Description

Designation:NRS231
DepositedName:Bacillussubtilis(Ehrenberg)Cohn
ProductSheet ProductDescription:Thisitemisusedinsterilitytestingandassayofrifampin[rifampicin,rifamycinAMP],
neomycin,monensin,kanamycin,lasalocid[X537A],mitomycin[mitomycinC],vancomycin,novobiocin,
Bacillussubtilissubsp. penicillin,framycetin,tobramycin,spiramycin,gentamicins[gentamicin],amoxicillin,bekanamycin[kanamycinB

spizizenii(ATCC6633) aminodeoxykanamycin],hygromycinB,streptomycin,erythromycin,dactinomycin[actinomycinactinomycin
D],streptonigrin,lactamantibiotics[betalactamantibiotics],capreomycinsulfate[capreomycin],and
dihydrostreptomycinsulfate[dihydrostreptomycin].Susceptibilitydisctestingofrifampin[rifampicinrifamycin
AMP]andnovobiocin.ProducesrestrictionendonucleaseBsu6633Iandsubtilin.
PleasereadthisFIRST
Propagation
StorageTemp.
Frozen:80Cor Medium
colder ATCCMedium44:BrainHeartInfusionAgar/Broth

FreezeDried:2C GrowthConditions
to8C Temperature:30C
LiveCulture:See Atmosphere:Aerobic
Propagation
PropagationProcedure
Section 1. Openvialaccordingtoenclosedinstructions.
2. Usingasingletubeof#44broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror
1.0mLpipette.Rehydratetheentirepellet.
3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.

BiosafetyLevel
4. Useseveraldropsofthesuspensiontoinoculatea#44agarslantand/orplate.
1 5. Incubatethetubesandplateat30Cfor24hours.

IntendedUse Notes

Thisproductisintendedforresearchuseonly.Itisnot Colonieson#44agararecircular,undulate,matte,slightlypeaked,opaque,andoffwhite.
intendedforanyanimalorhumantherapeuticor
diagnosticuse. AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.

CitationofStrain References

Ifuseofthiscultureresultsinascientificpublication,it
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.
shouldbecitedinthatmanuscriptinthefollowing
manner:Bacillussubtilissubsp.spizizenii
BiosafetyLevel:1
(ATCC6633)

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty

ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein
mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
longervalid.
AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA Disclaimers
www.atcc.org

Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
800.638.6597or703.365.2700
Fax:703.365.2750
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
Email:Tech@atcc.org ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
Orcontactyourlocaldistributor
confirmedtobeaccurate.

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 Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom
themisidentificationormisrepresentationofsuchmaterials.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org
ProductSheet
Bacillussubtilissubsp. AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2017.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[01/12]
spizizenii(ATCC6633)

PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder

FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

IntendedUse

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain

Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Bacillussubtilissubsp.spizizenii
(ATCC6633)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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