Mung bean nuclease

Mung bean nuclease is a nuclease derived from mung Mung Bean Nuclease catalyzes the specic degradation
beans that removes nucleotides in a step-wise manner of single-stranded DNA or RNA, and produces mono-
from single stranded DNA molecules (ssDNA) and is and oligonucleotides carrying a 5-P terminus.
used to remove such ssDNA from a mixture also contain- More than 1000- fold amount of enzyme can degrade
ing double stranded DNA (dsDNA). Mungbean Nuclease
oligomer into all mononucleotides.
is a singlestrand- specic nuclease puried from sprouts
of mung bean Vigna radiata. An excess of the enzyme is required to degrade double-
stranded DNA or RNA and DNA-RNA hybrids, and in
The enzyme degrades single-stranded DNA or RNA this case, AT-rich regions are selectively degraded.
to nucleoside 5-monophosphates, but does not di-
gest double-stranded DNA, double-stranded RNA, or This enzyme work well at ApN, T pN sites, and espe-
DNA / RNA hybrids. Mung Bean Nuclease catalyzes cially ApN sites are 100% degraded.
the specic degradation of single-stranded DNA or RNA, However, it is dicult to degrade CpC, CpG site.
and produces mono and oligonucleotides carrying a 5-
P terminus. Mung bean exonuclease is a nuclease derived from mung
beans that removes nucleotides in a step-wise manner
Molecular Weight: Theoretical: 39 kDa Mung bean nu- from single stranded DNA molecules and is used to re-
clease has a stringent single-stranded specicity for DNA move such ssDNA from a mixture also containing double
or RNA and produces 5-phosphoryl oligo- and mononu- stranded DNA (dsDNA).
cleotides. This enzyme is ideal for transcript mapping,
removal of single-stranded regions in DNA hybrids or Unit Denition:
single-stranded overhangs produced by the restriction en- One unit of Mung Bean Nuclease converts 1 g of heat-
zymes etc. Mung bean nuclease requires Zn2+. The ad- denatured calf thymus DNA into an acid-soluble form in
dition of EDTA or SDS causes irreversible inactivation. 1 minute at 37 C under standard assay conditions.
Do not use at pH below 4.6. Mung bean nuclease should
not be used at low salt concentration.
2 Applications
Removal of hairpin loops during cDNA synthesis.
1 Description
High-resolution mapping of the termini and exon
structures of RNA transcripts (commonly termed
A single-strand specic DNA and RNA endonuclease
Berk-Sharp or S1 Mapping) using either internal-
which will degrade single-stranded extensions from the
labeled or end-labeled probes.
ends of DNA and RNA molecules, leaving blunt, ligat-
able ends. Restriction-site modication or removal by digestion
Mung Bean Nuclease is a single-strand-specic nuclease of single-stranded protruding ends.
puried from sprouts of the mung bean Vigna'''''radiata. Cleavage of single-basepair mismatches, as a re-
Because Mung Bean Nuclease has higher specicity for placement for CEL 1 Nuclease in TILLING.
ssDNA and RNA than S1 Nuclease, it is the enzyme of
choice for most applications requiring a single-strand- Unidirectional deletion of large DNA (in combina-
specic nuclease. tion with Exonuclease III) to generate ordered dele-
tions for sequencing.
Unlike S1 Nuclease, Mung Bean Nuclease will not cleave
the intact strand of nicked duplex DNA. Removal of 3 and 5 extensions from DNA or RNA
termini.
Its ability to recognize double-stranded nucleic acids de-
pends on the base sequence. Transcriptional mapping.
It tends to cleave at ApN and at T(U) pN. It completely Cleavage of hairpin loops.
degrades ApA, but does not degrade G and C. Unlike S1
Nuclease, it does not cleave the strand opposite to that Excision of gene coding sequences from genomic
which has been nicked. DNA.

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3 References
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4 Text and image sources, contributors, and licenses

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