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NAME: _______________________________________________

MATRIC NO: ____________________________



Degree of BSc. In Biochemical-Biotechnology Engineering


Mid semester Exam

Semester 2, 2016/2017

(BTE 2440)

Time allowed 1 hour

Answer ALL questions.

SECTION A: True or False question. CIRCLE the right answer.

Q1. [1 marks]
Quorum sensing in microbial population involves secretion and detection of
chemical signals by the organisms.
True or false

Q2. [1 marks]
Barophilic organisms require or grow more rapidly in the presence of increased
True or false

Q3. [1 marks]
Microaerophile microorganisms lack catalase and superoxide dismutase (SOD).
True or false

Q4. [1 marks]
Electronic counters and spectrophotometer can be used to measure of viable cell
True or false

Q5. [1 marks]
Chemostat operates best at low dilution rate.
True or false

Q6. [1 marks]
Robert Koch developed the process of steam sterilization technique.
True or false

Q7. [1 marks]
Edward Jenner used a vaccination procedure to protect individuals from smallpox.
True or false

Q8. [1 marks]
Robert Koch pioneered usage of solid nutrient media
True or false

Q9. [1 marks]
Possible reason for entry into stationary phase is due to accumulation of resistant
True or false
Q10. [1 marks]
Cells adapt themselves to growth conditions and synthesizing new components
during log phase.
True or false

SECTION B: Write your answers based on the instruction of each question.

Q11. [10 marks]

Filling in the blanks with appropriate answers given below on the following

facilitated diffusion, passive diffusion, active transport, group translocation

a. Requires ABC transporters for movement of substance.


b. Molecules are modified as they are transported across the membrane.


c. Moves molecules against the gradient. _____________________

d. Movement of molecules is not energy dependent and requires protein carrier.


e. H2O and CO2 often move across membranes this way. __________________

Q12. [10 marks]

Filling in the blanks with appropriate answers given below on the following

acidophiles, endospores, facultative anaerobes, Microaerophiles, Aerotolerant


a. Ignore O2 and grow equally well whether it is present or not.


b. Microorganism growth optimum between pH 0 and pH 5.5.


c. __________________ contain SOD and catalase which destroy superoxide

radical and H2O2, respectively.

d. __________________ survive large dose of ionizing radiation.

e. Microorganisms that requires lower levels (2 to 10%) of oxygen for growth.

SECTION C: Answer BOTH questions.

Q13. [10 marks]

a. What are the differences between prokaryotes and eukaryotes? (4 marks)

b. Eubacteria and Archaebacteria are both prokaryotes. What are the unique
characteristics of Archaebacteria that differentiate them from Eubacteria?
(2 marks)

c. Give examples of microorganism that is categorized under Eubacteria. (2 marks)

d. Aspergillus and Amoeba are both eukaryotes. Aspergillus is categorized under

fungi while Amoeba falls under Protista. Give possible explanations what makes
Aspergillus and Amoeba falls under different Kingdom. (2 marks)
Q14. [10 marks]
A) Below is the branched pathway of electron transport chain (ETC) for Escherichia
coli. Questions (a) - (c) are related to the diagram below.

a) Explain in detail what happen when electron from NADH is transported

through the electron transport chain above to the final electron acceptor,
oxygen? (3 marks)

b) As the ETC above is a branched pathway, the electron from NADH will be
transported to a different branch depending on the level of oxygen available in
the microbial environment. Name which branch is adopted when oxygen is
low and which when it is high. (2 marks)

c) Name substances other than oxygen that can act as the final electron acceptor
in other microorganisms. (2 marks)

B) Chemoorganotrophic microorganism can obtain their energy from organic

molecules either through fermentation, aerobic respiration or anaerobic respiration.
The yield of energy for fermentation, aerobic respiration and anaerobic respiration

a) Which process yields the highest and the lowest energy from organic
molecules? (1 mark)

b) Why anaerobic respiration yields lower energy compared to aerobic

respiration? (2 marks)