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10-5-17
PCR Lab Report
Purpose: The purpose of this lab was to take DNA from our mouths replicate DNA
segments by using PCR. Then, we wanted to sequence our DNA by using gel
electrophoresis. By doing this, we hoped to learn more about where our DNA came
from. Commented [1]: More concise
Hypothesis: If we successfully followed the procedure and the steps correctly, then we
will have replicated and sequenced DNA segments that can tell us where I came from
and if other people came from similar backgrounds. Commented [2]: What is your expected result?
Procedure: The procedure goes as following, Commented [3]: Cite the packet next time
Analysis: The data was inconclusive for my experiment, so I cannot analyze the data.
This same thing happened to many other students in our class, so we made a fake set
for the class to analyze and try to find alu repeats within the set. We then found the
frequencies of different alleles (+/+, +/-, -/-) within the class. If you have a +/+ that
means that you have an alu repeat. If you have a +/-, then you have an alu insert in
only one chromosome and -/- means that you have no alu inserts.
After finding the results with the class set, I found my hypothesis to be correct. If Commented [4]: if you got no data how can you say
correct?
I was to get a correct reading of my DNA and my alleles, I would have been able to
trace my background and compare that with what others found.
Since this was our first experiment, I think that there were some issues with the
lab that could be fixed. First off, we probably didnt get the correct measurements and
amounts that we needed to get. Also, some of the loading dye didnt go into the wells
but instead went into the gel which may have messed up the electrophoresis process. Commented [5]: are these the errors? any more?
We could improve this lab by having a way to measure exactly how much we were
putting in or taking out of the liquids. Another way we could improve this lab would be
to have someone put the loading dye into the wells for us. This would help us because
it would be more dependable than students putting in the loading dye. Commented [6]: Improvements have to be procedural.