University of Central Punjab

Final Term Examination

2nd Semester, F-16 (summer)
Paper: Pharmaceutical Biochemistry-II
Name: ____________ Total Marks: 30
Roll No: ____________ (SUBJECTIVE) Time: 70 Minutes

Attempt any five questions.

Q.2
a) Explain the biosynthesis of Palmitic acid by drawing a flow chart. (06 marks)

Q.3
How is cholesterol synthesized? Explain by drawing a flow chart, also write the
functions of cholesterol. (06 marks)

Q.4
a) Explain biosynthesis of Heme by drawing a flow chart. (06 marks)

Q.5
Explain beta oxidation of fatty acids. Also discuss the energetics of Stearic acid.
(06 marks)

Q.6
Explain urea cycle and also discuss its significance.
(06 marks)

Q.7 Write note on any two.

a) De Novo purine synthesis (03 marks)

b) De Novo Pyrimidine Synthesis (03 marks)

c) Purine catabolism (03 marks)

 University of Central Punjab
Final Term Examination
2nd Semester, F-16 (summer)
Paper: Pharmaceutical Biochemistry-II
Name: _________________ Total Marks: 20
Roll No: _________________ (OBJECTIVE) Time: 20 Minutes

Q.1: Attempt all parts. Each carries 01 mark. Over writing or cutting is not allowed.

Encircle the best possible choice

1. Which of the following intermediate in cholesterol synthesis is required for making Coenzyme
Q?
(A) HMG CoA
(B) Lanosterol
(C) Squalene
(D) Farnesyl PPi
2. The main site of urea synthesis in mammals is;
(A) Liver
(B) Skin
(C) Intestine
(D) Kidney
3. Which of the following drugs is the inhibitor of Thymidyalate Synthetase?
(A) 6-Mercaptopurine
(B) 5-Fluorouracil
(C) Methotrexate
(D) Trimethoprim
4. The enzymes of urea synthesis are found in;
(A) Mitochondria only
(B) Cytosol only
(C) Both mitochondria and cytosol
(D) Nucleus
5. Which of the following is NOT the function of Heme?
(A) It is necessary for the synthesis of Hemoglobin
(B) It is necessary for the synthesis of cytochromes
(C) It is an integral component of Purines
(D) It is necessary for the synthesis of catalase enzyme
6. The dietary fats are transported as;
(A) Micelles
(B) Chylomicrons
(C) Fatty acid Albumin complex
(D) Liposomes
7. NADPH required for the nucleotide synthesis can be generated from;
(A) HMP pathway (B) Glycolysis
(C) TCA cycle (D) All of the above
8. The key regulatory enzyme of cholesterol synthesis is?
(A) HMG Co A synthetase
(B) HMG Co A carboxylase
(C) HMG CoA reductase
(D) Thioesterase
9. Which out of the followings is NOT a ketone body?
(A) Acetone (B) Acetoacetate
(C) Beta-hydroxy butyrate (D) Hydroxymethyl Glutarate
10. Dihydrofolic acid reductase is inhibited by;
(A) 5-Fluorouracil
(B) 6-Mercaptopurine
(C) Allopurinol
(D) Methotrexate
11. In eukaryotes, fatty acid oxidation occurs in;
(A) Mitochondrial matrix (B) cytosol
(C) Cell membrane (D) endoplasmic reticulum
12. Which of the following amino acid is NOT required for the synthesis of purines?
(A) Glutamate (B) Alanine
(C) Glycine (D) Aspartate
13. How many ATPs are formed during complete oxidation of palmitate?
(A) 35 (B) 96
(C) 129 (D) 131
14. Ketone body synthesis occur in;
(A) Muscles (B) Liver
(C) Adipose tissue (D) RBCs
15. Which of the following is NOT a bile acid?
(A) Cholic acid
(B) Deoxycholic acid
(D) Chenodeoxycholic acid
(E) Arachidonic acid

Q 2 Encircle T in case of true statement and F in case of false statement

16. Bilirubin is lipid soluble and is transported in the blood attached to serum albumin. T / F

17. Severe Combined Immunodeficiency Syndrome is caused by the deficiency of HGPRT T / F

18. Pyrimidines are synthesized de novo in the cytoplasm from aspartate, C02, and glutamine. T / F

19. Activation of hormone sensitive lipase is important for lipolysis. T / F

20. Beta oxidation of fatty acids occurs in all body cells containing mitochondria T / F