Adenovirus-2

35,937 base pairs
GenBank Accession #: NC_001405
Not currently available from NEB.
Adenovirus-2, one of several serotypes of human adenoviruses,
has a linear, double-stranded DNA genome; the exact number of
genes encoded is still unknown (1). Numbering of the genome
sequence begins at the first (5-most) base of theleft end
(bottom of diagram below).
not shown. Enzymes with unique restriction sites are shown in
bold type and enzymes with two restriction sites are shown in
regular type. Location of sites of all NEB restriction enzymes
can be found on the NEB web site (choose Technical Reference
> DNA Sequences and Maps). Restriction site coordinates refer

Adenovirus-2 The map shows the positions of all characterized open reading
frames (ORFs) larger than 200 codons; locations of introns are
to the position of the 5-most base on the top strand in each
recognition sequence.

35937 bp
AvrII 35470

L5 (fiber protein)
NdeI 31076

SwaI 28904
PacI 28618
pVIII (hexon-assoc. protein)
33K (virion morphogenesis-assoc. protein)
AscI 25337 L5 (hexon assembly-assoc. protein)

BsaBI 23479 E2b (DNA-binding protein)

23 kDa (endopeptidase)
AsiSI 21482
NdeI 20624
L4 (hexon protein)
BspDI - ClaI 18657
RsrII 18648 pVI (hexon-assoc. protein)
pV (minor core protein)
AscI 15665
L3 1 (penton protein)

PmeI 13245
AvrII 13066 IIIa (peripentonal hexon-assoc. protein)

RsrII 11460 L1 (52/55 kDa protein)

BstBI 10670
E2b (terminal protein)

E2b (DNA polymerase)

PshAI 5924
E2b (virion morphogenesis-assoc. protein)
BsaBI 4051
E1b 1 (transformation-assoc. protein)
PshAI 1369
BspDI - ClaI 916 E1a (32/26 kDa protein)

References
1. Roberts, R.J. et al. (1986) in Adenovirus DNA , W. Doerfler (ed.), Martinus Nijhoff Publishing, Boston.