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CH1131_BiomolecularEngineering_Tutorial1_Questions
25,26,28Aug2015
1. ItmakessensethatDNA,thegeneticmaterialofmostorganisms,shouldbesequesteredina
specificareaofthecelltoprotectitandpreventitfrompotentialdamage.Whatisan
advantageofmakingthemRNA,theworkingcopyofthegeneticcode,somuchmorelabile
thanDNA?

2. Youdiscoveramutantorganismthathasdifficultytranscribingaparticularimportantgene.
Itappearsthatwhentranscriptionofthisgeneshouldbeoccurring,RNApolymerasehas
difficultydeterminingwheretranscriptionshouldstart.WhatDNAsequenceismostlikelyto
beresponsibleforthisproblem?

3. MostmRNAscodingforproteinhavelongstretchesofadenineribonucleotides[poly(A)]on
the3'endofthemessage.DoesthatmeanthateachgenefromwhichthesemRNAsare
derivedhasacorrespondingstretchofpoly(dT)atthe5'endofthetemplatefromwhichthe
mRNAwasformed?

4. Thebuildingblocksofpolymersarecalled_______.

a. minipolymers

b. monoblocks

c. monomers

d. portions

e. octamers

9. Whichofthefollowingisanucleoside?

a. phosphate+ribose

b. adenine+deoxyribose

c. sugar+nitrogenousbase

d. adenine+ribose+phosphate

e. bandc

10. Whichofthefollowingisanucleotide?

a. phosphate+ribose

b. adenine+deoxyribose

c. sugar+nitrogenousbase

d. adenine+ribose+phosphate
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e. bandc

11. 35.TheDNAbuildingblocksareknownas________.

a. phosphodiesters

b. nucleosides

c. nucleotides

d. phospholipids

e. aminoacids

12. 36.Thebuildingblocksofanucleotideare___________.

a. apentosesugarandaphosphategroup

b. apentosesugarandanitrogenousbase

c. aphosphategroupandanitrogenousbase

d. apentosesugar,aphosphategroupandanitrogenousbase

e. apentosesugar,aphosphategroupandanaminoacid

13. Purinesarecharacterizedby_______;pyrimidinesarecharacterizedby______.

a. tworings;onering

b. onering;tworings

c. tworings;threerings

d. tworings;tworings

e. tworings;onechain

14. ThetwostrandsoftheDNAdoublehelixarecharacterizedasbeing________.

a. antiparallel

b. complementarytoeachother

c. identicaltoeachother

d. parallel

e. aandb
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15. WhatgivesaDNAmoleculeitsnegativecharge?

a. deoxyribose

b. ribose

c. phosphategroups

d. chlorineion

e. adenine

16. ThetwochainsoftheDNAdoublehelixareheldtogetherby______.

a. hydrophobicinteractions

b. hydrophilicinteractions

c. vanderWaalsinteractions

d. Hbonds

e. aandc

17. WhatisanadvantageofmakingmRNAcopiesofgenes?

a. ThegeneticinformationcanbestoredrelativelysafelyinthenucleuswhilemRNAisa
workingcopy..

b. mRNAissmallerthanDNAandeasiertomoveoutintothecytoplasmwhereproteinsare
made.

c. MakingmultiplecopiesofmRNAallowsanamplificationofsyntheticoutputoverwhat
couldbemadefromonegeneinDNA.

d. mRNAshaveshorthalflivesallowingacelltochangeitsresponsestotheenvironment
efficiently.

e. alloftheabove

18. Ribosomesaremadeof_______.

a. proteins

b. lipids

c. tRNA

d. rRNA

e. aandd
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19. ThesiteonDNAtowhichRNApolymerasesbindbeforeinitiatingtranscriptioniscalledthe
______.

a. terminator

b. operator

c. promoter

d. enhancer

e. silencer

20. ProteinsthathelpRNApolymeraserecognizepromotersarecalled__________.

a. transcriptionfactors

b. translationfactors

c. elongationfactors

d. proteases

e. aandc

21. Eukaryoteshavearequirementforalargevarietyofaccessoryproteinstohelpthemcarry
outtheprocessoftranscription.Whataretheseproteinscalled?

a. transcriptionals

b. elastins

c. transcriptionfactors

d. elongationfactors

e. transcriptionpolypeptides

22. Whatisthefunctionofthepoly(A)tailonmostmRNAs?

a. tohastendegradationofthemRNA

b. topreventprematuredegradationofthemRNAbyexonucleases

c. topreventprematuredegradationofthemRNAbyendonucleases

d. topreventviralinfections

e. todecreasethestabilityofmRNA
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23. Youhaveattachedsyntheticpoly(T)toachromatographycolumn.Ifyoupassamixtureof
cellularRNAsthroughthecolumn,whattypeofRNAwillbeselectivelyretainedbythe
columnandofwhattypeofchromatographyisthistechniqueanexample?

a. rRNAs,affinitychromatography

b. mRNAs,affinitychromatography

c. mRNAs,ionexchangechromatography

d. mRNAs,gelfiltration

e. rRNAs,affinitychromatography