winCATS Planar Chromatography Manager

Analysis Report
SOP document
Validated Design
Description :

Analysis C:\CAMAG\winCATS\Data\Zolla levodopa 18 juli presisi

interday6 totol u
tk 5,6,7.cna

Created/used by Administrator 18 Juli 2017 17:56:36

Current user Administrator

Stationary phase
Executed by Administrator 07 Juni 2017 8:38:12
Plate size (X x Y) 7.0 x 7.0 cm
Material HPTLC plates silica gel 60 F 254
Manufacturer E. MERCK KGaA
Batch
GLP code
Pre-washing No
Modification No

Definitions - Quantification
Executed by Administrator 12 Juni 2017 9:26:02

Calibration parameters
Calibration mode Multi level
Statistics mode CV
Evaluation mode Peak area

Samples
Sample ID: Standar 1
Sample ID: Standar 2
Sample ID: Standar 3
Sample ID: Standar 4
Sample ID: Standar 5
Window Batch Product
Substance name Rf size Regression Deviation Purity Manufacturer number Expiry date number

Benserazid 0.09 0.5 mm Linear 10.0 % 1.0000

Standards absolute
Standard level1
Substance Amount/fraction
Benserazid 350.0000 mg

Standard level2
Substance Amount/fraction
Benserazid 350.0000 mg

Standard level3
Substance Amount/fraction
Benserazid 350.0000 mg

Standard level4
Substance Amount/fraction
Benserazid 350.0000 mg

Standard level5
Substance Amount/fraction
Benserazid 350.0000 mg

User : Administrator Approved :. . . . . . . . . . . . . . . . . . . SN 1811W027, V1.4.7

18 Juli 2017 17:56:43 Report ID : 07E1071203113824 Page 1 of 6
 winCATS Planar Chromatography Manager

Sample application - Nanomat / Manual device

Instrument: Nanomat / Capillary Dispenser System
Executed by: Administrator 19 Mei 2017 13:25:49

Manual application parameters

Application mode: Contact
Number of tracks: 5
First application position X: 10.0 mm
Application position Y: 10.0 mm
Distance between tracks: 10.0 mm

Sequence

No. Appl. position Appl. volume Vial # Sample ID Active

1 10.0 mm 5.000 l 1 Standar 1 Yes
2 20.0 mm 5.000 l 2 Standar 2 Yes
3 30.0 mm 5.000 l 3 Standar 3 Yes
4 40.0 mm 5.000 l 4 Standar 4 Yes
5 50.0 mm 5.000 l 5 Standar 5 Yes

Development - Glass tank

Chamber type Twin Trough Chamber 20x20cm
Executed by Administrator 18 Mei 2017 13:14:18
Comment
Pre-conditioning
Mobile phase
Solvent front position 100.0 mm
Volume 10.0 ml
Drying device Oven
Temperature 60 C
Time 5 Minutes
Notes

Detection - CAMAG TLC Scanner

Information
Application position 10.0 mm
Solvent front position 60.0 mm

Instrument CAMAG TLC Scanner "Scanner_181122" S/N 181122 (2.01.02)

Executed by Administrator 18 Juli 2017 17:56:36
Number of tracks 6
Position of first track X 10.0 mm
Distance between tracks 10.0 mm
Scan start pos. Y 10.0 mm
Scan end pos. Y 60.0 mm
Slit dimensions 12.00 x 0.90 mm, Macro
Optimize optical system Light
Scanning speed: 20 mm/s
Data resolution: 100 m/step

Measurement Table
Wavelength 280
Lamp D2 & W
Measurement Type Remission
Measurement Mode Absorption
Optical filter Second order
Detector mode Automatic
PM high voltage 289 V

User : Administrator Approved :. . . . . . . . . . . . . . . . . . . SN 1811W027, V1.4.7

18 Juli 2017 17:56:43 Report ID : 07E1071203113824 Page 2 of 6
 winCATS Planar Chromatography Manager

Integration
Properties
Data filtering Savitsky-Golay 7
Baseline correction Lowest Slope
Peak threshold min. slope 5
Peak threshold min. height 10 AU
Peak threshold min. area 50
Peak threshold max. height 990 AU
Track start position 46.2 mm
Track end position 51.2 mm
Display scaling Automatic
All tracks at WavelengthSc4

300

[ AU ]

200

150

100

50 70
[ mm ]
50
0
0.40 40
0.41 30
0.42
0.43 20
[ Rf ] 10
0.45
0

Track 1, ID: Standar 1

300 300

250 250

200 200

150 150

100 100

50 50
1

0 0
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90

Start Start Max Max Max End End Area

Peak Rf Height Rf Height % Rf Height Area % Assigned substance
1 0.43 0.1 0.45 13.4 100.00 0.45 9.2 201.2 100.00 unknown *

User : Administrator Approved :. . . . . . . . . . . . . . . . . . . SN 1811W027, V1.4.7

18 Juli 2017 17:56:43 Report ID : 07E1071203113824 Page 3 of 6
 winCATS Planar Chromatography Manager

Track 2, ID: Standar 2

300 300

250 250

200 200

150 150

100 100

50 50 1

0 0
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90

Start Start Max Max Max End End Area

Peak Rf Height Rf Height % Rf Height Area % Assigned substance
1 0.41 0.1 0.44 25.8 100.00 0.46 1.6 516.9 100.00 unknown *

Track 3, ID: Standar 3

300 300

250 250

200 200

150 150

100 100

1
50 50

0 0
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90

Start Start Max Max Max End End Area

Peak Rf Height Rf Height % Rf Height Area % Assigned substance
1 0.41 0.1 0.44 36.1 100.00 0.46 1.4 802.1 100.00 unknown *

Track 4, ID: Standar 4

300 300

250 250

200 200

150 150

100 100

1
50 50

0 0
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90

Start Start Max Max Max End End Area

Peak Rf Height Rf Height % Rf Height Area % Assigned substance
1 0.40 0.3 0.44 41.0 100.00 0.46 0.8 948.9 100.00 unknown *

User : Administrator Approved :. . . . . . . . . . . . . . . . . . . SN 1811W027, V1.4.7

18 Juli 2017 17:56:43 Report ID : 07E1071203113824 Page 4 of 6
 winCATS Planar Chromatography Manager

Track 5, ID: Standar 5

300 300

250 250

200 200

150 150

100 100

50 50

0 0
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90

Start Start Max Max Max End End Area

Peak Rf Height Rf Height % Rf Height Area % Assigned substance
1 0.40 0.3 0.44 51.1 100.00 0.46 0.6 1172.6 100.00 unknown *

Track 6, ID:
300 300

250 250

200 200

150 150

100 100

50 50

0 0
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90

Start Start Max Max Max End End Area

Peak Rf Height Rf Height % Rf Height Area % Assigned substance
1 0.40 0.8 0.44 46.6 100.00 0.45 0.6 1091.1 100.00 unknown *

Spectrum scan
Executed by Administrator 18 Juli 2017 12:03:54
Mode All detected peaks
Slit dimensions 12.00 x 0.90 mm, Macro
Optimize optical system Resolution
Scanning speed 100 nm/s
Data resolution 10 nm/step
Reference spectrum, pos X 10.0 mm
Reference spectrum, pos Y 10.0 mm

Measurement Table
Lamp D2 & W
Start wavelength 200 nm
End wavelength 700 nm
Measurement type Remission
Measurement Mode Absorption
Optical filter Second order
Detector Mode Automatic

User : Administrator Approved :. . . . . . . . . . . . . . . . . . . SN 1811W027, V1.4.7

18 Juli 2017 17:56:43 Report ID : 07E1071203113824 Page 5 of 6
 winCATS Planar Chromatography Manager

Detector properties
Y-position for 0 adjust 0.0 mm
Track # for 0 adjust 0
Analog Offset 10%
Sensitivity Automatic (33)

Benserazid on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 450.0 500.0 550.0 600.0 [ nm ] 700.0

Evaluation results

Evaluation Sequence
Track Track type
1 Sample
2 Sample
3 Sample
4 Sample
5 Sample
6 Sample
Vial Sample ID
1 Standar 1
2 Standar 2
3 Standar 3
4 Standar 4
5 Standar 5

Table of substances
Position Tracks
Substance MD mm 1 2 3 4 5 6
Benserazid 18.2

Results per track

winCATS summary report

Calibration results per Analysis

No results can be calculated due to the following error(s):
No substances assigned

User : Administrator Approved :. . . . . . . . . . . . . . . . . . . SN 1811W027, V1.4.7

18 Juli 2017 17:56:43 Report ID : 07E1071203113824 Page 6 of 6