERAL DESCRIPTION
The Citadel Tissue Processor comprises two main sections: the main body, and the
operating head assembly. Control is provided by a hand-held unit containing a keyboard
and a display.

2.1 MAIN BODY


Ten reagent containers and two wax baths are suspended by their rims from the deck at
the top of the main body. An electrical tray slides into the base of the main body, which
also houses the operating head drive motor, together with associated transformer and
drive electronics.

Wax Baths

Each wax bath is heated independently. The temperature is variable between 45C and
65C, adjustment being made by turning the recessed screw located in the lower half of the
narrow face of the wax bath. A resettable thermal cut-out interrupts the power supply if the
temperature rises to 80C.

Green and amber indicators are provided. The green indicator is lit when power is
applied to the bath and is extinguished if power is interrupted. The amber indicator
illuminates when the heater is energised. The wax baths are plugged into shuttered
mains power outlets on the electrical tray (see below).

Electrical Tray

An electrical tray is located at the base of the main body, and incorporates the following
items:

i Power switch, two mains fuses and a mains inlet cord.

ii Mains power outlets for the wax baths.

iii The connection to the hand-held controller.

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iv Green and amber indicators. The green indicator is lit continuously while the
machine is switched on. The amber indicator is lit in Auto mode to confirm that
processing is in progress, and flashes when an alarm condition is detected. In the
latter case, an audible alarm on the electrical tray is sounded.

v A vacuum unit control link (supplied with the Vacuum Unit).

2.2 OPERATING HEAD ASSEMBLY


The operating head assembly consists of a lift arm assembly enclosed by an operating
head cover. The lift arm assembly raises and lowers the tissue basket into and out of
the reagent. Access to the basket is by way of a door, in the operating head cover,
which is secured by a catch. To provide optimum agitation, the tissue basket is raised
clear of the reagent every ten minutes and then lowered again.

2.3 HAND-HELD CONTROLLER


The controller consists of a keypad and a liquid crystal display, and is connected to the
electrical tray by means of a coiled cable. The keypad controls programming, the mode
of operation, and other functions; and the display provides program and other tissue
processor status information.

The processor may be located in a fume cupboard or safety cabinet, and the hand-held
controller placed outside the cabinet

2.4 OPTIONAL ACCESSORIES


2.4.1 Vacuum Unit

You can apply a vacuum to the wax bath located at position 12. The vacuum unit is
connected to, and controlled from, the mains tray, but is only applied when selected
from the hand-held controller.

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2.4.2 Baskets

Additional random tissue baskets and organiser tissue baskets are available.

Key:

1 - Operating Head Assembly 8 - Item no longer available


2 - Basket Hanger 9 - Hand Held Controller
3 - Evaporation Covers 10 - Electrical Tray
4 - Connection for Vacuum Tube 11 - Auto Mode Indicator
5 - Wax Baths 12 - Power On Indicator
6 - Random Basket 13 - Reagent Containers
7 - Vacuum Unit 14 - Organiser Baskets

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INSTALLATION
The Citadel is a bench-mounted instrument which must be unpacked and installed with
care. Ensure that the bench area is level, made of a non-flammable material, is large
enough to take the instrument (which is 680mm in diameter), and is capable of taking
the instruments weight:

64kg for the Citadel 1000 (52kg dry weight plus 12kg of reagents)

72kg for the Citadel 2000 (48kg dry weight plus 24kg of reagents)

Ensure that any unnecessary heating and cooling is avoided, as this could affect the
evaporation of the reagents, or the temperature control of the wax baths.

Remove the top cover of the packing case and accessory packaging from the top of the
instrument.

THE INSTRUMENT IS HEAVY, SO GET HELP TO LIFT OR MOVE IT, OR USE


APPROPRIATE MECHANICAL HANDLING AIDS. WHEN MOVING OR LIFTING
THE INSTRUMENT, GRIP IT FIRMLY ANYWHERE UNDERNEATH THE PLINTH.

Remove the instrument from the base of the packaging and place it carefully on
the bench. Remove any packing from the operating head cover and check that the
movement of the operating head is not obstructed. Check that the instrument is
undamaged and complete. Contact your Thermo dealer if necessary.

Remove the basket hanger from the packing and fit it into the operating head by
inserting the metal clip on the top of the lid into the corresponding connector on the
head. Push the clip in until the basket hanger is fully home. Close the operating head
cover.

The Citadel is supplied with a moulded mains connector. If this does not match your
mains socket, remove the plug and rewire it as follows:

12 69810040 Issue 6
Brown wire Live (L or L2) terminal

Blue wire Neutral (N or L1) terminal

Yellow/green wire Earth (E or Ground) terminal

ONLY A COMPETENT ELECTRICIAN MAY REWIRE THE VACUUM UNIT PLUG.

Ensure that the mains voltage rating corresponds with the voltage rating on the
specification plate of the instrument (situated at the rear of the base of the instrument).

The ~ symbol on the rating plate indicates that the instrument operates on an
alternating current (ac) supply. If possible, use a power supply with an emergency
generator back-up.

Notes:

1 The Citadel is Class I equipment as defined in IEC1010, and as such it must be


protectively earthed. Ensure that the instrument is properly connected to a good
earth/ground contact, marked:

2 Make sure that it is possible to interrupt the power at a place away from the
instrument. In an emergency, remove the plug from the mains supply socket. Do
not use the instrument ON/OFF switch to disconnect the instrument.

Press O on the ON/OFF (I/O) switch on the electrical tray to ensure that the instrument
is switched off. Plug the mains cable into the power supply.

Press I on the ON/OFF (I/O) switch to switch the instrument on. Check that the POWER
indicator is lit and that the hand-held controller display is displaying STEP 1, PROG A,
B, C, or D and MANUAL.

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To insert and remove the reagent containers

Note:

1 Position 1 is adjacent to the hand-held control unit mounting position. Container


positions are numbered clockwise.

Press the CHECK/FILL key on the hand-held unit. Each time this key is pressed, the
operating head moves to position 2, 6 and 10, to allow access to all the containers.
Remove the evaporation covers, and insert a reagent container into each space. Ensure
that the handle of the container lies flat, to avoid fouling as the operating head rotates.
Note that wax baths must be fitted into positions 11 and 12, which are situated to the
right of the hand-held unit, above the wax bath power connections on the electrical tray.

Press the CHECK/FILL key again and repeat the procedure until there are containers in
all 12 positions. Ensure that the evaporation covers are replaced.

The containers can be removed from the instrument in the same way.

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CONTROLS AND INDICATIONS
The hand-held controller has been designed for easy use. The functions of the touch-
sensitive keys and the display indications are explained in the following tables.

4.1 HAND-HELD CONTROLLER KEYS

Legend Function

Resets the display step number to 1.


RESET

Increases the display step number from 1 to 12 to 1 etc. Press once


to increment the step number by 1. Press and hold to increase the
STEP
step number quickly.

Decreases the display time. Press once to decrease the time by 1


_
minute. Press and hold to decrease the time quickly. Wraps round
from 00.00 to PASS to 99.59.

Increases the display time. Press once to increase the time by 1


minute. Press and hold to increase the time quickly. Wraps around
+ from 99.59 to 00.00.

Changes the displayed program in the sequence A-B-C-D-A-B


etc., where A is the first program and D is the fourth. Press once
PROG to change the program by one step. Press and hold to change the
program quickly.

(Continued)

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Legend Function

Selects the vacuum. VACUUM is displayed. Press once to enable


the vacuum and press again to disable the vacuum. Vacuum is
applied when the operating head is at position 12 and the tissue
VAC basket is lowered, when the processor is in Auto mode. Vacuum is
independently selectable for each program and will be retained by
each program unless cancelled.

Increases the rate of change of display time to 20 per second


when pressed together with + (Plus) or - (Minus).
RAPID

Determines the mode of operation. When switched from Auto


to Manual, any movements which are already in progress are
AUTO MANUAL completed. AUTO or MANUAL is displayed as appropriate.
START STOP

In Manual mode, all the keys are active and a keypress is


indicated by a short audible tone. The ENTER tone is slightly
longer indicating that the time has been stored in the program
displayed.

In Auto mode, only the AUTO/MANUAL, EMERGENCY STOP and


Timing keys are active. In addition, CANCEL ALARM is active if
an alarm condition exists.

For further Details refer to Mode Descriptions (Section 5,


Operating Conditions).

Writes the displayed time into the currently displayed step of the
ENTER current program.

When pressed and held down, displays real time. You can alter the
setting by pressing the + (Plus) or - (Minus) key with the CLOCK
CLOCK key held down.

(Continued)

16 69810040 Issue 6
Legend Function

When pressed and held down, displays the start time of the
START program. You can alter the setting by pressing the + (Plus) or -
TIME (Minus) key with the START TIME key held down.

If the indicator on the key is lit, a delay has been programmed. In


this case, when the DAYS DELAY key is pressed and held down,
DAYS the number of days delay, in 24-hour periods, is displayed. The
DELAY setting can be altered by pressing the + (Plus) or - (Minus) key
with DAYS DELAY held down.

A programmed delay is activated when the key is pressed and is


DELAY de-activated when the key is pressed a second time. When the
ON delay is activated, the indicator on the key is lit.

When the tissue basket is raised, each press causes the head
to advance one position. The switch has no effect if the basket is
ROTATE lowered.

When the tissue basket is lowered, press once to raise the tissue
basket. The key has no effect if the basket is already raised.
RAISE

LOWER When the tissue basket is raised, press once to lower the tissue
basket. The key has no effect if the basket is already lowered.

When the tissue basket is raised and the head is not at position 1,
press once to rotate the head to position 1. Note that position 1 is
GO TO 1 directly above the connection point for the hand-held controller.

(Continued)

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RESET STEP _ +

PROG VAC RAPID

AUTO MANUAL
START STOP ENTER


START DAYS DELAY
CLOCK TIME DELAY ON

LOWER

ROTATE RAISE

GO TO 1 CHECK/FILL

CANCEL
EMERGENCY STOP ALARM

18 69810040 Issue 6
Legend Function

When the tissue basket is raised, press to rotate the head to


positions 2, 6,10, 2, 6 etc. Positioning the head at any of these
CHECK/FILL positions facilitates access to two groups of three reagent vessels
and wax baths via the anti-evaporation covers.

Stops the machine immediately. A continuous beep audible alarm


sounds and the AMBER PROCESSING indicator flashes. If the
EMERGENCY STOP
machine is operating in Auto mode, it reverts to Manual mode.

Clears the alarm condition, providing that the cause of the alarm
has been removed. Press once to silence the alarm then press
again after a 3-second delay to restore the machine to its selected
CANCEL operating mode and program step. If the alarm is caused by
ALARM operator interference or a mechanical obstruction of the head,
then, when you press the key the second time, the head moves
to the nearest position and the tissue carrier lowers and raises. To
resume operation, press the AUTO/MANUAL key.

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4.2 HAND-HELD CONTROLLER DISPLAY

Indication Function

PROG A-B-C-D Indicates which program is selected.

VACUUM Indicates that vacuum is selected for the displayed program.


The indicator flashes while the vacuum is applied.

AUTO/MANUAL Indicates the mode in which the machine is operating.

Digits 1 and 2 Display the current program step in AUTO and MANUAL.

Digits 3, 4, 5, and 6 Display the time stored for the selected step. The time is
counted down to 00:00 in Auto mode. Digits 3 and 4 display
hours, and digits 5 and 6 display minutes.

In Manual mode, the colon is steady.

In Auto mode, the colon flashes when the time is being


counted.

20 69810040 Issue 6
OPERATING CONDITIONS
The two main operating conditions which are set by the operator are:

i Auto mode

ii Manual mode

A further condition, entered automatically is:

Alarm This is automatically activated when the processor detects an alarm condition.

5.1 AUTO MODE


Auto mode is the processing mode of the machine. The tissue processor is
programmed in Manual mode (see below) after which Auto mode is entered by pressing
the AUTO START/MANUAL STOP key.

In this mode, only the AUTO START/MANUAL STOP, EMERGENCY STOP and Timing
keys are active. You can re-enter Manual mode at any point in the program by pressing
AUTO START/MANUAL STOP. If this key is pressed whilst the head or basket carrier is
moving, the movement is completed before Manual mode is entered.

If the EMERGENCY STOP key is pressed while the basket carrier is moving, the
carrier stops immediately, then completes its move when ALARM CANCEL is pressed.
However, if EMERGENCY STOP is pressed while the operating head is rotating,
rotation does not resume when ALARM CANCEL is pressed. In this case, press
ROTATE after ALARM CANCEL to complete the movement.

For details of the automatic program sequence refer to Section 8.2, Program Sequence.

5.2 MANUAL MODE


Manual mode is entered when the tissue processor is first switched on. This mode also
enters automatically when an end of cycle or null run occurs, or when you press the
EMERGENCY STOP key followed by the ALARM CANCEL key. If the processor is in
Auto mode, enter Manual mode by pressing the AUTO START/MANUAL STOP key.

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Once in Manual mode, all the keys are active and a key press is indicated by a short
beep. You can program the processor and execute individual motions (rotate, raise and
lower) of the operating head and basket carrier. Resetting from an emergency stop as
described in Section 5.1, Auto Mode.

5.3 ALARMS
An alarm sounds automatically when an alarm situation occurs, or when you press
the EMERGENCY STOP key. A continuous beep sounds and an alarm code displays,
unless the Alarm mode was entered via the EMERGENCY STOP key, in which case
ES is displayed. Alarms AL 2, 4, 6 and 7 can be caused by obstructions to the head or
basket lift carrier assembly. When these alarms occur, check for any obstruction to the
head or basket lift carrier assembly. If this is not the case, the alarm might be due to
mechanical or electronic wear or failure.

Use the ALARM CANCEL key to cancel the Alarm. Press the key twice, once to cancel
the alarm and a second time, after a 3-second delay, to restore the processor to the
previously selected operating mode and program step. If the cause of the alarm is not
apparent, and pressing ALARM CANCEL twice does not clear the alarm, call your local
Thermo service engineer.

Note that if the alarm was caused by operator interference or a mechanical obstruction
of the head, pressing ALARM CANCEL the second time causes the operating head to
move to the nearest position and the tissue carrier to lower and then raise. You must
then press the AUTO START/MANUAL STOP button to resume operation.

22 69810040 Issue 6
PREPARATION
6.1 GENERAL
Once the processor has been installed, (refer to Section 3, Installation), the containers
can be filled as appropriate. A list of the approved reagents etc. is supplied below,
followed by the filling procedure.

As cooled wax can take up to five hours to remelt, the usual procedure is to switch on
the processor power supply and connect the wax baths already filled with molten wax.
This also ensures that you can detect immediately any malfunction of the wax baths.

6.2 REAGENTS
The following reagents are approved by Thermo for use with the Citadel processor.
Contact your local Thermo dealer for advice before using any reagents that are not
listed below.

i Fixatives: Formalin
Formol Saline
Buffered Formalin
Bouins Fluid

ii Dehydrants: Methanol (Methyl Alcohol)


Ethanol (Ethyl Alcohol)
Isopropyl Alcohol
Acetone

iii Clearing Agents: Xylene


Toluene
Chloroform
Xylene Substitute

iv Paraffin Wax: Histoplast


You may use any good quality paraffin wax

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6.3 FILLING THE CONTAINERS

Before filling the containers, refer to the written program table (Section 7.2, Preparing a
Program Table) to ensure that the reagents are allocated appropriately.

6.3.1 Reagents

1. Ensure that Manual mode is selected:

1 2 3 4 5 6

a. Check that MANUAL is lit on the COL

display.

b. If MANUAL is not displayed press AUTO MANUAL


MANUAL STOP. START STOP

2. Prepare to fill:

a. Press the CHECK/FILL key.


CHECK/FILL

b. Check that operating head moves to position 2, 6 or 10. Each of these


positions allows access to a set of containers.

3. Remove the appropriate anti-evaporation cover.

4. Fill the containers with the selected reagents:

Fill the containers to the maximum level marked on the container. If necessary,
you can remove the reagent containers from the instrument to a fume cupboard
or well-ventilated area for filling. Or you can fill the reagent containers with the
containers fitted in the instrument. However, be aware of the following advice and
precautions:

24 69810040 Issue 6
FOR PLASTIC CONTAINERS, THE FILL LINE IS THE TOP OF THE ROUNDED
FEATURES ON THE SIDE OF THE CONTAINER.

FOR NEWER METAL CONTAINERS, THE FILL LINE IS MARKED ON THE INSIDE
OF THE CONTAINER. FOR OLDER METAL CONTAINERS, THAT DO NOT HAVE
THIS INDICATOR, THE CORRECT FILL LEVEL IS 6.5cm FROM THE TOP OF THE
CONTAINER.

PLASTIC CONTAINERS ARE FOR USE WITH FIXATIVES AND DEHYDRANTS.


METAL CONTAINERS ARE FOR USE WITH CLEANING AGENTS. USING
THE WRONG CONTAINER FOR THE REAGENT TYPE CAN DAMAGE THE
CONTAINER, AND CAUSE INSTRUMENT ERROR.

ALWAYS REFER TO THE RELEVANT MSDS WHEN USING REAGENTS.

USE GOOD LABORATORY PRACTICE WHEN FILLING CONTAINERS.

TAKE CARE WHEN CARRYING FULL REAGENT CONTAINERS, AND WHEN


FITTING FULL CONTAINERS IN THE INSTRUMENT.

DO NOT SPILL REAGENT INTO THE INSTRUMENT. NOTE THAT SOLVENT


VAPOURS WILL BE PRESENT WHEN YOU ARE FILLING THE CONTAINERS.

TAKE CARE NOT TO TRAP YOUR FINGERS WHEN REFITTING THE CONTAINERS
IN THE INSTRUMENT.

CHECK REAGENT CONTAINERS REGULARLY TO ENSURE THE FILL LEVEL IS


CORRECT.

Refer to Section 7.2, Preparing a program Table.

5. When you have filled the containers, replace the anti-evaporation covers.

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6.3.2 Wax

YOU MAY USE SEMI-MOLTEN WAX, OR MOLTEN WAX AT A TEMPERATURE


NOT GREATER THAN 60C, TO FILL THE BATH TO THE DESIRED LEVEL, BUT
POURING WAX HOTTER THAN 60C INTO A COLD BATH CAN DAMAGE THE
SENSITIVE THERMOSTAT. FOR THE SAME REASON, DO NOT ALLOW AN
EMPTY BATH TO HEAT UP.

1. Repeat Steps 1-3 of Section 6.3.1, Reagents.

Ensure access to the wax baths is possible.

2. Fill the wax baths:

Citadel 1000:

Fill the wax baths with molten wax to the bottom of the level indicated on the
inside of the wax bath.

Citadel 2000:

Fill the wax baths with molten wax to the bottom of the lower level (1400ml) when
using organiser baskets, or to the bottom of the upper level (1700ml), when using
random baskets.

You can fill the wax baths either off the instrument, or while the baths are fitted to
the instrument.

TAKE CARE WHEN CARRYING AND FILLING THE WAX BATHS.

TAKE CARE NOT TO TRAP YOUR FINGERS WHEN FITTING THE WAX BATHS.

3. Connect the wax bath cables:

Plug the wax bath cables into the sockets located on the electrical tray at the base
of the processor.

26 69810040 Issue 6
4. Check that the wax baths are on:

Check that the indicators on each wax bath are lit.

Note that heating commences as soon as power is switched on.

TAKE CARE WHEN HANDLING THE WAX BATHS. THERE ARE ACCESSIBLE
HOT SURFACES.

ONLY USE WAX IN THE WAX BATHS. IF YOU USE FLAMMABLE LIQUID, YOU
MAY CAUSE A FIRE.

CHECK THE WAX BATH REGULARLY TO ENSURE THE FILL LEVEL IS


CORRECT.

IF YOU USE A VACUUM UNIT, CHECK THAT THE TUBE IS CLEAR, AND THE
TOP OF THE CONTAINER IS FREE FROM DEBRIS.

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PROGRAMMING
7.1 GENERAL
The processor can store up to four (A, B, C, D) 12-step programs. It is essential that
you create a written record of each processing cycle, for reference purposes. To this
end, prepare a program table, similar to the examples below.

7.2 PREPARING A PROGRAM TABLE


When preparing a program table, consider the following:

i The minimum immersion time in any position/step is one minute.

ii The maximum immersion time in any position/step is 99 hours 59 minutes. This


allows for extended programs (such as weekend programs).

iii The programmed time indicates the end of an immersion period.

iv The lift arm is raised and lowered automatically every ten minutes to provide
mechanical agitation.

v When transferring from position to position, the basket drains for approximately
one minute. This minute is NOT included in program time.

vi You can delay the programmed schedule up to nine days using the delay feature.
Refer to Section 7.7, Entering a Delay Into a Program.

The steps involved in preparing a program table are summarised below.

Step 1. List the reagents and then allocate them to a specific position/step.

Step 2. Insert any critical immersion times.

Step 3. Complete the table.

Note:

1 It is not essential that all twelve steps be used in each program (refer to Section
7.6, Entering a Pass Facility).

28 69810040 Issue 6
Two sample programs are provided:

Routine overnight processing program

Small biopsies program for daytime processing

Example of Program for Routine Overnight Processing

Position/Step Reagent Immersion Period Period Entered


Hrs Mins Hrs Mins

Position 1 Formalin 2.00 02.00


Position 2 Formalin 2.00 02.00
Position 3 Alcohol 70% 1.00 01.00
Position 4 Alcohol 90% 1.00 01.00
Position 5 Alcohol 100% 1.00 01.00
Position 6 Alcohol 100% 2.00 02.00
Position 7 Alcohol 100% 2.00 02.00
Position 8 Xylene 1.00 01.00
Position 9 Xylene 1.30 01.30
Position 10 Xylene 1.30 01.30
Wax Bath 1 (11) Wax 2.00 02.00
Wax Bath 2 (12) Wax 3.00 03.00

END OF CYCLE

Total processing time = 20hrs


Full program time = 20hrs 11mins, allowing one minute for every change of position

Thermo has not validated the protocol illustrated here, and


takes no responsibility for its use. YOU must always validate
protocols before placing any reliance on them.

69810040 Issue 6
29
Example of Day-time Program for Small Biopsies (Such As Needle
Biopsies)

Note:
1 This processing schedule is appropriate for needle biopsies of 2 mm in diameter
and 10 mm in length.

Position/Step Reagent Immersion Period Period Entered


Hrs Mins Hrs Mins

Position 1 Formal Saline 0.15 00.15


Position 2 Formal Saline 0.15 00.15
Position 3 Alcohol 70% 0.15 00.15
Position 4 Alcohol 90% 0.15 00.15
Position 5 Alcohol 100% 0.15 00.15
Position 6 Alcohol 100% 0.15 00.15
Position 7 Alcohol 100% 0.15 00.15
Position 8 Xylene 0.15 00.15
Position 9 Xylene 0.15 00.15
Position 10 Xylene 0.15 00.15
Wax Bath 1 (11) Wax 0.30 00.30
Wax Bath 2 (12) Wax 0.30 00.30

END OF CYCLE

Total processing time = 3hrs 30mins


Full program time = 3hrs 41mins, allowing one minute for every change of position

Thermo has not validated the protocol illustrated here, and


takes no responsibility for its use. YOU must always validate
protocols before placing any reliance on them.

30 69810040 Issue 6
7.3 ENTERING A PROGRAM
Before you can enter a program into the memory, you must switch on the power supply
(refer to Section 3, Installation), and make sure the movement of the operating head is
not obstructed in any way.

All further operations are controlled via the hand-held controller.

1. Select Manual mode:


1 2 3 4 5 6
a. Check that MANUAL is lit on the COL

display.

b. If MANUAL is not displayed, press


MANUAL STOP. AUTO MANUAL
START STOP

2. Select the appropriate program:

a. Press the PROG key to select the A, B, C or D program


PROG
as appropriate.

b. Check that A, B, C or D is lit on the 1 2 3 4


COL
5 6

display. If A is selected, the display


shows:

3. Return to the start of the program:

a. Press the RESET key.


RESET

b. Check that Step 01 is displayed: 1 2 3 4


COL
5 6

69810040 Issue 6
31
4. Set the required immersion time for Step 01:

a. Using the + (Plus), - (Minus) and RAPID keys, as appropriate, set the
required immersion time for Step 01.

b. Press once to increase the display time by one minute.


+

c. Press once to decrease the display time by one minute.


_

d. Press , and either or


RAPID + _

to alter the displayed time rapidly, until the required immersion time is
displayed.

5. Enter the required immersion time into program memory:

Press the ENTER key.


ENTER

A beep sounds, verifying that the time has been entered into the program
memory.

6. Proceed to Step 02:

a. Press the STEP key.


STEP

1 2 3 4 5 6
COL

b. Check that Step 02 is displayed:

7. Repeat Steps 4 and 5.

32 69810040 Issue 6
8. Enter the rest of the program:

a. Press the STEP key.


STEP

b. Repeat Steps 4 and 5 until you have entered the entire program.

c. Check that 03 to 12 are displayed as each step in the program is selected.

d. Adjust the time for each step as described in Step 4.

7.4 CHECKING AN ENTERED PROGRAM


Follow this procedure when you want to review an entered program.

1. Select Manual mode:


1 2 3 4 5 6

a. Check that MANUAL is lit on the COL

display.

b. If MANUAL is not displayed, press AUTO MANUAL


MANUAL STOP. START STOP

2. Select the appropriate program:

a. Press the PROG key to select the A, B, C or D program, as


PROG
appropriate.

b. Check that A, B, C or D is lit on the 1 2 3 4 5 6

display. If A is selected, the display COL

shows:

3. Return to the start of the program:

a. Press the RESET key.


RESET

69810040 Issue 6
33
b. Check that Step 01 and the 1 2 3 4 5
COL
6

appropriate immersion time are


displayed (for example, 1 hour).

c. Go through the full program, pressing the STEP key to move from
one step to another. STEP

d. Check that the entered times displayed are correct throughout the program
sequence.

For example, at Step 03 of program A, with an immersion time of 12 minutes,


the display would show:

1 2 3 4 5 6
COL

4. Check programs in other memory locations:

a. Press the RESET key.


RESET

b. Proceed as in Steps 2a to 3d of Section 7.4, Checking an Entered Program.

7.5 ALTERING A PROGRAM


Adjustments as small as 1 minute can be made at any program step without affecting
any other steps. These adjustments are often desirable in selected program steps, to
compensate for conditions such as reagent dilution or aging.

34 69810040 Issue 6
1. Select Manual mode:

a. Check that MANUAL is lit on the 1 2 3 4 5


COL
6

display.

b. If MANUAL is not displayed, press


MANUAL STOP. AUTO MANUAL
START STOP

2. Select the appropriate program:

a. Press the PROG key to select the A, B, C or D program as PROG


appropriate.

b. Check that A, B, C or D is lit on the


1 2 3 4 5 6
COL

display. If A is selected, the display


shows:

3. Proceed to the step requiring alteration:

a. Press the STEP key the appropriate number of times to reach the
correct place in the program STEP

b. Check that the appropriate step 1 2 3 4 5 6

number is displayed. For example, COL

if Step 03 is selected, the display


shows:

4. Alter the step time as required:

Press keys or , and if required, until the


+ _ RAPID

desired time is displayed.

69810040 Issue 6
35
5. Enter the altered value in the program memory:

Press the ENTER key.


ENTER

A beep sounds, verifying that the time has been entered into the program
memory.

6. Return to the start of the program:

a. Press the RESET key.


RESET

b. Check that Step 01 is displayed.

Note:
1 To completely change a program, follow Steps 1 to 6 for each step of the
program to be amended.

7.6 ENTERING A PASS FACILITY INTO A PROGRAM


If you want to pass over individual reagent or wax containers without the specimen
baskets entering the reagent containers, perform the following steps:

1. Select Manual mode:

a. Check that MANUAL is lit on the 1 2 3 4 5 6


COL
display.

b. If MANUAL is not displayed, press


MANUAL STOP. AUTO MANUAL
START STOP

2. Select the appropriate program:

a. Press the PROG key to select the A, B, C or D program as


appropriate. PROG

36 69810040 Issue 6
1 2 3 4 5 6
b. Check that A, B, C or D is lit on the COL

display. If A is selected, the display


shows:

3. Proceed to the step in the program that you want to pass over:

a. Press the STEP key until the required step is displayed.


STEP

1 2 3 4 5 6
For example, if the step required is COL

Step 03, the display shows:

b. Press the Minus key , , until 00:00 is displayed:


_

1 2 3 4 5 6
COL

4. Enter the PASS facility:

a. Press the - (Minus) key once more.

1 2 3 4 5 6
COL
b. Check that PASS is displayed
across the time:

5. Enter the PASS instruction into the program memory:

Press the ENTER key:


ENTER

IF YOU ENTER 00:00 FOR ANY STEP, PROCESSING STOPS AT THAT STEP.

69810040 Issue 6
37
7.7 ENTERING A DELAY INTO A PROGRAM
If you want a delay period before processing begins, proceed as follows:

1. Select Manual mode:

a. Check that MANUAL is lit on the 1 2 3 4 5


COL
6

display.

b. If MANUAL is not displayed, press


MANUAL STOP. AUTO MANUAL
START STOP

2. Select the appropriate program:

a. Press the PROG key to select the A, B, C or D Program, as


appropriate. PROG

b. Check that A, B, C or D is lit on the 1 2 3 4 5 6

display. If A is selected, the display COL

shows:

3. Return to the start of the processing schedule:

a. Press the RESET key.


RESET

1 2 3 4 5 6

b. Check that Step 01 is displayed: COL

4. Enter the required start time:

a. Hold down the START TIME key. START


TIME

38 69810040 Issue 6
b. Enter the required start time by pressing the + (Plus) and - (Minus) keys until
the required start time is displayed.

The start time is displayed in 24- 1 2 3 4 5


COL
6

hour format. For example, 2.30pm


would be displayed as:

5. Enter the required delay time (in days):

If no days delay is required, go to Step c.

a. Hold down the DAYS DELAY key. DAYS


DELAY

b. Select the required number of days delay using the + (Plus) or - (Minus)
keys. The display shows the number of days (24-hour periods) delayed, in
the format 1 to 9 preceded by the letters dd. For example, a delay of six
days is displayed as:

1 2 3 4 5 6
COL

c. Press the DELAY ON key to activate the delay. DELAY


ON

Check that the indicator in the key lights up, to show that a delay has been
activated.

If a delay has been programmed in, the indicator in the DAYS DELAY key
also lights up.

To cancel the delay press the DELAY ON key once more.

69810040 Issue 6
39
6. Return the head to position 1:

Press the GO TO 1 key.


GO TO 1

7. Load the tissue basket onto the processor

Refer to Section 8.3, Loading the Tissue Baskets, to Section 8.5, Loading the
Basket Hanger into the Citadel.

8. Lower the basket into the fixative at position 1:

LOWER
Press the LOWER key.

9. Automatic countdown to processing:

When the number of days delay has been counted down to 0, the light on the
DAYS DELAY key goes out. When the days delay has counted down to 0 and the
real time passes the programmed start time, the processor goes into Auto mode
and begins processing at the step shown on the hand- held controller display. The
light on the DELAY ON key goes out when processing begins.

On the day of programming, if you programme the number of days delay before
the required start time (for example, the start time is 18:00 but you programme
the days delay at 16:00), then you must add an extra day. See the following
examples.

Example 1 Required processing start time is 1800 on Sunday

Friday Saturday Sunday Monday


Work Weekend Weekend Work
TIME 1600 1800 1800 1800 > Processing
(programmed) (start time)
DAYS DELAY 2 1 0 0
displayed
DAYS DELAY REQUIRED = 2

40 69810040 Issue 6
Example 2 Required processing start time is 1500 on Monday

Friday Saturday Sunday Monday Tuesday


Work Weekend Weekend Holiday Work
TIME 1600 1500 1500 1500 > Processing
(programmed) (start time)

DAYS DELAY 2 1 0
displayed
DAYS DELAY REQUIRED = 2

IF POWER IS LOST DURING THE DELAY PERIOD, THE PROCESSING START


TIME CANNOT BE GUARANTEED.

10. Check that the real clock time is correct:

a. If you need to change the time, hold down the CLOCK key.
CLOCK

b. Press the + (Plus) and - (Minus) keys until the correct time is displayed. Hold
down the RAPID key at the same time, for faster adjustment.

7.8 OVERRIDING A PROGRAMMED DELAY

Press the AUTO START key. AUTO MANUAL


START STOP

The processing schedule begins immediately at whatever step is displayed on the


hand-held controller.

69810040 Issue 6
41
OPERATING PROCEDURE
8.1 RUNNING A PROGRAM
Once you have selected a program manually, it can be initiated and is run automatically,
as follows:

1. Select Manual mode:


1 2 3 4 5 6

a. Check that MANUAL is lit on the COL

display.

b. If MANUAL is not displayed, press AUTO MANUAL


MANUAL STOP. START STOP

2. Select the appropriate program:

a. Press the PROG key to select the A, B, C or D program as


appropriate. PROG

b. Check that A, B, C or D is lit on the 1 2 3 4 5 6

display. If A is selected, the display


COL

shows:

3. Return to the start of the program:

a. Press the RESET key.


RESET

1 2 3 4 5 6

b. Check Step 01 is displayed: COL

42 69810040 Issue 6
4. Return the operating head to the start position:

Press the GO TO 1 key.


GO TO 1

5. Load the tissue basket carrier:

If you are using an organiser basket, load the most urgent samples in the topmost
section for fast access. Refer to Section 8.5, Loading the Basket Hanger Into the
Citadel.

6. Start the Automatic program:

a. Press the AUTO START key.


AUTO MANUAL
START STOP

1 2 3 4 5 6

b. Check that AUTO is lit on the COL

display:

c. The program continues to run to completion, unless you select MANUAL


during a programat which point the cycle stops.

d. When you reselect AUTO, the cycle recommences from the time and step of
the interruption.

THE UNIT CARRIES OUT WHATEVER ACTION SHOWS ON THE DISPLAY.


ALWAYS ENSURE YOU PRESS THE RESET KEY BEFORE STARTING THE
PROGRAM.

69810040 Issue 6
43
8.2 PROGRAM SEQUENCE
An automatic program performs the following sequence:

i The operating head moves to the position corresponding to the first step in the
program, as displayed on the controller.

ii The amber processing indicator on the electrical tray illuminates.

iii The time selected for the current program step is displayed and counted down
to 00:00. Note that the countdown does not begin until the basket carrier is
completely lowered into the reagent vessel. The colon flashes continuously
during processing.

The time display counts down from the selected time to 00:00. The time displayed
at any time is the time remaining in that step.

iv When the count reaches 00:00, the number of the next step is displayed, with
its selected time. Any step displaying PASS instead of a time is omitted, and the
basket will not be lowered into the reagent.

v The operating head moves to the position corresponding to the next step
displayed, and countdown begins again.

vi Steps iv and v are repeated for each subsequent step in the program, until the
final step in the program has been counted down to 00:00, or the time for the next
step has been set to 00:00.

vii When the program is complete, the processor automatically enters Manual mode
and a series of beeps is sounded.

viii The amber processing indicator is extinguished.

ix Press RAISE on the handset, open the load door, and remove the tissue
baskets.

44 69810040 Issue 6
Note:
1 On completion of the final step (Step viii), the tissues remain immersed in the
reagent until you remove them. It is impossible for the processor to transfer the
tissues from Position 12 (End of Program) back to Position 1.

TAKE CARE, AS THE WAX IS HOT.

SOLVENT FUMES MIGHT BE PRESENT WHEN THE LOAD DOOR IS OPENED.

WHEN YOU USE THE VACUUM, RAISE THE BASKET FROM THE CONTAINER
WITH THE LOAD DOOR CLOSED. A VACUUM IN THE LAST STEP CAN CAUSE
THE WAX BATH TO BE LIFTED WITH THE BASKET. IF THE WAX BATH FALLS,
WAX MIGHT SPILL.

8.3 LOADING THE TISSUE BASKETS

8.3.1 Random Basket

Place tissue cassettes loosely in these baskets.

8.3.2 Organiser Basket

Each large section of the organiser basket holds five layers of five cassettesthat is,
25 cassettes in total.

The small section holds two layers of five cassettesthat is, 10 cassettes in total.

The tissue cassettes must be laid flat in the organiser baskets, as dictated by the
basket shape.

ENSURE THAT YOU LOAD TISSUES CORRECTLY INTO THE CASSETTES,


AND THAT THE LID OF THE CASSETTE IS SECURELY FASTENED. USE GOOD
QUALITY TISSUE CASSETTES, SUCH AS THOSE SUPPLIED BY THERMO.

69810040 Issue 6
45
8.4 LOADING THE BASKETS INTO THE HANGER
8.4.1 Organiser Baskets

Insert an organiser basket at the top of the basket hanger, with the narrow side of the
basket towards the centre of the instrument.

Ensure the organiser basket is in as far as it can go, and that the flanges on the basket
hanger line up with the slots in the horizontal flange on the basket. Allow the basket to
slide down to the bottom of the head.

Repeat this with the other baskets.

Capacities:

Citadel 1000 2 x 25 capacity basket


1 x 10 capacity basket
Citadel 2000 4 x 25 capacity basket
1 x 10 capacity basket

Finally, place the organiser lid on top of the baskets.

8.4.2 Random Basket

Fit the basket in the same way as the organiser baskets.

8.5 LOADING THE BASKET HANGER INTO THE CITADEL


Fit the connector on top of the hanger into the slot in the lift arm, and slide the
connector fully home.

REAGENT FUMES ARE PRESENT WHEN YOU OPEN THE LOAD DOOR.

TAKE CARE NOT TO TRAP YOUR FINGERS IN THE LOAD DOOR.

46 69810040 Issue 6
MAINTENANCE
If hazardous material is split on, or inside, the instrument, carry out the appropriate
decontamination. Cleaning or decontamination methods, other than those
recommended in the Operator Guide, must be checked with a Thermo agent to ensure
that the proposed method will not damage the equipment.

9.1 CLEANING AND MACHINE CARE


Always wipe up spills immediately. In the event of a major spillage, disconnect the
machine from the mains supply IMMEDIATELY and do not reconnect it until the
machine has been dried out thoroughly and checked by a service engineer.

Each of the main items, and their recommended cleaning method, are presented in the
table on page 48.

DURING ALL CLEANING PROCEDURES, THE EQUIPMENT MUST BE


DISCONNECTED FROM THE MAINS SUPPLY.

69810040 Issue 6
47
Item Recommended Cleaning Method

Reagent containers Hot water and detergent (maximum temperature 60C)

Wax baths 1. Pour the hot wax into a suitable container.

2. Allow the remaining wax to cool slightly and then


peel away the wax while it is still warm.

Baseplate and top 1. Remove the excess wax using a flexible,


deck non-metallic spatula or a dry cloth.

2. Remove stains using water and detergent.

Central housing Wipe using a moist cloth and detergent.

Baskets and basket 1. Dissolve wax using a suitable solvent.


carrier
2. Wash the basket and basket carrier in hot soapy
water (60-70C) using a mild detergent.

Note: If necessary, you can melt the wax in an oven


heated to 60C.

Operating head 1. Remove wax using a flexible, non-metallic spatula.


assembly and anti-
evaporation covers 2. If necessary, wipe using a moist cloth and
detergent.

Cleaning the instrument regularly, in accordance with the instructions contained within
this Operator Guide, will ensure that the instrument continues to operate efficiently
and safely in normal day-to-day use. Contact your Thermo agent if any part of the
instrument appears to be damaged in any way.

Conduct portable appliance testing (PAT) annually.

48 69810040 Issue 6
9.2 WAX BATH TEMPERATURE

The temperature at which the wax bath operates has been factory set to 60C. Check
the wax bath temperature regularly and, if necessary, adjust it by removing the
protective plastic cap, and turning the recessed screws located in the lower half of the
narrow face of the wax bath.

With the narrow face of the wax bath facing to the front;

Turn anti-clockwise to increase the control temperature.

Turn clockwise to decrease the control temperature.

9.3 DISPOSAL

DISPOSE OF EXHAUSTED REAGENTS IN ACCORDANCE WITH LOCAL


PROCEDURES.

69810040 Issue 6
49
9.4 DECONTAMINATION

If hazardous material is spilt on, or inside, the instrument, YOU


MUST carry out the appropriate decontamination.

Cleaning or decontamination methods, other than those


recommended in the Operator Guide, must be checked with a
Thermo agent to ensure that the proposed method will not
damage the equipment.

Always wear protective gloves when you clean or decontaminate the Citadel,
WARNING to protect yourself against the effects of chemicals.

Do not use any chemicals that might interact with materials of manufacture.
WARNING If in doubt, check with the Thermo Service department.

Phenol and Hypochlorites in strong solution will damage the instrument and
WARNING
its accessories.

Do not use abrasive compounds or metal components to clean the Citadel


WARNING
or its components and accessories.

Always wipe up any spills immediately. In the event of a major


spillage, disconnect the instrument from the mains supply
without delay and do not reconnect and switch on until the
instrument has been thoroughly dried out and checked by a
service engineer.

Refer to the material safety data sheets when handling


reagents.

50 69810040 Issue 6
PRODUCT RETURN SAFETY DECLARATION
Part 1 DECONTAMINATION CERTIFICATE

Any instrument or part of any instrument must be clean before being returned, and where necessary
accompanied by a completed Decontamination Certificate. Should the instrument or any part of it be
received in an unclean condition, or Thermo Fisher Scientific consider it to be a hazard, the instrument or
part will be returned unrepaired at the expense of the customer.
It is important that the certificate is forwarded by post or fax, and a copy attached to the exterior of the
container. Containers will not be opened until the company is in possession of the required certificate.
This form MUST be completed by the customer and NOT a Thermo or distributor employee.
If an instrument or part is to be returned to THERMO, please note the following:-
1 If the instrument or any part of it has been exposed to, or been in contact with potential pathogenic or
radioactive material, it is essential that it is decontaminated.
2 Set procedures are laid down in the European Health and Safety Directives for decontamination. To avoid
any misunderstanding, we request that all instruments or parts returned to us must be accompanied by a
certificate stating the following:
We certify that this (Model) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Serial No
has not been exposed to pathogenic, radioactive or other hazardous material and has been cleaned
OR
has been decontaminated and cleaned (if exposed to the above) according to approved procedures,
following exposure to:

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Signed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Position . . . . . . . . . . . . . . . . . . . . . . . . . .
Name (Block Capitals) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Company or Organisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Full address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Part 2 Guidelines for Returning Instruments

Please use the checklist below to ensure that the instrument being returned is ready for collection.
All reagents / wax removed from instrument, including vapour traps (if applicable). . . . . . . . . . . . . . . . . .

Accessories are secured / itemised. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Instrument has had transit clamps fitted as per operator guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Instrument is packed in original packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . YES / NO

RMA NUMBER. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

CARRIER. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

FOR ATTENTION OF. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Thermo Fisher Scientific, Tudor Road, Manor Park, Runcorn, WA7 1TA, United Kingdom
Tel: +44 (0) 1928 534050; Fax: +44 (0) 1928 534049; www.thermoscientific.com/pathology
69810040 Issue 6
51
TECHNICAL DATA
Citadel 1000 Citadel 2000

Supply voltage range 110-120Vac or 220-240Vac


Maximum supply voltage
fluctuations not to exceed 10%
of the nominal voltage
Supply frequency 50Hz/60Hz (220-240Vac)
50Hz/60Hz (110-120Vac)
Power consumption 500VA
Wax bath heater rating 80W (max)
Wax bath temperature 45-65C

Reagent container capacity 1000ml 2100ml


Wax bath capacity 1000ml 1700ml
Basket cassette capacity - organiser 60 110
- random Approx. 40 Approx. 70
Weight 52kg 48kg

Maximum immersion time per step 99hr 59mins


Minimum immersion time per step 1min
Agitation interval 10mins
Interval between steps 1min
Maximum programmed delay time 9 days
Vacuum pressure range -0.2 to -0.4 bar

Mains Plug Fuse 5A 250V Approved to BS1362


(where applicable)

Instrument Fuse Sizes Thermo


part number
110V -120V
F1 F5A P11171
F2 T1.6A P11131
220V - 240V
F1 F3.15A P11172
F2 T0.8A P11130
Transformer Fuses
2 x T3.15A P05847
Fuses must only be replaced by
technically competent personnel.

52 69810040 Issue 6
10.1 ENVIRONMENTAL CONDITIONS
General Indoor use only

Temperature (operation) +5C to +40C


Temperature (transit/storage) -25C to +55C (+70C for short exposure)

Humidity 80% max for temperatures < 31C


50% max for temperatures > 31C
but < 40C (non-condensing environment)

Altitude up to 2000m

Pollution degree 2
Installation category II

69810040 Issue 6
53
ACCESSORIES
11.1 CITADEL 1000
Description Catalogue No.

1-Litre Carousel Tissue Processor:


UK English version 69800001
German version 69800002
USA version 69800003
European English version 69800008

Accessories sold as separate items

1 Random Basket 69810034

1 Organiser Basket (includes 2 x 25 69810029


baskets and 1 x 10 basket)

1 Basket Lid 69810031

1 Wax Bath (ratings as appropriate)


220-240V 69810050
110-120V 69810052

1 Reagent Container (plastic) 69810019

1 Reagent Container (stainless steel) 69810130

1 Vacuum Unit (ratings as appropriate)


220-240V (UK plug) 69810024
220-240V (European plug) A69810054
110-120 (USA) 69810025

Hyperclean 2 Workstation with Blower Unit (ratings as appropriate)


220-240V 64850602
110-120V 99900602

1 English User Manual 69810040


1 German User Manual 69810040DE
1 French User Manual 69810040FR

To order any of these items, contact your local Thermo dealer.

54 69810040 Issue 6
11.2 CITADEL 2000

Description Catalogue No.

2-Litre Carousel Tissue Processor:


UK English version 69800004
German version 69800005
USA version 69800006
European English Version 69800007

Accessories sold as separate items

1 Random Basket 69810035

1 Organiser Basket (includes 4 x 25 69810030


baskets and 1 x 10 basket)

1 Basket Lid 69810031

1 Wax Bath (ratings as appropriate)


220-240V 69810051
110-120V 69810053

1 Reagent Container (plastic) 69810018

1 Reagent Container (stainless steel) 69810131

1 Vacuum Unit (ratings as appropriate)


220-240V (UK plug) 69810024
220-240V (European plug) A69810054
110-120 (USA) 69810025

Hyperclean 2 Workstation with Blower Unit (ratings as appropriate)


220-240V 64850602
110-120V 99900602

1 English User Manual 69810040


1 German User Manual 69810040DE
1 French User Manual 69810040FR

To order any of these items, contact your local Thermo dealer.

69810040 Issue 6
55
WARRANTY STATEMENT

We are proud of our quality and reliability, and of our after-sales service. We
continuously strive to improve our service to our customers.

Please ask your distributor or representative about Service Contracts which can keep
your purchase in peak condition for many years to come.

Warranty provisions necessarily vary to comply with differences in national and regional
legislation, and you can find details in your delivery documents or from your dealer or
representative.

Please note that your warranty may be invalidated if:


- the instrument is modified in any way,
- accessories and reagents are used that are not approved by Thermo, or
- the instrument is not operated or maintained in accordance with the
instructions in this Operator Guide.

56 69810040 Issue 6
Declaration of Conformity
This Declaration of Conformity, issued under our sole responsibility, is only valid when the instrument
is used in accordance with the instructions for use.

Manufacturers Name: Thermo Shandon Limited (Trading as Thermo Fisher Scientific)

Manufacturers Address: Tudor Road, Manor Park, Runcorn,


Cheshire, WA7 1TA
UNITED KINGDOM

Product Description: Tissue Processor

Product Designation: Citadel


Part numbers: 69800001 69800008 (inclusive
including accessories supplied as standard

Year of Marking (CE): 1995

This product conforms to the essential requirements of the following directives:

In Vitro Diagnostics Directive 98/79/EC

Machinery Directive 2006/42/EC

This product complies with the following International Standards:

EMC: EN 61326
EN 61000-3-2
EN 61000-3-3

Safety: IEC 61010-2-010

Issued by: K. Waldron


Quality Manager
Thermo Fisher Scientific
Anatomical Pathology Division

Date: 20 April 2010

Optional accessories considered subject to the In Vitro Diagnostic Medical Devices Directive (IVDD) are specifically identified on this Declaration of
Conformity. Further supplies of standard accessories are treated as spares. Convenience aids offered as accessories are not subject to the IVDD.

Anatomical Pathology Division Tudor Road Cheshire +44 (0) 1928 534000 www.thermo.com
Manor Park WA7 1TA +44 (0) 1928 534001 fax
Runcorn UK

Thermo Shandon Limited


Registered Office: 19 Mercers Row, Cambridge CB5 8BZ, UK. Registered in England and Wales No. 330973

69810040 Issue 6
57
INDEX

A
Altering a program 34 F
Automatic countdown to processing 40
Automatic program 43 Fuse sizes 52

B H
Baskets 11 Hand-held controller 7, 9, 10, 15
Cleaning 48 Display 20
Organiser 7 Illustration 18
Capacities 46, 52
Loading 45 I
Loading into hanger 46
Immersion times 28, 32, 34, 52
Random 7
Indicators
Capacities 52
Amber 9, 10
Loading 45
Green 9, 10
Loading into hanger 46
INSTALLATION 12
Tissue 17, 19, 43
Basket hanger 12
K
Cleaning 48
Loading into Citadel 46 Keys
ALARM CANCEL 22
C AUTO/MANUAL STOP 16
CANCEL ALARM 19
Checking an entered program 33
CHECK/FILL 14, 19
Cleaning and machine care 47
CLOCK 16
Cleaning methods 48
DAYS DELAY 17
Connecting wax bath cables 26
DELAY ON 17
CONTROLS AND INDICATIONS 15
EMERGENCY STOP 19, 21
ENTER 16
D GO TO 1 17
DECLARATION OF CONFORMITY 57 LOWER 17
Decontamination 47, 50 MINUS 15
Disposal of reagents 49 PLUS 15
PROG 15
E RAISE 17, 44
RAPID 16
Electrical tray 9 RESET 15
Amber indicator 44 ROTATE 17
Entering a delay into a program 38 START TIME 17
Entering a PASS facility 36 STEP 15
ENTERING A PROGRAM 31 VAC 16
Environmental conditions 53
Evaporation covers 14, 25
Cleaning 48

58 69810040 Issue 6
L Reviewing 33
Running 42
Lift arm assembly 10 Program delays 38
Loading baskets into the hanger 46 Program sequence 44
Loading the hanger into the instrument 46 Program tables 28
Loading the tissue baskets 45 Examples 2930
Preparing 28
M
R
MAINTENANCE 47
Material Safety Data Sheets (MSDSs) 2, 25, Reagents 23
50 Clearing agents 23
Mode of operation Dehydrants 23
Manual 20 Disposal 49
Fixatives 23
O Paraffin wax 23
Reagent containers 23
ON/OFF switch 13 Cleaning 48
OPERATING CONDITIONS 21 Filling 24
Operating head assembly 9, 10, 14, 31, 44 Fill levels 24
Cleaning 48 Inserting or removing 14
Operating mode 20 Illustration 14
Alarm 21, 22 Passing over 36
Auto 16, 20, 21 Rewiring the plug 12
Manual 16, 21 Running a program 42
OPTIONAL ACCESSORIES 10
Vacuum unit 10 S
Overriding program delays 41
SAFETY FEATURES 8
P Safety precautions 5
Safety testing
Parts list PAT 48
Citadel 1000 54 Symbols and Conventions 2
Separate accessories 54
Citadel 2000 55 T
Separate accessories 55
PAT testing 48 TECHNICAL DATA 52
Plug Tissue processor
Fuses 52 Cassettes 7
Rewiring 12 Electrical tray 9
PREPARATION 23 Hand-held controller 7
Product Return Safety Declaration 51 Illustration 11
Programmed delays 17 Introduction 7
Examples 4041 Main body 9
Overriding 41 Operating head assembly 9
PROGRAMMING 28 Size and weight 12, 52
Programs (processing) 7 Illustration 53
Altering 34 Unpacking 12
Automatic 43 Wax baths 9
Sequence 44 Tissue samples 7
Automatic countdown 40
Delays 38
Entering 31
Introduction 28
Passing over a container 36

69810040 Issue 6
59
U
Unpacking 12

V
Vacuum unit 7, 10, 16, 20
Voltages 5, 13, 52

W
Warranty Statement 56
Wax baths 9, 23
Cleaning 48
Connecting cables to 26
Filling 26
Fill levels 26
Indicators 27
Temperature 49
Wax baths temperature 49
Increasing or decreasing 49

60 69810040 Issue 6