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ABSTRACT
INTRODUCTION
MATERIALS A N D METHODS
The marine microalga used was Tetraselmis suecica, isolated from Ria de
Arosa waters (NW Spain). It was cultured in seawater filtered through a
0-45/~m Millipore filter, autoclaved at 120C for 20 min and enriched
with the different nutrients. We used four types of nutrients: common
garden fertilizer, Ferticros 7-12-7 (F); common garden fertilizer+soil
extract (F+ SE); common garden fertilizer + soil extract + micronutrient
(F+ SE + M); common garden fertilizer + micronutrient vitamins
(F+ M + V ) . As a blank, we used a culture with the inorganic medium
commonly used in our laboratory (Fabregas and Herrero, 1985;
Fabregas et al., 1984a, 1985a). All the experiments were carried out in
triplicate.
Biomass production of the marine microalga Tetraselmis suecica 143
3
o
~2 x2
35
-~%3NS
5 35'
~'>'~o_~'%-%\ ~ X \ \'~>-,~ ~ ? /
>7 \
TlvlEk:lays) TIME(days)
Fig. 2. Cellular densities of cultures of T. suecica with different mixtures and concen-
trations of nutrients: A, fertilizer; B, fertilizer+soil extract; C, fertilizer+soil
extract + micronutrients; D, fertilizer + micronutrients + vitamins.
TABLE 1
Growth and Protein and Chlorophyll a Concentrations of Cultures of T. suecica with
Different Mixtures and Concentrations of Nutrients
160 -
,'-,120-
,~ " B C D
80"
z
oI ~ 40
Q.
O1
v
-1 E
.._18'
_J
),.
A B D
~F 6
.,)
The fertilizer Ferticros does not contain all the elements necessary for
maximum microalgal growth, although it can be used as a source of
nitrogen and phosphorus.
The use of soil extract for marine microalgal growth in seawater is
very common (Prakash and Rashid, 1968; Prakash et al., 1973). This soil
extract can supply vitamins, micronutrients and, perhaps, certain other
growth factors, acting besides as a chelating agent (Hoeffner and
Manahan, 1980) because humic substances chelate ions of Fe, Ca, A1,
Cu and Co, so that these ions are prevented from precipitating essential
microalgal nutrients such as phosphate (Prakash et al., 1973); on the
148 J. Fabregas, L. Toribio, J. Abalde, B. Cabezas, C. Herrero
t-
t~
~d
+ +
U~ t~ r~
rr_
+ Jr + <
Biomassproduction of the marine microalga Tetraseimissuecica 149
ACKNOWI .EDGEMENTS
REFERENCES
Bayne, B. L. (1976). The biology of the mussel larvae. In: Marine Mussels: Their
Ecology and Physiology, ed. B. L. Bayne, Cambridge University Press,
Cambridge, pp. 81-120.
Bradford, M. (1976). A rapid and sensitive method for the quantitation of
microgram quantities of protein utilizing the principle of protein dye-binding.
Anal Biochem., 72, 248-54.
De Pauw, N., Verboven, J. & Claus, Co (1983). Large-scale microalgae produc-
tion for nursery rearing of marine bivalves. Aquacultural Engineering, 2,
27-47.
Fabregas, J. & Herrero, C. (1985), Marine microalgae as a potential source of
Single Cell Protein (SCP). Appl. Microbiol. Technol., 23, 110-13.
Fabregas, J., Abalde, J., Herrero, C., Cabezas, B. & Veiga, M. (1984a). Growth
of the marine microalga Tetraselmis suecica in batch cultures with different
salinities and nutrient concentrations. Aquaculture, 42, 207-15.
Fabregas, J., Herrero, C. & Veiga, M. (1984b). Effect of oil and dispersant on
growth and chlorophyll a content of the marine microalga Tetraselmis
suecica. Appl. Environ. Microbiol., 47(2), 445-7.
150 J. Fabregas, L. Toribio, J. Abalde, B. Cabezas, C. Herrero
Fabregas, J., Herrero, C., Cabezas, B. & Abalde, J. (1985a). Mass culture and
biochemical variability of the marine microalga Tetraselmis suecica Kylin
(Butch) with high nutrient concentrations. Aquaculture, 49, 231-44.
Fabregas, J., Herrero, C., Abalde, J. & Cabezas, B. (1985b). Growth, chloro-
phyll a and protein of the marine microalga Isochrysis galbana in batch
cultures with different salinities and high nutrient concentrations. Aquacul-
ture, 50, 1-11.
Gonzalez-Rodriguez, E. & Maestfini, S. Y. (1984). The use of some agricultural
fertilizers for the mass production of marine algae. Aquaculture, 36, 245-56.
Hoeffner, S. L. & Manahan, S. E. (1980). Influence of coal humic acid on the
growth of ChloreUa vulgaris algae. J. Env. Sci. Health, Part A, 15(2), 149-61.
Laing, I. & Utting, S. D. (1980). The influence of salinity on the production of
two commercially important unicellular marine .algae. Aquaculture, 21,
79-86.
Parsons, T. R. & Strickland, J. D. H. (1965). Particulate organic matter. III.I.
Pigment analysis. III.II. Determination of phytoplankton pigments. J. Fish.
Res. Bd. Can., 18, 117-27.
Persoone, G. & Claus, C. (1980). Mass culture of algae: a bottleneck in the
nursery culturing of molluscs. In: Algae Biomass: Production and Use, ed. G.
Shelef and C. J. Soeder, Elsevier/North Holland Biomedical Press, Amster-
dam, pp. 265-85.
Prakash, A. & Rashid, M. A. (1968). Influence of humic substances on the
growth of the marine phytoplankton: dinoflagellates. Limnol. Oceanogr., 13,
598-606.
Prakash, A., Rashid, M. A., Jensen, A. & Rao, D. (1973)..Influence of humic
substances on the growth of marine phytoplankton: diatoms. Limnol.
Oceanogr., 18, 516-24.
Shelef, G. & Soeder, C. J. (eds) (1980). Algae Biomass: Production and Use.
Elsevier/North Holland Biomedical Press, Amsterdam.
Soeder, C. J. (1980). Massive cultivation of microalgae: results and prospects.
Hydrobiologia, 72, 197-209.
Stein, J. R. (ed.) (1973). Handbook of Phycological Methods: Culture Methods
and Growth Measurements. Cambridge University Press, Cambridge.
Ukeles, S. R. (1980). American experience in the mass culture of microalgae for
feeding larvae of the American oyster, Crassostrea virginica. In: Algae
Biomass: Production and Use, ed. G. Shelef and C. J. Soeder, Elsevier/North
Holland Biomedical Press, Amsterdam, pp. 287-306.
Walne, E R. (1974). Culture of Bivalve Mollusc. 50 Years' Experience at Conwy.
Fishing News (Books), Farnham.