Use of Micropipettor and

Spectrophotometer:
Testing Accuracy of Operators (Weight)
GROUP 8
Calagui
Salaysay
So
Urbano
Yap
Testing Accuracy of Operators- Weight

Accuracy
the closeness of the measured value to the true value
percent error is often used
Testing Accuracy of Operators- Weight
The standard value is derived using the density of water, 0.9977g/mL at 22C.
Testing Accuracy of Operators- Weight
Computing for Percent error:
 Table 1.1 Weight of different water samples by 1
member of the group (Group 1)
50 L 100 L 500 L
Student
Wt Deviation % Error Wt Deviation % Error Wt Deviation % Error

1 0.0499 1x10-4 0.2 0.1001 1x10-4 0.1 0.5016 1.6x10-3 0.32

2 0.0495 5x10-4 1 0.1000 0 0 0.5020 2x10-3 0.4

3 0.0495 5x10-4 1 0.1001 1x10-4 0.1 0.5023 2.3x10-3 0.46

4 0.0499 1x10-4 0.2 0.1002 2x10-4 0.2 0.5016 1.6x10-3 0.32

Ave 0.0497 3x10-4 0.6 0.1001 1x10-4 0.1 0.5019 1.875x10-3 0.375

Stddev 2.309x10-4 8.1650x10-5 3.4034x10-4

 Table 1.1 Weight of different water samples by 1
member of the group (Group 3)
50 L 100 L 500 L
Student
Wt Deviation % Error Wt Deviation % Error Wt Deviation % Error

1 0.0510 1x10-3 2 0.0992 8x10-4 0.8 0.5035 3.5x10-3 0.70

2 0.0500 0 0 0.0993 7x10-4 0.7 0.5043 4.3x10-3 0.86

3 0.0500 0 0 0.0988 1.2x10-3 1.2 0.5032 3.2x10-3 0.64

4 0.0490 1x10-3 2 0.0999 1x10-4 0.1 0.5051 5.1x10-3 1.02

Ave 0.0500 5x10-4 1 0.0993 7x10-4 0.7 0.50425 4.025x10-3 0.805

Stddev 8.2x10-4 4.5x10-4 8.5x10-4

 Table 1.4 Weight of different water samples by 1
member of the group (Group 5)
50 L 100 L 500 L
Student
Wt Deviation % Error Wt Deviation % Error Wt Deviation % Error

1 0.0506 6x10-3 1.2 0.1009 9x10-4 0.9 0.5023 2.3x10-3 0.46

2 0.0500 0 0 0.1006 6x10-4 0.6 0.5012 1.2x10-3 0.24

3 0.0506 6x10-4 1.2 0.0999 1x10-4 0.1 0.5006 6x10-3 0.12

4 0.0500 0 0 0.1002 2x10-4 0.2 0.5040 4x10-3 0.8

Ave 0.0503 6x10-4 1.2 0.1004 4.5x10-4 0.45 0.5020 2.025x10-3 0.405

Stddev 3.46x10-4 4.4x10-4 1.49x10-3

 Table 1.4 Weight of different water samples by 1
member of the group (Group 7)
50 L 100 L 500 L
Student
Wt Deviation % Error Wt Deviation % Error Wt Deviation % Error

1 0.0430 6.92025x10-3 13.86 0.1065 6.6595x10-3 6.67 0.5022 2.9975x10-3 0.60

2 0.0498 1.2025x10-7 0.24 0.1000 1.595x10-4 0.16 0.5045 5.2975x10-3 1.06

3 0.0503 3.7975x10-5 0.76 0.1000 1.595x10-4 0.16 0.5051 3.8475x10-3 0.75

4 0.0500 7.975x10-5 0.16 0.1049 5.0595x10-3 5.07 0.5062 6.9975x10-3 1.40

5 0.0504 4.7975x10-4 0.96 0.1000 1.595x10-4 0.16 0.5047 5.9975x10-3 1.10

Ave 0.0487 1.5036x10-3 3.196 0.10228 2.4395x-3 2.44 0.50414 5.0275x10-3 0.982

Stddev 3.20x10-5 3.17x10-3 1.54x10-3

 Table 1.6 Compiled results of all groups for procedure 2
Grp. 50L 100L 500L

Wt Deviation %Error Std. Error Wt Deviation %Error Std. Wt Deviation %Error Std.
Error Error

1 0.4970 3x10-4 0.6 1.15x10-4 0.1001 1x10-4 0.1 4.08x10-4 0.5019 1.875x10-3 0.375 1.7x10
-4

3 0.0500 0.0005 1.0 4.1x10-4 0.0993 0.0007 0.7 4.75x10-4 0.50425 0.004025 0.805 4.25x1
0-4

5 0.0503 6x10-4 1.2 1.73x10-4 0.1004 4.5x10-4 0.45 2.2x10-4 0.5020 2.025x10-3 0.405 7.45x1
0-5

7 0.0487 1.5036x10-3 3.196 1.43x10-5 0.10228 2.4395x-3 2.44 1.42x10-3 0.50414 5.0275x10-3 0.982 6.89x1
0-4

Standard values: 0.0499g for 50L, 0.0997g, for 100L, 0.4989g for 500L
Testing Accuracy of Operators- Weight
Computing for Standard Error:
Possible sources of Error:
Disregarding Temperature
Pipetting at an Angle
Tip Wiping
Failure to Pre-Wet Pipette Tip
Poor pipetting skills
Choosing the wrong micropipette
Uncalibrated analytical balance
Conclusion
The accuracy of the micropipettor refers to its performance relative to an accepted value. It is
computed by solving for the percent error between the experimentally measured volume of the
solution and the volume that is expected. The accuracy of micropipettors lessens when they are
set to measure volumes closest to the lowest values in their range. Possible sources of error
include disregarding temperature, pipetting at an angle, tip wiping, failure to pre-wet pipette tip,
poor pipetting skills, and choosing the wrong micropipette.