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Spectrophotometer set-up instructions for bacterial/BPB

measurements

Press button. Screen should look like this:

Leave default setting of 1 and hit enter.


You will be prompted to type in a .




For both parts B and D they will use a of 590nm.
Punch those values in using the keypad and hit enter.




Leave the default setting of NO and hit enter.




They can now place their blank in the spec. and hit Read Blank.
They will obviously obtain an absorbance reading of 0 and they
will be prompted to hit the right-pointing arrow key to continue.
They now just have to hit Read Sample to collect any other
readings.
*Remind them that if they switch between parts B and D they
must re-blank with the appropriate solution.

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