1.

Which one or more of the following equations accurately reflects
DNA base pairing?

2. Why must the genetic code be triplet and not doublet?

3. The gene for one of the polypeptide chains in hemoglobin
consists of 438 bases. How many amino acids are in the
polypeptide?

4. Give two similarities between transcription and DNA replication.

5. Give two differences between translation and DNA replication.

6. Give two differences between transcription and translation.

Page 1 of 4

The work of Hershey and Chase (1952). Allow phages to Allow phages to complete complete life cycle life cycle inside bacteria inside bacteria 5. The phage has a simple structure consisting only of DNA with a protein coat. If a mutation changes one base. 8. suggest: a) Why a non-overlapping code is better than an overlapping one. Evidence that DNA is the genetic material. b) Why a degenerate code is better than a non-degenerate code. this substance would be the genetic material. Mutations can change DNA bases causing a different amino acid to be coded for. Whichever substance entered was able to control the activities of the bacterial cell and instruct it to produce new phages. Was it the protein or was it the DNA? The results are shown in the below table. Steps Experiment 1 Experiment 2 1. Phages infect bacteria Phages infect bacteria 3. Read the passage and answer the questions at the end. Bacteria do not become Bacteria become radioactive radioactive 4.coli. Prepare phages with Prepare phages with radioactively labeled radioactively labeled sulphur phosphorus 2. Page 2 of 4 .7. i.e. This experiment was designed to prove whether it was the protein or the DNA from the phage that entered the bacterium. Part of the phage enters the bacterium and causes the bacterium to produce new phages. The new generation of The new generation of phages released from phages released from bacteria contain no bacteria do contain radioactivity radioactivity. A phage is a virus that is a parasite of bacteria such as E.

a) Below is a diagram of a section of mRNA showing the sequence of three of the codons. Page 3 of 4 . why were some of the new generations of phages are radioactive? 9. a) Which molecules in the phage were being labeled by: i) Radioactive sulphur ii) Radioactive phosphorus b) Why was radioactive carbon not used to label the phage? c) Which part of the phage enters the bacteria and what is the evidence? d) In experiment 2.

Explain the consequence of a mutation which deletes the U from codon2. ii) The DNA sequence which coded for codon3.State the base sequence of: i) The tRNA anticodon complementary to codon1. Page 4 of 4 . b) The three codons in the above figure are near the start of the sequence coding for a protein. c) Describe the role of the ribosome in translation.

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