A.N.U. M.

PHARMACY SYLLABUS (WITH EFFECT FROM 2012 - 13 ACADEMIC YEAR)

MPH 101 (T)

COMMON PAPER (FIRST SEMESTER)
(ALL BRANCHES)

I/II M.PHARMACY
ADVANCED INSTRUMENTAL METHODS OF ANALYSIS (THEORY)

Unit : 1 : UV.VISIBLE SPECTROSCOPY :

Brief review of electromagnetic spectrum and absorption of radiations.
The chromophore concept, absorption law and limitations. Theory of elec-
tronic spectroscopy, absorption by organic molecules, choice of solvent
and solvent effects, modern instrumentation - design and working princi-
ple. Applications of UV-Visible spectroscopy (qualitative and quantitative
analysis), Woodward - Fischer rules for calculating absorption maximum.
Unit : 2 : INFRARED SPECTROPHOTOMETRY ;
Introduction, basic principles, vibrational frequency and factors influ-
encing vibrational frequency, instrumentation and sampling techniques,
interpretation of spectra, applications in Pharmacy. FT-IR-theory and ap-
plications, Attenuated Total Reflectance (ATR).
Unit : 3 : NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY :
Fundamental Principles and Theory, Instrumentation, solvents, chemical
shift and factors affecting chemical shift, spin-spin coupling, coupling con-
stant and factors influencing the value of coupling constant, spin-spin
decoupling, proton exchange reactions, FT-NMR, 2D-NMR, NMDR, NOE,
NOESY, COSY and applications in Pharmacy, interpretation of spectra, C13
NMR-Introduction, Natural abundance, C13 NMR Spectra and its struc-
tural applications.
Unit : 4 : MASS SPECTROSCOPY:
Basic principles and instrumentation, ion formation and types, frag-
mentation processes and fragmentation pattern, Chemical ionization mass
spectroscopy (CIMS), Field Ionization Mass Spectrometry (FIMS), Fast Atom
Bombardment MS(FAB MS), Matrix Assisted laser desorption/ionization MS
(MALDI-MS), interpretation of spectra and applications in Pharmacy.
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A.N.U. M.PHARMACY SYLLABUS (WITH EFFECT FROM 2012 - 13 ACADEMIC YEAR)

Unit : 5 : CHROMATOGRAPHIC TECHNIQUES :

a) Classification of chromatographic methods based on mechanism
of separation; paper chromatography, thin layer chromatography, ion ex-
change chromatography, column chromatography and affinity chromatog-
raphy - techniques and applications.
b) Gas Chromatography : Theory and principle, column operation,
instrumentation, derivatisation methods and applications in Pharmacy.
c) High Performance Liquid Chromatography : Principle, instrumen-
tation, solvents used, elution techniques, RP-HPLC and applications in phar-
macy.
d) HPTLC : Theory and Principle, instrumentation, elution techniques
and pharmaceutical applications.
Unit : 6 : X-RAY DIFFRACTION AND DSC, DTA METHODS :
Introduction, Generation of X-Rays, Elementary crystallography; miller In-
dices, X-ray, diffraction Braggs law, X-ray powder diffraction, X-ray powder
diffractometer, obtaining and interpretation of X-ray powder diffraction data.
Applications of XRD, DSC and DTA in the characterization of Pharmaceuti-
cal solids.
Unit : 7 : STATISTICAL ANALYSIS :
Introduction, significance of statistical methods, normal distribution,
probability, degree of freedom, standard deviation, correlation, variance,
accuracy, precision, classification of errors, reliability of results,
confidence interval. Test for statistical significance - students T-test,
F-test, Chi-square test, correlation and regression.

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A.N.U. M.PHARMACY SYLLABUS (WITH EFFECT FROM 2012 - 13 ACADEMIC YEAR)

MPH 102 (P)

I/II M.PHARMACY (1st SEMESTER)
ADVANCED INSTRUMENTAL METHODS OF ANALYSIS (PRACTICALS)
01. Determination of Isobestic point using bromocresol green.
*02. Estimation of ranitidine hydro chloride in pharmaceutical formulation
by PDAB reagent.
*03. Estimation of aceclofenac in pharmaceutical formulation by FC
reagent.
04. Estimation of ciprofloxacine in pharmaceutical formulation by ferric.
*05. Estimation of sulphamethoxazole in pharmaceutical formulation by BM
reagent.
06. Estimation of paracetamol (or) gatifloxacine (or) frusemide in
pharmaceutical formulation by UV spectroscopy.
07. Simultaneous estimation of amoxicillin & dicloxacillin (or) paracetamol
& nimesulide in pharmaceutical formulation by UV spectroscopy.
08. Estimation of Vit B1 & metformin (or) atorvastatin & ezetimib in
pharmaceutical formulation by absorbance ratio method.
09. Estimation of paracetamol & diclofenac (or) ornidazole & ofloxacine in
pharmaceutical formulation by derivative spectroscopy.
10. Estimation of moisture content by KFR
11. Estimation of sodium ions by flame photometer.
12. Estimation of potassium ionsby flame photometer
*13. Estimation of paracetamol (or) aspirin (or) nimesulide in
pharmaceutical formulation by HPLC.
*14. Simultaneous estimation of paracetamol & nimesulide in
pharmaceutical formulation by HPLC.
*15. Estimation of paracetamol in pharmaceutical formulation by using
caffeine as internal standard by HPLC.
16. Characterization of pharmaceutical solids by using recorded data
of XRD and DSC.
17. Prediction of -max for different organic compounds by using
woodward fischer rule.
18. Interpretation of IR spectras for different organic compounds.
19. Interpretation of NMR spectras for different organic compounds.
20. Interpretation of MASS spectras for different organic compounds.
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A.N.U. M.PHARMACY SYLLABUS (WITH EFFECT FROM 2012 - 13 ACADEMIC YEAR)

REFERENCE BOOKS :
01. Spectrometric identification of Organic Compounds,
Robert, M.Silverstein et al, 7th edition, 1981.
02. Fundamentals of Mathematical Statistics, S.C.Gupta and V.K.Kapoor.
03. Principles of Instrumental Analysis by Donglas A.Skoog, James, J.Leary,
4th Edition.
04. Pharmaceutical Analysis - Modern Methods - Part A, Part B, James W.
Munson - 2001.
05. Vogels Text Book of Quantitative Chemical Analysis, 6th Edition, 2004.
06. Chromatographic Analysis of Pharmaceuticals, John A.Adamovics, 2nd
Edition.
07. Practical Pharmaceutical Chemistry, Part two, A.H.Beckett
& J.B.Stenlake - 4th Edition.
08. Instrumental Methods of Chemical Analysis - B.K.Sharma - 9th
Edition.
09. Instrumental Methods ofAnalysis - Hobert H.Willard, 7th Edition.
10. Organic Spectroscopy - William Kemp, 3rd Edition.
11. Techniques and Practice of Chromatography - Raymond P.W.Scott,
Vol. 70.
12. Identification of Drugs and Pharmaceutical Formulations by Thin Layer
Chromatography - P.D.Sethi, Dilip Charegaonkar, 2nd Edition.
13. HPTLC-Quantitative Analysis of Pharmaceutical Formulations -
P.D.Sethi,
14. Liquid Chromatography - Mass Spectrometry, W.M.A.Niessen, J.Van
Der Greef, Vol. 58.
15. Stereo Chemistry - Conformation and Mechanism by P.S.Kalsi,
2nd Edition.
16. Spectroscopy of Organic Compounds by P.S.Kalsi.
17. Organic Chemistry by I.L.Finar Vol. II - 5th Edition.

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A.N.U. M.PHARMACY SYLLABUS (WITH EFFECT FROM 2012 - 13 ACADEMIC YEAR)

MPH 101 (T)

I/II M.PHARMACY (1ST SEMESTER)
ADVANCED INSTRUMENTAL METHODS OF ANALYSIS (THEORY)
MODEL QUESTION PAPER
TIME: 3HOURS MAX MARKS: 70
ALL QUESTIONS CARRY EQUAL MARKS
ANSWER ANY FIVE QUESTIONS
1. (a) Explain Beer-Lamberts law and discuss about the deviations from
beers law.
(b) What are the most frequent electronic transitions during
absorption of electromagnetic Radiation? Which results is more
intense absorption? Give examples of compounds that exhibit
each.
2. (a) Explain the different detectors used in gas chromatography with
a neat diagram.
(b) Distinguish between packed columns and capillary columns in
GC. Add note on Van deemter equation.
3. Explain the following terms:
a) Reverse-phase chromatography.
b) Normal phase chromatography.
c) Column efficiency parameters.
4. a) Explain the term chemical shift and discuss the factors
influencing chemical shift.
b) Write a short note on the following:
(i) Longitudinal relaxation (ii) Transverse relaxation
5. (a) What is the necessary criterion for absorption to occur in the IR
region? What types of molecular vibration are associated with IR
absorption?
(b) Name the functional groups that are present in a molecule for
the corresponding wave numbers in an IR spectrum.
(i) 3300 cm-1 (ii) 1690 cm-1
(iii) Band at 1600, 1490 and 850-700cm-1
(iv) 3000 cm-1
6. a) Explain the principal involved in FAB-Mass spectrometry with
example.
b) Describe briefly the instrumentation of a quadruple mass
spectrometer and write its advantage.
7. a) Write the principle involved and working of X-ray power diffracto
meter. Add note on Application of X-ray diffractometer in
pharmacy with example.
b) Explain with example how DSC and DTA studies are useful in
preformulation and formulation Development studies.
c) C13 NMR
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A.N.U. M.PHARMACY SYLLABUS (WITH EFFECT FROM 2012 - 13 ACADEMIC YEAR)

MPH 102 (P)

I/II M.PHARMACY (1ST SEMESTER)
MODEL QUESTION PAPER (PRACTICALS)
Time: 6 hrs Max Marks: 70
1. Synopsis : 15 Marks
*2. Major Experiment : 25 Marks
3. Minor Experiment : 15 Marks
4. Viva-voce : 15 Marks

Note : Total sessional marks is 30 (20 for practical sessional plus 10 marks
for regularity, promptness, viva-voce and record maintenance).

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