26.

14 The following data are for a liquid chromatographic column

Length of packing :24.7 cm

Flow rate :0.313 mL/min
Vm :1.37 mL
Vs :0.164 mL

A chromatpgram of a mixture of species A,B,C and D provided the following data :

Retention Time,min Width of Peak Base (W),min

Nonretained 3.1 -
A 5.4 0.41
B 13.3 1.07
C 14.1 1.16
D 21.6 1.72
Calculate :
(a) The number of plates from each peak
(b) The mean and the standard deviation for N
(c) The plate height for the column
26.15 Form the data in problem 26.14, calculate for A,B,C and D
(a) The retention factor
(b) The distribution constant
26.16 Form the data in problem 26.14, calculate for species B and C
(a) The resolution
(b) The selectivity for
(c) The length of column necessary to separate the two species with a resolution of 1.5
(d) The time required to spate the two species on the column in part (c)
26.17 Form the data in problem 26.14, calculate for species C and D
(a) The resolution
(b) The length of column necessary to separate the two species with a resolution of 1.5
 ANSWER
26.14 The following data are for a liquid chromatographic column

Length of packing :24.7 cm

Flow rate :0.313 mL/min
Vm :1.37 mL
Vs :0.164 mL

A chromatpgram of a mixture of species A,B,C and D provided the following data :

Retention Time,min Width of Peak Base (W),min

Nonretained 3.1 -
A 5.4 0.41
B 13.3 1.07
C 14.1 1.16
D 21.6 1.72

Calculate :
(a) The number of plates from each peak
N = 16 )2

NA = 16 )2 = 16 )2 = 2775.49 2775

NB = 16 )2 = 16 )2 = 2472.04 2472

NC = 16 )2 = 16 )2 = 2363.97 2363

ND = 16 )2 = 16 )2 = 2523.30 2523

(b) The mean and the standard deviation for N

The mean of N
N = = = = 2533
The Standard deviation for N
N N- N (N- N )2
2775 242 58564
2472 -61 3721
 2363 -170 28900
2523 -210 100
N = 2533 N- N )2 =
91285

= 213 200

(c) The plate height for the column

H=

H=

H = 0.0097 cm

26.15 Form the data in problem 26.14, calculate for A,B,C and D
(a) The retention factor

k=

kA = = = 0.74

kB = = = 3.3

kC = = = 3.5

kD = = =5.9 6.0

(b) The distribution constant

 K=

KA =kA = 0.74 x = 6.2

KB = kB = 3.3 x = 27,57 27

K C = kC = 3.5 x = 29,23 30

K D = kD = 6.0 x = 50.12 50

26.16 Form the data in problem 26.14, calculate for species B and C
(a) The resolution

Rs =

Rs =

Rs = 0.72
(b) The selectivity for

(c) The length of column necessary to separate the two species with a resolution of 1.5

=
 =

=
L2 = 107.20 cm
(d) The time required to spate the two species on the column in part (c)
For spesies B

= 57.7 min

For spesies C

= 61,2 min

26.17 Form the data in problem 26.14, calculate for species C and D
(a) The resolution

Rs =
 Rs =

Rs =

Rs = 5.21
(b) The length of column necessary to separate the two species with a resolution of 1.5

L2 = 2.05 cm 2 cm