Last updated on: 9/19/2016 8:45:06 AM PST

Vaccine Ingredients and Manufacturer Information

(alphabetical order by vaccine)

We have listed vaccine ingredients (substances that appear in

the final vaccine product), process ingredients (substances used
to create the vaccine that may or may not appear in the final
vaccine product), and growth mediums (the substances
vaccines are grown in) for vaccines approved by the Food &
Drug Administration (FDA) and commonly recommended by
the Centers for Disease Control(CDC.) Controversial products
used to make vaccines: African Green Monkey (Vero)
cells, aluminum, cow products, Cocker Spaniel
cells, formaldehyde, human fetal lung tissue cells, insect
products, and mouse brains.
I. Vaccines and Ingredients
II. Glossary and Details for Ingredients
Though not listed, each vaccine contains strains of the virus
III. Sources
being vaccinated against. Each vaccine entry links to the
manufacturer's package insert that contains information about
dosage, ingredient quantity, and how the vaccine is made. Some
vaccines, like influenza vaccines, are modified frequently and
you may wish to consult the package inserts online and your
doctor for the most current information.

I. VACCINES AND INGREDIENTS

1. Adenovirus 18. Influenza A & B
2. Anthrax 19. Japanese Encephalitis
3. BCG (tuberculosis) 20. Meningococcal
4. Cholera 21. Measles
5. DT (diphtheria & tetanus) 22. MMR (measles, mumps, & rubella)
6. DTap (diphtheria, tetanus, & pertussis) 23. Pneumococcal
7. DTap-IPV (diphtheria, tetanus, pertussis, & polio) 24. Polio
8. DTap-HepB-IPV (diphtheria, tetanus, pertussis, hepatitis B, & polio) 25. Rabies
9. DTap-IPV/Hib (diphtheria, tetanus, pertussis, polio, & haemophilus influenzae type 26. Rotavirus
B) 27. Rubella
10. Hib (haemophilus influenzae type B) 28. Smallpox
11. Hib/Hep B (haemophilus influenzae type B & hepatitis B) 29. TD (tetanus & diphtheria)
 12. Hep A (hepatitis A) 30. Tdap (tetanus, diphtheria, & pertussis)
13. Hep B (hepatitis B) 31. Typhoid
14. Hep A/Hep B (hepatitis A & hepatitis B) 32. Varicella (chickenpox)
15. HPV (human papillomavirus) 33. Yellow Fever
16. Influenza A (H1N1) (swine flu) 34. Zoster (shingles)
17. Influenza A (H5N1) (bird flu)

1. Adenovirus Vaccine
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

sucrose, D-mannose, D-fructose,

dextrose, potassium phosphate,
Barr Labs/Teva plasdone C, anhydrous lactose,
Adenovirus Type 4 and human-diploid fibroblast cell cultures
Pharmaceuticals micro-crystalline cellulose, polacrilin
Type 7 Vaccine, Live, (WI-38), Dulbecco's Modified Eagle's
(under contract with potassium, magnesium stearate,
Oral Medium, fetal bovine serum, sodium
US Army) cellulose acetate phthalate, alcohol,
-- bicarbonate
Apr. 2014 acetone, castor oil, FD&C Yellow
#6 aluminum lake dye, human serum
albumin, monosodium glutamate

2. Anthrax Vaccine
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Anthrax Vaccine Emergent aluminum hydroxide, benzethonium

amino acids, vitamins, inorganic salts
Adsorbed Biosolutions chloride, formaldehyde, proteins
and sugars
Biothrax Nov. 2015 (including 83kDa protective antigen)

3.BCG Vaccine
(tuberculosis)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Organon Teknika glycerin, asparagine, citric acid,

BCG Live
Corp. potassium phosphate, magnesium lactose, saline
Tice BCG
Feb. 2009 sulfate, iron ammonium citrate

PROPER NAME
COMMERCIAL NAME
(click for package insert)
Cholera Vaccine Live
Oral
Vaxchora
PROPER NAME
COMMERCIAL NAME
(click for package insert)
Diphtheria and Tetanus
Toxoids Adsorbed
--
PROPER NAME
COMMERCIAL NAME
(click for package insert)
Diphtheria and Tetanus
Toxoids and Acellular
Pertussis Vaccine
Adsorbed
Daptacel
Diphtheria and Tetanus
Toxoids and Acellular
4. Cholera Vaccine
(tuberculosis)
MANUFACTURER VACCINE INGREDIENTS
GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
ascorbic acid, Hy-Case SF
Pax Vax Bermuda
casamino acids, yeast extract, mineral (hydrolyzed casein), sodium chloride,
Ltd.
salts, anti-foaming agent sucrose, dried lactose, sodium
June 2016
bicarbonate, sodium carbonate
DT Vaccine
5.
(diphtheria & tetanus)
MANUFACTURER VACCINE INGREDIENTS
GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
peptone, bovine extract, modified
Sanofi Pasteur, Ltd. Mueller and Miller medium, aluminum
phosphate, isotonic sodium chloride aluminum phosphate, formaldehyde
June 2013
solution, casein, cysteine, dextrose,
uracil, inorganic salts, vitamins
DTap Vaccine
6.
(diphtheria, tetanus, & pertussis)
MANUFACTURER VACCINE INGREDIENTS
GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
Stainer-Scholte medium, modified
Muellers growth medium, modified
Mueller-Miller casamino acid
Sanofi Pasteur, Ltd. medium (without beef heart infusion), aluminum phosphate, formaldehyde,
2013 casamino acids, dimethyl-beta- glutaraldehyde, 2-Phenoxyethanol
cyclodextrin, ammonium
sulfate, aluminum phosphate,
glutaraldehyde, formaldehyde
GlaxoSmithKline formaldehyde, glutaraldehyde, Fenton aluminum hydroxide, polysorbate 80,
Biologicals medium (containing bovine sodium chloride, formaldehyde
Pertussis Vaccine
Adsorbed
Infanrix
Diphtheria and Tetanus
Toxoids and Acellular
Pertussis Vaccine
Adsorbed
Tripedia*
*This vaccine has been
discontinued.
PROPER NAME
COMMERCIAL NAME
(click for package insert)
Diphtheria and Tetanus
Toxoids and Acellular
Pertussis Adsorbed
and Inactivated
Poliovirus Vaccine
Kinrix
Diphtheria and Tetanus
Toxoids and Acellular
Pertussis Adsorbed
and Inactivated
Poliovirus Vaccine
Quadracel
2013 extract), modified Latham
medium (derived from bovine
casein), modified Stainer-Scholte liquid
medium, aluminum hydroxide
sodium phosphate, peptone-based
medium, bovine extract (US
sourced), formaldehyde, ammonium
thimerosal, gelatin, polysorbate
Sanofi Pasteur, Inc. sulfate, aluminum potassium
80(Tween
Dec. 2005 sulfate, modified Mueller and Miller
80), formaldehyde, aluminum
medium, modified Stainer-Scholte
medium, isotonic sodium, chloride
solution, sodium phosphate
DTap-IPV Vaccine
7.
(diphtheria, tetanus, pertussis, & polio)
MANUFACTURER VACCINE INGREDIENTS
GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
formaldehyde,
glutaraldehyde, aluminum
hydroxide, Vero cells, calf serum,
polysorbate 80 (Tween 80), neomycin
GlaxoSmithKline lactalbumin hydrolysate, Fenton
sulfate, polymyxin B, aluminum
Biologicals medium (containing bovine
hydroxide, sodium
2016 extract), modified Latham
chloride, formaldehyde
medium (derived from bovine
casein), modified Stainer-Scholte liquid
medium
modified Mueller's growth medium,
ammonium sulfate, modified Mueller-
Miller casamino acid medium(without aluminum phosphate, 2-
beef heart infusion), aluminum phenoxyethanol, water, polysorbate
Sanofi Pasteur phosphate, modified Stainer-Scholte 80, formaldehyde,
Mar. 2015 medium (added casamino acids and glutaraldehyde, bovine serum
dimethyl-beta-cyclodextrin), albumin, neomycin, polymyxin B
glutaraldehyde, formaldehyde, MRC-5 sulfate
cells(with added Medium 199 without
calf serum)
 DTap-HepB-IPV Vaccine
8.

(diphtheria, tetanus, pertussis, hepatitis B, & polio)

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Diphtheria and Tetanus formaldehyde, gluteraldehyde, aluminum

hydroxide, aluminum phosphate,
Toxoids and Acellular
lactalbumin hydrolysate, calf
Pertussis Adsorbed, polysorbate 80 (Tween 80), neomycin
GlaxoSmithKline serum, Fenton
Hepatitis B sulfate, polymyxin B, yeast
Biologicals medium(containing bovine
(Recombinant) and protein, aluminum salts, sodium
2016 extract), modified Latham
Inactivated Poliovirus chloride, formaldehyde
Vaccine Combined medium (derived from bovine
casein), modified Stainer-Scholte liquid
Pediarix
medium, Vero cells

DTap-IPV/Hib Vaccine
9.

(diphtheria, tetanus, pertussis, polio, & haemophilus influenzae type B)

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

aluminum phosphate, formaldehyde,

Diphtheria and Tetanus glutaraldehyde, 2-phenoxyethanol,
Toxoids and Acellular modified Muellers Growth
Pertussis Adsorbed, polysorbate 80, bovine serum
Medium, Mueller-Miller casamino acid
albumin, neomycin, sucrose,
Inactivated Poliovirus medium (without beef heart infusion),
Sanofi Pasteur, Inc. glutaraldehyde, polymyxin B
and Haemophilus b modified Stainer-Scholte medium (with
Conjugate (Tetanus 2013 sulfate, aluminum
added casamino acids, dimethyl-beta-
phosphate, formaldehyde, 2-
Toxoid Conjugate) cyclodextrin), MRC-5 cells, CMRL
Vaccine phenoxyethanol
1969 medium (supplemented with calf
Pentacel serum), ammonium sulfate, water,
Medium 199 (without calf serum)

Hib Vaccine
10.

(haemophilus influenzae type B)

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Haemophilus b Sanofi Pasteur, Inc. ammonium sulfate, Modified Mueller sucrose, purified capsular
Conjugate Vaccine May 2016 and Miller medium, saline polysaccharide
(Tetanus Toxoid diluent, formaldehyde, casein derivatives
Conjugate)
ActHIB
Haemophilus b
Conjugate Vaccine GlaxoSmithKline lactose, sodium
formaldehyde, synthetic medium, semi-
(Tetanus Toxoid Biologicals chloride, formaldehyde, purified
synthetic medium, saline diluent, lactose
Conjugate) 2016 capsular polysaccharide
Hiberix
Haemophilus B
Conjugate Vaccine Merck Sharp & amorphous aluminum
(Meningococcal Dohme Corp. complex fermentation media, ethanol hydroxyphosphate sulfate, sodium
Protein Conjugate) Dec. 2010 chloride
Liquid PedvaxHIB

Hib/Hep B Vaccine
11.

(haemophilus influenzae type B & hepatitis B)

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Haemophilus b
Conjugate yeast (vaccine contains no detectable
(Meningococcal yeast DNA), nicotinamide adenine
Protein Conjugate) and dinucleotide, hemin chloride, soy
aluminum hydroxyphosphate
Hepatitis B Merck & Co., Inc. peptone, dextrose, mineral salts, amino
sulphate, sodium
(Recombinant) Dec. 2010 acids, formaldehyde,
chloride, formaldehyde
Vaccine potassium aluminum sulfate,
COMVAX* amorphous aluminum hydroxyphosphate
*This vaccine has been sulfate, sodium borate
discontinued.

12. Hep A Vaccine

(hepatitis A)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

GlaxoSmithKline MRC-5 cells aluminum hydroxide, amino acid

Hepatitis A Vaccine, Biologicals supplement, polysorbate
Inactivated 2016 20, formaldehyde, neomycin sulfate,
Havrix phosphate buffered saline, MRC-5
cellular proteins, aminoglycoside
antibiotic

amorphous aluminumhydroxyphosphate bovine albumin, sodium

Hepatitis A Vaccine, Merck & Co., Inc.
sulfate, formaldehyde, neomycin, MRC- borate, formaldehyde, non-viral
Inactivated Dec. 2010
5 fibroblasts proteins, sodium chloride, neomycin
VAQTA

13. Hep B Vaccine

(hepatitis B)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Hepatitis B Vaccine GlaxoSmithKline aluminum hydroxide, yeast protein,

phosphate buffers, sodium chloride,
(Recombinant) Biologicals aluminum hydroxide
sodium dihydrogen, phosphate
Engerix-B 2016
dihydrate
yeast protein, soy peptone, dextrose,
Hepatitis B Vaccine amino acids, mineral salts, alum
Merck & Co., Inc. formaldehyde, yeast
(Recombinant) (potassium aluminum
2014 protein, aluminum
Recombivax sulfate), amorphous aluminum
hydroxyphosphate sulfate, formaldehyde

Hep A/Hep B Vaccine

14.

(hepatitis A & hepatitis B)

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

neomycin sulfate, MRC-5 cell

Hepatitis A Inactivated proteins, formaldehyde, yeast protein,
GlaxoSmithKline
& Hepatitis B yeast protein, MRC-5 water, aluminum
Biologicals
(Recombinant) Vaccine cells, formaldehyde, aluminum salts phosphate, aluminum hydroxide,
2016
Twinrix amino acids, sodium chloride,
phosphate buffer, polysorbate 20
 HPV Vaccine
15.

(human papillomavirus)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Human Papillomavirus
vitamins, amino acids, lipids, mineral insect cell and viral protein, sodium
Bivalent (Types 16 and GlaxoSmithKline
salts, aluminum (as hydroxide salt), chloride, aluminum hydroxide,
18) Vaccine, Biologicals
sodium dihydrogen phosphate dihydrate, bacterial cell protein, sodium
Recombinant 2016 sodium chloride, water dihydrogen phosphate
Cervarix
Human Papillomavirus
yeast protein, vitamins, amino acids, L-histidine, polysorbate 80, sodium
Quadrivalent (Types 6,
Merck & Co., Inc. mineral salts, carbohydrates, amorphous borate, amorphous aluminum
11, 16, 18) Vaccine,
2015 aluminum hydroxyphosphate hydroxyphosphate sulfate adjuvant,
Recombinant
sulfate, aluminum-containing adjuvant sodium chloride, yeast protein, water
Gardasil
Human Papillomavirus vitamins, amino acids, mineral salts, aluminum (Amorphous Aluminum
9-valent Vaccine, Merck & Co., Inc. carbohydrates, yeast cells, aluminum- Hydroxyphosphate Sulfate, sodium
Recombinant 2015 containing adjuvant (Amorphous chloride, L-histidine, polysorbate 80,
Gardasil 9 Aluminum Hydroxyphosphate Sufate) sodium borate, yeast protein, water

16. Influenza A (H1N1) Vaccine

(swine flu)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

sodium chloride, monobasic sodium

Influenza A (H1N1) phosphate, dibasic sodium phosphate,
allantoic fluid of chicken eggs, sucrose,
monobasic potassium phosphate,
2009 Monovalent CSL Unlimited beta-Propiolactone, sodium,
potassium chloride, calcium chloride,
Vaccine Nov. 2009 taurodeoxycholate, phosphate buffered
sodium taurodeoxycholate,
-- isotonic solution
ovalbumin, neomycin sulfate,
polymyxin B, beta-propiolactone
Influenza A (H1N1) ID Biomedical allantoic cavity of embryonated hens'
thimerosal, egg
2009 Monovalent Corporation of eggs, formaldehyde, sodium
proteins, formaldehyde, sodium
Vaccine Quebec deoxycholate, phosphate-buffered saline
deoxycholate
-- Jan. 2010 solution
Influenza A (H1N1) MedImmune LLC specific pathogen-free (SPF) chicken monosodium glutamate, hydrolyzed
2009 Monovalent Sep. 2009 eggs, sucrose, potassium phosphate porcine gelatin, arginine, sucrose,
Vaccine dibasic potassium phosphate,
-- monobasic potassium phosphate,
gentamicin sulfate
embryonated chicken
Influenza A (H1N1) eggs, formaldehyde, sucrose density
2009 Monovalent Sanofi Pasteur, Inc solution, polyethylene glycol p- gelatin, formaldehyde, polyethylene
Vaccine Sep. 2009 isooctylphenyl ether (Triton X-100), glycol p-isooctylphenyl ether, sucrose
-- sodium phosphate buffered isotonic
chloride solution

17. Influenza A (H5N1) Vaccine

(bird flu)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

phosphate buffered saline

ID Biomedical thimerosal, squalene, DL-a-
Influenza Virus solution, allantoic cavity of embryonated
Corporation of tocopherol, polysorbate 80,
Vaccine (H5N1) hen's eggs, formaldehyde, sodium
Quebec ovalbumin, formaldehyde, sodium
-- deoxycholate, DL-a-
2013 deoxycholate
tocopherol, polysorbate 80
embryonated chicken
eggs, formaldehyde, linear sucrose
Influenza A (H1N1) porcine
density gradient solution, nonionic
2009 Monovalent Sanofi Pasteur, Inc gelatin, thimerosal, formaldehyde,
surfactant, polyethylene glycol p-
Vaccine 2007 Polyethylene Glycol p-isooctylphenyl
isooctylphenyl ether (Triton X-100),
-- Ether, sucrose
sodium phosphate buffered isotonic
sodium chloride solution

18. Influenza Vaccine

(flu)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Influenza Virus Seqirus Pty. Ltd. beta-propiolactone, sodium thimerosal (multi-dose vials only),
Vaccine 2016-2017 taurodeoxycholate, allantoic fluid of monobasic sodium phosphate, dibasic
Afluria (Trivalent) embryonated chicken eggs, sucrose sodium phosphate, monobasic
density agent, phosphate buffered potassium phosphate, potassium
isotonic solution chloride, calcium chloride, sodium
taurodeoxycholate, neomycin sulfate,
polymyxin B, ovalbumin, mercury,
sodium, chloride, beta-propiolactone,
sucrose
Influenza Virus
Novartis Vaccines kanamycin, neomycin sulfate,
Vaccine formaldehyde, egg
and Diagnostics, cetyltrimethylammonium
Agriflu* protein, polysorbate 80, CTAB,
Inc. bromide, allantoic cavity of embryonated
*This vaccine has been neomycin, kanamycin
Feb. 21, 2013 hens' eggs
discontinued.
MF59C.1 adjuvant
(squalene, polysorbate 80, sorbitan
allantoic cavity of embryonated hen's
trioleate, sodium citrate dihydrate,
Influenza Vaccine, eggs, MF59C.1 adjuvant
Seqiris, Inc. citric acid monohydrate), neomycin,
Adjuvanted (MF59), formaldehyde,
2016-2017 kanamycin, barium, egg
Fluad (Trivalent) cetyltrimethylammonium bromide
proteins, formaldehyde, cetyl
(CTAB)
trimethylammonium bromide
(CTAB)
embryonated chicken eggs, sodium octoxynol-10 (Triton X-100), a-
phosphate-buffered isotonic sodium tocopheryl hydrogen
Influenza Virus GlaxoSmithKline
chloride solution, sodium succinate, polysorbate 80 (Tween
Vaccine Biologicals
deoxycholate, formaldehyde, linear 80), gentamicin sulfate, ovalbumin,
Fluarix Quadrivalent 2016-2017
sucrose density gradient solution hydrocortisone, formaldehyde,
(contains detergent) sodium deoxycholate
embryonated chicken eggs, linear octoxynol-10 (Triton 272 X0100), a-
sucrose density gradient solution tocopheryl hydrogen
Influenza Virus GlaxoSmithKline
(containing detergent), sodium succinate, polysorbate 80 (Tween 80),
Vaccine Biologics
deoxycholate, formaldehyde, sodium hydrocortisone, gentamicin sulfate,
Fluarix Trivalent 2015-2016
phosphate buffered isotonic sodium ovalbumin, formaldehyde, sodium
chloride solution deoxycholate
sodium chloride, monobasic sodium
HA insect cell (fall
Protein Sciences phosphate, dibasic sodium phosphate,
Influenza Vaccine armyworm, Spodoptera frugiperda)
Corporation polysorbate 20 (Tween 20), host cell
Flublok (Trivalent) proteins, lipids, vitamins, amino acids,
2016-2017 proteins, baculovirus, Triton X-100,
mineral salts, Triton X-100
host cell DNA
Influenza Vaccine Seqiris, Inc. Madin Darby Canine Kidney (MDCK) MDCK cell protein, protein other
Flucelvax(Quadrivalent) May 23, 2016 cells, B-propiolactone, than HA, MDCK cell
cetyltrimethylammonium bromide, DNA, polysorbate 80,
phosphate buffered saline cetyltrimethylammonium bromide,
B-propiolactone
MDCK cell protein, other cell
Influenza Virus Madin Darby Canine Kidney (MDCK) proteins, MDCK cell
Seqiris, Inc.
Vaccine cells, B-propiolactone, DNA, polysorbate 80,
Apr. 2016
Flucelvax(Trivalent) cetyltrimethylammonium bromide cetyltrimethylammonium bromide,
B-propiolactone
ID Biomedical
Influenza Virus allantoic cavity of embryonated hens' thimerosal, ovalbumin, formaldehyde,
Corporation of
Vaccine eggs, formaldehyde, sodium sodium deoxycholate, a-tocopheryl
Quebec
FluLaval(Quadrivalent) deoxycholate hydrogen succinate, polysorbate 80
2016-2017
phosphate-buffered saline
ID Biomedical solution, thimerosal (multi-dose
Influenza Virus allantoic cavity of embryonated hens'
Corporation of vaccine
Vaccine eggs, formaldehyde, sodium
Quebec only), ovalbumin, formaldehyde,
FluLaval (Trivalent) deoxycholate
2015-2016 sodium deoxycholate, a-tocopheryl
hydrogen succinate, polysorbate 80
monosodium glutamate, hydrolyzed
porcine gelatin, arginine, sucrose,
Influenza Virus
SPF (specific pathogen-free) eggs, dibasic potassium phosphate,
Vaccine Live, MedImmune, LLC
stabilizing buffer, sucrose, potassium monobasic potassium
Intranasal 2016-2017
phosphate phosphate, ovalbumin, gentamicin
FluMist Quadrivalent
sulfate, ethylenediaminetetraacetic
acid (EDTA)
phosphate-buffered
allantoic cavity of embryonated hens'
Influenza Virus Sequiris Vaccines, saline, thimerosal, egg proteins,
eggs, neomycin, polymyxin,
Vaccine Ltd. polymyxin, neomycin,
betapropiolactone, nonylphenol
Fluvirin 2016-2017 betapropiolactone, nonylphenol
ethoxylate
ethoxylate
Influenza Virus Vaccine Sanofi Pasteur, Inc. embryonated chicken eggs, Octylphenol formaldehyde, octylphenol ethoxylate
Fluzone 2016-2017 Ethoxylate (Triton X-100), sodium (Triton X-100), sodium phosphate-
(Quadrivalent): Standard, High phosphate-buffered isotonic sodium buffered isotonic sodium chloride
Dose, & Intradermal chloride solution, formaldehyde, linear solution, gelatin (standard
sucrose density gradient solution formulation
 only), thimerosal (standard dosage
multi-dose vial only)

19. Japanese Encephalitis Vaccine

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Japanese Encephalitis formaldehyde, bovine serum

Vaccine, Inactivated,
Adsorbed
Ixiaro
Japanese Encephalitis
Vaccine, Inactivated
JE-Vax*
*This vaccine has been
discontinued.
Intercell Vero cells, protamine
albumin, host cell DNA, sodium
Biomedical sulfate, formaldehyde, aluminum
metabisulphite, host cell proteins,
2016 hydroxide
protamine sulphate
Research
thimerosal, Sterile Water for
Foundation for mice (brains are inoculated with the
Injection, gelatin, formaldehyde,
Microbial Diseases virus), phosphate-buffered
v/v Polysorbate 80, mouse serum
of Osaka University saline, formaldehyde, 40% w/v sucrose
protein
Dec. 2005

20. Measles Vaccine

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Measles Virus Vaccine, chick embryo cell culture, buffered salt sorbitol, sodium phosphate, sucrose,
Live solution, vitamins, amino acides, fetal sodium, chloride, hydrolyzed
Merck &Co., Inc.
Attenuvax* bovine serum, SPGA (sucrose, gelatin, human albumin, fetal bovine
Feb. 2006
*This vaccine has been phosphate, glutamate, human albumin), serum, neomycin, other buffer and
discontinued. neomycin media ingredients

21. Meningococcal Vaccine

(meningitis)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Meningococcal Group Novartis Vaccines factor H binding protein (fHbp), Outer kanamycin
B Vaccine and Diagnostics Membrane Vesicles (OMV), aluminum
Bexsero 2015 hydroxide, sodium chloride, histidine,
sucrose, Escherichia coli (E. coli),
deoxycholate
Meningococcal
(Serogroups A, C, Y Mueller Hinton agar, Watson Scherp
and W- media (containing casamino formaldehyde, sodium phosphate
Sanofi Pasteur Inc.
135)Polysaccharide acid), formaldehyde, ammonium sulfate, buffered isotonic sodium chloride
2014
Diphtheria Toxoid modified culture medium (containing solution
Conjugate Vaccine hydrolyzed casein)
MCV4-Menactra
Meningococcal Groups
C and Y and
GlaxoSmithKline semi-synthetic media, formaldehyde,
Haemophilus b Tetanus Tris (trometamol)-HCL,
Biologicals sucrose, saline diluent, sterile saline
Toxoid Conjugate sucrose, formaldehyde, saline diluent
2013 solution
Vaccine
MenHibrix
Meningococcal
(Groups A, C, Y, and
Novartis Vaccines Franz Complete medium, formaldehyde,
W-135)
and Diagnostics, CY medium (contains yeast extracts and
Oligosaccharide formaldehyde
Inc. amino acids), purified polysaccharides,
Diphtheria CRM197
Aug. 2013 CRM197 protein
Conjugate Vaccine
Menveo
Meningococcal
Polysaccharide
distilled water, thimerosal,
Vaccine, Groups A, C, Sanofi Pasteur Inc. Mueller Hinton casein agar, Watson
polysaccharide from serogroups A, C,
Y, W135 Combined 2016 Scherp casamino acid media
Y, and w-135, mercury, lactose
Menomune-A/C/Y/W-
135
Wyeth
Meningococcal Group
Pharmaceuticals, E. coli, defined fermentation growth polysorbate 80 (PS80), AI3+ (as
B Vaccine
Inc. media, polysorbate 80(PS80) AlPO4), mM histidine buffered saline
Trumenba
2016

MMR Vaccine
22.

(measles, mumps, & rubella)

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)
Measles, Mumps, and
Rubella Virus Vaccine,
Live
M-M-R-II
Measles, Mumps,
Rubella and Varicella
Virus Vaccine Live
ProQuad, Refrigerator
Stable
Formulation and Frozen
Formulation
PROPER NAME
COMMERCIAL NAME
(click for package insert)
Pneumococcal 7-valent
Conjugate Vaccine
(Diphtheria CRM197
Protein)
Prevnar*
Merck and Co., Inc. chick embryo cell culture, WI-38 human sorbitol, sodium phosphate, sucrose,
Oct. 2015 diploid lung fibroblasts, Medium 199 sodium chloride, hydrolyzed gelatin,
(containing vitamins, amino acids, fetal recombinant human albumin, fetal
bovine serum, SPGA (sucrose, bovine serum, other buffer and media
phosphate, glutamate, ingredients, neomycin
recombinant human albumin),
neomycin, Minimum Essential Medium
(containing vitamins, amino acids, fetal
bovine serum, recombinant human
albumin, neomycin), sorbitol,
hydrolyzed gelatin stabilizer
sucrose, hydrolyzed gelatin, urea
(refrigerator stable formulation only),
sodium chloride, sorbitol,
monosodium L-glutamate, sodium
phosphate, recombinant human
albumin, sodium bicarbonate,
potassium phosphate, potassium
chick embryo cell culture, WI-38 human
chloride, components of MRC-5
Merck and Co., Inc. diploid lung fibroblasts, MRC-5
cells (including DNA and protein),
Oct. 2015 cells, bovine serum, recombinant human
neomycin, bovine serum albumin,
albumin
other buffer and media ingredients,
sodium phosphate dibasic (frozen
formulation only), potassium
phosphate monobasic (frozen
formulation only), potassium
phosphate dibasic (frozen formulation
only)
23. Pneumococcal Vaccine
(pneumonia)
MANUFACTURER VACCINE INGREDIENTS
GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
Wyeth soy peptone broth, casamino acids and aluminum (as aluminum phosphate
Pharmaceuticals yeast extract-based medium, ammonium adjuvant)
Inc. sulfate, saccharides
Mar. 2009
*This vaccine has been
discontinued.
Pneumococcal 13-
Wyeth
valent Conjugate soy peptone broth, casamino acids and polysorbate 80, succinate
Pharmaceuticals
Vaccine (Diphtheria yeast extract-based medium, ammonium buffer, aluminum (as aluminum
Inc.
CRM197 Protein) sulfate, CRM 197 phosphate adjuvant)
2016
Prevnar 13
Pneumococcal
Merck & Co., Inc.
Vaccine, Polyvalent -- isotonic saline solution, phenol
May 2014
Pneumovax-23

24. Polio Vaccine

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Poliovirus Vaccine
Vero cells, Eagle MEM modified 2-phenoxyethanol, formaldehyde,
Inactivated (Monkey Sanofi Pasteur, SA
medium, newborn calf serum, M-199 neomycin, streptomycin, polymyxin
Kidney Cell) 2015
(without calf serum) B, calf bovine serum albumin
IPOL

25. Rabies Vaccine

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Rabies Vaccine Sanofi Pasteur, SA human albumin, neomycin sulfate,

MRC-5 cells, beta-propiolactone phenol red indicator, beta-
IMOVAX Apr. 25, 2013
propiolactone
polygeline (processed bovine
chicken fibroblasts, synthetic cell culture
gelatin), human serum albumin,
Novartis Vaccines medium (with added human
Rabies Vaccine potassium glutamate, sodium
and Diagnostics albumin), polygeline (processed bovine
EDTA, bovine serum, chicken
RabAvert gelatin), antibiotics, B-propiolactone,
Nov. 2013 protein, chicken ovalbumin,
sucrose density-gradient, buffered
neomycin, chlortetracycline,
polygeline, potassium glutamate
amphotericin B, water for injection

26. Rotavirus Vaccine

 PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

sucrose, sodium citrate, sodium

Rotavirus Vaccine, phosphate monobasic monohydrate,
Merck & Co., Inc. Vero cells, buffered stabilizer, porcine-
Live, Oral, Pentavalent sodium hydroxide, polysorbate 80,
June 2013 derived materials
RotaTeq cell culture media, fetal bovine
serum, DNA for porcine circoviruses
Vero cells, porcine-derived materials,
amino acids, dextran, Dulbecco's
Modified Eagle Medium (sodium
chloride, potassium chloride, magnesium
Rotavirus Vaccine, GlaxoSmithKline sulfate, ferric (III) nitrate, sodium
calcium carbonate, sterile water,
Live, Oral Biologicals phosphate, sodium pyruvate, D-glucose,
xanthan, porcine cirrovirus type 1
Rotarix 2016 concentrated vitamin solution, L-cystine,
L-tyrosine, amino acids solution, L-
glutamine, calcium chloride, sodium
hydrogenocarbonate, phenol red),
sorbitol, sucrose

27. Rubella Vaccine

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

WI-38 human diploid lung

Rubella Virus Vaccine fibroblasts, Minimum Essential sorbitol, sodium phosphate, sucrose,
Live Medium (MEM; buffered salt solution sodium chloride, hydrolyzed
Merck & Co., Inc.
MERUVAX II* (vitamins, amino acids, bovine gelatin, human albumin, fetal bovine
Feb. 2006
*This vaccine has been serum), human serum albumin, serum, other buffer and media
discontinued. neomycin), sorbitol, hydrolyzed gelatin ingredients, neomycin
stabilizer

28. Smallpox Vaccine

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Smallpox (Vaccinia) Sanofi Pasteur Vero cells, human serum albumin, glycerin, phenol in Water for
Vaccine, Live Biologics Co. sodium chloride, mannitol, neomycin, Injection
ACAM2000 Dec. 2015 polymyxin B, Dryvax

29.TD Vaccine
(tetanus & diphtheria)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

Tetanus and Diphtheria

Toxoids Adsorbed For
Mueller and Miller medium, peptone-
Adult Use Sanofi Pasteur, Inc. thimerosal, aluminum potassium
based medium, extract of bovine muscle
DECAVAC* Mar. 2011 sulfate adjuvant, formaldehyde
tissue, formaldehyde, ammonium sulfate
*This vaccine has been
discontinued.

Tetanus and Diphtheria modified Mueller-Miller casamino acid

medium (without beef heart
Toxoids Adsorbed For Sanofi Pasteur, Ltd. aluminum phosphate, formaldehyde,
infusion), formaldehyde, ammonium
Adult Use Apr. 2013 sodium chloride, water for injection
sulfate, modified Mueller's growth
TENIVAC
medium, aluminum phosphate

Tetanus and Diphtheria modified Mueller's

Mass Biologics media (contains bovine aluminum
Toxoids, Adsorbed
Feb. 2011 extracts), formaldehyde, ammonium adjuvant, formaldehyde, thimerosal
--
sulfate, aluminum phosphate
Tetanus Toxoid
peptone-based medium, formaldehyde,
--* Sanofi Pasteur, Inc.
ammonium sulfate, physiological saline thimerosal, formaldehyde
*This vaccine has been Dec. 2005 solution
discontinued.
Tetanus Toxoid
peptone-based medium (contains extract
Adsorbed
Sanofi Pasteur, Inc. ofbovine muscle tissue), formaldehyde, thimerosal, physiological saline
--* ammonium sulfate, aluminum potassium solution
July 2005
*This vaccine has been sulfate (alum)
discontinued.

Tdap Vaccine
30.

(tetanus, diphtheria, & pertussis)

PROPER NAME MANUFACTURER GROWTH MEDIUMS & PROCESS INGREDIENTS VACCINE INGREDIENTS
(not in order of quantity; see package insert for
 COMMERCIAL NAME PACKAGE INSERT DATE quantities)
(click for package insert)

modified Stainer-Scholte medium (added

Tetanus Toxoid,
Reduced Diphtheria
Toxoid and Acellular
Pertussis Vaccine,
Adsorbed
Adacel
Tetanus Toxoid,
Reduced Diphtheria
Toxoid and Acellular
Pertussis Vaccine,
Adsorbed
Boostrix
casamino acid, dimethyl-beta-
cyclodextrin),
Sanofi Pasteur, Ltd. glutaraldehyde, formaldehyde, aluminum aluminum phosphate, formaldehyde,
glutaraldehyde, 2-phenoxyethanol,
2015 phosphate, modified Mueller-Miller
water for injection
casamino acid medium (without beef
heart infusion), ammonium sulfate, 2-
phenoxyethanol, water for injection
modified Latham medium derived
from bovine casein, Fenton
GlaxoSmithKline aluminum hydroxide, sodium
medium (containing bovine
Biologicals chloride, formaldehyde, polysorbate
extract), formaldehyde, modified
2016 80 (Tween 80)
Stainer-Scholte liquid medium,
glutaraldehyde, aluminum hydroxide

31. Typhoid Vaccine

PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)

formaldehyde, phenol,
polydimethylsiloxane or fatty-acid
Typhoid Vi ester-cased antifoam, isotonic
Sanofi Pasteur SA semi-synthetic medium,
Polysaccharide Vaccine phosphate buffered saline, sodium
Mar. 2014 hexadecyltrimethylammonium bromide
Typhim Vi chloride, disodium phosphate,
monosodium phosphate, sterile water
for injection, vi polysaccharide
Typhoid Vaccine Live yeast extract, casein, dextrose, and
Berna Biotech, Ltd. sucrose, ascorbic acid, amino acid
Oral Ty21a galactose, sucrose, ascorbic acid, amino
Sep. 2013 mixture, lactose, magnesium stearate
Vivotif acids, lactose, magnesium stearate

32. Varicella Vaccine

(chickenpox)
PROPER NAME MANUFACTURER VACCINE INGREDIENTS
COMMERCIAL NAME GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
(click for package insert)
Varicella Virus Vaccine, Live
Varivax, Frozen and Refigerated
PROPER NAME
COMMERCIAL NAME
(click for package insert)
Yellow Fever Vaccine
YF-Vax
PROPER NAME
COMMERCIAL NAME
(click for package insert)
Zoster Vaccine, Live
Merck & Co., Inc.
Zostavax, Frozenand Refrigerator
Feb. 2016
Stable
Merck & Co., Inc. human embryonic lung cell cultures, sucrose, hydrolyzed gelatin, urea
Dec. 2012 embryonic guinea pig cell cultures, WI- (refrigerated vaccine only), sodium
38 human diploid cell cultures, MRC-5 chloride, monosodium L-glutamate,
human diploid cell cultures, sucrose, sodium phosphate dibasic, potassium
phosphate, glutamate, processed gelatin, phosphate monobasic, potassium
urea (refrigerated vaccine only) chloride, components of MRC-5
cells (DNA, protein),
neomycin, bovine calf serum, sodium
phosphate monobasic, EDTA, fetal
bovine serum
33. Yellow Fever Vaccine
MANUFACTURER VACCINE INGREDIENTS
GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
Sanofi Pasteur, Inc. living avian leukosis virus-free (ALV- sorbitol, gelatin, sterile diluent
June 2016 free) chicken embryos (sodium chloride)
34. Zoster Vaccine
(shingles)
MANUFACTURER VACCINE INGREDIENTS
GROWTH MEDIUMS & PROCESS INGREDIENTS (not in order of quantity; see package insert for
PACKAGE INSERT DATE quantities)
sucrose, hydrolyzed porcine gelatin,
sodium chloride, monosodium L-
glutamate, sodium phosphate dibasic,
potassium phosphate monobasic,
--
potassium chloride, components
of MRC-5 cells (DNA, protein),
neomycin, bovine calf serum, urea
(refrigerator stable only)
II. GLOSSARY AND DETAILS FOR INGREDIENTS

Possible Ingredients
Product
(Ingredients depend on which modification is used.)

2- 2-Phenoxyethanol is a glycol ether used as a preservative in vaccines.

Phenoxyethanol

Aluminum Aluminum is used in vaccines as an adjuvant, which helps the vaccine work more quickly and
more powerfully.

Bovine casein A casein is a family of phosphoproteins commonly found in mammalian milk. 80% of the
proteins in cow's milk are casein.

Bovine serum Bovine "[s]erum is the centrifuged fluid component of either clotted or defibrinated whole blood.
Bovine serum comes from blood taken from domestic cattle. Serum from other animals is also
collected and processed but bovine serum is processed in the greatest volume."

"Bovine serum is a by-product of the meat industry. Bovine blood may be taken at the time of
slaughter, from adult cattle, calves, very young calves or (when cows that are slaughtered are
subsequently found to be pregnant) from bovine fetuses. It is also obtained from what are called
'donor' animals, which give blood more than once.
 Blood is available from bovine fetuses only because a proportion of female animals that are
slaughtered for meat for human consumption are found (often unexpectedly) to be pregnant.

Blood is available from very young calves because calves, especially males from dairy breeds, are
often slaughtered soon, but not necessarily immediately, after birth because raising them will not
be economically beneficial. Older animals are, of course, slaughtered for meat.

Only donor cattle are raised for the purpose of blood donation. Donor cattle are invariably kept in
specialized, controlled herds. Blood is taken from these animals in a very similar way to that used
for human blood donation.

Irrespective of whether blood is taken at slaughter or from donors, the age of the animal is an
important consideration because it impacts the characteristics of the serum.

Bovine serum is categorised according to the age of the animal from which the blood was
collected as follows:
'Fetal bovine serum' comes from fetuses
'Newborn calf serum' comes from calves less than three weeks old
'Calf serum' comes from calves aged between three weeks and 12 months
'Adult bovine serum' comes from cattle older than 12 months

Serum processed from donor blood is termed 'donor bovine serum'. Donor animals can be up to
three years old."

Chicken Eggs Viruses can be grown in chicken eggs before being used in vaccinations.

CMRL-1969 L-alanine, L-arginine (free base)b, L-aspartic acid, L-cysteine-HCL, L-cystine, L-glutamic acid-
H20, L-glutamine, glycine, L-histidine (free base)b, L-hydroxyproline, L-isoleucine, L-leucine, L-
lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-
tyrosine, L-valine, p-aminobenzoic acid, ascorbic acid, d-biotin, calcium pantothenate,
cholesterol, choline chloride, ethanol, folic acid, glutathione, i-inositol, menadione, nicotinamide,
nicotinic acid, pyridoxal-HCL, pyridoxine-HCL, riboflavine, riboflavine-5-phosphate, sodium
acetate-3H2O, thiamine-HCL, Tween 80, vitamin A acetate, vitamin D (calciferol), vitamin E (a-
tocopherol phosphate), D-glucose, phenol red, sodium chloride, potassium chloride, calcium
chloride, magnesium culphate heptahydrate, sodium phosphate dibasic, sodium dihydrogen
phosphate, monopotassium phosphate, sodium bicarbonate, iron nitrate nonahydrate

Dulbecco's glucose, sodium bicarbonate, L-glutamine, pyridoxine HCl, pyridocal HCl, folic acid, phenol red,
Modified HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid), L-methionine, L-cystine,
Eagle's Serum sodium phosphate mono-basic, sodium pyruvate, vitamins

Earle's inorganic salts, D-glucose, phenol red, calcium, magnesium salts

Balanced Salt
Medium

Fenton Medium bovine extract

Formaldehyde Formaldehyde is used in vaccines to inactivate the virus so the person being inoculated does not
contract the disease.

Human albumin Human albumin is a blood plasma protein produced in the liver that, among other functions,
transports hormones, fatty acids, and other compounds, and buffers pH.

Insect Cells Cabbage moth and fall armyworm cells are used to grow viruses for vaccines.

Latham bovine casein

Medium

MDCK cells from normal female adult Cocker Spaniel (harvested in 1958 by SH Madin and NB Darby),
(Madin-Carby EMEM(EBSS) (Eagle's Minimum Essential Medium with Earle's Balanced Salt Solution),
canine kidney glutamine, non essential amino acids, foetal bovine serum
cells)

Mouse Brains Live mice brains are inoculated with the Japanese encephalitis virus to grow the virus used in the
vaccine.

MRC-5 Medical Research Council 5, human diploid cells (cells containing two sets of chromosomes)
derived from the normal lung tissues of a 14-week-old male fetus aborted for "psychiatric
reasons" in 1966 in the United Kingdom, Eagle's Basal Medium in Earle's balanced salt solution
with bovine serum.

Mueller Hinton beef extract, acid hydrolysate of casein, starch, agar

Agar

Mueller-Miller glucose, sodium chloride, sodium phosphate dibasic, monopotassium, phosphate, magnesium
Medium sulfate hydrate, ferrous sulfate heptaphydrate, cystine hydrochloride, tyrosine hydrochloride,
urasil hydrochloride, Ca-pantothenate in ethanol, thiamine in ethanol, pyridoxin-hydrochloride in
 ethanol, riboflavin in ethanol, biotin in ethanol, sodium hydroxide, beef heart infusion (de-fatted
beef heart and distilled water), casein solution

Polysorbate 80 Also called Tween 80, Alkest 80, or Canarcel 80 (brand names). Polysorbate 80 is used as an
excipient (something to basically thicken a vaccine for proper dosing) and an emulsifier
(something to bond the ingredients).

Porcine gelatin Gelatin is used to protect viruses in vaccines from freeze-drying or heat and to stabilize vaccines
so they stay stable.

Stainer-Scholte tris hydrochloride, tris base, glutamate (monosodium salt), proline, salt, monopotassium
Liquid Medium phosphate, potassium chloride, magnesium chloride, calcium chloride, ferrous sulfate, ascorbic
acid, niacin, glutathione

Thimerosal Thimerosal is an organomercury compound used as a preservative.

Vero Cells cells derived from the kidney of a normal, adult African Green monkey in 1962 by Y. Yasumura
(African Green and Y. Kawakita
Monkey Cells)

WI-38 human Winstar Institute 38, human diploid lung fibroblasts derived from the lung tissues of a female
diploid cells fetus aborted because the family felt they had too many children in 1964 in the United States

III. SOURCES

Acumedia Manufacturers, "Mueller Hinton Agar (7101)," www.neogen.com, June 2011

Atlanta Biologicals, "Earle's Balanced Salt Solution (EBSS)," www.atlantabio.com, 2010

CDC, "Basics and Common Questions: Ingredients of Vaccines - Fact Sheet," www.cdc.gov, Feb. 22, 2011

FDA, "Vaccines Licensed for Immunization and Distribution in the US with Supporting Documents," www.fda.gov, Aug. 29, 2016

Health Protection Agency, "General Cell Collection: MDCK," www.hpacultures.org.uk, 2011

G.M. Healy, S. Teleki, A.V. Seefried, M.J. Walton, and H.G. Macmorine, "Improved Chemically Defined Basal Medium (CMRL-1969) for
Primary Monkey Kidney and Human Diploid Cells," Applied and Environmental Microbiology, www.aem.asm.org, 1971

International Serum Industry Association, "FAQ," www.serumindustry.org/faq, 2013

Pontifical Academy for Life, "Moral Reflections on Vaccines Prepared From Cells Derived From Aborted Human Foetuses,"
www.immunize.org/concerns/vaticandocument.htm, June 9, 2005

Rebecca Sheets, "History and Characterization of the Vero Cell Line," www.fda.gov, May 12, 2000

Sigma-Aldrich, "DMEM," www.sigmaaldrich.com, 2013

Alison Weiss, "The Genus Bordetella," The Prokaryotes: A Handbook on the Biology of Bacteria," Ed. Martin M. Dworkin, Stanley
Falkow, Karl-Heinz Schleifer, and Erko Stackebrandt, 2006.

World Health Organization, "Production and Control of Tetanus Vaccine: A Training Curriculum, Module III: Principles of Tetanus Vaccine
Production," www.who.int, Sep. 3, 1999