SAN DIEGO POLICE DEPARTMENT FORENSIC SCIENCE SECTION FORENSIC BIOLOGY UNIT LABORATORY REPORT LOPEZ, Moises DOMINGUEZ, Florencio ef a. 08051938 187%(a) PC HOMICIDE: Detective Lambert, ID #4494 DNA TECHNICAL MANAGER: Shawn Montpetit, DATE OF REPORT: August 30, 2010 SUPPLEMENTAL REPORT 4 BACKGROUND Requests were received from Detective Gasca of the Street Gang Unit for reference samples to be compared to analysis previously performed in this case. Please see my previous reports in this case dated December 23, 2008; October 19, 2009; June 1, 2010; and July 15, 2010 for additional information. EVIDENCE EXAMINED Property Tag #059250 ltem 46: Two reference mouth swabs from Roberto Ruiz, A portion of one swab was extracted for DNA analysis. Property Tag #149310 Itein JG: Two reference mouth swabs from Josue Gutierrez. A portion of one swab was extracted for DNA analysis. Note: The packaging for this item was labeled, in par “Josue Guttierez”. Property Tag #149997 Item IA: Two reference mouth swabs from Ismael! Aceves. A portion of one swab was . extracted for DNA analysis. SUMMARY OF ANALYTICAL PROCEDURES BioRobot EZ] magnetic bead-based DNA purification. Quantitative PCR of human DNA using the Quantifiler Duo kit, PCR DNA amplification using the Identifiler kit. Capillary electrophoresis and analysis of amplified DNA.FORENSIC BIOLOGY UNIT Case #08-051938 August 30, 2010 Page 2 of 8 RESULTS See Tables 1, 2, 3, and 4 (attached) for the DNA typing results. CONCLUSIONS Note: The conclusions presented in this report reflect comparisons of all newly processed reference samples to the previously analyzed evidence, For conclusions regarding all other individuals please see my previous reports. 1. The DNA recovered from the apparent bloodstain from the grass (Item 6), the swabs of Moises Lopez’ knuckles, and the fingernail scrapings (Items 29, 30, 31, and 32) is consistent with originating from Moises Lopez. Roberto Ruiz, Josue Gutierrez, and Ismael Aceves are excluded as the source of the DNA. obtained from this evidence. 2. ‘The DNA recovered from the apparent vomit (Items 14 and 14-2) matches the DNA obtained from Victor Ramos. Please refer to the report dated July 15, 2010 for the associated statistical probabilities. “Roberto Ruiz, Josue Gutierrez, and Ismael Aceves are excluded as the source of the DNA. obtained from this evidence. 3. The DNA recovered from the swab of the beer bottle (Item FS15A) and the predominant DNA recovered from the swab of the beer bottle (Item FS12A) matches the DNA obtained from Siria Ford. Please refer to the report dated July 15, 2010 for the associated statistical probabilities. “Roberto Ruiz, Josue Gutierrez, and Ismael Aceves are excluded as the source of the DNA obtained from this evidence. 4, The DNA recovered from the apparent bloodstains from the baseball hat (Item 2-1), the right and left gloves (Items 16-1 and 17-1), from 4075 Franklin Avenue (Item 40) and the predominant DNA from the swabs of the inner palms of the left glove (Item 17-2) matches the DNA obtained from Moises Lopez. Roberto Ruiz, Josue Gutierrez, and Ismael Aceves are excluded as the source of the DNA. obtained from this evidence.FORENSIC BIOLOGY UNIT Case #08-051938 August 30, 2010 Page 3 of 8 CONCLUSIONS (continued) 5. The DNA recovered from the swab of the inner palm of the right glove (Item 16-2 - 2" analysis) is a mixture of DNA from at least three people, The predominant DNA in this mixture matches the DNA obtained from Moises Lopez. Florencio Dominguez is included as a possible minor DNA contributor to the mixture. Please refer to the report dated June 1, 2010 for the associated statistical probabilities. .Due to the combination of detected DNA types in this mixture it is inconclusive as to ‘whether Roberto Ruiz or Josue Gutierrez could be possible minor DNA contributors to this mixture. Ismael Aceves is excluded as a possible DNA contributor to the DNA obtained from this evidence. 6. The DNA recovered from the apparent bloodstains from the left shoulder of the shirt (Item 37-1), from Victor Ramos” shirt (Item 43B-1), the shirt found at 4075 Franklin Avenue (tems 44-1 and 44-2), and the DNA recovered from the swab of the beer bottle (Item FS9A) is from an unknown male, Roberto Ruiz, Josue Gutierrez, and Ismael Aceves are excluded as the source of the DNA obtained from this evidence. 7, ‘The DNA recovered from the swab of the beer bottle (Item FS13A) is from a second ‘unknown male. Roberto Ruiz, Josue Gutierrez, and Ismael Aceves are excluded as the source of the DNA obtained from this evidence, 8. A low level of DNA was recovered ftom the swab of the collar of the shirt recovered at 4075 Franklin Avenue (Item 44-3). The results are too low to make any conclusions regarding this sample. 9, The DNA recovered from the swab of the beer bottle (Item FS8A) and the predominant DNA from the swab of the beer bottle (Item FS10A) matches the DNA obtained from Florencio Dominguez. Please refer to the report dated June 1, 2010 for the associated statistical probabilities, Roberto Ruiz, Josue Gutierrez, and Ismael Aceves are excluded as the source of the DNA “obtained from this evidence.FORENSIC BIOLOGY UNIT Case #08-051938 August 30, 2010 Page 4 of 8 . CONCLUSIONS (continued) 10. A mixture of DNA from at least three people was recovered from the swab of the collar area of the shirt (Item 37-2). Victor Ramos is included as a possible major DNA contributor to the mixture. Evelyn Soto and Siria Ford could not be excluded as possible minor DNA contributors to this mixture. Due to the combination of DNA types detected in the mixture itis inconclusive as to whether Roberto Ruiz could be a possible minor DNA contributor to this mixture. Ismael Aceves and Josie Gutierrez are excluded as contributors to the mixture of DNA obtained from this evidence. DISPOSITION OF EVIDENCE, ‘The reference samples were returned to the Property Room. The extracted DNA was submitted to the Property Room under Property Tag #148534. If you have any questions regarding this report, please fee! free to contact me. wi Montpetit, ID #8673 DNA Technical Manager August 30, 2010 Technical Review: Date: 413/10 4afo. Administrative Review:FORENSIC BIOLOGY UNIT Case # 08-051938 August 30,2010 Page 5 of 8. a Te le 1: DNA typing results. oo a _ em [DIST] DRSII, THAT SING] DIgiasaT “TAUL pmass | ONE iB] DISSHRR. | Ay SER DABS freee) 11S | 29) 11) 2 1,12 is | ie) tt | 17 | XY tontetes ‘ucretmse) 15) 29,382 | To, | zu 1a | we | a1 | mis PRY) on] Bs fontinat [16 Reference! 322 | 112 [10,12 | 1618 | 8,9 | 9,12 1 16,23 | 13,132 | 1619 | 8,11 | 13,18 | xy | 11,12 9 tome | Care | _ ‘Table 2; DNA typing results from report dated July 15,2010, oe 5S RE Se, Fa OT] ESN sen, OSES | ALLA ST [| 1 90,322 9310/12 Is 67) 3,10 | 10,2) 7 | i4ie2 | igi? | 8 | as 7 x¥ | 1] 28,28 3312 2 a9 Te | eM | we 7 2,28 Baia |The |e %9 | 10,12 | Woe | 2,38 [apy Bm 30,322 | 93,10 | 11,12 15 67 | 9,10 | 10,12 7 14,162 | 16,17 8 3) xy i 23,24 30,22 [9310/1] Is | 67 | a} em |W | wie] | 8 | [x] | a2 30,22 [93,10 | 1,2) 15 | 67 |W | 1a) 7 | wiea ie) 8 | Ter] oe 30,322 | 93° | 1,12]; as 67 | 9,10 | 10,12 v7 14,162 | 16,17 8 B |xY| i 2a* 322,332 | 8,9 W 13,15 [7,93 | 11,12 12 23,25 B 16,20 | 8,9 | 14,15 | x 21,22 * = Due tothe intensity ofthe result allelic drop-out cannot be ruled out at this markerFORENSIC BIOLOGY UNIT Case # 08-051938 August 30, 2010 Page 6 of 8 _ 7 Table 2: DNA typing results (continued). _ a _ Item Das. [DISH eSrPO] Baise] TAO [as 1aSe59 BOS Iaea] DAIGG: | MWA.) Tron | Digssi| amnel/ DSH [RGA | I Reece 29,30 | 9,10 | 1,11] 1516 | 6 | 912 | 9,10 | 24,95 | 14,142 16,17 | 8 Tint? | XY 11 | 24,26 lee ert (49: Reference | 13,14 | 27,322 1 12,13 | 14,15 6,8 1,13 10,12 | 23,25 12,15) 35,17 [8 | 14,15 | X¥ | 1,13 | 25,26 | ‘em ee Pane [Skea] 11S [292 | RN | eB | Ie) 6&7 | wR] wR) ae) wis | wm | a9 fie) x) an | a ‘om ite | ‘iReteence | 13,14 | 30,322 10,11 | 10,11 | 15,16 7,9 811 W413 | 24,26 | 132,16 | 16,17 | 11,12 | 12,16 | x¥ | 11,12 | 19,23 tonto ‘7: Reference | 10,14 | 30,312 | 10,11 | 10,12) 1S 7,93 9,13, 813 | 17,24 | 132,152 | 16,18 | &12 | 11,16 | 12,13 | 18,22, ‘om Andes bee _ | Rete! 415 | 99,30 | ON [RB ww | 693 | |i | me) | wi | a pina |x] Ton] ma fm Gree | Nene | 30 9,12 | 13,16 | 7 9 wa | on Bie) ne | ‘Table 3: DNA typing results from ssi | iio) Dasiasa! am [ipvissig| pu 30,322 1} | 1s 67 9,10 32 ie | oF BaF a | [ae 2,32 mie 68 | aaa | ssa | I | BIO | 1425 | XY | WOT | 2526FORENSIC BIOLOGY UNIT Case # 08-051938 August 30, 2010 Page 7 of 8 _Table 4: DNA typing results from previously analyzed evidence items (see report dated December 23, 2008) liga». [DASii79 | DPISHL | DS#0 SELES] DISIANE |. TAO amIaNsie | mage | DSSS Is DSSRIR: | oRGA. rape | | steae [oad Pie | sper tate Pi Plane eo nm] 220 Tetx senor WISE a | ae | a | ss Tae | iaas Paar wi helen ox sen Tiss 7) a ana | waa ee BB ivaaed waa Tie | ee a sa ai | as XY 36 pron sth se or wee | a7 [wae | tas | as | as] as] ae | ta x B ones 8 vcd cs saat ie | aa 3 piss | ae | | ae | |e [SISA Swab of | 2931.2 16,18 67 10,12 2 RIS 15 89 1617 |X 7 3 | ‘eben I | 164: Agere) 3032 5 67 | 940 7 | ated | et |e B > RY) on | aa coane | 16.2: Swab of | 30,322 93,10 | 11,12) is 67 1617 8 B XY 23,24 econ 09292 | wkl2 wats | wtta9) wai ‘cause iz mnet 30,382 | 93,10 | a] Yer a | a] Bae en 28, vias,| wis, ws, | wk io was | wato | ois was waa, | an, war, word | wiz wie 17: Apparen | 13 30,322 93,10 | U2 is 6.7 16,17, 8 B XY n Bm) eng a soxa | 9380 | tae | isa? | a0] a) Fr ocr ware | wis | wzt3) wito wito inate 8: Ape usa [om | a [is | 9 | aie | ea) ia | isie | tee | as) a par mas aie |FORENSIC BIOLOGY UNIT Case # 08-051938 August 30, 2010 Page 8 of 8 aa 870: a _Table 4 (continued): DNA typing results (see report dated December 23, 2008). 7 well 32 Swibot leectar aan me || er (sei ames] IAS | 3332) or) |S 7 1720 wy 2s wr arene | EAS [ 34332] Sa) a] as 7 1720 156 1618 TAT cag ToT aia ua apes | EIS | 30382 | our | a) Is 7 ai aD 1720 ary 1618 TAT ToT es Bie | ND | ND | is | a7 Ww ™D WD §D §D ND Wk= Weak DNA types (<1 the intensity ofthe tallest DNA type at that marker) ND=No DNA types detected "= Results ftom report dated December 23, 2008 >= Results from report dated June 1, 2010 ‘The tables of results represents a summary ofthe detected DNA types in the samples). Interpretations and conclusions reported are based upon a review of the actual data,