#1 NAME____________________________________

MCB 2004
GENERAL MICROBIOLOGY
TEST #1, FEBRUARY 5, 2001

IN THIS PART THERE ARE FORTY-FIVE QUESTIONS WORTH TWO POINTS EACH.
THERE IS ONLY ONE CORRECT ANSWER FOR EACH QUESTION. PLEASE CIRCLE
THE CORRECT LETTER OR PLACE THE CORRECT LETTER JUST TO THE LEFT OF
THE QUESTION NUMBER. THEN TRANSFER ALL YOUR ANSWERS TO THE ANSWER
SHEET AT THE END OF THE TEST PACKET.

KEY FOR QUESTIONS 1-7. THERE IS ONLY ONE CORRECT ANSWER BUT EACH KEY
WORD MAY BE USED MORE THAN ONCE (OR NOT AT ALL).

A. MITOCHONDRION
B. GOLGI APPARATUS
C. NUCLEUS
D. PILUS
E. RIBOSOME

_____1. FOUND ONLY IN PROCARYOTES

_____2. COMPOSED OF THREE RNAs AND ABOUT 50 PROTEINS

_____3. FOUND IN BOTH PROCARYOTES AND EUCARYOTES

_____4. APPENDAGE USED FOR ATTACHMENT

_____5. THE SITE OF PROTEIN SYNTHESIS

_____6. THE LARGEST AND MOST OBVIOUS ORGANELLE IN EUKARYOTIC CELLS

_____7. ORGANELLE RESPONSIBLE FOR ENERGY PRODUCTION

8. IN THE GRAM STAIN THE COUNTER STAIN IS _______.

A. BASIC FUCHSIN
B. IODINE
C. CRYSTAL VIOLET
D. WATER
E. ACETONE OR ALCOHOL

9. STAINING FIXED CELLS ONLY WITH METHYLENE BLUE IS AN EXAMPLE OF:

A. SPECIAL STAINING
B. SIMPLE STAINING
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE
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10. IN BRIGHT FIELD MICROSCOPY LIGHT PASSING THROUGH THE

SPECIMEN (ON THE STAGE) NEXT PASSES THROUGH THE
A. OBJECTIVE LENS
B. LIGHT SOURCE
C. CONDENSER
D. OCULAR LENS

11. THE ACID FAST "OUTER MEMBRANE" IS UNUSUALLY THICK BECAUSE OF THE
A. MYCOLIC ACIDS
B. LIPOPOLYSACCHARIDES
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE

12. THE EXPONENTIAL PHASE OF BACTERIAL GROWTH IS ALSO CALLED THE

A. STATIONARY PHASE
B. LOG PHASE
C. LAG PHASE
D. DEATH PHASE
E. REPLICATION PHASE

13. MOST HUMAN PATHOGENS ARE

A. BAROPHILES
B. THERMOPHILES
C. BASOPHILES
D. MESOPHILES
E. PSYCHROPHILES
o
14. THE DOUBLING TIME OF E. COLI IN NUTRIENT BROTH AT 37 C IS
30 MINUTES. THE INITIAL INOCULUM OF THE CULTURE CONTAINS 500
CELLS. AFTER TWO HOURS OF EXPONENTIAL GROWTH THE CULTURE CONTAINS
A. 1000 CELLS
B. 4000 CELLS
C. 5000 CELLS
D. 8000 CELLS
E. 10000 CELLS

15. A BACTERIUM THAT OXIDIZES SULFUR WITH OXYGEN FOR ENERGY AND
USES CARBON DIOXIDE AS ITS CARBON SOURCE IS A
A. CHEMOAUTOTROPH
B. STRICT ANAEROBE
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE

16. THE FOLLOWING IS ONE INGREDIENT OF A DEFINED MEDIUM

A. NUTRIENT AGAR
B. GLUCOSE
C. PEPTONE
D. YEAST EXTRACT
E. BEEF INFUSION

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17. HIS DISCOVERY IN 1953 EARNED HIM THE NOBEL PRIZE AND MARKED
THE BEGINNING OF THE AGE OF MOLECULAR BIOLOGY.
A. ROBERT KOCH
B. JAMES WATSON
C. VAN LEEUWENHOEK
D. EDWARD JENNER
E. LOUIS PASTEUR

18. PEPTIDOGLYCAN IS MADE OF POLYMERS OF TWO SUGARS CROSSED-LINKED BY

A. LIPIDS
B. PEPTIDE CHAINS
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE

19. IN 1800 50% OF THE U.S. POPULATION DIED OF INFECTIOUS DISEASES;

25% DIED OF
A. INFLUENZA
B. SMALLPOX
C POLIO
D. TUBERCULOSIS

20. THE LIGHT MICROSCOPE IS CAPABLE OF ____ RESOLUTION, AND ____

MAGNIFICATION.
A. 0.4 mm, 10 X
B. 10 µm, 100 X
C. 0.2 µm, 2,000 X
D. 2 nm , 100,000 X

21. ONE METER EQUALS

A. 1000 mm
B. 1,000,000 µm
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE

22. THE DARKFIELD MICROSCOPE IS USEFUL FOR

A. SEEING BACTERIAL FLAGELLA
B. DIAGNOSING SYPHILIS
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE

23. THE NUMBER OF BACTERIA PER ML IN THIS CULTURE FLASK IS

A. 42,000
B. 210,000
C. 420,000
D. 2,100,000
E. 4,200,000
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KEY FOR QUESTIONS 24-30. THERE IS ONLY ONE CORRECT ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).

A. ESCHERICHIA COLI
B. STAPHYLOCOCCUS AUREUS
C. MYCOBACTERIUM LEPRAE
D. TREPONEMA PALLIDUM
E. CLAMYDIA TRACHOMATIS

_____24. CONTAINS LIPOPOLYSACCHARIDE (LPS)

_____25. ITS ENVELOPE HAS TWO LAYERS, A PLASMA MEMBRANE AND A THICK
PEPTIDOGLYCAN LAYER

_____26. IT HAS A CELL WALL THAT DOES NOT CONTAIN PEPTIDOGLYCAN

_____27. IT HAS A COILED SHAPE AND AXIAL FILAMENTS

_____28. ITS CLOSE RELATIVE IS PSEUDOMONAS AERUGINOSA

_____29. CONTAINS MYCOLIC ACIDS AND ARABINOGLACTANS IN ITS ENVELOPE

_____30. GROWS AS SPHERICAL CELLS IN CLUSTERS

31. KILLING ALL ORGANISMS INCLUDING SPORES PRESENT IN A MATERIAL

IS KNOWN AS
A. FILTRATION
B. INCINERATION
C. STERILIZATION
D. LYOPHILIZATION
E. PASTEURIZATION

32. MANY ANTIBIOTICS AND VITAMINS WHICH ARE UNSTABLE TO HEAT ARE
____________ BEFORE THEY ARE ADDED TO BACTERIAL GROWTH MEDIA
A. AUTOCLAVED
B. PASTEURIZED
C. FROZEN
D. FILTERED
E. BOILED

33. ORGANIC COMPOUNDS LIKE ISOPROPANOL PROBABLY HAVE THEIR GREATEST

BACTERIAL KILLING EFFECT BY
A. HYDROLYZING DNA
B. DESTROYING PROTEINS
C. DISRUPTING PLASMA MEMBRANES
D. DISRUPTING SPORES
E. DISRUPTING RIBOSOMES

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34. HIS OBSERVATION THAT MILKMAIDS WERE IMMUNE TO SMALLPOX LED TO
THE FIRST USEFUL VACCINE
A. JONAS SALK
B. LOUIS PASTEUR
C. ALEXANDER FLEMING
D. PAUL EHRLICH
E. EDWARD JENNER

35. EUCARYOTIC CELLS ARRESTED JUST BEFORE MITOSIS ARE IN

A. Go
B. G1
C. G2
D. M
E. S

36.

37. A BACTERIAL FLAGELLUM IS MADE UP OF THREE PARTS. THEY INCLUDE

A. THE HOOK
B. THE MICROTUBULES
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE

38. IF YOU WANTED TO STERILIZE PLASTIC-WARE (LIKE YOUR PLASTIC

PETRI DISHES OR DISPOSABLE PIPETS) YOU COULD USE
A. AUTOCLAVING
B. ETHYLENE OXIDE
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE

39. THE LARGE SUBUNIT OF THE BACTERIAL RIBOSOME CONTAINS

A. 23S RNA, 16S RNA AND 30 PROTEINS
B. 23S RNA, 5S RNA AND 30 PROTEINS
C. 16S RNA, 5S RNA AND 20 PROTEINS
D. 16S RNA, 5S RNA AND 20 PROTEINS
E. 16S RNA AND 20 PROTEINS

40. THE PORTION OF LIPOPOLYSACCHARIDE (LPS) MADE OF REPEATING SUGARS

IS THE
A. THE O ANTIGEN
B. THE CORE POLYSACCHARIDE
C. THE ENZYMATIC PORTION
D. LIPID A
E. THE CELL-BINDING PORTION

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KEY FOR QUESTIONS 41-45. EACH QUESTION HAS ONLY ONE ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).

A. THE TCA CYCLE

B. PROTEIN SYNTHESIS
 C. LACTIC ACID FERMENTATION
D. THE ELECTRON TRANSPORT CHAIN
E. HISTIDINE BIOSYNTHETIC PATHWAY

_____41. ANABOLISM OF AN AMINO ACID

_____42. IN THE FIRST STEP, OXALOACETIC ACID ACCEPTS ACETATE FROM

ACETYL-COA TO PRODUCE CITRIC ACID

_____43. ANABOLISM OF A MACROMOLECULE

_____44. THE OXIDATION OF NADH BY MOLECULAR OXYGEN

_____45. ONLY TWO ATPS ARE PRODUCED FROM ONE GLUCOSE IN THIS PROCESS

ANSWER ONE OF THE FOLLOWING THREE QUESTIONS (10 POINTS)

46. DRAW A TYPICAL BACTERIAL GROWTH CURVE AND LABEL IT (DON'T

FORGET TO LABEL THE AXES.).
OR
47. DRAW A DIAGRAM OF A GRAM NEGATIVE CELL ENVELOPE AND LABEL IT.
OR
48. DRAW AND LABEL THE METABOLIC PATHWAYS (DIAGRAMATICALLY) FROM
GLUCOSE TO CO2 AND H20 USING MOLECULAR OXYGEN. INCLUDE ATP
PRODUCTION.