7.75758.0003-xxxxxxxxxx msp.

November 2013
Notes on the measurement:
1.01787.0001
Wipe,
For photometric measurement the cells must be clean.
if necessary, with a clean dry cloth.
Spectroquant Turbidities in the lower phase (green organic phase) yield false-high readings.
Surfactants (nonionic) after
The color of the measurement solution remains stable for at least 60 min
the end of the reaction time stated above.
Cell Test
8. Analytical quality assurance
1. Method recommended before each measurement series
To check the photometric measurement system (test reagents, measurement
Nonionic surfactants (ethoxylates with 3 to 20 ether bridges) react with an indi-
device, handling) and the mode of working, a freshly prepared standard solution
cator (TBPE) to form a complex that is then extracted with dichloromethane. The
containing 4.00 mg/l Triton X-100 (application see the website) can be used.
green color of the organic phase is determined photometrically.
Sample-dependent interferences (matrix effects) can be determined by means
of standard addition.
2. Measuring range and number of determinations Additional notes see under www.qa-test-kits.com.

Measuring range Number of determinations Characteristic quality data:

In the production control, the following data were determined in accordance with
0.10 - 7.50 mg/l 1) 25 ISO 8466-1 and DIN 38402 A51:
1)
calculated as Triton X-100 Standard deviation of the method + 0.059
For programming data for selected photometers / spectrophotometers see (mg/l Triton X-100)
www.service-test-kits.com. Coefficient of variation of the method + 1.5
(%)
Confidence interval + 0.14
3. Applications (mg/l Triton X-100)
This test measures dissolved nonionic surfactants (ethoxylates with 3 to 20 Number of lots 16
ether bridges).
Sample material: Characteristic data of the procedure:
Surface water
Sensitivity: 0.06
Drinking water Absorbance 0.010 A
Wastewater corresponds to (mg/l Triton X-100)
Degreasing baths Accuracy of a measurement value max. 0.26
Wash solutions (mg/l Triton X-100)
Process water
This test is not suited for seawater. For quality and batch certificates for Spectroquant test kits see the website.

4. Influence of foreign substances 9. Note

This was checked in solutions containing 4 and 0 mg/l Triton X-100. The deter- The test reagents must not be run off with the wastewater!
mination is not yet interfered with up to the concentrations of foreign substances Information on disposal can be obtained at www.disposal-test-kits.com.
given in the table.
Concentrations of foreign substances in mg/l or %
3+
Al 200 NH4+ 500 Anionic surfactants 100
Ca2+ 500 Ni2+ 200 Cationic surfactants 1
Cu2+ 100 NO2- 500 NaCl 2%
Fe3+ 100 Zn2+ 100 NaNO3 5%
Mg2+ 200 Na2SO4 2%

5. Reagents and auxiliaries

Please note the warnings on the packaging materials!
The test reagents are stable up to the date stated on the pack when stored
closed at +15 to +25 C.

Package contents:
25 reaction cells
1 sheet of round stickers for numbering the cells
Other reagents and accessories:
MColorpHastTM Universal indicator strips pH 0 - 14, Cat. No. 109535
Sodium hydroxide solution 1 mol/l TitriPUR, Cat. No. 109137
Sulfuric acid 0.5 mol/l TitriPUR, Cat. No. 109072
Triton X-100, Cat. No. 112298
Hydrochloric acid 25 % for analysis EMSURE, Cat. No. 100316
Methanol for analysis EMSURE, Cat. No. 106009
Pipette for a pipetting volume of 4.0 ml

6. Preparation
leave
The glassware must be free from surfactants! It is thus recommended to
it to stand filled with methanolic hydrochloric acid (make up 11 ml of
hydrochloric acid 25 % to 100 ml with methanol) for several hours and subse-
quently rinse it thoroughly with distilled water.
Analyze immediately after sampling.
The pH must be within the range 3 - 9.
Adjust, if necessary, with sodium hydroxide solution or sulfuric acid.

7. Procedure
Pretreated sample 4.0 ml Pipette into a reaction cell (20 - 25 C), close the
(20 - 25 C) cell tightly, and shake vigorously for 1 min.

Leave to stand for 2 min (reaction time).

Swirl the cell before the measurement.
Measure the sample in the photometer. Merck KGaA, 64271 Darmstadt, Germany,
Tel. +49(0)6151 72-2440
www.analytical-test-kits.com
EMD Millipore Corporation, 290 Concord Road,
Billerica, MA 01821, USA, Tel. +1-978-715-1335