Title: Leukocyte observation Name: Do Le Tan

Date: 20/04/2011 Lab Partners:

Introduction:
In the nature, almost the cell in side is transparent and need to be dyed to
observe. Cytochemistry is the method used for the chemical nature of the cell, and it
will be used to observer many kind of cells in our blood such as:

Red Blood Cells :

Erythrocytes,or red blood cells,are biconcave disks.They transport the
oxygen used in aerobic respiration and carry away some carbon dioxide
wastes.When oxygen diffuses into blood,it binds with hemoglobin,an iron-
containing pigment that gives red blood cells their colour.

White Blood Cells

Leucocytes,or white blood cells,function in daily housekeeping or

defense.They differ in size,nuclear shape and staining traits.There are five
types: neutrophils, eosinophils, basophils, monocytes and lymphocytes. In this
experiment, only monocytes and lymphocyte are observed

Monocytes: are large mononuclear cells.This nucleus is quite large,sometimes

round,but more usually irregular.The monocytes differ from lymphocytes in that
they can spread on a glass surface.

Lymphocytes: nucleus is generally centrally placed and round but it may be

oval or slightly concave.The cytoplasm is basophilic and shows mitochondria,together
with a few azurophile granulations and a centrosome which is particularly clearly
defined.

Materials:
1. Drops of animal blood (except poultry)
2. Slides
3. Giemsa stain solution
4. Ethanol
5. Water
Methods:
1. Smear specimen preparation.
2. Drop a small drop of blood on the slide and spread it lightly and rapidly.
3. Air dry.
4. Fix slide in ethanol for 5 minutes.
5. Air dry.
6. Place Giemsa stains solution for 2 minutes; try to shake it gently during
incubation.
7. Rinse slide with water.
8. Air dry.

Data:
Image 1:
There is 1 lymphocyte on the right which has pink color and has the nucleus
inside fill nearly all the cell.
There is 1 monocyte on the left which has also pink color by giemsa but the
cell has nucleus inside does not fill cell

Image 2:
There is another image of monocyte which is the pink one

Discussion:
The blood smear should be as thin as possible to be able to observe cells
clearly and not to have too much of them.
 Tap water will not contaminate the blood because before rinsing with water it is
fixed. By fixation treatment, the general morphology and internal structures are
preserved.

Write the name of the experiment on the slide after dropping blood and spread
it to avoid negligence

Control slide is also used in this experiment. It is better to set paper under the
slide when place giemsa solution to the slide

Conclusion: